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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U13162-1
Contig ID Contig-U13162-1
Contig update 2002.12.18
Contig sequence 
>Contig-U13162-1 (Contig-U13162-1Q) /CSM_Contig/Contig-U13162-1Q.Seq.d
TATGCATGGGCAAGAAGATTAGATAACAATTATCCAGAGCATAAACATTT
TCAAGTTTCAATGTGGCAAGGATTGAAATTGGTTGGTATGGGATATAGAA
TGTGGAAATATGTAAAGAAAGAAACTTCCTCTGGAAGAGTACCAATTATG
GATCCATTCAATCTACCAAAACCTGGTCCAATCATGGGTGTACCCATTGG
TGGTATTGGTAGTGGTAGTATTAATCGTGGTTGGAAAGGTGATTTCGTTA
GATGGAATCTTAATAATGGTTTAGTTTCGAATGAAATCGTTGATGTTGAT
AAATTTTCAGTTTATATTAATTTTGATAATTCAAATACTTCATCATCATC
ATCATCATCATCATCAAATTCATCAACACCACCACCACAATTTTCAAATA
ATAATAATAACAGCTATAACAACAGCTATAACAATAACATTAATAATAAT
GGATTTGATAATAATTTAAAAAGTAAACAAAAAGCAGTTGTATTATATCC
AGGTAAACCAAAATCAAATTTAAATTTAGATGTTTGGAATTGGGGATTAA
AAGGTGATAATAGTTGTTATTTTGGTTTATTTTCCAAGAGCATGGACAGT
TTATGAAGAACCTCATCCTGATATTAGATTAACTTGTAAACAAGTTTCAC
CAGTCATACCTCACAATTATCAAGAATCTTCATATCCATGTGGTGTTTAT
GTTTGGAAAATTGAAAATAATAATCAATATTCATCCGCTGATGTTAGTTT
AATGTTAACTTGGCAAAATAGTATTGGTACTCAATCTGATCAAGATGGTG
GTCATTTTAATAAATATTTTACTTTTGGTGATAATGATCAACAATCACCA
TCATCATCATCATCATCATCATCTGGTAATAATAAAAAAAATATAAAAGG
TATTACTATGATTCATAAAAAAGAACAAAAATGTACATTACCAAATGGTA
ATACAAAGGTATTTAATGATCCAATGGAATATTCAATTGCAGTAATGGAT
GAACCAGATGTTGAAATTAGTTTTTGTGAAAGATTTGAAACTACTAGTAG
ATTGGATGCTGCAAATTTATGGTATACATTCAATAAGAAAGGTGAATTGG
AGAATAATAAAGATTCTAGACCATCAGCACCAAAGAAAC
Gap no gap
Contig length 1139
Chromosome number (1..6, M) 6
Chromosome length 3595308
Start point 1648211
End point 1649349
Strand (PLUS/MINUS) PLUS
Number of clones 5
Number of EST 5
Link to clone list U13162
List of clone(s) 

est1=VHG533F,1,586
est2=CHE890Z,183,772
est3=CHO220Z,270,566
est4=CHR748F,361,652
est5=VHO255F,658,1139
Translated Amino Acid sequence 
YAWARRLDNNYPEHKHFQVSMWQGLKLVGMGYRMWKYVKKETSSGRVPIMDPFNLPKPGP
IMGVPIGGIGSGSINRGWKGDFVRWNLNNGLVSNEIVDVDKFSVYINFDNSNTSSSSSSS
SSNSSTPPPQFSNNNNNSYNNSYNNNINNNGFDNNLKSKQKAVVLYPGKPKSNLNLDVWN
WGLKGDNSCYFGLFSKSMDSL*rtss*y*inl*tsftshtsqlsrifismwclclen*k*
*sifir*c*fnvnlak*ywysi*srwwsf**ifyfw***stitiiiiiiiiw***kkykr
yyyds*krtkmyitkw*ykgi**sngifncsng*trc*n*fl*ki*ny**igcckfmvyi
q*er*ige**rf*tistke
Translated Amino Acid sequence (All Frames) 
Frame A:
YAWARRLDNNYPEHKHFQVSMWQGLKLVGMGYRMWKYVKKETSSGRVPIMDPFNLPKPGP
IMGVPIGGIGSGSINRGWKGDFVRWNLNNGLVSNEIVDVDKFSVYINFDNSNTSSSSSSS
SSNSSTPPPQFSNNNNNSYNNSYNNNINNNGFDNNLKSKQKAVVLYPGKPKSNLNLDVWN
WGLKGDNSCYFGLFSKSMDSL*rtss*y*inl*tsftshtsqlsrifismwclclen*k*
*sifir*c*fnvnlak*ywysi*srwwsf**ifyfw***stitiiiiiiiiw***kkykr
yyyds*krtkmyitkw*ykgi**sngifncsng*trc*n*fl*ki*ny**igcckfmvyi
q*er*ige**rf*tistke


