NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U15464-1
Contig ID Contig-U15464-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U15464-1 (Contig-U15464-1Q) /CSM_Contig/Contig-U15464-1Q.Seq.d
GAAACCGTTATAATAAAATAATAATAATAATAATAATAACATTAATAATA
ATAAAATAAATAAATAAATAAATAAATAAATAATAAAAAAAAAAATAATA
ATAATAATAATAAATAAATTATAATAATAATAATAATAGTAATAATAATA
GTAATAAATAATAANAAAAATATAGTTTTGTTCAACTTAAAAAACCCACT
TAACCACCCACAACAAAACAAAGGGTTAACAATTTTAATTAAAAGTCCAT
CATATTTATTTTCTCAATAATTAAATAAAAAATTGTCAAATTCTAAAAAT
TTTTAGAGATATACATCCAACACCACTACCATCCAAAAACACATAAATAT
ATACACAAATAAATAGCATAAATAAATTTAAACCCACATATTTAAATAAT
GATTAGTATGGGAATGACAAGTAAAGGCCAAAATGTAGATGGGGCACCAG
CTGGTAACAGCTCCTCAGAATGGATTATATCGTCATCTTCTTCACCTTTT
CAAGCAAATAAAAGATATTCATTAGATCCACCATTTGGTTCAGATTATTC
ACCACCAGAATCACCTTTTGATGATATTAGTGAAGCCTTCACAAATTTTT
TAAAACCAATACAGGAAAAATATCATATACCCAAACAAAGAACAACAGTA
TTTACATCAGAATCACAATTACCACCATTGAATACAGATGATGAATTAAC
AAGAAAGTTATTTCAAGATGTTTTTTTACAACATGGTAGAGTAAATCATT
TAACAAGAATTTTAGGTTGGCATCCACAATATTTAGAAAAGTTTCTTCTA
GCATACAATACAATAATGAGAGATCCAGGACCATTACCACTTCATTGGAG
AAATTACATTGGTATACTAGCAGCAGCACGTTACAAATGTAGTTATATTA
TAGCGTTACAAGAACATGAGTTTCTAATGAACAATGGTGATGCACGTTGG
TTACAAGGTATTGATCATATTCCAGCAAAATTAAAAAATTTACTCAAAGT
TATAGAGTTATTGGCTCATCAACCTTGGCTATTGCCAAAATTAGATATAG
AGTATTTGGTAAAAGGTACCGATGCTTGGTCCATCGCTGAATTGGTTCAT
GCCATGGTTTTAATTTGTACATTTCTTTCCTTGTCAGGTTTTGTATTCTG
TTGTGGTATATCACCAGAGTGTGGTCTATCAGAATCAAATAACCTCTCAA
GTAGTTTCTCTCTAAATGATAGTGATTGTGAAATCGAAGATACTGCTGCA
ACCGAAAATACTGCAAAAGTAATGGAATTATTAAAGAATAGAAGATCACA
ACAACAAGATGATGATGATGATGATCAATTACATGATAGACAACAAGATT
TTCATAATGCTGGTGATGATTCTCAATCTTCAAATAACAATACAACAACA
ACTACAACAAAAACCAACTCATCCACAACAAAAACAAACAATTAAATCAA
TTTAAAAAGGTATAATAAAGT
Gap no gap
Contig length 1471
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 2002553
End point 2001143
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 3
Link to clone list U15464
List of clone(s) 

est1=VHH463F,1,527
est2=VHI353F,158,828
est3=VFJ227Z,734,1471
Translated Amino Acid sequence 
nrynkiiiiiiitliiik*ink*ink**kkk******inynnnnnsnnnsnk**xkysfv
qlkkpt*ppttkqrvnnfn*ksiififsiik*kivkf*kfleiyiqhhyhpkthkyihk*
ia*inlnphi*IMISMGMTSKGQNVDGAPAGNSSSEWIISSSSSPFQANKRYSLDPPFGS
DYSPPESPFDDISEAFTNFLKPIQEKYHIPKQRTTVFTSESQLPPLNTDDELTRKLFQDV
FLQHGRVNHLTRILGWHPQYLEKFLLAYNTIMRDPGPLPLHWRNYIGILAAARYKCSYII
ALQEHEFLMNNGDARWLQGIDHIPAKLKNLLKVIELLAHQPWLLPKLDIEYLVKGTDAWS
IAELVHAMVLICTFLSLSGFVFCCGISPECGLSESNNLSSSFSLNDSDCEIEDTAATENT
AKVMELLKNRRSQQQDDDDDDQLHDRQQDFHNAGDDSQSSNNNTTTTTTKTNSSTTKTNN
*inlkrynk
Translated Amino Acid sequence (All Frames) 
Frame A:
etviik*******h****nk*ink*innkkknnnnnnk*iiiiiiiviiivinnxknivl
fnlknplnhpqqnkgltilikspsylfsq*lnkklsnsknf*rytsntttiqkhiniytn
k*hk*i*thifk**lvwe*qvkakm*mghqlvtapqnglyrhllhlfkqikdih*ihhlv
qiihhqnhllmilvkpsqif*nqyrkniiypnkeqqylhqnhnyhh*iqmmn*qesyfkm
ffynmve*ii*qef*vgihni*ksff*htiq**eiqdhyhfigeitlvy*qqhvtnvvil
*ryknmsf**tmvmhvgykvliifqqn*kiyskl*sywlinlgycqn*i*siw*kvpmlg
pslnwfmpwf*fvhffpcqvlysvvvyhqsvvyqnqitsqvvsl*mivivkskillqpki
lqk*wny*riedhnnkmmmmminymidnkifimlvmilnlqitiqqqlqqkpthpqqkqt
iksi*kgiik


