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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U13332-1
Contig ID Contig-U13332-1
Contig update 2002.12.18
Contig sequence 
>Contig-U13332-1 (Contig-U13332-1Q) /CSM_Contig/Contig-U13332-1Q.Seq.d
AGATAAGAAACTTTGGGATCTTTACATTTGGAAGATTACTCAAGCTTTAG
AGTCTTTAAGAAATGTTGTACAATTTGGAAAAGTCAATCCAAATCTTTAT
AATATGGCCGGTTCAAATCGTATGAGATTGTTATTCAGTTTCTTGGCTCA
TCAAGACAATCAAAAGATTCAAAAGCTTGCTTTAGAGTCATTGTTATTGG
TAACTTCCAATATTGAATGTATTAATCATATCTCACAATTGGCCACTGAA
CCAGAGGGTAGTTTACTTCCATACTTGTTACAACTTTTAGCAAGACCAAA
TCCTGCTACTTTGGAATTAGCATTAAAGGTAGAACATTCATTGGTTGCAA
TTTCTCAACACGTTATAGATACCATTAATCATTGTGGTTTAGTTTACTTA
TTGGATGTTTTCTCAAACAATCAAATTACAATGGAAAATCGTATTAAAGC
TGTTGGTGTTTTAGGTAGAATGATCATTGATAAAGTACATGGTCCAAGGG
TTATTCTATTAATTGGTAAATTCATTCCACCAGTTTTCAATTCAACAATG
ACAGAGGATCCGCAACAAACCGTTATGGTATTCGATGCTCAACATGAAAA
TCCAGAGTTAATTTGGAATAATAATACACGTGCCCACTTAAAAGAAACAC
TTACAAAGATGCAAAAAGAATA
Gap no gap
Contig length 672
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 4309799
End point 4309127
Strand (PLUS/MINUS) MINUS
Number of clones 4
Number of EST 4
Link to clone list U13332
List of clone(s) 

est1=SLD882F,1,272
est2=SLD896F,1,283
est3=VFH815F,85,603
est4=VFG638F,120,673
Translated Amino Acid sequence 
DKKLWDLYIWKITQALESLRNVVQFGKVNPNLYNMAGSNRMRLLFSFLAHQDNQKIQKLA
LESLLLVTSNIECINHISQLATEPEGSLLPYLLQLLARPNPATLELALKVEHSLVAISQH
VIDTINHCGLVYLLDVFSNNQITMENRIKAVGVLGRMIIDKVHGPRVILLIGKFIPPVFN
STMTEDPQQTVMVFDAQHENPELIWNNNTRAHLKETLTKMQKE
Translated Amino Acid sequence (All Frames) 
Frame A:
r*etlgslhledyssfrvfkkcctiwksqsksl*ygrfksyeiviqflgssrqskdskac
frvivignfqy*my*syltigh*trg*ftsilvttfsktkscyfgisikgrtfigcnfst
ryryh*slwfslligcflkqsnyngksy*scwcfr*ndh**stwskgysinw*ihstsfq
fnndrgsatnrygircst*ksrvnle**ytcplkrntykdakri


Frame B:
DKKLWDLYIWKITQALESLRNVVQFGKVNPNLYNMAGSNRMRLLFSFLAHQDNQKIQKLA
LESLLLVTSNIECINHISQLATEPEGSLLPYLLQLLARPNPATLELALKVEHSLVAISQH
VIDTINHCGLVYLLDVFSNNQITMENRIKAVGVLGRMIIDKVHGPRVILLIGKFIPPVFN
STMTEDPQQTVMVFDAQHENPELIWNNNTRAHLKETLTKMQKE


