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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U12350-1
Contig ID Contig-U12350-1
Contig update 2002.12.18
Contig sequence 
>Contig-U12350-1 (Contig-U12350-1Q) /CSM_Contig/Contig-U12350-1Q.Seq.d
CACTGTTGGCCTACTGGNATAATAATACACATTTTACAAATAGTAGAGGA
GGTCATAGAGGTGGATCATCAAGAGGTGGATCATCAAGAGGTGGTAACAG
TGGATCATCAAGAGGTGGATCAAGAGGTGGATATAGAGGTCAATCATCAT
TTTATAATTCATCATACCAAAAGTCAGGTCCAAGATTATTGACTAGCATC
GTATCACTTCCACCATTTAGTGGTGGTGCAATCATTGGTGTCAAAGGTTC
ATTATGGGGTTTATTGAATAAAGCTACCACCGCAAGATTAAAGGTATTCA
AGAATACAGCAATCATTAAGTGTGCCGACGCACAACAATTGGATAGAGCC
AAAGAGATCGTTCTAAAGTTGAAGAATACCTCACCAAAATACTTGTCGCT
CATCGATTACAAAGGTGAACAAAAGATTAAGTTTGTTCATCAACCATCAT
TTTCAGAGGTGATTTTAGAGAGTGGTGCTATCGATCCATTGGCCACTGAT
GGCGTTCAATTGATGCAAGATTTGATCAACTCCCATGAATCTCAATATGT
CGCACGTTTAAATGAAACCTTTTCAACTCTCTACAGNGAAAAAGGNTCTT
CCACCCCTTTCATANACATTGAANCCGNNNAAATATGGNNNNNNNNN---
-------CCACCACCAACACTCAACAATCACCAAATAGCGACTCATCAGA
ATATAAATTCACTGATCCATTCATTTATAAACTTAATCACATTAATAGTT
CAAAAGTACTTTTCCCTCAAATTAAATCTAGTTCTGATCTTCGTGTTACA
ATCGACACCCACTCCAAGAGTAAATTGGTTAGCGGAACTGGTCAACATCA
ATACGACAATCTCATCAAATTTATCGATTCCATTAAAATTCATAAAAACT
CCTCTGGTAATAACACCTATCAAATTCCAGATAGCAATCTTTTCATGACT
GAATCAATTATCACTCGTAAGGTCTTAATGTATAGATCCGATGATAATAG
CCTTCAATTCCATGTTAATGAGGACATTGCAACTCGTTACACAACTTCAA
CCCCCGAAGTTAACAGAGAATCCACTACCGTATTCTGTACTTCTCCTGCC
CTTTACGATCTCTTCAAATCAAAGAATTGGACCGTCGATCAAGCTTGCTG
ATGAATTAAAGAAACTCTCTAAAAATCTTAGACTTTTGGTTTCAAAACAT
TTAGATTCAACTAAATTGCCAACCACCTACAGCTGATCATACTACCCAAT
TCGAACAAGATGATGAAGAAGAAGAGAATTAAAAAAAAAAAAAAAAAAAA
AAAAAAAAATTNAAAAAAAA
Gap gap included
Contig length 1310
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 1570208
End point 1568925
Strand (PLUS/MINUS) MINUS
Number of clones 5
Number of EST 6
Link to clone list U12350
List of clone(s) 

est1=VFL306F,1,638
est2=AFJ446Z,639,1259
est3=VFL306Z,639,1259
est4=VHE863Z,639,1126
est5=VFH115Z,775,1229
est6=VSG384F,1117,1302
Translated Amino Acid sequence 
LLAYWXNNTHFTNSRGGHRGGSSRGGSSRGGNSGSSRGGSRGGYRGQSSFYNSSYQKSGP
RLLTSIVSLPPFSGGAIIGVKGSLWGLLNKATTARLKVFKNTAIIKCADAQQLDRAKEIV
LKLKNTSPKYLSLIDYKGEQKIKFVHQPSFSEVILESGAIDPLATDGVQLMQDLINSHES
QYVARLNETFSTLYXEKGSSTPFIXIEXXXIWXXX---

---TTNTQQSPNSDSSEYKFTDPFIYKLNHINSSKVLFPQIKSSSDLRVTIDTHSKSKLV
SGTGQHQYDNLIKFIDSIKIHKNSSGNNTYQIPDSNLFMTESIITRKVLMYRSDDNSLQF
HVNEDIATRYTTSTPEVNRESTTVFCTSPALYDLFKSKNWTVDQAC**iketl*ks*tfg
fktfrfn*ianhlqliilpnsnkmmkkkrikkkkkkkkknxkk
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgiiihilqiveevievdhqevdhqevvtvdhqevdqevdievnhhfiihhtksqv
qdy*lasyhfhhlvvvqslvskvhygvy*iklppqd*rysriqqslsvpthnnwiepkrs
f*s*riphqntcrssitkvnkrlslfinhhfqr*f*rvvlsihwplmafn*cki*stpmn
lnmshv*mkpfqlstxkkxlpplsxtlxpxkygxx---

