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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U13424-1
Contig ID Contig-U13424-1
Contig update 2002.12.18
Contig sequence 
>Contig-U13424-1 (Contig-U13424-1Q) /CSM_Contig/Contig-U13424-1Q.Seq.d
TTCAATTGGGTACAGGTGTTGAATGTTTTCAAAAAGCAGTTGCAGCACTC
AAACAATGGAAACACTTTGATTTAGATTGGGTAGATTTTTACTTTAAAAA
TACACCTATTGCAGTGGGTGAAACCGTTGGAATCTTGTCAAAGCAAGTTG
GTTTTTGGATTCTATCATTTGCTCGTATCAATTATCTTTACGATGGTGAT
CAAGAAGATGGTTCAATTAAATTTGGTTATTCATATGGTACACTAAAGGA
TCATGTTGAAAAAGGTGAAGAAAGATTCGTCATTGAATGGGTACGTGATC
CTGATGGTGCCCCTGACAAAGGTGCTGTTTATTATGAAATGTTATCCTTT
TCAGAACCAAGCTATTGGTTATCTCAATTGGGTTATCCTGTAACTCGTTA
CTTTCAAAATAAATTTGTTGTTGATTCATGTAATCAAATGTTAAAAGCAG
TGGGTTCAAATCAAAATGTTAGACATGTATAATTAAGTATTATTAAAATA
ATAATAAAATAAATAAATAAAAAAAAATAAAATAAAATAAATTATAAATT
TATTAAAAATAAAAAAAGA
Gap no gap
Contig length 569
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 898474
End point 897906
Strand (PLUS/MINUS) MINUS
Number of clones 2
Number of EST 2
Link to clone list U13424
List of clone(s) 

est1=SSI354Z,1,560
est2=SSA248Z,71,569
Translated Amino Acid sequence 
QLGTGVECFQKAVAALKQWKHFDLDWVDFYFKNTPIAVGETVGILSKQVGFWILSFARIN
YLYDGDQEDGSIKFGYSYGTLKDHVEKGEERFVIEWVRDPDGAPDKGAVYYEMLSFSEPS
YWLSQLGYPVTRYFQNKFVVDSCNQMLKAVGSNQNVRHV*lsiikiiik*inkkk*nkin
ykfiknkkr
Translated Amino Acid sequence (All Frames) 
Frame A:
fnwvqvlnvfkkqlqhsnngntli*ig*iftlkihllqwvkplescqsklvfgfyhllvs
iiftmvikkmvqlnlvihmvh*rimlkkvkkdsslngyvilmvpltkvlfimkcypfqnq
aigylnwvil*lvtfkinlllihvikc*kqwvqikmldmyn*vllk***nk*ikknkik*
iinllkikk


Frame B:
sigyrc*mfsksscstqtmetl*frlgrfll*kytycsg*nrwnlvkaswfldsiicsyq
lslrw*srrwfn*iwlfiwytkgsc*kr*rkirh*mgt*s*wcp*qrccll*nvilfrtk
llvisiglscnsllsk*icc*fm*snvkssgfkskc*tciikyy*nnnkink*kkik*nk
l*iy*k*kk