Frame B:
mhgqed*itiiqsinifkfqcgkd*nwlvwdiecgnm*rkklpleeyqlwihsiyqnlvq
swvyplvvlvvvvlivvgkvisldgilimv*frmkslmlinfqfililiiqilhhhhhhh
hqihqhhhhnfqiiiitaittaititliimdliii*kvnkkqlyyiqvnqnqi*i*mfgi
gd*kviivvilvyfprawtvyeephpdirltckqvspviphnyqessypcgvyvwkienn
nqyssadvslmltwqnsigtqsdqdgghfnkyftfgdndqqspssssssssgnnkknikg
itmihkkeqkctlpngntkvfndpmeysiavmdepdveisfcerfettsrldaanlwytf
nkkgelennkdsrpsapkk


Frame C:
cmgkkir*qlsra*tfssfnvarieigwygi*nveickernflwkstnygsiqstktwsn
hgcthwwyw*w*y*swler*fr*mes**wfsfe*nr*c**ifsly*f**fkyfiiiiiii
ikfinttttifk****ql*qql*q*h***wi***fkk*tkssciisr*tkikfkfrclel
gikr***llfwfifqehgqfmknlilild*lvnkfhqsyltiiknlhihvvfmfgklkii
inihplmlv*c*lgkivlvlnlikmvvilinilllviminnhhhhhhhhhlviikki*kv
ll*fikknknvhyqmviqrylmiqwniqlq*wmnqmlklvfvkdlkllvdwmlqiygihs
irkvnwriikildhqhqrn
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U13162-1 (Contig-U13162-1Q)
/CSM_Contig/Contig-U13162-1Q.Seq.d
         (1139 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U13162-1 (Contig-U13162-1Q) /CSM_Contig/Conti...   793  0.0
Contig-U14871-1 (Contig-U14871-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U14566-1 (Contig-U14566-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U14261-1 (Contig-U14261-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U12561-1 (Contig-U12561-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U01045-1 (Contig-U01045-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U12134-1 (Contig-U12134-1Q) /CSM_Contig/Conti...    38  0.020
Contig-U12019-1 (Contig-U12019-1Q) /CSM_Contig/Conti...    38  0.020
Contig-U11767-1 (Contig-U11767-1Q) /CSM_Contig/Conti...    38  0.020
Contig-U11441-1 (Contig-U11441-1Q) /CSM_Contig/Conti...    36  0.081

>Contig-U13162-1 (Contig-U13162-1Q) /CSM_Contig/Contig-U13162-1Q.Seq.d
          Length = 1139

 Score =  793 bits (400), Expect = 0.0
 Identities = 400/400 (100%)
 Strand = Plus / Plus

                                                                       
Query: 449 atggatttgataataatttaaaaagtaaacaaaaagcagttgtattatatccaggtaaac 508
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 449 atggatttgataataatttaaaaagtaaacaaaaagcagttgtattatatccaggtaaac 508

                                                                       
Query: 509 caaaatcaaatttaaatttagatgtttggaattggggattaaaaggtgataatagttgtt 568
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 509 caaaatcaaatttaaatttagatgtttggaattggggattaaaaggtgataatagttgtt 568

                                                                       
Query: 569 attttggtttattttccaagagcatggacagtttatgaagaacctcatcctgatattaga 628
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 569 attttggtttattttccaagagcatggacagtttatgaagaacctcatcctgatattaga 628

                                                                       
Query: 629 ttaacttgtaaacaagtttcaccagtcatacctcacaattatcaagaatcttcatatcca 688
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 629 ttaacttgtaaacaagtttcaccagtcatacctcacaattatcaagaatcttcatatcca 688

                                                                       
Query: 689 tgtggtgtttatgtttggaaaattgaaaataataatcaatattcatccgctgatgttagt 748
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 689 tgtggtgtttatgtttggaaaattgaaaataataatcaatattcatccgctgatgttagt 748

