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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U16226-1
Contig ID Contig-U16226-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U16226-1 (Contig-U16226-1Q) /CSM_Contig/Contig-U16226-1Q.Seq.d
ACTGTTTGGCCTACTGGTATTTTTTTTCATAGTCATAAAAAACAAAAAAA
AGAGAAAATGTCAGCTTTAGGTAGAAATCTTGGTAATTTTATTCAAACTT
TTTCAAATAAAACTAGTTCAATTAAAACTGTTAATGGTTTAAAACGTGGA
CTTTTACCATGTTGTGGTTCAACCGTTAGACCAATTGCCAAAGATAATCA
TCACCAATCACCAGTCTTAAATGTAAACAGATCATCAATTTCAGGTATTA
GAACTGAATTATTCAATAGCAATGGTTTAATTACTCAATTCCAAGTTAGA
CAATATTGCAGTGAATCAAAATTACAAGTATCACCAACACCAAAAACTTT
ATTATCAGAAATTTGTCAAAGAGAAATCACTGATTTCAAAACCATCTTTG
AAGATAACAATAACGAAGTCGATAGTTTTTTAGAGGAATTTGGTTTCGAA
TTAACCAAAGAAGGTGACCAAGCATTATTAAAGAAAACTTTTGCCGATGG
TACCAACATTGTCATTCGTTTTGAAACCTTGGAACAACCAATCGAAGAAG
ATTTCGATGAACAAGACGAAAACGATCAAAATGAACGTGACGAAGATGAT
GCTGAAGAACAAGAAGAAGACGAAGTCGAAGAAGAGGAAGAAGAACAACA
AGAAGAAGAAGAAGGTGAAAATGATGAAGAATTAACTGAAGGAGCCGAAG
AAGATTCCCACGAACACCCATTCGAAATTGAAGTCACACCAAAAGGTAAC
GCTTCCGGTAAACTCACTTTTGGTTGTTATGCTTCACACGACGGTAACTA
CACTGTCTCTGGTTTCTACAAAGGTGGTTTCGGTGAAACTGTAAACCCAG
TCGATATTGGTGGAACTTCAATGGAATTCCAAGACAATATCTTATTAGTT
TTACAACAATACGGTATCAATGAACGTTTATCTTTCTTCATCCACGATTA
TGTCCACAACAAGAAAATCAATGATTACGTTGAATCATTCGAAGCCTTAA
AAGATTTTGTTTCAAAAGAATAAATTAAATTTTAAATAAAAAAAATAAAA
AAAAAAAAATAAAAAAAAAGATAATAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------
-GGNTTCGGNNAAACTNTAAACCCNGNCGAAATTGGNGGAACTTCAANGG
AANTCCAANACAAAATNTTNTTAGTTTTACAACAAAACGGGAACAANGAA
CGTTTATCNTTNTTCANCCCCGATTANGGCCCCAACAAAAAAAACA
Gap gap included
Contig length 1286
Chromosome number (1..6, M) 5
Chromosome length 5062330
Start point 1129980
End point 1131165
Strand (PLUS/MINUS) PLUS
Number of clones 10
Number of EST 14
Link to clone list U16226
List of clone(s) 

est1=VFL352F,1,609
est2=FC-AW06F,37,569
est3=VSJ449F,43,608
est4=VFJ605F,134,538
est5=FC-BG07Z,452,1078
est6=VSB674Z,485,1078
est7=CHK474Z,489,1025
est8=VFJ605Z,502,1021
est9=VSE558Z,520,1141
est10=FC-AW06Z,554,1077
est11=VSI215F,591,1089
est12=VSF150F,597,1057
est13=VFL352Z,630,982
est14=VSI215Z,1142,1286
Translated Amino Acid sequence 
claywyffs*s*ktkkrenvsfr*ksw*fysnffk*n*fn*nc*wfktwtftmlwfnr*t
ncqr*sspitslkckqiinfry*n*iiq*qwfnysips*tilq*ikitsitntknfiirn
lskrnh*fqnhl*r*q*rsr*ffrgiwfrinqrr*psiikenfcrwyqhchsf*nlgttn
rrrfr*trrkrsk*t*rr*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpi
rn*shtkr*rfr*thfwllcftrr*lhclwflqrwfr*nckpsrywwnfngiprqylisf
ttiryq*tfiflhprlcpqqenq*lr*iirslkrfcfkrin*ILNKKNKKKKIKKKIIKK
KKKKKKKKKKKKKKKKKKK---

