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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U16182-1
Contig ID Contig-U16182-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U16182-1 (Contig-U16182-1Q) /CSM_Contig/Contig-U16182-1Q.Seq.d
CACTGTTGGCCTACTGGAACCTTAATAAAAAAATAAACTCATGTACATAT
AAAAATTAAAATAAAAGAAAAAAAAATGAAAAGGACGATAATAATATTAT
TATTTGTATCATTATTTGTAACATTTGTTTTATCACAAATACCAGATAAT
GATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACAAAC
ACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACT
ATATGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAG
TTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTT
AAATTATACAGCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACAT
TACCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGTGTAAT
GCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGATAA
AAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTA
CTCAATTAAATCAATATATCGAATGGATGCAACAATATAATATTTCAAAT
CCAGTATACCAAACATGGGACGTCTGGAACAATTATGGTCAAGATTTGTT
TGTTCCATCAACAACATGTGATGATTTCACTCAACAATCAATCGAACATT
TAGGTAGTATTGGTATTAATTATAATTGTTCAACAGTTTTCAAGAGAGAT
TACATTAATCTTTTCACTGAAAAACCCCAACCAATCGATTTCACAGAACA
TTATGATGATATCTTTAAATTCTATGAAGCTGTATTAGAAATTAAAGAAG
GCTCAATTGCACAAATCATTGAAAGAATTTTATCAATCATTGGTTTAAAG
AAATATATTTACGTTCAAGGTGAATATTATTTATTAGAATTAAATTATCC
ATTTATGAATGCAAAATATGATTATATCACATTACCAGGTTGTCCAGCAA
ATGGTATCGATTATGAAAATGGCAATTTTATTCATCTCAATTAGATTATT
TTAAAATTAGATTATTTTAAATTAAATTGGTTATAAATAAAAACTAAAAA
CAAAAATAATAAAAAAAAAATATCTTTTTTTTTTTTAATATTTTTCAAAC
TGTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGATAGTTTC
TTTTTTTATTTTCACTTTTTTTTTATTTTGTTTTGAGAAAAAAAAAAAAA
AAAAAAA----------ANTANNGNAGGGGCAATTGNCCNAAGCATTGGA
AGGNNTTTANCNANCNTNGGNTTAAAGGANTNNNTTTACGGNCNAGGNGA
ANATTATTTATTAGAATTAAATTANCCCTTTATGNATGCCAAATATGGTT
ATATCCCCTTACCCGGTTGNCCNGNGAANGGGATCGGTTATGNAA
Gap gap included
Contig length 1435
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 5244659
End point 5245999
Strand (PLUS/MINUS) PLUS
Number of clones 17
Number of EST 25
Link to clone list U16182
List of clone(s) 

est1=VFL220F,1,627
est2=VFC480F,16,596
est3=VFG895E,16,1137
est4=VFJ281F,18,617
est5=VHL857E,18,956
est6=VFH286F,26,531
est7=SSL338F,42,347
est8=SSA660F,51,546
est9=SSB231F,51,496
est10=SFJ661E,74,1119
est11=SSG340F,191,846
est12=SLD812F,223,458
est13=SLE504F,223,881
est14=FC-AD10E,411,1257
est15=SSK458Z,432,1135
est16=SSC271Z,539,1186
est17=FC-AG17Z,555,1162
est18=VFC480Z,604,1135
est19=VFJ281Z,835,1076
est20=VFH286Z,837,1136
est21=SSL338Z,879,1159
est22=SLE504Z,1009,1241
est23=SSG340Z,1012,1226
est24=SLD812Z,1023,1241
est25=VFL220Z,1258,1435
Translated Amino Acid sequence 
tvgllep**knklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqimiqnyvnkefk
erivhkhqyhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiq
qnmmlslk*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkliweq
lmvln*inisngcnniifqiqytkhgtsgtimvkiclfhqqhvmislnnqsni*vvlvli
iivqqfsreitlifslknpnqsisqnimmislnsmkly*klkkaqlhkslkefyqslv*r
niftfkvniiy*n*iihl*mqnmiishyqvvqqmvsimkmailfisirlf*n*iiln*ig
yk*klktkiikkkylfff*yfsncf*KKKKKKKKKKTIVSFFIFTFFLFCFEKKKKKK--
-

