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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U13721-1
Contig ID Contig-U13721-1
Contig update 2002.12.18
Contig sequence 
>Contig-U13721-1 (Contig-U13721-1Q) /CSM_Contig/Contig-U13721-1Q.Seq.d
ATCAATTTTAGTATTTATATCATATATTTGTGCAAGTTTTGCAGCAAATA
ACAGGATTTATTTATATTCAATCACAGCTTTGGCAGTTGGATTAGGTTTA
ACTATTCCATCATTTTTTGCAGCAACAGGTGCAGTTGTTTTAGGTACTGG
TAGAGATAAATCAACTTGGGATGTAGAATTATTTAAATCTGATCAAGTTT
TAATGGGATGGATGTGGCCAAAAGGTCAAATGGCAATTTTTGTAGATGAA
AGTACAATTATTGGACCAGATTCATTTATTGGTAAATTATCAACAGAAAT
TCTTCAATTATCATATATCAGTTATTATATTTGGGGTTATTTTATGGAGA
TTTATATTTTATATAATTTATGGAGATGTCATTTATCAAAGGACCCACAA
CAACAAAAGATGATGCCAATCTGGGATCAAAGATTAAAAATGTTTATTTG
CTCATGGATTTCAACTTACTTTATAGTGTTTTCTATTAATTTAATATTCC
CAGCTGTTAGTCCAAGAGTTTATATTGGTAAAACTTTAAATCTCTATAAC
AATACCTTGGAGGGTTTTGGTTTCGCTGGTTTTGTACGTAGTAGAATTGA
TAATGCAGCAAAAGGTTCATTTGGTTCATTCCCTTCTGGTCATATAGCAC
AACTTCATTTGCAATTGGTTTATCAAGTTATAAGATTTTACCCGCTTATG
GTTTCGTTTCAACAATTGCTGCAATCTTAATCGCAATTGCTACAATGTAT
TTACGTTATCATTACTTTGTCGATTTCTTAGCTGCTTTACCTGTGACCAT
CTTTTGTTTATTATATGGTGGTTTCTATACACCTTCCGACTTTAAAAATG
TTTTTGTAAATTGTTTTTACTCGATAAAATCAATCTTTCAAAATATTTTA
TCAAAATTTAATAATAAATAATAAATCTATTTAAAAAATAAAAAAAAAAA
AAAAAAA
Gap no gap
Contig length 957
Chromosome number (1..6, M) 1
Chromosome length 4919822
Start point 1904945
End point 1905902
Strand (PLUS/MINUS) PLUS
Number of clones 2
Number of EST 2
Link to clone list U13721
List of clone(s) 

est1=SSG254E,1,958
est2=VHK531Z,264,725
Translated Amino Acid sequence 
SILVFISYICASFAANNRIYLYSITALAVGLGLTIPSFFAATGAVVLGTGRDKSTWDVEL
FKSDQVLMGWMWPKGQMAIFVDESTIIGPDSFIGKLSTEILQLSYISYYIWGYFMEIYIL
YNLWRCHLSKDPQQQKMMPIWDQRLKMFICSWISTYFIVFSINLIFPAVSPRVYIGKTLN
LYNNTLEGFGFAGFVRSRIDNAAKGSFGSFPSGHIAQLHLQLVYQVIRFYPLMVSFQQLL
QS*sqllqciyviitlsis*llyl*psfvyymvvsihlptlkmfl*ivftr*nqsfkify
qnliinnksi*kikkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
infsiyiiylckfcsk*qdlfifnhsfgswirfnysiifcsnrcscfryw*r*inlgcri
i*i*ssfngmdvakrsngnfcr*kynywtrfiyw*iinrnssiiiyqllylglfygdlyf
i*fmemsfikgptttkddanlgskiknvyllmdfnllysvfy*fnipsc*skslyw*nfk
sl*qylggfwfrwfct**n**cskrfiwfipfwsysttsfaiglssykilpaygfvstia
ailiaiatmylryhyfvdflaalpvtifcllyggfytpsdfknvfvncfysiksifqnil
skfnnk**iylknkkkkkk


Frame B:
SILVFISYICASFAANNRIYLYSITALAVGLGLTIPSFFAATGAVVLGTGRDKSTWDVEL
FKSDQVLMGWMWPKGQMAIFVDESTIIGPDSFIGKLSTEILQLSYISYYIWGYFMEIYIL
YNLWRCHLSKDPQQQKMMPIWDQRLKMFICSWISTYFIVFSINLIFPAVSPRVYIGKTLN
LYNNTLEGFGFAGFVRSRIDNAAKGSFGSFPSGHIAQLHLQLVYQVIRFYPLMVSFQQLL
QS*sqllqciyviitlsis*llyl*psfvyymvvsihlptlkmfl*ivftr*nqsfkify
qnliinnksi*kikkkkk