                                                                       
Query: 749 ttaatgttaacttggcaaaatagtattggtactcaatctgatcaagatggtggtcatttt 808
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 749 ttaatgttaacttggcaaaatagtattggtactcaatctgatcaagatggtggtcatttt 808

                                                   
Query: 809 aataaatattttacttttggtgataatgatcaacaatcac 848
           ||||||||||||||||||||||||||||||||||||||||
Sbjct: 809 aataaatattttacttttggtgataatgatcaacaatcac 848


 Score =  672 bits (339), Expect = 0.0
 Identities = 339/339 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   tatgcatgggcaagaagattagataacaattatccagagcataaacattttcaagtttca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tatgcatgggcaagaagattagataacaattatccagagcataaacattttcaagtttca 60

                                                                       
Query: 61  atgtggcaaggattgaaattggttggtatgggatatagaatgtggaaatatgtaaagaaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  atgtggcaaggattgaaattggttggtatgggatatagaatgtggaaatatgtaaagaaa 120

                                                                       
Query: 121 gaaacttcctctggaagagtaccaattatggatccattcaatctaccaaaacctggtcca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gaaacttcctctggaagagtaccaattatggatccattcaatctaccaaaacctggtcca 180

                                                                       
Query: 181 atcatgggtgtacccattggtggtattggtagtggtagtattaatcgtggttggaaaggt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atcatgggtgtacccattggtggtattggtagtggtagtattaatcgtggttggaaaggt 240

                                                                       
Query: 241 gatttcgttagatggaatcttaataatggtttagtttcgaatgaaatcgttgatgttgat 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gatttcgttagatggaatcttaataatggtttagtttcgaatgaaatcgttgatgttgat 300

                                                  
Query: 301 aaattttcagtttatattaattttgataattcaaatact 339
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 301 aaattttcagtttatattaattttgataattcaaatact 339


 Score =  492 bits (248), Expect = e-139
 Identities = 248/248 (100%)
 Strand = Plus / Plus

                                                                        
Query: 892  tataaaaggtattactatgattcataaaaaagaacaaaaatgtacattaccaaatggtaa 951
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 892  tataaaaggtattactatgattcataaaaaagaacaaaaatgtacattaccaaatggtaa 951

                                                                        
Query: 952  tacaaaggtatttaatgatccaatggaatattcaattgcagtaatggatgaaccagatgt 1011
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 952  tacaaaggtatttaatgatccaatggaatattcaattgcagtaatggatgaaccagatgt 1011

                                                                        
Query: 1012 tgaaattagtttttgtgaaagatttgaaactactagtagattggatgctgcaaatttatg 1071
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1012 tgaaattagtttttgtgaaagatttgaaactactagtagattggatgctgcaaatttatg 1071

                                                                        
Query: 1072 gtatacattcaataagaaaggtgaattggagaataataaagattctagaccatcagcacc 1131
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1072 gtatacattcaataagaaaggtgaattggagaataataaagattctagaccatcagcacc 1131

                    
Query: 1132 aaagaaac 1139
            ||||||||
Sbjct: 1132 aaagaaac 1139


 Score = 60.0 bits (30), Expect = 6e-09
 Identities = 30/30 (100%)
 Strand = Plus / Plus

                                         
Query: 367 aattcatcaacaccaccaccacaattttca 396
           ||||||||||||||||||||||||||||||
Sbjct: 367 aattcatcaacaccaccaccacaattttca 396


>Contig-U14871-1 (Contig-U14871-1Q) /CSM_Contig/Contig-U14871-1Q.Seq.d
          Length = 828

 Score = 40.1 bits (20), Expect = 0.005
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 201 tggtattggtagtggtagta 220
           ||||||||||||||||||||
Sbjct: 200 tggtattggtagtggtagta 219


>Contig-U14566-1 (Contig-U14566-1Q) /CSM_Contig/Contig-U14566-1Q.Seq.d
          Length = 501

 Score = 40.1 bits (20), Expect = 0.005
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                   
Query: 200 gtggtattggtagtggtagtatta 223
           |||||| |||||||||||||||||
Sbjct: 426 gtggtagtggtagtggtagtatta 449