---GFGXTXNPXEIGGTSXEXQXKXXLVLQQNGNXERLSXFXPDXGPNKKN
Translated Amino Acid sequence (All Frames) 
Frame A:
tvwptgiffhshkkqkkekmsalgrnlgnfiqtfsnktssiktvnglkrgllpccgstvr
piakdnhhqspvlnvnrssisgirtelfnsnglitqfqvrqycsesklqvsptpktllse
icqreitdfktifednnnevdsfleefgfeltkegdqallkktfadgtnivirfetleqp
ieedfdeqdendqnerdeddaeeqeedeveeeeeeqqeeeegendeeltegaeedshehp
feievtpkgnasgkltfgcyashdgnytvsgfykggfgetvnpvdiggtsmefqdnillv
lqqyginerlsffihdyvhnkkindyvesfealkdfvske*ikf*ikkikkkk*kkr**k
kkkkkkkkkkkkkkkkkkkk---

---GFGXTXNPXEIGGTSXEXQXKXXLVLQQNGNXERLSXFXPDXGPNKKN

Frame B:
lfgllvfffiviknkkkrkcql*veilvilfklfqiklvqlkllmv*nvdfyhvvvqpld
qlpkiiitnhqs*m*tdhqfqvlelnysiamv*llnskldniavnqnykyhqhqklyyqk
fvkeksliskpslkititksivf*rnlvsn*pkkvtkhy*rkllpmvptlsfvlkpwnnq
skkismnktktikmnvtkmmlknkkktkskkrkknnkkkkkvkmmkn*lkepkkiptnth
sklkshqkvtlpvnsllvvmlhttvttlslvstkvvsvkl*tqsilvelqwnsktisy*f
ynntvsmnvylssstimsttrksmitlnhskp*kilfqknklnfk*kk*kkknkkkdnkk
kkkkkkkkkkkkkkkkkkkk---

---xsxkl*txxklxelqxxsxtkxx*fynktgtxnvyxxsxpixaptkkt

Frame C:
claywyffs*s*ktkkrenvsfr*ksw*fysnffk*n*fn*nc*wfktwtftmlwfnr*t
ncqr*sspitslkckqiinfry*n*iiq*qwfnysips*tilq*ikitsitntknfiirn
lskrnh*fqnhl*r*q*rsr*ffrgiwfrinqrr*psiikenfcrwyqhchsf*nlgttn
rrrfr*trrkrsk*t*rr*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpi
rn*shtkr*rfr*thfwllcftrr*lhclwflqrwfr*nckpsrywwnfngiprqylisf
ttiryq*tfiflhprlcpqqenq*lr*iirslkrfcfkrin*ILNKKNKKKKIKKKIIKK
KKKKKKKKKKKKKKKKKKK---

