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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U12712-1
Contig ID Contig-U12712-1
Contig update 2002.12.18
Contig sequence 
>Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Contig-U12712-1Q.Seq.d
NNNNNNNNNNTTGGTATATTGGGGAGGAGGTAACCAAACAATTGAAATAT
AAAATATTTGAAACTATCTATGATAATCTCTTTCGAACCGTTATGAAATG
CACATCAGAGACAAAGTATAAGTATAATTTAGGCTGTTTCTGCTATTCTT
ACTTTAAGATCATTCAATCCGATCACCAAGTGACCTCTGCTAGATTCGTC
ACACAGAAACCACAAATTAAAGATGATCTCTACAAATATCTCTTACATTA
TCTTAGTCTTTCAAATGATCAACTTTCAAGAGCTTTATCGGATGTCCCAA
TCTCCGATTCCACAATTTGGTTAATCTATGCAAAAACATGTTGTCTATTG
AATCAAGAAGAGAAGGCTCAAATTTGGATCCAATATTTCATTAATGAAGC
TACTGATAGGGTAACAGCTGCTGCAATGATTCAATTGGATGAAGATATTG
CTCATTATCATTCAAAATCTTGGTATGTTGATATTATGAAATCAATTGAA
GAAGAGAAAAAGAAAAGAATTGAAGAAGATGATCGTTTAAATAAACAAAG
AAAGCAACAACAACAGCAACAACAAAGTAATTCATCATCACCACATATTG
TTGGAGGACATGTTCAAGATTTCAATCATCTCTTCTGTTGAAAGTCAAAG
TAACTTTTTCATTGAAATCATTCCATCTAAAATTTTAAATNATAAAGTTT
TATTATTATCTCATTATGCNAATTTCATTAGGGTTCTTTATGTCCATAAG
Gap no gap
Contig length 750
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 2443605
End point 2442863
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U12712
List of clone(s) 

est1=AHH287Z,1,741
Translated Amino Acid sequence 
XXXWYIGEEVTKQLKYKIFETIYDNLFRTVMKCTSETKYKYNLGCFCYSYFKIIQSDHQV
TSARFVTQKPQIKDDLYKYLLHYLSLSNDQLSRALSDVPISDSTIWLIYAKTCCLLNQEE
KAQIWIQYFINEATDRVTAAAMIQLDEDIAHYHSKSWYVDIMKSIEEEKKKRIEEDDRLN
KQRKQQQQQQQSNSSSPHIVGGHVQDFNHLFC*ksk*lfh*nhsi*nfkx*sfiiislcx
fh*gslcp*
Translated Amino Acid sequence (All Frames) 
Frame A:
xxxxgilgrr*pnn*nikylklsmiisfepl*nahqrqsisii*avsailtlrsfnpitk
*plldsshrnhklkmistnisyiilvfqminfqelyrmsqspipqfg*smqkhvvy*ikk
rrlkfgsnislmkllig*qllq*fnwmkilliiiqnlgmlil*nqlkkrkrkelkkmiv*
inkesnnnsnnkvihhhhilledmfkisiissvesqsnffieiipskilnxkvlllshya
nfirvlyvhk


Frame B:
xxxlvywgggnqtiei*ni*nyl**slsnryemhirdkv*v*frlfllfll*dhsirsps
dlc*irhtettn*r*slqisltls*sfk*stfksfigcpnlrfhnlvnlcknmlsiesrr
egsnldpifh**sy**gnsccndsig*rycslsfkilvc*yyein*rrekekn*rr*sfk
*tkkatttattk*fiittycwrtcsrfqsslllkvkvtfslksfhlkf*xikfyyylimx
islgffmsi


