>Contig-U14102-1 (Contig-U14102-1Q) /CSM_Contig/Contig-U14102-1Q.Seq.d
AAAACGCAACTTTGTACACATAAAATCACACGCTCAAAACCCATATAAAT
TATTTAATTTAAACCATAATTTTTAATAATTTTTTTTTTTTTCTCTATTA
ATTAAATAATTTTAACAATACACACATAATGTCCGAATCACAATTAAAAA
AAGTTTTAAAAGAAAATGAAACCTTAAAAGCTCAATTAGAAAAATCAACT
ACCATCTTAAAAGTTTCTGAAGCATGTGAAAGTTTACAAGATTATTGTAC
CAAAACAAGTGATCCATTTATCCCAGGTTGGTCAGGTGAAAATGAATGGA
CTAAACCACTCAAAGGAAATGGATGTTCTGTGTTATAAATATTATCAACT
TATACATTGTACATTATATAGAATTTAAACAAACAAATAAAGTAATAGTA
ATAATAATAAAACAAACAAATAAAAAAATAAAATAAAAAAAACAAAAAAA
AAAAATAAAAAAAAAAAAAAAAAATAAAAAAGGTTAAAATTAATTAGCTC
AAAAAA

est1=AHK530E,1,438
est2=VSB796E,22,507
est3=VSK473F,32,357
est4=VSK473Z,34,354
est5=VSG774F,262,480

natlyt*nhtlkthinyli*tiifnnffffly*LNNFNNTHIMSESQLKKVLKENETLKA
QLEKSTTILKVSEACESLQDYCTKTSDPFIPGWSGENEWTKPLKGNGCSVL*ilstytly
ii*nlnkqik******nkqikk*nkknkkkk*kkkkkikkvkin*lkk

Frame A:
ktqlcthkitrskpi*ii*fkp*fliiffffsin*iiltiht*cpnhn*kkf*kkmkp*k
ln*knqlps*kflkhvkvykiivpkqvihlsqvgqvkmnglnhskemdvlcykyyqlihc
tlyri*tnk*snsnnnktnk*knkikktkkknkkkkkk*krlklissk


Frame B:
krnfvhikshaqnpyklfnlnhnf**ffffsllik*f*qythnvritikksfkrk*nlks
sirkinyhlksf*sm*kftrllyqnk*siyprlvr*k*md*ttqrkwmfcviniinlyiv
hyiefkqtnkviviiikqtnkkik*kkqkkkikkkkknkkg*n*laqk


Frame C:
natlyt*nhtlkthinyli*tiifnnffffly*LNNFNNTHIMSESQLKKVLKENETLKA
QLEKSTTILKVSEACESLQDYCTKTSDPFIPGWSGENEWTKPLKGNGCSVL*ilstytly
ii*nlnkqik******nkqikk*nkknkkkk*kkkkkikkvkin*lkk

Query= Contig-U14102-1 (Contig-U14102-1Q)
/CSM_Contig/Contig-U14102-1Q.Seq.d
         (506 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U14102-1 (Contig-U14102-1Q) /CSM_Contig/Conti...   519  e-148
Contig-U11906-1 (Contig-U11906-1Q) /CSM_Contig/Conti...    36  0.035
Contig-U08786-1 (Contig-U08786-1Q) /CSM_Contig/Conti...    36  0.035
Contig-U06408-1 (Contig-U06408-1Q) /CSM_Contig/Conti...    36  0.035
Contig-U13840-1 (Contig-U13840-1Q) /CSM_Contig/Conti...    34  0.14
Contig-U09433-1 (Contig-U09433-1Q) /CSM_Contig/Conti...    34  0.14
Contig-U09282-1 (Contig-U09282-1Q) /CSM_Contig/Conti...    34  0.14
Contig-U06106-1 (Contig-U06106-1Q) /CSM_Contig/Conti...    34  0.14
Contig-U05948-1 (Contig-U05948-1Q) /CSM_Contig/Conti...    34  0.14
Contig-U04159-1 (Contig-U04159-1Q) /CSM_Contig/Conti...    34  0.14

>Contig-U14102-1 (Contig-U14102-1Q) /CSM_Contig/Contig-U14102-1Q.Seq.d
          Length = 506

