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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U14300-1
Contig ID Contig-U14300-1
Contig update 2002.12.18
Contig sequence 
>Contig-U14300-1 (Contig-U14300-1Q) /CSM_Contig/Contig-U14300-1Q.Seq.d
NNNNNNNNNNATAGNCAGCCACATCAACANCAACANCAACAACAACAACA
ACAGCAACAACCTTTTAACAGACAGCCATCTCAACCATACCAACCACCTC
CACAACAACAACAACAACAACAACAACAACAACAACAACAACAAAAAAAA
TCAAGCGGATTTGAAAATATATTTGGTGATTTTGATTTTACCTCTCCTTC
ACAACAAAGACAGAACCCACAACCGCCTCAACAACAAAGTAATTATTTTA
ATAATGGTATGCCACCACAATCATCAGTTTCTCAACATGTTGATAATCAC
CAACATGAAAGATCAAATGTTGAACCTATAGTATCAGAGAAAATAAAAAT
GTGGGGTGAAAAGGGTGGTAGAAAGAATAATTTAAGAGTTTTATTATCGA
CACTTCATGAGGTTTTATGGATAGAAAGTGGTTGGGAAAAAGTAACCT
Gap no gap
Contig length 448
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 6959945
End point 6960383
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U14300
List of clone(s) 

est1=CHF439Z,1,439
Translated Amino Acid sequence 
XXXXXQPHQXQXQQQQQQQQPFNRQPSQPYQPPPQQQQQQQQQQQQQQKKSSGFENIFGD
FDFTSPSQQRQNPQPPQQQSNYFNNGMPPQSSVSQHVDNHQHERSNVEPIVSEKIKMWGE
KGGRKNNLRVLLSTLHEVLWIESGWEKVT
Translated Amino Acid sequence (All Frames) 
Frame A:
XXXXXQPHQXQXQQQQQQQQPFNRQPSQPYQPPPQQQQQQQQQQQQQQKKSSGFENIFGD
FDFTSPSQQRQNPQPPQQQSNYFNNGMPPQSSVSQHVDNHQHERSNVEPIVSEKIKMWGE
KGGRKNNLRVLLSTLHEVLWIESGWEKVT


Frame B:
xxxixshinxnxnnnnnsnnlltdshlnhtnhlhnnnnnnnnnnnnnkknqadlkiylvi
lilpllhnkdrthnrlnnkviilimvchhnhqflnmliitnmkdqmlnl*yqrk*kcgvk
rvverii*efyyrhfmrfyg*kvvgkk*p


Frame C:
xxx*xatstxtxtttttattf*qtaistipttsttttttttttttttkkikri*kyiw*f
*fylsfttktepttasttk*lf**wyattiisfstc**spt*kikc*tysirenknvg*k
gw*ke*fksfiidts*gfmdrkwlgksn
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U14300-1 (Contig-U14300-1Q)
/CSM_Contig/Contig-U14300-1Q.Seq.d
         (448 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U14300-1 (Contig-U14300-1Q) /CSM_Contig/Conti...   591  e-169
Contig-U10294-1 (Contig-U10294-1Q) /CSM_Contig/Conti...    38  0.008
Contig-U14399-1 (Contig-U14399-1Q) /CSM_Contig/Conti...    34  0.12
Contig-U11864-1 (Contig-U11864-1Q) /CSM_Contig/Conti...    34  0.12
Contig-U08544-1 (Contig-U08544-1Q) /CSM_Contig/Conti...    34  0.12
Contig-U03832-1 (Contig-U03832-1Q) /CSM_Contig/Conti...    34  0.12
Contig-U13653-1 (Contig-U13653-1Q) /CSM_Contig/Conti...    32  0.48
Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Conti...    32  0.48
Contig-U12528-1 (Contig-U12528-1Q) /CSM_Contig/Conti...    32  0.48
Contig-U11174-1 (Contig-U11174-1Q) /CSM_Contig/Conti...    32  0.48

>Contig-U14300-1 (Contig-U14300-1Q) /CSM_Contig/Contig-U14300-1Q.Seq.d
          Length = 448

