Contig-U02566-1
Contig ID Contig-U02566-1
Contig update 2002. 9.13
Contig sequence
>Contig-U02566-1 (Contig-U02566-1Q) /CSM_Contig/Contig-U02566-1Q.Seq.d
CCACTGCCAACACCAACACCAACACCAACACCACAACCACAGGAACATCA
ACATCAACAAATAATCAATCACCATTTTTAACGAAATTACAGCAACAACA
AAAGGTAAAAG

Gap no gap
Contig length 111
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 7759614
End point 7759725
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U02566
List of clone(s)

est1=AFE102Z,1,112
Translated Amino Acid sequence
TANTNTNTNTTTTGTSTSTNNQSPFLTKLQQQQKVK


Translated Amino Acid sequence (All Frames)
Frame A:
plptptptptpqpqehqhqqiinhhf*rnysnnkr*k


Frame B:
hcqhqhqhqhhnhrninink*sitifneitattkgk


Frame C:
TANTNTNTNTTTTGTSTSTNNQSPFLTKLQQQQKVK


own update 2004. 6. 9
Homology vs CSM-cDNA
Query= Contig-U02566-1 (Contig-U02566-1Q)
/CSM_Contig/Contig-U02566-1Q.Seq.d
(111 letters)

Database: CSM
6905 sequences; 5,674,871 total letters


Score E
Sequences producing significant alignments: (bits) Value

Contig-U02566-1 (Contig-U02566-1Q) /CSM_Contig/Conti... 101 1e-22
Contig-U11472-1 (Contig-U11472-1Q) /CSM_Contig/Conti... 36 0.007
Contig-U11360-1 (Contig-U11360-1Q) /CSM_Contig/Conti... 36 0.007
Contig-U00886-1 (Contig-U00886-1Q) /CSM_Contig/Conti... 36 0.007
Contig-U12710-1 (Contig-U12710-1Q) /CSM_Contig/Conti... 34 0.028
Contig-U11376-1 (Contig-U11376-1Q) /CSM_Contig/Conti... 34 0.028
Contig-U11235-1 (Contig-U11235-1Q) /CSM_Contig/Conti... 34 0.028
Contig-U09732-1 (Contig-U09732-1Q) /CSM_Contig/Conti... 34 0.028
Contig-U11864-1 (Contig-U11864-1Q) /CSM_Contig/Conti... 32 0.11
Contig-U11683-1 (Contig-U11683-1Q) /CSM_Contig/Conti... 32 0.11

>Contig-U02566-1 (Contig-U02566-1Q) /CSM_Contig/Contig-U02566-1Q.Seq.d
Length = 111

Score = 101 bits (51), Expect = 1e-22
Identities = 51/51 (100%)
Strand = Plus / Plus


Query: 61 ataatcaatcaccatttttaacgaaattacagcaacaacaaaaggtaaaag 111
|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61 ataatcaatcaccatttttaacgaaattacagcaacaacaaaaggtaaaag 111


>Contig-U11472-1 (Contig-U11472-1Q) /CSM_Contig/Contig-U11472-1Q.Seq.d
Length = 3643

Score = 36.2 bits (18), Expect = 0.007
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 89 acagcaacaacaaaaggtaaaa 110
|||||||||||||||| |||||
Sbjct: 2108 acagcaacaacaaaagataaaa 2129


>Contig-U11360-1 (Contig-U11360-1Q) /CSM_Contig/Contig-U11360-1Q.Seq.d
Length = 3470

Score = 36.2 bits (18), Expect = 0.007
Identities = 18/18 (100%)
Strand = Plus / Plus


Query: 61 ataatcaatcaccatttt 78
||||||||||||||||||
Sbjct: 1529 ataatcaatcaccatttt 1546


Database: CSM
Posted date: Jun 9, 2004 7:35 PM
Number of letters in database: 5,674,871
Number of sequences in database: 6905

Lambda K H
1.37 0.711 1.31

Gapped
Lambda K H
1.37 0.711 1.31


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1449
Number of Sequences: 6905
Number of extensions: 1449
Number of successful extensions: 565
Number of sequences better than 10.0: 249
length of query: 111
length of database: 5,674,871
effective HSP length: 14
effective length of query: 97
effective length of database: 5,578,201
effective search space: 541085497
effective search space used: 541085497
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 13 (26.3 bits)
dna update 2009. 5.18
Homology vs DNA
Query= Contig-U02566-1 (Contig-U02566-1Q) /CSM_Contig/Contig-U02566-1Q.Seq.d
(111 letters)