---ppptlnnhqiathqninslihsfinlitlivqkyfslklnlvlifvlqstptprvnw
laelvninttissnlsiplkfiktplvitpikfqiaifs*lnqlslvrs*cidpmiiafn
smlmrtlqlvtqlqppkltenplpysvlllpftissnqrigpsikladelkklsknlrll
vskhldstklpttys*syypirtr**rrrelkkkkkkkkkixkk

Frame B:
tvgllx**ytfyk**rrs*rwiikrwiikrw*qwiikrwikrwi*rsiiil*fiipkvrs
kiid*hritsti*wwcnhwcqrfimgfie*syhrkikgiqeysnh*vcrrttig*sqrdr
skveeyltkilvahrlqr*tkd*vcsstiifrgdfrewcyrsigh*wrsidarfdqlp*i
sicrtfk*nlfnslqxkrxfhpfhxh*xrxnmxxx---

---hhqhstitk*rliri*ih*sihl*t*sh**fkstfpsn*i*f*sscynrhplqe*ig
*rnwstsirqshqiyrfh*ns*kllw**hlsnsr*qsfhd*inyhs*glnv*ir***psi
pc**ghcnslhnfnprs*qrihyrilyfscplrslqikeldrrssllmn*rnslkildfw
fqni*iqlncqpptadhttqfeqddeeeen*kkkkkkkkkxkk

Frame C:
LLAYWXNNTHFTNSRGGHRGGSSRGGSSRGGNSGSSRGGSRGGYRGQSSFYNSSYQKSGP
RLLTSIVSLPPFSGGAIIGVKGSLWGLLNKATTARLKVFKNTAIIKCADAQQLDRAKEIV
LKLKNTSPKYLSLIDYKGEQKIKFVHQPSFSEVILESGAIDPLATDGVQLMQDLINSHES
QYVARLNETFSTLYXEKGSSTPFIXIEXXXIWXXX---

---TTNTQQSPNSDSSEYKFTDPFIYKLNHINSSKVLFPQIKSSSDLRVTIDTHSKSKLV
SGTGQHQYDNLIKFIDSIKIHKNSSGNNTYQIPDSNLFMTESIITRKVLMYRSDDNSLQF
HVNEDIATRYTTSTPEVNRESTTVFCTSPALYDLFKSKNWTVDQAC**iketl*ks*tfg
fktfrfn*ianhlqliilpnsnkmmkkkrikkkkkkkkknxkk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U12350-1 (Contig-U12350-1Q)
/CSM_Contig/Contig-U12350-1Q.Seq.d
         (1320 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U12350-1 (Contig-U12350-1Q) /CSM_Contig/Conti...  1235  0.0
Contig-U09318-1 (Contig-U09318-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U13288-1 (Contig-U13288-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U14571-1 (Contig-U14571-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U11931-1 (Contig-U11931-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U12626-1 (Contig-U12626-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U12286-1 (Contig-U12286-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U11930-1 (Contig-U11930-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U11475-1 (Contig-U11475-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U11262-1 (Contig-U11262-1Q) /CSM_Contig/Conti...    38  0.024

>Contig-U12350-1 (Contig-U12350-1Q) /CSM_Contig/Contig-U12350-1Q.Seq.d
          Length = 1320

 Score = 1235 bits (623), Expect = 0.0
 Identities = 623/623 (100%)
 Strand = Plus / Plus

                                                                        
Query: 658  ccaccaccaacactcaacaatcaccaaatagcgactcatcagaatataaattcactgatc 717
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 658  ccaccaccaacactcaacaatcaccaaatagcgactcatcagaatataaattcactgatc 717

                                                                        
Query: 718  cattcatttataaacttaatcacattaatagttcaaaagtacttttccctcaaattaaat 777
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 718  cattcatttataaacttaatcacattaatagttcaaaagtacttttccctcaaattaaat 777

                                                                        
Query: 778  ctagttctgatcttcgtgttacaatcgacacccactccaagagtaaattggttagcggaa 837
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 778  ctagttctgatcttcgtgttacaatcgacacccactccaagagtaaattggttagcggaa 837

                                                                        
Query: 838  ctggtcaacatcaatacgacaatctcatcaaatttatcgattccattaaaattcataaaa 897
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 838  ctggtcaacatcaatacgacaatctcatcaaatttatcgattccattaaaattcataaaa 897

