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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U16221-1
Contig ID Contig-U16221-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U16221-1 (Contig-U16221-1Q) /CSM_Contig/Contig-U16221-1Q.Seq.d
AATTATGTTGNAACANAAAATTGTTATGTTCCANCAATACATTAGTTCAA
TTACCCAACAATTCCAACNACTTCNACTTCAACCACAAAATANCCAAACT
CAACTTCAACTCCAACAACTCCAACAATTTACAAGGAACATTATACTCAA
CAATGTATGTTGCATCAACAAAAATTAATGCAAATTCAATATCAATACAC
ACAATTCCAACCAATCCAAACACCACAATCTCAACAATCCCAAATCCAAC
TTTTCCAACATATGCTCCAACAACAACAAGCTCAACAACAACAACAACAA
GCTCAACAACAAGTTCAACAAGTCCAACAAGTCCAACAACAACAAAACTA
TTATCTTCAACAACAACAAGCACTCTTACTTCAACAACAACAACAAGCAC
TTTTACACCAACAACAACAACAGCAACAACAACAAGTAGAACAATCCCAA
CCAGAAGAACCATTAGAACAACAAACTGAAGAAATCAACATAGAAGAGGA
CGAAAATACAGTTGAGCAACCACAACAACCAACCACTGAACAACAAACTC
AAGTTGAACAACAAACAACCTCAACCACCACCTACCAACAACAACAACAA
GATAATGAAGATGAAGGTGTTTGGACTACATCAATTAAACAAAATAACCA
AGAAAATAAAACAACCACAACTGCTCACCTTAGCGTCCAAACTGAAGAAT
TAAAAGCTCCAATTGATATTGAAGAGGAACAAGAAGAAGCTTCCATTCAA
AATGCTATTGAAGAAATTAATAATAACACCATACAAACCACTACCACTAA
CAACACATCTACCACTACCATCACCAATAATAACACCACTGCAACCACAA
CTACAACCACCACAACAGAAACAAATATCATTTCAGCCGCAAATCCATGG
GCTCCATCAGAATCAGTTGTTCCAAAGAAGAAATCATTAGCAGAGATTCA
AGCAGAGGAAAGAGAGCAAAAGAAGAGAGAGATGGAGGAGGCCAAAATTA
GAAACATTGAAAATAGCAAAAATCATGGTTCTAGCAATGTTATGAAATGG
GCAGGTGCAGGCACACCAACTTGGGTCAGTAGATCTTGGCGTTAAATCAT
TATCATTAAAAGATATCCAAGAGGAAGAGAGAGCTCGTGAAAAGAAAGAA
CAACCAATCGTTGATAATGCATCTGACTTTGAAAGAAAGAAACCAGTATC
ACTCTCTGATGTCATGAGAGAACAAGCCAAAGAAAAGGAAAAGAAACAAC
AACAAGAACAATTAGCACAACAACAACAACAAGCTCAACAAGAACATCAA
AAATCAACTTTCAACGCCTCATCACCATGGTCAATTGATGAAAGAAAACC
AAACAACAACAACAACCATTCTTCATATTCACCAATTTCAATCAATAACA
ACAACAATAACAACATTAACAACGTATCACAAC----------CAGTCAA
TAAGATGACAAGCAGACCAACTGATGATTTCTGGTCAGAACATACCACCG
CCAAACCATCACTCTCTGTTAGAAGTAATGCACCAGTTCAACAACAAGTC
GGTTCATTCAACGAATATCCAACCTTAAACATTTTACCAAATAGACAAGC
CACCATCGTTAGTAAGCATACACCAGTTTCAACCTTGAATAAAAAACCAA
CAACCACAACCACCGTACCAGGTCAAGCTAGACCAGATTTCATCAAATGG
TGTCATCAACAATTGAAGGCATTAACTAGCAATGATGTCTCTGTTATCAC
TGAACTCCTTTGTTCAATGAAAACTGAAAGTGAAATTCGTGAATGCGCTC
AAGAATGTCTTGGTTATAGTTCAGAGGTCAATATCTTTTTGAATGAATAT
CTTTTAGCAAGAAGTGATGAACCAGGTTTGGCTTTTGAAAGTTCAAGTCC
AGTCATCACAATTCCAGCAAAGAAAACAAATAAATCATCTCAAACAAATC
CATCAAAAACTAAAAAGAAGAAATAAATTCAAACATTACCATTACTAACA
CAACAAAATAAAATAATAATAATAATAAAAAAAAAAATTTCAAATATTAC
ATCATATATCAATATAATCTATAATATCAAATAAATAATAAAAAAAAAAA
AAATAAAAAAAAAAAAATATGAATTAAATT
Gap gap included
Contig length 2120
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 1883650
End point 1881531
Strand (PLUS/MINUS) MINUS
Number of clones 10
Number of EST 12
Link to clone list U16221
List of clone(s) 

