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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U12467-1
Contig ID Contig-U12467-1
Contig update 2002.12.18
Contig sequence 
>Contig-U12467-1 (Contig-U12467-1Q) /CSM_Contig/Contig-U12467-1Q.Seq.d
CCAATTTCTTATTCAACTGTTAATTTATCTCAAATCCTTCAACAAGGTGA
GTTTACATCAGATTTAGATATGACACCAATTATATCCCATACAAACTATG
GTGGTAATGTTAATAGTAGTGTTGGTAGTAATAATGATTTTATAAATAGT
GGTAATAGCTCACAACAGTATCAACAACAACAACAACAACAGCAGCAACA
ACAACAACAGCAACAACAGCAACCAATACCAACTACTACTCTAAATATAA
CTATTTGTAGTGTTCCAAAAGAAATTGAAGATTTAGAAAATGCACAAGAA
TATCAATTGAATGATATGGGATTAAACTCTGATGATGAGAATTTATTCAT
TGATTCAGATTCAAATGATGAATATGATGAAGAAGTTATATCATACCAAG
ATGTACCTTATCATTTCAATCCAATCACTAGACCTTCAAACTATGGTAAT
TCTCCAACTTTATTAAGTTCATCTCCTTCAACATTTCCTGATAATAGTCA
TCATCATCATCATCATCATCATGAAACCGATCAAGAAATTGAAAATCATC
ACCATCACAATCACAGTCACAATCACAATCATCATAACCATCATCATAAC
CAACAATTTATAAATNNNNNNNNN----------NNNNNNNNNNAGAAAA
ACATGTTANCAAGATTTGGTAGTAGTAGTAGTAGTAGTGGAAATAGTAGC
GTTTTAAGTGGAATCAGTAATAATATCAATAGTATTAGTAGTGGAATTAA
TAGTAACATTGGAAATAATAATCAAAATAGTGGTAGTAATAGTAGTGGTG
GTGGTAGTAGTAATAGTATTAGTAGTTCAAAAAAGAAAGATGAAAATAAA
AATAGTTCCTCAATATCATCTTCAAATACATTAAATAGTGGTGATAGTAA
AAATAGTAAAGATGAAAAACAATCTTCTTCTAAAGATTCACAGAAACAAG
ACCATGATAAAAAAGAAAATGATTCAAGTTTAATTTTGTCAATTACAAAA
TTAATGTGTGGCCCAGTGGCTTCAACTTCTTCATCTTCATCCTCTTCTTC
AAACACTGAATCAATATACTTTAAGGTTTTAATTGATGGTGTAGAGTTTA
ACAATGTTAAATTCTTATCAATATCTCCACAATGGGGTACTCATGTTAAA
TATTTCCCTGTTCAATCATTTGTTGAACAAAAACAATCTGACAACCAAGA
AAATCACCATAATAATCATAATCATAATCATCACCATCATACAAANNATA
TTTATTCAGNATTAATAATAAATTTTTTT
Gap gap included
Contig length 1269
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 6212109
End point 6213360
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 2
Link to clone list U12467
List of clone(s) 

est1=VHG427F,1,615
est2=VHG427Z,616,1251
Translated Amino Acid sequence 
PISYSTVNLSQILQQGEFTSDLDMTPIISHTNYGGNVNSSVGSNNDFINSGNSSQQYQQQ
QQQQQQQQQQQQQQPIPTTTLNITICSVPKEIEDLENAQEYQLNDMGLNSDDENLFIDSD
SNDEYDEEVISYQDVPYHFNPITRPSNYGNSPTLLSSSPSTFPDNSHHHHHHHHETDQEI
ENHHHHNHSHNHNHHNHHHNQQFINXXX---