Frame C:
QLGTGVECFQKAVAALKQWKHFDLDWVDFYFKNTPIAVGETVGILSKQVGFWILSFARIN
YLYDGDQEDGSIKFGYSYGTLKDHVEKGEERFVIEWVRDPDGAPDKGAVYYEMLSFSEPS
YWLSQLGYPVTRYFQNKFVVDSCNQMLKAVGSNQNVRHV*lsiikiiik*inkkk*nkin
ykfiknkkr
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U13424-1 (Contig-U13424-1Q)
/CSM_Contig/Contig-U13424-1Q.Seq.d
         (569 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U13424-1 (Contig-U13424-1Q) /CSM_Contig/Conti...   977  0.0
Contig-U03792-1 (Contig-U03792-1Q) /CSM_Contig/Conti...    40  0.003
Contig-U10836-1 (Contig-U10836-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U11788-1 (Contig-U11788-1Q) /CSM_Contig/Conti...    34  0.16
Contig-U11669-1 (Contig-U11669-1Q) /CSM_Contig/Conti...    34  0.16
Contig-U01791-1 (Contig-U01791-1Q) /CSM_Contig/Conti...    34  0.16
Contig-U14686-1 (Contig-U14686-1Q) /CSM_Contig/Conti...    32  0.62
Contig-U14188-1 (Contig-U14188-1Q) /CSM_Contig/Conti...    32  0.62
Contig-U11804-1 (Contig-U11804-1Q) /CSM_Contig/Conti...    32  0.62
Contig-U11323-1 (Contig-U11323-1Q) /CSM_Contig/Conti...    32  0.62

>Contig-U13424-1 (Contig-U13424-1Q) /CSM_Contig/Contig-U13424-1Q.Seq.d
          Length = 569

 Score =  977 bits (493), Expect = 0.0
 Identities = 493/493 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   ttcaattgggtacaggtgttgaatgttttcaaaaagcagttgcagcactcaaacaatgga 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   ttcaattgggtacaggtgttgaatgttttcaaaaagcagttgcagcactcaaacaatgga 60

                                                                       
Query: 61  aacactttgatttagattgggtagatttttactttaaaaatacacctattgcagtgggtg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aacactttgatttagattgggtagatttttactttaaaaatacacctattgcagtgggtg 120

                                                                       
Query: 121 aaaccgttggaatcttgtcaaagcaagttggtttttggattctatcatttgctcgtatca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aaaccgttggaatcttgtcaaagcaagttggtttttggattctatcatttgctcgtatca 180

                                                                       
Query: 181 attatctttacgatggtgatcaagaagatggttcaattaaatttggttattcatatggta 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 attatctttacgatggtgatcaagaagatggttcaattaaatttggttattcatatggta 240

                                                                       
Query: 241 cactaaaggatcatgttgaaaaaggtgaagaaagattcgtcattgaatgggtacgtgatc 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 cactaaaggatcatgttgaaaaaggtgaagaaagattcgtcattgaatgggtacgtgatc 300

                                                                       
Query: 301 ctgatggtgcccctgacaaaggtgctgtttattatgaaatgttatccttttcagaaccaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 ctgatggtgcccctgacaaaggtgctgtttattatgaaatgttatccttttcagaaccaa 360

                                                                       
Query: 361 gctattggttatctcaattgggttatcctgtaactcgttactttcaaaataaatttgttg 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 gctattggttatctcaattgggttatcctgtaactcgttactttcaaaataaatttgttg 420

                                                                       
Query: 421 ttgattcatgtaatcaaatgttaaaagcagtgggttcaaatcaaaatgttagacatgtat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 ttgattcatgtaatcaaatgttaaaagcagtgggttcaaatcaaaatgttagacatgtat 480

                        
Query: 481 aattaagtattat 493
           |||||||||||||
Sbjct: 481 aattaagtattat 493


>Contig-U03792-1 (Contig-U03792-1Q) /CSM_Contig/Contig-U03792-1Q.Seq.d
          Length = 833

 Score = 40.1 bits (20), Expect = 0.003
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                   
Query: 211 gttcaattaaatttggttattcat 234
           ||||||||||||||||| ||||||
Sbjct: 312 gttcaattaaatttggtaattcat 335


>Contig-U10836-1 (Contig-U10836-1Q) /CSM_Contig/Contig-U10836-1Q.Seq.d
          Length = 2417

 Score = 36.2 bits (18), Expect = 0.040
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                  
Query: 194  tggtgatcaagaagatggttca 215
            |||||||||| |||||||||||
Sbjct: 1785 tggtgatcaaaaagatggttca 1806