est1=VHN264Z,-2,602
est2=VHB118Z,98,815
est3=VHC187Z,102,601
est4=AFE109Z,359,605
est5=CHH139Z,424,528
est6=VSJ692E,501,1202
est7=VSF276F,919,1433
est8=VSF344F,919,1368
est9=FC-BI09E,1434,2019
est10=VSF276Z,1511,2056
est11=VSF344Z,1594,2048
est12=VSA162Z,1608,2120
Translated Amino Acid sequence 
imlxxkivmfxqyissitqqfqxlxlqpqnxqtqlqlqqlqqftrniilnnvccinkn*c
kfninthnsnqskhhnlnnpksnfsnicsnnnklnnnnnklnnkfnksnksnnnktiifn
nnkhsyfnnnnkhfytnnnnsnnnk*nnpnqknh*nnklkkst*krtkiqlsnhnnqpln
nklklnnkqpqppptnnnnkimkmkvfglhqlnkitkkikqpqlltlasklkn*klqlil
krnkkklpfkmllkkliitpykplpltthlplpspiitplqpqlqppqqkqisfqpqihg
lhqnqlfqrrnh*qrfkqrkeskrrerwrrpkletlkiakimvlaml*NGQVQAHQLGSV
DLGVKSLSLKDIQEEERAREKKEQPIVDNASDFERKKPVSLSDVMREQAKEKEKKQQQEQ
LAQQQQQAQQEHQKSTFNASSPWSIDERKPNNNNNHSSYSPISINNNNNNNINNVSQ---

---VNKMTSRPTDDFWSEHTTAKPSLSVRSNAPVQQQVGSFNEYPTLNILPNRQATIVSK
HTPVSTLNKKPTTTTTVPGQARPDFIKWCHQQLKALTSNDVSVITELLCSMKTESEIREC
AQECLGYSSEVNIFLNEYLLARSDEPGLAFESSSPVITIPAKKTNKSSQTNPSKTKKKK*
iqtlplltqqnkiiiiikkkisnitsyiniiynik*iikkkkikkkkyeln
Translated Amino Acid sequence (All Frames) 
Frame A:
nyvxtxncyvpxih*fnyptipttststtkxpnststpttptiykehytqqcmlhqqklm
qiqyqytqfqpiqtpqsqqsqiqlfqhmlqqqqaqqqqqqaqqqvqqvqqvqqqqnyylq
qqqalllqqqqqallhqqqqqqqqqveqsqpeepleqqteeinieedentveqpqqptte
qqtqveqqttstttyqqqqqdnedegvwttsikqnnqenkttttahlsvqteelkapidi
eeeqeeasiqnaieeinnntiqttttnntstttitnnnttattttttttetniisaanpw
apsesvvpkkkslaeiqaeereqkkremeeakirniensknhgssnvmkwagagtptwvs
rswr*iiiikryprgress*kerttnr**ci*l*kketsitl*chertsqrkgketttrt
istttttsstrtskinfqrlitmvn**kktkqqqqpffiftnfnq*qqq*qh*qrit---

---qsir*qadqlmisgqnipppnhhsllevmhqfnnksvhstniqp*tfyqidkppslv
sihqfqp*iknqqpqppyqvkldqissngvinn*rh*lammsllslnsfvq*klkvkfvn
alknvlvivqrsisf*mnif*qevmnqvwllkvqvqssqfqqrkqinhlkqihqklkrrn
kfkhyhy*hnkik*****kkkfqilhhisi*siisnk**kkkk*kkknmn*i

Frame B:
imlxxkivmfxqyissitqqfqxlxlqpqnxqtqlqlqqlqqftrniilnnvccinkn*c
kfninthnsnqskhhnlnnpksnfsnicsnnnklnnnnnklnnkfnksnksnnnktiifn
nnkhsyfnnnnkhfytnnnnsnnnk*nnpnqknh*nnklkkst*krtkiqlsnhnnqpln
nklklnnkqpqppptnnnnkimkmkvfglhqlnkitkkikqpqlltlasklkn*klqlil
krnkkklpfkmllkkliitpykplpltthlplpspiitplqpqlqppqqkqisfqpqihg
lhqnqlfqrrnh*qrfkqrkeskrrerwrrpkletlkiakimvlaml*NGQVQAHQLGSV
DLGVKSLSLKDIQEEERAREKKEQPIVDNASDFERKKPVSLSDVMREQAKEKEKKQQQEQ
LAQQQQQAQQEHQKSTFNASSPWSIDERKPNNNNNHSSYSPISINNNNNNNINNVSQ---

---sq*ddkqtn**flvrtyhrqtitlc*k*ctssttsrfiqrisnlkhftk*tshhr**
aytsfnle*ktnnhnhrtrss*trfhqmvsstiegin*q*clcyh*tplfnen*k*ns*m
rsrmswl*frgqylfe*isfskk**trfgf*kfksshhnsskenk*iisnksikn*keei
nsnititnttk*nnnnnkkknfkyyiiyqynl*yqinnkkkknkkkki*ik

