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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U16543-1
Contig ID Contig-U16543-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U16543-1 (Contig-U16543-1Q) /CSM_Contig/Contig-U16543-1Q.Seq.d
TTATCTTTCAATGAATTCAAACAATTCGCAATGTCTCATCCAAAAATTAC
TGCCACATTAAATGGTTCAAAGAGAGACGTGCCAATTACTTTTGATTAAA
TTAATCATACAACCATTTCTTTATTAATAAAAAAAATCAAAAAAAAAAAG
TATATAAATAAATTGTAATTAATAGATTTTAACTAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA----------ACAAAA
CCAACACACACTCAGCATTTACAAAAAAAAAAAAAAAACTTTTGGTTTAT
TGTTTTAAAATCAATCACATAATTAAAAATGGAAGACACACAAGAACAAA
TTGAACAAGTTGAACAACAAGTTGAAGAAACTGTTGAAAAAGTTAAAGAA
GAAGCAACAGAGAAAGTTGAAGCAGCAATTGAAGAAGTTAAAGAAGATAT
CAATAAATTAAAGAATGATGTAAAGGGAGTCGCAGAGAACGTTAGTAATG
TAGTCTCATCAAAGATTGAAGCAACCACATCATCAGCAAAGGCCACCATT
AATGATATTAAGGATGGCGTCAAACAAGTTGTATCTGGTAATTCTGGTTG
TCCATACTCTATGAATGGTGAATTCTCAATGTGTAGCCAAGTTTGCAAAG
TCAGCAAAGTAGTTCAAAAGAATCCATC
Gap gap included
Contig length 668
Chromosome number (1..6, M) 6
Chromosome length 3595308
Start point 2438473
End point 2437887
Strand (PLUS/MINUS) MINUS
Number of clones 79
Number of EST 101
Link to clone list U16543
List of clone(s) 

est1=SSG694E,1,234
est2=VSA738E,9,234
est3=AHQ590Z,12,234
est4=AHQ642Z,12,234
est5=SSG160E,13,234
est6=AHG261F,19,234
est7=AHG261Z,19,234
est8=VSH381F,24,234
est9=AHQ590F,26,234
est10=AHQ642F,26,234
est11=SSM363E,26,234
est12=SHB431F,41,234
est13=VSB292E,44,234
est14=VSC718E,44,234
est15=VFC129F,46,234
est16=SSD373Z,47,234
est17=AHJ449E,51,234
est18=VSI646Z,55,234
est19=AHQ740E,57,234
est20=SHB431Z,57,234
est21=VSD645Z,57,234
est22=VSD665Z,57,234
est23=VSD821Z,57,234
est24=VSJ416Z,57,234
est25=SSM341E,58,234
est26=VSH381Z,58,234
est27=VSK430Z,59,234
est28=AHE328F,60,234
est29=SSE281E,61,234
est30=SSM584E,61,234
est31=AHQ460F,62,234
est32=SSA493F,62,234
est33=SSB789E,62,234
est34=SSC339E,62,234
est35=SSG716E,62,234
est36=SSE809F,63,234
est37=SSL393F,63,234
est38=VFE203F,63,234
est39=VFE203Z,63,234
est40=VSJ416F,64,234
est41=AFO644F,66,234
est42=AHG416F,66,234
est43=AHG416Z,66,234
est44=VSI646F,66,234
est45=SSI310E,67,234
est46=VHN654Z,67,234
est47=SHD801F,68,234
est48=SHD801Z,68,234
est49=VHN654F,68,234
est50=VSD645F,68,234
est51=VSD665F,68,234
est52=VSD821F,68,234
est53=SSC889E,69,234
est54=AFD630F,70,234
est55=SSD338E,70,234
est56=SSJ564E,70,234
est57=VSK430F,70,234
est58=SSC681F,71,234
est59=SSK844E,71,234
est60=SSG452F,74,234
est61=SSG709E,74,234
est62=SSI316E,74,234
est63=SSJ847E,75,234
est64=SSC431Z,76,234
est65=SSH388E,76,234
est66=SSL624E,83,234
est67=VSJ742F,83,234
est68=SSC827E,84,234
est69=SSB656E,85,234
est70=VSE579Z,86,234
est71=VSF658Z,87,234
est72=VSG372Z,88,234
est73=VSH762F,89,234
est74=VSJ742Z,89,234
est75=VSF259F,90,234
est76=VSJ595Z,91,234
est77=SSB457Z,94,234
est78=SSF252E,97,234
est79=SSC745E,98,234
est80=SSG815F,98,234
est81=VSF658F,98,234
est82=VSJ595F,98,234
est83=SSE550E,99,234
est84=SSG740E,99,234
est85=SSI443E,99,234
est86=VSB306E,99,234
est87=SLG107E,100,234
est88=SSL235E,100,234
est89=VSF839F,100,234
est90=VSG372F,100,234
est91=VSA356E,104,234
est92=SSK602E,107,234
est93=VSF839Z,112,234
est94=VSD406F,117,234
est95=VSF566F,117,234
est96=SSG617E,122,234
est97=SSE474Z,131,234
est98=SSA743E,142,234
est99=VSC167Z,157,234
est100=AHE328Z,204,234
est101=AFD630Z,245,356
Translated Amino Acid sequence 
ylsmnsnnsqcliqkllph*mvqretcqlllikliiqpfly**kkskkksi*incn**IL
TKKKKKKKKKKKKKKKK---