Frame B:
kpl**nnnnnnnninnnkink*ink*iikkkiiiiiinkl************iixki*fc
st*kthltthnktkg*qf*lkvhhiyflnn*ikncqilkifrdihptplpsknt*iytqi
nsinkfkptylnnd*ygndk*rpkcrwgtsw*qllrmdyivifftfssk*kifirstiwf
rlfttritf**y**slhkffktntgkisytqtknnsiyiritittieyr**inkkvisrc
ffttw*sksfnknfrlastifrkvsssiqynnersrtittsleklhwytssstlqm*lyy
svtrt*vsneqw*ctlvtry*sysskikkftqsyrvigsstlaiakiryrvfgkryrclv
hr*igschgfnlyisflvrfcillwyitrvwsirik*plk*flsk***l*nrryccnrky
cksngiike*kitttr*****sit**ttrfs*cw**fsifk*qynnnynknqlihnknkq
lnqfkkv**s


Frame C:
nrynkiiiiiiitliiik*ink*ink**kkk******inynnnnnsnnnsnk**xkysfv
qlkkpt*ppttkqrvnnfn*ksiififsiik*kivkf*kfleiyiqhhyhpkthkyihk*
ia*inlnphi*IMISMGMTSKGQNVDGAPAGNSSSEWIISSSSSPFQANKRYSLDPPFGS
DYSPPESPFDDISEAFTNFLKPIQEKYHIPKQRTTVFTSESQLPPLNTDDELTRKLFQDV
FLQHGRVNHLTRILGWHPQYLEKFLLAYNTIMRDPGPLPLHWRNYIGILAAARYKCSYII
ALQEHEFLMNNGDARWLQGIDHIPAKLKNLLKVIELLAHQPWLLPKLDIEYLVKGTDAWS
IAELVHAMVLICTFLSLSGFVFCCGISPECGLSESNNLSSSFSLNDSDCEIEDTAATENT
AKVMELLKNRRSQQQDDDDDDQLHDRQQDFHNAGDDSQSSNNNTTTTTTKTNSSTTKTNN
*inlkrynk
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U15464-1 (Contig-U15464-1Q)
/CSM_Contig/Contig-U15464-1Q.Seq.d
         (1471 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U15464-1 (Contig-U15464-1Q) /CSM_Contig/Conti...  2222  0.0
Contig-U15753-1 (Contig-U15753-1Q) /CSM_Contig/Conti...   628  e-180
Contig-U11363-1 (Contig-U11363-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U11857-1 (Contig-U11857-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U09755-1 (Contig-U09755-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U06678-1 (Contig-U06678-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U05780-1 (Contig-U05780-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U05211-1 (Contig-U05211-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U00011-1 (Contig-U00011-1Q) /CSM_Contig/Conti...    38  0.038
Contig-U14174-1 (Contig-U14174-1Q) /CSM_Contig/Conti...    36  0.15

>Contig-U15464-1 (Contig-U15464-1Q) /CSM_Contig/Contig-U15464-1Q.Seq.d
          Length = 1471

 Score = 2222 bits (1121), Expect = 0.0
 Identities = 1135/1142 (99%)
 Strand = Plus / Plus

                                                                        
Query: 166  aaaaatatagttttgttcaacttaaaaaacccacttaaccacccacaacaaaacaaaggg 225
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 166  aaaaatatagttttgttcaacttaaaaaacccacttaaccacccacaacaaaacaaaggg 225

                                                                        
Query: 226  ttaacaattttaattaaaagtccatcatatttattttctcaataattaaataaaaaattg 285
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 226  ttaacaattttaattaaaagtccatcatatttattttctcaataattaaataaaaaattg 285

                                                                        
Query: 286  tcaaattctaaaaatttttagagatatacatccaacaccactaccatccaaaaacacata 345
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 286  tcaaattctaaaaatttttagagatatacatccaacaccactaccatccaaaaacacata 345