---xrxnxkpxrnwxnfxgxpxqnxxsfttkreqxtfixxxprlxpqqkk
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U16226-1 (Contig-U16226-1Q)
/CSM_Contig/Contig-U16226-1Q.Seq.d
         (1296 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U16226-1 (Contig-U16226-1Q) /CSM_Contig/Conti...  1092  0.0
Contig-U08935-1 (Contig-U08935-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U15728-1 (Contig-U15728-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U14248-1 (Contig-U14248-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U16452-1 (Contig-U16452-1Q) /CSM_Contig/Conti...    40  0.008
Contig-U16170-1 (Contig-U16170-1Q) /CSM_Contig/Conti...    40  0.008
Contig-U15746-1 (Contig-U15746-1Q) /CSM_Contig/Conti...    40  0.008
Contig-U16299-1 (Contig-U16299-1Q) /CSM_Contig/Conti...    38  0.033
Contig-U02891-1 (Contig-U02891-1Q) /CSM_Contig/Conti...    36  0.13
Contig-U12108-1 (Contig-U12108-1Q) /CSM_Contig/Conti...    34  0.52

>Contig-U16226-1 (Contig-U16226-1Q) /CSM_Contig/Contig-U16226-1Q.Seq.d
          Length = 1296

 Score = 1092 bits (551), Expect = 0.0
 Identities = 551/551 (100%)
 Strand = Plus / Plus

                                                                       
Query: 52  gagaaaatgtcagctttaggtagaaatcttggtaattttattcaaactttttcaaataaa 111
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 52  gagaaaatgtcagctttaggtagaaatcttggtaattttattcaaactttttcaaataaa 111

                                                                       
Query: 112 actagttcaattaaaactgttaatggtttaaaacgtggacttttaccatgttgtggttca 171
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 112 actagttcaattaaaactgttaatggtttaaaacgtggacttttaccatgttgtggttca 171

                                                                       
Query: 172 accgttagaccaattgccaaagataatcatcaccaatcaccagtcttaaatgtaaacaga 231
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 172 accgttagaccaattgccaaagataatcatcaccaatcaccagtcttaaatgtaaacaga 231

                                                                       
Query: 232 tcatcaatttcaggtattagaactgaattattcaatagcaatggtttaattactcaattc 291
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 232 tcatcaatttcaggtattagaactgaattattcaatagcaatggtttaattactcaattc 291

                                                                       
Query: 292 caagttagacaatattgcagtgaatcaaaattacaagtatcaccaacaccaaaaacttta 351
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 292 caagttagacaatattgcagtgaatcaaaattacaagtatcaccaacaccaaaaacttta 351

                                                                       
Query: 352 ttatcagaaatttgtcaaagagaaatcactgatttcaaaaccatctttgaagataacaat 411
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 352 ttatcagaaatttgtcaaagagaaatcactgatttcaaaaccatctttgaagataacaat 411

                                                                       
Query: 412 aacgaagtcgatagttttttagaggaatttggtttcgaattaaccaaagaaggtgaccaa 471
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 412 aacgaagtcgatagttttttagaggaatttggtttcgaattaaccaaagaaggtgaccaa 471

                                                                       
Query: 472 gcattattaaagaaaacttttgccgatggtaccaacattgtcattcgttttgaaaccttg 531
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 472 gcattattaaagaaaacttttgccgatggtaccaacattgtcattcgttttgaaaccttg 531

                                                                       
Query: 532 gaacaaccaatcgaagaagatttcgatgaacaagacgaaaacgatcaaaatgaacgtgac 591
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 532 gaacaaccaatcgaagaagatttcgatgaacaagacgaaaacgatcaaaatgaacgtgac 591

                      
Query: 592 gaagatgatgc 602
           |||||||||||
Sbjct: 592 gaagatgatgc 602


 Score =  706 bits (356), Expect = 0.0
 Identities = 356/356 (100%)
 Strand = Plus / Plus

                                                                        
Query: 682  ttaactgaaggagccgaagaagattcccacgaacacccattcgaaattgaagtcacacca 741
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 682  ttaactgaaggagccgaagaagattcccacgaacacccattcgaaattgaagtcacacca 741

                                                                        
Query: 742  aaaggtaacgcttccggtaaactcacttttggttgttatgcttcacacgacggtaactac 801
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 742  aaaggtaacgcttccggtaaactcacttttggttgttatgcttcacacgacggtaactac 801