---KTNTHSAFTKKKKKLLVYCFKINHIIKNGRHTRTN*ts*tts*rnc*ks*rrsnres
*ssn*rs*rryq*ike*ckgsrrer**cslikd*snhiiskghh**y*gwrqtsciw*fw
lsilyew*ilnv*pslqsqqssskesi
Translated Amino Acid sequence (All Frames) 
Frame A:
lsfnefkqfamshpkitatlngskrdvpitfd*inhttisllikkikkkkyinkl*lidf
n*kkkkkkkkkkkkkkkk---

---tkpthtqhlqkkkknfwfivlksit*lkmedtqeqieqveqqveetvekvkeeatek
veaaieevkedinklkndvkgvaenvsnvvsskieattssakatindikdgvkqvvsgns
gcpysmngefsmcsqvckvskvvqknp

Frame B:
ylsmnsnnsqcliqkllph*mvqretcqlllikliiqpfly**kkskkksi*incn**IL
TKKKKKKKKKKKKKKKK---

---qnqhtlsiykkkkktfgllf*nqshn*kwkthknklnklnnklkkllkklkkkqqrk
lkqqlkklkkisin*rmm*resqrtlvm*shqrlkqphhqqrpplmilrmasnklylvil
vvhtl*mvnsqcvakfaksak*fkrih

Frame C:
ifq*iqtirnvssknychikwfkerranyf*ln*synhffinkknqkkkvyk*ivinrf*
lkkkkkkkkkkkkkkkk---

---KTNTHSAFTKKKKKLLVYCFKINHIIKNGRHTRTN*ts*tts*rnc*ks*rrsnres
*ssn*rs*rryq*ike*ckgsrrer**cslikd*snhiiskghh**y*gwrqtsciw*fw
lsilyew*ilnv*pslqsqqssskesi
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U16543-1 (Contig-U16543-1Q)
/CSM_Contig/Contig-U16543-1Q.Seq.d
         (678 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U16544-1 (Contig-U16544-1Q) /CSM_Contig/Conti...   458  e-129
Contig-U16543-1 (Contig-U16543-1Q) /CSM_Contig/Conti...   458  e-129
Contig-U14343-1 (Contig-U14343-1Q) /CSM_Contig/Conti...   432  e-121
Contig-U16542-1 (Contig-U16542-1Q) /CSM_Contig/Conti...   254  2e-67
Contig-U14128-1 (Contig-U14128-1Q) /CSM_Contig/Conti...    56  7e-08
Contig-U13295-1 (Contig-U13295-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U09164-1 (Contig-U09164-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U08146-1 (Contig-U08146-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U11446-1 (Contig-U11446-1Q) /CSM_Contig/Conti...    38  0.017
Contig-U15543-1 (Contig-U15543-1Q) /CSM_Contig/Conti...    36  0.068

>Contig-U16544-1 (Contig-U16544-1Q) /CSM_Contig/Contig-U16544-1Q.Seq.d
          Length = 1126