Frame C:
irnfgiftfgrllkl*sl*emlynleksiqifiiwpvqiv*dcysvswliktikrfksll
*shcyw*lpilnvliishnwplnqrvvyfhtcynf*qdqilllwn*h*r*nihwlqflnt
l*ipliivv*ftywmfsqtiklqwkivlkllvf*ve*slikymvqglfy*lvnsfhqfsi
qq*qrirnkplwysmlnmkiqs*fgiiihvpt*kkhlqrckkn
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U13332-1 (Contig-U13332-1Q)
/CSM_Contig/Contig-U13332-1Q.Seq.d
         (672 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U13332-1 (Contig-U13332-1Q) /CSM_Contig/Conti...  1332  0.0
Contig-U13753-1 (Contig-U13753-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U11459-1 (Contig-U11459-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U10392-1 (Contig-U10392-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U14809-1 (Contig-U14809-1Q) /CSM_Contig/Conti...    32  0.73
Contig-U14660-1 (Contig-U14660-1Q) /CSM_Contig/Conti...    32  0.73
Contig-U14295-1 (Contig-U14295-1Q) /CSM_Contig/Conti...    32  0.73
Contig-U12228-1 (Contig-U12228-1Q) /CSM_Contig/Conti...    32  0.73
Contig-U11983-1 (Contig-U11983-1Q) /CSM_Contig/Conti...    32  0.73

>Contig-U13332-1 (Contig-U13332-1Q) /CSM_Contig/Contig-U13332-1Q.Seq.d
          Length = 672

 Score = 1332 bits (672), Expect = 0.0
 Identities = 672/672 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   agataagaaactttgggatctttacatttggaagattactcaagctttagagtctttaag 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   agataagaaactttgggatctttacatttggaagattactcaagctttagagtctttaag 60

                                                                       
Query: 61  aaatgttgtacaatttggaaaagtcaatccaaatctttataatatggccggttcaaatcg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aaatgttgtacaatttggaaaagtcaatccaaatctttataatatggccggttcaaatcg 120

                                                                       
Query: 121 tatgagattgttattcagtttcttggctcatcaagacaatcaaaagattcaaaagcttgc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tatgagattgttattcagtttcttggctcatcaagacaatcaaaagattcaaaagcttgc 180

                                                                       
Query: 181 tttagagtcattgttattggtaacttccaatattgaatgtattaatcatatctcacaatt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 tttagagtcattgttattggtaacttccaatattgaatgtattaatcatatctcacaatt 240

                                                                       
Query: 241 ggccactgaaccagagggtagtttacttccatacttgttacaacttttagcaagaccaaa 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 ggccactgaaccagagggtagtttacttccatacttgttacaacttttagcaagaccaaa 300

                                                                       
Query: 301 tcctgctactttggaattagcattaaaggtagaacattcattggttgcaatttctcaaca 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 tcctgctactttggaattagcattaaaggtagaacattcattggttgcaatttctcaaca 360

                                                                       
Query: 361 cgttatagataccattaatcattgtggtttagtttacttattggatgttttctcaaacaa 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 cgttatagataccattaatcattgtggtttagtttacttattggatgttttctcaaacaa 420

                                                                       
Query: 421 tcaaattacaatggaaaatcgtattaaagctgttggtgttttaggtagaatgatcattga 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 tcaaattacaatggaaaatcgtattaaagctgttggtgttttaggtagaatgatcattga 480

                                                                       
Query: 481 taaagtacatggtccaagggttattctattaattggtaaattcattccaccagttttcaa 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 taaagtacatggtccaagggttattctattaattggtaaattcattccaccagttttcaa 540

                                                                       
Query: 541 ttcaacaatgacagaggatccgcaacaaaccgttatggtattcgatgctcaacatgaaaa 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ttcaacaatgacagaggatccgcaacaaaccgttatggtattcgatgctcaacatgaaaa 600

                                                                       
Query: 601 tccagagttaatttggaataataatacacgtgcccacttaaaagaaacacttacaaagat 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 tccagagttaatttggaataataatacacgtgcccacttaaaagaaacacttacaaagat 660

                       
Query: 661 gcaaaaagaata 672
           ||||||||||||
Sbjct: 661 gcaaaaagaata 672


>Contig-U13753-1 (Contig-U13753-1Q) /CSM_Contig/Contig-U13753-1Q.Seq.d
          Length = 663