Frame C:
qf*ylyhifvqvlqqitgfiyiqsqlwqld*v*lfhhflqqqvqlf*vlveinqlgm*ny
lnlikf*wdgcgqkvkwqfl*mkvqlldqihllvnyqqkffnyhisviifgvilwrfify
iiygdviyqrthnnkr*cqsgikd*kclfahgfqltl*cflli*ysqllvqefilvkl*i
sitipwrvlvslvlyvvelimqqkvhlvhsllvi*hnficnwfikl*dftrlwfrfnncc
nlnrncynvftlsllcrflscftcdhllfiiwwflytfrl*kcfcklflldkinlskyfi
ki***iinlfkk*kkkkk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U13721-1 (Contig-U13721-1Q)
/CSM_Contig/Contig-U13721-1Q.Seq.d
         (957 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U13721-1 (Contig-U13721-1Q) /CSM_Contig/Conti...   993  0.0
Contig-U11360-1 (Contig-U11360-1Q) /CSM_Contig/Conti...    38  0.017
Contig-U13961-1 (Contig-U13961-1Q) /CSM_Contig/Conti...    36  0.067
Contig-U13547-1 (Contig-U13547-1Q) /CSM_Contig/Conti...    36  0.067
Contig-U06795-1 (Contig-U06795-1Q) /CSM_Contig/Conti...    36  0.067
Contig-U00581-1 (Contig-U00581-1Q) /CSM_Contig/Conti...    36  0.067
Contig-U14726-1 (Contig-U14726-1Q) /CSM_Contig/Conti...    34  0.27
Contig-U13390-1 (Contig-U13390-1Q) /CSM_Contig/Conti...    34  0.27
Contig-U13280-1 (Contig-U13280-1Q) /CSM_Contig/Conti...    34  0.27
Contig-U12338-1 (Contig-U12338-1Q) /CSM_Contig/Conti...    34  0.27

>Contig-U13721-1 (Contig-U13721-1Q) /CSM_Contig/Contig-U13721-1Q.Seq.d
          Length = 957

 Score =  993 bits (501), Expect = 0.0
 Identities = 501/501 (100%)
 Strand = Plus / Plus

                                                                       
Query: 372 ggagatgtcatttatcaaaggacccacaacaacaaaagatgatgccaatctgggatcaaa 431
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 372 ggagatgtcatttatcaaaggacccacaacaacaaaagatgatgccaatctgggatcaaa 431

                                                                       
Query: 432 gattaaaaatgtttatttgctcatggatttcaacttactttatagtgttttctattaatt 491
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 432 gattaaaaatgtttatttgctcatggatttcaacttactttatagtgttttctattaatt 491

                                                                       
Query: 492 taatattcccagctgttagtccaagagtttatattggtaaaactttaaatctctataaca 551
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 492 taatattcccagctgttagtccaagagtttatattggtaaaactttaaatctctataaca 551

                                                                       
Query: 552 ataccttggagggttttggtttcgctggttttgtacgtagtagaattgataatgcagcaa 611
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 552 ataccttggagggttttggtttcgctggttttgtacgtagtagaattgataatgcagcaa 611

                                                                       
Query: 612 aaggttcatttggttcattcccttctggtcatatagcacaacttcatttgcaattggttt 671
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 612 aaggttcatttggttcattcccttctggtcatatagcacaacttcatttgcaattggttt 671

                                                                       
Query: 672 atcaagttataagattttacccgcttatggtttcgtttcaacaattgctgcaatcttaat 731
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 672 atcaagttataagattttacccgcttatggtttcgtttcaacaattgctgcaatcttaat 731

                                                                       
Query: 732 cgcaattgctacaatgtatttacgttatcattactttgtcgatttcttagctgctttacc 791
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 732 cgcaattgctacaatgtatttacgttatcattactttgtcgatttcttagctgctttacc 791

                                                                       
Query: 792 tgtgaccatcttttgtttattatatggtggtttctatacaccttccgactttaaaaatgt 851
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 792 tgtgaccatcttttgtttattatatggtggtttctatacaccttccgactttaaaaatgt 851

                                
Query: 852 ttttgtaaattgtttttactc 872
           |||||||||||||||||||||
Sbjct: 852 ttttgtaaattgtttttactc 872


