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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U15344-1
Contig ID Contig-U15344-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U15344-1 (Contig-U15344-1Q) /CSM_Contig/Contig-U15344-1Q.Seq.d
TCATGCCAGTAAAANACGAANTTTTACCTGTAACTTTCCCTGANTGGGTA
ANGTTTANTGTAANTCCGTTNGCTGCCATTACATTTGGTGTTCAANTAAA
AGTTCATTGGAAANAATTTTGGGTTATNATCATCGNTAGTTACANTGGNG
TAACCGTTGGTAANTGGGGTAGTAAANANTTTGGTNANGTTGGNGCTTTT
TTTGGTTCANCTGCNANTTGTGCTTTAGGTAACCTTTANTCAANATTAAC
AAANANTTTTGCGGTCGTTCCAANTANGGCGGGTANTANTTTACTGGNTC
CTGGTAGTANGGGTGTTAAANGNATCGCTTCAANCANTGANGGTGANANT
TCCNGANGAANTGATTTCATTGGTGGTANGTTTANGACTGCTGCNANTTT
AACNATGGGTTTANTGGTTGCAAANTTAANTANTGCNCCCCCAAAATCGT
TNTAAAAAAAATAATAAAAAAAAAAAAATCTCTAACATTTTAAAATTTAA
AAAAA
Gap no gap
Contig length 505
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 1
Number of EST 1
Link to clone list U15344
List of clone(s) 

est1=CHH435Z,1,505
Translated Amino Acid sequence 
MPVKXEXLPVTFPXWVXFXVXPXAAITFGVQXKVHWKXFWVXIIXSYXGVTVGXWGSKXF
GXVGAFFGSXAXCALGNLXSXLTXXFAVVPXXAGXXLLXPGSXGVKXIASXXXGXXSXXX
DFIGGXFXTAAXLTMGLXVAXLXXAPPKSX*kk**kkknl*hfki*k
Translated Amino Acid sequence (All Frames) 
Frame A:
scq*xtxfyl*lslxg*xlx*xrxlplhlvfx*kfigxnfglxssxvtxx*plvxgvvxx
lvxlxlflvxlxxvl*vtfxqx*qxxlrsfqxxrvxxywxlvvxvlxxslqxxxvxxpxe
xislvvxlxllxx*xwvxwlqx*xxxpqnrxkknnkkkkisnilkfkk


Frame B:
haskxrxftcnfp*xgxvxcxsvxchyiwcsxksslexilgyxhr*lxwxnrw*xg**xx
wxxwxffwfxcxlcfr*plxxinkxfcgrsxxggxxftgsw*xgc*xxrfxx*x*xfxxx
*fhwwxvxdccxfnxgfxgckxxxcxpkivxkkiikkkksltf*nlkk


Frame C:
MPVKXEXLPVTFPXWVXFXVXPXAAITFGVQXKVHWKXFWVXIIXSYXGVTVGXWGSKXF
GXVGAFFGSXAXCALGNLXSXLTXXFAVVPXXAGXXLLXPGSXGVKXIASXXXGXXSXXX
DFIGGXFXTAAXLTMGLXVAXLXXAPPKSX*kk**kkknl*hfki*k
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U15344-1 (Contig-U15344-1Q)
/CSM_Contig/Contig-U15344-1Q.Seq.d
         (505 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U15344-1 (Contig-U15344-1Q) /CSM_Contig/Conti...   543  e-155
Contig-U15762-1 (Contig-U15762-1Q) /CSM_Contig/Conti...   496  e-140
Contig-U15295-1 (Contig-U15295-1Q) /CSM_Contig/Conti...   363  e-100
Contig-U13557-1 (Contig-U13557-1Q) /CSM_Contig/Conti...   357  1e-98
Contig-U15738-1 (Contig-U15738-1Q) /CSM_Contig/Conti...    32  0.78
Contig-U14004-1 (Contig-U14004-1Q) /CSM_Contig/Conti...    32  0.78
Contig-U12480-1 (Contig-U12480-1Q) /CSM_Contig/Conti...    32  0.78
Contig-U15304-1 (Contig-U15304-1Q) /CSM_Contig/Conti...    30  3.1
Contig-U15213-1 (Contig-U15213-1Q) /CSM_Contig/Conti...    30  3.1
Contig-U15083-1 (Contig-U15083-1Q) /CSM_Contig/Conti...    30  3.1