 Score = 36.2 bits (18), Expect = 0.047
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 158 aatcaaaagattcaaaagcttg 179
           ||||||||||||||||| ||||
Sbjct: 513 aatcaaaagattcaaaaacttg 534


>Contig-U11459-1 (Contig-U11459-1Q) /CSM_Contig/Contig-U11459-1Q.Seq.d
          Length = 1946

 Score = 36.2 bits (18), Expect = 0.047
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                  
Query: 158  aatcaaaagattcaaaagcttg 179
            ||||||||||||||||| ||||
Sbjct: 1793 aatcaaaagattcaaaaacttg 1814


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 6385
Number of Sequences: 6905
Number of extensions: 6385
Number of successful extensions: 573
Number of sequences better than 10.0: 57
length of query: 672
length of database: 5,674,871
effective HSP length: 16
effective length of query: 656
effective length of database: 5,564,391
effective search space: 3650240496
effective search space used: 3650240496
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.12. 7
Homology vs DNA 
Query= Contig-U13332-1 (Contig-U13332-1Q) /CSM_Contig/Contig-U13332-1Q.Seq.d
         (672 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ413156) Dictyostelium discoideum cDNA clone:ddv14k10, 5' ...   874   0.0    2
(BJ413552) Dictyostelium discoideum cDNA clone:ddv16n04, 5' ...   829   0.0    3
(AU061396) Dictyostelium discoideum slug cDNA, clone SLD896.      561   e-155  1
(AU061389) Dictyostelium discoideum slug cDNA, clone SLD882.      539   e-149  1
(EG610177) KIDNEYF100760M6 POSSUM_01-POSSUM-KIDNEY-2KB Trich...    54   0.007  1
(EC354599) LIVERF089697J9 POSSUM_01-POSSUM-LIVER-2KB Trichos...    54   0.007  1
(EC351471) LIVERF091688E7 POSSUM_01-POSSUM-LIVER-2KB Trichos...    54   0.007  1
(EC297548) OVARYF092731J1 POSSUM_01-POSSUM-C-OVARY-2KB Trich...    54   0.007  1
(CN226455) RJB048B07.ab1 RJtestis Gallus gallus cDNA 5', mRN...    54   0.007  1
(BH168262) VV_SBa0002E04r Grape Vitis vinifera genomic clone...    44   0.080  2
(DD178487) NUCLEIC ACID-ASSOCIATED PROTEINS.                       50   0.11   1
(DD145300) Angiogenesis_PCT.                                       50   0.11   1
(DD035289) Full length cDNA.                                       50   0.11   1
(CS351157) Sequence 541 from Patent WO2006013561.                  50   0.11   1
(CQ850032) Sequence 501 from Patent EP1447413.                     50   0.11   1
(CQ842372) Sequence 1019 from Patent EP1440981.                    50   0.11   1
(CQ714980) Sequence 914 from Patent WO02068579.                    50   0.11   1
(BD159840) Primer for synthesizing full-length cDNA and use ...    50   0.11   1
(AX882636) Sequence 17541 from Patent EP1074617.                   50   0.11   1
(AR699080) Sequence 166 from patent US 6919193.                    50   0.11   1
(AR541918) Sequence 166 from patent US 6743619.                    50   0.11   1
(BC151246) Homo sapiens DnaJ (Hsp40) homolog, subfamily C, m...    50   0.11   1
(BC009630) Homo sapiens DnaJ (Hsp40) homolog, subfamily C, m...    50   0.11   1