                                                                        
Query: 898  actcctctggtaataacacctatcaaattccagatagcaatcttttcatgactgaatcaa 957
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 898  actcctctggtaataacacctatcaaattccagatagcaatcttttcatgactgaatcaa 957

                                                                        
Query: 958  ttatcactcgtaaggtcttaatgtatagatccgatgataatagccttcaattccatgtta 1017
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 958  ttatcactcgtaaggtcttaatgtatagatccgatgataatagccttcaattccatgtta 1017

                                                                        
Query: 1018 atgaggacattgcaactcgttacacaacttcaacccccgaagttaacagagaatccacta 1077
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1018 atgaggacattgcaactcgttacacaacttcaacccccgaagttaacagagaatccacta 1077

                                                                        
Query: 1078 ccgtattctgtacttctcctgccctttacgatctcttcaaatcaaagaattggaccgtcg 1137
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1078 ccgtattctgtacttctcctgccctttacgatctcttcaaatcaaagaattggaccgtcg 1137

                                                                        
Query: 1138 atcaagcttgctgatgaattaaagaaactctctaaaaatcttagacttttggtttcaaaa 1197
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1138 atcaagcttgctgatgaattaaagaaactctctaaaaatcttagacttttggtttcaaaa 1197

                                                                        
Query: 1198 catttagattcaactaaattgccaaccacctacagctgatcatactacccaattcgaaca 1257
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1198 catttagattcaactaaattgccaaccacctacagctgatcatactacccaattcgaaca 1257

                                   
Query: 1258 agatgatgaagaagaagagaatt 1280
            |||||||||||||||||||||||
Sbjct: 1258 agatgatgaagaagaagagaatt 1280


 Score = 1217 bits (614), Expect = 0.0
 Identities = 638/638 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   cactgttggcctactggnataataatacacattttacaaatagtagaggaggtcatagag 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   cactgttggcctactggnataataatacacattttacaaatagtagaggaggtcatagag 60

                                                                       
Query: 61  gtggatcatcaagaggtggatcatcaagaggtggtaacagtggatcatcaagaggtggat 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  gtggatcatcaagaggtggatcatcaagaggtggtaacagtggatcatcaagaggtggat 120

                                                                       
Query: 121 caagaggtggatatagaggtcaatcatcattttataattcatcataccaaaagtcaggtc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 caagaggtggatatagaggtcaatcatcattttataattcatcataccaaaagtcaggtc 180

                                                                       
Query: 181 caagattattgactagcatcgtatcacttccaccatttagtggtggtgcaatcattggtg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 caagattattgactagcatcgtatcacttccaccatttagtggtggtgcaatcattggtg 240

                                                                       
Query: 241 tcaaaggttcattatggggtttattgaataaagctaccaccgcaagattaaaggtattca 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tcaaaggttcattatggggtttattgaataaagctaccaccgcaagattaaaggtattca 300

                                                                       
Query: 301 agaatacagcaatcattaagtgtgccgacgcacaacaattggatagagccaaagagatcg 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 agaatacagcaatcattaagtgtgccgacgcacaacaattggatagagccaaagagatcg 360

                                                                       
Query: 361 ttctaaagttgaagaatacctcaccaaaatacttgtcgctcatcgattacaaaggtgaac 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 ttctaaagttgaagaatacctcaccaaaatacttgtcgctcatcgattacaaaggtgaac 420

                                                                       
Query: 421 aaaagattaagtttgttcatcaaccatcattttcagaggtgattttagagagtggtgcta 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 aaaagattaagtttgttcatcaaccatcattttcagaggtgattttagagagtggtgcta 480

                                                                       
Query: 481 tcgatccattggccactgatggcgttcaattgatgcaagatttgatcaactcccatgaat 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 tcgatccattggccactgatggcgttcaattgatgcaagatttgatcaactcccatgaat 540

                                                                       
Query: 541 ctcaatatgtcgcacgtttaaatgaaaccttttcaactctctacagngaaaaaggntctt 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ctcaatatgtcgcacgtttaaatgaaaccttttcaactctctacagngaaaaaggntctt 600

                                                 
Query: 601 ccacccctttcatanacattgaanccgnnnaaatatgg 638
           ||||||||||||||||||||||||||||||||||||||
Sbjct: 601 ccacccctttcatanacattgaanccgnnnaaatatgg 638


>Contig-U09318-1 (Contig-U09318-1Q) /CSM_Contig/Contig-U09318-1Q.Seq.d
          Length = 1951