 Score =  458 bits (231), Expect = e-129
 Identities = 231/231 (100%)
 Strand = Plus / Plus

                                                                       
Query: 448 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 507
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 204 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 263

                                                                       
Query: 508 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 567
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 264 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 323

                                                                       
Query: 568 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 627
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 324 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 383

                                                              
Query: 628 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 678
           |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 384 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 434


 Score =  188 bits (95), Expect = 8e-48
 Identities = 95/95 (100%)
 Strand = Plus / Plus

                                                                       
Query: 289 cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacacacaagaaca 348
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 45  cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacacacaagaaca 104

                                              
Query: 349 aattgaacaagttgaacaacaagttgaagaaactg 383
           |||||||||||||||||||||||||||||||||||
Sbjct: 105 aattgaacaagttgaacaacaagttgaagaaactg 139


 Score = 56.0 bits (28), Expect = 7e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 245 acaaaaccaacacacactcagcatttac 272
           ||||||||||||||||||||||||||||
Sbjct: 1   acaaaaccaacacacactcagcatttac 28


>Contig-U16543-1 (Contig-U16543-1Q) /CSM_Contig/Contig-U16543-1Q.Seq.d
          Length = 678

 Score =  458 bits (231), Expect = e-129
 Identities = 231/231 (100%)
 Strand = Plus / Plus

                                                                       
Query: 448 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 507
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 448 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 507

                                                                       
Query: 508 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 567
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 508 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 567

                                                                       
Query: 568 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 627
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 568 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 627

                                                              
Query: 628 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 678
           |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 628 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 678


 Score =  254 bits (128), Expect = 2e-67
 Identities = 128/128 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   ttatctttcaatgaattcaaacaattcgcaatgtctcatccaaaaattactgccacatta 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   ttatctttcaatgaattcaaacaattcgcaatgtctcatccaaaaattactgccacatta 60

                                                                       
Query: 61  aatggttcaaagagagacgtgccaattacttttgattaaattaatcatacaaccatttct 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aatggttcaaagagagacgtgccaattacttttgattaaattaatcatacaaccatttct 120

                   
Query: 121 ttattaat 128
           ||||||||
Sbjct: 121 ttattaat 128


 Score =  188 bits (95), Expect = 8e-48
 Identities = 95/95 (100%)
 Strand = Plus / Plus

                                                                       
Query: 289 cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacacacaagaaca 348
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 289 cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacacacaagaaca 348

                                              
Query: 349 aattgaacaagttgaacaacaagttgaagaaactg 383
           |||||||||||||||||||||||||||||||||||
Sbjct: 349 aattgaacaagttgaacaacaagttgaagaaactg 383


 Score = 69.9 bits (35), Expect = 5e-12
 Identities = 35/35 (100%)
 Strand = Plus / Plus

                                              
Query: 150 gtatataaataaattgtaattaatagattttaact 184
           |||||||||||||||||||||||||||||||||||
Sbjct: 150 gtatataaataaattgtaattaatagattttaact 184


 Score = 56.0 bits (28), Expect = 7e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 245 acaaaaccaacacacactcagcatttac 272
           ||||||||||||||||||||||||||||
Sbjct: 245 acaaaaccaacacacactcagcatttac 272


>Contig-U14343-1 (Contig-U14343-1Q) /CSM_Contig/Contig-U14343-1Q.Seq.d
          Length = 1375

 Score =  432 bits (218), Expect = e-121
 Identities = 218/218 (100%)
 Strand = Plus / Plus

                                                                       
Query: 461 aagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctcatcaaagattgaa 520
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctcatcaaagattgaa 60

                                                                       
Query: 521 gcaaccacatcatcagcaaaggccaccattaatgatattaaggatggcgtcaaacaagtt 580
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  gcaaccacatcatcagcaaaggccaccattaatgatattaaggatggcgtcaaacaagtt 120

                                                                       
Query: 581 gtatctggtaattctggttgtccatactctatgaatggtgaattctcaatgtgtagccaa 640
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gtatctggtaattctggttgtccatactctatgaatggtgaattctcaatgtgtagccaa 180