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 6071
Number of Sequences: 6905
Number of extensions: 6071
Number of successful extensions: 582
Number of sequences better than 10.0: 186
length of query: 569
length of database: 5,674,871
effective HSP length: 16
effective length of query: 553
effective length of database: 5,564,391
effective search space: 3077108223
effective search space used: 3077108223
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.12. 9
Homology vs DNA 
Query= Contig-U13424-1 (Contig-U13424-1Q) /CSM_Contig/Contig-U13424-1Q.Seq.d
         (569 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C90396) Dictyostelium discoideum slug cDNA, clone SSI354.        977   0.0    1
(AC115594) Dictyostelium discoideum chromosome 2 map 4071862...   971   0.0    1
(C25601) Dictyostelium discoideum slug cDNA, clone SSA248.        833   0.0    1
(BX950186) Zebrafish DNA sequence from clone CH211-103O18 in...    38   0.26   4
(AM477414) Vitis vinifera contig VV78X278831.16, whole genom...    48   0.34   1
(EI451167) PV_GBa0050M05.f PV_GBa Phaseolus vulgaris genomic...    48   0.34   1
(EI439292) PV_GBa0034G18.f PV_GBa Phaseolus vulgaris genomic...    48   0.34   1
(EI436825) PV_GBa0030P15.r PV_GBa Phaseolus vulgaris genomic...    48   0.34   1
(CX017873) EST 1582 Wild type Ugni blanc SSH Library Vitis v...    48   0.34   1
(BQ299169) sao43h10.y1 Gm-c1054 Glycine max cDNA clone SOYBE...    48   0.34   1
(AQ883067) HS_5456_A2_B09_SP6E RPCI-11 Human Male BAC Librar...    42   0.45   2
(AC179195) Strongylocentrotus purpuratus clone R3-4019F1, WO...    38   2.4    2
(AC171920) Bos taurus clone CH240-456I3, WORKING DRAFT SEQUE...    32   2.7    2
(AC223953) Bos taurus clone CH240-453F23, WORKING DRAFT SEQU...    32   2.7    2
(AM456885) Vitis vinifera contig VV78X112375.12, whole genom...    34   2.9    2
(DQ223181) Silurus meridionalis clone HLJCF28 microsatellite...    44   5.4    1
(AY924391) Bear Canyon virus strain AV A0070039 glycoprotein...    44   5.4    1
(AF512833) Bear canyon virus strain A0060209 glycoprotein pr...    44   5.4    1
(AM432684) Vitis vinifera contig VV78X063283.6, whole genome...    44   5.4    1
(AC118480) Homo sapiens BAC clone RP11-393K13 from 4, comple...    44   5.4    1
(AC110323) Rattus norvegicus clone CH230-254A20, WORKING DRA...    44   5.4    1
(AC199925) Zea mays chromosome 8 clone CH201-247P3; ZMMBBc02...    44   5.4    1
(AC199044) Zea mays chromosome 8 clone ZMMBBb-314I9; ZMMBBb0...    44   5.4    1
(AC184909) Bos taurus clone CH240-81I17, WORKING DRAFT SEQUE...    44   5.4    1
(AC172438) Bos taurus clone CH240-524B17, WORKING DRAFT SEQU...    44   5.4    1
(CZ720810) OC__Ba0046B22.f OC__Ba Oryza coarctata genomic cl...    44   5.4    1
(CZ276030) ZMMBF0058D19f ZMMBF Zea mays genomic clone ZMMBF0...    44   5.4    1
(CW855721) she3g2-9.g_005.ab1 Whole-genome shotgun library o...    44   5.4    1
(AG651523) Macaca fuscata fuscata DNA, clone: MSB2-066A19_R,...    44   5.4    1
(CU383740) Microbotryum violaceum EST isolate S.latifolia.         44   5.4    1
(CU383415) Microbotryum violaceum EST isolate S.latifolia.         44   5.4    1
(CU379733) Microbotryum violaceum EST isolate S.latifolia.         44   5.4    1
(CU376633) Microbotryum violaceum EST isolate S.latifolia.         44   5.4    1
(BM336672) MEST197-C08.T3 ISUM5-RN Zea mays cDNA clone MEST1...    44   5.4    1
(BM335818) MEST161-G02.T3 ISUM5-RN Zea mays cDNA clone MEST1...    44   5.4    1
(FC738006) CBBH2781.fwd CBBH Lottia gigantea 26h,37h,61h Lar...    32   5.4    3
(AC209790) MACACA MULATTA BAC clone CH250-536L15 from chromo...    38   5.6    2
(AC177307) Strongylocentrotus purpuratus clone R3-46C10, WOR...    38   8.1    3
(BX511189) Zebrafish DNA sequence from clone DKEY-28G23 in l...    38   8.2    4
(FC764431) CBBN14559.fwd CBBN Lottia gigantea 3,4,5,6.5d Lar...    32   8.4    3
(AC189219) Brassica rapa subsp. pekinensis clone KBrB010F19,...    36   9.2    3
(CO467471) MZCCL20009B12.g Maize Endosperm cDNA Library Zea ...    38   9.5    2
(AC102551) Mus musculus chromosome 16, clone RP23-154N19, co...    30   9.5    2
(AL831751) Mouse DNA sequence *** SEQUENCING CANCELLED *** f...    30   9.5    2
(CT010568) Mouse DNA sequence from clone RP23-290F22 on chro...    30   9.6    2
(AC146240) Pan troglodytes chromosome UNK clone RP43-27L15, ...    40   9.6    3
(AL670290) Mouse DNA sequence from clone RP23-309M8 on chrom...    30   9.7    2
(EJ281726) 1095366057534 Global-Ocean-Sampling_GS-27-01-01-1...    40   9.9    2