 Score =  634 bits (320), Expect = 0.0
 Identities = 320/320 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   atcaattttagtatttatatcatatatttgtgcaagttttgcagcaaataacaggattta 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   atcaattttagtatttatatcatatatttgtgcaagttttgcagcaaataacaggattta 60

                                                                       
Query: 61  tttatattcaatcacagctttggcagttggattaggtttaactattccatcattttttgc 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tttatattcaatcacagctttggcagttggattaggtttaactattccatcattttttgc 120

                                                                       
Query: 121 agcaacaggtgcagttgttttaggtactggtagagataaatcaacttgggatgtagaatt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 agcaacaggtgcagttgttttaggtactggtagagataaatcaacttgggatgtagaatt 180

                                                                       
Query: 181 atttaaatctgatcaagttttaatgggatggatgtggccaaaaggtcaaatggcaatttt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atttaaatctgatcaagttttaatgggatggatgtggccaaaaggtcaaatggcaatttt 240

                                                                       
Query: 241 tgtagatgaaagtacaattattggaccagattcatttattggtaaattatcaacagaaat 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tgtagatgaaagtacaattattggaccagattcatttattggtaaattatcaacagaaat 300

                               
Query: 301 tcttcaattatcatatatca 320
           ||||||||||||||||||||
Sbjct: 301 tcttcaattatcatatatca 320


>Contig-U11360-1 (Contig-U11360-1Q) /CSM_Contig/Contig-U11360-1Q.Seq.d
          Length = 3470

 Score = 38.2 bits (19), Expect = 0.017
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                               
Query: 850  gtttttgtaaattgttttt 868
            |||||||||||||||||||
Sbjct: 2759 gtttttgtaaattgttttt 2777


>Contig-U13961-1 (Contig-U13961-1Q) /CSM_Contig/Contig-U13961-1Q.Seq.d
          Length = 380

 Score = 36.2 bits (18), Expect = 0.067
 Identities = 21/22 (95%)
 Strand = Plus / Minus

                                 
Query: 6   ttttagtatttatatcatatat 27
           ||||||||||||||| ||||||
Sbjct: 150 ttttagtatttatattatatat 129