>Contig-U15344-1 (Contig-U15344-1Q) /CSM_Contig/Contig-U15344-1Q.Seq.d
          Length = 505

 Score =  543 bits (274), Expect = e-155
 Identities = 444/451 (98%)
 Strand = Plus / Plus

                                                                       
Query: 1   tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 60

                                                                       
Query: 61  taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 120

                                                                       
Query: 121 gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 180

                                                                       
Query: 181 ttggtnangttggngcnnnnnnnggttcanctgcnanttgtgctttaggtaacctttant 240
           ||||||||||||||||       |||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttggtnangttggngctttttttggttcanctgcnanttgtgctttaggtaacctttant 240

                                                                       
Query: 241 caanattaacaaananttttgcggtcgttccaantanggcgggtantantttactggntc 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 caanattaacaaananttttgcggtcgttccaantanggcgggtantantttactggntc 300

                                                                       
Query: 301 ctggtagtangggtgttaaangnatcgcttcaancantganggtgananttccngangaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 ctggtagtangggtgttaaangnatcgcttcaancantganggtgananttccngangaa 360

                                                                       
Query: 361 ntgatttcattggtggtangtttangactgctgcnantttaacnatgggtttantggttg 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 ntgatttcattggtggtangtttangactgctgcnantttaacnatgggtttantggttg 420

                                          
Query: 421 caaanttaantantgcncccccaaaatcgtt 451
           |||||||||||||||||||||||||||||||
Sbjct: 421 caaanttaantantgcncccccaaaatcgtt 451


 Score = 40.1 bits (20), Expect = 0.003
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 479 tctctaacattttaaaattt 498
           ||||||||||||||||||||
Sbjct: 479 tctctaacattttaaaattt 498


>Contig-U15762-1 (Contig-U15762-1Q) /CSM_Contig/Contig-U15762-1Q.Seq.d
          Length = 2620

 Score =  496 bits (250), Expect = e-140
 Identities = 386/451 (85%)
 Strand = Plus / Plus

                                                                        
Query: 1    tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 60
            |||||||||||| | ||||| |||||||||||||||||||||| ||||||| ||||| ||
Sbjct: 2004 tcatgccagtaagagacgaagttttacctgtaactttccctgattgggtaatgtttattg 2063

                                                                        
Query: 61   taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 120
            ||| |||||| |||||||||||||||||||||||| ||||||||||||||| | ||||||
Sbjct: 2064 taattccgttcgctgccattacatttggtgttcaattaaaagttcattggagagaatttt 2123

                                                                        
Query: 121  gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 180
            ||||||| ||||||| |||||||| ||| |||||||||||||| |||||||||||| | |
Sbjct: 2124 gggttatcatcatcgctagttacattggtgtaaccgttggtaattggggtagtaaatatt 2183

                                                                        
Query: 181  ttggtnangttggngcnnnnnnnggttcanctgcnanttgtgctttaggtaacctttant 240
            ||||| | ||||| ||       |||||| |||| | ||||||| ||||||||||||| |
Sbjct: 2184 ttggtgatgttggtgctttctttggttcagctgcaatttgtgctctaggtaacctttact 2243

                                                                        
Query: 241  caanattaacaaananttttgcggtcgttccaantanggcgggtantantttactggntc 300
            ||| ||||||||| | || |||||||||||||| || |||||||| || |||||||| ||
Sbjct: 2244 caagattaacaaatatttctgcggtcgttccaattatggcgggtattattttactggttc 2303

                                                                        
Query: 301  ctggtagtangggtgttaaangnatcgcttcaancantganggtgananttccngangaa 360
            ||||||||| |||||||||| | |||| ||||| || ||| ||||| | |||| || |||
Sbjct: 2304 ctggtagtatgggtgttaaaggtatcgtttcaatcagtgatggtgatatttccggaggaa 2363

                                                                        
Query: 361  ntgatttcattggtggtangtttangactgctgcnantttaacnatgggtttantggttg 420
             ||||||||||||||||| ||||| ||||||||| | |||||| ||||||||| ||||||
Sbjct: 2364 ttgatttcattggtggtatgtttatgactgctgcaagtttaacaatgggtttattggttg 2423