>(C90396) Dictyostelium discoideum slug cDNA, clone SSI354.
          Length = 560

 Score =  977 bits (493), Expect = 0.0
 Identities = 493/493 (100%)
 Strand = Plus / Plus


Query: 1   ttcaattgggtacaggtgttgaatgttttcaaaaagcagttgcagcactcaaacaatgga 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   ttcaattgggtacaggtgttgaatgttttcaaaaagcagttgcagcactcaaacaatgga 60


Query: 61  aacactttgatttagattgggtagatttttactttaaaaatacacctattgcagtgggtg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aacactttgatttagattgggtagatttttactttaaaaatacacctattgcagtgggtg 120


Query: 121 aaaccgttggaatcttgtcaaagcaagttggtttttggattctatcatttgctcgtatca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aaaccgttggaatcttgtcaaagcaagttggtttttggattctatcatttgctcgtatca 180


Query: 181 attatctttacgatggtgatcaagaagatggttcaattaaatttggttattcatatggta 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 attatctttacgatggtgatcaagaagatggttcaattaaatttggttattcatatggta 240


Query: 241 cactaaaggatcatgttgaaaaaggtgaagaaagattcgtcattgaatgggtacgtgatc 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 cactaaaggatcatgttgaaaaaggtgaagaaagattcgtcattgaatgggtacgtgatc 300


Query: 301 ctgatggtgcccctgacaaaggtgctgtttattatgaaatgttatccttttcagaaccaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 ctgatggtgcccctgacaaaggtgctgtttattatgaaatgttatccttttcagaaccaa 360


Query: 361 gctattggttatctcaattgggttatcctgtaactcgttactttcaaaataaatttgttg 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 gctattggttatctcaattgggttatcctgtaactcgttactttcaaaataaatttgttg 420


Query: 421 ttgattcatgtaatcaaatgttaaaagcagtgggttcaaatcaaaatgttagacatgtat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 ttgattcatgtaatcaaatgttaaaagcagtgggttcaaatcaaaatgttagacatgtat 480


