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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U14993-1
Contig ID Contig-U14993-1
Contig update 2004. 6.11
Contig sequence 
>Contig-U14993-1 (Contig-U14993-1Q) /CSM_Contig/Contig-U14993-1Q.Seq.d
CACTGTTGGCCTACTGGAGTATTCATTTCAATTTTTTTTTTCAAAACACA
CACATAATAAATTTCTTTATTTAAAAACTCATCTTTTTAATTCAATTATC
AACTATTCAATTGAAAAACTCAATTTCAAATACCAAACCTTTCCTTCTAA
ATTTTAATAAATATTCAACAATGGGGGATCGTAATAGCTTCAATAATAGA
GATTATAATAGAGATTATAATAATAATAATAACTATAATAATAATAATAA
TAACTATAATAATAACTATAATAATAATAATAGTAATAATAACAATAGAT
ACGACAATTACAATAACAACAGTTACAATGATAACTACAATAGTGGCCAA
GTTAGATTTGACCATAACAGTCAAAGCAGACACTACCAAAATTATAGAGA
TGATTATTATGGTAATAATAACAACAGAGGATATGACCAAGATAGAAATT
ATTCTACACAACAACAACAAAGATCAAGTTATAATAGCACAAAGGATTAT
GATGACAATAGCAGAGACTATTATTCAGAGAGTAGACAAAATAATCGATC
AGCAAATTATTATGGTAATGAACAAAAAAATTATGTAAATAGTTATAAAT
CTTCATCTTCATCATCATCTTCATCATCTTCATCATATAGACAACAACAA
AGAAATTATAGCAGTGGCNAACACGACNAACTACAACATGAACAACTACA
ACATGAACAACTACAAAATGAACAACAACAACCACAACAAAAAGAAAATA
AAACTCAAGGTATCTTTTCAAAAAATTCATCATTTACTATAGTACCAGAT
GAAGTTGATAAAAATTATAAAGCAGCTGGTGTTTTATTATTTTCAATTGA
TCAAGGTAAAATCAAATTTTTAATGGGATGTGAAGATAGAACTGAAAAGA
GAAAAAAGAATCCAAATTCATCGCTTACCAATGAAGTTTTCTTACCATTT
GGTGGAAAGATTGAAAATGGTGAGATTGCAATAGATACCGCCACCAGAGA
ATTCAATGAAGAGACTGGTTTCATCTTTGAAAATTCACTTCAAGAGTATA
GAGAACTATTTCAATCGGAAAAAACTCAAAAATTTTGGATACCAAATTCA
AAGTATGTTTTATTTTGTGCACCAATACCATTTGATTCAAATATACCTGA
TTTATTTAAATCGGTTGATCGTACCTCTATGAAACATACTGATCAATTGT
TTTTAGAATGGGTTGATCAAAAAGAGGTTTTCAAATGTACTTTTTTTTCT
CCTTTCTTTTTACGTCCTGATGGTTCAAATGGTGAATGTATAAAATTCTT
TTCTTCAATGCTTAGTTTCCTTAAACCATACTTTAGAGAGTTATTAACAC
AATCTCAACAACAACAACAAGAAAATTTAGATTTTACTTTAATTAATAAG
AAAATTAATGAAAATTATATAGAAGATAGTAAAAAACATAATAATAGAAA
TAAAAATAAAAATAAAGATATTACTAGTATTAATACCAATACCAATAATA
ATAACAGTCGTAATAATGAAAATCAAGATAGTTCCAATAGAATTGGTGGT
AGTGGTTTGTTNCTAGATANAAATAANNATAAAAAAATAAAAGGAAAATT
NAACTTTAACAATAAATTAAATACATTAAATAACGAAAAAAAAAATTAAT
AAATATAATAGAAAAATAGAGAAATTAACTGAAGAACTTGAATTGGTTAA
TAATACTAAAGATCTTAAAGATAATAATGATAATAATAACAATAGCAACA
GCAACAGCAATAACCATCATCCAAATAATAGTATTCAAGAATCAATTGTT
ATTGATAAAGTACACACNNAAAGATACGACTCT
Gap no gap
Contig length 1833
Chromosome number (1..6, M) 1
Chromosome length 4919822
Start point 515859
End point 514049
Strand (PLUS/MINUS) MINUS
Number of clones 2
Number of EST 2
Link to clone list U14993
List of clone(s) 