Frame C:
lcxnxkllcsxntlvqlpnnsnxfxfnhkixklnfnsnnsnnlqgtlystmyvastkina
nsisihtiptnpnttistipnptfptyaptttsstttttssttsstsptsptttkllsst
ttstltsttttstftpttttatttsrtiptrrtirttn*rnqhrrgrkys*attttnh*t
tnss*ttnnlnhhlpttttr**r*rcldyin*tk*prk*nnhncsp*rpn*rikssn*y*
rgtrrsfhskcy*rn***hhtnhyh*qhiyhyhhq**hhcnhnynhhnrnkyhfsrksmg
siriscskeeiisrdssrgkrakeerdgggqn*kh*k*qkswf*qcyemgrcrhtnlgq*
ilalnhyh*kiskrkrelvkrknnqslimhltlkernqyhslms*enkpkkrkrnnnknn
*hnnnnklnkniknqlstphhhgqlmkenqtttttilhihqfqsitttittlttyhn---

---VNKMTSRPTDDFWSEHTTAKPSLSVRSNAPVQQQVGSFNEYPTLNILPNRQATIVSK
HTPVSTLNKKPTTTTTVPGQARPDFIKWCHQQLKALTSNDVSVITELLCSMKTESEIREC
AQECLGYSSEVNIFLNEYLLARSDEPGLAFESSSPVITIPAKKTNKSSQTNPSKTKKKK*
iqtlplltqqnkiiiiikkkisnitsyiniiynik*iikkkkikkkkyeln
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U16221-1 (Contig-U16221-1Q)
/CSM_Contig/Contig-U16221-1Q.Seq.d
         (2130 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U16221-1 (Contig-U16221-1Q) /CSM_Contig/Conti...   957  0.0
Contig-U15540-1 (Contig-U15540-1Q) /CSM_Contig/Conti...    46  2e-04
Contig-U13933-1 (Contig-U13933-1Q) /CSM_Contig/Conti...    44  9e-04
Contig-U12144-1 (Contig-U12144-1Q) /CSM_Contig/Conti...    44  9e-04
Contig-U11567-1 (Contig-U11567-1Q) /CSM_Contig/Conti...    44  9e-04
Contig-U15517-1 (Contig-U15517-1Q) /CSM_Contig/Conti...    42  0.004
Contig-U15251-1 (Contig-U15251-1Q) /CSM_Contig/Conti...    42  0.004
Contig-U12504-1 (Contig-U12504-1Q) /CSM_Contig/Conti...    42  0.004
Contig-U09331-1 (Contig-U09331-1Q) /CSM_Contig/Conti...    42  0.004
Contig-U15705-1 (Contig-U15705-1Q) /CSM_Contig/Conti...    40  0.014

>Contig-U16221-1 (Contig-U16221-1Q) /CSM_Contig/Contig-U16221-1Q.Seq.d
          Length = 2130

 Score =  957 bits (483), Expect = 0.0
 Identities = 513/513 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1408 taacaacattaacaacgtatcacaacnnnnnnnnnncagtcaataagatgacaagcagac 1467
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1408 taacaacattaacaacgtatcacaacnnnnnnnnnncagtcaataagatgacaagcagac 1467

                                                                        
Query: 1468 caactgatgatttctggtcagaacataccaccgccaaaccatcactctctgttagaagta 1527
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1468 caactgatgatttctggtcagaacataccaccgccaaaccatcactctctgttagaagta 1527

                                                                        
Query: 1528 atgcaccagttcaacaacaagtcggttcattcaacgaatatccaaccttaaacattttac 1587
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1528 atgcaccagttcaacaacaagtcggttcattcaacgaatatccaaccttaaacattttac 1587

                                                                        
Query: 1588 caaatagacaagccaccatcgttagtaagcatacaccagtttcaaccttgaataaaaaac 1647
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1588 caaatagacaagccaccatcgttagtaagcatacaccagtttcaaccttgaataaaaaac 1647

                                                                        
Query: 1648 caacaaccacaaccaccgtaccaggtcaagctagaccagatttcatcaaatggtgtcatc 1707
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1648 caacaaccacaaccaccgtaccaggtcaagctagaccagatttcatcaaatggtgtcatc 1707

                                                                        
Query: 1708 aacaattgaaggcattaactagcaatgatgtctctgttatcactgaactcctttgttcaa 1767
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1708 aacaattgaaggcattaactagcaatgatgtctctgttatcactgaactcctttgttcaa 1767

                                                                        
Query: 1768 tgaaaactgaaagtgaaattcgtgaatgcgctcaagaatgtcttggttatagttcagagg 1827
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1768 tgaaaactgaaagtgaaattcgtgaatgcgctcaagaatgtcttggttatagttcagagg 1827

