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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U12257-1
Contig ID Contig-U12257-1
Contig update 2002.12.18
Contig sequence 
>Contig-U12257-1 (Contig-U12257-1Q) /CSM_Contig/Contig-U12257-1Q.Seq.d
ATAAATAAATAAAATTTAATATATATATATATATATATATATAAATATAT
ACATACATATATATATAAAATGACAGATAAAATTAATGGAGAGATTGAAA
TACCATATAGTTTATTTAAATCAATAGAATTAGGATCAGAGAAAAATATT
CCATTG----------TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTAGTACCATATTTTATTTAATTTTTATTTTTTTTTTTATTTTT
TTTTTATAAAACATAATATATTATAAAATAATTTTGACCTTAATAATAAT
AAAAATAAATTTAGAAAATGATTGAGAATTTATCTTCTAGCTTTTTGTTT
TAAAGATTTATAATCAAAACCAAATGATAAATCTTTATCACCAGATAATA
AAATTTGTTCAAAACAATAAGAATGAATTGTTTTTGGAATATCAACGAAA
ACTGGAACAACACCTATTCTTTTATTACCACCACCACCACCTCTACCACC
ATAAGCACTACTTTTTTTAACGCTCATAAATGATGAAGCTTTGGCTTTCT
CTTGATATTCAGCTAAATCACAAACACTATCATTTCTATTTCCAAATCCA
CGTGAACGAATACCAGGTGCACCATATATATTAATATTATTATTATTATT
ATTTGAAGTTGGTTGAGAAGCTGAAGGTGGAGTCGAAGAAAAGTAAGAAA
CTTGTTTAAGAACACCATTAAAATTTGAAGCCACAGTACCTTGTGGTAAA
TAAACTGTAGCGAAACAAGCTCTTAATGGCAAATCAATCTTTGGAATTAT
GAAATTGAATTTACCTTTACGTGTTTTCTCATTGAATTTATGGAGTTCCT
CTTGTAAAATAACGATTTCAATCTTGATTGGAGTATTTTGGTCGAAAACA
CCGGGTTTTAAAATTGGGATTAATAAAGCACCATCGTTCTCAATGATTGA
AGGTTTACAAGGATCACCATCCATCATACAAGACCAAACCTCGTATCCGA
ATCCAATTTGAACCTTTAGGAATTGTTTTTGAGTATTTTTAATATTTAAA
ATCATTTGGTGGATCATTTTTCCACTATAACTCACTGTTGTTACCCAACT
CATTTCCTCAACGATCGATACGAGTACAGCACAATCTAAATTTCTTTTAA
CTCTTAATTTTAGCTGGAAATGTGGTTCCAAGAATTTATAACTTAGGAGA
ATTGGATGATTTGCCATTTTACATAATTCAATTGGTAATTCTCTAACATC
AACTACACTTAGTACATCATTACCAATATCTGAAATCTCAACATTGGTAC
GTGCTTCAACACCAAGAAAACCACGTTGTCTTGAAATTTCATCACCTTTA
CAAATCATTGATGGTATATTCAATTCACCACTTGTATCCTTCATTGAATA
TTCACCATTAATGTCAATACTATAATTTGTATCAAATCCATAATCTAATT
GAATTTGTATCACTCTTTTATGTTTTGGTGATTCAATTCTCTCCAATGTC
AACAACTTCCCATCTTTTAATTGCTTTACCATTAACACTTATAATATTAA
CATTATCTTCAATTTCAATATTAAATGATGATAATGTACCTGCAATAATT
TTATAATCAATTTTATATTTTAATAATAATAAACCTTCACCAATTGAACA
AAGTATATTTTGTGAAACTATAACATTTGGTTTAACTACAACTTTTGGTT
GTGC
Gap gap included
Contig length 1694
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 8
Number of EST 15
Link to clone list U12257
List of clone(s) 

est1=SSJ613F,1,157
est2=SFB167F,158,751
est3=VSJ759F,238,706
est4=VSJ759Z,238,689
est5=CHD351F,246,844
est6=CFH665F,299,947
est7=CFG533F,366,904
est8=SLB218Z,402,857
est9=SSJ613Z,500,885
est10=CFG533Z,910,1565
est11=CFH665Z,945,1559
est12=SFB167Z,1000,1582
est13=SLB218F,1098,1696
est14=CHD351Z,1311,1439
est15=CHA782Z,1312,1439
Translated Amino Acid sequence 
ink*NLIYIYIYIYKYIHTYIYKMTDKINGEIEIPYSLFKSIELGSEKNIPL---