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 12,416
Number of Sequences: 6905
Number of extensions: 12416
Number of successful extensions: 1224
Number of sequences better than 10.0: 106
length of query: 957
length of database: 5,674,871
effective HSP length: 16
effective length of query: 941
effective length of database: 5,564,391
effective search space: 5236091931
effective search space used: 5236091931
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.12.13
Homology vs DNA 
Query= Contig-U13721-1 (Contig-U13721-1Q) /CSM_Contig/Contig-U13721-1Q.Seq.d
         (957 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443267) Dictyostelium discoideum cDNA clone:ddv52m07, 3' ...   521   0.0    3
(C89846) Dictyostelium discoideum slug cDNA, clone SSG254.        444   0.0    2
(AU073146) Dictyostelium discoideum slug cDNA, clone SSG254.      476   e-130  1
(CK398375) AGENCOURT_17357154 NIH_ZGC_4 Danio rerio cDNA clo...    38   0.52   2
(CS468880) Sequence 71 from Patent EP1748080.                      42   0.53   7
(CS468879) Sequence 70 from Patent EP1748080.                      42   0.53   7
(CS419357) Sequence 71 from Patent WO2006094836.                   42   0.53   7
(CS419356) Sequence 70 from Patent WO2006094836.                   42   0.53   7
(AJ889271) Drosophila melanogster X chromosome fragment, NV2...    48   0.61   1
(CU582816) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.61   1
(AC161793) Mus musculus chromosome 7, clone RP23-80J7, compl...    38   1.2    6
(CU062456) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    34   1.3    2
(CU856519) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    34   1.3    2
(CU062667) Zebrafish DNA sequence from clone DKEYP-110C12 in...    34   1.3    2
(ED310873) AUAC-aau65d05.g1 Ascaris suum whole genome shotgu...    36   1.7    2
(AP006198) Homo sapiens genomic DNA, chromosome 6, clone:RP1...    46   2.4    1
(AP002530) Homo sapiens genomic DNA, chromosome 6q21, anti-o...    46   2.4    1
(AL355532) Human DNA sequence from clone RP11-487F5 on chrom...    46   2.4    1
(AB042030) Homo sapiens genomic DNA, chromosome 6q21, BAC cl...    46   2.4    1
(FC222062) CAGF6755.rev CAGF Nematostella vectensis Nemve Ea...    46   2.4    1
(CP000107) Ehrlichia canis str. Jake, complete genome.             46   2.4    1
(BA000011) Thermoplasma volcanium GSS1 DNA, complete genome.       32   4.0    2
(AC179752) Strongylocentrotus purpuratus clone R3-1018C04, W...    32   4.6    2
(CU695251) Brassica rapa subsp. pekinensis clone KBrB001E05,...    36   7.8    3
(BX005258) Zebrafish DNA sequence from clone DKEY-77I24 in l...    44   9.6    1
(AP005858) Mus musculus genomic DNA, chromosome 2 clone:B478...    44   9.6    1
(AP003923) Mus musculus genomic DNA, chromosome 2, clone:B47...    44   9.6    1
(AC214220) Macaca mulatta  BAC CH250-259N13 (Children's Hosp...    44   9.6    1
(AC198805) Pan troglodytes BAC clone CH251-420M9 from chromo...    44   9.6    1
(AC192482) Pan troglodytes BAC clone CH251-335L15 from chrom...    44   9.6    1
(AC131949) Homo sapiens BAC clone RP11-240J11 from 4, comple...    44   9.6    1
(AC130895) Homo sapiens 12 BAC RP11-115H15 (Roswell Park Can...    44   9.6    1
(AC156727) Bos taurus clone CH240-50E11, WORKING DRAFT SEQUE...    44   9.6    1
(AC130148) Rattus norvegicus clone CH230-49B15, *** SEQUENCI...    44   9.6    1
(AC090040) Homo sapiens chromosome 12 clone RP11-115M5, *** ...    44   9.6    1
(AC068398) Homo sapiens chromosome 2 clone RP11-73N12 map 2,...    44   9.6    1
(AP002333) Homo sapiens genomic DNA, chromosome 4q22-q24, cl...    44   9.6    1
(AC216359) Macaca mulatta clone CH250-6J5, WORKING DRAFT SEQ...    44   9.6    1
(AC184456) Strongylocentrotus purpuratus clone R3-3082I9, WO...    44   9.6    1
(AC019249) Homo sapiens clone RP11-115M5, WORKING DRAFT SEQU...    44   9.6    1
(ET102670) QM0AAB21AF04RM1 CCER1 Citrus clementina genomic c...    44   9.6    1
(ET078397) QM0AAB6CC11FM2 CCER1 Citrus clementina genomic cl...    44   9.6    1
(EK064905) 1092960110396 Global-Ocean-Sampling_GS-31-01-01-1...    44   9.6    1
(DU687770) Bst020306 lambda03 Boechera stricta genomic clone...    44   9.6    1
(DH652873) Rattus norvegicus DNA, BAC clone: RNB1-196H21, 3'...    44   9.6    1
(DH417346) Oryzias latipes Fosmid clone:GOLWFno827_i21, reve...    44   9.6    1
(DH236049) Oryzias latipes Fosmid clone:GOLWFno506_k16, forw...    44   9.6    1
(CW916376) RPCI42_160E22.TJ RPCI-42 Bos taurus genomic clone...    44   9.6    1
(BX191604) Danio rerio genomic clone DKEY-201G9, genomic sur...    44   9.6    1
(DY525480) BAAC-PNP1304F17.g1 C.remanei EST SB146 Caenorhabd...    44   9.6    1
(FG452312) 010603KAFB006618HT (KAFB) Actinidia deliciosa bud...    44   9.6    1
(FG451476) 010602KAFB005585HT (KAFB) Actinidia deliciosa bud...    44   9.6    1
(CP001034) Natranaerobius thermophilus JW/NM-WN-LF, complete...    44   9.6    1
(AJ880319) Bacillus licheniformis partial gapN gene for NADP...    44   9.6    1
(AJ880318) Bacillus cereus partial gapN gene for NADP-depend...    44   9.6    1
(AC121090) Mus musculus chromosome 15 clone RP24-189A18 map ...    38   9.7    5

>(BJ443267) Dictyostelium discoideum cDNA clone:ddv52m07, 3' end,
           single read.
          Length = 460

 Score =  521 bits (263), Expect(3) = 0.0
 Identities = 263/263 (100%)
 Strand = Plus / Minus


Query: 387 caaaggacccacaacaacaaaagatgatgccaatctgggatcaaagattaaaaatgttta 446
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 337 caaaggacccacaacaacaaaagatgatgccaatctgggatcaaagattaaaaatgttta 278


Query: 447 tttgctcatggatttcaacttactttatagtgttttctattaatttaatattcccagctg 506
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 277 tttgctcatggatttcaacttactttatagtgttttctattaatttaatattcccagctg 218