---XXXGAIXXSIGRXLXXXGLKXXXYGXGEXYLLELNXPFMXAKYGYIPLPGXPXXGIG
YX
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgtlikk*thvhikikikekkmkrtiiillfvslfvtfvlsqipdndpelcqqriq
redcpqtpvpwgtfndndeievyymqapvfeavfgnffgklggyhsaigfydlttglnyt
aeydaffevgngtlpniinvdgkdellwcnagilctvpyinetywdkniystasktymgt
ingtqlnqyiewmqqynisnpvyqtwdvwnnygqdlfvpsttcddftqqsiehlgsigin
yncstvfkrdyinlftekpqpidftehyddifkfyeavleikegsiaqiierilsiiglk
kyiyvqgeyyllelnypfmnakydyitlpgcpangidyengnfihln*iilkldyfklnw
l*iktknknnkkkisfffliffklflkkkkkkkkkkndsfffyfhfffilf*ekkkkkk-
--

---XXXGAIXXSIGRXLXXXGLKXXXYGXGEXYLLELNXPFMXAKYGYIPLPGXPXXGIG
YX

Frame B:
tvgllep**knklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqimiqnyvnkefk
erivhkhqyhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiq
qnmmlslk*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkliweq
lmvln*inisngcnniifqiqytkhgtsgtimvkiclfhqqhvmislnnqsni*vvlvli
iivqqfsreitlifslknpnqsisqnimmislnsmkly*klkkaqlhkslkefyqslv*r
niftfkvniiy*n*iihl*mqnmiishyqvvqqmvsimkmailfisirlf*n*iiln*ig
yk*klktkiikkkylfff*yfsncf*KKKKKKKKKKTIVSFFIFTFFLFCFEKKKKKK--
-

---xxxgqlxxalegxxxxxx*rxxftxxxxiiy*n*ixplxmpnmvispypvxxxxgsv
mx

Frame C:
llaywnlnkkinsctykn*nkrkknekddnniiiciiicnicfitntr**srimstknsk
rglstntstmgniq*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklys
ri*cfl*srkwyitkyhkcrw*r*tfmv*cwyplycsih**nllg*khlfncfknlygnn
*wysiksiyrmdati*yfkssipnmgrleqlwsrfvcsinnm**fhstinrtfr*ywy*l
*lfnsfqerlh*sfh*ktptnrfhrtl**yl*il*scirn*rrlnctnh*knfinhwfke
iylrsr*ilfiriklsiyecki*lyhitrlsskwyrl*kwqfyssqldyfkirlf*iklv
inkn*kqk**kkniffffnifqtvsekkkkkkkkkkr*flflfslffyfvlrkkkkkk--
-

---xxrgnxpkhwkxfxxxxxkgxxlrxrxxlfiriklxlyxcqiwlypltrlxxexdrl
x
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U16182-1 (Contig-U16182-1Q)
/CSM_Contig/Contig-U16182-1Q.Seq.d
         (1445 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U16182-1 (Contig-U16182-1Q) /CSM_Contig/Conti...  1903  0.0
Contig-U11707-1 (Contig-U11707-1Q) /CSM_Contig/Conti...    46  2e-04
Contig-U04149-1 (Contig-U04149-1Q) /CSM_Contig/Conti...    46  2e-04
Contig-U16045-1 (Contig-U16045-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U15391-1 (Contig-U15391-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U15097-1 (Contig-U15097-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U14805-1 (Contig-U14805-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U05737-1 (Contig-U05737-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U12523-1 (Contig-U12523-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U10129-1 (Contig-U10129-1Q) /CSM_Contig/Conti...    42  0.002

>Contig-U16182-1 (Contig-U16182-1Q) /CSM_Contig/Contig-U16182-1Q.Seq.d
          Length = 1445

 Score = 1903 bits (960), Expect = 0.0
 Identities = 960/960 (100%)
 Strand = Plus / Plus

                                                                        
Query: 77   tgaaaaggacgataataatattattatttgtatcattatttgtaacatttgttttatcac 136
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 77   tgaaaaggacgataataatattattatttgtatcattatttgtaacatttgttttatcac 136

                                                                        
Query: 137  aaataccagataatgatccagaattatgtcaacaaagaattcaaagagaggattgtccac 196
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 137  aaataccagataatgatccagaattatgtcaacaaagaattcaaagagaggattgtccac 196

                                                                        
Query: 197  aaacaccagtaccatggggaacattcaatgacaatgacgaaatcgaagtctactatatgc 256
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 197  aaacaccagtaccatggggaacattcaatgacaatgacgaaatcgaagtctactatatgc 256

                                                                        
Query: 257  aagcaccagttttcgaagcagtttttggaaatttctttggtaagttgggtggatatcatt 316
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 257  aagcaccagttttcgaagcagtttttggaaatttctttggtaagttgggtggatatcatt 316