                                                                        
Query: 346  aatatatacacaaataaatagcataaataaatttaaacccacatatttaaataatgatta 405
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 346  aatatatacacaaataaatagcataaataaatttaaacccacatatttaaataatgatta 405

                                                                        
Query: 406  gtatgggaatgacaagtaaaggccaaaatgtagatggggcaccagctggtaacagctcct 465
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 406  gtatgggaatgacaagtaaaggccaaaatgtagatggggcaccagctggtaacagctcct 465

                                                                        
Query: 466  cagaatggattatatcgtcatcttcttcaccttttcaagcaaataaaagatattcattag 525
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 466  cagaatggattatatcgtcatcttcttcaccttttcaagcaaataaaagatattcattag 525

                                                                        
Query: 526  atccaccatttggttcagattattcaccaccagaatcaccttttgatgatattagtgaag 585
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526  atccaccatttggttcagattattcaccaccagaatcaccttttgatgatattagtgaag 585

                                                                        
Query: 586  ccttcacaaattttttaaaaccaatacaggaaaaatatcatatacccaaacaaagaacaa 645
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 586  ccttcacaaattttttaaaaccaatacaggaaaaatatcatatacccaaacaaagaacaa 645

                                                                        
Query: 646  cagtatttacatcagaatcacaattaccaccattgaatacagatgatgaattaacaagaa 705
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 646  cagtatttacatcagaatcacaattaccaccattgaatacagatgatgaattaacaagaa 705

                                                                        
Query: 706  agttatttcaagatgnnnnnnnacaacatggtagagtaaatcatttaacaagaattttag 765
            |||||||||||||||       ||||||||||||||||||||||||||||||||||||||
Sbjct: 706  agttatttcaagatgtttttttacaacatggtagagtaaatcatttaacaagaattttag 765

                                                                        
Query: 766  gttggcatccacaatatttagaaaagtttcttctagcatacaatacaataatgagagatc 825
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 766  gttggcatccacaatatttagaaaagtttcttctagcatacaatacaataatgagagatc 825

                                                                        
Query: 826  caggaccattaccacttcattggagaaattacattggtatactagcagcagcacgttaca 885
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 826  caggaccattaccacttcattggagaaattacattggtatactagcagcagcacgttaca 885

                                                                        
Query: 886  aatgtagttatattatagcgttacaagaacatgagtttctaatgaacaatggtgatgcac 945
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 886  aatgtagttatattatagcgttacaagaacatgagtttctaatgaacaatggtgatgcac 945

                                                                        
Query: 946  gttggttacaaggtattgatcatattccagcaaaattaaaaaatttactcaaagttatag 1005
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 946  gttggttacaaggtattgatcatattccagcaaaattaaaaaatttactcaaagttatag 1005

                                                                        
Query: 1006 agttattggctcatcaaccttggctattgccaaaattagatatagagtatttggtaaaag 1065
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1006 agttattggctcatcaaccttggctattgccaaaattagatatagagtatttggtaaaag 1065

                                                                        
Query: 1066 gtaccgatgcttggtccatcgctgaattggttcatgccatggttttaatttgtacatttc 1125
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1066 gtaccgatgcttggtccatcgctgaattggttcatgccatggttttaatttgtacatttc 1125

                                                                        
Query: 1126 tttccttgtcaggttttgtattctgttgtggtatatcaccagagtgtggtctatcagaat 1185
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1126 tttccttgtcaggttttgtattctgttgtggtatatcaccagagtgtggtctatcagaat 1185

                                                                        
Query: 1186 caaataacctctcaagtagtttctctctaaatgatagtgattgtgaaatcgaagatactg 1245
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1186 caaataacctctcaagtagtttctctctaaatgatagtgattgtgaaatcgaagatactg 1245

                                                                        
Query: 1246 ctgcaaccgaaaatactgcaaaagtaatggaattattaaagaatagaagatcacaacaac 1305
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1246 ctgcaaccgaaaatactgcaaaagtaatggaattattaaagaatagaagatcacaacaac 1305

              
Query: 1306 aa 1307
            ||
Sbjct: 1306 aa 1307


 Score =  129 bits (65), Expect = 1e-29
 Identities = 65/65 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1326 caattacatgatagacaacaagattttcataatgctggtgatgattctcaatcttcaaat 1385
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1326 caattacatgatagacaacaagattttcataatgctggtgatgattctcaatcttcaaat 1385

                 
Query: 1386 aacaa 1390
            |||||
Sbjct: 1386 aacaa 1390


 Score = 60.0 bits (30), Expect = 1e-08
 Identities = 30/30 (100%)
 Strand = Plus / Plus

                                          
Query: 1442 ttaaatcaatttaaaaaggtataataaagt 1471
            ||||||||||||||||||||||||||||||
Sbjct: 1442 ttaaatcaatttaaaaaggtataataaagt 1471