(BC000164) Homo sapiens DnaJ (Hsp40) homolog, subfamily C, m...    50   0.11   1
(AY779857) Homo sapiens DnaJ domain-containing protein RME-8...    50   0.11   1
(AK127112) Homo sapiens cDNA FLJ45169 fis, clone BRAWH3045118.     50   0.11   1
(AK125330) Homo sapiens cDNA FLJ43340 fis, clone NT2RI3007543.     50   0.11   1
(AK023168) Homo sapiens cDNA FLJ13106 fis, clone NT2RP300245...    50   0.11   1
(AC020632) Homo sapiens 3 BAC RP11-333H9 (Roswell Park Cance...    50   0.11   1
(AB014578) Homo sapiens mRNA for KIAA0678 protein, partial cds.    50   0.11   1
(BC033782) Homo sapiens cDNA clone IMAGE:4500158, containing...    50   0.11   1
(DQ030864) Pan troglodytes HC604 gene, VIRTUAL TRANSCRIPT, p...    50   0.11   1
(DQ030863) Homo sapiens HC604 gene, VIRTUAL TRANSCRIPT, part...    50   0.11   1
(AU135807) Homo sapiens cDNA clone:PLACE1002943, 5' end, exp...    50   0.11   1
(AU124105) Homo sapiens cDNA clone:NT2RM2001673, 5' end, exp...    50   0.11   1
(DA822531) Homo sapiens cDNA clone PERIC2002807, 5' end, mRN...    50   0.11   1
(DA812442) Homo sapiens cDNA clone OVARC1001475, 5' end, mRN...    50   0.11   1
(DA737127) Homo sapiens cDNA clone NT2RP4000339, 5' end, mRN...    50   0.11   1
(DA689154) Homo sapiens cDNA clone NT2NE2008197, 5' end, mRN...    50   0.11   1
(DA616223) Homo sapiens cDNA clone IMR322015621, 5' end, mRN...    50   0.11   1
(DA548085) Homo sapiens cDNA clone HCASM2006328, 5' end, mRN...    50   0.11   1
(DA540685) Homo sapiens cDNA clone FELNG2000619, 5' end, mRN...    50   0.11   1
(CD251085) AGENCOURT_14214274 NIH_MGC_179 Homo sapiens cDNA ...    50   0.11   1
(BQ427595) AGENCOURT_7917321 NIH_MGC_72 Homo sapiens cDNA cl...    50   0.11   1
(BQ425707) AGENCOURT_7892709 NIH_MGC_72 Homo sapiens cDNA cl...    50   0.11   1
(BM829788) K-EST0102948 S21SNU520s1 Homo sapiens cDNA clone ...    50   0.11   1
(BM471286) AGENCOURT_6478236 NIH_MGC_72 Homo sapiens cDNA cl...    50   0.11   1
(BI850831) imageqc_1_2001/smg350bdrr81.y1 NIH_MGC_93 Homo sa...    50   0.11   1
(BG623752) 602648873F1 NIH_MGC_79 Homo sapiens cDNA clone IM...    50   0.11   1
(BG286888) 602382760F1 NIH_MGC_93 Homo sapiens cDNA clone IM...    50   0.11   1
(BG284201) 602408252F1 NIH_MGC_91 Homo sapiens cDNA clone IM...    50   0.11   1
(BG188569) RST7594 Athersys RAGE Library Homo sapiens cDNA, ...    50   0.11   1
(BG110848) 602278776F1 NIH_MGC_86 Homo sapiens cDNA clone IM...    50   0.11   1
(AA772930) ae73d07.s1 Stratagene schizo brain S11 Homo sapie...    50   0.11   1
(BF691361) 602247409F1 NIH_MGC_62 Homo sapiens cDNA clone IM...    50   0.11   1
(BE613936) 601504056F1 NIH_MGC_71 Homo sapiens cDNA clone IM...    50   0.11   1
(AW674827) ba59b03.y1 NIH_MGC_10 Homo sapiens cDNA clone IMA...    50   0.11   1
(FC121923) 1106878596610 BABEVPN-C-01-1-7KB Papio anubis cDN...    50   0.11   1
(AC112033) Rattus norvegicus clone CH230-94G2, *** SEQUENCIN...    46   1.7    1
(CU463338) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.7    1
(AQ407322) HS_5092_A2_H11_T7A RPCI-11 Human Male BAC Library...    46   1.7    1