---FFFFFFFFFFFFFFSTIFYLIFIFFFIFFL*niiyykiiltliiikinlend*efif
*lfvlkiynqnqminlyhqiikfvqnnknelfleyqrkleqhlffyyhhhhlyhhkhyff
*rs*mmklwlsldiqlnhkhyhfyfqihvneyqvhhiy*yyyyyylklveklkveskksk
klv*ehh*nlkpqylvvnkl*rnkllmanqslel*n*iylyvfsh*iygvplvk*rfqs*
leyfgrkhrvlklglikhhrsq*lkvykdhhpsyktkpririqfeplgivfeyf*ylksf
ggsffhynslllpnsfpqrsirvqhnlnff*llilagnvvpriynlgelddlpfyiiqlv
il*hqlhlvhhyqylksqhwyvlqhqenhvvlkfhhlykslmvysihhlypslnihh*cq
yynlyqihnliefvslfyvlviqfspmsttshllialpltliiltlssisilnddnvpai
il*silyfnnnkpspieqsifcetitfgltttfgc
Translated Amino Acid sequence (All Frames) 
Frame A:
ink*NLIYIYIYIYKYIHTYIYKMTDKINGEIEIPYSLFKSIELGSEKNIPL---

---FFFFFFFFFFFFFFSTIFYLIFIFFFIFFL*niiyykiiltliiikinlend*efif
*lfvlkiynqnqminlyhqiikfvqnnknelfleyqrkleqhlffyyhhhhlyhhkhyff
*rs*mmklwlsldiqlnhkhyhfyfqihvneyqvhhiy*yyyyyylklveklkveskksk
klv*ehh*nlkpqylvvnkl*rnkllmanqslel*n*iylyvfsh*iygvplvk*rfqs*
leyfgrkhrvlklglikhhrsq*lkvykdhhpsyktkpririqfeplgivfeyf*ylksf
ggsffhynslllpnsfpqrsirvqhnlnff*llilagnvvpriynlgelddlpfyiiqlv
il*hqlhlvhhyqylksqhwyvlqhqenhvvlkfhhlykslmvysihhlypslnihh*cq
yynlyqihnliefvslfyvlviqfspmsttshllialpltliiltlssisilnddnvpai
il*silyfnnnkpspieqsifcetitfgltttfgc

Frame B:
*inki*yiyiyiyiniyihiyik*qiklmerlkyhivylnq*n*dqrkifh---

---ffffffffffffflvpyfi*flffflfffykt*yiik*f*p****k*i*kmienlss
sflf*rfiiktk**ifitr**nlfktirmncfwninenwnntysfitttttsttisttff
nahk**sfgfllifs*itntiisiskst*tntrctiyiniiiiii*swlrs*rwsrrkvr
nlfkntiki*shstlw*incsetss*wqinlwnyeieftftcfliefmefll*nndfnld
wsilventgf*nwd**stivlnd*rftritihhtrpnlvsesnlnl*elflsifni*nhl
vdhfstithccypthflndryeysti*isfns*f*lemwfqefit*enwmichft*fnw*
fsninyt*yiitni*nlnigtcfntkkttls*nfitftnh*wyiqfttcilh*iftinvn
tiiciksii*lnlyhsfmfw*fnslqcqqlpif*llyh*hl*y*hylqfqy*mmimylq*
fynqfyiliiinlhqlnkvyfvkl*hlv*lqllvv

Frame C:
k*ikfniyiyiyi*iytyiyi*ndr*n*wrd*nti*fi*inririrekysi---

---fffffffffffff*yhilfnfyfffyfffikhnil*nnfdlnnnknkfrk*lriyll
afcfkdl*skpndkslspdnkicskq*e*ivfgistktgttpilllppppplpp*allfl
tlindealafs*ysaksqtlsflfpnpreripgapyililllllfevg*eaeggveek*e
tclrtplkfeatvpcgk*tvakqalngksifgimklnlplrvfslnlwsssckitisili
gvfwsktpgfkiginkapsfsmieglqgspsiiqdqtsypnpi*tfrncf*vflifkiiw
wiifpl*ltvvtqlisstidtstaqskflltlnfswkcgsknl*lrrig*failhnsign
sltsttlstslpiseistlvrastprkprcleissplqiidgifnsplvsfieysplmsi
l*fvsnp*sn*icitllcfgdsilsnvnnfpsfncftintyniniifnfnik***ctcnn
fiinfif****tftn*tkyil*nyniwfnynfwlc
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U12257-1 (Contig-U12257-1Q)
/CSM_Contig/Contig-U12257-1Q.Seq.d
         (1704 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U12257-1 (Contig-U12257-1Q) /CSM_Contig/Conti...  2083  0.0
Contig-U14366-1 (Contig-U14366-1Q) /CSM_Contig/Conti...   100  1e-20
Contig-U11698-1 (Contig-U11698-1Q) /CSM_Contig/Conti...    46  1e-04
Contig-U10801-1 (Contig-U10801-1Q) /CSM_Contig/Conti...    46  1e-04
Contig-U10318-1 (Contig-U10318-1Q) /CSM_Contig/Conti...    46  1e-04
Contig-U14187-1 (Contig-U14187-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U11803-1 (Contig-U11803-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U11283-1 (Contig-U11283-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U11181-1 (Contig-U11181-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U10863-1 (Contig-U10863-1Q) /CSM_Contig/Conti...    42  0.002