Query: 507 ttagtccaagagtttatattggtaaaactttaaatctctataacaataccttggagggtt 566
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 217 ttagtccaagagtttatattggtaaaactttaaatctctataacaataccttggagggtt 158


Query: 567 ttggtttcgctggttttgtacgtagtagaattgataatgcagcaaaaggttcatttggtt 626
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 157 ttggtttcgctggttttgtacgtagtagaattgataatgcagcaaaaggttcatttggtt 98


Query: 627 cattcccttctggtcatatagca 649
           |||||||||||||||||||||||
Sbjct: 97  cattcccttctggtcatatagca 75

 Score =  151 bits (76), Expect(3) = 0.0
 Identities = 76/76 (100%)
 Strand = Plus / Minus


Query: 650 caacttcatttgcaattggtttatcaagttataagattttacccgcttatggtttcgttt 709
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 76  caacttcatttgcaattggtttatcaagttataagattttacccgcttatggtttcgttt 17


Query: 710 caacaattgctgcaat 725
           ||||||||||||||||
Sbjct: 16  caacaattgctgcaat 1

 Score = 85.7 bits (43), Expect(3) = 0.0
 Identities = 43/43 (100%)
 Strand = Plus / Minus


Query: 264 gaccagattcatttattggtaaattatcaacagaaattcttca 306
           |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 460 gaccagattcatttattggtaaattatcaacagaaattcttca 418

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 997,071,984
Number of extensions: 60271274
Number of successful extensions: 4942367
Number of sequences better than 10.0: 58
Length of query: 957
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 933
Effective length of database: 97,308,875,965
Effective search space: 90789181275345
Effective search space used: 90789181275345
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 8
Homology vs Protein 
Query= Contig-U13721-1 (Contig-U13721-1Q) /CSM_Contig/Contig-U13721-1Q.Seq.d
         (957 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001176_1853(CP001176|pid:none) Bacillus cereus B4264, complete...    39   0.26
CP000001_1779(CP000001|pid:none) Bacillus cereus E33L, complete ...    39   0.26
AE017194_2053(AE017194|pid:none) Bacillus cereus ATCC 10987, com...    39   0.26
CP000133_3352(CP000133|pid:none) Rhizobium etli CFN 42, complete...    38   0.45
CP000227_1869(CP000227|pid:none) Bacillus cereus Q1, complete ge...    38   0.45
CP000853_2167(CP000853|pid:none) Alkaliphilus oremlandii OhILAs,...    37   1.0
CP000271_2150(CP000271|pid:none) Burkholderia xenovorans LB400 c...    35   3.8
CP000806_1021(CP000806|pid:none) Cyanothece sp. ATCC 51142 circu...    35   3.8
CP000473_5381(CP000473|pid:none) Solibacter usitatus Ellin6076, ...    28   3.9
AF003384_4(AF003384|pid:none) Caenorhabditis elegans cosmid K07B...    35   5.0
(Q10142) RecName: Full=Aureobasidin A resistance protein homolog...    34   6.5
EU851663_1(EU851663|pid:none) Hoplitis leucomelana long-waveleng...    34   8.5

>CP001176_1853(CP001176|pid:none) Bacillus cereus B4264, complete
           genome.
          Length = 205

 Score = 38.9 bits (89), Expect = 0.26
 Identities = 23/72 (31%), Positives = 42/72 (58%), Gaps = 2/72 (2%)
 Frame = +2

Query: 287 LSTEILQLSYI--SYYIWGYFMEIYILYNLWRCHLSKDPQQQKMMPIWDQRLKMFICSWI 460
           +S  +L  S++  S YI G+F  +Y+ + +WR + S D +Q+K MP+  Q L     S +
Sbjct: 62  VSLVLLTFSWLTNSLYIVGFFFLLYMGFVIWRSNPSNDVKQEKSMPLKKQILFAASVSLL 121

Query: 461 STYFIVFSINLI 496
           + + I+ +I +I
Sbjct: 122 NPHAILDTIGVI 133

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,435,845,972
Number of extensions: 27612097
Number of successful extensions: 76878
Number of sequences better than 10.0: 12
Number of HSP's gapped: 76849
Number of HSP's successfully gapped: 13
Length of query: 319
Length of database: 1,051,180,864
Length adjustment: 128
Effective length of query: 191
Effective length of database: 636,901,312
Effective search space: 121648150592
Effective search space used: 121648150592
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.94  gvh: 0.69  alm: 0.31  top: 0.53  tms: 0.13  mit: 0.32  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U13721-1 is mit
VS (DIR, S) 0
VH (FL, L) 1
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0