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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U13574-1
Contig ID Contig-U13574-1
Contig update 2002.12.18
Contig sequence 
>Contig-U13574-1 (Contig-U13574-1Q) /CSM_Contig/Contig-U13574-1Q.Seq.d
NNNNNNNNNNCAAGAAAAAGAAAAAGAAAAAGAAAGATTAGAAAAAGAAA
AACAAAAATCAAATGATTTAGGTTTAGATTTTGGTGGTGGTGGCGGTGGT
AGTGGTTTCTTTAATAAACCGTCATATGATTTGGGTTTAGATTTTGGTGG
TGGAGGCGGTAGTAGTAGTGGTTTCTTTAGTAAACCTTCAAATGATTTAG
GTTTAGATTTTGGTAGTGCACCATCAAAACCAACTTCATCATCTTATATG
GGACTAGATTTCAATACACCAACTCAATCAAATTCAAATGATTTAGGATT
AGATTTTGGTAGTACACCATCAAAACCATCGACTTCTTCATCTTATATGG
GACTAGATTTCAATACACCAACTCAATCATCATCATCAAATTCAAATAAT
TATGGTTTAGACTTTGGTAGTACACCATCAAAACCAGAAAATTCATCATC
AAATTATATGGGATTAGATTTCAATACACCAAGTCAATCATCATCTTCAA
ATAATTATGGTTTAGACTTTGGTGCACCATCATCAACCACCACCAATAAT
AATAATAATAATAGTTGGGATTTCTTAGNATAGTAATAAACCAAAAGAAG
AATCAATTAAAGNAGNATNNAAGAAGAAA
Gap no gap
Contig length 629
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 843914
End point 843294
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U13574
List of clone(s) 

est1=VHC386Z,1,620
Translated Amino Acid sequence 
XXXQEKEKEKERLEKEKQKSNDLGLDFGGGGGGSGFFNKPSYDLGLDFGGGGGSSSGFFS
KPSNDLGLDFGSAPSKPTSSSYMGLDFNTPTQSNSNDLGLDFGSTPSKPSTSSSYMGLDF
NTPTQSSSSNSNNYGLDFGSTPSKPENSSSNYMGLDFNTPSQSSSSNNYGLDFGAPSSTT
TNNNNNNSWDFLX***tkrrin*xxxxee
Translated Amino Acid sequence (All Frames) 
Frame A:
xxxxrkrkrkrkirkrktkik*frfrfwwwwrw*wfl**tvi*fgfrfwwwrr***wfl*
*tfk*frfrfw*ctiktnfiilygtrfqytnsikfk*frirfw*ytiktidffilygtrf
qytnsiiiikfk*lwfrlw*ytiktrkfiiklygirfqytksiiifk*lwfrlwctiinh
hq******lgflxivinqkknqlkxxxrr


Frame B:
XXXQEKEKEKERLEKEKQKSNDLGLDFGGGGGGSGFFNKPSYDLGLDFGGGGGSSSGFFS
KPSNDLGLDFGSAPSKPTSSSYMGLDFNTPTQSNSNDLGLDFGSTPSKPSTSSSYMGLDF
NTPTQSSSSNSNNYGLDFGSTPSKPENSSSNYMGLDFNTPSQSSSSNNYGLDFGAPSSTT
TNNNNNNSWDFLX***tkrrin*xxxxee


Frame C:
xxxkkkkkkkkd*kkknknqmi*v*ilvvvavvvvslinrhmiwv*ilvveavvvvvslv
nlqmi*v*ilvvhhqnqlhhliwd*isihqlnqiqmi*d*ilvvhhqnhrllhliwd*is
ihqlnhhhqiqiimv*tlvvhhqnqkihhqiiwd*isihqvnhhlqiimv*tlvhhhqpp
piiiiiivgis*xsnkpkeesikxxxkkk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U13574-1 (Contig-U13574-1Q)
/CSM_Contig/Contig-U13574-1Q.Seq.d
         (629 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U13574-1 (Contig-U13574-1Q) /CSM_Contig/Conti...   963  0.0
Contig-U12737-1 (Contig-U12737-1Q) /CSM_Contig/Conti...   502  e-142
Contig-U11253-1 (Contig-U11253-1Q) /CSM_Contig/Conti...    44  2e-04
Contig-U11144-1 (Contig-U11144-1Q) /CSM_Contig/Conti...    44  2e-04
Contig-U09280-1 (Contig-U09280-1Q) /CSM_Contig/Conti...    44  2e-04
Contig-U04046-1 (Contig-U04046-1Q) /CSM_Contig/Conti...    44  2e-04
Contig-U13938-1 (Contig-U13938-1Q) /CSM_Contig/Conti...    42  7e-04
Contig-U11698-1 (Contig-U11698-1Q) /CSM_Contig/Conti...    42  7e-04
Contig-U09049-1 (Contig-U09049-1Q) /CSM_Contig/Conti...    42  7e-04
Contig-U14413-1 (Contig-U14413-1Q) /CSM_Contig/Conti...    40  0.003