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 20,554
Number of Sequences: 6905
Number of extensions: 20554
Number of successful extensions: 2763
Number of sequences better than 10.0: 347
length of query: 1139
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1123
effective length of database: 5,564,391
effective search space: 6248811093
effective search space used: 6248811093
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.12. 6
Homology vs DNA 
Query= Contig-U13162-1 (Contig-U13162-1Q) /CSM_Contig/Contig-U13162-1Q.Seq.d
         (1139 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421909) Dictyostelium discoideum cDNA clone:ddv44a09, 5' ...   672   0.0    3
(BJ378391) Dictyostelium discoideum cDNA clone:ddc31d24, 3' ...   381   0.0    4
(BJ426624) Dictyostelium discoideum cDNA clone:ddv59m14, 5' ...   412   0.0    2
(BJ371311) Dictyostelium discoideum cDNA clone:ddc57p11, 5' ...   331   5e-91  2
(BJ384607) Dictyostelium discoideum cDNA clone:ddc50g06, 3' ...   172   5e-69  2
(AC181787) Strongylocentrotus purpuratus clone R3-1030C18, W...    50   0.19   1
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...    38   0.27   13
(AX345334) Sequence 405 from Patent WO0200928.                     36   0.62   3
(AC087327) Trypanosoma brucei chromosome 4 clone RPCI93-3I12...    48   0.73   1
(AC130583) Rattus norvegicus clone CH230-66O6, *** SEQUENCIN...    48   0.73   1
(AC123124) Rattus norvegicus clone CH230-228F10, *** SEQUENC...    48   0.73   1
(AC179325) Strongylocentrotus purpuratus clone R3-57E10, WOR...    48   0.73   1
(AQ942389) Sheared DNA-54C18.TR Sheared DNA Trypanosoma bruc...    48   0.73   1
(CP000487) Campylobacter fetus subsp. fetus 82-40, complete ...    48   0.73   1
(AX346177) Sequence 1248 from Patent WO0200928.                    36   1.1    4
(AM428650) Vitis vinifera contig VV78X026452.9, whole genome...    32   1.9    2
(EJ924014) 1093018773692 Global-Ocean-Sampling_GS-30-02-01-1...    32   2.0    2
(EJ943994) 1093018903251 Global-Ocean-Sampling_GS-30-02-01-1...    32   2.0    2
(BG523931) 37-11 Stevia field grown leaf cDNA Stevia rebaudi...    36   2.2    2
(BG523317) 30-5 Stevia field grown leaf cDNA Stevia rebaudia...    36   2.5    2
(AC225082) Oryza officinalis clone OO__Ba0072B18, *** SEQUEN...    42   2.5    6
(AX346605) Sequence 1676 from Patent WO0200928.                    36   2.6    4
(CT027652) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    40   2.9    5
(DQ772518) Synthetic construct Yersinia pestis clone FLH0139...    46   2.9    1
(AC157606) Mus musculus chromosome 7, clone RP23-466J2, comp...    46   2.9    1
(AC217585) Populus trichocarpa clone POP021-H02, complete se...    46   2.9    1
(AC189484) Brassica rapa subsp. pekinensis clone KBrB083K19,...    46   2.9    1
(AC024799) Caenorhabditis elegans cosmid Y49C4A, complete se...    46   2.9    1
(AC157436) Oryctolagus cuniculus clone LB1-208G20, WORKING D...    46   2.9    1
(AC225324) Heliconius erato clone BBAM-15B24, WORKING DRAFT ...    46   2.9    1
(AC006788) Caenorhabditis elegans clone Y49C4, *** SEQUENCIN...    46   2.9    1
(BH466503) BOHAQ01TF BOHA Brassica oleracea genomic clone BO...    46   2.9    1
(EJ143703) 1092343675458 Global-Ocean-Sampling_GS-27-01-01-1...    46   2.9    1
(CZ738580) OC__Ba0076G24.f OC__Ba Oryza coarctata genomic cl...    46   2.9    1
(CZ694165) OC__Ba0006N15.r OC__Ba Oryza coarctata genomic cl...    46   2.9    1
(EG828435) EST_ssal_eve_40884 ssaleve thyroid Salmo salar cD...    46   2.9    1
(CP000950) Yersinia pseudotuberculosis YPIII, complete genome.     46   2.9    1
(CP000901) Yersinia pestis Angola, complete genome.                46   2.9    1
(CP000668) Yersinia pestis Pestoides F, complete genome.           46   2.9    1
(CP000308) Yersinia pestis Antiqua, complete genome.               46   2.9    1
(CP000305) Yersinia pestis Nepal516, complete genome.              46   2.9    1
(AL590842) Yersinia pestis CO92 complete genome.                   46   2.9    1
(AE017042) Yersinia pestis biovar Microtus str. 91001, compl...    46   2.9    1
(AE009952) Yersinia pestis KIM, complete genome.                   46   2.9    1
(CL032537) CH216-34K8_Sp6.1 CH216 Xenopus (Silurana) tropica...    38   3.7    2
(AC015771) Homo sapiens, clone RP11-2F20, complete sequence.       40   4.9    5
(AC015772) Homo sapiens chromosome 11 clone RP11-2F21 map 11...    40   4.9    5
(AP007663) Lotus japonicus genomic DNA, chromosome 1, clone:...    34   5.2    6
(AC009428) Homo sapiens chromosome , clone RP11-45M12, compl...    34   5.5    2
(AC068416) Homo sapiens chromosome 8 clone RP11-266C9 map 8.       34   5.5    2
(AP009790) Lotus japonicus genomic DNA, clone: LjT15P09, TM2...    34   5.6    2
(AP009787) Lotus japonicus genomic DNA, clone: LjT40K11, TM2...    34   5.7    2
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    34   6.1    11
(AC116960) Dictyostelium discoideum chromosome 2 map complem...    36   6.3    9
(DB771372) Apis mellifera head cDNA, RIKEN full-length enric...    32   6.7    3
(AM451575) Vitis vinifera contig VV78X027387.11, whole genom...    42   7.3    3
(ER585383) 1093015866743 Global-Ocean-Sampling_GS-36-01-01-2...    34   7.4    2
(DB758243) Apis mellifera head cDNA, RIKEN full-length enric...    32   7.6    3
(DB777100) Apis mellifera head cDNA, RIKEN full-length enric...    32   7.8    3
(DE018198) Branchiostoma floridae DNA, clone: CH302-029P11.R...    34   8.2    2
(AM631148) Entamoeba dispar GSS, clone dispar64f11.q1k.            38   9.2    2
(EL910767) INIT2_41_E09.g1_A006 G5 trophont cDNA (INIT2) Ich...    36   9.5    2
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    34   9.8    9