 Score =  519 bits (262), Expect = e-148
 Identities = 276/283 (97%)
 Strand = Plus / Plus

                                                                       
Query: 93  ctctattaattaaataattttaacaatacacacataatgtccgaatcacaattnnnnnnn 152
           |||||||||||||||||||||||||||||||||||||||||||||||||||||       
Sbjct: 93  ctctattaattaaataattttaacaatacacacataatgtccgaatcacaattaaaaaaa 152

                                                                       
Query: 153 gttttaaaagaaaatgaaaccttaaaagctcaattagaaaaatcaactaccatcttaaaa 212
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 153 gttttaaaagaaaatgaaaccttaaaagctcaattagaaaaatcaactaccatcttaaaa 212

                                                                       
Query: 213 gtttctgaagcatgtgaaagtttacaagattattgtaccaaaacaagtgatccatttatc 272
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 213 gtttctgaagcatgtgaaagtttacaagattattgtaccaaaacaagtgatccatttatc 272

                                                                       
Query: 273 ccaggttggtcaggtgaaaatgaatggactaaaccactcaaaggaaatggatgttctgtg 332
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 273 ccaggttggtcaggtgaaaatgaatggactaaaccactcaaaggaaatggatgttctgtg 332

                                                      
Query: 333 ttataaatattatcaacttatacattgtacattatatagaatt 375
           |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 333 ttataaatattatcaacttatacattgtacattatatagaatt 375


 Score =  157 bits (79), Expect = 1e-38
 Identities = 79/79 (100%)
 Strand = Plus / Plus

                                                                      
Query: 1  aaaacgcaactttgtacacataaaatcacacgctcaaaacccatataaattatttaattt 60
          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  aaaacgcaactttgtacacataaaatcacacgctcaaaacccatataaattatttaattt 60

                             
Query: 61 aaaccataatttttaataa 79
          |||||||||||||||||||
Sbjct: 61 aaaccataatttttaataa 79


 Score = 50.1 bits (25), Expect = 2e-06
 Identities = 25/25 (100%)
 Strand = Plus / Plus

                                    
Query: 482 ggttaaaattaattagctcaaaaaa 506
           |||||||||||||||||||||||||
Sbjct: 482 ggttaaaattaattagctcaaaaaa 506


>Contig-U11906-1 (Contig-U11906-1Q) /CSM_Contig/Contig-U11906-1Q.Seq.d
          Length = 2132

 Score = 36.2 bits (18), Expect = 0.035
 Identities = 18/18 (100%)
 Strand = Plus / Minus

                             
Query: 44  tataaattatttaattta 61
           ||||||||||||||||||
Sbjct: 136 tataaattatttaattta 119


>Contig-U08786-1 (Contig-U08786-1Q) /CSM_Contig/Contig-U08786-1Q.Seq.d
          Length = 1161

 Score = 36.2 bits (18), Expect = 0.035
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 350 ttatacattgtacattat 367
           ||||||||||||||||||
Sbjct: 254 ttatacattgtacattat 271


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8443
Number of Sequences: 6905
Number of extensions: 8443
Number of successful extensions: 1083
Number of sequences better than 10.0: 303
length of query: 506
length of database: 5,674,871
effective HSP length: 16
effective length of query: 490
effective length of database: 5,564,391
effective search space: 2726551590
effective search space used: 2726551590
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)