                                                                        
Query: 1828 tcaatatctttttgaatgaatatcttttagcaagaagtgatgaaccaggtttggcttttg 1887
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1828 tcaatatctttttgaatgaatatcttttagcaagaagtgatgaaccaggtttggcttttg 1887

                                             
Query: 1888 aaagttcaagtccagtcatcacaattccagcaa 1920
            |||||||||||||||||||||||||||||||||
Sbjct: 1888 aaagttcaagtccagtcatcacaattccagcaa 1920


 Score =  670 bits (338), Expect = 0.0
 Identities = 338/338 (100%)
 Strand = Plus / Plus

                                                                       
Query: 601 gataatgaagatgaaggtgtttggactacatcaattaaacaaaataaccaagaaaataaa 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 gataatgaagatgaaggtgtttggactacatcaattaaacaaaataaccaagaaaataaa 660

                                                                       
Query: 661 acaaccacaactgctcaccttagcgtccaaactgaagaattaaaagctccaattgatatt 720
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661 acaaccacaactgctcaccttagcgtccaaactgaagaattaaaagctccaattgatatt 720

                                                                       
Query: 721 gaagaggaacaagaagaagcttccattcaaaatgctattgaagaaattaataataacacc 780
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 721 gaagaggaacaagaagaagcttccattcaaaatgctattgaagaaattaataataacacc 780

                                                                       
Query: 781 atacaaaccactaccactaacaacacatctaccactaccatcaccaataataacaccact 840
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781 atacaaaccactaccactaacaacacatctaccactaccatcaccaataataacaccact 840

                                                                       
Query: 841 gcaaccacaactacaaccaccacaacagaaacaaatatcatttcagccgcaaatccatgg 900
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 841 gcaaccacaactacaaccaccacaacagaaacaaatatcatttcagccgcaaatccatgg 900

                                                 
Query: 901 gctccatcagaatcagttgttccaaagaagaaatcatt 938
           ||||||||||||||||||||||||||||||||||||||
Sbjct: 901 gctccatcagaatcagttgttccaaagaagaaatcatt 938


 Score =  448 bits (226), Expect = e-125
 Identities = 226/226 (100%)
 Strand = Plus / Plus

                                                                        
Query: 991  gccaaaattagaaacattgaaaatagcaaaaatcatggttctagcaatgttatgaaatgg 1050
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 991  gccaaaattagaaacattgaaaatagcaaaaatcatggttctagcaatgttatgaaatgg 1050

                                                                        
Query: 1051 gcaggtgcaggcacaccaacttgggtcagtagatcttggcgttaaatcattatcattaaa 1110
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1051 gcaggtgcaggcacaccaacttgggtcagtagatcttggcgttaaatcattatcattaaa 1110

                                                                        
Query: 1111 agatatccaagaggaagagagagctcgtgaaaagaaagaacaaccaatcgttgataatgc 1170
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1111 agatatccaagaggaagagagagctcgtgaaaagaaagaacaaccaatcgttgataatgc 1170

                                                          
Query: 1171 atctgactttgaaagaaagaaaccagtatcactctctgatgtcatg 1216
            ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1171 atctgactttgaaagaaagaaaccagtatcactctctgatgtcatg 1216


 Score =  129 bits (65), Expect = 2e-29
 Identities = 65/65 (100%)
 Strand = Plus / Plus

                                                                       
Query: 128 tttacaaggaacattatactcaacaatgtatgttgcatcaacaaaaattaatgcaaattc 187
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 128 tttacaaggaacattatactcaacaatgtatgttgcatcaacaaaaattaatgcaaattc 187

                
Query: 188 aatat 192
           |||||
Sbjct: 188 aatat 192


 Score =  109 bits (55), Expect = 2e-23
 Identities = 64/64 (100%)
 Strand = Plus / Plus

                                                                      
Query: 1  aattatgttgnaacanaaaattgttatgttccancaatacattagttcaattacccaaca 60
          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  aattatgttgnaacanaaaattgttatgttccancaatacattagttcaattacccaaca 60

              
Query: 61 attc 64
          ||||
Sbjct: 61 attc 64


 Score =  103 bits (52), Expect = 1e-21
 Identities = 52/52 (100%)
 Strand = Plus / Plus

                                                                
Query: 2038 tttcaaatattacatcatatatcaatataatctataatatcaaataaataat 2089
            ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2038 tttcaaatattacatcatatatcaatataatctataatatcaaataaataat 2089


 Score = 79.8 bits (40), Expect = 2e-14
 Identities = 40/40 (100%)
 Strand = Plus / Plus

                                                   
Query: 477 tgaagaaatcaacatagaagaggacgaaaatacagttgag 516
           ||||||||||||||||||||||||||||||||||||||||
Sbjct: 477 tgaagaaatcaacatagaagaggacgaaaatacagttgag 516


 Score = 71.9 bits (36), Expect = 4e-12
 Identities = 36/36 (100%)
 Strand = Plus / Plus