Frame C:
XXXWYIGEEVTKQLKYKIFETIYDNLFRTVMKCTSETKYKYNLGCFCYSYFKIIQSDHQV
TSARFVTQKPQIKDDLYKYLLHYLSLSNDQLSRALSDVPISDSTIWLIYAKTCCLLNQEE
KAQIWIQYFINEATDRVTAAAMIQLDEDIAHYHSKSWYVDIMKSIEEEKKKRIEEDDRLN
KQRKQQQQQQQSNSSSPHIVGGHVQDFNHLFC*ksk*lfh*nhsi*nfkx*sfiiislcx
fh*gslcp*
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U12712-1 (Contig-U12712-1Q)
/CSM_Contig/Contig-U12712-1Q.Seq.d
         (750 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Conti...   965  0.0
Contig-U11174-1 (Contig-U11174-1Q) /CSM_Contig/Conti...   965  0.0
Contig-U01567-1 (Contig-U01567-1Q) /CSM_Contig/Conti...    66  6e-11
Contig-U14511-1 (Contig-U14511-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U12248-1 (Contig-U12248-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U11357-1 (Contig-U11357-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U10914-1 (Contig-U10914-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U10906-1 (Contig-U10906-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U09903-1 (Contig-U09903-1Q) /CSM_Contig/Conti...    46  5e-05
Contig-U05843-1 (Contig-U05843-1Q) /CSM_Contig/Conti...    46  5e-05

>Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Contig-U12712-1Q.Seq.d
          Length = 750

 Score =  965 bits (487), Expect = 0.0
 Identities = 487/487 (100%)
 Strand = Plus / Plus

                                                                       
Query: 11  ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 11  ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 70

                                                                       
Query: 71  tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71  tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 130

                                                                       
Query: 131 aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 190
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 131 aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 190

                                                                       
Query: 191 tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 250
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 191 tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 250

                                                                       
Query: 251 tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 310
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 251 tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 310

                                                                       
Query: 311 cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 370
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 311 cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 370

                                                                       
Query: 371 aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 430
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 371 aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 430

                                                                       
Query: 431 tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 490
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 431 tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 490

                  
Query: 491 atcaatt 497
           |||||||
Sbjct: 491 atcaatt 497


 Score =  426 bits (215), Expect = e-119
 Identities = 221/221 (100%)
 Strand = Plus / Plus

                                                                       
Query: 530 tgatcgtttaaataaacaaagaaagcaacaacaacagcaacaacaaagtaattcatcatc 589
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 530 tgatcgtttaaataaacaaagaaagcaacaacaacagcaacaacaaagtaattcatcatc 589

                                                                       
Query: 590 accacatattgttggaggacatgttcaagatttcaatcatctcttctgttgaaagtcaaa 649
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 590 accacatattgttggaggacatgttcaagatttcaatcatctcttctgttgaaagtcaaa 649

                                                                       
Query: 650 gtaactttttcattgaaatcattccatctaaaattttaaatnataaagttttattattat 709
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 650 gtaactttttcattgaaatcattccatctaaaattttaaatnataaagttttattattat 709

                                                    
Query: 710 ctcattatgcnaatttcattagggttctttatgtccataag 750
           |||||||||||||||||||||||||||||||||||||||||
Sbjct: 710 ctcattatgcnaatttcattagggttctttatgtccataag 750


>Contig-U11174-1 (Contig-U11174-1Q) /CSM_Contig/Contig-U11174-1Q.Seq.d
          Length = 2587

 Score =  965 bits (487), Expect = 0.0
 Identities = 487/487 (100%)
 Strand = Plus / Plus

                                                                        
Query: 11   ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 70
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1387 ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 1446

                                                                        
Query: 71   tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 130
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1447 tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 1506

                                                                        
Query: 131  aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 190
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1507 aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 1566

                                                                        
Query: 191  tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 250
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1567 tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 1626

                                                                        
Query: 251  tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 310
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1627 tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 1686

                                                                        
Query: 311  cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 370
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1687 cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 1746

                                                                        
Query: 371  aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 430
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1747 aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 1806