>Contig-U13574-1 (Contig-U13574-1Q) /CSM_Contig/Contig-U13574-1Q.Seq.d
          Length = 629

 Score =  963 bits (486), Expect = 0.0
 Identities = 486/486 (100%)
 Strand = Plus / Plus

                                                                       
Query: 59  tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 118
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 59  tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 118

                                                                       
Query: 119 ccgtcatatgatttgggtttagattttggtggtggaggcggtagtagtagtggtttcttt 178
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 119 ccgtcatatgatttgggtttagattttggtggtggaggcggtagtagtagtggtttcttt 178

                                                                       
Query: 179 agtaaaccttcaaatgatttaggtttagattttggtagtgcaccatcaaaaccaacttca 238
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 179 agtaaaccttcaaatgatttaggtttagattttggtagtgcaccatcaaaaccaacttca 238

                                                                       
Query: 239 tcatcttatatgggactagatttcaatacaccaactcaatcaaattcaaatgatttagga 298
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 239 tcatcttatatgggactagatttcaatacaccaactcaatcaaattcaaatgatttagga 298

                                                                       
Query: 299 ttagattttggtagtacaccatcaaaaccatcgacttcttcatcttatatgggactagat 358
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 299 ttagattttggtagtacaccatcaaaaccatcgacttcttcatcttatatgggactagat 358

                                                                       
Query: 359 ttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtttagactttggt 418
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 359 ttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtttagactttggt 418

                                                                       
Query: 419 agtacaccatcaaaaccagaaaattcatcatcaaattatatgggattagatttcaataca 478
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 419 agtacaccatcaaaaccagaaaattcatcatcaaattatatgggattagatttcaataca 478

                                                                       
Query: 479 ccaagtcaatcatcatcttcaaataattatggtttagactttggtgcaccatcatcaacc 538
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 479 ccaagtcaatcatcatcttcaaataattatggtttagactttggtgcaccatcatcaacc 538

                 
Query: 539 accacc 544
           ||||||
Sbjct: 539 accacc 544


 Score =  101 bits (51), Expect = 9e-22
 Identities = 66/66 (100%)
 Strand = Plus / Plus

                                                                       
Query: 564 gttgggatttcttagnatagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 623
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 564 gttgggatttcttagnatagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 623

                 
Query: 624 aagaaa 629
           ||||||
Sbjct: 624 aagaaa 629


>Contig-U12737-1 (Contig-U12737-1Q) /CSM_Contig/Contig-U12737-1Q.Seq.d
          Length = 1283

 Score =  502 bits (253), Expect = e-142
 Identities = 256/257 (99%)
 Strand = Plus / Plus

                                                                        
Query: 288  atgatttaggattagattttggtagtacaccatcaaaaccatcgacttcttcatcttata 347
            ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 943  atgatttgggattagattttggtagtacaccatcaaaaccatcgacttcttcatcttata 1002

                                                                        
Query: 348  tgggactagatttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtt 407
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1003 tgggactagatttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtt 1062

                                                                        
Query: 408  tagactttggtagtacaccatcaaaaccagaaaattcatcatcaaattatatgggattag 467
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1063 tagactttggtagtacaccatcaaaaccagaaaattcatcatcaaattatatgggattag 1122

                                                                        
Query: 468  atttcaatacaccaagtcaatcatcatcttcaaataattatggtttagactttggtgcac 527
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1123 atttcaatacaccaagtcaatcatcatcttcaaataattatggtttagactttggtgcac 1182

                             
Query: 528  catcatcaaccaccacc 544
            |||||||||||||||||
Sbjct: 1183 catcatcaaccaccacc 1199


 Score =  244 bits (123), Expect = 1e-64
 Identities = 262/308 (85%), Gaps = 24/308 (7%)
 Strand = Plus / Plus

                                                                        
Query: 126  atgatttgggtttagattttggtggtggaggcggtagtagtagtggtttctttagtaaac 185
            ||||||| |||||||||||||||||||| |||||| |||||   |||||||||| |||||
Sbjct: 880  atgatttaggtttagattttggtggtggtggcggtggtagt---ggtttctttaataaac 936

                                                                        
Query: 186  cttcaaatgatttaggtttagattttggtagtgcaccatcaaaacca---acttcatcat 242
            | ||| ||||||| || ||||||||||||||| ||||||||||||||   ||||| ||||
Sbjct: 937  cgtcatatgatttgggattagattttggtagtacaccatcaaaaccatcgacttcttcat 996