                                                
Query: 1309 tttcaacgcctcatcaccatggtcaattgatgaaag 1344
            ||||||||||||||||||||||||||||||||||||
Sbjct: 1309 tttcaacgcctcatcaccatggtcaattgatgaaag 1344


>Contig-U15540-1 (Contig-U15540-1Q) /CSM_Contig/Contig-U15540-1Q.Seq.d
          Length = 3936

 Score = 46.1 bits (23), Expect = 2e-04
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                  
Query: 844 accacaactacaaccaccacaac 866
           |||||||||||||||||||||||
Sbjct: 759 accacaactacaaccaccacaac 781


>Contig-U13933-1 (Contig-U13933-1Q) /CSM_Contig/Contig-U13933-1Q.Seq.d
          Length = 659

 Score = 44.1 bits (22), Expect = 9e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                     
Query: 842 caaccacaactacaaccaccacaaca 867
           |||||||||| |||||||||||||||
Sbjct: 14  caaccacaacaacaaccaccacaaca 39


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 27,503
Number of Sequences: 8402
Number of extensions: 27503
Number of successful extensions: 3794
Number of sequences better than 10.0: 237
length of query: 2130
length of database: 8,075,542
effective HSP length: 17
effective length of query: 2113
effective length of database: 7,932,708
effective search space: 16761812004
effective search space used: 16761812004
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)
dna update 2009. 3.22
Homology vs DNA 
Query= Contig-U16221-1 (Contig-U16221-1Q) /CSM_Contig/Contig-U16221-1Q.Seq.d
         (2130 letters)