                                                                        
Query: 431  tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 490
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1807 tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 1866

                   
Query: 491  atcaatt 497
            |||||||
Sbjct: 1867 atcaatt 1873


 Score =  228 bits (115), Expect = 7e-60
 Identities = 129/133 (96%), Gaps = 1/133 (0%)
 Strand = Plus / Plus

                                                                        
Query: 616  aagatttcaatcatctcttctgttgaaagtcaaagtaactttttcattgaaatcattcca 675
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2444 aagatttcaatcatctcttctgttgaaagtcaaagtaactttttcattgaaatcattcca 2503

                                                                        
Query: 676  tctaaaattttaaatnataaagttttattattatctcattatgc-naatttcattagggt 734
            ||||||||||||||| ||||||||||||||||||||||||||||  |||||||||| |||
Sbjct: 2504 tctaaaattttaaatgataaagttttattattatctcattatgctgaatttcattatggt 2563

                         
Query: 735  tctttatgtccat 747
            |||||||||||||
Sbjct: 2564 tctttatgtccat 2576


 Score =  180 bits (91), Expect = 1e-45
 Identities = 91/91 (100%)
 Strand = Plus / Plus

                                                                        
Query: 530  tgatcgtttaaataaacaaagaaagcaacaacaacagcaacaacaaagtaattcatcatc 589
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1906 tgatcgtttaaataaacaaagaaagcaacaacaacagcaacaacaaagtaattcatcatc 1965

                                           
Query: 590  accacatattgttggaggacatgttcaagat 620
            |||||||||||||||||||||||||||||||
Sbjct: 1966 accacatattgttggaggacatgttcaagat 1996


 Score = 42.1 bits (21), Expect = 9e-04
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 555 caacaacaacagcaacaacaa 575
           |||||||||||||||||||||
Sbjct: 349 caacaacaacagcaacaacaa 369


 Score = 38.2 bits (19), Expect = 0.013
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 557 acaacaacagcaacaacaa 575
           |||||||||||||||||||
Sbjct: 684 acaacaacagcaacaacaa 702


 Score = 30.2 bits (15), Expect = 3.2
 Identities = 21/23 (91%)
 Strand = Plus / Plus

                                  
Query: 553 agcaacaacaacagcaacaacaa 575
           ||||||||||||| |||| ||||
Sbjct: 359 agcaacaacaacaacaactacaa 381


>Contig-U01567-1 (Contig-U01567-1Q) /CSM_Contig/Contig-U01567-1Q.Seq.d
          Length = 446

 Score = 65.9 bits (33), Expect = 6e-11
 Identities = 65/77 (84%)
 Strand = Plus / Plus

                                                                       
Query: 642 aagtcaaagtaactttttcattgaaatcattccatctaaaattttaaatnataaagtttt 701
           ||||||||| || |||||||| | ||||||||||| ||||| ||||||   ||||| |||
Sbjct: 133 aagtcaaagnaaatttttcatnggaatcattccatttaaaagtttaaagggtaaaggttt 192

                            
Query: 702 attattatctcattatg 718
           |||||||| ||| ||||
Sbjct: 193 attattatttcantatg 209