                                                                        
Query: 317  cagcaattggtttctatgaccttacaactggtttaaattatacagcagaatatgatgctt 376
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 317  cagcaattggtttctatgaccttacaactggtttaaattatacagcagaatatgatgctt 376

                                                                        
Query: 377  tctttgaagtaggaaatggtacattaccaaatatcataaatgtcgatggtaaagatgaac 436
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 377  tctttgaagtaggaaatggtacattaccaaatatcataaatgtcgatggtaaagatgaac 436

                                                                        
Query: 437  ttttatggtgtaatgctggtatcctttgtactgttccatacattaatgaaacttattggg 496
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 437  ttttatggtgtaatgctggtatcctttgtactgttccatacattaatgaaacttattggg 496

                                                                        
Query: 497  ataaaaacatctattcaactgcttcaaaaacttatatgggaacaattaatggtactcaat 556
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 497  ataaaaacatctattcaactgcttcaaaaacttatatgggaacaattaatggtactcaat 556

                                                                        
Query: 557  taaatcaatatatcgaatggatgcaacaatataatatttcaaatccagtataccaaacat 616
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 557  taaatcaatatatcgaatggatgcaacaatataatatttcaaatccagtataccaaacat 616

                                                                        
Query: 617  gggacgtctggaacaattatggtcaagatttgtttgttccatcaacaacatgtgatgatt 676
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 617  gggacgtctggaacaattatggtcaagatttgtttgttccatcaacaacatgtgatgatt 676

                                                                        
Query: 677  tcactcaacaatcaatcgaacatttaggtagtattggtattaattataattgttcaacag 736
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 677  tcactcaacaatcaatcgaacatttaggtagtattggtattaattataattgttcaacag 736

                                                                        
Query: 737  ttttcaagagagattacattaatcttttcactgaaaaaccccaaccaatcgatttcacag 796
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 737  ttttcaagagagattacattaatcttttcactgaaaaaccccaaccaatcgatttcacag 796

                                                                        
Query: 797  aacattatgatgatatctttaaattctatgaagctgtattagaaattaaagaaggctcaa 856
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 797  aacattatgatgatatctttaaattctatgaagctgtattagaaattaaagaaggctcaa 856

                                                                        
Query: 857  ttgcacaaatcattgaaagaattttatcaatcattggtttaaagaaatatatttacgttc 916
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 857  ttgcacaaatcattgaaagaattttatcaatcattggtttaaagaaatatatttacgttc 916

                                                                        
Query: 917  aaggtgaatattatttattagaattaaattatccatttatgaatgcaaaatatgattata 976
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 917  aaggtgaatattatttattagaattaaattatccatttatgaatgcaaaatatgattata 976

                                                                        
Query: 977  tcacattaccaggttgtccagcaaatggtatcgattatgaaaatggcaattttattcatc 1036
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 977  tcacattaccaggttgtccagcaaatggtatcgattatgaaaatggcaattttattcatc 1036


 Score =  188 bits (95), Expect = 2e-47
 Identities = 167/167 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1276 aggggcaattgnccnaagcattggaaggnntttancnancntnggnttaaaggantnnnt 1335
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1276 aggggcaattgnccnaagcattggaaggnntttancnancntnggnttaaaggantnnnt 1335

                                                                        
Query: 1336 ttacggncnaggngaanattatttattagaattaaattanccctttatgnatgccaaata 1395
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1336 ttacggncnaggngaanattatttattagaattaaattanccctttatgnatgccaaata 1395

                                                           
Query: 1396 tggttatatccccttacccggttgnccngngaangggatcggttatg 1442
            |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1396 tggttatatccccttacccggttgnccngngaangggatcggttatg 1442


 Score = 93.7 bits (47), Expect = 7e-19
 Identities = 47/47 (100%)
 Strand = Plus / Plus

                                                           
Query: 1191 cgatagtttctttttttattttcactttttttttattttgttttgag 1237
            |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1191 cgatagtttctttttttattttcactttttttttattttgttttgag 1237


 Score = 77.8 bits (39), Expect = 4e-14
 Identities = 65/75 (86%)
 Strand = Plus / Plus

                                                                        
Query: 1345 aggngaanattatttattagaattaaattanccctttatgnatgccaaatatggttatat 1404
            ||| ||| |||||||||||||||||||||| || |||||| |||| ||||||| ||||||
Sbjct: 918  aggtgaatattatttattagaattaaattatccatttatgaatgcaaaatatgattatat 977