Database: ddbj_B
           98,226,423 sequences; 98,766,808,389 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU284716) Dictyostelium discoideum gamete cDNA clone:FC-BI0...   940   0.0    1
(AU270328) Dictyostelium discoideum vegetative cDNA clone:VS...   640   0.0    3
(AU264980) Dictyostelium discoideum vegetative cDNA clone:VS...   759   0.0    1
(AU261321) Dictyostelium discoideum vegetative cDNA clone:VS...   595   e-165  1
(AU265086) Dictyostelium discoideum vegetative cDNA clone:VS...   571   e-158  1
(AU270329) Dictyostelium discoideum vegetative cDNA clone:VS...   208   e-151  4
(BJ437042) Dictyostelium discoideum cDNA clone:ddv33c05, 3' ...   311   e-133  3
(AU265085) Dictyostelium discoideum vegetative cDNA clone:VS...   287   e-126  3
(AU264979) Dictyostelium discoideum vegetative cDNA clone:VS...   287   e-125  3
(BJ444872) Dictyostelium discoideum cDNA clone:ddv57o16, 3' ...   220   3e-85  3
(BJ437961) Dictyostelium discoideum cDNA clone:ddv35m21, 3' ...    80   1e-27  3
(BJ341975) Dictyostelium discoideum cDNA clone:dda9a03, 3' e...    80   4e-10  1
(BJ379914) Dictyostelium discoideum cDNA clone:ddc36m09, 3' ...    74   2e-08  1
(FE852805) CAFP2436.fwd CAFP Pichia stipitis aerobic xylose ...    50   0.048  2
(FE859303) CAFX810.rev CAFX Pichia stipitis oxygen limited x...    50   0.048  2
(FE843996) CAFH1657.rev CAFH Pichia stipitis aerobic dextros...    42   0.069  2
(EY507318) EST_lsal_am_809945 lsalam whole Lepeophtheirus sa...    42   0.072  2
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...    52   0.088  1
(ET346211) S0160K08_T7 CHORI-214 Salmo salar genomic clone S...    44   0.15   3
(AP010610) Lotus japonicus genomic DNA, chromosome 4, clone:...    36   0.21   2
(CO543628) LyEST11252 Sea lamprey LyEST Petromyzon marinus c...    38   0.26   2
(CP000496) Pichia stipitis CBS 6054 chromosome 2, complete s...    50   0.35   1
(AL161516) Arabidopsis thaliana DNA chromosome 4, contig fra...    50   0.35   1
(AL049481) Arabidopsis thaliana DNA chromosome 4, BAC clone ...    50   0.35   1
(EA071984) Sequence 73 from patent US 7179957.                     50   0.35   1
(CX530010) s13dNF56H08MJ073_246056 Methyl Jasmonate-Elicited...    44   0.50   2
(BE248115) NF003G12DT1F1088 Drought Medicago truncatula cDNA...    44   0.50   2
(BG450315) NF031D12DT1F1102 Drought Medicago truncatula cDNA...    44   0.55   2
(BF632575) NF032A01DT1F1002 Drought Medicago truncatula cDNA...    44   0.56   2
(FK714301) pl_061-H10 Ilyanassa (snail) 0-48h Ilyanassa obso...    44   0.60   2
(DH495319) Monosiga ovata DNA, fosmid clone: MOF-059D18, gen...    42   0.70   3
(AC180432) Strongylocentrotus purpuratus clone R3-3103L12, W...    34   0.74   2
(AC184501) Strongylocentrotus purpuratus clone R3-16H10, WOR...    34   0.75   2
(AC181171) Strongylocentrotus purpuratus clone R3-15H9, WORK...    34   0.79   2
(U38197) Dictyostelium discoideum protein tyrosine phosphata...    36   0.91   2
(BJ367792) Dictyostelium discoideum cDNA clone:ddc44c11, 5' ...    36   1.0    2
(CR382138) Debaryomyces hansenii strain CBS767 chromosome F ...    48   1.4    1
(AM482722) Vitis vinifera contig VV78X134816.19, whole genom...    48   1.4    1
(AC117846) Rattus norvegicus clone CH230-247A19, *** SEQUENC...    48   1.4    1
(AC113743) Rattus norvegicus clone CH230-108K21, WORKING DRA...    48   1.4    1
(AC212082) Zea mays chromosome 3 clone CH201-529H1; ZMMBBc05...    48   1.4    1
(BH164504) ENTRH62TF Entamoeba histolytica Sheared DNA Entam...    48   1.4    1
(BH150607) ENTQC43TR Entamoeba histolytica Sheared DNA Entam...    48   1.4    1
(AZ682806) ENTIO28TF Entamoeba histolytica Sheared DNA Entam...    48   1.4    1
(AZ682375) ENTMM38TR Entamoeba histolytica Sheared DNA Entam...    48   1.4    1
(AM667249) Entamoeba terrapinae GSS, clone terra127e01.q1k.        48   1.4    1
(DH489232) Monosiga ovata DNA, fosmid clone: MOF-048G10, gen...    42   1.5    2
(EC824904) SME00007735 esmbsro2 Sawyeria marylandensis cDNA,...    40   2.1    2
(EC825751) SME00002554 esmbsro2 Sawyeria marylandensis cDNA,...    40   2.2    2
(DH470619) Monosiga ovata DNA, fosmid clone: MOF-016B14, gen...    46   2.3    2
(AC111743) Rattus norvegicus clone CH230-155C9, *** SEQUENCI...    34   2.7    2
(AC168681) Strongylocentrotus purpuratus clone R3-3014P16, W...    32   2.7    2
(AC168631) Strongylocentrotus purpuratus clone R3-3018O21, W...    32   2.7    2
(AC179811) Strongylocentrotus purpuratus clone R3-1013K2, WO...    36   2.7    2
(AU269406) Dictyostelium discoideum vegetative cDNA clone:VS...    44   2.9    2
(BJ374552) Dictyostelium discoideum cDNA clone:ddc8m18, 3' e...    44   3.0    2
(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...    38   3.4    6
(FC491188) CAAI11395.fwd CAAI Thalassiosira pseudonana Thaps...    36   3.6    2
(FC480148) CAAC4046.fwd CAAC Thalassiosira pseudonana Total ...    36   3.6    2
(EX858297) CBNF4422.rev CBNF Phycomyces blakesleeanus NRRL15...    36   3.6    2
(EF371053) Pneumocystis jirovecii major surface glycoprotein...    46   5.4    1