 Score = 44.1 bits (22), Expect = 4e-04
 Identities = 25/25 (100%)
 Strand = Plus / Plus

                                   
Query: 2  actgttggcctactggnataataat 26
          |||||||||||||||||||||||||
Sbjct: 1  actgttggcctactggnataataat 25


>Contig-U13288-1 (Contig-U13288-1Q) /CSM_Contig/Contig-U13288-1Q.Seq.d
          Length = 172

 Score = 42.1 bits (21), Expect = 0.002
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 2  actgttggcctactggnataataa 25
          ||||||||||||||||||||||||
Sbjct: 1  actgttggcctactggnataataa 24


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 12,176
Number of Sequences: 6905
Number of extensions: 12176
Number of successful extensions: 1660
Number of sequences better than 10.0: 553
length of query: 1320
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1304
effective length of database: 5,564,391
effective search space: 7255965864
effective search space used: 7255965864
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.30
Homology vs DNA 
Query= Contig-U12350-1 (Contig-U12350-1Q) /CSM_Contig/Contig-U12350-1Q.Seq.d
         (1320 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415617) Dictyostelium discoideum cDNA clone:ddv23l01, 5' ...  1213   0.0    1
(BJ433855) Dictyostelium discoideum cDNA clone:ddv23l01, 3' ...   969   0.0    3
(BJ344467) Dictyostelium discoideum cDNA clone:dda19l12, 3' ...   954   0.0    3
(BJ439510) Dictyostelium discoideum cDNA clone:ddv40n16, 3' ...   454   0.0    2
(BJ431700) Dictyostelium discoideum cDNA clone:ddv15m03, 3' ...   747   0.0    2
(AU266399) Dictyostelium discoideum vegetative cDNA clone:VS...   139   8e-57  2
(EC762168) PSE00000178 rw_mgpallid Polysphondylium pallidum ...    60   3e-11  2
(BJ423409) Dictyostelium discoideum cDNA clone:ddv49i03, 5' ...    52   0.055  1
(AM754179) Clytia hemisphaerica EST, 5' end sequence, clone ...    42   0.056  2
(AM756095) Clytia hemisphaerica EST, 5' end sequence, clone ...    42   0.063  2
(AM755215) Clytia hemisphaerica EST, 5' end sequence, clone ...    42   0.063  2
(CU432254) Clytia hemisphaerica 5-PRIME EST from clone SA0AA...    42   0.065  2
(CU431874) Clytia hemisphaerica 5-PRIME EST from clone SA0AA...    42   0.072  2
(CU433552) Clytia hemisphaerica 5-PRIME EST from clone SA0AA...    42   0.074  2
(CU428747) Clytia hemisphaerica 5-PRIME EST from clone SA0AA...    42   0.080  2
(AC115592) Dictyostelium discoideum chromosome 2 map 1-12595...    42   0.13   2
(BJ336903) Dictyostelium discoideum cDNA clone:dda55g13, 5' ...    36   0.69   2
(S60885) LYAR=cell growth regulating nucleolar protein [mice...    48   0.85   1
(AC115931) Mus musculus chromosome 5, clone RP24-535K22, com...    48   0.85   1
(AC084071) Mus musculus strain C57BL/6J chromosome 5 clone r...    48   0.85   1
(AK006373) Mus musculus adult male testis cDNA, RIKEN full-l...    48   0.85   1
(EK355181) 1095468500447 Global-Ocean-Sampling_GS-31-01-01-1...    48   0.85   1
(EG564866) 01010001086.F11_0104230987 MacDonald peritubular ...    48   0.85   1
(EG564695) 01050007086.F11_00102713XE MacDonald pachytene sp...    48   0.85   1
(AV516971) Mus musculus cDNA, Abe mouse ES cell cDNA library...    48   0.85   1
(AV459448) Mus musculus cDNA, Abe mouse ES cell cDNA library...    48   0.85   1
(DT903057) PCRS40532 Hematopoietic stem cells Mus musculus c...    48   0.85   1
(AV166232) Mus musculus 13-day embryo head cDNA, partial seq...    48   0.85   1
(AV083336) Mus musculus adult male tongue cDNA, partial sequ...    48   0.85   1
(AU044713) Mus musculus 16-cell embryo cDNA 3'-end sequence,...    48   0.85   1
(CR514589) RZPD Mus musculus cDNA clone LIONp462A094 3' EST.       48   0.85   1
(CJ319512) Mus musculus 14.5 days embryo Rathke's pouches cD...    48   0.85   1
(CJ303667) Mus musculus 14.5 days embryo Rathke's pouches cD...    48   0.85   1
(CJ299140) Mus musculus 14.5 days embryo Rathke's pouches cD...    48   0.85   1
(CJ232163) Mus musculus adult male testis cDNA, RIKEN full-l...    48   0.85   1
(CJ217708) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ216044) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ215263) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ214673) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ214474) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ214408) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ214116) Mus musculus 5 days embryo whole body cDNA, RIKEN...    48   0.85   1
(CJ208013) Mus musculus morula whole body morula cDNA, RIKEN...    48   0.85   1