                           
Query: 1405 ccccttacccggttg 1419
            | | ||||| |||||
Sbjct: 978  cacattaccaggttg 992


 Score = 77.8 bits (39), Expect = 4e-14
 Identities = 65/75 (86%)
 Strand = Plus / Plus

                                                                        
Query: 918  aggtgaatattatttattagaattaaattatccatttatgaatgcaaaatatgattatat 977
            ||| ||| |||||||||||||||||||||| || |||||| |||| ||||||| ||||||
Sbjct: 1345 aggngaanattatttattagaattaaattanccctttatgnatgccaaatatggttatat 1404

                           
Query: 978  cacattaccaggttg 992
            | | ||||| |||||
Sbjct: 1405 ccccttacccggttg 1419


 Score = 58.0 bits (29), Expect = 4e-08
 Identities = 43/50 (86%)
 Strand = Plus / Plus

                                                            
Query: 1  cactgttggcctactggaaccttaatnnnnnnntaaactcatgtacatat 50
          ||||||||||||||||||||||||||       |||||||||||||||||
Sbjct: 1  cactgttggcctactggaaccttaataaaaaaataaactcatgtacatat 50


 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                  
Query: 1137 aatatttttcaaactgtttctg 1158
            ||||||||||||||||||||||
Sbjct: 1137 aatatttttcaaactgtttctg 1158


 Score = 32.2 bits (16), Expect = 2.3
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 88  ataataatattattat 103
           ||||||||||||||||
Sbjct: 103 ataataatattattat 88


>Contig-U11707-1 (Contig-U11707-1Q) /CSM_Contig/Contig-U11707-1Q.Seq.d
          Length = 767

 Score = 46.1 bits (23), Expect = 2e-04
 Identities = 31/34 (91%)
 Strand = Plus / Minus

                                              
Query: 1201 tttttttattttcactttttttttattttgtttt 1234
            |||||||||||| | |||||||||||||| ||||
Sbjct: 723  tttttttattttnattttttttttattttttttt 690


>Contig-U04149-1 (Contig-U04149-1Q) /CSM_Contig/Contig-U04149-1Q.Seq.d
          Length = 388

 Score = 46.1 bits (23), Expect = 2e-04
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                   
Query: 1201 tttttttattttcactttttttt 1223
            |||||||||||||||||||||||
Sbjct: 83   tttttttattttcactttttttt 61


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 44,723
Number of Sequences: 8402
Number of extensions: 44723
Number of successful extensions: 8191
Number of sequences better than 10.0: 1655
length of query: 1445
length of database: 8,075,542
effective HSP length: 16
effective length of query: 1429
effective length of database: 7,941,110
effective search space: 11347846190
effective search space used: 11347846190
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 3.20
Homology vs DNA 
Query= Contig-U16182-1 (Contig-U16182-1Q) /CSM_Contig/Contig-U16182-1Q.Seq.d
         (1445 letters)

