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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U03473-1
Contig ID Contig-U03473-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U03473-1 (Contig-U03473-1Q) /CSM_Contig/Contig-U03473-1Q.Seq.d
AGTTTTGGAAAAANATGGNAAAACTAATNGNATGATTGAANCNATTAAAG
NATTTGGNGAAANAATTAANCAACGNTGGNTCAAAAANATTCCAATCNTT
NTTTTTTTAAANAAACGTGANTTATTCGCAAANAAAANTAAAAAAACTGG
NATTTCAANTTNNTTCC
Gap no gap
Contig length 167
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 1
Number of EST 1
Link to clone list U03473
List of clone(s) 

est1=SFD447Z,1,168
Translated Amino Acid sequence 
VLEKXGKTNXMIEXIKXFGEXIXQRWXKXIPIXXFLXKRXLFAXKXKKTGISXXF
Translated Amino Acid sequence (All Frames) 
Frame A:
sfgkxwxn*xxd*xx*xiwxxn*xtxxqkxsnxxffkxt*xirkxx*knwxfxxx


Frame B:
VLEKXGKTNXMIEXIKXFGEXIXQRWXKXIPIXXFLXKRXLFAXKXKKTGISXXF


Frame C:
fwkxmxklxx*lxxlkxlxkxlxnxgskxfqsxff*xnvxysqxkxkklxfqxxs
own update 2004. 6. 9
Homology vs CSM-cDNA 
Query= Contig-U03473-1 (Contig-U03473-1Q)
/CSM_Contig/Contig-U03473-1Q.Seq.d
         (167 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U03473-1 (Contig-U03473-1Q) /CSM_Contig/Conti...   115  1e-26

>Contig-U03473-1 (Contig-U03473-1Q) /CSM_Contig/Contig-U03473-1Q.Seq.d
          Length = 167

 Score =  115 bits (58), Expect = 1e-26
 Identities = 97/97 (100%)
 Strand = Plus / Plus

                                                                      
Query: 1  agttttggaaaaanatggnaaaactaatngnatgattgaancnattaaagnatttggnga 60
          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  agttttggaaaaanatggnaaaactaatngnatgattgaancnattaaagnatttggnga 60

                                               
Query: 61 aanaattaancaacgntggntcaaaaanattccaatc 97
          |||||||||||||||||||||||||||||||||||||
Sbjct: 61 aanaattaancaacgntggntcaaaaanattccaatc 97


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 99
Number of Sequences: 6905
Number of extensions: 99
Number of successful extensions: 14
Number of sequences better than 10.0: 1
length of query: 167
length of database: 5,674,871
effective HSP length: 15
effective length of query: 152
effective length of database: 5,571,296
effective search space: 846836992
effective search space used: 846836992
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2009. 6. 1
Homology vs DNA 
Query= Contig-U03473-1 (Contig-U03473-1Q) /CSM_Contig/Contig-U03473-1Q.Seq.d
         (167 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ399773) Dictyostelium discoideum cDNA clone:dds7n12, 3' e...   115   4e-28  2
(AC114261) Dictyostelium discoideum chromosome 2 map 126059-...    92   7e-15  1

>(BJ399773) Dictyostelium discoideum cDNA clone:dds7n12, 3' end,
           single read.
          Length = 168

 Score =  115 bits (58), Expect(2) = 4e-28
 Identities = 97/97 (100%)
 Strand = Plus / Minus


Query: 1   agttttggaaaaanatggnaaaactaatngnatgattgaancnattaaagnatttggnga 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 168 agttttggaaaaanatggnaaaactaatngnatgattgaancnattaaagnatttggnga 109


Query: 61  aanaattaancaacgntggntcaaaaanattccaatc 97
           |||||||||||||||||||||||||||||||||||||
Sbjct: 108 aanaattaancaacgntggntcaaaaanattccaatc 72

 Score = 40.1 bits (20), Expect(2) = 4e-28
 Identities = 29/29 (100%)
 Strand = Plus / Minus


Query: 109 aaanaaacgtganttattcgcaaanaaaa 137
           |||||||||||||||||||||||||||||
Sbjct: 60  aaanaaacgtganttattcgcaaanaaaa 32

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 23,697,989
Number of extensions: 1434471
Number of successful extensions: 131693
Number of sequences better than 10.0: 2
Length of query: 167
Length of database: 101,790,757,118
Length adjustment: 22
Effective length of query: 145
Effective length of database: 99,544,435,898
Effective search space: 14433943205210
Effective search space used: 14433943205210
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 7.28
Homology vs Protein 
Query= Contig-U03473-1 (Contig-U03473-1Q) /CSM_Contig/Contig-U03473-1Q.Seq.d
         (167 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34045) RecName: Full=Guanine nucleotide-binding protein alpha-...    72   5e-12
L33847_1(L33847|pid:none) Dictyostelium discoideum G protein alp...    67   1e-10
T23705(T23705)hypothetical protein M04C7.1 - Caenorhabditis eleg...    40   0.026
(P28052) RecName: Full=Guanine nucleotide-binding protein alpha-...    39   0.075
(Q60W52) RecName: Full=Guanine nucleotide-binding protein alpha-...    39   0.075
(Q60XS3) RecName: Full=Guanine nucleotide-binding protein alpha-...    37   0.17
(Q4VT35) RecName: Full=Guanine nucleotide-binding protein alpha-...    37   0.17
AF292562_1(AF292562|pid:none) Strongyloides stercoralis G protei...    37   0.22
(P22454) RecName: Full=Guanine nucleotide-binding protein alpha-...    37   0.28
(P20353) RecName: Full=Guanine nucleotide-binding protein G(i) s...    35   0.63
S47614_1(S47614|pid:none) G-protein alpha i subunit [Homarus ame...    34   1.8
DQ182016_1(DQ182016|pid:none) Anopheles gambiae G(alpha)i mRNA, ...    32   5.3
AB022098_1(AB022098|pid:none) Halocynthia roretzi mRNA for G pro...    32   5.3
AY542969_1(AY542969|pid:none) Bigelowiella natans guanine nucleo...    32   7.0
FJ230781_1(FJ230781|pid:none) Drechslerella dactyloides G protei...    32   7.0
(Q9N2V6) RecName: Full=Guanine nucleotide-binding protein alpha-...    32   9.1

>(P34045) RecName: Full=Guanine nucleotide-binding protein alpha-7
           subunit;          Short=G alpha-7;
           &AC114261_8(AC114261|pid:none)
          Length = 390

 Score = 72.4 bits (176), Expect = 5e-12
 Identities = 37/55 (67%), Positives = 39/55 (70%)
 Frame = +2

Query: 2   VLEKXGKTNXMIEXIKXFGEXIXQRWXKXIPIXXFLXKRXLFAXKXKKTGISXXF 166
           VLE+ GKTN MIE IK FG+ I QRW K IPI  FL KR LFA K KKTGIS  F
Sbjct: 271 VLEEDGKTNRMIESIKVFGDIINQRWFKDIPIILFLNKRDLFAEKIKKTGISICF 325

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 78,054,435
Number of extensions: 379306
Number of successful extensions: 118
Number of sequences better than 10.0: 16
Number of HSP's gapped: 118
Number of HSP's successfully gapped: 16
Length of query: 55
Length of database: 1,061,185,681
Length adjustment: 28
Effective length of query: 27
Effective length of database: 969,669,137
Effective search space: 26181066699
Effective search space used: 26181066699
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 26 (14.6 bits)
PSORT 

 psg: 0.73  gvh: 0.45  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U03473-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 1
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0