 Score =  591 bits (298), Expect = e-169
 Identities = 298/298 (100%)
 Strand = Plus / Plus

                                                                       
Query: 151 tcaagcggatttgaaaatatatttggtgattttgattttacctctccttcacaacaaaga 210
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 151 tcaagcggatttgaaaatatatttggtgattttgattttacctctccttcacaacaaaga 210

                                                                       
Query: 211 cagaacccacaaccgcctcaacaacaaagtaattattttaataatggtatgccaccacaa 270
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 211 cagaacccacaaccgcctcaacaacaaagtaattattttaataatggtatgccaccacaa 270

                                                                       
Query: 271 tcatcagtttctcaacatgttgataatcaccaacatgaaagatcaaatgttgaacctata 330
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 271 tcatcagtttctcaacatgttgataatcaccaacatgaaagatcaaatgttgaacctata 330

                                                                       
Query: 331 gtatcagagaaaataaaaatgtggggtgaaaagggtggtagaaagaataatttaagagtt 390
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 331 gtatcagagaaaataaaaatgtggggtgaaaagggtggtagaaagaataatttaagagtt 390

                                                                     
Query: 391 ttattatcgacacttcatgaggttttatggatagaaagtggttgggaaaaagtaacct 448
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 391 ttattatcgacacttcatgaggttttatggatagaaagtggttgggaaaaagtaacct 448


 Score = 79.8 bits (40), Expect = 2e-15
 Identities = 40/40 (100%)
 Strand = Plus / Plus

                                                   
Query: 62  cttttaacagacagccatctcaaccataccaaccacctcc 101
           ||||||||||||||||||||||||||||||||||||||||
Sbjct: 62  cttttaacagacagccatctcaaccataccaaccacctcc 101


>Contig-U10294-1 (Contig-U10294-1Q) /CSM_Contig/Contig-U10294-1Q.Seq.d
          Length = 720

 Score = 38.2 bits (19), Expect = 0.008
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 270 atcatcagtttctcaacat 288
           |||||||||||||||||||
Sbjct: 355 atcatcagtttctcaacat 373


>Contig-U14399-1 (Contig-U14399-1Q) /CSM_Contig/Contig-U14399-1Q.Seq.d
          Length = 1289

 Score = 34.2 bits (17), Expect = 0.12
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                             
Query: 365  gtggtagaaagaataat 381
            |||||||||||||||||
Sbjct: 1066 gtggtagaaagaataat 1082