(CR382130) Yarrowia lipolytica strain CLIB122 chromosome D c...    46   5.4    1
(BX284746) Neurospora crassa DNA linkage group II BAC contig...    46   5.4    1
(AC144766) Medicago truncatula clone mth2-17c2, complete seq...    46   5.4    1
(AC116960) Dictyostelium discoideum chromosome 2 map complem...    46   5.4    1
(AC131143) Rattus norvegicus clone CH230-76E20, *** SEQUENCI...    46   5.4    1
(AC109728) Rattus norvegicus clone CH230-331B13, *** SEQUENC...    46   5.4    1
(CU695042) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   5.4    1
(AC202335) Medicago truncatula clone mth2-179j17, WORKING DR...    46   5.4    1
(DH503685) Monosiga ovata DNA, fosmid clone: MOF-073K10, gen...    46   5.4    1
(CR502434) mth2-179J17RM1 BAC end, cultivar Jemalong A17 of ...    46   5.4    1
(CE225276) tigr-gss-dog-17000337925587 Dog Library Canis lup...    46   5.4    1
(EL697409) CF_WO0_33f09_SP6 Copepod Whole Organism, Normaliz...    46   5.4    1
(BJ364573) Dictyostelium discoideum cDNA clone:ddc32o04, 5' ...    46   5.4    1
(BB501494) Mus musculus 0 day neonate kidney cDNA, RIKEN ful...    46   5.4    1
(FE264397) CAZO3854.rev CAZO Naegleria gruberi Flagellate St...    46   5.4    1
(FE247222) CAPG9837.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(FE247078) CAPG9762.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(FE246421) CAPG9411.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(FE246272) CAPG9332.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(FE242961) CAPG7416.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(FE241099) CAPG644.rev CAPG Naegleria gruberi amoeba stage N...    46   5.4    1
(FE237873) CAPG4580.rev CAPG Naegleria gruberi amoeba stage ...    46   5.4    1
(EX266237) 1446648_5_I06_023 PY06 Carica papaya cDNA, mRNA s...    46   5.4    1
(ED554976) MUGQ_CH252P132C10Sp6_CN113_045 CHORI-252 Vervet M...    38   6.0    3
(FG126134) LY_YIT_BG0678 German Cockroach Whole Organism cDN...    32   6.8    3
(AC134845) Mus musculus BAC clone RP24-424K5 from chromosome...    44   6.9    2
(BJ365564) Dictyostelium discoideum cDNA clone:ddc35e22, 5' ...    40   7.0    2
(EB626778) AGENCOURT_56961260 D. grimshawi EST Drosophila gr...    32   7.1    3
(EB616995) AGENCOURT_56956408 D. grimshawi EST Drosophila gr...    32   7.2    3
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...    38   7.3    7
(CP000393) Trichodesmium erythraeum IMS101, complete genome.       34   7.3    2
(EB599386) AGENCOURT_56977043 D. grimshawi EST Drosophila gr...    32   7.3    3
(EC384843) PMAH-aab09g10.g1 Lamprey_EST_Olfactory Petromyzon...    44   7.4    2
(EB628381) AGENCOURT_56972392 D. grimshawi EST Drosophila gr...    32   7.6    3
(EB599499) AGENCOURT_56970658 D. grimshawi EST Drosophila gr...    32   7.6    3
(EX846519) CBNC6435.fwd CBNC Phycomyces blakesleeanus NRRL15...    32   7.7    3
(EB616819) AGENCOURT_56970812 D. grimshawi EST Drosophila gr...    32   7.7    3
(BJ384349) Dictyostelium discoideum cDNA clone:ddc49k01, 3' ...    44   7.9    2
(EB622068) AGENCOURT_57015854 D. grimshawi EST Drosophila gr...    32   7.9    3
(BJ383697) Dictyostelium discoideum cDNA clone:ddc53o22, 3' ...    44   8.0    2
(EB602461) AGENCOURT_56932073 D. grimshawi EST Drosophila gr...    32   8.1    3
(BB928926) Trifolium pratense cDNA clone:RCC00242.                 36   8.2    3
(DH496094) Monosiga ovata DNA, fosmid clone: MOF-060J04, gen...    38   8.2    2
(BB940403) Trifolium pratense cDNA clone:RCC14264.                 36   8.3    3
(BB940376) Trifolium pratense cDNA clone:RCC14232.                 36   8.3    3
(BB930894) Trifolium pratense cDNA clone:RCC02627.                 36   8.3    3
(BB929525) Trifolium pratense cDNA clone:RCC00973.                 36   8.3    3
(FE395903) CBTP3120.rev CBTP_Daphnia_pulex_Chosen_One_Librar...    44   8.4    2
(EL503893) A10_560_1.AB1 Blood stage Plasmodium falciparum c...    36   8.5    3
(EX841792) CBNC10978.fwd CBNC Phycomyces blakesleeanus NRRL1...    38   8.5    2
(FE395904) CBTP3120.fwd CBTP_Daphnia_pulex_Chosen_One_Librar...    44   8.5    2
(FD712163) CAAA16991.fwd CAAA Petromyzon marinus Petromyzon ...    44   8.7    2
(DE952261) Macropus eugenii DNA, BAC clone: MEB1-150L06_R, 3...    36   9.2    2
(EB627899) AGENCOURT_57304328 D. grimshawi EST Drosophila gr...    32   9.5    3
(AC130628) Rattus norvegicus clone CH230-186K15, WORKING DRA...    32   9.6    2
(CU311194) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    34   9.7    2
(AC117279) Rattus norvegicus clone CH230-350C21, WORKING DRA...    32   9.7    2
(CR954965) Mouse DNA sequence from clone RP23-19H11 on chrom...    32   9.8    2
(AL670675) Mouse DNA sequence from clone RP23-263N18 on chro...    32   9.8    2
(AL929516) Zebrafish DNA sequence from clone CH211-10C20 in ...    34   9.8    2
(ER449480) 1092963843564 Global-Ocean-Sampling_GS-35-01-01-1...    44   9.9    2
(EB632570) AGENCOURT_57320741 D. grimshawi EST Drosophila gr...    32   9.9    3
(AC181324) Strongylocentrotus purpuratus clone R3-4017O23, W...    32   9.9    2