 Score = 32.2 bits (16), Expect = 2.3
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 292 tctaaaaatttttaga 307
           ||||||||||||||||
Sbjct: 307 tctaaaaatttttaga 292


>Contig-U15753-1 (Contig-U15753-1Q) /CSM_Contig/Contig-U15753-1Q.Seq.d
          Length = 823

 Score =  628 bits (317), Expect = e-180
 Identities = 327/329 (99%), Gaps = 1/329 (0%)
 Strand = Plus / Plus

                                                                       
Query: 239 ttaaaagtccatcatatttattttctcaataattaaataaaaaattgtcaaattctaaaa 298
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 82  ttaaaagtccatcatatttattttctcaataattaaataaaaaattgtcaaattctaaaa 141

                                                                       
Query: 299 atttttagagatatacatccaacaccactaccatccaaaaacacataaatatatacacaa 358
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142 atttttagagatatacatccaacaccactaccatccaaaaacacataaatatatacacaa 201

                                                                       
Query: 359 ataaatagcataaataaatttaaacccacatatttaaataatgattagtatgggaatgac 418
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 202 ataaatagcataaataaatttaaacccacatatttaaataatgattagtatgggaatgac 261

                                                                       
Query: 419 aagtaaaggccaaaatgtagatggggcaccagctggtaaca-gctcctcagaatggatta 477
           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 262 aagtaaaggccaaaatgtagatggggcaccagctggtaacatgctcctcagaatggatta 321

                                                                       
Query: 478 tatcgtcatcttcttcaccttttcaagcaaataaaagatattcattagatccaccatttg 537
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 322 tatcgtcatcttcttcaccttttcaagcaaataaaagatattcattagatccaccatttg 381

                                        
Query: 538 gttcagattattcaccaccagaatcacct 566
           ||||||||||||||||||||| |||||||
Sbjct: 382 gttcagattattcaccaccagcatcacct 410


 Score =  283 bits (143), Expect = 4e-76
 Identities = 143/143 (100%)
 Strand = Plus / Plus

                                                                       
Query: 556 cagaatcaccttttgatgatattagtgaagccttcacaaattttttaaaaccaatacagg 615
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 571 cagaatcaccttttgatgatattagtgaagccttcacaaattttttaaaaccaatacagg 630

                                                                       
Query: 616 aaaaatatcatatacccaaacaaagaacaacagtatttacatcagaatcacaattaccac 675
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 631 aaaaatatcatatacccaaacaaagaacaacagtatttacatcagaatcacaattaccac 690

                                  
Query: 676 cattgaatacagatgatgaatta 698
           |||||||||||||||||||||||
Sbjct: 691 cattgaatacagatgatgaatta 713


 Score = 32.2 bits (16), Expect = 2.3
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 292 tctaaaaatttttaga 307
           ||||||||||||||||
Sbjct: 150 tctaaaaatttttaga 135


>Contig-U11363-1 (Contig-U11363-1Q) /CSM_Contig/Contig-U11363-1Q.Seq.d
          Length = 2831

 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                      
Query: 264  tcaataattaaataaaaaattgtcaa 289
            |||||||||||||||||||| |||||
Sbjct: 2791 tcaataattaaataaaaaatcgtcaa 2816


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 30,871
Number of Sequences: 8402
Number of extensions: 30871
Number of successful extensions: 3621
Number of sequences better than 10.0: 406
length of query: 1471
length of database: 8,075,542
effective HSP length: 16
effective length of query: 1455
effective length of database: 7,941,110
effective search space: 11554315050
effective search space used: 11554315050
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 1.27
Homology vs DNA 
Query= Contig-U15464-1 (Contig-U15464-1Q) /CSM_Contig/Contig-U15464-1Q.Seq.d
         (1471 letters)