                                                 
Query: 641 gtttgcaaagtcagcaaagtagttcaaaagaatccatc 678
           ||||||||||||||||||||||||||||||||||||||
Sbjct: 181 gtttgcaaagtcagcaaagtagttcaaaagaatccatc 218


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 11,810
Number of Sequences: 8402
Number of extensions: 11810
Number of successful extensions: 1148
Number of sequences better than 10.0: 136
length of query: 678
length of database: 8,075,542
effective HSP length: 16
effective length of query: 662
effective length of database: 7,941,110
effective search space: 5257014820
effective search space used: 5257014820
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 4.12
Homology vs DNA 
Query= Contig-U16543-1 (Contig-U16543-1Q) /CSM_Contig/Contig-U16543-1Q.Seq.d
         (678 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU060949) Dictyostelium discoideum slug cDNA, clone SLC352.      458   e-150  3
(BJ409820) Dictyostelium discoideum cDNA clone:ddv10k04, 5' ...   458   e-148  2
(BJ413289) Dictyostelium discoideum cDNA clone:ddv14p19, 5' ...   458   e-147  2
(BJ411446) Dictyostelium discoideum cDNA clone:ddv4p09, 5' e...   458   e-146  2
(BJ327375) Dictyostelium discoideum cDNA clone:dda20o06, 5' ...   458   e-141  2
(BJ331996) Dictyostelium discoideum cDNA clone:dda37h24, 5' ...   458   e-141  2
(BJ327344) Dictyostelium discoideum cDNA clone:dda20i04, 5' ...   458   e-141  2
(BJ328455) Dictyostelium discoideum cDNA clone:dda28b10, 5' ...   458   e-141  2
(BJ391275) Dictyostelium discoideum cDNA clone:dds15m24, 5' ...   458   e-141  2
(BJ413136) Dictyostelium discoideum cDNA clone:ddv14f12, 5' ...   458   e-140  3
(BJ412075) Dictyostelium discoideum cDNA clone:ddv6j22, 5' e...   458   e-140  2
(BJ336754) Dictyostelium discoideum cDNA clone:dda55d05, 5' ...   450   e-139  2
(BJ415195) Dictyostelium discoideum cDNA clone:ddv21c22, 5' ...   458   e-135  2
(BJ416493) Dictyostelium discoideum cDNA clone:ddv26i14, 5' ...   458   e-133  2
(BJ326769) Dictyostelium discoideum cDNA clone:dda17n22, 5' ...   458   e-133  2
(BJ325305) Dictyostelium discoideum cDNA clone:dda13a23, 5' ...   458   e-133  2
(BJ415063) Dictyostelium discoideum cDNA clone:ddv21f11, 5' ...   458   e-131  2
(BJ414626) Dictyostelium discoideum cDNA clone:ddv19k21, 5' ...   458   e-130  2
(BJ411264) Dictyostelium discoideum cDNA clone:ddv3m15, 5' e...   458   e-128  2
(AU261395) Dictyostelium discoideum vegetative cDNA clone:VS...   432   e-117  1
(BJ354874) Dictyostelium discoideum cDNA clone:dda55d05, 3' ...   371   2e-98  1
(BJ432003) Dictyostelium discoideum cDNA clone:ddv17e12, 3' ...   321   2e-83  1
(BJ435699) Dictyostelium discoideum cDNA clone:ddv27o22, 3' ...   301   2e-77  1
(BJ401039) Dictyostelium discoideum cDNA clone:dds15m24, 3' ...   301   2e-77  1
(BJ374048) Dictyostelium discoideum cDNA clone:ddc6d07, 3' e...   301   2e-77  1
(BJ349852) Dictyostelium discoideum cDNA clone:dda37h24, 3' ...   301   2e-77  1
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...   254   4e-63  1
(AU263271) Dictyostelium discoideum vegetative cDNA clone:VS...   254   4e-63  1
(AU261685) Dictyostelium discoideum vegetative cDNA clone:VS...   254   4e-63  1