(ER465206) 1092963919540 Global-Ocean-Sampling_GS-35-01-01-1...    46   1.7    1
(EK275747) 1095462276212 Global-Ocean-Sampling_GS-31-01-01-1...    46   1.7    1
(EE808343) 010716ONDC003051HT ONDC Ovis aries cDNA, mRNA seq...    46   1.7    1
(DV691750) CGN-35510 Leaf Coffea canephora cDNA clone cccl12...    46   1.7    1
(DV685981) CGN-27498 Leaf Coffea canephora cDNA clone cccl7p...    46   1.7    1
(DR413948) gPGC_EST03790 Embryonic gonadal PGC cDNA Library ...    46   1.7    1
(CV865311) PDUts1016D08 Porcine testis cDNA library I Sus sc...    46   1.7    1
(BX926827) Sus Scrofa library (scan) clone scan0015.j.11, 5p...    46   1.7    1
(BU136651) 603125408F1 CSEQCHL23 Gallus gallus cDNA clone Ch...    46   1.7    1
(BQ484986) pmp1c.pk007.k13 Chicken Macrophage cDNA library G...    46   1.7    1
(BM821274) K-EST0090270 S21SNU520s1 Homo sapiens cDNA clone ...    46   1.7    1
(Z48638) Caenorhabditis elegans Cosmid ZK892.                      32   3.5    6
(AC176113) Strongylocentrotus purpuratus clone R3-24J15, WOR...    40   5.7    2
(AC096637) Homo sapiens chromosome 1 clone RP11-122M14, comp...    38   6.4    3
(BV386872) S244P6258FA8.T0 AlaskaGrayWolf Canis lupus STS ge...    44   6.7    1
(AC162899) Mus musculus chromosome 1, clone RP24-222G3, comp...    44   6.7    1
(AC101787) Mus musculus chromosome 1, clone RP24-147F6, comp...    44   6.7    1
(AC008016) Arabidopsis thaliana chromosome 1 BAC F6D8 sequen...    44   6.7    1
(AC097378) Homo sapiens BAC clone RP11-123G5 from 4, complet...    44   6.7    1
(AC068057) Homo sapiens BAC clone CTD-2015C24 from 2, comple...    44   6.7    1
(AC154946) Bos taurus clone CH240-35D11, WORKING DRAFT SEQUE...    44   6.7    1
(AC079067) Homo sapiens chromosome 4 clone RP11-77P11 map 4,...    44   6.7    1
(AC216573) Triticum aestivum clone TAACSPALLhA_1177N07, *** ...    44   6.7    1
(AC185077) Bos taurus clone CH240-191M4, WORKING DRAFT SEQUE...    44   6.7    1
(AC164851) Bos taurus clone CH240-149C7, *** SEQUENCING IN P...    44   6.7    1
(EJ935383) 1093018835499 Global-Ocean-Sampling_GS-30-02-01-1...    44   6.7    1
(EJ039448) 1095454084930 Global-Ocean-Sampling_GS-26-01-01-1...    44   6.7    1
(ED996114) MUGQ_CH252P108F13Sp6_CN53_059 CHORI-252 Vervet Mo...    44   6.7    1
(ED555541) MUGQ_CH252P133C15T7_CN115_062 CHORI-252 Vervet Mo...    44   6.7    1
(AG605526) Mus musculus molossinus DNA, clone:MSMg01-538O13....    44   6.7    1
(CC886063) SALK_148165.39.40.x Arabidopsis thaliana TDNA ins...    44   6.7    1
(BZ596392) SALK_092493.37.65.x Arabidopsis thaliana TDNA ins...    44   6.7    1
(EH181162) LB01755.CR_I05 GC_BGC-17 Bos taurus cDNA clone IM...    44   6.7    1
(DV914547) LB02830.CR_O03 GC_BGC-28 Bos taurus cDNA clone IM...    44   6.7    1
(DT811001) LB01613.CR_M06 GC_BGC-16 Bos taurus cDNA clone IM...    44   6.7    1
(BU485500) 603469621F1 CSEQRBN22 Gallus gallus cDNA clone Ch...    44   6.7    1
(BE480311) 165355 BARC 5BOV Bos taurus cDNA 5', mRNA sequence.     44   6.7    1
(AC093536) Homo sapiens chromosome 16 clone RP11-744D14, com...    34   9.7    5