Query= Contig-U14102-1 (Contig-U14102-1Q) /CSM_Contig/Contig-U14102-1Q.Seq.d
         (506 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ354059) Dictyostelium discoideum cDNA clone:dda52k07, 3' ...   359   e-157  3
(AU262254) Dictyostelium discoideum vegetative cDNA clone:VS...   359   e-146  3
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   287   e-142  4
(BJ336009) Dictyostelium discoideum cDNA clone:dda52k07, 5' ...   250   e-123  3
(AU271258) Dictyostelium discoideum vegetative cDNA clone:VS...   250   e-106  3
(AJ312388) Dictyostelium discoideum mRNA for G protein gamma...   297   2e-82  2
(AU271259) Dictyostelium discoideum vegetative cDNA clone:VS...   206   1e-70  3
(BJ342822) Dictyostelium discoideum cDNA clone:dda15a06, 3' ...   264   3e-66  1
(AU266802) Dictyostelium discoideum vegetative cDNA clone:VS...   163   7e-36  1
(DQ022283) Isoptronotum sp. CXX-2005 16S ribosomal RNA gene,...    30   0.016  3
(EV416085) 210749206 NGP Nasonia giraulti cDNA clone 130M7 3...    40   0.037  2
(AC207706) Glycine tomentella clone gtd1-114o24, complete se...    50   0.077  1
(AC187163) Glycine tomentella clone gtd1-144d1, complete seq...    50   0.077  1
(BM639019) 17000687567607 A.Gam.ad.cDNA1 Anopheles gambiae c...    38   0.24   2
(BM582766) 17000687276276 A.Gam.ad.cDNA.blood1 Anopheles gam...    38   0.25   2
(EG959195) AU_Ich_008D_A04 Whole organism normalized cDNA li...    34   0.26   3
(AM438983) Vitis vinifera contig VV78X043771.8, whole genome...    48   0.30   1
(ED734269) GM_WBb0092L13.r GM_WBb Glycine max genomic clone ...    32   0.59   3
(AL162419) Human DNA sequence from clone RP11-358M14 on chro...    36   0.65   2
(AB257240) Aplomya metallica mitochondrial gene for 16S rRNA...    34   0.84   2
(CU694656) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    36   1.1    3
(AC180810) Strongylocentrotus purpuratus clone R3-3071K12, W...    46   1.2    1
(FH215906) CHO_OF3265xn11f1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   1.2    1
(BZ047512) lki21e09.b1 B.oleracea002 Brassica oleracea genom...    46   1.2    1
(CR318595) Zebrafish DNA sequence from clone CH211-57B15 in ...    34   1.3    4
(AC111689) Rattus norvegicus clone CH230-66C23, *** SEQUENCI...    36   1.3    3
(ER531891) 1093015770509 Global-Ocean-Sampling_GS-35-01-01-1...    36   1.8    2
(AC113802) Rattus norvegicus clone CH230-293E22, *** SEQUENC...    42   2.0    2
(CU694629) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    34   2.4    2
(CU466446) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    32   2.4    2
(CR388191) Zebrafish DNA sequence from clone DKEY-110I10 in ...    32   2.4    2
(AL583829) Human DNA sequence from clone RP11-61E5 on chromo...    34   2.6    2
(BM608903) 17000687086872 A.Gam.ad.cDNA.blood1 Anopheles gam...    38   2.7    2
(AY123350) Silba adipata 16S ribosomal RNA gene, partial seq...    32   3.1    2
(CZ930841) 248614 Tomato EcoRI BAC Library Solanum lycopersi...    34   3.1    2
(EV420340) 210786840 NGP Nasonia giraulti cDNA clone 175G1 5...    34   3.1    2
(ED456573) AUAC-aal32a11.b1 Ascaris suum whole genome shotgu...    32   3.1    2
(EV415791) 210751989 NGP Nasonia giraulti cDNA clone 124O23 ...    34   3.1    2
(CE620238) tigr-gss-dog-17000313626707 Dog Library Canis lup...    34   3.2    2
(BM637536) 17000687564548 A.Gam.ad.cDNA1 Anopheles gambiae c...    34   3.3    2
(BM610376) 17000687110678 A.Gam.ad.cDNA.blood1 Anopheles gam...    34   3.4    2
(BZ011118) oed10f02.b1 B.oleracea002 Brassica oleracea genom...    32   4.5    3
(AC147250) Mus musculus BAC clone RP24-109P17 from 13, compl...    44   4.8    1