(AU076378) Dictyostelium discoideum slug cDNA, clone SSA674.      254   4e-63  1
(AU037957) Dictyostelium discoideum slug cDNA, clone SSE493.      254   4e-63  1
(C94366) Dictyostelium discoideum slug cDNA, clone SSK890.        254   4e-63  1
(C94352) Dictyostelium discoideum slug cDNA, clone SSK870.        254   4e-63  1
(C94285) Dictyostelium discoideum slug cDNA, clone SSK738.        254   4e-63  1
(C94223) Dictyostelium discoideum slug cDNA, clone SSK561.        254   4e-63  1
(C93888) Dictyostelium discoideum slug cDNA, clone SSL831.        254   4e-63  1
(C92500) Dictyostelium discoideum slug cDNA, clone SSE854.        254   4e-63  1
(C91985) Dictyostelium discoideum slug cDNA, clone SSC670.        254   4e-63  1
(C91558) Dictyostelium discoideum slug cDNA, clone SSK441.        254   4e-63  1
(C90926) Dictyostelium discoideum slug cDNA, clone SSJ317.        254   4e-63  1
(C83920) Dictyostelium discoideum slug cDNA, clone SSA634.        248   2e-61  1
(BJ431200) Dictyostelium discoideum cDNA clone:ddv13p04, 3' ...   244   4e-60  1
(BJ324842) Dictyostelium discoideum cDNA clone:dda8g12, 5' e...   178   4e-60  2
(AU037289) Dictyostelium discoideum slug cDNA, clone SSB722.      242   1e-59  1
(BJ346260) Dictyostelium discoideum cDNA clone:dda30k07, 3' ...   234   4e-57  1
(BJ375393) Dictyostelium discoideum cDNA clone:ddc18e18, 3' ...   230   6e-56  1
(BJ347146) Dictyostelium discoideum cDNA clone:dda26b10, 3' ...   230   6e-56  1
(BJ344775) Dictyostelium discoideum cDNA clone:dda20o12, 3' ...   228   2e-55  1
(BJ430307) Dictyostelium discoideum cDNA clone:ddv6d23, 3' e...   224   3e-54  1
(AU263610) Dictyostelium discoideum vegetative cDNA clone:VS...   182   4e-54  2
(BJ375437) Dictyostelium discoideum cDNA clone:ddc18p14, 3' ...   168   4e-54  2
(BJ432697) Dictyostelium discoideum cDNA clone:ddv19p10, 3' ...   222   1e-53  1
(BJ431342) Dictyostelium discoideum cDNA clone:ddv13f24, 3' ...   222   1e-53  1
(BJ344698) Dictyostelium discoideum cDNA clone:dda20o06, 3' ...   222   1e-53  1
(BJ429664) Dictyostelium discoideum cDNA clone:ddv4p09, 3' e...   220   5e-53  1
(C90822) Dictyostelium discoideum slug cDNA, clone SSJ188.        216   8e-52  1
(BJ429486) Dictyostelium discoideum cDNA clone:ddv3m15, 3' e...   210   5e-50  1
(BJ400758) Dictyostelium discoideum cDNA clone:dds14m18, 3' ...   208   2e-49  1
(BJ344670) Dictyostelium discoideum cDNA clone:dda20i04, 3' ...   208   2e-49  1
(BJ428524) Dictyostelium discoideum cDNA clone:ddv12e09, 3' ...   190   5e-44  1
(AU072218) Dictyostelium discoideum slug cDNA, clone SSD718.      103   7e-44  3
(AU071339) Dictyostelium discoideum slug cDNA, clone SSB255.      103   7e-44  3
(BJ346668) Dictyostelium discoideum cDNA clone:dda24k15, 3' ...   188   2e-43  1
(BJ430330) Dictyostelium discoideum cDNA clone:ddv6j22, 3' e...   172   1e-38  1
(BJ398315) Dictyostelium discoideum cDNA clone:dds11o23, 3' ...   172   1e-38  1
(BJ444387) Dictyostelium discoideum cDNA clone:ddv56p06, 3' ...   168   2e-37  1
(BJ431476) Dictyostelium discoideum cDNA clone:ddv14f12, 3' ...   168   2e-37  1
(BJ397965) Dictyostelium discoideum cDNA clone:dds10n08, 3' ...   168   2e-37  1
(BJ341824) Dictyostelium discoideum cDNA clone:dda8g12, 3' e...   167   7e-37  1