                                                                        
Query: 802  actgtctctggtttctacaaaggtggtttcggtgaaactgtaaacccagtcgatattggt 861
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 802  actgtctctggtttctacaaaggtggtttcggtgaaactgtaaacccagtcgatattggt 861

                                                                        
Query: 862  ggaacttcaatggaattccaagacaatatcttattagttttacaacaatacggtatcaat 921
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 862  ggaacttcaatggaattccaagacaatatcttattagttttacaacaatacggtatcaat 921

                                                                        
Query: 922  gaacgtttatctttcttcatccacgattatgtccacaacaagaaaatcaatgattacgtt 981
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 922  gaacgtttatctttcttcatccacgattatgtccacaacaagaaaatcaatgattacgtt 981

                                                                    
Query: 982  gaatcattcgaagccttaaaagattttgtttcaaaagaataaattaaattttaaat 1037
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 982  gaatcattcgaagccttaaaagattttgtttcaaaagaataaattaaattttaaat 1037


 Score =  167 bits (84), Expect = 5e-41
 Identities = 132/132 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1155 ttcggnnaaactntaaacccngncgaaattggnggaacttcaanggaantccaanacaaa 1214
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1155 ttcggnnaaactntaaacccngncgaaattggnggaacttcaanggaantccaanacaaa 1214

                                                                        
Query: 1215 atnttnttagttttacaacaaaacgggaacaangaacgtttatcnttnttcanccccgat 1274
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1215 atnttnttagttttacaacaaaacgggaacaangaacgtttatcnttnttcanccccgat 1274

                        
Query: 1275 tanggccccaac 1286
            ||||||||||||
Sbjct: 1275 tanggccccaac 1286


 Score =  109 bits (55), Expect = 1e-23
 Identities = 114/139 (82%)
 Strand = Plus / Plus

                                                                        
Query: 829  ttcggtgaaactgtaaacccagtcgatattggtggaacttcaatggaattccaagacaat 888
            |||||  ||||| ||||||| | ||| ||||| |||||||||| |||| ||||| |||| 
Sbjct: 1155 ttcggnnaaactntaaacccngncgaaattggnggaacttcaanggaantccaanacaaa 1214

                                                                        
Query: 889  atcttattagttttacaacaatacggtatcaatgaacgtttatctttcttcatccacgat 948
            || || ||||||||||||||| |||| | ||| ||||||||||| || |||| || ||||
Sbjct: 1215 atnttnttagttttacaacaaaacgggaacaangaacgtttatcnttnttcanccccgat 1274

                               
Query: 949  tatgtccacaacaagaaaa 967
            || | || |||||| ||||
Sbjct: 1275 tanggccccaacaaaaaaa 1293


 Score =  105 bits (53), Expect = 2e-22
 Identities = 101/122 (82%)
 Strand = Plus / Plus

                                                                        
Query: 1155 ttcggnnaaactntaaacccngncgaaattggnggaacttcaanggaantccaanacaaa 1214
            |||||  ||||| ||||||| | ||| ||||| |||||||||| |||| ||||| |||| 
Sbjct: 829  ttcggtgaaactgtaaacccagtcgatattggtggaacttcaatggaattccaagacaat 888

                                                                        
Query: 1215 atnttnttagttttacaacaaaacgggaacaangaacgtttatcnttnttcanccccgat 1274
            || || ||||||||||||||| |||| | ||| ||||||||||| || |||| || ||||
Sbjct: 889  atcttattagttttacaacaatacggtatcaatgaacgtttatctttcttcatccacgat 948

              
Query: 1275 ta 1276
            ||
Sbjct: 949  ta 950


 Score = 71.9 bits (36), Expect = 2e-12
 Identities = 36/36 (100%)
 Strand = Plus / Plus

