>Contig-U14647-1 (Contig-U14647-1Q) /CSM_Contig/Contig-U14647-1Q.Seq.d
ACTGTTGGCCTACTGGNAAAACACTTGTATTAAATTGTTAAAAATCTAAA
ATGTTATTCAAATCATTAAATTCAATTGCTAATATCCAAAGTGCATCTCA
AAAGAATCAAATTTCAGGAACTCATTTTTGTTGGAACTTCTCAATCAAAT
AATAATGCTGCTCTTTTAACCCTTGATGCATTAGTTATTATTCATCCAAT
ATTAGCAGTATATGCTCATGTTGACCTTTAAACTGTTTATCTAAAAATAC
CATTCATTTAATAATATAAAACTATTTTAAACTATTTAAATTAAGTTGTT
GTACATTTAAATAAAAATTTATTCCCTTTGGGGAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAA

est1=SHJ818F,1,342
est2=SHJ718Z,17,166
est3=SHJ818Z,18,285
est4=SSC605F,32,415
est5=SSJ136F,32,366
est6=SSL225F,33,387
est7=SSM557E,33,352

tvgllxkhly*ivknlkcysnh*IQLLISKVHLKRIKFQELIFVGTSQSNNNAALLTLDA
LVIIHPILAVYAHVDL*tvylkipfi**yktilnyln*vvvhlnknlfplgkkkkkkkkk
kkkkkkkkkkkkkkkkkk

Frame A:
tvgllxkhly*ivknlkcysnh*IQLLISKVHLKRIKFQELIFVGTSQSNNNAALLTLDA
LVIIHPILAVYAHVDL*tvylkipfi**yktilnyln*vvvhlnknlfplgkkkkkkkkk
kkkkkkkkkkkkkkkkkk


Frame B:
llaywxntcikllki*nviqiikfnc*ypkciskesnfrnsfllellnqiimllf*plmh
*llfiqy*qymlmltfklfi*kyhsfnniklf*ti*ikllyi*ikiyslwgkkkkkkkkk
kkkkkkkkkkkkkkkkk


Frame C:
cwptgktlvlnc*kskmlfkslnsianiqsasqknqisgthfcwnfsik**ccsfnp*ci
syyssnissicsc*plnclskntihlii*nyfklfklscctfk*kfipfgekkkkkkkkk
kkkkkkkkkkkkkkkkk

Query= Contig-U14647-1 (Contig-U14647-1Q)
/CSM_Contig/Contig-U14647-1Q.Seq.d
         (414 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U14647-1 (Contig-U14647-1Q) /CSM_Contig/Conti...   654  0.0
Contig-U04810-1 (Contig-U04810-1Q) /CSM_Contig/Conti...    44  1e-04
Contig-U04738-1 (Contig-U04738-1Q) /CSM_Contig/Conti...    44  1e-04
Contig-U14413-1 (Contig-U14413-1Q) /CSM_Contig/Conti...    42  5e-04
Contig-U11896-1 (Contig-U11896-1Q) /CSM_Contig/Conti...    38  0.007
Contig-U05103-1 (Contig-U05103-1Q) /CSM_Contig/Conti...    38  0.007
Contig-U14809-1 (Contig-U14809-1Q) /CSM_Contig/Conti...    36  0.029
Contig-U14740-1 (Contig-U14740-1Q) /CSM_Contig/Conti...    36  0.029
Contig-U13927-1 (Contig-U13927-1Q) /CSM_Contig/Conti...    36  0.029
Contig-U13706-1 (Contig-U13706-1Q) /CSM_Contig/Conti...    36  0.029

>Contig-U14647-1 (Contig-U14647-1Q) /CSM_Contig/Contig-U14647-1Q.Seq.d
          Length = 414

 Score =  654 bits (330), Expect = 0.0
 Identities = 333/333 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   actgttggcctactggnaaaacacttgtattaaattgttaaaaatctaaaatgttattca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   actgttggcctactggnaaaacacttgtattaaattgttaaaaatctaaaatgttattca 60