est1=AFI262E,1,1574
est2=VSG383E,1457,1833
Translated Amino Acid sequence 
llaywsihfnfffqnthiinffi*KLIFLIQLSTIQLKNSISNTKPFLLNFNKYSTMGDR
NSFNNRDYNRDYNNNNNYNNNNNNYNNNYNNNNSNNNNRYDNYNNNSYNDNYNSGQVRFD
HNSQSRHYQNYRDDYYGNNNNRGYDQDRNYSTQQQQRSSYNSTKDYDDNSRDYYSESRQN
NRSANYYGNEQKNYVNSYKSSSSSSSSSSSSYRQQQRNYSSGXHDXLQHEQLQHEQLQNE
QQQPQQKENKTQGIFSKNSSFTIVPDEVDKNYKAAGVLLFSIDQGKIKFLMGCEDRTEKR
KKNPNSSLTNEVFLPFGGKIENGEIAIDTATREFNEETGFIFENSLQEYRELFQSEKTQK
FWIPNSKYVLFCAPIPFDSNIPDLFKSVDRTSMKHTDQLFLEWVDQKEVFKCTFFSPFFL
RPDGSNGECIKFFSSMLSFLKPYFRELLTQSQQQQQENLDFTLINKKINENYIEDSKKHN
NRNKNKNKDITSINTNTNNNNSRNNENQDSSNRIGGSGLXLDXNXXKKIKGKXNFNNKLN
TLNNEKKN**i**knrein*rt*ig**y*rs*r******q*qqqqq*pssk**ysrincy
**sthxkirl
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgvfisifffktht**islfknssf*fnyqlfn*ktqfqipnlsf*iliniqqwgi
viasiieiiieiiiiiitiiiiiitiiitiiiiiviitidttitittvtmittivakldl
titvkadttkiiemiimviittedmtkieiilhnnnkdqviiaqrimmtiaetiiqrvdk
iidqqiimvmnkkim*ivinlhlhhhlhhlhhidnnkeiiavanttnynmnnynmnnykm
nnnnhnkkkiklkvsfqkihhll*yqmklikiikqlvfyyfqlikvksnf*wdvkielkr
ekriqihrlpmkfsyhlverlkmvrlq*ipppensmkrlvsslkihfksienyfnrkklk
nfgyqiqsmfyfvhqyhliqiyliylnrlivpl*nilincf*nglikkrfsnvlffllsf
yvlmvqmvnv*nsflqclvslnhtlesy*hnlnnnnkki*ill*lirklmkii*kivkni
iieikikikillvlipipiiitvvimkikivpielvvvvcx*ixixikk*kenxtltin*
ih*itkkkinkynrkieklteelelvnntkdlkdnndnnnnsnsnsnnhhpnnsiqesiv
idkvhtxryds


Frame B:
tvglleysfqfffskhthnkflylkthlfnsiinysieklnfkyqtfpskf**ifnnggs
**lq**rl**rl*****l******l***l********q*irqlq*qqlq**lq*wps*i*
p*qskqtlpkl*r*llw***qqri*pr*klfyttttkikl**hkgl**q*qrllfre*tk
*siskllw**tkklck*l*ififiiifiifii*tttkkl*qwxtrxttt*tttt*tttk*
ttttttkrk*nsrylfkkfiiyystr*s**kl*sswcfiifn*sr*nqifngm*r*n*ke
kkeskfiayq*sfltiwwkd*kw*dcnryrhqriq*rdwfhl*kftsrv*rtisigknsk
ildtkfkvcfilctnti*fkyt*fi*ig*sylyety*sivfrmg*skrgfqmyfffsflf
ts*wfkw*mykilffna*fp*til*rvintisttttrkfrfyfn**en**klyrr**kt*
**k*k*k*ryy*y*yqyq***qs***ksr*fq*nww*wfvxrxk*x*knkrkixl*q*ik
yik*rkkkliniiek*rn*lknlnwliilkilkiimiiitiatataitiiqiivfknqll
likytxkdtt


Frame C:
llaywsihfnfffqnthiinffi*KLIFLIQLSTIQLKNSISNTKPFLLNFNKYSTMGDR
NSFNNRDYNRDYNNNNNYNNNNNNYNNNYNNNNSNNNNRYDNYNNNSYNDNYNSGQVRFD
HNSQSRHYQNYRDDYYGNNNNRGYDQDRNYSTQQQQRSSYNSTKDYDDNSRDYYSESRQN
NRSANYYGNEQKNYVNSYKSSSSSSSSSSSSYRQQQRNYSSGXHDXLQHEQLQHEQLQNE
QQQPQQKENKTQGIFSKNSSFTIVPDEVDKNYKAAGVLLFSIDQGKIKFLMGCEDRTEKR
KKNPNSSLTNEVFLPFGGKIENGEIAIDTATREFNEETGFIFENSLQEYRELFQSEKTQK
FWIPNSKYVLFCAPIPFDSNIPDLFKSVDRTSMKHTDQLFLEWVDQKEVFKCTFFSPFFL
RPDGSNGECIKFFSSMLSFLKPYFRELLTQSQQQQQENLDFTLINKKINENYIEDSKKHN
NRNKNKNKDITSINTNTNNNNSRNNENQDSSNRIGGSGLXLDXNXXKKIKGKXNFNNKLN
TLNNEKKN**i**knrein*rt*ig**y*rs*r******q*qqqqq*pssk**ysrincy
**sthxkirl
own update 2004. 6.23
Homology vs CSM-cDNA 
Query= Contig-U14993-1 (Contig-U14993-1Q)
/CSM_Contig/Contig-U14993-1Q.Seq.d
         (1833 letters)

