>Contig-U14000-1 (Contig-U14000-1Q) /CSM_Contig/Contig-U14000-1Q.Seq.d
AAAATTGCATGTGGAAATAAATCAACTTTTTTAATAAAAAAAAAATTATA
GTGACAGTGAACTTAAATTTGGTTTATTTTATTTTTTTTTTATTTTTTTA
ATTTTTTTTTTTTTTTTTCCCAGGGNAAAAAAANNTTTTAAANTAAAAAA
NTTTNGNGNAAAAAAAAANTTT

est1=CHE819F,1,152
est2=AHA366F,2,151
est3=VHD565F,2,172
est4=CHI817F,48,150
est5=CHQ161F,48,172
est6=AHI168F,56,167

klhveinqlf**KKNYSDSELKFGLFYFFFIFLIFFFFFPRXKKXFKXKXFXXKKXF

Frame A:
kiacgnkstflikkkl**q*t*iwfilfffyffnfffffsqgkkxf*xkkxxxkkkx


Frame B:
klhveinqlf**KKNYSDSELKFGLFYFFFIFLIFFFFFPRXKKXFKXKXFXXKKXF


Frame C:
ncmwk*inffnkkkiivtvnlnlvyfifflff*ffffffpgxkxxlx*kxxxxkkx

Query= Contig-U14000-1 (Contig-U14000-1Q)
/CSM_Contig/Contig-U14000-1Q.Seq.d
         (172 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U14000-1 (Contig-U14000-1Q) /CSM_Contig/Conti...    84  5e-17
Contig-U11303-1 (Contig-U11303-1Q) /CSM_Contig/Conti...    32  0.18
Contig-U06933-1 (Contig-U06933-1Q) /CSM_Contig/Conti...    30  0.70
Contig-U05047-1 (Contig-U05047-1Q) /CSM_Contig/Conti...    30  0.70
Contig-U12581-1 (Contig-U12581-1Q) /CSM_Contig/Conti...    28  2.7
Contig-U12426-1 (Contig-U12426-1Q) /CSM_Contig/Conti...    28  2.7
Contig-U11302-1 (Contig-U11302-1Q) /CSM_Contig/Conti...    28  2.7
Contig-U11101-1 (Contig-U11101-1Q) /CSM_Contig/Conti...    28  2.7
Contig-U09368-1 (Contig-U09368-1Q) /CSM_Contig/Conti...    28  2.7
Contig-U07029-1 (Contig-U07029-1Q) /CSM_Contig/Conti...    28  2.7

>Contig-U14000-1 (Contig-U14000-1Q) /CSM_Contig/Contig-U14000-1Q.Seq.d
          Length = 172

 Score = 83.8 bits (42), Expect = 5e-17
 Identities = 62/72 (86%)
 Strand = Plus / Plus

                                                                      
Query: 1  aaaattgcatgtggaaataaatcaacttttttaatnnnnnnnnnnttatagtgacagtga 60
          |||||||||||||||||||||||||||||||||||          |||||||||||||||
Sbjct: 1  aaaattgcatgtggaaataaatcaacttttttaataaaaaaaaaattatagtgacagtga 60

                      
Query: 61 acttaaatttgg 72
          ||||||||||||
Sbjct: 61 acttaaatttgg 72


>Contig-U11303-1 (Contig-U11303-1Q) /CSM_Contig/Contig-U11303-1Q.Seq.d
          Length = 2115

 Score = 32.2 bits (16), Expect = 0.18
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 57  gtgaacttaaatttgg 72
           ||||||||||||||||
Sbjct: 608 gtgaacttaaatttgg 593


>Contig-U06933-1 (Contig-U06933-1Q) /CSM_Contig/Contig-U06933-1Q.Seq.d
          Length = 545

 Score = 30.2 bits (15), Expect = 0.70
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                          
Query: 21  atcaacttttttaat 35
           |||||||||||||||
Sbjct: 502 atcaacttttttaat 516