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 6736
Number of Sequences: 6905
Number of extensions: 6736
Number of successful extensions: 614
Number of sequences better than 10.0: 163
length of query: 448
length of database: 5,674,871
effective HSP length: 16
effective length of query: 432
effective length of database: 5,564,391
effective search space: 2403816912
effective search space used: 2403816912
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.12.20
Homology vs DNA 
Query= Contig-U14300-1 (Contig-U14300-1Q) /CSM_Contig/Contig-U14300-1Q.Seq.d
         (448 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC114263) Dictyostelium discoideum chromosome 2 map 215673-...   591   e-165  1
(BJ378660) Dictyostelium discoideum cDNA clone:ddc32n10, 3' ...   591   e-165  1
(AC130511) Rattus norvegicus clone CH230-390A8, *** SEQUENCI...    40   0.008  4
(AC212894) MACACA MULATTA BAC clone CH250-6J16 from chromoso...    36   0.16   2
(AC212004) MACACA MULATTA BAC clone CH250-393D12 from chromo...    36   0.16   2
(CT025155) Mouse DNA sequence from clone RP23-228F18 on chro...    38   0.83   3
(EJ191158) 1092344199152 Global-Ocean-Sampling_GS-27-01-01-1...    46   1.1    1
(BC158797) Rattus norvegicus similar to receptor-interacting...    44   4.2    1
(BC099834) Rattus norvegicus similar to receptor-interacting...    44   4.2    1
(AP008210) Oryza sativa (japonica cultivar-group) genomic DN...    44   4.2    1
(AP008209) Oryza sativa (japonica cultivar-group) genomic DN...    44   4.2    1
(AL731614) Oryza sativa genomic DNA, chromosome 4, BAC clone...    44   4.2    1
(AC137991) Oryza sativa chromosome 3 BAC OSJNBa0034D21 genom...    44   4.2    1
(EA702783) Sequence 81662 from patent US 7365185.                  44   4.2    1
(EA680923) Sequence 59802 from patent US 7365185.                  44   4.2    1
(AC128975) Rattus norvegicus clone CH230-1A17, *** SEQUENCIN...    44   4.2    1
(AC105569) Rattus norvegicus clone CH230-231J21, *** SEQUENC...    44   4.2    1
(AL731625) Oryza sativa genomic DNA, chromosome 4, BAC clone...    44   4.2    1
(BC092366) Rattus norvegicus cDNA clone IMAGE:7319656, with ...    44   4.2    1
(CZ657268) OM__Ba0208C12.r OM__Ba Oryza minuta genomic clone...    44   4.2    1
(CA512408) UI-R-FJ0-cpz-n-03-0-UI.r1 UI-R-FJ0 Rattus norvegi...    44   4.2    1
(BM913313) AGENCOURT_6610392 NIH_MGC_41 Homo sapiens cDNA cl...    44   4.2    1
(W22242) 64A4 Human retina cDNA Tsp509I-cleaved sublibrary H...    44   4.2    1
(FE343350) CAOF4263.fwd CAOF_Daphnia_pulex_Log50_Library_12 ...    44   4.2    1
(EV768721) AGENCOURT_109871060 NIH_MGC_418 Rattus norvegicus...    44   4.2    1
(CF877959) tric077xp06.b1 T.reesei mycelial culture, Version...    36   4.6    2
(CB906174) tric077xp06 T.reesei mycelial culture, Version 3 ...    36   5.0    2
(EI926308) VUH2-67C23TV VUUBBa (VUH2) Vigna unguiculata geno...    34   6.0    2
(EJ536854) 1092955264726 Global-Ocean-Sampling_GS-29-01-01-1...    32   6.2    3
(CU862050) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    38   6.2    4
(AC171988) Bos taurus clone CH240-175B17, *** SEQUENCING IN ...    32   7.4    2
(CU570829) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    42   8.3    3
(AC034169) Homo sapiens chromosome 1 clone RP11-659J18, WORK...    36   8.6    4
(AC000055) Human PAC clone DJ073F11 from Xq23, complete sequ...    42   8.9    3
(BP640882) Arabidopsis thaliana cDNA clone:RAFL19-53-E22, 3'...    36   8.9    2
(AM482555) Vitis vinifera, whole genome shotgun sequence, co...    42   9.5    2

>(AC114263) Dictyostelium discoideum chromosome 2 map 215673-367476 strain
              AX4, complete sequence.
          Length = 151802

 Score =  591 bits (298), Expect = e-165
 Identities = 298/298 (100%)
 Strand = Plus / Plus


Query: 151    tcaagcggatttgaaaatatatttggtgattttgattttacctctccttcacaacaaaga 210
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116235 tcaagcggatttgaaaatatatttggtgattttgattttacctctccttcacaacaaaga 116294


Query: 211    cagaacccacaaccgcctcaacaacaaagtaattattttaataatggtatgccaccacaa 270
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116295 cagaacccacaaccgcctcaacaacaaagtaattattttaataatggtatgccaccacaa 116354


Query: 271    tcatcagtttctcaacatgttgataatcaccaacatgaaagatcaaatgttgaacctata 330
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116355 tcatcagtttctcaacatgttgataatcaccaacatgaaagatcaaatgttgaacctata 116414


Query: 331    gtatcagagaaaataaaaatgtggggtgaaaagggtggtagaaagaataatttaagagtt 390
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116415 gtatcagagaaaataaaaatgtggggtgaaaagggtggtagaaagaataatttaagagtt 116474


Query: 391    ttattatcgacacttcatgaggttttatggatagaaagtggttgggaaaaagtaacct 448
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116475 ttattatcgacacttcatgaggttttatggatagaaagtggttgggaaaaagtaacct 116532