Database: ddbj_B
           98,226,423 sequences; 98,766,808,389 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061512) Dictyostelium discoideum slug cDNA, clone SLE504.     1296   0.0    1
(C23678) Dictyostelium discoideum gamete cDNA, clone FC-AD10.    1191   0.0    1
(C91571) Dictyostelium discoideum slug cDNA, clone SSK458.       1166   0.0    1
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1035   0.0    6
(BJ415600) Dictyostelium discoideum cDNA clone:ddv23g06, 5' ...  1074   0.0    1
(BJ414588) Dictyostelium discoideum cDNA clone:ddv19a22, 5' ...  1061   0.0    1
(BJ425060) Dictyostelium discoideum cDNA clone:ddv54b16, 5' ...  1059   0.0    1
(BJ413283) Dictyostelium discoideum cDNA clone:ddv14n24, 5' ...  1019   0.0    1
(BJ411824) Dictyostelium discoideum cDNA clone:ddv5p20, 5' e...   839   0.0    3
(BJ402138) Dictyostelium discoideum cDNA clone:dds20i16, 3' ...   890   0.0    2
(BJ389934) Dictyostelium discoideum cDNA clone:dds20i16, 5' ...   769   0.0    3
(AU284270) Dictyostelium discoideum gamete cDNA clone:FC-AG1...   922   0.0    2
(BJ417874) Dictyostelium discoideum cDNA clone:ddv15k22, 5' ...   932   0.0    1
(AU076369) Dictyostelium discoideum slug cDNA, clone SSA660.      920   0.0    1
(C91928) Dictyostelium discoideum slug cDNA, clone SSC271.        884   0.0    2
(BJ431639) Dictyostelium discoideum cDNA clone:ddv14n24, 3' ...   609   0.0    2
(BJ443894) Dictyostelium discoideum cDNA clone:ddv54b16, 3' ...   666   0.0    1
(BJ430065) Dictyostelium discoideum cDNA clone:ddv5p20, 3' e...   593   e-165  1
(AU073191) Dictyostelium discoideum slug cDNA, clone SSG340.      476   e-129  1
(AU061351) Dictyostelium discoideum slug cDNA, clone SLD812.      468   e-127  1
(AU071321) Dictyostelium discoideum slug cDNA, clone SSB231.      412   e-110  1
(AU074596) Dictyostelium discoideum slug cDNA, clone SSL338.      394   e-105  1
(AU038679) Dictyostelium discoideum slug cDNA, clone SSL338.      283   4e-77  2
(BJ431876) Dictyostelium discoideum cDNA clone:ddv15k22, 3' ...   268   5e-67  1
(BJ433838) Dictyostelium discoideum cDNA clone:ddv23g06, 3' ...   188   4e-43  1
(BJ432765) Dictyostelium discoideum cDNA clone:ddv19a22, 3' ...    84   1e-23  2
(AC177746) Strongylocentrotus purpuratus clone R3-1008E12, W...    58   0.001  1
(AU035019) Dictyostelium discoideum slug cDNA, clone SLE504.       44   0.024  2
(AL034557) Plasmodium falciparum MAL4P1.                           52   0.059  1
(EL494627) D03_PFBAMHI-MEDIUM-T7-PLATE2A.AB1 Blood stage Pla...    52   0.059  1
(CJ445701) Macaca fascicularis mRNA, clone: QflA-10854, 5' e...    44   0.086  2
(AC181309) Strongylocentrotus purpuratus clone R3-4017H24, W...    50   0.23   1
(DC241861) Petunia x hybrida cDNA, clone: PET04SEQ05204F.          42   0.29   2
(AE014844) Plasmodium falciparum 3D7 chromosome 12, section ...    42   0.41   9
(CJ453692) Macaca fascicularis mRNA, clone: QflA-19403, 5' e...    36   0.80   3
(CJ456517) Macaca fascicularis mRNA, clone: QflA-22354, 5' e...    34   0.93   3
(AC149952) Strongylocentrotus purpuratus clone R3-32B16, WOR...    48   0.93   1
(AC133307) Rattus norvegicus clone CH230-269I2, WORKING DRAF...    48   0.93   1
(AC126501) Rattus norvegicus clone CH230-206K3, WORKING DRAF...    48   0.93   1
(AC182857) Bos taurus clone CH240-125F22, WORKING DRAFT SEQU...    48   0.93   1
(AC180380) Strongylocentrotus purpuratus clone R3-3113F23, W...    48   0.93   1
(AC176325) Strongylocentrotus purpuratus clone R3-3054K20, W...    48   0.93   1
(BH334420) CH230-110K10.TJ CHORI-230 Segment 1 Rattus norveg...    48   0.93   1
(BH318036) CH230-196J14.TJ CHORI-230 Segment 1 Rattus norveg...    48   0.93   1
(AZ179765) SP_0166_B1_A11_SP6E Strongylocentrotus purpuratus...    48   0.93   1
(CR309794) mte1-31E21RM1 BAC end, cultivar Jemalong A17 of M...    48   0.93   1
(EY356354) CAWZ4313.rev CAWZ Helobdella robusta Primary Late...    48   0.93   1
(EY323665) CAWX9130.rev CAWX Helobdella robusta Primary Earl...    48   0.93   1
(EY312882) CAWX24645.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EY310094) CAWX21957.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EY307254) CAWX20362.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EY301977) CAWX15887.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EY300847) CAWX15248.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EY299555) CAWX14533.rev CAWX Helobdella robusta Primary Ear...    48   0.93   1