                                              
Query: 1  actgtttggcctactggtattttttttcatagtcat 36
          ||||||||||||||||||||||||||||||||||||
Sbjct: 1  actgtttggcctactggtattttttttcatagtcat 36


>Contig-U08935-1 (Contig-U08935-1Q) /CSM_Contig/Contig-U08935-1Q.Seq.d
          Length = 1165

 Score = 44.1 bits (22), Expect = 5e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 324 acaagtatcaccaacaccaaaa 345
           ||||||||||||||||||||||
Sbjct: 705 acaagtatcaccaacaccaaaa 726


 Score = 30.2 bits (15), Expect = 8.1
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                          
Query: 332 caccaacaccaaaaa 346
           |||||||||||||||
Sbjct: 229 caccaacaccaaaaa 243


>Contig-U15728-1 (Contig-U15728-1Q) /CSM_Contig/Contig-U15728-1Q.Seq.d
          Length = 2152

 Score = 42.1 bits (21), Expect = 0.002
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 6  ttggcctactggtattttttt 26
          |||||||||||||||||||||
Sbjct: 5  ttggcctactggtattttttt 25


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 18,206
Number of Sequences: 8402
Number of extensions: 18206
Number of successful extensions: 2130
Number of sequences better than 10.0: 301
length of query: 1296
length of database: 8,075,542
effective HSP length: 16
effective length of query: 1280
effective length of database: 7,941,110
effective search space: 10164620800
effective search space used: 10164620800
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 3.22
Homology vs DNA 
Query= Contig-U16226-1 (Contig-U16226-1Q) /CSM_Contig/Contig-U16226-1Q.Seq.d
         (1296 letters)

Database: ddbj_B
           98,226,423 sequences; 98,766,808,389 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU269936) Dictyostelium discoideum vegetative cDNA clone:VS...  1055   0.0    2
(C25721) Dictyostelium discoideum gamete cDNA, clone FC-AW06.    1027   0.0    1
(BJ415744) Dictyostelium discoideum cDNA clone:ddv23h13, 5' ...  1003   0.0    2
(AU284653) Dictyostelium discoideum gamete cDNA clone:FC-BG0...   597   0.0    2
(AU262173) Dictyostelium discoideum vegetative cDNA clone:VS...   613   0.0    2
(BJ414684) Dictyostelium discoideum cDNA clone:ddv20i02, 5' ...   785   0.0    1
(BJ381823) Dictyostelium discoideum cDNA clone:ddc42d20, 3' ...   547   0.0    3
(AU263198) Dictyostelium discoideum vegetative cDNA clone:VS...   599   0.0    2
(BJ432868) Dictyostelium discoideum cDNA clone:ddv20i02, 3' ...   505   0.0    3
(C25722) Dictyostelium discoideum gamete cDNA, clone FC-AW06.     597   0.0    2
(AU268332) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(AU264783) Dictyostelium discoideum vegetative cDNA clone:VS...   613   e-171  1
(BJ433999) Dictyostelium discoideum cDNA clone:ddv23h13, 3' ...   325   2e-84  1
(AU268333) Dictyostelium discoideum vegetative cDNA clone:VS...   167   1e-36  1
(AU264784) Dictyostelium discoideum vegetative cDNA clone:VS...    56   0.003  1
(DJ208950) Method for identification of useful proteins deri...    40   0.28   2
(X06077) Yeast ARO1 gene for arom multifunctional enzyme.          40   0.35   2
(EA376499) Sequence 25322 from patent US 7314974.                  40   0.35   2
(AP006627) Bacillus clausii KSM-K16 DNA, complete genome.          48   0.83   1
(AC179380) Strongylocentrotus purpuratus clone R3-59O6, WORK...    32   1.7    2
(Z48179) S.cerevisiae chromosome IV cosmid 9302.                   40   2.3    2
(CR339055) Zebrafish DNA sequence from clone CH211-67C24 in ...    46   3.3    1
(AL805920) Mouse DNA sequence from clone RP23-162M7 on chrom...    46   3.3    1
(AM465684) Vitis vinifera contig VV78X155772.10, whole genom...    46   3.3    1
(AC109474) Homo sapiens chromosome 5 clone RP11-414I19, comp...    46   3.3    1
(AC092742) Mus musculus strain C57BL6/J clone RP23-158O21, W...    46   3.3    1
(AC034163) Homo sapiens chromosome 5 clone RP11-414I19, WORK...    46   3.3    1
(ER396128) 1095344024794 Global-Ocean-Sampling_GS-34-01-01-1...    46   3.3    1
(ER394955) 1095339049699 Global-Ocean-Sampling_GS-34-01-01-1...    46   3.3    1
(ER336044) 1092344306779 Global-Ocean-Sampling_GS-34-01-01-1...    46   3.3    1
(EJ733308) 1092961012048 Global-Ocean-Sampling_GS-30-02-01-1...    46   3.3    1
(EJ568461) 1092960012253 Global-Ocean-Sampling_GS-29-01-01-1...    46   3.3    1
(EJ214961) 1092351056394 Global-Ocean-Sampling_GS-27-01-01-1...    46   3.3    1
(DX543587) GH_MBb0036A19r GH_MBb Gossypium hirsutum genomic ...    46   3.3    1
(ES451266) 26236 Myzus persicae 2001-12 (red), Fenton Myzus ...    46   3.3    1
(AC201653) Strongylocentrotus purpuratus clone R3-1015L11, W...    44   5.8    4
(AC098708) Mus musculus BAC clone RP23-1I16 from 13, complet...    34   6.0    2
(AC109831) Mus musculus BAC clone RP24-104B17 from chromosom...    34   6.2    2
(AC155308) Mus musculus BAC clone RP24-150P15 from chromosom...    34   6.2    2
(AZ620377) 1M0453O06F Mouse 10kb plasmid UUGC1M library Mus ...    34   8.5    2
(FD934442) RS1FW67JQ RS1(AR) Raphanus sativus var. oleiformi...    38   8.8    2
(AC007529) Homo sapiens 12p13 BAC RPCI11-69C13 (Roswell Park...    40   9.4    3
(U56961) Caenorhabditis elegans cosmid T19D7, complete seque...    36   9.4    3