(CF583192) AGENCOURT_8797622_updated NIH_MGC_137 Mus musculu...    48   0.85   1
(CF384027) lac01d10.y1 SiEP Mus musculus cDNA 5' similar to ...    48   0.85   1
(CF259705) lab36a12.y1 Normalized colon epithelial progenito...    48   0.85   1
(CD776102) UI-M-AQ0-cja-p-24-0-UI.s1 NIH_BMAP_MHI Mus muscul...    48   0.85   1
(CD776101) UI-M-AQ0-cja-p-22-0-UI.s1 NIH_BMAP_MHI Mus muscul...    48   0.85   1
(CD740932) UI-M-AO0-chy-k-18-0-UI.s1 NIH_BMAP_MPG Mus muscul...    48   0.85   1
(CB274508) mai70a04.y1 McCarrey Eddy spermatocytes Mus muscu...    48   0.85   1
(CA466276) AGENCOURT_10727681 NIH_MGC_169 Mus musculus cDNA ...    48   0.85   1
(BY659402) Mus musculus visual cortex cDNA, RIKEN full-lengt...    48   0.85   1
(BY647314) Mus musculus visual cortex cDNA, RIKEN full-lengt...    48   0.85   1
(AI786498) uf14h11.y1 Soares_mammary_gland_NMLMG Mus musculu...    48   0.85   1
(AI507567) vj79f10.x1 Knowles Solter mouse blastocyst B1 Mus...    48   0.85   1
(BY614813) Mus musculus visual cortex cDNA, RIKEN full-lengt...    48   0.85   1
(BY451363) Mus musculus adult male testis cDNA, RIKEN full-l...    48   0.85   1
(BY393033) Mus musculus 12 days pregnant adult female placen...    48   0.85   1
(BY387550) Mus musculus blastocyst blastocyst cDNA, RIKEN fu...    48   0.85   1
(BU944428) AGENCOURT_10545194 NIH_MGC_107 Homo sapiens cDNA ...    48   0.85   1
(BU936205) AGENCOURT_10522023 NIH_MGC_169 Mus musculus cDNA ...    48   0.85   1
(BU935830) AGENCOURT_10521171 NIH_MGC_169 Mus musculus cDNA ...    48   0.85   1
(BQ031867) UI-1-CF0-aov-a-10-0-UI.s1 NCI_CGAP_PlTr1 Mus musc...    48   0.85   1
(AI195971) ue52d11.r1 Soares_mammary_gland_NMLMG Mus musculu...    48   0.85   1
(BM206512) C0298F05-3 NIA Mouse 7.5-dpc Whole Embryo cDNA Li...    48   0.85   1
(BM206145) C0293H05-3 NIA Mouse 7.5-dpc Whole Embryo cDNA Li...    48   0.85   1
(BM202676) C0243A09-3 NIA Mouse 7.5-dpc Whole Embryo cDNA Li...    48   0.85   1
(BM198020) C0353C09-3 NIA Mouse Undifferentiated ES Cell cDN...    48   0.85   1
(BJ396913) Dictyostelium discoideum cDNA clone:dds44l10, 5' ...    48   0.85   1
(BJ370342) Dictyostelium discoideum cDNA clone:ddc53e19, 5' ...    48   0.85   1
(BJ336987) Dictyostelium discoideum cDNA clone:dda55i23, 5' ...    48   0.85   1
(BI790348) id06d11.x1 Melton Normalized Mixed Mouse Pancreas...    48   0.85   1
(BG071774) H3103A10-3 NIA Mouse 15K cDNA Clone Set Mus muscu...    48   0.85   1
(AA795511) vq96a04.r1 Knowles Solter mouse blastocyst B3 Mus...    48   0.85   1
(BF318830) uy06b04.x1 McCarrey Eddy spermatocytes Mus muscul...    48   0.85   1
(BF019007) uy01a11.x1 McCarrey Eddy spermatocytes Mus muscul...    48   0.85   1
(BF017326) uw74d06.x1 Soares_mammary_gland_NMLMG Mus musculu...    48   0.85   1
(BE650286) UI-M-BH2.1-app-f-07-0-UI.r1 NIH_BMAP_M_S3.1 Mus m...    48   0.85   1
(BB837690) Mus musculus mammary gland RCB-0527 Jyg-MC(B) cDN...    48   0.85   1
(BB831982) Mus musculus mammary gland RCB-0527 Jyg-MC(B) cDN...    48   0.85   1
(AW987147) uf14h11.x1 Soares_mammary_gland_NMLMG Mus musculu...    48   0.85   1
(AW320973) uo15a06.x1 NCI_CGAP_Mam6 Mus musculus cDNA clone ...    48   0.85   1
(AW123891) UI-M-BH2.1-app-f-07-0-UI.s1 NIH_BMAP_M_S3.1 Mus m...    48   0.85   1
(AW121110) UI-M-BH2.3-aoh-b-11-0-UI.s1 NIH_BMAP_M_S3.3 Mus m...    48   0.85   1
(CU425533) Clytia hemisphaerica 5-PRIME EST from clone SA0AA...    42   0.99   2
(CJ467026) Macaca fascicularis mRNA, clone: QnpA-16457, 5' e...    34   2.1    3
(AC203348) Zea mays chromosome 10 clone ZMMBBb-276E6; ZMMBBb...    40   2.1    2
(FE254479) CAPH5076.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(FE250617) CAPH2957.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(FE252313) CAPH3946.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(FE256807) CAPH6299.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(FE231819) CAPG1421.fwd CAPG Naegleria gruberi amoeba stage ...    36   2.3    2
(FE248911) CAPH1974.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(FE253024) CAPH4315.fwd CAPH Naegleria gruberi amoeba stage ...    36   2.3    2
(AZ178986) SP_0163_A1_B01_SP6E Strongylocentrotus purpuratus...    40   2.4    2
(BJ415626) Dictyostelium discoideum cDNA clone:ddv23n01, 5' ...    36   3.3    2
(CU368751) Mouse DNA sequence from clone DN-239K2 on chromos...    46   3.4    1
(AC101299) Mus musculus chromosome 1, clone RP23-103M17, com...    46   3.4    1
(BT019204) Zea mays clone Contig877.F mRNA sequence.               46   3.4    1
(AC006081) Arabidopsis thaliana chromosome 2 clone T2G17 map...    46   3.4    1