Database: CSM
           8402 sequences; 8,075,542 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U14993-1 (Contig-U14993-1Q) /CSM_Contig/Conti...  1251  0.0
Contig-U15652-1 (Contig-U15652-1Q) /CSM_Contig/Conti...    54  8e-07
Contig-U10858-1 (Contig-U10858-1Q) /CSM_Contig/Conti...    52  3e-06
Contig-U01359-1 (Contig-U01359-1Q) /CSM_Contig/Conti...    50  1e-05
Contig-U12742-1 (Contig-U12742-1Q) /CSM_Contig/Conti...    48  5e-05
Contig-U15610-1 (Contig-U15610-1Q) /CSM_Contig/Conti...    46  2e-04
Contig-U15821-1 (Contig-U15821-1Q) /CSM_Contig/Conti...    44  8e-04
Contig-U15747-1 (Contig-U15747-1Q) /CSM_Contig/Conti...    44  8e-04
Contig-U12071-1 (Contig-U12071-1Q) /CSM_Contig/Conti...    44  8e-04
Contig-U11749-1 (Contig-U11749-1Q) /CSM_Contig/Conti...    44  8e-04

>Contig-U14993-1 (Contig-U14993-1Q) /CSM_Contig/Contig-U14993-1Q.Seq.d
          Length = 1833

 Score = 1251 bits (631), Expect = 0.0
 Identities = 647/655 (98%)
 Strand = Plus / Plus

                                                                        
Query: 755  tcaaggtatcttttcaaaaaattcatcatttactatagtaccagatgaagttgataaaaa 814
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 755  tcaaggtatcttttcaaaaaattcatcatttactatagtaccagatgaagttgataaaaa 814

                                                                        
Query: 815  ttataaagcagctggtgttttattattttcaattgatcaaggtaaaatcaaatttttaat 874
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 815  ttataaagcagctggtgttttattattttcaattgatcaaggtaaaatcaaatttttaat 874

                                                                        
Query: 875  gggatgtgaagatagaactgaaaagagaaaaaagaatccaaattcatcgcttaccaatga 934
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 875  gggatgtgaagatagaactgaaaagagaaaaaagaatccaaattcatcgcttaccaatga 934

                                                                        
Query: 935  agttttcttaccatttggtggaaagattgaaaatggtgagattgcaatagataccgccac 994
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 935  agttttcttaccatttggtggaaagattgaaaatggtgagattgcaatagataccgccac 994

                                                                        
Query: 995  cagagaattcaatgaagagactggtttcatctttgaaaattcacttcaagagtatagaga 1054
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 995  cagagaattcaatgaagagactggtttcatctttgaaaattcacttcaagagtatagaga 1054

                                                                        
Query: 1055 actatttcaatcggaaaaaactcaaaaattttggataccaaattcaaagtatgttttatt 1114
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1055 actatttcaatcggaaaaaactcaaaaattttggataccaaattcaaagtatgttttatt 1114

                                                                        
Query: 1115 ttgtgcaccaataccatttgattcaaatatacctgatttatttaaatcggttgatcgtac 1174
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1115 ttgtgcaccaataccatttgattcaaatatacctgatttatttaaatcggttgatcgtac 1174

                                                                        
Query: 1175 ctctatgaaacatactgatcaattgtttttagaatgggttgatcaaaaagaggttttcaa 1234
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1175 ctctatgaaacatactgatcaattgtttttagaatgggttgatcaaaaagaggttttcaa 1234

                                                                        
Query: 1235 atgtacnnnnnnnnctcctttctttttacgtcctgatggttcaaatggtgaatgtataaa 1294
            ||||||        ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1235 atgtacttttttttctcctttctttttacgtcctgatggttcaaatggtgaatgtataaa 1294

                                                                        
Query: 1295 attcttttcttcaatgcttagtttccttaaaccatactttagagagttattaacacaatc 1354
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1295 attcttttcttcaatgcttagtttccttaaaccatactttagagagttattaacacaatc 1354

                                                                   
Query: 1355 tcaacaacaacaacaagaaaatttagattttactttaattaataagaaaattaat 1409
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1355 tcaacaacaacaacaagaaaatttagattttactttaattaataagaaaattaat 1409


 Score =  555 bits (280), Expect = e-158
 Identities = 294/301 (97%)
 Strand = Plus / Plus

                                                                       
Query: 298 gatacgacaattacaataacaacagttacaatgataactacaatagtggccaagttagat 357
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 298 gatacgacaattacaataacaacagttacaatgataactacaatagtggccaagttagat 357

                                                                       
Query: 358 ttgaccataacagtcaaagcagacactaccaaaattatagagatgattattatggtaata 417
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 358 ttgaccataacagtcaaagcagacactaccaaaattatagagatgattattatggtaata 417

                                                                       
Query: 418 ataacaacagaggatatgaccaagatagaaattattctacacaacaacaacaaagatcaa 477
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 418 ataacaacagaggatatgaccaagatagaaattattctacacaacaacaacaaagatcaa 477