Database: ddbj_A
102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

Score E
Sequences producing significant alignments: (bits) Value N

(AC116977) Dictyostelium discoideum chromosome 2 map 5515173... 74 1e-09 1
(BJ341980) Dictyostelium discoideum cDNA clone:dda9c01, 3' e... 74 1e-09 1
(AF506024) Tribolium castaneum isolate pGPUb3 polyubiquitin ... 44 0.94 1
(DT772423) 125606589 TH1 Tribolium castaneum cDNA clone 25M7... 44 0.94 1
(ES553373) TO1010A07pTV TO1 Tribolium castaneum cDNA, mRNA s... 44 0.94 1
(ES545331) TB1006D09pTV TB1 Tribolium castaneum cDNA, mRNA s... 44 0.94 1
(FP017240) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f... 42 3.7 1
(AC232954) Microcebus murinus clone CH257-205K5, WORKING DRA... 42 3.7 1
(AC199674) Lemur catta clone LB2-211K9, WORKING DRAFT SEQUEN... 42 3.7 1
(AC184727) Microcebus murinus clone CH257-521D18, WORKING DR... 42 3.7 1
(AC171880) Bos taurus clone CH240-279A5, WORKING DRAFT SEQUE... 42 3.7 1
(AC167427) Bos taurus clone CH240-210L2, WORKING DRAFT SEQUE... 42 3.7 1
(CL409733) RPCI44_417B22.r RPCI-44 Sus scrofa genomic clone ... 42 3.7 1
(EG628233) EMBRYOF101125O10 POSSUM_01-POSSUM-C-EMBRYO-2KB Tr... 42 3.7 1
(EG624142) EMBRYOF095453K19 POSSUM_01-POSSUM-C-EMBRYO-2KB Tr... 42 3.7 1
(DY613010) IMMUNEF078647L21 POSSUM_01-C-POSSUM-IMMUNE-2KB Tr... 42 3.7 1
(CR543861) Acinetobacter sp. ADP1 complete genome. 42 3.7 1

>(AC116977) Dictyostelium discoideum chromosome 2 map 5515173-5817331
strain AX4, complete sequence.
Length = 302156

Score = 73.8 bits (37), Expect = 1e-09
Identities = 37/37 (100%)
Strand = Plus / Plus


Query: 75 tttttaacgaaattacagcaacaacaaaaggtaaaag 111
|||||||||||||||||||||||||||||||||||||
Sbjct: 167178 tttttaacgaaattacagcaacaacaaaaggtaaaag 167214

Lambda K H
1.37 0.711 1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 43,087,372
Number of extensions: 3885093
Number of successful extensions: 1038909
Number of sequences better than 10.0: 17
Length of query: 111
Length of database: 101,790,757,118
Length adjustment: 22
Effective length of query: 89
Effective length of database: 99,544,435,898
Effective search space: 8859454794922
Effective search space used: 8859454794922
X1: 11 (21.8 bits)
S2: 20 (40.1 bits)

protein update 2009. 7.19
Homology vs Protein
Query= Contig-U02566-1 (Contig-U02566-1Q) /CSM_Contig/Contig-U02566-1Q.Seq.d
(111 letters)

Database: nrp_B
3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

Score E
Sequences producing significant alignments: (bits) Value

AC116977_64(AC116977|pid:none) Dictyostelium discoideum chromoso... 33 3.2

>AC116977_64(AC116977|pid:none) Dictyostelium discoideum chromosome
2 map 5515173-5817331 strain AX4, complete sequence.
Length = 914

Score = 33.1 bits (74), Expect = 3.2
Identities = 15/15 (100%), Positives = 15/15 (100%)
Frame = +3

Query: 66 QSPFLTKLQQQQKVK 110
QSPFLTKLQQQQKVK
Sbjct: 479 QSPFLTKLQQQQKVK 493

Lambda K H
0.318 0.134 0.401

Gapped
Lambda K H
0.267 0.0410 0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 60,769,656
Number of extensions: 377499
Number of successful extensions: 435
Number of sequences better than 10.0: 1
Number of HSP's gapped: 428
Number of HSP's successfully gapped: 1
Length of query: 37
Length of database: 1,051,180,864
Length adjustment: 11
Effective length of query: 26
Effective length of database: 1,015,578,715
Effective search space: 26405046590
Effective search space used: 26405046590
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 24 (13.9 bits)

PSORT

psg: 0.85 gvh: 0.28 alm: 0.71 top: 0.67 tms: 0.00 mit: 0.50 mip: 0.00
nuc: 0.00 erl: 0.00 erm: 0.20 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00
act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00
dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00
m3a: 0.00 m3b: 0.00 m_ : 1.00

52.0 %: mitochondrial
32.0 %: nuclear
8.0 %: cytoskeletal
8.0 %: cytoplasmic

>> prediction for Contig-U02566-1 is mit

VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0