(EX472377) NADI-aaa01h09.g1 Rhodnius_prolixus_EST_NADI_5th_i...    48   0.93   1
(AC006278) Plasmodium falciparum chromosome 12 clone PFYAC34...    42   1.1    6
(BJ326558) Dictyostelium discoideum cDNA clone:dda16n21, 5' ...    34   1.2    3
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    36   1.9    14
(EJ575551) 1092960099993 Global-Ocean-Sampling_GS-29-01-01-1...    36   2.4    2
(EJ539688) 1092955355142 Global-Ocean-Sampling_GS-29-01-01-1...    36   2.5    2
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...    38   2.9    8
(AE015934) Staphylococcus epidermidis ATCC 12228 plasmid pSE...    42   3.5    4
(CR450710) Zebrafish DNA sequence from clone CH211-197O6 in ...    46   3.7    1
(CR391906) Zebrafish DNA sequence from clone DKEY-211K10 in ...    46   3.7    1
(BX323873) Zebrafish DNA sequence from clone CH211-133L21 in...    46   3.7    1
(AC170255) Mus musculus chromosome 1, clone RP23-359K18, com...    46   3.7    1
(AC157797) Mus musculus chromosome 1, clone RP24-149G24, com...    46   3.7    1
(AC108909) Mus musculus chromosome 3, clone RP24-245I16, com...    46   3.7    1
(AC102777) Mus musculus chromosome 1, clone RP23-5K7, comple...    46   3.7    1
(CU927919) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   3.7    1
(AP010134) Lotus japonicus genomic DNA, clone: LjT47D03, TM0...    46   3.7    1
(AC188710) Zea mays chromosome 6 clone CH201-11O16; ZMMBBc00...    46   3.7    1
(AC182795) Loxodonta africana clone VMRC15-138E4, WORKING DR...    46   3.7    1
(AC181812) Strongylocentrotus purpuratus clone R3-1024C08, W...    46   3.7    1
(AC176954) Strongylocentrotus purpuratus clone R3-1103J09, W...    46   3.7    1
(AC176551) Strongylocentrotus purpuratus clone R3-3076C11, W...    46   3.7    1
(AC176012) Strongylocentrotus purpuratus clone R3-3028C11, W...    46   3.7    1
(AC168336) Strongylocentrotus purpuratus clone R3-58M23, WOR...    46   3.7    1
(AC168251) Strongylocentrotus purpuratus clone R3-1001J17, W...    46   3.7    1
(EK065459) 1092960117047 Global-Ocean-Sampling_GS-31-01-01-1...    46   3.7    1
(ED849011) OA_CBa0017C20.f OA_CBa Oryza australiensis genomi...    46   3.7    1
(DX392725) GE__Sa0095D11.b1 Gossypium exiguum WGS library Go...    46   3.7    1
(CZ397693) ZMMBF0178M03r ZMMBF Zea mays genomic clone ZMMBF0...    46   3.7    1
(CC346000) OGTAP17TV ZM_0.7_1.5_KB Zea mays genomic clone ZM...    46   3.7    1
(BZ961705) PUFII70TD ZM_0.6_1.0_KB Zea mays genomic clone ZM...    46   3.7    1
(BZ961703) PUFII70TB ZM_0.6_1.0_KB Zea mays genomic clone ZM...    46   3.7    1
(BZ372458) ie67g03.b1 WGS-ZmaysF (DH5a methyl filtered) Zea ...    46   3.7    1
(DT992318) CNB160-F02.x1d-t SHGC-CNB Gasterosteus aculeatus ...    46   3.7    1
(BB908061) Trifolium pratense cDNA clone:RCE06610.                 46   3.7    1
(FE265723) CAZO4668.rev CAZO Naegleria gruberi Flagellate St...    42   4.0    2
(FE261904) CAZO2292.rev CAZO Naegleria gruberi Flagellate St...    42   4.0    2
(FE261905) CAZO2292.fwd CAZO Naegleria gruberi Flagellate St...    42   4.0    2
(FE263484) CAZO3286.fwd CAZO Naegleria gruberi Flagellate St...    42   4.2    2
(CJ490971) Macaca fascicularis mRNA, clone: QtsA-18784, 5' e...    36   4.4    2
(AC202505) Medicago truncatula clone mth2-46p20, WORKING DRA...    44   4.6    7
(AZ686248) ENTLV09TR Entamoeba histolytica Sheared DNA Entam...    38   4.7    2
(AC123538) Sminthopsis macroura clone 79G10, complete sequence.    38   5.0    3
(AE014824) Plasmodium falciparum 3D7 chromosome 14 section 9...    32   5.6    11
(AC176655) Strongylocentrotus purpuratus clone R3-3081B24, W...    40   6.3    2
(AC156825) Bos taurus clone CH240-57I8, WORKING DRAFT SEQUEN...    34   6.6    2
(AC157290) Bos taurus clone CH240-74M18, WORKING DRAFT SEQUE...    34   6.7    2
(AM285301) Spiroplasma citri GII3-3X chromosome, contig Cont...    38   6.8    4
(BJ388722) Dictyostelium discoideum cDNA clone:dds16a04, 5' ...    34   8.0    2
(AE014852) Plasmodium falciparum 3D7 chromosome 12, section ...    30   8.2    12
(EJ123586) 1092343384441 Global-Ocean-Sampling_GS-27-01-01-1...    34   9.1    2
(CJ462214) Macaca fascicularis mRNA, clone: QmoA-15283, 5' e...    34   9.8    3
(CJ442457) Macaca fascicularis mRNA, clone: QccE-19972, 5' e...    34   10.0   3