>Contig-U12257-1 (Contig-U12257-1Q) /CSM_Contig/Contig-U12257-1Q.Seq.d
          Length = 1704

 Score = 2083 bits (1051), Expect = 0.0
 Identities = 1051/1051 (100%)
 Strand = Plus / Plus

                                                                        
Query: 654  tgaagttggttgagaagctgaaggtggagtcgaagaaaagtaagaaacttgtttaagaac 713
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 654  tgaagttggttgagaagctgaaggtggagtcgaagaaaagtaagaaacttgtttaagaac 713

                                                                        
Query: 714  accattaaaatttgaagccacagtaccttgtggtaaataaactgtagcgaaacaagctct 773
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 714  accattaaaatttgaagccacagtaccttgtggtaaataaactgtagcgaaacaagctct 773

                                                                        
Query: 774  taatggcaaatcaatctttggaattatgaaattgaatttacctttacgtgttttctcatt 833
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 774  taatggcaaatcaatctttggaattatgaaattgaatttacctttacgtgttttctcatt 833

                                                                        
Query: 834  gaatttatggagttcctcttgtaaaataacgatttcaatcttgattggagtattttggtc 893
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 834  gaatttatggagttcctcttgtaaaataacgatttcaatcttgattggagtattttggtc 893

                                                                        
Query: 894  gaaaacaccgggttttaaaattgggattaataaagcaccatcgttctcaatgattgaagg 953
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 894  gaaaacaccgggttttaaaattgggattaataaagcaccatcgttctcaatgattgaagg 953

                                                                        
Query: 954  tttacaaggatcaccatccatcatacaagaccaaacctcgtatccgaatccaatttgaac 1013
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 954  tttacaaggatcaccatccatcatacaagaccaaacctcgtatccgaatccaatttgaac 1013

                                                                        
Query: 1014 ctttaggaattgtttttgagtatttttaatatttaaaatcatttggtggatcatttttcc 1073
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1014 ctttaggaattgtttttgagtatttttaatatttaaaatcatttggtggatcatttttcc 1073

                                                                        
Query: 1074 actataactcactgttgttacccaactcatttcctcaacgatcgatacgagtacagcaca 1133
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1074 actataactcactgttgttacccaactcatttcctcaacgatcgatacgagtacagcaca 1133

                                                                        
Query: 1134 atctaaatttcttttaactcttaattttagctggaaatgtggttccaagaatttataact 1193
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1134 atctaaatttcttttaactcttaattttagctggaaatgtggttccaagaatttataact 1193

                                                                        
Query: 1194 taggagaattggatgatttgccattttacataattcaattggtaattctctaacatcaac 1253
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1194 taggagaattggatgatttgccattttacataattcaattggtaattctctaacatcaac 1253

                                                                        
Query: 1254 tacacttagtacatcattaccaatatctgaaatctcaacattggtacgtgcttcaacacc 1313
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1254 tacacttagtacatcattaccaatatctgaaatctcaacattggtacgtgcttcaacacc 1313

                                                                        
Query: 1314 aagaaaaccacgttgtcttgaaatttcatcacctttacaaatcattgatggtatattcaa 1373
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1314 aagaaaaccacgttgtcttgaaatttcatcacctttacaaatcattgatggtatattcaa 1373

                                                                        
Query: 1374 ttcaccacttgtatccttcattgaatattcaccattaatgtcaatactataatttgtatc 1433
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1374 ttcaccacttgtatccttcattgaatattcaccattaatgtcaatactataatttgtatc 1433

                                                                        
Query: 1434 aaatccataatctaattgaatttgtatcactcttttatgttttggtgattcaattctctc 1493
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1434 aaatccataatctaattgaatttgtatcactcttttatgttttggtgattcaattctctc 1493

                                                                        
Query: 1494 caatgtcaacaacttcccatcttttaattgctttaccattaacacttataatattaacat 1553
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1494 caatgtcaacaacttcccatcttttaattgctttaccattaacacttataatattaacat 1553

                                                                        
Query: 1554 tatcttcaatttcaatattaaatgatgataatgtacctgcaataattttataatcaattt 1613
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1554 tatcttcaatttcaatattaaatgatgataatgtacctgcaataattttataatcaattt 1613

                                                                        
Query: 1614 tatattttaataataataaaccttcaccaattgaacaaagtatattttgtgaaactataa 1673
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1614 tatattttaataataataaaccttcaccaattgaacaaagtatattttgtgaaactataa 1673