>(BJ421909) Dictyostelium discoideum cDNA clone:ddv44a09, 5' end,
           single read.
          Length = 586

 Score =  672 bits (339), Expect(3) = 0.0
 Identities = 339/339 (100%)
 Strand = Plus / Plus


Query: 1   tatgcatgggcaagaagattagataacaattatccagagcataaacattttcaagtttca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tatgcatgggcaagaagattagataacaattatccagagcataaacattttcaagtttca 60


Query: 61  atgtggcaaggattgaaattggttggtatgggatatagaatgtggaaatatgtaaagaaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  atgtggcaaggattgaaattggttggtatgggatatagaatgtggaaatatgtaaagaaa 120


Query: 121 gaaacttcctctggaagagtaccaattatggatccattcaatctaccaaaacctggtcca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gaaacttcctctggaagagtaccaattatggatccattcaatctaccaaaacctggtcca 180


Query: 181 atcatgggtgtacccattggtggtattggtagtggtagtattaatcgtggttggaaaggt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atcatgggtgtacccattggtggtattggtagtggtagtattaatcgtggttggaaaggt 240


Query: 241 gatttcgttagatggaatcttaataatggtttagtttcgaatgaaatcgttgatgttgat 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gatttcgttagatggaatcttaataatggtttagtttcgaatgaaatcgttgatgttgat 300


Query: 301 aaattttcagtttatattaattttgataattcaaatact 339
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 301 aaattttcagtttatattaattttgataattcaaatact 339

 Score =  224 bits (113), Expect(3) = 0.0
 Identities = 113/113 (100%)
 Strand = Plus / Plus


Query: 470 aaagtaaacaaaaagcagttgtattatatccaggtaaaccaaaatcaaatttaaatttag 529
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 470 aaagtaaacaaaaagcagttgtattatatccaggtaaaccaaaatcaaatttaaatttag 529


Query: 530 atgtttggaattggggattaaaaggtgataatagttgttattttggtttattt 582
           |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 530 atgtttggaattggggattaaaaggtgataatagttgttattttggtttattt 582

 Score = 42.1 bits (21), Expect(3) = 0.0
 Identities = 21/21 (100%)
 Strand = Plus / Plus


Query: 376 acaccaccaccacaattttca 396
           |||||||||||||||||||||
Sbjct: 376 acaccaccaccacaattttca 396