>(AU061512) Dictyostelium discoideum slug cDNA, clone SLE504.
          Length = 658

 Score = 1296 bits (654), Expect = 0.0
 Identities = 657/658 (99%)
 Strand = Plus / Plus


Query: 223 aatgacaatgacgaaatcgaagtctactatatgcaagcaccagttttcgaagcagttttt 282
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aatgacaatgacgaaatcgaagtctactatatgcaagcaccagttttcgaagcagttttt 60


Query: 283 ggaaatttctttggtaagttgggtggatatcattcagcaattggtttctatgaccttaca 342
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  ggaaatttctttggtaagttgggtggatatcattcagcaattggtttctatgaccttaca 120


Query: 343 actggtttaaattatacagcagaatatgatgctttctttgaagtaggaaatggtacatta 402
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 actggtttaaattatacagcagaatatgatgctttctttgaagtaggaaatggtacatta 180


Query: 403 ccaaatatcataaatgtcgatggtaaagatgaacttttatggtgtaatgctggtatcctt 462
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ccaaatatcataaatgtcgatggtaaagatgaacttttatggtgtaatgctggtatcctt 240


Query: 463 tgtactgttccatacattaatgaaacttattgggataaaaacatctattcaactgcttca 522
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tgtactgttccatacattaatgaaacttattgggataaaaacatctattcaactgcttca 300


Query: 523 aaaacttatatgggaacaattaatggtactcaattaaatcaatatatcgaatggatgcaa 582
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 aaaacttatatgggaacaattaatggtactcaattaaatcaatatatcgaatggatgcaa 360


Query: 583 caatataatatttcaaatccagtataccaaacatgggacgtctggaacaattatggtcaa 642
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 caatataatatttcaaatccagtataccaaacatgggacgtctggaacaattatggtcaa 420


Query: 643 gatttgtttgttccatcaacaacatgtgatgatttcactcaacaatcaatcgaacattta 702
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 gatttgtttgttccatcaacaacatgtgatgatttcactcaacaatcaatcgaacattta 480


Query: 703 ggtagtattggtattaattataattgttcaacagttttcaagagagattacattaatctt 762
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 ggtagtattggtattaattataattgttcaacagttttcaagagagattacattaatctt 540


Query: 763 ttcactgaaaaaccccaaccaatcgatttcacagaacattatgatgatatctttaaattc 822
           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ttcactgaaaaaccacaaccaatcgatttcacagaacattatgatgatatctttaaattc 600


Query: 823 tatgaagctgtattagaaattaaagaaggctcaattgcacaaatcattgaaagaattt 880
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 tatgaagctgtattagaaattaaagaaggctcaattgcacaaatcattgaaagaattt 658

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 98226423
Number of Hits to DB: 1,331,390,409
Number of extensions: 80412365
Number of successful extensions: 6674159
Number of sequences better than 10.0: 109
Length of query: 1445
Length of database: 98,766,808,389
Length adjustment: 24
Effective length of query: 1421
Effective length of database: 96,409,374,237
Effective search space: 136997720790777
Effective search space used: 136997720790777
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.27
Homology vs Protein 
Query= Contig-U16182-1 (Contig-U16182-1Q) /CSM_Contig/Contig-U16182-1Q.Seq.d
         (1445 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   647   0.0
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   340   8e-92
(Q86JG6) RecName: Full=Cln5-like protein 1; Flags: Precursor;  &...   119   2e-25
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...   119   4e-25
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...   117   8e-25
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...   116   2e-24
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...   116   2e-24
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...   115   3e-24
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...   114   7e-24
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...   114   7e-24
(A2TJ54) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...   112   3e-23
CR761663_1(CR761663|pid:none) Xenopus tropicalis finished cDNA, ...   112   3e-23
(Q54WC3) RecName: Full=Cln5-like protein 2; Flags: Precursor;         110   1e-22
AE010299_2044(AE010299|pid:none) Methanosarcina acetivorans str....    39   0.62
NRL(1CPM) Circularly permuted (1-3,1-4)-beta-d-glucan                  37   1.4
NRL(1CPN) Circularly permuted (1-3,1-4)-beta-d-glucan                  36   4.0
BA000040_4983(BA000040|pid:none) Bradyrhizobium japonicum USDA 1...    35   5.3
AE010299_2034(AE010299|pid:none) Methanosarcina acetivorans str....    35   6.9
(Q54UG3) RecName: Full=UPF0746 protein DDB_G0281095;                   35   9.0
CU179680_39(CU179680|pid:none) Mycoplasma agalactiae PG2 chromos...    35   9.0
(Q54UG2) RecName: Full=UPF0746 protein DDB_G0281301;                   35   9.0
(Q54UW0) RecName: Full=UPF0746 protein DDB_G0280785;                   35   9.0