(AC105302) Mus musculus strain C57BL/6J clone rp23-45h18 map...    44   4.8    1
(AC090653) Mus musculus strain C57BL/6J chromosome 17 clone ...    44   4.8    1
(AP010319) Lotus japonicus genomic DNA, chromosome 2, clone:...    44   4.8    1
(AC128678) Homo sapiens 12 BAC RP11-81H3 (Roswell Park Cance...    44   4.8    1
(AC123295) Rattus norvegicus clone CH230-348I4, *** SEQUENCI...    44   4.8    1
(CU695279) Brassica rapa subsp. pekinensis clone KBrB124L09,...    44   4.8    1
(AP010088) Lotus japonicus genomic DNA, clone: LjT16I12, TM0...    44   4.8    1
(AC231469) Choloepus hoffmanni clone CH281-768I7, WORKING DR...    44   4.8    1
(AC231467) Choloepus hoffmanni clone CH281-451A24, WORKING D...    44   4.8    1
(DU115303) KBrH100O05F Brassica rapa BAC library KBrH Brassi...    44   4.8    1
(CZ929827) 247355 Tomato EcoRI BAC Library Solanum lycopersi...    44   4.8    1
(CL500988) SAIL_691_C06.v1 SAIL Collection Arabidopsis thali...    44   4.8    1
(CC228516) CH261-11G17_RM1.1 CH261 Gallus gallus genomic clo...    44   4.8    1
(AG278778) Mus musculus molossinus DNA, clone:MSMg01-049E04....    44   4.8    1
(BW588139) Ciona savignyi cDNA, clone:csef002o11, 5'end, sin...    44   4.8    1
(AM479507) Vitis vinifera contig VV78X180538.6, whole genome...    38   4.8    3
(AC166987) Mus musculus chromosome 7 clone RP24-330C5 map 7,...    38   5.0    3
(AC099582) Mus musculus chromosome 1, clone RP23-188D7, comp...    38   5.4    2
(CW724449) MARC_1179114 CHORI-240-123E2 Bos taurus genomic c...    36   6.9    2
(AM465119) Vitis vinifera contig VV78X277202.14, whole genom...    40   6.9    2
(EV423283) 210823811 NGP Nasonia giraulti cDNA clone 138E7 3...    34   7.1    2
(AY863213) Smittium culisetae mitochondrion, complete genome.      38   7.3    4
(CW723895) MARC_1178346 CHORI-240-123E2 Bos taurus genomic c...    36   7.4    2
(EV416372) 210755279 NGP Nasonia giraulti cDNA clone 131E15 ...    36   7.4    2
(ES831833) UFL_246_71 Cotton fiber 0-10 day post anthesis Go...    34   7.5    2
(EJ775844) 1093000100329 Global-Ocean-Sampling_GS-30-02-01-1...    34   7.5    2
(EV419904) 210786753 NGP Nasonia giraulti cDNA clone 175O7 5...    36   7.6    2
(EV415183) 210744588 NGP Nasonia giraulti cDNA clone 130M3 5...    36   7.6    2
(EV416354) 210761035 NGP Nasonia giraulti cDNA clone 180K9 5...    36   7.7    2
(EV416797) 210761631 NGP Nasonia giraulti cDNA clone 177M15 ...    36   7.8    2
(EV423598) 211343177 NGP Nasonia giraulti cDNA clone 234K1, ...    36   7.8    2
(EV419515) 210781878 NGP Nasonia giraulti cDNA clone 159K7 5...    36   8.0    2
(AM481015) Vitis vinifera contig VV78X047372.5, whole genome...    38   8.1    3
(EJ743511) 1092962037194 Global-Ocean-Sampling_GS-30-02-01-1...    34   8.2    2
(AC153715) Bos taurus clone CH240-22F9, WORKING DRAFT SEQUEN...    32   8.4    2
(AC140462) Mus musculus BAC clone RP23-13K8 from 14, complet...    32   8.5    2
(AC221331) Bos taurus clone CH240-403M11, WORKING DRAFT SEQU...    32   8.5    2
(AC115519) Rattus norvegicus clone CH230-384A21, WORKING DRA...    32   8.6    2
(AC021742) Homo sapiens chromosome 7 clone RP11-202P1 map 7,...    30   8.7    2
(AC106716) Homo sapiens 3 BAC RP11-202P1 (Roswell Park Cance...    30   8.7    2
(AP010433) Lotus japonicus genomic DNA, chromosome 5, clone:...    30   8.9    2
(AL845362) Zebrafish DNA sequence from clone CH211-142H2 in ...    38   9.2    3
(DJ386701) Diagnosis of Diseases Associated with Metastasis.       40   9.2    2
(AX281497) Sequence 161 from Patent WO0177376.                     40   9.2    2