                                                                        
Query: 243  cttatatgggactagatttcaatacaccaactca---------atcaaattcaaatgatt 293
            ||||||||||||||||||||||||||||||||||         ||||||||||||| |||
Sbjct: 997  cttatatgggactagatttcaatacaccaactcaatcatcatcatcaaattcaaataatt 1056

                                                                        
Query: 294  taggattagattttggtagtacaccatcaaaaccatcgacttcttcatc---ttatatgg 350
              || ||||| ||||||||||||||||||||||||   | ||| |||||   ||||||||
Sbjct: 1057 atggtttagactttggtagtacaccatcaaaaccagaaaattcatcatcaaattatatgg 1116

                                                                        
Query: 351  gactagatttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtttag 410
            || |||||||||||||||||| ||||||||||||      ||||||||||||||||||||
Sbjct: 1117 gattagatttcaatacaccaagtcaatcatcatc------ttcaaataattatggtttag 1170

                    
Query: 411  actttggt 418
            ||||||||
Sbjct: 1171 actttggt 1178


 Score =  170 bits (86), Expect = 1e-42
 Identities = 89/90 (98%)
 Strand = Plus / Plus

                                                                       
Query: 59  tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 118
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 876 tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 935

                                         
Query: 119 ccgtcatatgatttgggtttagattttggt 148
           ||||||||||||||||| ||||||||||||
Sbjct: 936 ccgtcatatgatttgggattagattttggt 965


 Score = 91.7 bits (46), Expect = 9e-19
 Identities = 65/66 (98%), Gaps = 1/66 (1%)
 Strand = Plus / Plus

                                                                        
Query: 564  gttgggatttcttagnatagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 623
            ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1219 gttgggatttcttag-atagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 1277

                  
Query: 624  aagaaa 629
            ||||||
Sbjct: 1278 aagaaa 1283


 Score = 54.0 bits (27), Expect = 2e-07
 Identities = 27/27 (100%)
 Strand = Plus / Plus

                                      
Query: 188 tcaaatgatttaggtttagattttggt 214
           |||||||||||||||||||||||||||
Sbjct: 876 tcaaatgatttaggtttagattttggt 902


 Score = 46.1 bits (23), Expect = 5e-05
 Identities = 26/27 (96%)
 Strand = Plus / Plus

                                      
Query: 284 tcaaatgatttaggattagattttggt 310
           |||||||||||||| ||||||||||||
Sbjct: 876 tcaaatgatttaggtttagattttggt 902


 Score = 30.2 bits (15), Expect = 2.7
 Identities = 18/19 (94%)
 Strand = Plus / Plus

                              
Query: 82  tggtggtggtggcggtggt 100
           |||||||||||| ||||||
Sbjct: 389 tggtggtggtggtggtggt 407


>Contig-U11253-1 (Contig-U11253-1Q) /CSM_Contig/Contig-U11253-1Q.Seq.d
          Length = 4298

 Score = 44.1 bits (22), Expect = 2e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                  
Query: 376  atcatcatcatcaaattcaaat 397
            ||||||||||||||||||||||
Sbjct: 1273 atcatcatcatcaaattcaaat 1294