Query: 481 aattaagtattat 493
           |||||||||||||
Sbjct: 481 aattaagtattat 493

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 609,163,033
Number of extensions: 35358177
Number of successful extensions: 2888800
Number of sequences better than 10.0: 48
Length of query: 569
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 546
Effective length of database: 93,106,754,628
Effective search space: 50836288026888
Effective search space used: 50836288026888
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 7
Homology vs Protein 
Query= Contig-U13424-1 (Contig-U13424-1Q) /CSM_Contig/Contig-U13424-1Q.Seq.d
         (569 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM746676_917(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   102   7e-21
AC150705_19(AC150705|pid:none) Medicago truncatula clone mth2-13...    93   4e-18
BX294137_316(BX294137|pid:none) Rhodopirellula baltica SH 1 comp...    93   6e-18
BT052408_1(BT052408|pid:none) Medicago truncatula clone MTYFD_FE...    92   7e-18
CP000589_58(CP000589|pid:none) Ostreococcus lucimarinus CCE9901 ...    89   8e-17
CR954209_54(CR954209|pid:none) Ostreococcus tauri strain OTTH059...    87   2e-16
AK071651_1(AK071651|pid:none) Oryza sativa Japonica Group cDNA c...    82   8e-15
AK101392_1(AK101392|pid:none) Oryza sativa Japonica Group cDNA c...    82   8e-15
AC113930_4(AC113930|pid:none) Oryza sativa (japonica cultivar-gr...    82   8e-15
CR855034_6(CR855034|pid:none) Oryza sativa (indica cultivar-grou...    81   2e-14
EF086953_1(EF086953|pid:none) Picea sitchensis clone WS02738_N12...    79   1e-13
BT038043_1(BT038043|pid:none) Zea mays full-length cDNA clone ZM...    77   4e-13
CU458896_2878(CU458896|pid:none) Mycobacterium abscessus chromos...    63   6e-09
AP009044_1637(AP009044|pid:none) Corynebacterium glutamicum R DN...    62   1e-08
AE016958_2721(AE016958|pid:none) Mycobacterium avium subsp. para...    60   5e-08
BX927152_219(BX927152|pid:none) Corynebacterium glutamicum ATCC ...    58   2e-07
CP000479_3374(CP000479|pid:none) Mycobacterium avium 104, comple...    57   3e-07
CP000431_1531(CP000431|pid:none) Rhodococcus jostii RHA1, comple...    55   2e-06
CP000854_2064(CP000854|pid:none) Mycobacterium marinum M, comple...    54   4e-06
AP011115_1257(AP011115|pid:none) Rhodococcus opacus B4 DNA, comp...    53   5e-06
AP006618_3252(AP006618|pid:none) Nocardia farcinica IFM 10152 DN...    53   7e-06
AP009493_729(AP009493|pid:none) Streptomyces griseus subsp. gris...    52   1e-05
AL939123_223(AL939123|pid:none) Streptomyces coelicolor A3(2) co...    49   7e-05
CP000656_3817(CP000656|pid:none) Mycobacterium gilvum PYR-GCK, c...    47   5e-04
AM849034_1720(AM849034|pid:none) Clavibacter michiganensis subsp...    46   6e-04
AM711867_1578(AM711867|pid:none) Clavibacter michiganensis subsp...    46   8e-04
AE016822_1172(AE016822|pid:none) Leifsonia xyli subsp. xyli str....    45   0.001
AE000516_2786(AE000516|pid:none) Mycobacterium tuberculosis CDC1...    45   0.002
AM408590_2647(AM408590|pid:none) Mycobacterium bovis BCG Pasteur...    45   0.002
BA000030_2802(BA000030|pid:none) Streptomyces avermitilis MA-468...    44   0.003
CP000667_1820(CP000667|pid:none) Salinispora tropica CNB-440, co...    42   0.009
CT573213_4954(CT573213|pid:none) Frankia alni str. ACN14A chromo...    41   0.020
CP000454_534(CP000454|pid:none) Arthrobacter sp. FB24, complete ...    40   0.044
CP001601_711(CP001601|pid:none) Corynebacterium aurimucosum ATCC...    37   0.28
BA000035_1669(BA000035|pid:none) Corynebacterium efficiens YS-31...    37   0.37
AP010904_3815(AP010904|pid:none) Desulfovibrio magneticus RS-1 D...    35   1.4
CU458896_3430(CU458896|pid:none) Mycobacterium abscessus chromos...    35   1.8
AJ293568_37(AJ293568|pid:none) Yaba-like disease virus (YLDV), c...    35   1.8
CR931997_1571(CR931997|pid:none) Corynebacterium jeikeium K411 c...    35   1.8
BA000030_1089(BA000030|pid:none) Streptomyces avermitilis MA-468...    34   3.1
CP000875_2436(CP000875|pid:none) Herpetosiphon aurantiacus ATCC ...    33   4.1
AE001437_2423(AE001437|pid:none) Clostridium acetobutylicum ATCC...    33   4.1
EU016887_1(EU016887|pid:none) Elaeis oleifera maturase K (matK) ...    33   5.4
AE014186_446(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    33   7.0
CP000937_322(CP000937|pid:none) Francisella philomiragia subsp. ...    33   7.0
CP000792_1524(CP000792|pid:none) Campylobacter concisus 13826, c...    33   7.0
CP000975_1457(CP000975|pid:none) Methylacidiphilum infernorum V4...    33   7.0
EU004872_1(EU004872|pid:none) Astrocaryum mexicanum isolate ASME...    32   9.2
AL590450_52(AL590450|pid:none) chromosome XI of strain GB-M1 of ...    32   9.2
CP000580_407(CP000580|pid:none) Mycobacterium sp. JLS, complete ...    32   9.2