                                           
Query: 421  caaanttaantantgcncccccaaaatcgtt 451
            |||| |||| || ||| || |||||||||||
Sbjct: 2424 caaatttaattattgctcctccaaaatcgtt 2454


 Score = 40.1 bits (20), Expect = 0.003
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 479  tctctaacattttaaaattt 498
            ||||||||||||||||||||
Sbjct: 2482 tctctaacattttaaaattt 2501


>Contig-U15295-1 (Contig-U15295-1Q) /CSM_Contig/Contig-U15295-1Q.Seq.d
          Length = 553

 Score =  363 bits (183), Expect = e-100
 Identities = 397/448 (88%)
 Strand = Plus / Plus

                                                                       
Query: 1   tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 60
           ||| |||||||||||||||| ||||||||| |||||||||||||||||||||||||| ||
Sbjct: 66  tcangccagtaaaanacgaagttttacctgnaactttccctgantgggtaangtttattg 125

                                                                       
Query: 61  taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 120
           ||||||||||||||||| |||||||||| |||||| |||||||||| |||||||||||||
Sbjct: 126 taantccgttngctgccnttacatttggngttcaattaaaagttcantggaaanaatttt 185

                                                                       
Query: 121 gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 180
           ||| ||| ||||||||||  |||||||||||||||||||| |||||||||| ||||||||
Sbjct: 186 gggntatcatcatcgntanntacantggngtaaccgttggnaantggggtantaaanant 245

                                                                       
Query: 181 ttggtnangttggngcnnnnnnnggttcanctgcnanttgtgctttaggtaacctttant 240
           ||||  ||| ||||||   |   || |||||||| ||||| |||||||| |||| ||| |
Sbjct: 246 ttggngangntggngctttntttggntcanctgcaanttgngctttaggnaaccnttact 305

                                                                       
Query: 241 caanattaacaaananttttgcggtcgttccaantanggcgggtantantttactggntc 300
           ||||||||||||||| ||||||||| ||||||||||||||||| |||| |||||||| ||
Sbjct: 306 caanattaacaaanatttttgcggtngttccaantanggcgggnantattttactggttc 365

                                                                       
Query: 301 ctggtagtangggtgttaaangnatcgcttcaancantganggtgananttccngangaa 360
           ||||||| ||||||||||||||||||| |||||||||||||||||||| ||||||| |||
Sbjct: 366 ctggtagnangggtgttaaangnatcgtttcaancantganggtganatttccngaggaa 425

                                                                       
Query: 361 ntgatttcattggtggtangtttangactgctgcnantttaacnatgggtttantggttg 420
            ||||||| ||||||| ||| ||| ||| || || |||||||||| ||| ||||||| ||
Sbjct: 426 ttgatttcnttggtggnangnttaagacngcngcaantttaacnangggnttantggntg 485

                                       
Query: 421 caaanttaantantgcncccccaaaatc 448
           |||||||||||||||| ||||| |||||
Sbjct: 486 caaanttaantantgcccccccnaaatc 513


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1950
Number of Sequences: 8402
Number of extensions: 1950
Number of successful extensions: 113
Number of sequences better than 10.0: 13
length of query: 505
length of database: 8,075,542
effective HSP length: 16
effective length of query: 489
effective length of database: 7,941,110
effective search space: 3883202790
effective search space used: 3883202790
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 1.24
Homology vs DNA 
Query= Contig-U15344-1 (Contig-U15344-1Q) /CSM_Contig/Contig-U15344-1Q.Seq.d
         (505 letters)