>(AU269936) Dictyostelium discoideum vegetative cDNA clone:VSJ449,
           5' end single read.
          Length = 566

 Score = 1055 bits (532), Expect(2) = 0.0
 Identities = 532/532 (100%)
 Strand = Plus / Plus


Query: 52  gagaaaatgtcagctttaggtagaaatcttggtaattttattcaaactttttcaaataaa 111
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 10  gagaaaatgtcagctttaggtagaaatcttggtaattttattcaaactttttcaaataaa 69


Query: 112 actagttcaattaaaactgttaatggtttaaaacgtggacttttaccatgttgtggttca 171
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 70  actagttcaattaaaactgttaatggtttaaaacgtggacttttaccatgttgtggttca 129


Query: 172 accgttagaccaattgccaaagataatcatcaccaatcaccagtcttaaatgtaaacaga 231
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 130 accgttagaccaattgccaaagataatcatcaccaatcaccagtcttaaatgtaaacaga 189


Query: 232 tcatcaatttcaggtattagaactgaattattcaatagcaatggtttaattactcaattc 291
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 190 tcatcaatttcaggtattagaactgaattattcaatagcaatggtttaattactcaattc 249


Query: 292 caagttagacaatattgcagtgaatcaaaattacaagtatcaccaacaccaaaaacttta 351
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 250 caagttagacaatattgcagtgaatcaaaattacaagtatcaccaacaccaaaaacttta 309


Query: 352 ttatcagaaatttgtcaaagagaaatcactgatttcaaaaccatctttgaagataacaat 411
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 310 ttatcagaaatttgtcaaagagaaatcactgatttcaaaaccatctttgaagataacaat 369