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 17,811
Number of Sequences: 6905
Number of extensions: 17811
Number of successful extensions: 4697
Number of sequences better than 10.0: 709
length of query: 750
length of database: 5,674,871
effective HSP length: 16
effective length of query: 734
effective length of database: 5,564,391
effective search space: 4084262994
effective search space used: 4084262994
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.12. 2
Homology vs DNA 
Query= Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Contig-U12712-1Q.Seq.d
         (750 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ352179) Dictyostelium discoideum cDNA clone:dda45m22, 3' ...   965   0.0    2
(AU061965) Dictyostelium discoideum slug cDNA, clone SLG802.      940   0.0    1
(BJ363268) Dictyostelium discoideum cDNA clone:ddc25h24, 5' ...   856   0.0    1
(BJ411095) Dictyostelium discoideum cDNA clone:ddv3a03, 5' e...   595   e-166  1
(BJ382274) Dictyostelium discoideum cDNA clone:ddc44o04, 3' ...   224   e-149  4
(BJ347871) Dictyostelium discoideum cDNA clone:dda31o10, 3' ...   242   e-148  3
(BJ354377) Dictyostelium discoideum cDNA clone:dda53m09, 3' ...   236   e-148  3
(BJ354362) Dictyostelium discoideum cDNA clone:dda53j11, 3' ...   220   e-147  4
(BJ445311) Dictyostelium discoideum cDNA clone:ddv59h06, 3' ...   220   e-147  4
(BJ348911) Dictyostelium discoideum cDNA clone:dda34c22, 3' ...   234   e-147  3
(BJ351127) Dictyostelium discoideum cDNA clone:dda42k02, 3' ...   232   e-146  3
(BJ355205) Dictyostelium discoideum cDNA clone:dda56f05, 3' ...   228   e-145  3
(BJ347768) Dictyostelium discoideum cDNA clone:dda31i06, 3' ...   222   e-144  3
(BJ354019) Dictyostelium discoideum cDNA clone:dda52b12, 3' ...   218   e-142  3
(BJ378645) Dictyostelium discoideum cDNA clone:ddc32k11, 3' ...   222   e-141  3
(BJ383090) Dictyostelium discoideum cDNA clone:ddc46h19, 3' ...   218   e-136  3
(BJ342503) Dictyostelium discoideum cDNA clone:dda14g11, 3' ...   198   e-133  5
(BJ352067) Dictyostelium discoideum cDNA clone:dda45d17, 3' ...   220   e-132  4
(BJ404399) Dictyostelium discoideum cDNA clone:dds28l09, 3' ...   220   e-131  4
(BJ378443) Dictyostelium discoideum cDNA clone:ddc31a04, 3' ...   220   e-131  4
(BJ384074) Dictyostelium discoideum cDNA clone:ddc48l06, 3' ...   220   e-130  4
(BJ386127) Dictyostelium discoideum cDNA clone:ddc57a11, 3' ...   216   e-127  3
(BJ379126) Dictyostelium discoideum cDNA clone:ddc33i24, 3' ...   220   e-126  3
(BJ378522) Dictyostelium discoideum cDNA clone:ddc32b05, 3' ...   218   e-126  3
(BJ437063) Dictyostelium discoideum cDNA clone:ddv33g02, 3' ...   214   e-125  3
(BJ404046) Dictyostelium discoideum cDNA clone:dds27j06, 3' ...   212   e-123  4
(BJ356489) Dictyostelium discoideum cDNA clone:dda60a13, 3' ...   206   e-122  3
(BJ349305) Dictyostelium discoideum cDNA clone:dda36g06, 3' ...   214   e-121  3