---XXXXKNMLXRFGSSSSSSGNSSVLSGISNNINSISSGINSNIGNNNQNSGSNSSGGG
SSNSISSSKKKDENKNSSSISSSNTLNSGDSKNSKDEKQSSSKDSQKQDHDKKENDSSLI
LSITKLMCGPVASTSSSSSSSSNTESIYFKVLIDGVEFNNVKFLSISPQWGTHVKYFPVQ
SFVEQKQSDNQENHHNNHNHNHHHHTXXIYSXLIINFF
Translated Amino Acid sequence (All Frames) 
Frame A:
PISYSTVNLSQILQQGEFTSDLDMTPIISHTNYGGNVNSSVGSNNDFINSGNSSQQYQQQ
QQQQQQQQQQQQQQPIPTTTLNITICSVPKEIEDLENAQEYQLNDMGLNSDDENLFIDSD
SNDEYDEEVISYQDVPYHFNPITRPSNYGNSPTLLSSSPSTFPDNSHHHHHHHHETDQEI
ENHHHHNHSHNHNHHNHHHNQQFINXXX---

---XXXXKNMLXRFGSSSSSSGNSSVLSGISNNINSISSGINSNIGNNNQNSGSNSSGGG
SSNSISSSKKKDENKNSSSISSSNTLNSGDSKNSKDEKQSSSKDSQKQDHDKKENDSSLI
LSITKLMCGPVASTSSSSSSSSNTESIYFKVLIDGVEFNNVKFLSISPQWGTHVKYFPVQ
SFVEQKQSDNQENHHNNHNHNHHHHTXXIYSXLIINFF

Frame B:
qfliqlliylksfnkvslhqi*i*hqlypiqtmvvmlivvlvvimil*ivviahnsinnn
nnnssnnnnsnnsnqyqlll*i*lfvvfqkklki*kmhknin*miwd*tlmmriysliqi
qmmnmmkklyhtkmyliisiqsldlqtmvilqly*vhllqhfliiviiiiiiimkpikkl
kiitititvtitiiitiiitnnl*xxx---

---xxxrktcxqdlvvvvvvveivaf*vesviisivlvvelivtleiiikivvvivvvvv
vvivlvvqkrkmkikivpqyhlqih*ivvivkivkmknnlllkihrnktmikkkmiqv*f
cqlqn*cvaqwlqllhlhpllqtlnqytlrf*lmv*sltmlnsyqylhngvlmlnislfn
hllnknnlttkkitiiiiiiiitiiqxifiqx***if

Frame C:
nflfnc*fisnpstr*vyirfrydtnyipyklww*c***cw****fyk*w**lttvsttt
tttaattttattatntnyyskynyl*cskrn*rfrkctrisie*ygikl***efih*frf
k**i**rsyiiprctlsfqsnh*tfklw*fsnfikfisfnis***ssssssss*nrsrn*
ksspsqsqsqsqss*pss*ptiykxxx---

---xxxekhvxkiw******wk**rfkwnq**yq*y**wn***hwk**sk*w****www*
***y**fkker*k*k*flniifkyik*w***k**r*ktiff*rftetrp**krk*fkfnf
vnykinvwpsgfnffifilffkh*inil*gfn*wcrv*qc*ilinistmgysc*ifpcsi
ic*tkti*qprksp**s*s*sspsykxylfxinnkff
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U12467-1 (Contig-U12467-1Q)
/CSM_Contig/Contig-U12467-1Q.Seq.d
         (1279 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U12467-1 (Contig-U12467-1Q) /CSM_Contig/Conti...   815  0.0
Contig-U13938-1 (Contig-U13938-1Q) /CSM_Contig/Conti...    48  2e-05
Contig-U12803-1 (Contig-U12803-1Q) /CSM_Contig/Conti...    46  9e-05
Contig-U10073-1 (Contig-U10073-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U04147-1 (Contig-U04147-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U13053-1 (Contig-U13053-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U12316-1 (Contig-U12316-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U11391-1 (Contig-U11391-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U06780-1 (Contig-U06780-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U02849-1 (Contig-U02849-1Q) /CSM_Contig/Conti...    42  0.001

>Contig-U12467-1 (Contig-U12467-1Q) /CSM_Contig/Contig-U12467-1Q.Seq.d
          Length = 1279

 Score =  815 bits (411), Expect = 0.0
 Identities = 420/420 (100%)
 Strand = Plus / Plus

                                                                        
Query: 853  tagttcctcaatatcatcttcaaatacattaaatagtggtgatagtaaaaatagtaaaga 912
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 853  tagttcctcaatatcatcttcaaatacattaaatagtggtgatagtaaaaatagtaaaga 912