Database: ddbj_B
           98,226,423 sequences; 98,766,808,389 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432643) Dictyostelium discoideum cDNA clone:ddv19e08, 3' ...  1098   0.0    3
(BJ422961) Dictyostelium discoideum cDNA clone:ddv47j13, 5' ...   634   0.0    3
(BJ422366) Dictyostelium discoideum cDNA clone:ddv45n16, 5' ...   311   e-143  2
(BJ332977) Dictyostelium discoideum cDNA clone:dda41e04, 5' ...   321   e-135  2
(BJ424792) Dictyostelium discoideum cDNA clone:ddv53h18, 5' ...   321   e-132  2
(BJ371366) Dictyostelium discoideum cDNA clone:ddc57p16, 5' ...   321   e-129  2
(BJ414472) Dictyostelium discoideum cDNA clone:ddv19e08, 5' ...   208   6e-77  2
(AU071818) Dictyostelium discoideum slug cDNA, clone SSC575.       78   1e-09  1
(AC216966) Homo sapiens FOSMID clone ABC13-47487500M11 from ...    54   0.004  2
(AC213277) Homo sapiens chromosome 11 clone ABC7_00004182170...    54   0.004  2
(AC226748) Homo sapiens chromosome UNKNOWN clone ABC13-47658...    54   0.005  2
(AC185325) Pan troglodytes BAC clone CH251-302A10 from chrom...    54   0.015  1
(AF391284) Homo sapiens 11p15.5 clone LOH11A, partial sequence.    54   0.015  1
(AC018793) Homo sapiens chromosome 11, clone RP11-437G21, co...    54   0.015  1
(AC016035) Homo sapiens chromosome 11 clone RP11-462E16 map ...    54   0.015  1
(DD103098) Estrogen receptor alpha variants and methods of d...    50   0.24   1
(DD084813) Estrogen Rceptor Alpha Variants and Methods of De...    50   0.24   1
(CS538669) Sequence 46 from Patent EP1772521.                      50   0.24   1
(AX453703) Sequence 1 from Patent WO0234945.                       50   0.24   1
(AX232505) Sequence 3 from Patent WO0162969.                       50   0.24   1
(AX232503) Sequence 1 from Patent WO0162969.                       50   0.24   1
(AY425004) Homo sapiens estrogen receptor 1 (ESR1) gene, com...    50   0.24   1
(AL590993) Human DNA sequence from clone RP11-450E24 on chro...    50   0.24   1
(CP001078) Clostridium botulinum E3 str. Alaska E43, complet...    50   0.24   1
(CP001056) Clostridium botulinum B str. Eklund 17B, complete...    50   0.24   1
(AL844506) Plasmodium falciparum chromosome  7.                    38   0.46   2
(AC173288) Medicago truncatula chromosome 2 BAC clone mth2-1...    48   0.49   4
(AC183290) Pan troglodytes BAC clone CH251-733G21 from chrom...    34   0.52   2
(CR910527) Sus scrofa BES.                                         34   0.67   2
(EK368869) 1095469408084 Global-Ocean-Sampling_GS-31-01-01-1...    30   0.94   4
(CU024887) M.truncatula DNA sequence from clone MTH2-174G4 o...    48   0.94   1
(AC174280) Medicago truncatula clone mth2-11n6, complete seq...    48   0.94   1
(CU914704) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.94   1
(CU856615) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.94   1
(CU468277) M.truncatula DNA sequence *** SEQUENCING CANCELLE...    48   0.94   1
(AC229736) Medicago truncatula clone mth2-7j22, WORKING DRAF...    48   0.94   1
(DT776221) 125673285 TH1 Tribolium castaneum cDNA clone 20C1...    48   0.94   1
(AC117072) Dictyostelium discoideum chromosome 2 map 3879572...    32   0.98   10
(DT800526) 126498695 TL1 Tribolium castaneum cDNA clone 43G7...    34   1.2    3
(AC199002) Zea mays chromosome 3 clone CH201-206E9; ZMMBBc02...    34   1.9    2
(AC191553) Zea mays chromosome 3 clone CH201-328L14; ZMMBBc0...    34   1.9    2
(AC010579) Drosophila melanogaster, chromosome 3R, region 96...    36   1.9    2
(AC010580) Drosophila melanogaster, chromosome 3R, region 96...    36   1.9    2
(AC206510) Zea mays chromosome unknown clone CH201-200A21; Z...    34   1.9    2
(AC204190) Tupaia belangeri clone CH275-49C22, WORKING DRAFT...    34   1.9    2
(AC205217) Tupaia belangeri clone CH275-506C12, WORKING DRAF...    34   1.9    2
(AC017540) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    36   2.0    2
(CG196927) PUIHU60TD ZM_0.6_1.0_KB Zea mays genomic clone ZM...    34   2.5    2
(EV480132) Fc_nor_53B06_M13F Folsomia candida normalized cDN...    34   2.5    2
(BH768516) BMBAC360E03SP6_PSU Brugia malayi Genomic Bac Libr...    38   3.3    2
(ER842195) PPTGB06TF Solanum tuberosum RHPOTKEY BAC ends Sol...    38   3.4    2
(AL390801) Human chromosome 14 DNA sequence BAC R-728G21 of ...    44   3.6    7
(BX908763) Zebrafish DNA sequence from clone DKEY-119O24 in ...    46   3.7    1
(AC138149) Homo sapiens chromosome 11 clone RP11-100F1 map 1...    46   3.7    1
(CU855535) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   3.7    1
(AC068734) Homo sapiens chromosome 12 clone RP11-485M4 map 1...    46   3.7    1
(AC068523) Homo sapiens chromosome 18 clone RP11-620B7 map 1...    46   3.7    1
(AP005230) Homo sapiens genomic DNA, chromosome 18p, clone:R...    46   3.7    1
(EI486077) PV_GBa0100G17.r PV_GBa Phaseolus vulgaris genomic...    46   3.7    1
(EI481958) PV_GBa0094L24.r PV_GBa Phaseolus vulgaris genomic...    46   3.7    1
(EI422548) PV_GBa0011H07.f PV_GBa Phaseolus vulgaris genomic...    46   3.7    1
(CL096663) ISB1-28H15_T7.1 ISB1 Xenopus (Silurana) tropicali...    46   3.7    1
(EL370901) CCES7918.b1_K12.ab1 CCE(LMS) endive Cichorium end...    46   3.7    1
(EL366798) CCES2909.b1_I08.ab1 CCE(LMS) endive Cichorium end...    46   3.7    1
(EL364486) CCEM8999.b1_N17.ab1 CCE(LMS) endive Cichorium end...    46   3.7    1
(EL362629) CCEM7155.b1_E14.ab1 CCE(LMS) endive Cichorium end...    46   3.7    1
(EL358875) CCEM3709.b1_I16.ab1 CCE(LMS) endive Cichorium end...    46   3.7    1
(EL355805) CCEM12391.b1_N01.ab1 CCE(LMS) endive Cichorium en...    46   3.7    1
(EH699536) CCIS11779.b1_E18.ab1 CCI(LMS) chicory Cichorium i...    46   3.7    1
(EH693240) CCIM528.b1_P11.ab1 CCI(LMS) chicory Cichorium int...    46   3.7    1
(DB855483) Nilaparvata lugens mRNA, EST clone:NLNA8698, 5' e...    46   3.7    1
(AE016877) Bacillus cereus ATCC 14579, complete genome.            46   3.7    1
(FH904888) CHO_ONF029xk11f1.ab1 CHO_ONF Nicotiana tabacum ge...    44   4.1    2
(EJ845240) 1093017851671 Global-Ocean-Sampling_GS-30-02-01-1...    36   4.5    2
(AC116330) Dictyostelium discoideum chromosome 2 map 3191214...    36   5.1    8
(AC175963) Strongylocentrotus purpuratus clone R3-17E17, WOR...    34   5.5    6
(AJ925834) Theileria annulata EST, clone tam008h05_p1k.            44   6.0    2
(AC117093) Rattus norvegicus clone CH230-387I8, *** SEQUENCI...    32   6.7    2
(AC129242) Rattus norvegicus clone CH230-278K2, *** SEQUENCI...    32   6.8    2
(AC105738) Sus scrofa clone RP44-258D7, WORKING DRAFT SEQUEN...    32   6.8    2
(AC105437) Sus scrofa clone RP44-49F11, WORKING DRAFT SEQUEN...    32   6.9    2
(AM434007) Vitis vinifera contig VV78X131468.9, whole genome...    36   7.5    4
(AC195565) Medicago truncatula chromosome 2 BAC clone mth2-1...    42   8.2    4
(AM435947) Vitis vinifera contig VV78X265933.2, whole genome...    34   9.2    4
(ED312315) AUAC-aac96d12.b1 Ascaris suum whole genome shotgu...    32   9.3    2
(FK053233) XABT119779.g1 Gateway compatible cien cDNA librar...    32   9.4    2
(EK017763) 1092955250509 Global-Ocean-Sampling_GS-31-01-01-1...    34   9.4    2