                                                                       
Query: 478 gttataatagcacaaaggattatgatgacaatagcagagactattattcagagagtagac 537
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 478 gttataatagcacaaaggattatgatgacaatagcagagactattattcagagagtagac 537

                                                                       
Query: 538 aaaataatcgatcagcaaattattatggtaatgaacnnnnnnnttatgtaaatagttata 597
           ||||||||||||||||||||||||||||||||||||       |||||||||||||||||
Sbjct: 538 aaaataatcgatcagcaaattattatggtaatgaacaaaaaaattatgtaaatagttata 597

            
Query: 598 a 598
           |
Sbjct: 598 a 598


 Score =  361 bits (182), Expect = 3e-99
 Identities = 188/188 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1646 ttaataaatataatagaaaaatagagaaattaactgaagaacttgaattggttaataata 1705
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1646 ttaataaatataatagaaaaatagagaaattaactgaagaacttgaattggttaataata 1705

                                                                        
Query: 1706 ctaaagatcttaaagataataatgataataataacaatagcaacagcaacagcaataacc 1765
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1706 ctaaagatcttaaagataataatgataataataacaatagcaacagcaacagcaataacc 1765

                                                                        
Query: 1766 atcatccaaataatagtattcaagaatcaattgttattgataaagtacacacnnaaagat 1825
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1766 atcatccaaataatagtattcaagaatcaattgttattgataaagtacacacnnaaagat 1825

                    
Query: 1826 acgactct 1833
            ||||||||
Sbjct: 1826 acgactct 1833


 Score =  295 bits (149), Expect = 1e-79
 Identities = 169/179 (94%)
 Strand = Plus / Plus

                                                                       
Query: 1   cactgttggcctactggagtattcatttcaannnnnnnnnncaaaacacacacataataa 60
           |||||||||||||||||||||||||||||||          |||||||||||||||||||
Sbjct: 1   cactgttggcctactggagtattcatttcaattttttttttcaaaacacacacataataa 60

                                                                       
Query: 61  atttctttatttaaaaactcatctttttaattcaattatcaactattcaattgaaaaact 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  atttctttatttaaaaactcatctttttaattcaattatcaactattcaattgaaaaact 120

                                                                      
Query: 121 caatttcaaataccaaacctttccttctaaattttaataaatattcaacaatgggggat 179
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 caatttcaaataccaaacctttccttctaaattttaataaatattcaacaatgggggat 179


 Score =  123 bits (62), Expect = 1e-27
 Identities = 65/65 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1504 acagtcgtaataatgaaaatcaagatagttccaatagaattggtggtagtggtttgttnc 1563
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1504 acagtcgtaataatgaaaatcaagatagttccaatagaattggtggtagtggtttgttnc 1563

                 
Query: 1564 tagat 1568
            |||||
Sbjct: 1564 tagat 1568


 Score = 75.8 bits (38), Expect = 2e-13
 Identities = 41/41 (100%)
 Strand = Plus / Plus

                                                    
Query: 637 atagacaacaacaaagaaattatagcagtggcnaacacgac 677
           |||||||||||||||||||||||||||||||||||||||||
Sbjct: 637 atagacaacaacaaagaaattatagcagtggcnaacacgac 677


 Score = 34.2 bits (17), Expect = 0.73
 Identities = 23/25 (92%)
 Strand = Plus / Plus

                                     
Query: 1721 ataataatgataataataacaatag 1745
            ||||||||  |||||||||||||||
Sbjct: 274  ataataatagtaataataacaatag 298


 Score = 32.2 bits (16), Expect = 2.9
 Identities = 19/20 (95%)
 Strand = Plus / Plus

                                
Query: 1721 ataataatgataataataac 1740
            |||||||| |||||||||||
Sbjct: 235  ataataataataataataac 254


>Contig-U15652-1 (Contig-U15652-1Q) /CSM_Contig/Contig-U15652-1Q.Seq.d
          Length = 1036

 Score = 54.0 bits (27), Expect = 8e-07
 Identities = 33/35 (94%)
 Strand = Plus / Plus

                                               
Query: 1720 gataataatgataataataacaatagcaacagcaa 1754
            ||||||||||||||||||||||| | |||||||||
Sbjct: 919  gataataatgataataataacaacaacaacagcaa 953


 Score = 34.2 bits (17), Expect = 0.73
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                             
Query: 1721 ataataatgataataat 1737
            |||||||||||||||||
Sbjct: 911  ataataatgataataat 927


>Contig-U10858-1 (Contig-U10858-1Q) /CSM_Contig/Contig-U10858-1Q.Seq.d
          Length = 2169

 Score = 52.0 bits (26), Expect = 3e-06
 Identities = 32/34 (94%)
 Strand = Plus / Plus

                                              
Query: 1726 aatgataataataacaatagcaacagcaacagca 1759
            ||||||||||||||||||| |||||||| |||||
Sbjct: 382  aatgataataataacaataacaacagcagcagca 415