                                                                        
Query: 913  tgaaaaacaatcttcttctaaagattcacagaaacaagaccatgataaaaaagaaaatga 972
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 913  tgaaaaacaatcttcttctaaagattcacagaaacaagaccatgataaaaaagaaaatga 972

                                                                        
Query: 973  ttcaagtttaattttgtcaattacaaaattaatgtgtggcccagtggcttcaacttcttc 1032
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 973  ttcaagtttaattttgtcaattacaaaattaatgtgtggcccagtggcttcaacttcttc 1032

                                                                        
Query: 1033 atcttcatcctcttcttcaaacactgaatcaatatactttaaggttttaattgatggtgt 1092
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1033 atcttcatcctcttcttcaaacactgaatcaatatactttaaggttttaattgatggtgt 1092

                                                                        
Query: 1093 agagtttaacaatgttaaattcttatcaatatctccacaatggggtactcatgttaaata 1152
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1093 agagtttaacaatgttaaattcttatcaatatctccacaatggggtactcatgttaaata 1152

                                                                        
Query: 1153 tttccctgttcaatcatttgttgaacaaaaacaatctgacaaccaagaaaatcaccataa 1212
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1153 tttccctgttcaatcatttgttgaacaaaaacaatctgacaaccaagaaaatcaccataa 1212

                                                                        
Query: 1213 taatcataatcataatcatcaccatcatacaaannatatttattcagnattaataataaa 1272
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1213 taatcataatcataatcatcaccatcatacaaannatatttattcagnattaataataaa 1272


 Score =  543 bits (274), Expect = e-154
 Identities = 274/274 (100%)
 Strand = Plus / Plus

                                                                       
Query: 224 caataccaactactactctaaatataactatttgtagtgttccaaaagaaattgaagatt 283
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 224 caataccaactactactctaaatataactatttgtagtgttccaaaagaaattgaagatt 283

                                                                       
Query: 284 tagaaaatgcacaagaatatcaattgaatgatatgggattaaactctgatgatgagaatt 343
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 284 tagaaaatgcacaagaatatcaattgaatgatatgggattaaactctgatgatgagaatt 343

                                                                       
Query: 344 tattcattgattcagattcaaatgatgaatatgatgaagaagttatatcataccaagatg 403
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 344 tattcattgattcagattcaaatgatgaatatgatgaagaagttatatcataccaagatg 403

                                                                       
Query: 404 taccttatcatttcaatccaatcactagaccttcaaactatggtaattctccaactttat 463
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 404 taccttatcatttcaatccaatcactagaccttcaaactatggtaattctccaactttat 463

                                             
Query: 464 taagttcatctccttcaacatttcctgataatag 497
           ||||||||||||||||||||||||||||||||||
Sbjct: 464 taagttcatctccttcaacatttcctgataatag 497


 Score =  319 bits (161), Expect = 4e-87
 Identities = 161/161 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   ccaatttcttattcaactgttaatttatctcaaatccttcaacaaggtgagtttacatca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   ccaatttcttattcaactgttaatttatctcaaatccttcaacaaggtgagtttacatca 60

                                                                       
Query: 61  gatttagatatgacaccaattatatcccatacaaactatggtggtaatgttaatagtagt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  gatttagatatgacaccaattatatcccatacaaactatggtggtaatgttaatagtagt 120

                                                    
Query: 121 gttggtagtaataatgattttataaatagtggtaatagctc 161
           |||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gttggtagtaataatgattttataaatagtggtaatagctc 161


 Score =  188 bits (95), Expect = 1e-47
 Identities = 95/95 (100%)
 Strand = Plus / Plus

                                                                       
Query: 689 ggaaatagtagcgttttaagtggaatcagtaataatatcaatagtattagtagtggaatt 748
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 689 ggaaatagtagcgttttaagtggaatcagtaataatatcaatagtattagtagtggaatt 748

                                              
Query: 749 aatagtaacattggaaataataatcaaaatagtgg 783
           |||||||||||||||||||||||||||||||||||
Sbjct: 749 aatagtaacattggaaataataatcaaaatagtgg 783


 Score = 42.1 bits (21), Expect = 0.001
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                   
Query: 645 agaaaaacatgttancaagatttg 668
           ||||||||||||||||||||||||
Sbjct: 645 agaaaaacatgttancaagatttg 668