(BJ372992) Dictyostelium discoideum cDNA clone:ddc15b11, 3' ...   165   3e-36  1
(BJ352761) Dictyostelium discoideum cDNA clone:dda47h19, 3' ...   165   3e-36  1
(BJ434720) Dictyostelium discoideum cDNA clone:ddv24c21, 3' ...   163   1e-35  1
(BJ372236) Dictyostelium discoideum cDNA clone:ddc11e23, 3' ...   163   1e-35  1
(BJ345615) Dictyostelium discoideum cDNA clone:dda28b10, 3' ...   163   1e-35  1
(BJ433277) Dictyostelium discoideum cDNA clone:ddv21f11, 3' ...   161   4e-35  1
(BJ342383) Dictyostelium discoideum cDNA clone:dda13a23, 3' ...   161   4e-35  1
(BJ433409) Dictyostelium discoideum cDNA clone:ddv21c22, 3' ...   159   2e-34  1
(BJ432803) Dictyostelium discoideum cDNA clone:ddv19k21, 3' ...   159   2e-34  1
(BJ432047) Dictyostelium discoideum cDNA clone:ddv17o08, 3' ...   159   2e-34  1
(BJ342153) Dictyostelium discoideum cDNA clone:dda9p15, 3' e...   159   2e-34  1
(BJ444645) Dictyostelium discoideum cDNA clone:ddv57d01, 3' ...   157   7e-34  1
(BJ434541) Dictyostelium discoideum cDNA clone:ddv24n01, 3' ...   157   7e-34  1
(BJ378040) Dictyostelium discoideum cDNA clone:ddc26j23, 3' ...   157   7e-34  1
(BJ434064) Dictyostelium discoideum cDNA clone:ddv23e20, 3' ...   155   3e-33  1
(BJ429383) Dictyostelium discoideum cDNA clone:ddv3e11, 3' e...   153   1e-32  1
(BJ378006) Dictyostelium discoideum cDNA clone:ddc26b22, 3' ...   151   4e-32  1
(BJ401877) Dictyostelium discoideum cDNA clone:dds19o13, 3' ...   149   2e-31  1
(BJ399244) Dictyostelium discoideum cDNA clone:dds5d03, 3' e...   149   2e-31  1
(BJ433196) Dictyostelium discoideum cDNA clone:ddv21f05, 3' ...   147   6e-31  1
(BJ435268) Dictyostelium discoideum cDNA clone:ddv26i14, 3' ...   117   2e-30  2
(BJ435554) Dictyostelium discoideum cDNA clone:ddv27b14, 3' ...   143   1e-29  1
(BJ429269) Dictyostelium discoideum cDNA clone:ddv2l19, 3' e...   139   2e-28  1
(BJ377627) Dictyostelium discoideum cDNA clone:ddc25i06, 3' ...   139   2e-28  1
(BJ340244) Dictyostelium discoideum cDNA clone:dda12d08, 3' ...   139   2e-28  1
(BJ428773) Dictyostelium discoideum cDNA clone:ddv1o01, 3' e...   135   1e-27  2
(BJ436117) Dictyostelium discoideum cDNA clone:ddv30d02, 3' ...   135   2e-27  1
(BJ433271) Dictyostelium discoideum cDNA clone:ddv21e11, 3' ...   135   2e-27  1
(BJ373172) Dictyostelium discoideum cDNA clone:ddc1e20, 3' e...   133   1e-26  1
(BJ376377) Dictyostelium discoideum cDNA clone:ddc28d08, 3' ...   131   4e-26  1
(BJ378047) Dictyostelium discoideum cDNA clone:ddc26l19, 3' ...   123   9e-24  1
(BJ428358) Dictyostelium discoideum cDNA clone:ddv11l14, 3' ...   107   6e-19  1
(BJ427189) Dictyostelium discoideum cDNA clone:ddv61i15, 5' ...   103   9e-18  1
(AU037532) Dictyostelium discoideum slug cDNA, clone SSD755.      100   1e-16  1
(BJ430424) Dictyostelium discoideum cDNA clone:ddv7p01, 3' e...    72   3e-08  1
(BJ435691) Dictyostelium discoideum cDNA clone:ddv27m22, 3' ...    70   1e-07  1
(BJ435266) Dictyostelium discoideum cDNA clone:ddv26h18, 3' ...    68   5e-07  1
(BJ433577) Dictyostelium discoideum cDNA clone:ddv22f11, 3' ...    66   2e-06  1
(BJ401629) Dictyostelium discoideum cDNA clone:dds23k23, 3' ...    64   7e-06  1
(BJ400339) Dictyostelium discoideum cDNA clone:dds9f22, 3' e...    62   3e-05  1