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 434,265,721
Number of extensions: 28470492
Number of successful extensions: 2305393
Number of sequences better than 10.0: 36
Length of query: 448
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 425
Effective length of database: 93,106,754,628
Effective search space: 39570370716900
Effective search space used: 39570370716900
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 7.10
Homology vs Protein 
Query= Contig-U14300-1 (Contig-U14300-1Q) /CSM_Contig/Contig-U14300-1Q.Seq.d
         (448 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC114263_41(AC114263|pid:none) Dictyostelium discoideum chromoso...   176   2e-43
FN392322_776(FN392322|pid:none) Pichia pastoris GS115 chromosome...    47   3e-04
AY081334_1(AY081334|pid:none) Arabidopsis thaliana auxilin-like ...    47   3e-04
CR382128_479(CR382128|pid:none) Yarrowia lipolytica strain CLIB1...    47   3e-04
AK228848_1(AK228848|pid:none) Arabidopsis thaliana mRNA for auxi...    46   5e-04
AP008217_1417(AP008217|pid:none) Oryza sativa (japonica cultivar...    44   0.001
AK100317_1(AK100317|pid:none) Oryza sativa Japonica Group cDNA c...    44   0.002
AC141112_7(AC141112|pid:none) Medicago truncatula clone mth2-15m...    44   0.002
EU974649_1(EU974649|pid:none) Zea mays clone 455133 hypothetical...    43   0.004
AK071995_1(AK071995|pid:none) Oryza sativa Japonica Group cDNA c...    42   0.005
AL513444_22(AL513444|pid:none) Neurospora crassa DNA linkage gro...    42   0.005
AC025814_1(AC025814|pid:none) Arabidopsis thaliana chromosome 1 ...    42   0.009
AC023754_14(AC023754|pid:none) Arabidopsis thaliana chromosome I...    42   0.009
AC073506_9(AC073506|pid:none) Arabidopsis thaliana chromosome 1 ...    42   0.009
CU640366_967(CU640366|pid:none) Podospora anserina genomic DNA c...    40   0.026
AP007167_502(AP007167|pid:none) Aspergillus oryzae RIB40 genomic...    40   0.026
AP008211_2051(AP008211|pid:none) Oryza sativa (japonica cultivar...    39   0.044
AC007858_11(AC007858|pid:none) Oryza sativa (japonica cultivar-g...    39   0.044
AP004317_15(AP004317|pid:none) Oryza sativa Japonica Group genom...    39   0.075
AY231908_1(AY231908|pid:none) Drosophila yakuba clone yak-em_CG1...    38   0.098
AE014297_10(AE014297|pid:none) Drosophila melanogaster chromosom...    37   0.28
AY069440_1(AY069440|pid:none) Drosophila melanogaster LD15107 fu...    37   0.28
AL139294_3(AL139294|pid:none) Human DNA sequence from clone RP5-...    35   0.83
(O75061) RecName: Full=Putative tyrosine-protein phosphatase aux...    35   0.83
AK147570_1(AK147570|pid:none) Mus musculus adult male brain UNDE...    35   0.83
AB007942_1(AB007942|pid:none) Homo sapiens mRNA for KIAA0473 pro...    35   0.83
AK122293_1(AK122293|pid:none) Mus musculus mRNA for mKIAA0473 pr...    35   0.83
EU339283_1(EU339283|pid:none) Sus scrofa DnaJ-like protein subfa...    35   0.83
(Q27974) RecName: Full=Putative tyrosine-protein phosphatase aux...    35   0.83
AL096860_2(AL096860|pid:none) Arabidopsis thaliana DNA chromosom...    35   1.1
AK034637_1(AK034637|pid:none) Mus musculus 12 days embryo embryo...    35   1.1
(Q99KY4) RecName: Full=Cyclin G-associated kinase;          EC=2...    35   1.1
A86453(A86453) CDS protein F9L11.3 [imported] - Arabidopsis thal...    35   1.1
AC007197_8(AC007197|pid:none) Arabidopsis thaliana chromosome 2 ...    35   1.1
AK146695_1(AK146695|pid:none) Mus musculus 17 days pregnant adul...    35   1.1
AK166682_1(AK166682|pid:none) Mus musculus blastocyst blastocyst...    35   1.1
AF096371_1(AF096371|pid:none) Arabidopsis thaliana BAC T2L5.  &T...    35   1.1
BC030859_1(BC030859|pid:none) Mus musculus cyclin G associated k...    35   1.1
AK126735_1(AK126735|pid:none) Homo sapiens cDNA FLJ44781 fis, cl...    34   1.4
AM910995_366(AM910995|pid:none) Plasmodium knowlesi strain H chr...    34   1.4
BC085005_1(BC085005|pid:none) Homo sapiens cyclin G associated k...    34   1.4
BT007748_1(BT007748|pid:none) Synthetic construct Homo sapiens c...    34   1.4
BC008668_1(BC008668|pid:none) Homo sapiens cyclin G associated k...    34   1.4
BC063606_1(BC063606|pid:none) Homo sapiens cyclin G associated k...    34   1.4
AB208854_1(AB208854|pid:none) Homo sapiens mRNA for Cyclin G-ass...    34   1.4
BC000816_1(BC000816|pid:none) Homo sapiens, clone IMAGE:3453673,...    34   1.4
D88435_1(D88435|pid:none) Homo sapiens mRNA for HsGAK, complete ...    34   1.4
(Q5UNU5) RecName: Full=Uncharacterized protein L684;  &AY653733_...    33   2.4
BC105343_1(BC105343|pid:none) Bos taurus cyclin G associated kin...    33   3.1
CR857994_1(CR857994|pid:none) Pongo abelii mRNA; cDNA DKFZp469F1...    33   3.1
AC007211_9(AC007211|pid:none) Arabidopsis thaliana chromosome 2 ...    32   5.4
AE014187_108(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    32   5.4
CY005719_1(CY005719|pid:none) Influenza A virus (A/sooty tern/We...    32   9.1
(Q8IRJ8) RecName: Full=THO complex protein 7;  &AE014296_45(AE01...    32   9.1
BT024970_1(BT024970|pid:none) Drosophila melanogaster IP15218 fu...    32   9.1
CR954203_563(CR954203|pid:none) Ostreococcus tauri strain OTTH05...    32   9.1
CY005407_1(CY005407|pid:none) Influenza A virus (A/wedge-tailed ...    32   9.1