 Score = 42.1 bits (21), Expect = 0.001
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 523 gaaaccgatcaagaaattgaa 543
           |||||||||||||||||||||
Sbjct: 523 gaaaccgatcaagaaattgaa 543


 Score = 36.2 bits (18), Expect = 0.091
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 598 aaccaacaatttataaat 615
           ||||||||||||||||||
Sbjct: 598 aaccaacaatttataaat 615


 Score = 30.2 bits (15), Expect = 5.6
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                          
Query: 728 aatagtattagtagt 742
           |||||||||||||||
Sbjct: 812 aatagtattagtagt 826


>Contig-U13938-1 (Contig-U13938-1Q) /CSM_Contig/Contig-U13938-1Q.Seq.d
          Length = 1111

 Score = 48.1 bits (24), Expect = 2e-05
 Identities = 24/24 (100%)
 Strand = Plus / Minus

                                    
Query: 1028 tcttcatcttcatcctcttcttca 1051
            ||||||||||||||||||||||||
Sbjct: 296  tcttcatcttcatcctcttcttca 273


>Contig-U12803-1 (Contig-U12803-1Q) /CSM_Contig/Contig-U12803-1Q.Seq.d
          Length = 2242

 Score = 46.1 bits (23), Expect = 9e-05
 Identities = 29/31 (93%)
 Strand = Plus / Minus

                                           
Query: 1020 cttcaacttcttcatcttcatcctcttcttc 1050
            ||||||| ||||| |||||||||||||||||
Sbjct: 634  cttcaacctcttcttcttcatcctcttcttc 604


 Score = 42.1 bits (21), Expect = 0.001
 Identities = 27/29 (93%)
 Strand = Plus / Minus

                                         
Query: 1022 tcaacttcttcatcttcatcctcttcttc 1050
            ||||||||||| |||||| ||||||||||
Sbjct: 647  tcaacttcttcttcttcaacctcttcttc 619