>(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5
            homolog;          Short=Protein CLN5; Flags: Precursor;
          Length = 322

 Score =  647 bits (1670), Expect = 0.0
 Identities = 302/302 (100%), Positives = 302/302 (100%)
 Frame = +1

Query: 136  QIPDNDPELCQQRIQREDCPQTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYH 315
            QIPDNDPELCQQRIQREDCPQTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYH
Sbjct: 21   QIPDNDPELCQQRIQREDCPQTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYH 80

Query: 316  SAIGFYDLTTGLNYTAEYDAFFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYW 495
            SAIGFYDLTTGLNYTAEYDAFFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYW
Sbjct: 81   SAIGFYDLTTGLNYTAEYDAFFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYW 140

Query: 496  DKNIYSTASKTYMGTINGTQLNQYIEWMQQYNISNPVYQTWDVWNNYGQDLFVPSTTCDD 675
            DKNIYSTASKTYMGTINGTQLNQYIEWMQQYNISNPVYQTWDVWNNYGQDLFVPSTTCDD
Sbjct: 141  DKNIYSTASKTYMGTINGTQLNQYIEWMQQYNISNPVYQTWDVWNNYGQDLFVPSTTCDD 200

Query: 676  FTQQSIEHLGSIGINYNCSTVFKRDYINLFTEKPQPIDFTEHYDDIFKFYEAVLEIKEGS 855
            FTQQSIEHLGSIGINYNCSTVFKRDYINLFTEKPQPIDFTEHYDDIFKFYEAVLEIKEGS
Sbjct: 201  FTQQSIEHLGSIGINYNCSTVFKRDYINLFTEKPQPIDFTEHYDDIFKFYEAVLEIKEGS 260

Query: 856  IAQIIERILSIIGLKKYIYVQGEYYLLELNYPFMNAKYDYITLPGCPANGIDYENGNFIH 1035
            IAQIIERILSIIGLKKYIYVQGEYYLLELNYPFMNAKYDYITLPGCPANGIDYENGNFIH
Sbjct: 261  IAQIIERILSIIGLKKYIYVQGEYYLLELNYPFMNAKYDYITLPGCPANGIDYENGNFIH 320

Query: 1036 LN 1041
            LN
Sbjct: 321  LN 322

 Score = 61.2 bits (147), Expect = 9e-08
 Identities = 32/55 (58%), Positives = 33/55 (60%)
 Frame = +2

Query: 1277 GAIXXSIGRXLXXXGLKXXXYGXGEXYLLELNXPFMXAKYGYIPLPGXPXXGIGY 1441
            G+I   I R L   GLK   Y  GE YLLELN PFM AKY YI LPG P  GI Y
Sbjct: 259  GSIAQIIERILSIIGLKKYIYVQGEYYLLELNYPFMNAKYDYITLPGCPANGIDY 313

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 1,962,083,634
Number of extensions: 43480564
Number of successful extensions: 87649
Number of sequences better than 10.0: 22
Number of HSP's gapped: 87543
Number of HSP's successfully gapped: 23
Length of query: 481
Length of database: 1,061,185,681
Length adjustment: 133
Effective length of query: 348
Effective length of database: 626,482,097
Effective search space: 218015769756
Effective search space used: 218015769756
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 1.02  gvh: 0.57  alm: 0.33  top: 0.27  tms: 0.00  mit: 0.30  mip: 0.08
 nuc: 0.52  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: plasma membrane
	  8.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U16182-1 is nuc
VS (DIR, S) 0
VH (FL, L) 1
VF (FL, S) 5
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 2
SS (DIR, S) 6
SH (FL, L) 0
SF (FL, S) 1
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 2
FC-IC (SUB) 0