                                                                       
Query: 61  aatcattaaattcaattgctaatatccaaagtgcatctcaaaagaatcaaatttcaggaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aatcattaaattcaattgctaatatccaaagtgcatctcaaaagaatcaaatttcaggaa 120

                                                                       
Query: 121 ctcatttttgttggaacttctcaatcaaataataatgctgctcttttaacccttgatgca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 ctcatttttgttggaacttctcaatcaaataataatgctgctcttttaacccttgatgca 180

                                                                       
Query: 181 ttagttattattcatccaatattagcagtatatgctcatgttgacctttaaactgtttat 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttagttattattcatccaatattagcagtatatgctcatgttgacctttaaactgtttat 240

                                                                       
Query: 241 ctaaaaataccattcatttaataatataaaactattttaaactatttaaattaagttgtt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 ctaaaaataccattcatttaataatataaaactattttaaactatttaaattaagttgtt 300

                                            
Query: 301 gtacatttaaataaaaatttattccctttgggg 333
           |||||||||||||||||||||||||||||||||
Sbjct: 301 gtacatttaaataaaaatttattccctttgggg 333


>Contig-U04810-1 (Contig-U04810-1Q) /CSM_Contig/Contig-U04810-1Q.Seq.d
          Length = 478

 Score = 44.1 bits (22), Expect = 1e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 48  aaaatgttattcaaatcattaa 69
           ||||||||||||||||||||||
Sbjct: 112 aaaatgttattcaaatcattaa 133


>Contig-U04738-1 (Contig-U04738-1Q) /CSM_Contig/Contig-U04738-1Q.Seq.d
          Length = 779

 Score = 44.1 bits (22), Expect = 1e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                     
Query: 43  aatctaaaatgttattcaaatcatta 68
           |||| |||||||||||||||||||||
Sbjct: 370 aatcaaaaatgttattcaaatcatta 395


 Score = 30.2 bits (15), Expect = 1.8
 Identities = 18/19 (94%)
 Strand = Plus / Plus

                              
Query: 297 tgttgtacatttaaataaa 315
           ||||||| |||||||||||
Sbjct: 697 tgttgtaaatttaaataaa 715


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 9143
Number of Sequences: 6905
Number of extensions: 9143
Number of successful extensions: 1548
Number of sequences better than 10.0: 777
length of query: 414
length of database: 5,674,871
effective HSP length: 16
effective length of query: 398
effective length of database: 5,564,391
effective search space: 2214627618
effective search space used: 2214627618
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)