>(BJ413156) Dictyostelium discoideum cDNA clone:ddv14k10, 5' end,
           single read.
          Length = 548

 Score =  874 bits (441), Expect(2) = 0.0
 Identities = 441/441 (100%)
 Strand = Plus / Plus


Query: 232 ctcacaattggccactgaaccagagggtagtttacttccatacttgttacaacttttagc 291
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 107 ctcacaattggccactgaaccagagggtagtttacttccatacttgttacaacttttagc 166


Query: 292 aagaccaaatcctgctactttggaattagcattaaaggtagaacattcattggttgcaat 351
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 167 aagaccaaatcctgctactttggaattagcattaaaggtagaacattcattggttgcaat 226


Query: 352 ttctcaacacgttatagataccattaatcattgtggtttagtttacttattggatgtttt 411
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 227 ttctcaacacgttatagataccattaatcattgtggtttagtttacttattggatgtttt 286


Query: 412 ctcaaacaatcaaattacaatggaaaatcgtattaaagctgttggtgttttaggtagaat 471
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 287 ctcaaacaatcaaattacaatggaaaatcgtattaaagctgttggtgttttaggtagaat 346


Query: 472 gatcattgataaagtacatggtccaagggttattctattaattggtaaattcattccacc 531
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 347 gatcattgataaagtacatggtccaagggttattctattaattggtaaattcattccacc 406


Query: 532 agttttcaattcaacaatgacagaggatccgcaacaaaccgttatggtattcgatgctca 591
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 407 agttttcaattcaacaatgacagaggatccgcaacaaaccgttatggtattcgatgctca 466


Query: 592 acatgaaaatccagagttaatttggaataataatacacgtgcccacttaaaagaaacact 651
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 467 acatgaaaatccagagttaatttggaataataatacacgtgcccacttaaaagaaacact 526


Query: 652 tacaaagatgcaaaaagaata 672
           |||||||||||||||||||||
Sbjct: 527 tacaaagatgcaaaaagaata 547

 Score =  210 bits (106), Expect(2) = 0.0
 Identities = 106/106 (100%)
 Strand = Plus / Plus


Query: 120 gtatgagattgttattcagtttcttggctcatcaagacaatcaaaagattcaaaagcttg 179
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   gtatgagattgttattcagtttcttggctcatcaagacaatcaaaagattcaaaagcttg 60


Query: 180 ctttagagtcattgttattggtaacttccaatattgaatgtattaa 225
           ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  ctttagagtcattgttattggtaacttccaatattgaatgtattaa 106

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 751,858,680
Number of extensions: 41282542
Number of successful extensions: 3010594
Number of sequences better than 10.0: 99
Length of query: 672
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 648
Effective length of database: 97,308,875,965
Effective search space: 63056151625320
Effective search space used: 63056151625320
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 6
Homology vs Protein 
Query= Contig-U13332-1 (Contig-U13332-1Q) /CSM_Contig/Contig-U13332-1Q.Seq.d
         (672 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK125330_1(AK125330|pid:none) Homo sapiens cDNA FLJ43340 fis, cl...   114   2e-24
AK023168_1(AK023168|pid:none) Homo sapiens cDNA FLJ13106 fis, cl...   114   2e-24
T00361(T00361)hypothetical protein KIAA0678 - human (fragment)        114   2e-24
AY779857_1(AY779857|pid:none) Homo sapiens DnaJ domain-containin...   114   2e-24
BC151246_1(BC151246|pid:none) Homo sapiens DnaJ (Hsp40) homolog,...   114   2e-24
AB093258_1(AB093258|pid:none) Mus musculus mRNA for mKIAA0678 pr...   112   1e-23
AK142362_1(AK142362|pid:none) Mus musculus 13 days embryo lung c...   112   1e-23
T21087(T21087)hypothetical protein F18C12.2b - Caenorhabditis el...    89   2e-16
Z75536_4(Z75536|pid:none) Caenorhabditis elegans Cosmid F18C12, ...    89   2e-16
T21086(T21086)hypothetical protein F18C12.2a - Caenorhabditis el...    86   1e-15
AF372457_1(AF372457|pid:none) Caenorhabditis elegans endocytosis...    86   1e-15
BC009630_1(BC009630|pid:none) Homo sapiens DnaJ (Hsp40) homolog,...    85   2e-15
AC060755_24(AC060755|pid:none) Oryza sativa chromosome 10 BAC OS...    66   8e-10
CT005267_239(CT005267|pid:none) Leishmania major strain Friedlin...    50   5e-05
AC074258_11(AC074258|pid:none) Trypanosoma brucei chromosome 6 c...    48   3e-04
AM494967_216(AM494967|pid:none) Leishmania braziliensis chromoso...    44   0.004
EU002530_1(EU002530|pid:none) Phyllanthus calycinus RNA polymera...    33   7.7
(B1A925) RecName: Full=DNA-directed RNA polymerase subunit beta'...    33   7.7
EU002483_1(EU002483|pid:none) Carica papaya RNA polymerase beta'...    33   7.7