 Score = 34.2 bits (17), Expect = 0.73
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                             
Query: 1357 aacaacaacaacaagaa 1373
            |||||||||||||||||
Sbjct: 2088 aacaacaacaacaagaa 2104


 Score = 32.2 bits (16), Expect = 2.9
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                          
Query: 2  actgttggcctactgg 17
          ||||||||||||||||
Sbjct: 1  actgttggcctactgg 16


  Database: CSM
    Posted date:  Jun 21, 2004  1:35 PM
  Number of letters in database: 8,075,542
  Number of sequences in database:  8402
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 66,460
Number of Sequences: 8402
Number of extensions: 66460
Number of successful extensions: 21467
Number of sequences better than 10.0: 1585
length of query: 1833
length of database: 8,075,542
effective HSP length: 17
effective length of query: 1816
effective length of database: 7,932,708
effective search space: 14405797728
effective search space used: 14405797728
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)
dna update 2009. 1. 8
Homology vs DNA 
Query= Contig-U14993-1 (Contig-U14993-1Q) /CSM_Contig/Contig-U14993-1Q.Seq.d
         (1833 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ343927) Dictyostelium discoideum cDNA clone:dda17k16, 3' ...   684   0.0    3
(BJ326689) Dictyostelium discoideum cDNA clone:dda17k16, 5' ...   472   0.0    4
(AU266397) Dictyostelium discoideum vegetative cDNA clone:VS...   145   3e-65  3
(AU266398) Dictyostelium discoideum vegetative cDNA clone:VS...   153   3e-44  3
(BJ415438) Dictyostelium discoideum cDNA clone:ddv22g16, 5' ...    36   1e-04  5
(AC116960) Dictyostelium discoideum chromosome 2 map complem...    40   0.003  7
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    40   0.003  10
(BJ367635) Dictyostelium discoideum cDNA clone:ddc43n09, 5' ...    32   0.003  4
(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...    40   0.015  9
(BJ336901) Dictyostelium discoideum cDNA clone:dda55f14, 5' ...    36   0.018  4
(AC116330) Dictyostelium discoideum chromosome 2 map 3191214...    42   0.020  10
(BJ391439) Dictyostelium discoideum cDNA clone:dds13k20, 5' ...    34   0.020  3
(BJ336855) Dictyostelium discoideum cDNA clone:dda55k11, 5' ...    40   0.022  3
(BJ363473) Dictyostelium discoideum cDNA clone:ddc26i19, 5' ...    34   0.024  4
(BJ360545) Dictyostelium discoideum cDNA clone:ddc6l13, 5' e...    34   0.029  4
(BB902774) Trifolium pratense cDNA clone:RCE00465.                 44   0.067  3
(BJ394008) Dictyostelium discoideum cDNA clone:dds33o08, 5' ...    34   0.071  4
(FG073735) UI-FF-IF0-abg-n-15-0-UI.r1 Ceratitis capitata emb...    38   0.073  4
(AU062042) Dictyostelium discoideum slug cDNA, clone SLH324.       50   0.074  2
(BJ327264) Dictyostelium discoideum cDNA clone:dda19f21, 5' ...    36   0.075  3
(BJ388881) Dictyostelium discoideum cDNA clone:dds16k18, 5' ...    36   0.075  3
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...    38   0.076  11
(BK004254) TPA_inf: Homo sapiens olfactory receptor OR11-74 ...    52   0.076  1
(AC194797) Pan troglodytes BAC clone CH251-689E14 from chrom...    52   0.076  1
(EA757300) Sequence 96 from patent US 7374878.                     52   0.076  1
(CS248448) Sequence 375 from Patent EP1612222.                     52   0.076  1
(BD454882) Olfactory and Pheromones G-Protein coupled Recept...    52   0.076  1
(BD443953) HUMAN OLFACTORY RECEPTORS AND GENES ENCODING SAME.      52   0.076  1
(BD144434) Novel G-protein coupled receptors.                      52   0.076  1
(AX646629) Sequence 821 from Patent EP1270724.                     52   0.076  1
(AX551407) Sequence 25 from Patent WO0250276.                      52   0.076  1
(AX448709) Sequence 375 from Patent WO0224726.                     52   0.076  1
(AC111177) Homo sapiens chromosome 11, clone RP11-451K18, co...    52   0.076  1
(AB065820) Homo sapiens gene for seven transmembrane helix r...    52   0.076  1
(AC009642) Homo sapiens chromosome 11 clone 107_B_13 map 11,...    52   0.076  1
(EK323179) 1095462436507 Global-Ocean-Sampling_GS-31-01-01-1...    52   0.076  1
(B58630) CIT-HSP-2013I23.TR CIT-HSP Homo sapiens genomic clo...    52   0.076  1
(AU261973) Dictyostelium discoideum vegetative cDNA clone:VS...    52   0.076  1
(AL034557) Plasmodium falciparum MAL4P1.                           36   0.080  12
(BJ392469) Dictyostelium discoideum cDNA clone:dds27c23, 5' ...    34   0.091  4
(BJ336882) Dictyostelium discoideum cDNA clone:dda55a18, 5' ...    32   0.098  4
(AC115592) Dictyostelium discoideum chromosome 2 map 1-12595...    38   0.11   9
(BJ364231) Dictyostelium discoideum cDNA clone:ddc30m23, 5' ...    34   0.11   3