>AM746676_917(AM746676|pid:none) Sorangium cellulosum 'So ce 56'
           complete genome.
          Length = 199

 Score =  102 bits (254), Expect = 7e-21
 Identities = 57/152 (37%), Positives = 87/152 (57%), Gaps = 1/152 (0%)
 Frame = +3

Query: 6   LGTGVECFQKAVAALKQWKHFDLDWVDFYFKNTPIAVGETVGILSKQVGFWILSFARINY 185
           LG G   F +AV A+++W  F L WV+  + + P+  G TV IL + +G   L+  RI
Sbjct: 54  LGQGTALFDRAVEAMRRWAQFRLGWVELCYPDAPLQAGVTVVILVRVLGIHWLNACRI-- 111

Query: 186 LYDGDQEDGSI-KFGYSYGTLKDHVEKGEERFVIEWVRDPDGAPDKGAVYYEMLSFSEPS 362
           +   D+  G + +FG++YGTL++H E+GEERF++E           G V Y++ + S P+
Sbjct: 112 VSTVDEASGPVRRFGFAYGTLEEHGERGEERFLVE------HDEHSGEVAYDIFAVSRPN 165

Query: 363 YWLSQLGYPVTRYFQNKFVVDSCNQMLKAVGS 458
           + L++LGYP  R  Q +F  DS   M  AV S
Sbjct: 166 HLLARLGYPYARRVQGRFARDSMGAMRSAVTS 197

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 865,136,628
Number of extensions: 17295116
Number of successful extensions: 41845
Number of sequences better than 10.0: 50
Number of HSP's gapped: 41788
Number of HSP's successfully gapped: 50
Length of query: 189
Length of database: 1,051,180,864
Length adjustment: 121
Effective length of query: 68
Effective length of database: 659,557,225
Effective search space: 44849891300
Effective search space used: 44849891300
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.75  gvh: 0.52  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U13424-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 2
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0