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 14,760
Number of Sequences: 6905
Number of extensions: 14760
Number of successful extensions: 3051
Number of sequences better than 10.0: 362
length of query: 629
length of database: 5,674,871
effective HSP length: 16
effective length of query: 613
effective length of database: 5,564,391
effective search space: 3410971683
effective search space used: 3410971683
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.12.11
Homology vs DNA 
Query= Contig-U13574-1 (Contig-U13574-1Q) /CSM_Contig/Contig-U13574-1Q.Seq.d
         (629 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ437955) Dictyostelium discoideum cDNA clone:ddv35l21, 3' ...   963   0.0    2
(BJ357653) Dictyostelium discoideum cDNA clone:dda64c11, 3' ...   502   e-164  4
(AM494940) Leishmania braziliensis chromosome 3.                   44   6e-06  2
(DH477779) Monosiga ovata DNA, fosmid clone: MOF-028J10, gen...    42   7e-06  2
(AC177533) Strongylocentrotus purpuratus clone R3-1027A20, W...    46   2e-04  2
(AC147108) Mus musculus BAC clone RP24-70D19 from 13, comple...    42   3e-04  2
(AC123034) Mus musculus BAC clone RP24-484E17 from chromosom...    42   3e-04  2
(AC174884) Strongylocentrotus purpuratus clone R3-1040C20, W...    42   3e-04  2
(BJ428394) Dictyostelium discoideum cDNA clone:ddv11d20, 3' ...    44   4e-04  2
(BJ431712) Dictyostelium discoideum cDNA clone:ddv15p06, 3' ...    44   7e-04  2
(BJ341772) Dictyostelium discoideum cDNA clone:dda8i01, 3' e...    44   8e-04  2
(BJ340962) Dictyostelium discoideum cDNA clone:dda4g13, 3' e...    44   8e-04  2
(BJ341826) Dictyostelium discoideum cDNA clone:dda8h08, 3' e...    44   8e-04  2
(BJ436302) Dictyostelium discoideum cDNA clone:ddv30a22, 3' ...    44   8e-04  2
(BJ372342) Dictyostelium discoideum cDNA clone:ddc12p01, 3' ...    44   8e-04  2
(AC091342) Rattus norvegicus clone CH230-1C20, WORKING DRAFT...    56   0.001  4
(BJ342238) Dictyostelium discoideum cDNA clone:dda13e06, 3' ...    44   0.001  2
(AU075934) Dictyostelium discoideum slug cDNA, clone SSA155.       44   0.001  2
(AC120285) Rattus norvegicus clone CH230-268P1, *** SEQUENCI...    42   0.001  2
(AC094624) Rattus norvegicus clone CH230-5D4, WORKING DRAFT ...    40   0.001  2
(AC094639) Rattus norvegicus clone CH230-5B14, *** SEQUENCIN...    40   0.001  2
(AC114113) Rattus norvegicus clone CH230-186O3, WORKING DRAF...    42   0.001  2
(AC109835) Rattus norvegicus clone CH230-140D3, *** SEQUENCI...    42   0.001  2
(AC095785) Rattus norvegicus clone CH230-9L20, *** SEQUENCIN...    42   0.001  2
(AC095588) Rattus norvegicus clone CH230-8I16, WORKING DRAFT...    42   0.001  2
(AC112456) Rattus norvegicus clone CH230-110N23, *** SEQUENC...    42   0.001  2
(AC166824) Mus musculus chromosome 6 clone RP23-385C17, *** ...    42   0.001  2
(AC135938) Rattus norvegicus clone CH230-345C4, WORKING DRAF...    42   0.001  2
(AC027648) Mus musculus strain C57BL6/J clone RP23-190D1, WO...    42   0.001  2
(AC139185) Mus musculus BAC clone RP24-91B1 from 6, complete...    42   0.001  2
(AC122363) Mus musculus BAC clone RP23-437C1 from chromosome...    42   0.001  2
(AC118493) Rattus norvegicus clone CH230-354I10, WORKING DRA...    42   0.001  2
(AC184586) Strongylocentrotus purpuratus clone R3-3049A23, W...    40   0.001  2
(AC122425) Mus musculus BAC clone RP24-174D10 from 3, comple...    42   0.001  2
(AC177073) Strongylocentrotus purpuratus clone R3-4004K17, W...    42   0.001  2
(AC142376) Rattus norvegicus clone CH230-488J17, WORKING DRA...    42   0.001  2
(CU407308) Zebrafish DNA sequence from clone CH211-54E20 in ...    38   0.001  2
(CR388123) Zebrafish DNA sequence from clone CH211-158E10 in...    38   0.001  2
(U66524) Dictyostelium discoideum ORFveg158 mRNA, partial cds.     44   0.001  2
(EY307514) CAWX20512.rev CAWX Helobdella robusta Primary Ear...    42   0.001  2
(DV642538) Cm_mx0_95d03_SP6 Green Shore Crab Multiple Tissue...    42   0.001  2
(DV467533) Cm_mx0_87a11_SP6 Green Shore Crab Multiple Tissue...    42   0.001  2
(AC123253) Rattus norvegicus 12 BAC CH230-230N7 (Children's ...    56   0.002  1
(AC135766) Rattus norvegicus clone CH230-209P4, WORKING DRAF...    56   0.002  1
(FC800143) CBGC3451.rev CBGC Lottia gigantea 15h 18h embryos...    34   0.004  3
(FC800144) CBGC3451.fwd CBGC Lottia gigantea 15h 18h embryos...    34   0.004  3