(EX875269) AUAO6653.rev AUAO Karenia brevis Karbr Dark-phase...    40   0.11   2
(AC026229) Homo sapiens chromosome 5 clone CTD-2276H17, WORK...    48   0.12   6
(AC116967) Dictyostelium discoideum chromosome 2 map 5481549...    40   0.12   4
(EX876963) AUAO7534.rev AUAO Karenia brevis Karbr Dark-phase...    40   0.12   2
(AL606522) Mouse DNA sequence from clone RP23-19L22 on chrom...    44   0.13   7
(EX973352) CAGO6611.rev CAGO Karenia brevis Karbr Stressed (...    40   0.13   2
(ER386461) 1094426009624 Global-Ocean-Sampling_GS-34-01-01-1...    40   0.14   2
(ET524963) fcg3x.227050d16 C. graminicola genomic sequence G...    40   0.15   3
(ED027464) SBA024_L04.r Sugar beet BAC library USH20 (Code S...    38   0.21   3
(C23753) Dictyostelium discoideum gamete cDNA, clone FC-AF03.      44   0.22   3
(BJ325695) Dictyostelium discoideum cDNA clone:dda1d03, 5' e...    34   0.23   3
(CZ176408) MIAA-28M03b.b1 Meloidogyne incognita BAC end sequ...    44   0.25   3
(BJ392512) Dictyostelium discoideum cDNA clone:dds27m22, 5' ...    32   0.25   4
(AF153362) Dictyostelium discoideum adenylyl cyclase (acrA) ...    36   0.26   6
(BJ337151) Dictyostelium discoideum cDNA clone:dda56o09, 5' ...    32   0.26   4
(BJ392471) Dictyostelium discoideum cDNA clone:dds27d20, 5' ...    32   0.27   4
(CZ175702) MIAA-15M14b.g1 Meloidogyne incognita BAC end sequ...    44   0.27   3
(BJ369857) Dictyostelium discoideum cDNA clone:ddc52d08, 5' ...    32   0.28   4
(BJ387526) Dictyostelium discoideum cDNA clone:dds3l06, 5' e...    36   0.28   3
(AC123660) Mus musculus chromosome 3, clone RP23-196E24, com...    42   0.29   5
(ET564726) fcg3x.232450c21 C. graminicola genomic sequence G...    40   0.30   3
(BJ394061) Dictyostelium discoideum cDNA clone:dds33l13, 5' ...    32   0.30   4
(EB282963) CNSN01-F-002452-501 Normalized CNS library (juven...    32   0.30   4
(AC130856) Rattus norvegicus clone CH230-16F14, *** SEQUENCI...    50   0.30   1
(AC130245) Rattus norvegicus clone CH230-302A13, *** SEQUENC...    50   0.30   1
(AC128847) Rattus norvegicus clone CH230-450C15, *** SEQUENC...    50   0.30   1
(BF947481) MR3-NN0220-281000-007-g10 NN0220 Homo sapiens cDN...    50   0.30   1
(BJ396961) Dictyostelium discoideum cDNA clone:dds44g15, 5' ...    32   0.32   4
(CZ174360) MIAA-25L18b.g1 Meloidogyne incognita BAC end sequ...    44   0.33   3
(EC761625) PSE00007865 rw_mgpallid Polysphondylium pallidum ...    32   0.35   4
(CJ464577) Macaca fascicularis mRNA, clone: QnpA-13376, 5' e...    34   0.37   3
(BH465715) BOGMS54TR BOGM Brassica oleracea genomic clone BO...    46   0.46   2
(CU633967) Human DNA sequence *** SEQUENCING IN PROGRESS ***...    44   0.48   3
(CJ452853) Macaca fascicularis mRNA, clone: QflA-18528, 5' e...    40   0.48   2
(BJ364172) Dictyostelium discoideum cDNA clone:ddc30m12, 5' ...    34   0.50   3
(CU469475) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    42   0.50   7
(BJ392279) Dictyostelium discoideum cDNA clone:dds27g03, 5' ...    32   0.53   3
(BJ359413) Dictyostelium discoideum cDNA clone:ddc14l07, 5' ...    34   0.54   3
(AC119513) Rattus norvegicus clone CH230-57B22, *** SEQUENCI...    38   0.63   2
(CT009574) Mouse DNA sequence from clone RP23-140H11 on chro...    34   0.65   9
(AC022108) Homo sapiens chromosome 5 clone CTC-426B10, compl...    48   0.66   6
(BJ396625) Dictyostelium discoideum cDNA clone:dds43i07, 5' ...    36   0.72   3
(BE803076) sr51e07.y1 Gm-c1051 Glycine max cDNA clone GENOME...    42   0.73   3
(CU634019) Human DNA sequence *** SEQUENCING IN PROGRESS ***...    44   0.75   3
(BJ367714) Dictyostelium discoideum cDNA clone:ddc43a23, 5' ...    36   0.78   3
(BJ336918) Dictyostelium discoideum cDNA clone:dda55j16, 5' ...    32   0.79   3
(AL592211) Human DNA sequence from clone RP11-167N5 on chrom...    48   0.80   2
(BJ388457) Dictyostelium discoideum cDNA clone:dds9k18, 5' e...    34   0.81   3
(BJ370112) Dictyostelium discoideum cDNA clone:ddc52n05, 5' ...    32   0.81   4
(BJ362912) Dictyostelium discoideum cDNA clone:ddc24d05, 5' ...    34   0.83   3
(AU269953) Dictyostelium discoideum vegetative cDNA clone:VS...    36   0.84   3
(DQ121066) Hucho taimen clone ZA-6b microsatellite sequence.       32   0.86   3
(BJ386802) Dictyostelium discoideum cDNA clone:dds10c19, 5' ...    32   0.86   3
(BJ396681) Dictyostelium discoideum cDNA clone:dds43f13, 5' ...    32   0.86   4
(BJ389210) Dictyostelium discoideum cDNA clone:dds17n22, 5' ...    36   0.86   3
(BJ392233) Dictyostelium discoideum cDNA clone:dds26l20, 5' ...    30   0.89   4
(BJ392345) Dictyostelium discoideum cDNA clone:dds27f12, 5' ...    36   0.90   3