(BJ344593) Dictyostelium discoideum cDNA clone:dda19i19, 3' ...    62   3e-05  1
(BJ387024) Dictyostelium discoideum cDNA clone:dds11o23, 5' ...    56   0.002  1
(BJ337355) Dictyostelium discoideum cDNA clone:dda57k04, 5' ...    56   0.002  1
(BJ436020) Dictyostelium discoideum cDNA clone:ddv29a18, 3' ...    52   0.028  1
(EI511144) MUGQ_CH252P229L05Sp6_CN396_021 CHORI-252 Vervet M...    50   0.11   1
(BJ382177) Dictyostelium discoideum cDNA clone:ddc43k03, 3' ...    50   0.11   1
(AC232761) Rhesus Macaque Y BAC CH250-116D13 (Children's Hos...    46   1.7    1
(AY760899) RP41_87B13 TJ Papio anubis genomic DNA Papio anub...    46   1.7    1
(FL853904) CCGI2986.b1 CCGI Panicum virgatum etiolated seedl...    46   1.7    1
(FL806806) CCGG6331.g1 CCGG Panicum virgatum late flowering ...    46   1.7    1
(FL692363) CCGA2651.b1 CCGA Panicum virgatum apex + stem (H)...    46   1.7    1
(AC011267) Homo sapiens clone RP11-5A19, LOW-PASS SEQUENCE S...    34   3.4    2
(AL355112) Human chromosome 14 DNA sequence BAC R-159D23 of ...    34   3.4    2
(CA773841) im57h04.y1 HR85 islet Homo sapiens cDNA clone IMA...    34   4.6    2
(AL698358) Homo sapiens mRNA; EST DKFZp686C07108_r1  (from c...    34   4.6    2
(CU655964) Zebrafish DNA sequence from clone CH1073-443F11 i...    44   6.9    1
(AC166097) Mus musculus BAC clone RP24-447P10 from chromosom...    44   6.9    1
(AC189549) Brassica rapa subsp. pekinensis clone KBrH005C21,...    44   6.9    1
(AL049647) Human DNA sequence from clone RP5-1027G4 on chrom...    44   6.9    1
(AC115032) Mus musculus chromosome 9 clone RP24-259M9 map 9,...    44   6.9    1
(FP236418) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   6.9    1
(AZ065663) RPCI-23-433I3.TJ RPCI-23 Mus musculus genomic clo...    44   6.9    1
(CZ887720) fbnb004e011f12f0 Brassica napus methylation filte...    44   6.9    1
(DB254749) Homo sapiens cDNA clone UTERU2009815, 5' end, mRN...    44   6.9    1
(CJ193406) Mus musculus nullipotent stem cell CRL-2070 NE cD...    44   6.9    1
(BX478370) Homo sapiens mRNA; EST DKFZp686D03204_r1  (from c...    44   6.9    1
(GD023664) CCHZ11246.g1 CCHZ Panicum virgatum abiotic and bi...    44   6.9    1
(GD023663) CCHZ11246.b1 CCHZ Panicum virgatum abiotic and bi...    44   6.9    1
(FL939519) CCGP10102.g1 CCGP Panicum virgatum root (L) Panic...    44   6.9    1
(FL939518) CCGP10102.b1 CCGP Panicum virgatum root (L) Panic...    44   6.9    1
(FL908055) CCGO4922.g1 CCGO Panicum virgatum root (H) Panicu...    44   6.9    1
(FL892093) CCGN10179.g1 CCGN Panicum virgatum etiolated seed...    44   6.9    1
(FL892092) CCGN10179.b1 CCGN Panicum virgatum etiolated seed...    44   6.9    1
(FL782296) CCGF11686.g1 CCGF Panicum virgatum early floral b...    44   6.9    1
(FL782295) CCGF11686.b1 CCGF Panicum virgatum early floral b...    44   6.9    1
(FL700376) CCGA6776.g1 CCGA Panicum virgatum apex + stem (H)...    44   6.9    1
(FL698837) CCGA6010.g1 CCGA Panicum virgatum apex + stem (H)...    44   6.9    1
(FL698138) CCGA5654.g1 CCGA Panicum virgatum apex + stem (H)...    44   6.9    1
(FE651375) CBYZ9167.g1 CBYZ Panicum virgatum Kanlow 4 day ol...    44   6.9    1
(FE651374) CBYZ9167.b1 CBYZ Panicum virgatum Kanlow 4 day ol...    44   6.9    1