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 755
Number of Sequences: 6905
Number of extensions: 755
Number of successful extensions: 227
Number of sequences better than 10.0: 17
length of query: 172
length of database: 5,674,871
effective HSP length: 15
effective length of query: 157
effective length of database: 5,571,296
effective search space: 874693472
effective search space used: 874693472
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)

Query= Contig-U14000-1 (Contig-U14000-1Q) /CSM_Contig/Contig-U14000-1Q.Seq.d
         (172 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ364260) Dictyostelium discoideum cDNA clone:ddc31f06, 5' ...    70   1e-18  2
(AC116330) Dictyostelium discoideum chromosome 2 map 3191214...    68   3e-18  2
(BJ330433) Dictyostelium discoideum cDNA clone:dda31d17, 5' ...    68   4e-18  2
(BJ420178) Dictyostelium discoideum cDNA clone:ddv38a17, 5' ...    68   2e-16  2
(BJ370572) Dictyostelium discoideum cDNA clone:ddc54i15, 5' ...    50   0.024  1
(BJ366446) Dictyostelium discoideum cDNA clone:ddc39b06, 5' ...    50   0.024  1
(AC229768) Oryza minuta clone OM__Ba0141P22, *** SEQUENCING ...    42   5.8    1
(AC226090) Oryza officinalis clone OO__Ba0092G19, *** SEQUEN...    42   5.8    1
(AC217920) Oryza officinalis clone OO__Ba0115A22, *** SEQUEN...    42   5.8    1
(DU589348) OO__Ba0082I05.f OO__Ba Oryza officinalis genomic ...    42   5.8    1
(EY353444) CAWZ2112.rev CAWZ Helobdella robusta Primary Late...    36   9.0    2
(EY381617) CAXA3569.rev CAXA Helobdella robusta Subtracted L...    36   9.3    2
(EY381212) CAXA3327.rev CAXA Helobdella robusta Subtracted L...    36   9.8    2

>(BJ364260) Dictyostelium discoideum cDNA clone:ddc31f06, 5' end,
          single read.
          Length = 152

 Score = 69.9 bits (35), Expect(2) = 1e-18
 Identities = 35/35 (100%)
 Strand = Plus / Plus


Query: 1  aaaattgcatgtggaaataaatcaacttttttaat 35
          |||||||||||||||||||||||||||||||||||
Sbjct: 1  aaaattgcatgtggaaataaatcaacttttttaat 35

 Score = 54.0 bits (27), Expect(2) = 1e-18
 Identities = 27/27 (100%)
 Strand = Plus / Plus


Query: 46 ttatagtgacagtgaacttaaatttgg 72
          |||||||||||||||||||||||||||
Sbjct: 46 ttatagtgacagtgaacttaaatttgg 72

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 82,373,171
Number of extensions: 4326321
Number of successful extensions: 1061256
Number of sequences better than 10.0: 14
Length of query: 172
Length of database: 95,242,211,685
Length adjustment: 22
Effective length of query: 150
Effective length of database: 93,199,600,587
Effective search space: 13979940088050
Effective search space used: 13979940088050
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

Query= Contig-U14000-1 (Contig-U14000-1Q) /CSM_Contig/Contig-U14000-1Q.Seq.d
         (172 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 96,455,419
Number of extensions: 546014
Number of successful extensions: 473
Number of sequences better than 10.0: 0
Number of HSP's gapped: 473
Number of HSP's successfully gapped: 0
Length of query: 57
Length of database: 1,051,180,864
Length adjustment: 30
Effective length of query: 27
Effective length of database: 954,084,094
Effective search space: 25760270538
Effective search space used: 25760270538
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 26 (14.6 bits)

 psg: 1.05  gvh: 0.51  alm: 0.18  top: 0.87  tms: 0.07  mit: 0.23  mip: 0.00
 nuc: 0.18  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.14  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for Contig-U14000-1 is cyt