>AK125330_1(AK125330|pid:none) Homo sapiens cDNA FLJ43340 fis, clone
            NT2RI3007543.
          Length = 1375

 Score =  114 bits (286), Expect = 2e-24
 Identities = 67/213 (31%), Positives = 120/213 (56%)
 Frame = +2

Query: 32   KITQALESLRNVVQFGKVNPNLYNMAGSNRMRLLFSFLAHQDNQKIQKLALESLLLVTSN 211
            ++  ALE+LRNV+++   NP   +       +L+FS L      ++Q+LALE + +VTSN
Sbjct: 880  RVEMALEALRNVIKY---NPGSESEC-IGHFKLIFSLLRVHGAGQVQQLALEVVNIVTSN 935

Query: 212  IECINHISQLATEPEGSXXXXXXXXXARPNPATLELALKVEHSLVAISQHVIDTINHCGL 391
             +C+N+I++       S             P++ +L L+  ++L + ++ + + +    L
Sbjct: 936  QDCVNNIAE-------SMVLSSLLALLHSLPSSRQLVLETLYALTSSTKIIKEAMAKGAL 988

Query: 392  VYLLDVFSNNQITMENRIKAVGVLGRMIIDKVHGPRVILLIGKFIPPVFNSTMTEDPQQT 571
            +YLLD+F N+    + R +   +  +M  DK+ GP+V + + KF+P VF   M ++P+
Sbjct: 989  IYLLDMFCNSTHP-QVRAQTAELFAKMTADKLIGPKVRITLMKFLPSVFMDAMRDNPEAA 1047

Query: 572  VMVFDAQHENPELIWNNNTRAHLKETLTKMQKE 670
            V +F+  HENPELIWN+N+R  +  T+ +M  E
Sbjct: 1048 VHIFEGTHENPELIWNDNSRDKVSTTVREMMLE 1080

 Score = 35.4 bits (80), Expect = 1.6
 Identities = 18/53 (33%), Positives = 26/53 (49%)
 Frame = +2

Query: 509 LIGKFIPPVFNSTMTEDPQQTVMVFDAQHENPELIWNNNTRAHLKETLTKMQK 667
           L G   P V          + + + ++  E+P LIWNN+TRA L E L   Q+
Sbjct: 741 LAGMLTPYVARKLAVASVTEILKMLNSNTESPYLIWNNSTRAELLEFLESQQE 793

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,180,664,533
Number of extensions: 23881095
Number of successful extensions: 48006
Number of sequences better than 10.0: 19
Number of HSP's gapped: 47976
Number of HSP's successfully gapped: 27
Length of query: 224
Length of database: 1,051,180,864
Length adjustment: 124
Effective length of query: 100
Effective length of database: 649,847,548
Effective search space: 64984754800
Effective search space used: 64984754800
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U13332-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 2
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 2
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0