Query= Contig-U14647-1 (Contig-U14647-1Q) /CSM_Contig/Contig-U14647-1Q.Seq.d
         (414 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ396538) Dictyostelium discoideum cDNA clone:dds43d06, 5' ...   361   e-158  3
(BJ407588) Dictyostelium discoideum cDNA clone:dds43d06, 3' ...   266   e-127  2
(AU074950) Dictyostelium discoideum slug cDNA, clone SSM557.      278   e-121  2
(AU074534) Dictyostelium discoideum slug cDNA, clone SSL225.      278   e-121  2
(AU073973) Dictyostelium discoideum slug cDNA, clone SSJ136.      246   2e-97  3
(AU071831) Dictyostelium discoideum slug cDNA, clone SSC605.      246   2e-97  3
(C93102) Dictyostelium discoideum slug cDNA, clone SSM557.        323   3e-84  1
(BJ407587) Dictyostelium discoideum cDNA clone:dds43d05, 3' ...   238   1e-58  1
(BJ366346) Dictyostelium discoideum cDNA clone:ddc38l15, 5' ...    50   1e-15  3
(BJ396258) Dictyostelium discoideum cDNA clone:dds42b09, 5' ...    50   1e-15  3
(BJ380585) Dictyostelium discoideum cDNA clone:ddc38l15, 3' ...    50   1e-15  3
(BJ409478) Dictyostelium discoideum cDNA clone:dds42b09, 3' ...    50   1e-15  3
(AU074830) Dictyostelium discoideum slug cDNA, clone SSM194.       50   1e-15  3
(BJ396910) Dictyostelium discoideum cDNA clone:dds44l07, 5' ...    44   7e-14  4
(BJ402595) Dictyostelium discoideum cDNA clone:dds17n12, 3' ...    44   3e-11  4
(BJ389081) Dictyostelium discoideum cDNA clone:dds17n11, 5' ...    44   4e-11  4
(BJ389082) Dictyostelium discoideum cDNA clone:dds17n12, 5' ...    44   4e-11  4
(AU076340) Dictyostelium discoideum slug cDNA, clone SSA615.       48   4e-11  3
(BJ395089) Dictyostelium discoideum cDNA clone:dds37h18, 5' ...    48   4e-11  3
(C92856) Dictyostelium discoideum slug cDNA, clone SSF509.         48   4e-11  3
(C92539) Dictyostelium discoideum slug cDNA, clone SSE588.         48   4e-11  3
(AU037748) Dictyostelium discoideum slug cDNA, clone SSE247.       48   4e-11  3
(BJ407142) Dictyostelium discoideum cDNA clone:dds37h18, 3' ...    48   4e-11  3
(BJ394943) Dictyostelium discoideum cDNA clone:dds37g06, 5' ...    44   2e-10  3
(BJ406983) Dictyostelium discoideum cDNA clone:dds37g06, 3' ...    44   2e-10  3
(BJ390950) Dictyostelium discoideum cDNA clone:dds14j11, 5' ...    44   2e-10  3
(BJ400699) Dictyostelium discoideum cDNA clone:dds14j11, 3' ...    44   2e-10  3
(AU071673) Dictyostelium discoideum slug cDNA, clone SSC243.       44   2e-10  3
(BJ393282) Dictyostelium discoideum cDNA clone:dds30d21, 5' ...    44   6e-10  3
(C90922) Dictyostelium discoideum slug cDNA, clone SSJ313.         44   6e-10  3
(C84222) Dictyostelium discoideum slug cDNA, clone SSB337.         44   6e-10  3
(C92972) Dictyostelium discoideum slug cDNA, clone SSF295.         44   6e-10  3
(C89764) Dictyostelium discoideum slug cDNA, clone SSG777.         44   6e-10  3
(AU039142) Dictyostelium discoideum slug cDNA, clone SSM440.       44   6e-10  3
(BJ408011) Dictyostelium discoideum cDNA clone:dds44l07, 3' ...    44   6e-10  3
(BJ393308) Dictyostelium discoideum cDNA clone:dds30j23, 5' ...    44   6e-10  3
(C91540) Dictyostelium discoideum slug cDNA, clone SSK414.         44   6e-10  3
(C90437) Dictyostelium discoideum slug cDNA, clone SSI495.         44   6e-10  3
(C93878) Dictyostelium discoideum slug cDNA, clone SSL820.         44   6e-10  3
(C91268) Dictyostelium discoideum slug cDNA, clone SSJ728.         44   6e-10  3
(C92129) Dictyostelium discoideum slug cDNA, clone SSD125.         44   6e-10  3
(C94261) Dictyostelium discoideum slug cDNA, clone SSK635.         44   6e-10  3
(BJ405189) Dictyostelium discoideum cDNA clone:dds30j23, 3' ...    44   6e-10  3
(AU074743) Dictyostelium discoideum slug cDNA, clone SSL723.       44   6e-10  3
(AU074352) Dictyostelium discoideum slug cDNA, clone SSK459.       44   6e-10  3
(AU074202) Dictyostelium discoideum slug cDNA, clone SSJ788.       44   6e-10  3
(AU074098) Dictyostelium discoideum slug cDNA, clone SSJ512.       44   6e-10  3
(AU074090) Dictyostelium discoideum slug cDNA, clone SSJ494.       44   6e-10  3
(AU074011) Dictyostelium discoideum slug cDNA, clone SSJ246.       44   6e-10  3