>(BJ343927) Dictyostelium discoideum cDNA clone:dda17k16, 3' end,
            single read.
          Length = 679

 Score =  684 bits (345), Expect(3) = 0.0
 Identities = 345/345 (100%)
 Strand = Plus / Minus


Query: 896  aaagagaaaaaagaatccaaattcatcgcttaccaatgaagttttcttaccatttggtgg 955
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 679  aaagagaaaaaagaatccaaattcatcgcttaccaatgaagttttcttaccatttggtgg 620


Query: 956  aaagattgaaaatggtgagattgcaatagataccgccaccagagaattcaatgaagagac 1015
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 619  aaagattgaaaatggtgagattgcaatagataccgccaccagagaattcaatgaagagac 560


Query: 1016 tggtttcatctttgaaaattcacttcaagagtatagagaactatttcaatcggaaaaaac 1075
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 559  tggtttcatctttgaaaattcacttcaagagtatagagaactatttcaatcggaaaaaac 500


Query: 1076 tcaaaaattttggataccaaattcaaagtatgttttattttgtgcaccaataccatttga 1135
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 499  tcaaaaattttggataccaaattcaaagtatgttttattttgtgcaccaataccatttga 440


Query: 1136 ttcaaatatacctgatttatttaaatcggttgatcgtacctctatgaaacatactgatca 1195
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 439  ttcaaatatacctgatttatttaaatcggttgatcgtacctctatgaaacatactgatca 380


Query: 1196 attgtttttagaatgggttgatcaaaaagaggttttcaaatgtac 1240
            |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 379  attgtttttagaatgggttgatcaaaaagaggttttcaaatgtac 335

 Score =  278 bits (140), Expect(3) = 0.0
 Identities = 140/140 (100%)
 Strand = Plus / Minus


Query: 1249 ctcctttctttttacgtcctgatggttcaaatggtgaatgtataaaattcttttcttcaa 1308
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 326  ctcctttctttttacgtcctgatggttcaaatggtgaatgtataaaattcttttcttcaa 267


Query: 1309 tgcttagtttccttaaaccatactttagagagttattaacacaatctcaacaacaacaac 1368
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 266  tgcttagtttccttaaaccatactttagagagttattaacacaatctcaacaacaacaac 207


Query: 1369 aagaaaatttagattttact 1388
            ||||||||||||||||||||
Sbjct: 206  aagaaaatttagattttact 187

 Score =  111 bits (56), Expect(3) = 0.0
 Identities = 56/56 (100%)
 Strand = Plus / Minus


Query: 1505 cagtcgtaataatgaaaatcaagatagttccaatagaattggtggtagtggtttgt 1560
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 70   cagtcgtaataatgaaaatcaagatagttccaatagaattggtggtagtggtttgt 15