>(BJ415617) Dictyostelium discoideum cDNA clone:ddv23l01, 5' end,
           single read.
          Length = 638

 Score = 1213 bits (612), Expect = 0.0
 Identities = 627/627 (100%)
 Strand = Plus / Plus


Query: 1   cactgttggcctactggnataataatacacattttacaaatagtagaggaggtcatagag 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   cactgttggcctactggnataataatacacattttacaaatagtagaggaggtcatagag 60


Query: 61  gtggatcatcaagaggtggatcatcaagaggtggtaacagtggatcatcaagaggtggat 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  gtggatcatcaagaggtggatcatcaagaggtggtaacagtggatcatcaagaggtggat 120


Query: 121 caagaggtggatatagaggtcaatcatcattttataattcatcataccaaaagtcaggtc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 caagaggtggatatagaggtcaatcatcattttataattcatcataccaaaagtcaggtc 180


Query: 181 caagattattgactagcatcgtatcacttccaccatttagtggtggtgcaatcattggtg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 caagattattgactagcatcgtatcacttccaccatttagtggtggtgcaatcattggtg 240


Query: 241 tcaaaggttcattatggggtttattgaataaagctaccaccgcaagattaaaggtattca 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tcaaaggttcattatggggtttattgaataaagctaccaccgcaagattaaaggtattca 300


Query: 301 agaatacagcaatcattaagtgtgccgacgcacaacaattggatagagccaaagagatcg 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 agaatacagcaatcattaagtgtgccgacgcacaacaattggatagagccaaagagatcg 360


Query: 361 ttctaaagttgaagaatacctcaccaaaatacttgtcgctcatcgattacaaaggtgaac 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 ttctaaagttgaagaatacctcaccaaaatacttgtcgctcatcgattacaaaggtgaac 420


Query: 421 aaaagattaagtttgttcatcaaccatcattttcagaggtgattttagagagtggtgcta 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 aaaagattaagtttgttcatcaaccatcattttcagaggtgattttagagagtggtgcta 480


Query: 481 tcgatccattggccactgatggcgttcaattgatgcaagatttgatcaactcccatgaat 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 tcgatccattggccactgatggcgttcaattgatgcaagatttgatcaactcccatgaat 540


Query: 541 ctcaatatgtcgcacgtttaaatgaaaccttttcaactctctacagngaaaaaggntctt 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ctcaatatgtcgcacgtttaaatgaaaccttttcaactctctacagngaaaaaggntctt 600