(BJ374812) Dictyostelium discoideum cDNA clone:ddc16k08, 3' ...   216   e-120  3
(BJ441620) Dictyostelium discoideum cDNA clone:ddv47e09, 3' ...   165   e-120  4
(BJ383418) Dictyostelium discoideum cDNA clone:ddc52o23, 3' ...   188   e-119  5
(BJ383742) Dictyostelium discoideum cDNA clone:ddc47h02, 3' ...   216   e-119  4
(BJ379670) Dictyostelium discoideum cDNA clone:ddc35i17, 3' ...   216   e-119  4
(BJ350603) Dictyostelium discoideum cDNA clone:dda40n09, 3' ...   230   e-118  4
(BJ385341) Dictyostelium discoideum cDNA clone:ddc54k15, 3' ...   180   e-115  3
(BJ345737) Dictyostelium discoideum cDNA clone:dda28l17, 3' ...   178   e-114  4
(BJ348681) Dictyostelium discoideum cDNA clone:dda34b03, 3' ...   220   e-113  3
(BJ383349) Dictyostelium discoideum cDNA clone:ddc52p17, 3' ...   176   e-111  3
(BJ354168) Dictyostelium discoideum cDNA clone:dda52c20, 3' ...   256   e-109  4
(BJ384642) Dictyostelium discoideum cDNA clone:ddc50p01, 3' ...   170   e-107  5
(BJ401044) Dictyostelium discoideum cDNA clone:dds15o23, 3' ...   165   e-104  3
(BJ343482) Dictyostelium discoideum cDNA clone:dda16a07, 3' ...   153   e-101  3
(BJ379247) Dictyostelium discoideum cDNA clone:ddc34a09, 3' ...   218   2e-99  3
(BJ445407) Dictyostelium discoideum cDNA clone:ddv59j10, 3' ...   200   1e-97  3
(BJ385016) Dictyostelium discoideum cDNA clone:ddc51d14, 3' ...   131   6e-96  6
(BJ352352) Dictyostelium discoideum cDNA clone:dda46a14, 3' ...   220   7e-96  4
(BJ386244) Dictyostelium discoideum cDNA clone:ddc57h18, 3' ...   192   3e-95  3
(BJ383165) Dictyostelium discoideum cDNA clone:ddc52g05, 3' ...   174   3e-95  3
(BJ383881) Dictyostelium discoideum cDNA clone:ddc47e18, 3' ...   208   4e-94  3
(BJ378745) Dictyostelium discoideum cDNA clone:ddc32o15, 3' ...   218   2e-93  3
(BJ379932) Dictyostelium discoideum cDNA clone:ddc36a14, 3' ...   218   2e-92  3
(BJ379234) Dictyostelium discoideum cDNA clone:ddc34o02, 3' ...   220   9e-92  3
(BJ348655) Dictyostelium discoideum cDNA clone:dda33m19, 3' ...   218   5e-90  3
(BJ429295) Dictyostelium discoideum cDNA clone:ddv3a03, 3' e...   135   9e-89  4
(BJ404065) Dictyostelium discoideum cDNA clone:dds27o03, 3' ...   222   2e-85  2
(BJ440609) Dictyostelium discoideum cDNA clone:ddv44p02, 3' ...   220   1e-83  3
(BJ382616) Dictyostelium discoideum cDNA clone:ddc45g12, 3' ...   220   5e-83  3
(BJ353846) Dictyostelium discoideum cDNA clone:dda51j17, 3' ...   220   5e-83  3
(BJ380796) Dictyostelium discoideum cDNA clone:ddc39i07, 3' ...   212   2e-82  2
(BJ382434) Dictyostelium discoideum cDNA clone:ddc44a22, 3' ...   135   2e-81  5
(BJ378740) Dictyostelium discoideum cDNA clone:ddc32n16, 3' ...   208   6e-81  2
(BJ445040) Dictyostelium discoideum cDNA clone:ddv58b07, 3' ...   220   1e-80  3
(BJ379735) Dictyostelium discoideum cDNA clone:ddc35g21, 3' ...   220   8e-77  2
(BJ347811) Dictyostelium discoideum cDNA clone:dda31b12, 3' ...   218   3e-76  2