>AC114263_41(AC114263|pid:none) Dictyostelium discoideum chromosome
           2 map 215673-367476 strain AX4, complete sequence.
          Length = 730

 Score =  176 bits (446), Expect = 2e-43
 Identities = 91/144 (63%), Positives = 91/144 (63%)
 Frame = +1

Query: 16  QPHXXXXXXXXXXXXPFNRXXXXXXXXXXXXXXXXXXXXXXXXKKSSGFENIFGDFDFTX 195
           QPH            PFNR                        KKSSGFENIFGDFDFT
Sbjct: 528 QPHQQQQQQQQQQQQPFNRQPSQPYQPPPQQQQQQQQQQQQQQKKSSGFENIFGDFDFTS 587

Query: 196 XXXXXXXXXXXXXXXXYFNNGMPPQSSVSQHVDNHQHERSNVEPIVSEKIKMWGEKGGRK 375
                           YFNNGMPPQSSVSQHVDNHQHERSNVEPIVSEKIKMWGEKGGRK
Sbjct: 588 PSQQRQNPQPPQQQSNYFNNGMPPQSSVSQHVDNHQHERSNVEPIVSEKIKMWGEKGGRK 647

Query: 376 NNLRVLLSTLHEVLWIESGWEKVT 447
           NNLRVLLSTLHEVLWIESGWEKVT
Sbjct: 648 NNLRVLLSTLHEVLWIESGWEKVT 671

Lambda     K      H
   0.315    0.133    0.418

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 493,799,520
Number of extensions: 8218447
Number of successful extensions: 17713
Number of sequences better than 10.0: 57
Number of HSP's gapped: 17709
Number of HSP's successfully gapped: 57
Length of query: 149
Length of database: 1,051,180,864
Length adjustment: 111
Effective length of query: 38
Effective length of database: 691,922,815
Effective search space: 26293066970
Effective search space used: 26293066970
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 29 (15.8 bits)
PSORT 

 psg: 0.55  gvh: 0.28  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for Contig-U14300-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0