Query: 601 ccacccctttcatanacattgaanccg 627
           |||||||||||||||||||||||||||
Sbjct: 601 ccacccctttcatanacattgaanccg 627

 Score = 46.1 bits (23), Expect(3) = 2e-09
 Identities = 23/23 (100%)
 Strand = Plus / Plus


Query: 72 agaggtggatcatcaagaggtgg 94
          |||||||||||||||||||||||
Sbjct: 57 agaggtggatcatcaagaggtgg 79

 Score = 46.1 bits (23), Expect(3) = 2e-09
 Identities = 23/23 (100%)
 Strand = Plus / Plus


Query: 57 agaggtggatcatcaagaggtgg 79
          |||||||||||||||||||||||
Sbjct: 72 agaggtggatcatcaagaggtgg 94

 Score = 38.2 bits (19), Expect(3) = 2e-09
 Identities = 19/19 (100%)
 Strand = Plus / Plus


Query: 100 gtggatcatcaagaggtgg 118
           |||||||||||||||||||
Sbjct: 76  gtggatcatcaagaggtgg 94

 Score = 38.2 bits (19), Expect(3) = 2e-09
 Identities = 19/19 (100%)
 Strand = Plus / Plus


Query: 76  gtggatcatcaagaggtgg 94
           |||||||||||||||||||
Sbjct: 100 gtggatcatcaagaggtgg 118

 Score = 28.2 bits (14), Expect(3) = 2e-09
 Identities = 14/14 (100%)
 Strand = Plus / Plus


Query: 119 atcaagaggtggat 132
           ||||||||||||||
Sbjct: 107 atcaagaggtggat 120

 Score = 28.2 bits (14), Expect(3) = 2e-09
 Identities = 14/14 (100%)
 Strand = Plus / Plus


Query: 107 atcaagaggtggat 120
           ||||||||||||||
Sbjct: 119 atcaagaggtggat 132

 Score = 46.1 bits (23), Expect = 3.4
 Identities = 23/23 (100%)
 Strand = Plus / Plus


Query: 100 gtggatcatcaagaggtggatca 122
           |||||||||||||||||||||||
Sbjct: 61  gtggatcatcaagaggtggatca 83

 Score = 46.1 bits (23), Expect = 3.4
 Identities = 23/23 (100%)
 Strand = Plus / Plus


Query: 61  gtggatcatcaagaggtggatca 83
           |||||||||||||||||||||||
Sbjct: 100 gtggatcatcaagaggtggatca 122

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,266,489,937
Number of extensions: 72808054
Number of successful extensions: 5449776
Number of sequences better than 10.0: 141
Length of query: 1320
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1296
Effective length of database: 97,308,875,965
Effective search space: 126112303250640
Effective search space used: 126112303250640
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 2
Homology vs Protein 
Query= Contig-U12350-1 (Contig-U12350-1Q) /CSM_Contig/Contig-U12350-1Q.Seq.d
         (1320 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Q48) RecName: Full=Putative uncharacterized protein DDB_G028...   327   7e-95
(Q5M323) RecName: Full=UPF0348 protein stu1615;  &CP000023_1488(...    37   1.2
CP000847_51(CP000847|pid:none) Rickettsia akari str. Hartford, c...    37   1.6
CR940347_561(CR940347|pid:none) Theileria annulata strain Ankara...    36   2.7
BC172286_1(BC172286|pid:none) Synthetic construct Homo sapiens c...    36   3.5
(Q8NAP3) RecName: Full=Zinc finger and BTB domain-containing pro...    36   3.5
EU401641_1(EU401641|pid:none) Nothobranchius melanospilus vouche...    35   4.6
FM992691_94(FM992691|pid:none) Candida dubliniensis CD36 chromos...    35   6.0
AE014188_377(AE014188|pid:none) Plasmodium falciparum 3D7 chromo...    35   6.0
AB308463_2(AB308463|pid:none) Aeluroscalabotes felinus mitochond...    35   6.0
AL844501_57(AL844501|pid:none) Plasmodium falciparum strain 3D7,...    35   7.9
EU401639_1(EU401639|pid:none) Nothobranchius melanospilus vouche...    35   7.9

>(Q54Q48) RecName: Full=Putative uncharacterized protein DDB_G0284097;
          Length = 633

 Score =  327 bits (837), Expect(2) = 7e-95
 Identities = 161/162 (99%), Positives = 161/162 (99%)
 Frame = +3

Query: 660  TTNTQQSPNSDSSEYKFTDPFIYKLNHINSSKVLFPQIKSSSDLRVTIDTHSKSKLVSGT 839
            TTNTQQSPNSDSSEYKFTDPFIYKLNHINSSKVLFPQIKS SDLRVTIDTHSKSKLVSGT
Sbjct: 428  TTNTQQSPNSDSSEYKFTDPFIYKLNHINSSKVLFPQIKSGSDLRVTIDTHSKSKLVSGT 487