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,704,239,598
Number of extensions: 117713495
Number of successful extensions: 13246582
Number of sequences better than 10.0: 528
Length of query: 1833
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1809
Effective length of database: 97,308,875,965
Effective search space: 176031756620685
Effective search space used: 176031756620685
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.13
Homology vs Protein 
Query= Contig-U14993-1 (Contig-U14993-1Q) /CSM_Contig/Contig-U14993-1Q.Seq.d
         (1833 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T17825(T17825) hypothetical protein A326L - Chlorella virus PBCV...    45   0.009
DQ491002_457(DQ491002|pid:none) Paramecium bursaria Chlorella vi...    45   0.012
DQ491001_335(DQ491001|pid:none) Paramecium bursaria chlorella vi...    45   0.012
AP008955_3813(AP008955|pid:none) Brevibacillus brevis NBRC 10059...    45   0.012
DQ890022_342(DQ890022|pid:none) Paramecium bursaria Chlorella vi...    43   0.034
AB366653_109(AB366653|pid:none) Ralstonia phage RSL1 DNA, comple...    39   0.49
AP008955_2542(AP008955|pid:none) Brevibacillus brevis NBRC 10059...    39   0.64
CP000089_960(CP000089|pid:none) Dechloromonas aromatica RCB, com...    39   0.64
AM910992_184(AM910992|pid:none) Plasmodium knowlesi strain H chr...    38   1.4
CP000099_2488(CP000099|pid:none) Methanosarcina barkeri str. Fus...    37   1.9
(Q2SSB0) RecName: Full=Chaperone protein dnaK; AltName: Full=Hea...    37   2.4
DQ157866_1(DQ157866|pid:none) Mycoplasma capricolum strain 700 h...    37   2.4
FM954972_409(FM954972|pid:none) Vibrio splendidus LGP32 chromoso...    37   2.4
AF162221_79(AF162221|pid:none) Xestia c-nigrum granulovirus geno...    37   2.4
(Q6MT06) RecName: Full=Chaperone protein dnaK; AltName: Full=Hea...    37   2.4
CP001111_2094(CP001111|pid:none) Stenotrophomonas maltophilia R5...    37   3.2
CP000422_1120(CP000422|pid:none) Pediococcus pentosaceus ATCC 25...    37   3.2
AY229987_61(AY229987|pid:none) Cryptophlebia leucotreta granulov...    36   4.1
AF547984_59(AF547984|pid:none) Adoxophyes orana granulovirus, co...    36   4.1
CP001612_420(CP001612|pid:none) Rickettsia africae ESF-5, comple...    36   5.4
AP009178_92(AP009178|pid:none) Nitratiruptor sp. SB155-2 genomic...    36   5.4
CP000885_1489(CP000885|pid:none) Clostridium phytofermentans ISD...    35   7.0
CP000312_1825(CP000312|pid:none) Clostridium perfringens SM101, ...    35   7.0
AM889285_3150(AM889285|pid:none) Gluconacetobacter diazotrophicu...    35   9.2
CP000246_2103(CP000246|pid:none) Clostridium perfringens ATCC 13...    35   9.2
AM180355_2045(AM180355|pid:none) Clostridium difficile 630 compl...    35   9.2
BA000016_1898(BA000016|pid:none) Clostridium perfringens str. 13...    35   9.2
CP000922_983(CP000922|pid:none) Anoxybacillus flavithermus WK1, ...    35   9.2
CP000886_984(CP000886|pid:none) Salmonella enterica subsp. enter...    35   9.2
BA000028_927(BA000028|pid:none) Oceanobacillus iheyensis HTE831 ...    35   9.2
(Q6F149) RecName: Full=Chaperone protein dnaK; AltName: Full=Hea...    35   9.2

>T17825(T17825) hypothetical protein A326L - Chlorella virus PBCV-1
            &U42580_325(U42580|pid:none)
          Length = 209

 Score = 45.1 bits (105), Expect = 0.009
 Identities = 47/151 (31%), Positives = 69/151 (45%), Gaps = 13/151 (8%)
 Frame = +3

Query: 822  AAGVLLFSI-DQGKIKFLMGCEDRTEKRKKNPNSSLTNEVFLPFGGK---IENGEIAIDT 989
            +AG+L  S  D G++ FL+G + R             + VF  FGGK   ++NG+  I+T
Sbjct: 4    SAGILPISRNDNGEVVFLIGKDSR-------------DNVFSDFGGKTEKVDNGD-PINT 49

Query: 990  ATREFNEETGFIFENSLQEYRELFQSEKTQKFWIPNSK--YVLFCAPIPFDSNIPDLFKS 1163
            ATREF EET     N+  + R   ++          +K  Y +F   +PF  NI   FK
Sbjct: 50   ATREFYEETLGCMCNNPYDVRFRVKNMSIMLMGETKNKNQYRMFVLEVPFVKNINSQFKK 109

Query: 1164 VDRTSMKH-------TDQLFLEWVDQKEVFK 1235
            V    MK+        ++  L WV   E+ K
Sbjct: 110  V-MNFMKYKNIGTNLIEKSELAWVTFNELLK 139

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,669,464,811
Number of extensions: 26703847
Number of successful extensions: 70732
Number of sequences better than 10.0: 31
Number of HSP's gapped: 70595
Number of HSP's successfully gapped: 31
Length of query: 611
Length of database: 1,051,180,864
Length adjustment: 135
Effective length of query: 476
Effective length of database: 614,245,399
Effective search space: 292380809924
Effective search space used: 292380809924
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.91  gvh: 0.46  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U14993-1 is cyt
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0