(BJ379570) Dictyostelium discoideum cDNA clone:ddc35e09, 3' ...   220   8e-74  2
(BJ439339) Dictyostelium discoideum cDNA clone:ddv40k03, 3' ...   206   2e-71  2
(BJ355052) Dictyostelium discoideum cDNA clone:dda55i13, 3' ...   131   1e-70  4
(BJ441105) Dictyostelium discoideum cDNA clone:ddv45n15, 3' ...   196   1e-69  2
(BJ363375) Dictyostelium discoideum cDNA clone:ddc26g12, 5' ...   276   1e-69  1
(BJ383583) Dictyostelium discoideum cDNA clone:ddc53d15, 3' ...   131   2e-69  3
(BJ385621) Dictyostelium discoideum cDNA clone:ddc55f18, 3' ...   161   1e-66  2
(BJ383654) Dictyostelium discoideum cDNA clone:ddc53d20, 3' ...   176   1e-66  2
(BJ357232) Dictyostelium discoideum cDNA clone:dda62j19, 3' ...   149   2e-65  4
(BJ381107) Dictyostelium discoideum cDNA clone:ddc40k08, 3' ...   188   6e-65  2
(BJ383580) Dictyostelium discoideum cDNA clone:ddc53c17, 3' ...   131   1e-61  3
(BJ381810) Dictyostelium discoideum cDNA clone:ddc42a19, 3' ...   220   3e-58  2
(BJ441087) Dictyostelium discoideum cDNA clone:ddv45k13, 3' ...   220   3e-58  2
(BJ381356) Dictyostelium discoideum cDNA clone:ddc41c11, 3' ...   220   3e-58  2
(BJ382051) Dictyostelium discoideum cDNA clone:ddc43a23, 3' ...   220   3e-58  2
(BJ381913) Dictyostelium discoideum cDNA clone:ddc43g10, 3' ...   220   3e-58  2
(BJ381781) Dictyostelium discoideum cDNA clone:ddc42k13, 3' ...   220   1e-57  2
(BJ377771) Dictyostelium discoideum cDNA clone:ddc25h24, 3' ...   149   6e-55  4
(AU039945) Dictyostelium discoideum slug cDNA, clone SLG802.      200   1e-54  2
(AU267264) Dictyostelium discoideum vegetative cDNA clone:VS...   200   1e-54  2
(BJ344767) Dictyostelium discoideum cDNA clone:dda20n08, 3' ...   131   6e-53  5
(BJ345493) Dictyostelium discoideum cDNA clone:dda23i24, 3' ...   125   3e-52  5
(BJ381604) Dictyostelium discoideum cDNA clone:ddc42e05, 3' ...   198   2e-46  1
(BJ445420) Dictyostelium discoideum cDNA clone:ddv59l12, 3' ...   182   1e-41  1
(AU037427) Dictyostelium discoideum slug cDNA, clone SSD625.      170   5e-38  1
(BJ377896) Dictyostelium discoideum cDNA clone:ddc26g12, 3' ...    94   3e-34  2
(BJ380261) Dictyostelium discoideum cDNA clone:ddc37g16, 3' ...    90   2e-32  2
(BJ380357) Dictyostelium discoideum cDNA clone:ddc37l23, 3' ...    92   1e-30  2
(BJ384387) Dictyostelium discoideum cDNA clone:ddc49d07, 3' ...   107   4e-30  3
(BJ349751) Dictyostelium discoideum cDNA clone:dda37d15, 3' ...   111   4e-20  1
(BJ380147) Dictyostelium discoideum cDNA clone:ddc37n02, 3' ...   105   2e-18  1
(BJ381982) Dictyostelium discoideum cDNA clone:ddc43d14, 3' ...    72   3e-08  1
(AU037267) Dictyostelium discoideum slug cDNA, clone SSB690.       66   2e-06  1
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    44   0.001  10
(FF509889) G709P523RC1.T0 Acorn worm normalized neurula pExp...    42   0.007  2
(FF485814) G708P5111RB5.T0 Acorn worm normalized gastrula pE...    42   0.007  2