Query: 840  GQHQYDNLIKFIDSIKIHKNSSGNNTYQIPDSNLFMTESIITRKVLMYRSDDNSLQFHVN 1019
            GQHQYDNLIKFIDSIKIHKNSSGNNTYQIPDSNLFMTESIITRKVLMYRSDDNSLQFHVN
Sbjct: 488  GQHQYDNLIKFIDSIKIHKNSSGNNTYQIPDSNLFMTESIITRKVLMYRSDDNSLQFHVN 547

Query: 1020 EDIATRYTTSTPEVNRESTTVFCTSPALYDLFKSKNWTVDQA 1145
            EDIATRYTTSTPEVNRESTTVFCTSPALYDLFKSKNWTVDQA
Sbjct: 548  EDIATRYTTSTPEVNRESTTVFCTSPALYDLFKSKNWTVDQA 589

 Score = 45.8 bits (107), Expect(2) = 7e-95
 Identities = 22/23 (95%), Positives = 23/23 (100%)
 Frame = +1

Query: 1147 ADELKKLSKNLRLLVSKHLDSTK 1215
            +DELKKLSKNLRLLVSKHLDSTK
Sbjct: 590  SDELKKLSKNLRLLVSKHLDSTK 612

 Score =  314 bits (804), Expect = 5e-84
 Identities = 186/328 (56%), Positives = 212/328 (64%), Gaps = 9/328 (2%)
 Frame = +3

Query: 21   NNTHFTNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFYNSSYQKSGPRLLTSI 200
            NNTHFTN                                    FYNSSYQKSGPRLLTSI
Sbjct: 140  NNTHFTNSRGGHRGGSSRGGSSRGGNSGSSRGGSRGGYRGQSSFYNSSYQKSGPRLLTSI 199

Query: 201  VSLPPFSGGAIIGVKGSLWGLLNKATTARLKVFKNTAIIKCADAQQLDRAKEIVLKLKNT 380
            VSLPPFSGGAIIGVKGSLWGLLNKATTARLKVFKNTAIIKCADAQQLDRAKEIVLKLKNT
Sbjct: 200  VSLPPFSGGAIIGVKGSLWGLLNKATTARLKVFKNTAIIKCADAQQLDRAKEIVLKLKNT 259

Query: 381  SPKYLSLIDYKGEQKIKFVHQPSFSEVILESGAIDPLATDGVQLMQDLINSHESQYVARL 560
            SPKYLSLIDYKGEQKIKFVHQPSFSEVILESGAIDPLATDGVQLMQDLINSHESQYVARL
Sbjct: 260  SPKYLSLIDYKGEQKIKFVHQPSFSEVILESGAIDPLATDGVQLMQDLINSHESQYVARL 319

Query: 561  NETFSTLYXEKGSSTPFIXIEXXXIWXXXXXXXTTNTQQSPNSDSSEYKFTDPF--IYKL 734
            NETFSTLY EKGSSTPFI IE   IW       ++N   +P +      +T+    +++
Sbjct: 320  NETFSTLYSEKGSSTPFIDIEAGKIW----YLHSSNIPSTPMTADKYELYTESLASVFQP 375

Query: 735  NHI-------NSSKVLFPQIKSSSDLRVTIDTHSKSKLVSGTGQHQYDNLIKFIDSIKIH 893
            +++       +S   LF Q K  + + V +DT S   L     + +         + +
Sbjct: 376  SNVINEQFITSSQYKLFKQKKQFTFISV-LDTESLDLLCIKCSEEKITPTTTTTTNTQQS 434

Query: 894  KNSSGNNTYQIPDSNLFMTESIITRKVL 977
             NS  ++ Y+  D  ++    I + KVL
Sbjct: 435  PNSD-SSEYKFTDPFIYKLNHINSSKVL 461

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,964,364,706
Number of extensions: 38749684
Number of successful extensions: 97874
Number of sequences better than 10.0: 12
Number of HSP's gapped: 97811
Number of HSP's successfully gapped: 14
Length of query: 440
Length of database: 1,051,180,864
Length adjustment: 132
Effective length of query: 308
Effective length of database: 623,955,076
Effective search space: 192178163408
Effective search space used: 192178163408
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.74  gvh: 0.38  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.52  mip: 0.16
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: mitochondrial
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for Contig-U12350-1 is mit
VS (DIR, S) 1
VH (FL, L) 1
VF (FL, S) 2
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0