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 19,239
Number of Sequences: 6905
Number of extensions: 19239
Number of successful extensions: 2671
Number of sequences better than 10.0: 427
length of query: 1279
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1263
effective length of database: 5,564,391
effective search space: 7027825833
effective search space used: 7027825833
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.30
Homology vs DNA 
Query= Contig-U12467-1 (Contig-U12467-1Q) /CSM_Contig/Contig-U12467-1Q.Seq.d
         (1279 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440314) Dictyostelium discoideum cDNA clone:ddv43f08, 3' ...   815   0.0    3
(BJ421626) Dictyostelium discoideum cDNA clone:ddv43f08, 5' ...   523   0.0    3
(AC168612) Strongylocentrotus purpuratus clone R3-3046H17, W...    62   5e-05  1
(FE082212) LV_GL_RA21A16f Litopenaeus vannamei gills cDNA li...    48   2e-04  2
(AC013471) Homo sapiens BAC clone RP11-489A14 from 2, comple...    60   2e-04  1
(FE082211) LV_GL_RA21M14r Litopenaeus vannamei gills cDNA li...    48   3e-04  2
(AK226136) Homo sapiens mRNA for B/K protein variant, clone:...    56   0.003  1
(EX818830) CBNA6277.rev CBNA Phycomyces blakesleeanus NRRL15...    42   0.004  2
(EX819764) CBNA6732.rev CBNA Phycomyces blakesleeanus NRRL15...    42   0.004  2
(AL844509) Plasmodium falciparum chromosome 13.                    46   0.007  16
(EX570890) S0261 Pisum sativum shoot apical meristem ESTs Pi...    52   0.008  2
(EX570892) S0121 Pisum sativum shoot apical meristem ESTs Pi...    52   0.008  2
(AC176135) Strongylocentrotus purpuratus clone R3-3019P7, WO...    44   0.008  6
(AC177520) Strongylocentrotus purpuratus clone R3-9N24, WORK...    40   0.009  4
(AC137822) Medicago truncatula clone mth2-31e20, complete se...    54   0.013  1
(AC126009) Medicago truncatula clone mth2-15c20, complete se...    54   0.013  1
(AC073650) Homo sapiens 12 BAC RP11-44F24 (Roswell Park Canc...    54   0.013  1
(AC141319) Homo sapiens chromosome 16 clone XXfos-88388B11, ...    54   0.013  1
(AC137521) Medicago truncatula clone mth2-11o9, WORKING DRAF...    54   0.013  1
(AC202475) Medicago truncatula clone mth2-154n19, WORKING DR...    54   0.013  1
(AC009417) Homo sapiens clone RP11-44F24, WORKING DRAFT SEQU...    54   0.013  1
(DV121346) CV03028B2G02.f3 CV03-normalized library Euphorbia...    54   0.013  1
(DV120601) CV03026B2B09.f2 CV03-normalized library Euphorbia...    54   0.013  1
(CX782482) HESC3_14_E09.g1_A036 NIH_MGC_260 Homo sapiens cDN...    54   0.013  1
(EX570894) S0640 Pisum sativum shoot apical meristem ESTs Pi...    52   0.014  2
(EX570893) S1045 Pisum sativum shoot apical meristem ESTs Pi...    52   0.015  2
(AE014837) Plasmodium falciparum 3D7 chromosome 11 section 2...    40   0.016  11
(AC200974) Strongylocentrotus purpuratus clone R3-113B14, WO...    36   0.017  8
(AC184623) Strongylocentrotus purpuratus clone R3-3074I8, WO...    44   0.025  3
(AC149906) Strongylocentrotus purpuratus clone R3-15L2, WORK...    46   0.029  5
(AL844507) Plasmodium falciparum chromosome 8.                     46   0.033  18
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    42   0.034  8
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    44   0.035  12
(BX088567) Mouse DNA sequence from clone RP23-21L20 on chrom...    42   0.037  7
(AM444354) Vitis vinifera, whole genome shotgun sequence, co...    52   0.053  1
(AC105795) Rattus norvegicus clone CH230-254H13, *** SEQUENC...    52   0.053  1
(AC209773) Zea mays chromosome 7 clone CH201-118E14; ZMMBBc0...    52   0.053  1
(AC205155) Zea mays chromosome 4 clone CH201-163F20; ZMMBBc0...    52   0.053  1
(AC191057) Zea mays chromosome 3 clone CH201-7P8; ZMMBBc0007...    52   0.053  1
(AC191001) Zea mays chromosome 3 clone CH201-129N8; ZMMBBc01...    52   0.053  1
(AC184396) Strongylocentrotus purpuratus clone R3-3106K1, WO...    52   0.053  1
(AC184394) Strongylocentrotus purpuratus clone R3-3101M23, W...    52   0.053  1
(AC180250) Strongylocentrotus purpuratus clone R3-33P18, WOR...    52   0.053  1
(AC179716) Strongylocentrotus purpuratus clone R3-1021E12, W...    52   0.053  1
(AC179460) Strongylocentrotus purpuratus clone R3-63G3, WORK...    52   0.053  1
(AC178124) Strongylocentrotus purpuratus clone R3-3074P13, W...    52   0.053  1