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,185,658,082
Number of extensions: 77854093
Number of successful extensions: 6244505
Number of sequences better than 10.0: 64
Length of query: 1139
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1115
Effective length of database: 97,308,875,965
Effective search space: 108499396700975
Effective search space used: 108499396700975
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 6
Homology vs Protein 
Query= Contig-U13162-1 (Contig-U13162-1Q) /CSM_Contig/Contig-U13162-1Q.Seq.d
         (1139 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK226969_1(AK226969|pid:none) Arabidopsis thaliana mRNA for hypo...   106   2e-32
BT002482_1(BT002482|pid:none) Arabidopsis thaliana putative prot...   106   2e-32
AK098954_1(AK098954|pid:none) Oryza sativa Japonica Group cDNA c...   103   7e-31
AK226971_1(AK226971|pid:none) Arabidopsis thaliana mRNA for hypo...   101   1e-30
AB028621_9(AB028621|pid:none) Arabidopsis thaliana genomic DNA, ...    94   3e-28
AK072300_1(AK072300|pid:none) Oryza sativa Japonica Group cDNA c...    96   4e-20
AC078948_16(AC078948|pid:none) Oryza sativa chromosome 10 BAC OS...    98   6e-19
AK027884_1(AK027884|pid:none) Homo sapiens cDNA FLJ14978 fis, cl...    90   6e-18
(Q9HCG7) RecName: Full=Non-lysosomal glucosylceramidase;        ...    90   2e-17
AB046825_1(AB046825|pid:none) Homo sapiens mRNA for KIAA1605 pro...    90   2e-17
AB384248_1(AB384248|pid:none) Synthetic construct DNA, clone: pF...    90   2e-17
AK297435_1(AK297435|pid:none) Homo sapiens cDNA FLJ55541 complet...    90   2e-17
BT004204_1(BT004204|pid:none) Arabidopsis thaliana clone RAFL16-...    92   3e-17
BT024218_1(BT024218|pid:none) Drosophila melanogaster RE08574 fu...    90   2e-16
(Q7KT91) RecName: Full=Non-lysosomal glucosylceramidase;        ...    90   2e-16
AK173213_1(AK173213|pid:none) Mus musculus mRNA for mKIAA1605 pr...    87   2e-16
(Q69ZF3) RecName: Full=Non-lysosomal glucosylceramidase;        ...    87   2e-16
BA000039_193(BA000039|pid:none) Thermosynechococcus elongatus BP...    74   1e-11
CP000110_900(CP000110|pid:none) Synechococcus sp. CC9605, comple...    73   2e-11
CP001037_1430(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...    73   2e-11
CP000360_1684(CP000360|pid:none) Acidobacteria bacterium Ellin34...    73   2e-11
CT971583_1695(CT971583|pid:none) Synechococcus WH7803 complete g...    73   2e-11
CP000100_852(CP000100|pid:none) Synechococcus elongatus PCC 7942...    68   5e-10
CP001344_4236(CP001344|pid:none) Cyanothece sp. PCC 7425, comple...    67   9e-10
CP000117_1094(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    67   9e-10
FN357479_3(FN357479|pid:none) Schistosoma mansoni genome sequenc...    59   2e-09
FN357479_4(FN357479|pid:none) Schistosoma mansoni genome sequenc...    59   2e-09
AI2157(AI2157) hypothetical protein alr2816 [imported] - Nostoc ...    66   3e-09
AM778953_149(AM778953|pid:none) Microcystis aeruginosa PCC 7806 ...    66   3e-09
CP000806_1607(CP000806|pid:none) Cyanothece sp. ATCC 51142 circu...    66   3e-09
BA000022_1086(BA000022|pid:none) Synechocystis sp. PCC 6803 DNA,...    64   8e-09
AM453212_4(AM453212|pid:none) Vitis vinifera contig VV78X031497....    64   1e-08
CP000097_1473(CP000097|pid:none) Synechococcus sp. CC9902, compl...    64   1e-08
BX548175_454(BX548175|pid:none) Prochlorococcus marinus MIT9313 ...    64   1e-08
AY811714_1(AY811714|pid:none) Schistosoma japonicum SJCHGC03819 ...    54   2e-08
AL132876_18(AL132876|pid:none) Caenorhabditis elegans YAC Y105E8...    63   2e-08
AL132876_17(AL132876|pid:none) Caenorhabditis elegans YAC Y105E8...    63   2e-08
AL132876_16(AL132876|pid:none) Caenorhabditis elegans YAC Y105E8...    63   2e-08
CP001291_271(CP001291|pid:none) Cyanothece sp. PCC 7424, complet...    63   2e-08
CT978603_1748(CT978603|pid:none) Synechococcus sp. RCC307 genomi...    51   7e-05
AC119671_4(AC119671|pid:none) Oryza sativa (japonica cultivar-gr...    34   3e-04
AF003385_1(AF003385|pid:none) Caenorhabditis elegans cosmid R08F...    46   0.003
BC088200_1(BC088200|pid:none) Rattus norvegicus glucosidase beta...    45   0.005
AL732626_3(AL732626|pid:none) Mouse DNA sequence from clone RP23...    42   0.031
AC010164_16(AC010164|pid:none) Arabidopsis thaliana chromosome I...    39   0.26