>(AU060949) Dictyostelium discoideum slug cDNA, clone SLC352.
          Length = 646

 Score =  458 bits (231), Expect(3) = e-150
 Identities = 231/231 (100%)
 Strand = Plus / Plus


Query: 448 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 507
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 192 tatcaataaattaaagaatgatgtaaagggagtcgcagagaacgttagtaatgtagtctc 251


Query: 508 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 567
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 252 atcaaagattgaagcaaccacatcatcagcaaaggccaccattaatgatattaaggatgg 311


Query: 568 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 627
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 312 cgtcaaacaagttgtatctggtaattctggttgtccatactctatgaatggtgaattctc 371


Query: 628 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 678
           |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 372 aatgtgtagccaagtttgcaaagtcagcaaagtagttcaaaagaatccatc 422

 Score =  103 bits (52), Expect(3) = e-150
 Identities = 52/52 (100%)
 Strand = Plus / Plus


Query: 289 cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacaca 340
           ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 33  cttttggtttattgttttaaaatcaatcacataattaaaaatggaagacaca 84

 Score = 32.2 bits (16), Expect(3) = e-150
 Identities = 16/16 (100%)
 Strand = Plus / Plus


Query: 257 cacactcagcatttac 272
           ||||||||||||||||
Sbjct: 1   cacactcagcatttac 16

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 571,359,094
Number of extensions: 34383316
Number of successful extensions: 2560899
Number of sequences better than 10.0: 150
Length of query: 678
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 654
Effective length of database: 99,340,224,878
Effective search space: 64968507070212
Effective search space used: 64968507070212
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.28
Homology vs Protein 
Query= Contig-U16543-1 (Contig-U16543-1Q) /CSM_Contig/Contig-U16543-1Q.Seq.d
         (678 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000114_656(CP000114|pid:none) Streptococcus agalactiae A909, c...    38   0.32
CP001291_394(CP001291|pid:none) Cyanothece sp. PCC 7424, complet...    35   2.1
X92946_23(X92946|pid:none) Lactococcus lactis sp. lactis plasmid...    34   4.6
CP000141_815(CP000141|pid:none) Carboxydothermus hydrogenoforman...    34   4.6

>CP000114_656(CP000114|pid:none) Streptococcus agalactiae A909,
           complete genome.
          Length = 1039

 Score = 37.7 bits (86), Expect = 0.32
 Identities = 18/44 (40%), Positives = 27/44 (61%)
 Frame = +2

Query: 458 LKNDVKGVAENVSNVVSSKIEATTSSAKATINDIKDGVKQVVSG 589
           L N +KGV  NV N + S I +  SS  +T++ I +G+K  +SG
Sbjct: 819 LLNSIKGVVSNVWNGIKSTISSILSSIGSTVSSIWNGMKATISG 862

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 622,987,332
Number of extensions: 7923466
Number of successful extensions: 17203
Number of sequences better than 10.0: 4
Number of HSP's gapped: 17203
Number of HSP's successfully gapped: 4
Length of query: 226
Length of database: 1,061,185,681
Length adjustment: 124
Effective length of query: 102
Effective length of database: 655,898,129
Effective search space: 66901609158
Effective search space used: 66901609158
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.03  gvh: 0.17  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.50  mip: 0.00
 nuc: 1.67  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for Contig-U16543-1 is nuc
VS (DIR, S) 23
VH (FL, L) 1
VF (FL, S) 2
AH (FL, L) 8
AF (FL, S) 2
SL (DIR, L) 1
SS (DIR, S) 40
SH (FL, L) 2
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0