>(BJ432643) Dictyostelium discoideum cDNA clone:ddv19e08, 3' end,
            single read.
          Length = 739

 Score = 1098 bits (554), Expect(3) = 0.0
 Identities = 554/554 (100%)
 Strand = Plus / Minus


Query: 754  caagaattttaggttggcatccacaatatttagaaaagtttcttctagcatacaatacaa 813
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 718  caagaattttaggttggcatccacaatatttagaaaagtttcttctagcatacaatacaa 659


Query: 814  taatgagagatccaggaccattaccacttcattggagaaattacattggtatactagcag 873
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 658  taatgagagatccaggaccattaccacttcattggagaaattacattggtatactagcag 599


Query: 874  cagcacgttacaaatgtagttatattatagcgttacaagaacatgagtttctaatgaaca 933
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 598  cagcacgttacaaatgtagttatattatagcgttacaagaacatgagtttctaatgaaca 539


Query: 934  atggtgatgcacgttggttacaaggtattgatcatattccagcaaaattaaaaaatttac 993
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 538  atggtgatgcacgttggttacaaggtattgatcatattccagcaaaattaaaaaatttac 479


Query: 994  tcaaagttatagagttattggctcatcaaccttggctattgccaaaattagatatagagt 1053
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 478  tcaaagttatagagttattggctcatcaaccttggctattgccaaaattagatatagagt 419