(AC128351) Rattus norvegicus clone CH230-52D19, *** SEQUENCI...    38   0.004  2
(AC103183) Rattus norvegicus clone CH230-105P20, WORKING DRA...    38   0.004  2
(AC106592) Rattus norvegicus clone CH230-218B1, WORKING DRAF...    42   0.004  2
(AC118097) Rattus norvegicus clone CH230-185H22, *** SEQUENC...    40   0.004  2
(AC125855) Rattus norvegicus clone CH230-435F23, WORKING DRA...    42   0.004  2
(AC181333) Strongylocentrotus purpuratus clone R3-4019D07, W...    38   0.004  2
(AC181074) Strongylocentrotus purpuratus clone R3-3047N01, W...    42   0.004  2
(AP006535) Lotus japonicus genomic DNA, chromosome 2, clone:...    42   0.004  2
(EG605971) KIDNEYF098376D23 POSSUM_01-POSSUM-KIDNEY-2KB Tric...    38   0.005  2
(FK488536) 454GmaGlobSeed223168 Soybean Seeds Containing Glo...    44   0.006  2
(EC907733) #11-1 Differentially expressed cDNA-AFLP fragment...    36   0.006  2
(AC097257) Rattus norvegicus X BAC CH230-180I13 (Children's ...    54   0.006  1
(AC118328) Rattus norvegicus clone CH230-325D7, *** SEQUENCI...    54   0.006  1
(AC105563) Rattus norvegicus clone CH230-248H4, *** SEQUENCI...    54   0.006  1
(AC096366) Rattus norvegicus clone CH230-12I1, WORKING DRAFT...    42   0.016  2
(AC099658) Rattus norvegicus clone CH230-200K4, WORKING DRAF...    36   0.016  2
(AC178730) Strongylocentrotus purpuratus clone R3-13D8, WORK...    36   0.016  2
(AC129383) Rattus norvegicus clone CH230-36A10, *** SEQUENCI...    36   0.016  2
(AC128420) Rattus norvegicus clone CH230-142B17, WORKING DRA...    36   0.016  2
(AC120938) Rattus norvegicus clone CH230-24H24, *** SEQUENCI...    36   0.016  2
(AC106542) Rattus norvegicus clone CH230-91P1, WORKING DRAFT...    36   0.016  2
(AC135443) Rattus norvegicus clone CH230-262C23, WORKING DRA...    36   0.016  2
(AC134793) Mus musculus chromosome 15, clone RP23-159M8, com...    42   0.016  2
(AC128110) Rattus norvegicus clone CH230-410C11, WORKING DRA...    36   0.016  2
(AC073703) Mus musculus clone RP23-16E5, WORKING DRAFT SEQUE...    36   0.016  2
(AC118018) Mus musculus chromosome 17, clone RP23-458D20, co...    36   0.016  2
(AC064811) Homo sapiens chromosome 4 clone RP11-638N24 map 4...    38   0.016  2
(AC181162) Strongylocentrotus purpuratus clone R3-16C12, WOR...    36   0.016  2
(AC141189) Rattus norvegicus clone CH230-343I21, WORKING DRA...    36   0.016  2
(AC185159) Atelerix albiventris clone LB4-432C6, WORKING DRA...    36   0.016  2
(AC178020) Strongylocentrotus purpuratus clone R3-3055E10, W...    42   0.016  2
(AC176106) Strongylocentrotus purpuratus clone R3-24G8, WORK...    36   0.016  2
(AP010180) Lotus japonicus genomic DNA, chromosome 1, clone:...    42   0.016  2
(AP010585) Lotus japonicus genomic DNA, chromosome 1, clone:...    42   0.016  2
(AF393466) Uncultured crenarchaeote 74A4 partial genomic seq...    34   0.017  5
(FC748248) CBBI3790.fwd CBBI Lottia gigantea 26h,37h,61h Lar...    32   0.019  3
(AY764183) Aplysia californica syntaxin mRNA, complete cds.        38   0.019  2
(EY334165) CAWY6173.fwd CAWY Helobdella robusta Subtracted E...    38   0.020  2
(DC493279) Monosiga ovata cDNA, clone: MOC-062L10, 5'end.          38   0.020  2
(EY331172) CAWY3992.fwd CAWY Helobdella robusta Subtracted E...    38   0.020  2
(EY293794) CAWX11324.fwd CAWX Helobdella robusta Primary Ear...    34   0.020  3
(AI066946) SWOvAMCAQ01C02SK Onchocerca volvulus adult male c...    40   0.021  2
(DB683337) Solanum lycopersicum cDNA, clone: LEFL1016BC05, 5...    42   0.021  2
(AL031028) Drosophila melanogaster cosmid clone 56G7.              52   0.024  1
(AL031026) Drosophila melanogaster cosmid 85F11.                   52   0.024  1
(AE014298) Drosophila melanogaster chromosome X, complete se...    52   0.024  1
(AC107326) Drosophila melanogaster X BAC RP98-33A8 (Roswell ...    52   0.024  1
(AC111301) Rattus norvegicus clone CH230-225M18, *** SEQUENC...    52   0.024  1
(AC017244) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    52   0.024  1
(DN133687) 997952 MARC 4PIG Sus scrofa cDNA 5', mRNA sequence.     52   0.024  1
(DH462889) Monosiga ovata DNA, fosmid clone: MOF-002I14, gen...    42   0.030  2
(DH464450) Monosiga ovata DNA, fosmid clone: MOF-005B22, gen...    42   0.033  2
(DH477507) Monosiga ovata DNA, fosmid clone: MOF-028B05, gen...    42   0.035  2
(BQ135099) INIT1_3_C05.g1_A006 G5 trophont cDNA (INIT1) Icht...    38   0.041  2