>(BJ352179) Dictyostelium discoideum cDNA clone:dda45m22, 3' end,
           single read.
          Length = 741

 Score =  965 bits (487), Expect(2) = 0.0
 Identities = 487/487 (100%)
 Strand = Plus / Minus


Query: 11  ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 741 ttggtatattggggaggaggtaaccaaacaattgaaatataaaatatttgaaactatcta 682


Query: 71  tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 681 tgataatctctttcgaaccgttatgaaatgcacatcagagacaaagtataagtataattt 622


Query: 131 aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 190
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 621 aggctgtttctgctattcttactttaagatcattcaatccgatcaccaagtgacctctgc 562


Query: 191 tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 250
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 561 tagattcgtcacacagaaaccacaaattaaagatgatctctacaaatatctcttacatta 502


Query: 251 tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 310
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 501 tcttagtctttcaaatgatcaactttcaagagctttatcggatgtcccaatctccgattc 442


Query: 311 cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 370
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 441 cacaatttggttaatctatgcaaaaacatgttgtctattgaatcaagaagagaaggctca 382


Query: 371 aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 430
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 aatttggatccaatatttcattaatgaagctactgatagggtaacagctgctgcaatgat 322


Query: 431 tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 490
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 tcaattggatgaagatattgctcattatcattcaaaatcttggtatgttgatattatgaa 262


Query: 491 atcaatt 497
           |||||||
Sbjct: 261 atcaatt 255

 Score =  377 bits (190), Expect(2) = 0.0
 Identities = 196/196 (100%)
 Strand = Plus / Minus


Query: 555 caacaacaacagcaacaacaaagtaattcatcatcaccacatattgttggaggacatgtt 614
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 197 caacaacaacagcaacaacaaagtaattcatcatcaccacatattgttggaggacatgtt 138


Query: 615 caagatttcaatcatctcttctgttgaaagtcaaagtaactttttcattgaaatcattcc 674
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 137 caagatttcaatcatctcttctgttgaaagtcaaagtaactttttcattgaaatcattcc 78


Query: 675 atctaaaattttaaatnataaagttttattattatctcattatgcnaatttcattagggt 734
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 77  atctaaaattttaaatnataaagttttattattatctcattatgcnaatttcattagggt 18


Query: 735 tctttatgtccataag 750
           ||||||||||||||||
Sbjct: 17  tctttatgtccataag 2

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 837,753,075
Number of extensions: 54938295
Number of successful extensions: 5294505
Number of sequences better than 10.0: 2250
Length of query: 750
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 726
Effective length of database: 97,308,875,965
Effective search space: 70646243950590
Effective search space used: 70646243950590
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 4
Homology vs Protein 
Query= Contig-U12712-1 (Contig-U12712-1Q) /CSM_Contig/Contig-U12712-1Q.Seq.d
         (750 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AL662987_21(AL662987|pid:none) Oryza sativa genomic DNA, chromos...    35   3.3
AP008210_1680(AP008210|pid:none) Oryza sativa (japonica cultivar...    35   3.3

>AL662987_21(AL662987|pid:none) Oryza sativa genomic DNA, chromosome
           4, BAC clone: OSJNBa0088A01, complete sequence.
           &FB850083_1(FB850083|pid:none)
           &FB905197_1(FB905197|pid:none)
           &FB914454_1(FB914454|pid:none)
           &FB919072_1(FB919072|pid:none)
          Length = 677

 Score = 34.7 bits (78), Expect = 3.3
 Identities = 18/47 (38%), Positives = 27/47 (57%)
 Frame = +3

Query: 345 LLNQEEKAQIWIQYFINEATDRVTAAAMIQLDEDIAHYHSKSWYVDI 485
           +L+     Q+ I Y    +T++V  A +IQ D   AHY SK+W VD+
Sbjct: 185 ILSSGADVQVRITY---NSTEQVLVAILIQFDTTGAHYGSKAWSVDL 228

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 993,643,710
Number of extensions: 15610737
Number of successful extensions: 28268
Number of sequences better than 10.0: 2
Number of HSP's gapped: 28263
Number of HSP's successfully gapped: 2
Length of query: 250
Length of database: 1,051,180,864
Length adjustment: 125
Effective length of query: 125
Effective length of database: 646,610,989
Effective search space: 80826373625
Effective search space used: 80826373625
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U12712-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 1
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0