Query: 1054 atttggtaaaaggtaccgatgcttggtccatcgctgaattggttcatgccatggttttaa 1113
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 418  atttggtaaaaggtaccgatgcttggtccatcgctgaattggttcatgccatggttttaa 359


Query: 1114 tttgtacatttctttccttgtcaggttttgtattctgttgtggtatatcaccagagtgtg 1173
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 358  tttgtacatttctttccttgtcaggttttgtattctgttgtggtatatcaccagagtgtg 299


Query: 1174 gtctatcagaatcaaataacctctcaagtagtttctctctaaatgatagtgattgtgaaa 1233
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 298  gtctatcagaatcaaataacctctcaagtagtttctctctaaatgatagtgattgtgaaa 239


Query: 1234 tcgaagatactgctgcaaccgaaaatactgcaaaagtaatggaattattaaagaatagaa 1293
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 238  tcgaagatactgctgcaaccgaaaatactgcaaaagtaatggaattattaaagaatagaa 179


Query: 1294 gatcacaacaacaa 1307
            ||||||||||||||
Sbjct: 178  gatcacaacaacaa 165

 Score =  109 bits (55), Expect(3) = 0.0
 Identities = 55/55 (100%)
 Strand = Plus / Minus


Query: 1326 caattacatgatagacaacaagattttcataatgctggtgatgattctcaatctt 1380
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 146  caattacatgatagacaacaagattttcataatgctggtgatgattctcaatctt 92

 Score = 42.1 bits (21), Expect(3) = 0.0
 Identities = 21/21 (100%)
 Strand = Plus / Minus


Query: 734 tggtagagtaaatcatttaac 754
           |||||||||||||||||||||
Sbjct: 739 tggtagagtaaatcatttaac 719

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 98226423
Number of Hits to DB: 1,522,131,189
Number of extensions: 98342500
Number of successful extensions: 8164359
Number of sequences better than 10.0: 87
Length of query: 1471
Length of database: 98,766,808,389
Length adjustment: 24
Effective length of query: 1447
Effective length of database: 96,409,374,237
Effective search space: 139504364520939
Effective search space used: 139504364520939
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.16
Homology vs Protein 
Query= Contig-U15464-1 (Contig-U15464-1Q) /CSM_Contig/Contig-U15464-1Q.Seq.d
         (1471 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WU6) RecName: Full=Sestrin homolog;                               500   e-140
AB168675_1(AB168675|pid:none) Macaca fascicularis testis cDNA cl...   178   4e-43
BT020089_1(BT020089|pid:none) Synthetic construct Homo sapiens p...   178   4e-43
AF033122_1(AF033122|pid:none) Homo sapiens non-p53 regulated PA2...   178   4e-43
CR857066_1(CR857066|pid:none) Pongo abelii mRNA; cDNA DKFZp459D1...   178   5e-43
BC151575_1(BC151575|pid:none) Bos taurus sestrin 1, mRNA (cDNA c...   176   2e-42
BC100403_1(BC100403|pid:none) Mus musculus sestrin 1, mRNA (cDNA...   174   1e-41
(Q9Y6P5) RecName: Full=Sestrin-1; AltName: Full=p53-regulated pr...   169   2e-40
(Q4R6P7) RecName: Full=Sestrin-1;  &AB169134_1(AB169134|pid:none)     169   2e-40
BC149973_1(BC149973|pid:none) Bos taurus sestrin 1, mRNA (cDNA c...   169   2e-40
(P58006) RecName: Full=Sestrin-1; AltName: Full=p53-regulated pr...   168   4e-40
CR760106_1(CR760106|pid:none) Xenopus tropicalis finished cDNA, ...   162   3e-38
BC072823_1(BC072823|pid:none) Xenopus laevis MGC80167 protein, m...   161   5e-38
BC167380_1(BC167380|pid:none) Xenopus tropicalis sestrin 1, mRNA...   158   5e-37
AB168974_1(AB168974|pid:none) Macaca fascicularis testis cDNA, c...   157   7e-37
(Q9CYP7) RecName: Full=Sestrin-3;  &AK017464_1(AK017464|pid:none...   156   2e-36
(Q9W1K5) RecName: Full=Sestrin homolog;  &AE013599_3898(AE013599...   152   2e-35
AK170228_1(AK170228|pid:none) Mus musculus NOD-derived CD11c +ve...   142   2e-32
(P58043) RecName: Full=Sestrin-2;  &AL805897_7(AL805897|pid:none...   142   2e-32
AK170547_1(AK170547|pid:none) Mus musculus NOD-derived CD11c +ve...   142   2e-32
BC045518_1(BC045518|pid:none) Danio rerio sestrin 3, mRNA (cDNA ...   142   4e-32
(P58004) RecName: Full=Sestrin-2; AltName: Full=Hi95;  &AK315710...   141   7e-32
BC166423_1(BC166423|pid:none) Rattus norvegicus similar to Sestr...   140   1e-31
CU104776_4(CU104776|pid:none) Zebrafish DNA sequence from clone ...   140   2e-31
(Q58CN8) RecName: Full=Sestrin-2;  &BC123386_1(BC123386|pid:none)     139   2e-31
BT021909_1(BT021909|pid:none) Bos taurus sestrin 2 (SESN2), mRNA...   139   2e-31
BC068745_1(BC068745|pid:none) Xenopus laevis hypothetical protei...   124   9e-27
DQ439981_1(DQ439981|pid:none) Aedes aegypti clone AE-10233 PA26-...    95   6e-18
AM494955_72(AM494955|pid:none) Leishmania braziliensis chromosom...    43   0.026
AY813903_1(AY813903|pid:none) Schistosoma japonicum SJCHGC09333 ...    38   0.82
AL844504_130(AL844504|pid:none) Plasmodium falciparum 3D7 chromo...    37   1.4
CP001398_127(CP001398|pid:none) Thermococcus gammatolerans EJ3, ...    35   9.1
CP000431_569(CP000431|pid:none) Rhodococcus jostii RHA1, complet...    35   9.1