>(BJ437955) Dictyostelium discoideum cDNA clone:ddv35l21, 3' end,
           single read.
          Length = 620

 Score =  963 bits (486), Expect(2) = 0.0
 Identities = 486/486 (100%)
 Strand = Plus / Minus


Query: 59  tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 118
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 572 tcaaatgatttaggtttagattttggtggtggtggcggtggtagtggtttctttaataaa 513


Query: 119 ccgtcatatgatttgggtttagattttggtggtggaggcggtagtagtagtggtttcttt 178
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 512 ccgtcatatgatttgggtttagattttggtggtggaggcggtagtagtagtggtttcttt 453


Query: 179 agtaaaccttcaaatgatttaggtttagattttggtagtgcaccatcaaaaccaacttca 238
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 452 agtaaaccttcaaatgatttaggtttagattttggtagtgcaccatcaaaaccaacttca 393


Query: 239 tcatcttatatgggactagatttcaatacaccaactcaatcaaattcaaatgatttagga 298
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 392 tcatcttatatgggactagatttcaatacaccaactcaatcaaattcaaatgatttagga 333


Query: 299 ttagattttggtagtacaccatcaaaaccatcgacttcttcatcttatatgggactagat 358
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 332 ttagattttggtagtacaccatcaaaaccatcgacttcttcatcttatatgggactagat 273


Query: 359 ttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtttagactttggt 418
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 272 ttcaatacaccaactcaatcatcatcatcaaattcaaataattatggtttagactttggt 213


Query: 419 agtacaccatcaaaaccagaaaattcatcatcaaattatatgggattagatttcaataca 478
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 212 agtacaccatcaaaaccagaaaattcatcatcaaattatatgggattagatttcaataca 153


Query: 479 ccaagtcaatcatcatcttcaaataattatggtttagactttggtgcaccatcatcaacc 538
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 152 ccaagtcaatcatcatcttcaaataattatggtttagactttggtgcaccatcatcaacc 93


Query: 539 accacc 544
           ||||||
Sbjct: 92  accacc 87

 Score =  101 bits (51), Expect(2) = 0.0
 Identities = 66/66 (100%)
 Strand = Plus / Minus


Query: 564 gttgggatttcttagnatagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 623
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 67  gttgggatttcttagnatagtaataaaccaaaagaagaatcaattaaagnagnatnnaag 8


Query: 624 aagaaa 629
           ||||||
Sbjct: 7   aagaaa 2

 Score = 87.7 bits (44), Expect(6) = 1e-81
 Identities = 47/48 (97%)
 Strand = Plus / Minus


Query: 233 acttcatcatcttatatgggactagatttcaatacaccaactcaatca 280
           ||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 299 acttcttcatcttatatgggactagatttcaatacaccaactcaatca 252

 Score = 87.7 bits (44), Expect(6) = 1e-81
 Identities = 47/48 (97%)
 Strand = Plus / Minus


Query: 332 acttcttcatcttatatgggactagatttcaatacaccaactcaatca 379
           ||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 398 acttcatcatcttatatgggactagatttcaatacaccaactcaatca 351

 Score = 75.8 bits (38), Expect(6) = 1e-81
 Identities = 44/46 (95%)
 Strand = Plus / Minus


Query: 187 ttcaaatgatttaggtttagattttggtagtgcaccatcaaaacca 232
           ||||||||||||||| ||||||||||||||| ||||||||||||||
Sbjct: 348 ttcaaatgatttaggattagattttggtagtacaccatcaaaacca 303

 Score = 75.8 bits (38), Expect(6) = 1e-81
 Identities = 44/46 (95%)
 Strand = Plus / Minus


Query: 283 ttcaaatgatttaggattagattttggtagtacaccatcaaaacca 328
           ||||||||||||||| ||||||||||||||| ||||||||||||||
Sbjct: 444 ttcaaatgatttaggtttagattttggtagtgcaccatcaaaacca 399

 Score = 73.8 bits (37), Expect(6) = 1e-81
 Identities = 46/49 (93%)
 Strand = Plus / Minus


Query: 343 ttatatgggactagatttcaatacaccaactcaatcatcatcatcaaat 391
           |||||||||| |||||||||||||||||| |||||||||||| ||||||
Sbjct: 177 ttatatgggattagatttcaatacaccaagtcaatcatcatcttcaaat 129

 Score = 73.8 bits (37), Expect(6) = 1e-81
 Identities = 46/49 (93%)
 Strand = Plus / Minus