(AU073144) Dictyostelium discoideum slug cDNA, clone SSG250.       44   6e-10  3
(AU072248) Dictyostelium discoideum slug cDNA, clone SSD830.       44   6e-10  3
(AU072190) Dictyostelium discoideum slug cDNA, clone SSD629.       44   6e-10  3
(AU074211) Dictyostelium discoideum slug cDNA, clone SSJ814.       42   6e-10  3
(BJ405158) Dictyostelium discoideum cDNA clone:dds30d21, 3' ...    44   6e-10  3
(BJ402594) Dictyostelium discoideum cDNA clone:dds17n11, 3' ...    38   9e-10  4
(BJ397124) Dictyostelium discoideum cDNA clone:dds45l01, 5' ...    42   2e-09  3
(BJ368858) Dictyostelium discoideum cDNA clone:ddc48h09, 5' ...    48   2e-09  3
(BJ395327) Dictyostelium discoideum cDNA clone:dds38p12, 5' ...    48   2e-09  3
(C92770) Dictyostelium discoideum slug cDNA, clone SSF325.         42   2e-09  3
(AU037132) Dictyostelium discoideum slug cDNA, clone SSB513.       42   2e-09  3
(C92678) Dictyostelium discoideum slug cDNA, clone SSF823.         42   2e-09  3
(BJ408233) Dictyostelium discoideum cDNA clone:dds45l01, 3' ...    42   2e-09  3
(BJ384121) Dictyostelium discoideum cDNA clone:ddc48h09, 3' ...    48   2e-09  3
(AU038186) Dictyostelium discoideum slug cDNA, clone SSH384.       48   2e-09  3
(AU075015) Dictyostelium discoideum slug cDNA, clone SSM732.       48   2e-09  3
(C90373) Dictyostelium discoideum slug cDNA, clone SSI321.         44   3e-08  3
(AU037586) Dictyostelium discoideum slug cDNA, clone SSD821.       44   4e-07  3
(AU072652) Dictyostelium discoideum slug cDNA, clone SSA465.       34   2e-06  3
(C84218) Dictyostelium discoideum slug cDNA, clone SSB328.         44   2e-06  3
(AU074602) Dictyostelium discoideum slug cDNA, clone SSL349.       44   2e-06  3
(AU071976) Dictyostelium discoideum slug cDNA, clone SSC882.       44   3e-06  3
(C90896) Dictyostelium discoideum slug cDNA, clone SSJ280.         36   3e-06  4
(C25644) Dictyostelium discoideum slug cDNA, clone SSA848.         44   4e-06  3
(AU076393) Dictyostelium discoideum slug cDNA, clone SSI685.       44   4e-06  3
(AU074976) Dictyostelium discoideum slug cDNA, clone SSM630.       44   1e-05  2
(AU072351) Dictyostelium discoideum slug cDNA, clone SSE264.       44   7e-05  2
(AU071786) Dictyostelium discoideum slug cDNA, clone SSC521.       44   7e-05  2
(AU074764) Dictyostelium discoideum slug cDNA, clone SSL794.       48   0.002  2
(AM448606) Vitis vinifera contig VV78X241120.2, whole genome...    42   0.003  2
(AC015587) Homo sapiens clone RP11-44L13, LOW-PASS SEQUENCE ...    54   0.004  1
(C91419) Dictyostelium discoideum slug cDNA, clone SSK226.         46   0.009  2
(BX649314) Zebrafish DNA sequence from clone DKEYP-111A3 in ...    40   0.012  4
(EA102713) Sequence 96 from patent US 7195870.                     36   0.012  2
(AX323620) Sequence 108 from Patent WO0192565.                     36   0.012  2
(AX277933) Sequence 96 from Patent WO0177375.                      36   0.012  2
(C84063) Dictyostelium discoideum slug cDNA, clone SSC585.         44   0.015  2
(CU184798) Zebrafish DNA sequence from clone CH211-100M16 in...    38   0.042  6
(AU038750) Dictyostelium discoideum slug cDNA, clone SSL434.       34   0.052  2
(AU073197) Dictyostelium discoideum slug cDNA, clone SSG351.       34   0.052  2
(AC141731) Apis mellifera clone CH224-58F22, WORKING DRAFT S...    38   0.062  3
(EG318822) MSAM302564_3044_1691 LCM-dissected maize inbred l...    50   0.062  1
(AU075118) Dictyostelium discoideum slug cDNA, clone SSA289.       50   0.062  1
(AU072139) Dictyostelium discoideum slug cDNA, clone SSD502.       50   0.062  1
(AU071749) Dictyostelium discoideum slug cDNA, clone SSC446.       50   0.062  1
(BJ392223) Dictyostelium discoideum cDNA clone:dds26h23, 5' ...    32   0.079  3
(EU352212) Aedes aegypti mitochondrion, complete genome.           44   0.11   3
(CS374641) Sequence 744 from Patent WO2006034879.                  32   0.15   4
(CS374369) Sequence 472 from Patent WO2006034879.                  32   0.15   4
(AP009642) Lotus japonicus genomic DNA, clone: LjB06P23, BM1...    34   0.15   2
(AC016174) Homo sapiens clone RP11-20K23, WORKING DRAFT SEQU...    36   0.19   5