>AK226969_1(AK226969|pid:none) Arabidopsis thaliana mRNA for
            hypothetical protein, complete cds, clone: RAFL09-36-B11.
          Length = 957

 Score =  106 bits (264), Expect(2) = 2e-32
 Identities = 60/186 (32%), Positives = 95/186 (51%), Gaps = 4/186 (2%)
 Frame = +2

Query: 581  FPRAWTVYE-EPHPDIRLTCKQVSPVIPHNYQESSYPCGVYVWKIENNNQYSSADVSLML 757
            +PR+WT+YE EP P++R+ C+QVSP IPHNY+ESS+P  V+ + + N    ++ADV+L+
Sbjct: 182  YPRSWTMYEGEPDPELRIVCRQVSPFIPHNYKESSFPVSVFTFTLHNLGN-TTADVTLLF 240

Query: 758  TWQNSIGTQSDQDGGHFNKYFTFGDNDQQXXXXXXXXXXGNNKKNIKGITMIHKKEQKCT 937
            TW NS+G  S+  GGH+N   T  D                    ++G+ + HK
Sbjct: 241  TWANSVGGDSEFSGGHYNSKITMND-------------------GVQGVLLHHKTAN--G 279

Query: 938  LPNGNTKVFNDPMEYSIAVMDEPDVEISFCERFETTSRLD---AANLWYTFNKKGELENN 1108
            LP+         + Y+I+      V +S C  F  + + D   A ++W    + G  ++
Sbjct: 280  LPS---------LSYAISAQATDGVSVSACPFFIVSGKQDGITAKDMWQAVKENGSFDHL 330

Query: 1109 KDSRPS 1126
            K S  S
Sbjct: 331  KASEAS 336

 Score = 57.8 bits (138), Expect(2) = 2e-32
 Identities = 43/200 (21%), Positives = 72/200 (36%), Gaps = 5/200 (2%)
 Frame = +1

Query: 7   WARRLDNNYPEHKHFQVSMWQGLKLVGMGYRMWKYVKKETSSGRVPIMDPFNLXXXXXXX 186
           W R++D++    + F +S+ +  +L  +G R+W   ++E + GR+  +DPF+
Sbjct: 33  WQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGRLAFIDPFSKHSVTSSH 92

Query: 187 XXXXXXXXXXXXNRGWKGDFVRWNLNNGLVSNEIVDVDKFSVYXXXXXXXXXXXXXXXXX 366
                        R +KG+F RW L      +E V  ++FS +
Sbjct: 93  GVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAF----------------- 135

Query: 367 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSKQKAVVLYPGKPK-----SNLNLD 531
                                               K+ + VL P  PK     S   +
Sbjct: 136 ------------------------------VSRANGKKYSSVLCPRNPKLDKQDSESGIG 165

Query: 532 VWNWGLKGDNSCYFGLFSKS 591
            W+W LKGD S Y  L+ +S
Sbjct: 166 SWDWNLKGDKSTYHALYPRS 185

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,542,237,752
Number of extensions: 28021000
Number of successful extensions: 70232
Number of sequences better than 10.0: 45
Number of HSP's gapped: 70173
Number of HSP's successfully gapped: 77
Length of query: 379
Length of database: 1,051,180,864
Length adjustment: 130
Effective length of query: 249
Effective length of database: 630,428,194
Effective search space: 156976620306
Effective search space used: 156976620306
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.41  gvh: 0.26  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for Contig-U13162-1 is cyt
VS (DIR, S) 0
VH (FL, L) 2
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 3
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0