Query: 454 ttatatgggattagatttcaatacaccaagtcaatcatcatcttcaaat 502
           |||||||||| |||||||||||||||||| |||||||||||| ||||||
Sbjct: 288 ttatatgggactagatttcaatacaccaactcaatcatcatcatcaaat 240

 Score = 67.9 bits (34), Expect(6) = 1e-81
 Identities = 46/50 (92%)
 Strand = Plus / Minus


Query: 99  gtagtggtttctttaataaaccgtcatatgatttgggtttagattttggt 148
           ||||||||||||||| |||||| ||| ||||||| |||||||||||||||
Sbjct: 466 gtagtggtttctttagtaaaccttcaaatgatttaggtttagattttggt 417

 Score = 67.9 bits (34), Expect(6) = 1e-81
 Identities = 46/50 (92%)
 Strand = Plus / Minus


Query: 165 gtagtggtttctttagtaaaccttcaaatgatttaggtttagattttggt 214
           ||||||||||||||| |||||| ||| ||||||| |||||||||||||||
Sbjct: 532 gtagtggtttctttaataaaccgtcatatgatttgggtttagattttggt 483

 Score = 63.9 bits (32), Expect(6) = 1e-81
 Identities = 47/52 (90%)
 Strand = Plus / Minus


Query: 277 atcaaattcaaatgatttaggattagattttggtagtacaccatcaaaacca 328
           ||||||||||||| |||  || ||||| ||||||||||||||||||||||||
Sbjct: 246 atcaaattcaaataattatggtttagactttggtagtacaccatcaaaacca 195

 Score = 63.9 bits (32), Expect(6) = 1e-81
 Identities = 47/52 (90%)
 Strand = Plus / Minus


Query: 385 atcaaattcaaataattatggtttagactttggtagtacaccatcaaaacca 436
           ||||||||||||| |||  || ||||| ||||||||||||||||||||||||
Sbjct: 354 atcaaattcaaatgatttaggattagattttggtagtacaccatcaaaacca 303

 Score = 58.0 bits (29), Expect(6) = 1e-81
 Identities = 38/41 (92%)
 Strand = Plus / Minus


Query: 63  atgatttaggtttagattttggtggtggtggcggtggtagt 103
           ||||||| |||||||||||||||||||| |||||| |||||
Sbjct: 505 atgatttgggtttagattttggtggtggaggcggtagtagt 465

 Score = 58.0 bits (29), Expect(6) = 1e-81
 Identities = 38/41 (92%)
 Strand = Plus / Minus


Query: 126 atgatttgggtttagattttggtggtggaggcggtagtagt 166
           ||||||| |||||||||||||||||||| |||||| |||||
Sbjct: 568 atgatttaggtttagattttggtggtggtggcggtggtagt 528

 Score = 58.0 bits (29), Expect(5) = 4e-36
 Identities = 35/37 (94%)
 Strand = Plus / Minus


Query: 382 atcatcaaattcaaataattatggtttagactttggt 418
           |||||||  ||||||||||||||||||||||||||||
Sbjct: 144 atcatcatcttcaaataattatggtttagactttggt 108

 Score = 58.0 bits (29), Expect(5) = 4e-36
 Identities = 35/37 (94%)
 Strand = Plus / Minus


Query: 244 ttatatgggactagatttcaatacaccaactcaatca 280
           |||||||||| |||||||||||||||||| |||||||
Sbjct: 177 ttatatgggattagatttcaatacaccaagtcaatca 141

 Score = 58.0 bits (29), Expect(5) = 4e-36
 Identities = 35/37 (94%)
 Strand = Plus / Minus


Query: 487 atcatcatcttcaaataattatggtttagactttggt 523
           |||||||  ||||||||||||||||||||||||||||
Sbjct: 249 atcatcaaattcaaataattatggtttagactttggt 213

 Score = 58.0 bits (29), Expect(5) = 4e-36
 Identities = 35/37 (94%)
 Strand = Plus / Minus


Query: 454 ttatatgggattagatttcaatacaccaagtcaatca 490
           |||||||||| |||||||||||||||||| |||||||
Sbjct: 387 ttatatgggactagatttcaatacaccaactcaatca 351

 Score = 54.0 bits (27), Expect(5) = 4e-36
 Identities = 27/27 (100%)
 Strand = Plus / Minus


Query: 59  tcaaatgatttaggtttagattttggt 85
           |||||||||||||||||||||||||||
Sbjct: 443 tcaaatgatttaggtttagattttggt 417

 Score = 54.0 bits (27), Expect(5) = 4e-36
 Identities = 27/27 (100%)
 Strand = Plus / Minus


Query: 188 tcaaatgatttaggtttagattttggt 214
           |||||||||||||||||||||||||||
Sbjct: 572 tcaaatgatttaggtttagattttggt 546