>(Q54WU6) RecName: Full=Sestrin homolog;
          Length = 601

 Score =  500 bits (1287), Expect = e-140
 Identities = 264/383 (68%), Positives = 269/383 (70%), Gaps = 57/383 (14%)
 Frame = +3

Query: 399  MISMGMTSKGQNVDGAPAGNXXXXXXXXXXXXPFQANKRYSLDPPFGSDYSPPESPFDDI 578
            MISMGMTSKGQNVDGAPAGN            PFQANKRYSLDPPFGSDYSPP SP +++
Sbjct: 1    MISMGMTSKGQNVDGAPAGNSSSEWIISSSSSPFQANKRYSLDPPFGSDYSPPASPQNEL 60

Query: 579  SEAFTNFLKPIQE-------------------------KYHIP----------------- 632
                + FL    E                         K H+P
Sbjct: 61   DPLSSYFLNLQSEDNDVRKTAIEYIINTLSNPDNEKLIKNHLPMIVVLSTESPFDDISEA 120

Query: 633  ---------------KQRTTVFTSESQLPPLNTDDELTRKLFQDVFLQHGRVNHLTRILG 767
                           KQRTTVFTSESQLPPLNTDDELTRKLFQDVFLQHGRVNHLTRILG
Sbjct: 121  FTNFLKPIQEKYHIPKQRTTVFTSESQLPPLNTDDELTRKLFQDVFLQHGRVNHLTRILG 180

Query: 768  WHPQYLEKFLLAYNTIMRDPGPLPLHWRNYIGILAAARYKCSYIIALQEHEFLMNNGDAR 947
            WHPQYLEKFLLAYNTIMRDPGPLPLHWRNYIGILAAARYKCSYIIALQEHEFLMNNGDAR
Sbjct: 181  WHPQYLEKFLLAYNTIMRDPGPLPLHWRNYIGILAAARYKCSYIIALQEHEFLMNNGDAR 240

Query: 948  WLQGIDHIPAKLKNLLKVIELLAHQPWLLPKLDIEYLVKGTDAWSIAELVHAMVLICTFL 1127
            WLQGIDHIPAKLKNLLKVIELLAHQPWLLPKLDIEYLVKGTDAWSIAELVHAMVLICTFL
Sbjct: 241  WLQGIDHIPAKLKNLLKVIELLAHQPWLLPKLDIEYLVKGTDAWSIAELVHAMVLICTFL 300

Query: 1128 SLSGFVFCCGISPECGXXXXXXXXXXXXXXXXDCEIEDTAATENTAKVMELLKNRRSXXX 1307
            SLSGFVFCCGISPECG                DCEIEDTAATENTAKVMELLKNRRS
Sbjct: 301  SLSGFVFCCGISPECGLSESNNLSSSFSLNDSDCEIEDTAATENTAKVMELLKNRRSQQQ 360

Query: 1308 XXXXXXXXXXXXXXFHNAGDDSQ 1376
                          FHNAGDDSQ
Sbjct: 361  DDDDDDQLHDRQQDFHNAGDDSQ 383

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,891,710,159
Number of extensions: 36929565
Number of successful extensions: 92256
Number of sequences better than 10.0: 33
Number of HSP's gapped: 92084
Number of HSP's successfully gapped: 34
Length of query: 490
Length of database: 1,051,180,864
Length adjustment: 133
Effective length of query: 357
Effective length of database: 620,718,517
Effective search space: 221596510569
Effective search space used: 221596510569
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.63  gvh: 0.37  alm: 0.24  top: 0.43  tms: 0.07  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.08  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U15464-1 is cyt
VS (DIR, S) 0
VH (FL, L) 2
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0