>(AU284716) Dictyostelium discoideum gamete cDNA clone:FC-BI09, single
            read.
          Length = 586

 Score =  940 bits (474), Expect = 0.0
 Identities = 474/474 (100%)
 Strand = Plus / Plus


Query: 1444 cagtcaataagatgacaagcagaccaactgatgatttctggtcagaacataccaccgcca 1503
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1    cagtcaataagatgacaagcagaccaactgatgatttctggtcagaacataccaccgcca 60


Query: 1504 aaccatcactctctgttagaagtaatgcaccagttcaacaacaagtcggttcattcaacg 1563
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61   aaccatcactctctgttagaagtaatgcaccagttcaacaacaagtcggttcattcaacg 120


Query: 1564 aatatccaaccttaaacattttaccaaatagacaagccaccatcgttagtaagcatacac 1623
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121  aatatccaaccttaaacattttaccaaatagacaagccaccatcgttagtaagcatacac 180


Query: 1624 cagtttcaaccttgaataaaaaaccaacaaccacaaccaccgtaccaggtcaagctagac 1683
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181  cagtttcaaccttgaataaaaaaccaacaaccacaaccaccgtaccaggtcaagctagac 240


Query: 1684 cagatttcatcaaatggtgtcatcaacaattgaaggcattaactagcaatgatgtctctg 1743
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241  cagatttcatcaaatggtgtcatcaacaattgaaggcattaactagcaatgatgtctctg 300


Query: 1744 ttatcactgaactcctttgttcaatgaaaactgaaagtgaaattcgtgaatgcgctcaag 1803
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301  ttatcactgaactcctttgttcaatgaaaactgaaagtgaaattcgtgaatgcgctcaag 360


Query: 1804 aatgtcttggttatagttcagaggtcaatatctttttgaatgaatatcttttagcaagaa 1863
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361  aatgtcttggttatagttcagaggtcaatatctttttgaatgaatatcttttagcaagaa 420


Query: 1864 gtgatgaaccaggtttggcttttgaaagttcaagtccagtcatcacaattccag 1917
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421  gtgatgaaccaggtttggcttttgaaagttcaagtccagtcatcacaattccag 474

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 98226423
Number of Hits to DB: 1,545,023,056
Number of extensions: 97854067
Number of successful extensions: 7538735
Number of sequences better than 10.0: 126
Length of query: 2130
Length of database: 98,766,808,389
Length adjustment: 24
Effective length of query: 2106
Effective length of database: 96,409,374,237
Effective search space: 203038142143122
Effective search space used: 203038142143122
X1: 11 (21.8 bits)
S2: 23 (46.1 bits)
protein update 2009. 7.27
Homology vs Protein 
Query= Contig-U16221-1 (Contig-U16221-1Q) /CSM_Contig/Contig-U16221-1Q.Seq.d
         (2130 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001142_66(CP001142|pid:none) Phaeodactylum tricornutum CCAP 10...    42   0.12
CR382125_1066(CR382125|pid:none) Kluyveromyces lactis strain NRR...    41   0.16
M90654_1(M90654|pid:none) Saccharomyces cerevisiae suppressor of...    40   0.26
(P32909) RecName: Full=Protein SMY2; AltName: Full=Suppressor of...    40   0.26
CR380959_267(CR380959|pid:none) Candida glabrata strain CBS138 c...    39   0.59
CU928169_575(CU928169|pid:none) Kluyveromyces thermotolerans str...    39   0.59
AE016820_578(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...    37   3.8
BC107570_1(BC107570|pid:none) Homo sapiens bromodomain adjacent ...    36   6.5

>CP001142_66(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1055/1
            chromosome 3, complete sequence.
          Length = 997

 Score = 41.6 bits (96), Expect = 0.12
 Identities = 31/127 (24%), Positives = 50/127 (39%)
 Frame = +3

Query: 1497 TAKPSLSVRSNAPVQQQVGSFNEYPTLNILPNRQATIVSKHTPVSTLNKKPXXXXXVPGQ 1676
            T  PS +V++ AP Q Q     +  T     N  A+ VS                    +
Sbjct: 864  TLAPSAAVQARAPPQGQGAMPPKPQTAQQRENMAASSVSDE---------------FGAK 908

Query: 1677 ARPDFIKWCHQQLKALTSNDVSVITELLCSMKTESEIRECAQECLGYSSEVNIFLNEYLL 1856
              P F KWC  Q+  L   D   +     +++   EIR+     LG + +VN F  E++
Sbjct: 909  MSPSFEKWCKDQVYKLNGTDDLTLVAFCMTLQDPGEIRQYLSAYLGSTPQVNSFATEFIN 968

Query: 1857 ARSDEPG 1877
             ++ + G
Sbjct: 969  RKAGKTG 975

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 1,931,375,791
Number of extensions: 28422742
Number of successful extensions: 80992
Number of sequences better than 10.0: 8
Number of HSP's gapped: 80955
Number of HSP's successfully gapped: 8
Length of query: 710
Length of database: 1,061,185,681
Length adjustment: 136
Effective length of query: 574
Effective length of database: 616,676,753
Effective search space: 353972456222
Effective search space used: 353972456222
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.73  gvh: 0.28  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.26  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for Contig-U16221-1 is nuc
VS (DIR, S) 4
VH (FL, L) 3
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 1
FC-IC (SUB) 0