>(BJ396538) Dictyostelium discoideum cDNA clone:dds43d06, 5' end,
           single read.
          Length = 345

 Score =  361 bits (182), Expect(3) = e-158
 Identities = 188/190 (98%)
 Strand = Plus / Plus


Query: 126 ttttgttggaacttctcaatcaaataataatgctgctcttttaacccttgatgcattagt 185
           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 126 ttttgttggaacttctcaatctaataataatgctgctcttttaacccttgatgcattagt 185


Query: 186 tattattcatccaatattagcagtatatgctcatgttgacctttaaactgtttatctaaa 245
           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 186 tattatccatccaatattagcagtatatgctcatgttgacctttaaactgtttatctaaa 245


Query: 246 aataccattcatttaataatataaaactattttaaactatttaaattaagttgttgtaca 305
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 246 aataccattcatttaataatataaaactattttaaactatttaaattaagttgttgtaca 305


Query: 306 tttaaataaa 315
           ||||||||||
Sbjct: 306 tttaaataaa 315

 Score =  200 bits (101), Expect(3) = e-158
 Identities = 104/104 (100%)
 Strand = Plus / Plus


Query: 1   actgttggcctactggnaaaacacttgtattaaattgttaaaaatctaaaatgttattca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   actgttggcctactggnaaaacacttgtattaaattgttaaaaatctaaaatgttattca 60


Query: 61  aatcattaaattcaattgctaatatccaaagtgcatctcaaaag 104
           ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aatcattaaattcaattgctaatatccaaagtgcatctcaaaag 104

 Score = 48.1 bits (24), Expect(3) = e-158
 Identities = 24/24 (100%)
 Strand = Plus / Plus


Query: 105 aatcaaatttcaggaactcatttt 128
           ||||||||||||||||||||||||
Sbjct: 106 aatcaaatttcaggaactcatttt 129

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 508,292,068
Number of extensions: 34401253
Number of successful extensions: 3230344
Number of sequences better than 10.0: 348
Length of query: 414
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 391
Effective length of database: 93,106,754,628
Effective search space: 36404741059548
Effective search space used: 36404741059548
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

Query= Contig-U14647-1 (Contig-U14647-1Q) /CSM_Contig/Contig-U14647-1Q.Seq.d
         (414 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 360,445,970
Number of extensions: 4181142
Number of successful extensions: 3884
Number of sequences better than 10.0: 0
Number of HSP's gapped: 3884
Number of HSP's successfully gapped: 0
Length of query: 138
Length of database: 1,051,180,864
Length adjustment: 102
Effective length of query: 36
Effective length of database: 721,051,846
Effective search space: 25957866456
Effective search space used: 25957866456
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 29 (15.8 bits)

 psg: 0.54  gvh: 0.56  alm: 0.26  top: 0.60  tms: 0.07  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.22  leu: 0.22  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U14647-1 is nuc