(AL248152) Tetraodon nigroviridis genome survey sequence T7 ...    52   0.053  1
(AL196862) Tetraodon nigroviridis genome survey sequence T7 ...    52   0.053  1
(DH472204) Monosiga ovata DNA, fosmid clone: MOF-018N14, gen...    52   0.053  1
(EE635521) CHEL6288.b1_P11.ab1 CHE(LMS) serpentine sunflower...    52   0.053  1
(BB977989) Plasmodium berghei strain ANKA cDNA clone:OK00799...    52   0.053  1
(EY375658) CAXA15744.fwd CAXA Helobdella robusta Subtracted ...    52   0.053  1
(Z74796) S.cerevisiae chromosome XV reading frame ORF YOL054w.     40   0.064  3
(BZ165402) CH230-258J5.TJ CHORI-230 Segment 2 Rattus norvegi...    44   0.068  2
(DY892437) CeleSEQ10668 Cunninghamella elegans pBluescript (...    50   0.070  2
(DY887960) CeleSEQ4832 Cunninghamella elegans pBluescript (E...    50   0.071  2
(DY891223) CeleSEQ7906 Cunninghamella elegans pBluescript (E...    50   0.077  2
(AL929352) Plasmodium falciparum strain 3D7, chromosome 5, s...    40   0.078  9
(AC176209) Strongylocentrotus purpuratus clone R3-3063K17, W...    36   0.091  5
(AC181763) Strongylocentrotus purpuratus clone R3-1036A17, W...    36   0.096  7
(CD195265) MS1-0088T-L353-C06-U.B MS1-0088 Schistosoma manso...    42   0.098  2
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    40   0.10   10
(CD094801) ME1-0002P-M103-E02-U.G ME1-0002 Schistosoma manso...    42   0.11   2
(AC177687) Strongylocentrotus purpuratus clone R3-1013I2, WO...    40   0.12   6
(AC180802) Strongylocentrotus purpuratus clone R3-3071G22, W...    40   0.12   2
(AC201643) Strongylocentrotus purpuratus clone R3-54F19, WOR...    40   0.12   2
(CD179655) MS1-0018T-R291-G07-U.G MS1-0018 Schistosoma manso...    42   0.13   2
(CD179647) MS1-0018T-R291-F01-U.G MS1-0018 Schistosoma manso...    42   0.13   2
(CD179564) MS1-0018T-R188-E02-U.G MS1-0018 Schistosoma manso...    42   0.13   2
(AC177684) Strongylocentrotus purpuratus clone R3-1013P4, WO...    40   0.13   2
(CD111614) ME1-0020T-V236-H11-U.B ME1-0020 Schistosoma manso...    42   0.13   2
(AC196763) Citrus sinensis clone CSRBBa-044K07, WORKING DRAF...    48   0.14   5
(AC178957) Strongylocentrotus purpuratus clone R3-3121J22, W...    44   0.14   6
(CP000721) Clostridium beijerinckii NCIMB 8052, complete gen...    38   0.14   17
(AC184398) Strongylocentrotus purpuratus clone R3-3113E7, WO...    40   0.14   4
(AC176497) Strongylocentrotus purpuratus clone R3-3072G14, W...    40   0.14   6
(AC178192) Strongylocentrotus purpuratus clone R3-1116K10, W...    38   0.14   4
(CD111258) ME1-0020T-V183-A06-U.B ME1-0020 Schistosoma manso...    42   0.15   2
(AC094877) Rattus norvegicus clone CH230-5O3, WORKING DRAFT ...    50   0.15   3
(AL183832) Tetraodon nigroviridis genome survey sequence PUC...    34   0.16   2
(AC176408) Strongylocentrotus purpuratus clone R3-3044P19, W...    48   0.17   5
(CR293506) Zebrafish DNA sequence from clone CH211-144M15 in...    50   0.21   1
(BX649256) Zebrafish DNA sequence from clone DKEYP-92A5 in l...    50   0.21   1
(BX511023) Zebrafish DNA sequence from clone DKEY-148A12 in ...    50   0.21   1
(BC060940) Danio rerio zgc:73359, mRNA (cDNA clone MGC:73359...    50   0.21   1
(AL929378) Zebrafish DNA sequence from clone CH211-169B18.         50   0.21   1
(BC166424) Rattus norvegicus eukaryotic translation initiati...    50   0.21   1
(AC192065) Pan troglodytes BAC clone CH251-274G4 from chromo...    50   0.21   1
(AM434713) Vitis vinifera contig VV78X042222.13, whole genom...    50   0.21   1
(DJ470491) Method for analysing genome using microsatellite ...    50   0.21   1
(AC103580) Trypanosoma brucei chromosome 4 clone RPCI93-5E12...    50   0.21   1
(AC106013) Homo sapiens chromosome 8, clone RP11-1109L18, co...    50   0.21   1
(AC147276) Pan troglodytes chromosome UNK clone RP43-107K22,...    50   0.21   1
(AC140759) Rattus norvegicus clone CH230-48I18, *** SEQUENCI...    50   0.21   1
(AC124898) Rattus norvegicus clone CH230-4B17, *** SEQUENCIN...    50   0.21   1
(AC124884) Rattus norvegicus clone CH230-357C21, *** SEQUENC...    50   0.21   1
(AC106171) Rattus norvegicus chromosome 18 clone CH230-110O2...    50   0.21   1
(AC105607) Rattus norvegicus chromosome 18 clone CH230-102G1...    50   0.21   1
(AC103297) Rattus norvegicus clone CH230-21N14, WORKING DRAF...    50   0.21   1
(AC097048) Rattus norvegicus clone CH230-179E14, *** SEQUENC...    50   0.21   1