Database: ddbj_B
           98,226,423 sequences; 98,766,808,389 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ379883) Dictyostelium discoideum cDNA clone:ddc36f10, 3' ...   295   e-158  3
(AU034130) Dictyostelium discoideum slug cDNA, clone SLB778.      264   e-144  3
(C25557) Dictyostelium discoideum slug cDNA, clone SLA296.        264   e-134  3
(BJ407614) Dictyostelium discoideum cDNA clone:dds43i06, 3' ...   260   e-127  2
(BJ408987) Dictyostelium discoideum cDNA clone:dds47k16, 3' ...   240   e-124  3
(BJ408333) Dictyostelium discoideum cDNA clone:dds45p11, 3' ...   252   e-124  2
(BJ445250) Dictyostelium discoideum cDNA clone:ddv58m20, 3' ...   260   e-122  2
(BJ407306) Dictyostelium discoideum cDNA clone:dds38k02, 3' ...   260   e-122  2
(AU034523) Dictyostelium discoideum slug cDNA, clone SLC382.      264   e-116  2
(BJ443199) Dictyostelium discoideum cDNA clone:ddv52o03, 3' ...   240   e-101  2
(BJ352408) Dictyostelium discoideum cDNA clone:dda46l16, 3' ...   208   2e-99  2
(BJ437352) Dictyostelium discoideum cDNA clone:ddv34b01, 3' ...   216   2e-96  2
(BJ408785) Dictyostelium discoideum cDNA clone:dds46o23, 3' ...   222   2e-69  3
(AU052901) Dictyostelium discoideum slug cDNA, clone SLF291.      220   5e-59  2
(BJ351474) Dictyostelium discoideum cDNA clone:dda43h15, 3' ...   174   2e-55  3
(BJ404623) Dictyostelium discoideum cDNA clone:dds29i05, 3' ...   168   3e-51  2

>(BJ379883) Dictyostelium discoideum cDNA clone:ddc36f10, 3' end,
           single read.
          Length = 505

 Score =  295 bits (149), Expect(3) = e-158
 Identities = 248/248 (100%)
 Strand = Plus / Minus


Query: 204 ggttcanctgcnanttgtgctttaggtaacctttantcaanattaacaaananttttgcg 263
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 302 ggttcanctgcnanttgtgctttaggtaacctttantcaanattaacaaananttttgcg 243


Query: 264 gtcgttccaantanggcgggtantantttactggntcctggtagtangggtgttaaangn 323
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 242 gtcgttccaantanggcgggtantantttactggntcctggtagtangggtgttaaangn 183


Query: 324 atcgcttcaancantganggtgananttccngangaantgatttcattggtggtangttt 383
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 182 atcgcttcaancantganggtgananttccngangaantgatttcattggtggtangttt 123


Query: 384 angactgctgcnantttaacnatgggtttantggttgcaaanttaantantgcnccccca 443
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122 angactgctgcnantttaacnatgggtttantggttgcaaanttaantantgcnccccca 63


Query: 444 aaatcgtt 451
           ||||||||
Sbjct: 62  aaatcgtt 55

 Score =  276 bits (139), Expect(3) = e-158
 Identities = 193/193 (100%)
 Strand = Plus / Minus


Query: 1   tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 505 tcatgccagtaaaanacgaanttttacctgtaactttccctgantgggtaangtttantg 446


Query: 61  taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 445 taantccgttngctgccattacatttggtgttcaantaaaagttcattggaaanaatttt 386


Query: 121 gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 385 gggttatnatcatcgntagttacantggngtaaccgttggtaantggggtagtaaanant 326


Query: 181 ttggtnangttgg 193
           |||||||||||||
Sbjct: 325 ttggtnangttgg 313

 Score = 40.1 bits (20), Expect(3) = e-158
 Identities = 20/20 (100%)
 Strand = Plus / Minus


Query: 479 tctctaacattttaaaattt 498
           ||||||||||||||||||||
Sbjct: 27  tctctaacattttaaaattt 8

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 98226423
Number of Hits to DB: 160,592,240
Number of extensions: 9792748
Number of successful extensions: 404735
Number of sequences better than 10.0: 16
Length of query: 505
Length of database: 98,766,808,389
Length adjustment: 23
Effective length of query: 482
Effective length of database: 96,507,600,660
Effective search space: 46516663518120
Effective search space used: 46516663518120
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 7.16
Homology vs Protein 
Query= Contig-U15344-1 (Contig-U15344-1Q) /CSM_Contig/Contig-U15344-1Q.Seq.d
         (505 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 386,619,598
Number of extensions: 4764974
Number of successful extensions: 1623
Number of sequences better than 10.0: 0
Number of HSP's gapped: 1623
Number of HSP's successfully gapped: 0
Length of query: 168
Length of database: 1,051,180,864
Length adjustment: 119
Effective length of query: 49
Effective length of database: 666,030,343
Effective search space: 32635486807
Effective search space used: 32635486807
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.91  gvh: 0.54  alm: 0.41  top: 0.63  tms: 0.00  mit: 0.05  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U15344-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0