>(BJ354059) Dictyostelium discoideum cDNA clone:dda52k07, 3' end,
           single read.
          Length = 438

 Score =  359 bits (181), Expect(3) = e-157
 Identities = 181/181 (100%)
 Strand = Plus / Minus


Query: 195 tcaactaccatcttaaaagtttctgaagcatgtgaaagtttacaagattattgtaccaaa 254
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 245 tcaactaccatcttaaaagtttctgaagcatgtgaaagtttacaagattattgtaccaaa 186


Query: 255 acaagtgatccatttatcccaggttggtcaggtgaaaatgaatggactaaaccactcaaa 314
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 185 acaagtgatccatttatcccaggttggtcaggtgaaaatgaatggactaaaccactcaaa 126


Query: 315 ggaaatggatgttctgtgttataaatattatcaacttatacattgtacattatatagaat 374
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 125 ggaaatggatgttctgtgttataaatattatcaacttatacattgtacattatatagaat 66


Query: 375 t 375
           |
Sbjct: 65  t 65

 Score =  151 bits (76), Expect(3) = e-157
 Identities = 78/79 (98%)
 Strand = Plus / Minus


Query: 1   aaaacgcaactttgtacacataaaatcacacgctcaaaacccatataaattatttaattt 60
           |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct: 438 aaaacgcaactttgtacacataaaatcacacgntcaaaacccatataaattatttaattt 379


Query: 61  aaaccataatttttaataa 79
           |||||||||||||||||||
Sbjct: 378 aaaccataatttttaataa 360

 Score =  105 bits (53), Expect(3) = e-157
 Identities = 53/53 (100%)
 Strand = Plus / Minus


Query: 93  ctctattaattaaataattttaacaatacacacataatgtccgaatcacaatt 145
           |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 347 ctctattaattaaataattttaacaatacacacataatgtccgaatcacaatt 295

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 412,413,593
Number of extensions: 28206478
Number of successful extensions: 2431960
Number of sequences better than 10.0: 92
Length of query: 506
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 483
Effective length of database: 93,106,754,628
Effective search space: 44970562485324
Effective search space used: 44970562485324
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

Query= Contig-U14102-1 (Contig-U14102-1Q) /CSM_Contig/Contig-U14102-1Q.Seq.d
         (506 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF687895_1(EF687895|pid:none) Paracoccidioides brasiliensis Gpg1...    38   0.16
AY907677_1(AY907677|pid:none) Cryptococcus neoformans var. grubi...    35   1.3
DQ863327_1(DQ863327|pid:none) Penicillium marneffei STE20-like p...    33   3.0
DQ677630_1(DQ677630|pid:none) Aspergillus fumigatus G protein ga...    33   3.0
DQ863322_1(DQ863322|pid:none) Penicillium marneffei STE18-like p...    33   3.9
DQ677631_1(DQ677631|pid:none) Emericella nidulans G protein gamm...    33   3.9
(Q61012) RecName: Full=Guanine nucleotide-binding protein G(T) s...    33   3.9

>EF687895_1(EF687895|pid:none) Paracoccidioides brasiliensis Gpg1
           gene, complete cds.
          Length = 91

 Score = 37.7 bits (86), Expect = 0.16
 Identities = 24/73 (32%), Positives = 40/73 (54%), Gaps = 4/73 (5%)
 Frame = +3

Query: 129 MSESQLKKVLKENETLKAQLEKSTTILKVSEACESLQDYCTKTSDPFIPGWSGENEWTK- 305
           M+E +L+++ + N  LK  LE+    +KVSEA  SL +YC  T D  +P   G+ +  +
Sbjct: 20  MAELKLRRLNELNVRLKEDLERPR--VKVSEAAMSLINYCNNTRDFMVPSVWGQIDKRED 77

Query: 306 ---PLKGNGCSVL 335
              P +  GC ++
Sbjct: 78  PYAPQQSGGCCMI 90

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 531,157,770
Number of extensions: 7701635
Number of successful extensions: 18116
Number of sequences better than 10.0: 7
Number of HSP's gapped: 18115
Number of HSP's successfully gapped: 7
Length of query: 168
Length of database: 1,051,180,864
Length adjustment: 119
Effective length of query: 49
Effective length of database: 666,030,343
Effective search space: 32635486807
Effective search space used: 32635486807
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)

 psg: 0.75  gvh: 0.42  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 1.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: cytoplasmic
	 20.0 %: plasma membrane
	 20.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  8.0 %: Golgi
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for Contig-U14102-1 is cyt