>(BJ440314) Dictyostelium discoideum cDNA clone:ddv43f08, 3' end,
            single read.
          Length = 636

 Score =  815 bits (411), Expect(3) = 0.0
 Identities = 420/420 (100%)
 Strand = Plus / Minus


Query: 853  tagttcctcaatatcatcttcaaatacattaaatagtggtgatagtaaaaatagtaaaga 912
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 428  tagttcctcaatatcatcttcaaatacattaaatagtggtgatagtaaaaatagtaaaga 369


Query: 913  tgaaaaacaatcttcttctaaagattcacagaaacaagaccatgataaaaaagaaaatga 972
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 368  tgaaaaacaatcttcttctaaagattcacagaaacaagaccatgataaaaaagaaaatga 309


Query: 973  ttcaagtttaattttgtcaattacaaaattaatgtgtggcccagtggcttcaacttcttc 1032
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 308  ttcaagtttaattttgtcaattacaaaattaatgtgtggcccagtggcttcaacttcttc 249


Query: 1033 atcttcatcctcttcttcaaacactgaatcaatatactttaaggttttaattgatggtgt 1092
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 248  atcttcatcctcttcttcaaacactgaatcaatatactttaaggttttaattgatggtgt 189


Query: 1093 agagtttaacaatgttaaattcttatcaatatctccacaatggggtactcatgttaaata 1152
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 188  agagtttaacaatgttaaattcttatcaatatctccacaatggggtactcatgttaaata 129


Query: 1153 tttccctgttcaatcatttgttgaacaaaaacaatctgacaaccaagaaaatcaccataa 1212
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 128  tttccctgttcaatcatttgttgaacaaaaacaatctgacaaccaagaaaatcaccataa 69


Query: 1213 taatcataatcataatcatcaccatcatacaaannatatttattcagnattaataataaa 1272
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 68   taatcataatcataatcatcaccatcatacaaannatatttattcagnattaataataaa 9

 Score =  176 bits (89), Expect(3) = 0.0
 Identities = 89/89 (100%)
 Strand = Plus / Minus


Query: 689 ggaaatagtagcgttttaagtggaatcagtaataatatcaatagtattagtagtggaatt 748
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 592 ggaaatagtagcgttttaagtggaatcagtaataatatcaatagtattagtagtggaatt 533


Query: 749 aatagtaacattggaaataataatcaaaa 777
           |||||||||||||||||||||||||||||
Sbjct: 532 aatagtaacattggaaataataatcaaaa 504

 Score = 42.1 bits (21), Expect(3) = 0.0
 Identities = 24/24 (100%)
 Strand = Plus / Minus


Query: 645 agaaaaacatgttancaagatttg 668
           ||||||||||||||||||||||||
Sbjct: 636 agaaaaacatgttancaagatttg 613