Query: 412 aacgaagtcgatagttttttagaggaatttggtttcgaattaaccaaagaaggtgaccaa 471
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 370 aacgaagtcgatagttttttagaggaatttggtttcgaattaaccaaagaaggtgaccaa 429


Query: 472 gcattattaaagaaaacttttgccgatggtaccaacattgtcattcgttttgaaaccttg 531
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 430 gcattattaaagaaaacttttgccgatggtaccaacattgtcattcgttttgaaaccttg 489


Query: 532 gaacaaccaatcgaagaagatttcgatgaacaagacgaaaacgatcaaaatg 583
           ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 490 gaacaaccaatcgaagaagatttcgatgaacaagacgaaaacgatcaaaatg 541

 Score = 34.2 bits (17), Expect(2) = 0.0
 Identities = 20/21 (95%)
 Strand = Plus / Plus


Query: 583 gaacgtgacgaagatgatgct 603
           |||||||||||| ||||||||
Sbjct: 542 gaacgtgacgaaaatgatgct 562

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 98226423
Number of Hits to DB: 1,046,028,551
Number of extensions: 57713119
Number of successful extensions: 4394221
Number of sequences better than 10.0: 43
Length of query: 1296
Length of database: 98,766,808,389
Length adjustment: 24
Effective length of query: 1272
Effective length of database: 96,409,374,237
Effective search space: 122632724029464
Effective search space used: 122632724029464
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.27
Homology vs Protein 
Query= Contig-U16226-1 (Contig-U16226-1Q) /CSM_Contig/Contig-U16226-1Q.Seq.d
         (1296 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY086223_1(AY086223|pid:none) Arabidopsis thaliana clone 22656 m...    40   0.18
AP004702_16(AP004702|pid:none) Oryza sativa Japonica Group genom...    36   2.7
CP000721_1484(CP000721|pid:none) Clostridium beijerinckii NCIMB ...    36   2.7
(P40513) RecName: Full=Mitochondrial acidic protein MAM33; Flags...    35   4.6
CR380959_553(CR380959|pid:none) Candida glabrata strain CBS138 c...    35   4.6
AY557860_1(AY557860|pid:none) Saccharomyces cerevisiae clone FLH...    35   4.6
CP001348_1465(CP001348|pid:none) Clostridium cellulolyticum H10,...    35   5.9

>AY086223_1(AY086223|pid:none) Arabidopsis thaliana clone 22656 mRNA,
            complete sequence.
          Length = 240

 Score = 40.0 bits (92), Expect = 0.18
 Identities = 21/65 (32%), Positives = 34/65 (52%), Gaps = 5/65 (7%)
 Frame = +1

Query: 832  GETVNPVDIGGTSME-----FQDNILLVLQQYGINERLSFFIHDYVHNKKINDYVESFEA 996
            G   +P   GG   E      QD +   L++ GI++ L+ F+H Y+ NK   +YV   E+
Sbjct: 173  GINTSPSSYGGPEFEELDDQLQDALYQFLEERGISDELAVFLHRYMKNKGKAEYVRWMES 232

Query: 997  LKDFV 1011
            +K +V
Sbjct: 233  VKSYV 237

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 1,535,427,120
Number of extensions: 27249696
Number of successful extensions: 63387
Number of sequences better than 10.0: 7
Number of HSP's gapped: 63355
Number of HSP's successfully gapped: 7
Length of query: 432
Length of database: 1,061,185,681
Length adjustment: 132
Effective length of query: 300
Effective length of database: 629,750,545
Effective search space: 188925163500
Effective search space used: 188925163500
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.40  gvh: 0.32  alm: 0.63  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 2.42  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for Contig-U16226-1 is nuc
VS (DIR, S) 5
VH (FL, L) 0
VF (FL, S) 2
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 2
FC-IC (SUB) 0