 Score = 52.0 bits (26), Expect(5) = 4e-36
 Identities = 41/46 (89%)
 Strand = Plus / Minus


Query: 187 ttcaaatgatttaggtttagattttggtagtgcaccatcaaaacca 232
           ||||||| |||  |||||||| ||||||||| ||||||||||||||
Sbjct: 240 ttcaaataattatggtttagactttggtagtacaccatcaaaacca 195

 Score = 52.0 bits (26), Expect(5) = 4e-36
 Identities = 41/46 (89%)
 Strand = Plus / Minus


Query: 391 ttcaaataattatggtttagactttggtagtacaccatcaaaacca 436
           ||||||| |||  |||||||| ||||||||| ||||||||||||||
Sbjct: 444 ttcaaatgatttaggtttagattttggtagtgcaccatcaaaacca 399

 Score = 30.2 bits (15), Expect(5) = 4e-36
 Identities = 21/23 (91%)
 Strand = Plus / Minus


Query: 126 atgatttgggtttagattttggt 148
           ||||||| || ||||||||||||
Sbjct: 343 atgatttaggattagattttggt 321

 Score = 30.2 bits (15), Expect(5) = 4e-36
 Identities = 21/23 (91%)
 Strand = Plus / Minus


Query: 288 atgatttaggattagattttggt 310
           ||||||| || ||||||||||||
Sbjct: 505 atgatttgggtttagattttggt 483

 Score = 46.1 bits (23), Expect(2) = 0.17
 Identities = 26/27 (96%)
 Strand = Plus / Minus


Query: 59  tcaaatgatttaggtttagattttggt 85
           |||||||||||||| ||||||||||||
Sbjct: 347 tcaaatgatttaggattagattttggt 321

 Score = 46.1 bits (23), Expect(2) = 0.17
 Identities = 26/27 (96%)
 Strand = Plus / Minus


Query: 284 tcaaatgatttaggattagattttggt 310
           |||||||||||||| ||||||||||||
Sbjct: 572 tcaaatgatttaggtttagattttggt 546

 Score = 26.3 bits (13), Expect(2) = 0.17
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 380 tcatcatcaaatt 392
           |||||||||||||
Sbjct: 188 tcatcatcaaatt 176

 Score = 26.3 bits (13), Expect(2) = 0.17
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 443 tcatcatcaaatt 455
           |||||||||||||
Sbjct: 251 tcatcatcaaatt 239

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 674,124,405
Number of extensions: 46053563
Number of successful extensions: 4970622
Number of sequences better than 10.0: 1227
Length of query: 629
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 606
Effective length of database: 93,106,754,628
Effective search space: 56422693304568
Effective search space used: 56422693304568
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 7
Homology vs Protein 
Query= Contig-U13574-1 (Contig-U13574-1Q) /CSM_Contig/Contig-U13574-1Q.Seq.d
         (629 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116957_76(AC116957|pid:none) Dictyostelium discoideum chromoso...    33   8.8
(Q555D2) RecName: Full=Uncharacterized protein DDB_G0274915;           33   8.8

>AC116957_76(AC116957|pid:none) Dictyostelium discoideum chromosome 2
            map 1685067-2090751 strain AX4, complete sequence.
          Length = 1806

 Score = 32.7 bits (73), Expect = 8.8
 Identities = 33/110 (30%), Positives = 45/110 (40%), Gaps = 3/110 (2%)
 Frame = +2

Query: 209  FGSAPSKPTSSSYMGLDFNTPTQSNSNDLGLDFGXXXXXXXXXXXYMGLDFNTPTQXXXX 388
            FGS+ S P+S+   G   +T + + +   GL FG                F+ PT
Sbjct: 1511 FGSSSSTPSSTGLFGSSSSTTSSTTTPSTGL-FGSAAPS-------TSSPFSIPTSSTPA 1562

Query: 389  XXXXYGLDFGSTP---SKPENSSSNYMGLDFNTPSQSSSSNNYGLDFGAP 529
                    FGS P   S P   SS      F  PS S+S+++  L FGAP
Sbjct: 1563 TSN----PFGSNPFPTSSPTTVSSTPSSNPFGAPSLSNSTSSSSL-FGAP 1607

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 648,950,026
Number of extensions: 12456361
Number of successful extensions: 21068
Number of sequences better than 10.0: 2
Number of HSP's gapped: 21009
Number of HSP's successfully gapped: 2
Length of query: 209
Length of database: 1,051,180,864
Length adjustment: 123
Effective length of query: 86
Effective length of database: 653,084,107
Effective search space: 56165233202
Effective search space used: 56165233202
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U13574-1 is cyt
VS (DIR, S) 0
VH (FL, L) 1
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0