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,251,809,742
Number of extensions: 82733813
Number of successful extensions: 7271270
Number of sequences better than 10.0: 1114
Length of query: 1279
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1255
Effective length of database: 97,308,875,965
Effective search space: 122122639336075
Effective search space used: 122122639336075
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 6
Homology vs Protein 
Query= Contig-U12467-1 (Contig-U12467-1Q) /CSM_Contig/Contig-U12467-1Q.Seq.d
         (1279 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF531599_1(EF531599|pid:none) Danio rerio phosphofurin acidic cl...    52   6e-05
BC142600_1(BC142600|pid:none) Danio rerio phosphofurin acidic cl...    51   8e-05
EF531598_1(EF531598|pid:none) Danio rerio phosphofurin acidic cl...    51   8e-05
AY320283_1(AY320283|pid:none) Homo sapiens endosome-TGN sorting ...    47   0.001
(Q8K212) RecName: Full=Phosphofurin acidic cluster sorting prote...    47   0.001
BC009936_1(BC009936|pid:none) Homo sapiens phosphofurin acidic c...    47   0.001
BC003173_1(BC003173|pid:none) Homo sapiens phosphofurin acidic c...    47   0.001
(O88588) RecName: Full=Phosphofurin acidic cluster sorting prote...    47   0.001
(Q9ULP5) RecName: Full=Phosphofurin acidic cluster sorting prote...    47   0.001
AL137271_1(AL137271|pid:none) Homo sapiens mRNA; cDNA DKFZp434H1...    47   0.001
AK293966_1(AK293966|pid:none) Homo sapiens cDNA FLJ58767 complet...    47   0.001
BC055288_1(BC055288|pid:none) Homo sapiens phosphofurin acidic c...    47   0.001
AB033001_1(AB033001|pid:none) Homo sapiens mRNA for KIAA1175 pro...    47   0.001
BC131591_1(BC131591|pid:none) Homo sapiens phosphofurin acidic c...    47   0.002
AB385359_1(AB385359|pid:none) Synthetic construct DNA, clone: pF...    47   0.002
(Q86VP3) RecName: Full=Phosphofurin acidic cluster sorting prote...    47   0.002
BC050351_1(BC050351|pid:none) Homo sapiens phosphofurin acidic c...    47   0.002
AK030003_1(AK030003|pid:none) Mus musculus adult male testis cDN...    46   0.003
BC043302_1(BC043302|pid:none) Mus musculus phosphofurin acidic c...    46   0.003
AE014297_3578(AE014297|pid:none) Drosophila melanogaster chromos...    44   0.016
FN357542_10(FN357542|pid:none) Schistosoma mansoni genome sequen...    43   0.028
(Q82045) RecName: Full=Non-structural protein 1;          Short=...    35   4.5
EF672613_1(EF672613|pid:none) Human rotavirus A strain ST3 non-s...    35   4.5
Z32534_1(Z32534|pid:none) Human rotavirus (St Thomas) gene 5 (15...    35   4.5
FJ152122_1(FJ152122|pid:none) Human rotavirus A isolate US6597 i...    35   7.6
EF990709_1(EF990709|pid:none) Rotavirus A strain B3458 NSP1 gene...    35   7.6
FJ152133_1(FJ152133|pid:none) Human rotavirus A isolate US6668 i...    35   7.6
EU839966_1(EU839966|pid:none) Human rotavirus G12 strain SK423 n...    35   7.6
(Q82044) RecName: Full=Non-structural protein 1;          Short=...    34   9.9
EF672585_1(EF672585|pid:none) Human rotavirus A strain IAL28 non...    34   9.9
EU542705_1(EU542705|pid:none) Bovine rotavirus strain K55 NSP1 g...    34   9.9
(B3SRS0) RecName: Full=Non-structural protein 1;          Short=...    34   9.9

>EF531599_1(EF531599|pid:none) Danio rerio phosphofurin acidic cluster
            sorting protein 2 mRNA, complete cds.
          Length = 878

 Score = 51.6 bits (122), Expect = 6e-05
 Identities = 27/73 (36%), Positives = 40/73 (54%)
 Frame = +2

Query: 965  ENDSSLILSITKLMCGPVAXXXXXXXXXXNTESIYFKVLIDGVEFNNVKFLSISPQWGTH 1144
            E+ S +I  I++L+C                +    KVLIDGVE+N+VKF  ++ QW +H
Sbjct: 815  ESKSQVIEGISRLIC------------TAKHQQTMLKVLIDGVEWNDVKFFQLAAQWSSH 862

Query: 1145 VKYFPVQSFVEQK 1183
            VK+FP+  F   K
Sbjct: 863  VKHFPLAIFGPSK 875

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,004,898,266
Number of extensions: 16193046
Number of successful extensions: 31187
Number of sequences better than 10.0: 32
Number of HSP's gapped: 30930
Number of HSP's successfully gapped: 32
Length of query: 426
Length of database: 1,051,180,864
Length adjustment: 131
Effective length of query: 295
Effective length of database: 627,191,635
Effective search space: 185021532325
Effective search space used: 185021532325
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.81  gvh: 0.42  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U12467-1 is cyt
VS (DIR, S) 0
VH (FL, L) 1
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0