                                           
Query: 1674 catttggtttaactacaacttttggttgtgc 1704
            |||||||||||||||||||||||||||||||
Sbjct: 1674 catttggtttaactacaacttttggttgtgc 1704


 Score =  579 bits (292), Expect = e-165
 Identities = 306/313 (97%)
 Strand = Plus / Plus

                                                                       
Query: 311 ttagaaaatgattgagaatttatcttctagctttttgttttaaagatttataatcaaaac 370
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 311 ttagaaaatgattgagaatttatcttctagctttttgttttaaagatttataatcaaaac 370

                                                                       
Query: 371 caaatgataaatctttatcaccagataataaaatttgttcaaaacaataagaatgaattg 430
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 371 caaatgataaatctttatcaccagataataaaatttgttcaaaacaataagaatgaattg 430

                                                                       
Query: 431 tttttggaatatcaacgaaaactggaacaacacctattcttttattaccaccaccaccac 490
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 431 tttttggaatatcaacgaaaactggaacaacacctattcttttattaccaccaccaccac 490

                                                                       
Query: 491 ctctaccaccataagcactacnnnnnnnaacgctcataaatgatgaagctttggctttct 550
           |||||||||||||||||||||       ||||||||||||||||||||||||||||||||
Sbjct: 491 ctctaccaccataagcactactttttttaacgctcataaatgatgaagctttggctttct 550

                                                                       
Query: 551 cttgatattcagctaaatcacaaacactatcatttctatttccaaatccacgtgaacgaa 610
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 551 cttgatattcagctaaatcacaaacactatcatttctatttccaaatccacgtgaacgaa 610

                        
Query: 611 taccaggtgcacc 623
           |||||||||||||
Sbjct: 611 taccaggtgcacc 623


 Score =  176 bits (89), Expect = 5e-44
 Identities = 89/89 (100%)
 Strand = Plus / Plus

                                                                       
Query: 68  aaatgacagataaaattaatggagagattgaaataccatatagtttatttaaatcaatag 127
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 68  aaatgacagataaaattaatggagagattgaaataccatatagtttatttaaatcaatag 127

                                        
Query: 128 aattaggatcagagaaaaatattccattg 156
           |||||||||||||||||||||||||||||
Sbjct: 128 aattaggatcagagaaaaatattccattg 156


 Score = 36.2 bits (18), Expect = 0.12
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                            
Query: 1  ataaataaataaaattta 18
          ||||||||||||||||||
Sbjct: 1  ataaataaataaaattta 18


>Contig-U14366-1 (Contig-U14366-1Q) /CSM_Contig/Contig-U14366-1Q.Seq.d
          Length = 931

 Score = 99.6 bits (50), Expect = 1e-20
 Identities = 86/98 (87%)
 Strand = Plus / Minus

                                                                        
Query: 1006 atttgaacctttaggaattgtttttgagtatttttaatatttaaaatcatttggtggatc 1065
            ||||||| |||||||||||||||||| ||||||||||||  ||| | |||||| || |||
Sbjct: 169  atttgaatctttaggaattgtttttgtgtatttttaataagtaataccatttgatgaatc 110

                                                  
Query: 1066 atttttccactataactcactgttgttacccaactcat 1103
            ||||| ||||||||||| || ||||| |||||||||||
Sbjct: 109  attttaccactataactaaccgttgtaacccaactcat 72


 Score = 67.9 bits (34), Expect = 3e-11
 Identities = 49/54 (90%)
 Strand = Plus / Minus

                                                                 
Query: 356 atttataatcaaaaccaaatgataaatctttatcaccagataataaaatttgtt 409
           |||||||||| |||||||| | ||||||||| ||||||||||||| ||||||||
Sbjct: 724 atttataatcgaaaccaaaagttaaatctttgtcaccagataatagaatttgtt 671


 Score = 52.0 bits (26), Expect = 2e-06
 Identities = 44/50 (88%)
 Strand = Plus / Minus

                                                             
Query: 877 attggagtattttggtcgaaaacaccgggttttaaaattgggattaataa 926
           |||||||||||||| || |||  ||| |||||||||||||| ||||||||
Sbjct: 298 attggagtattttgatcaaaacaaccaggttttaaaattggtattaataa 249


 Score = 34.2 bits (17), Expect = 0.48
 Identities = 17/17 (100%)
 Strand = Plus / Minus

                            
Query: 743 gtggtaaataaactgta 759
           |||||||||||||||||
Sbjct: 429 gtggtaaataaactgta 413


>Contig-U11698-1 (Contig-U11698-1Q) /CSM_Contig/Contig-U11698-1Q.Seq.d
          Length = 1510

 Score = 46.1 bits (23), Expect = 1e-04
 Identities = 26/27 (96%)
 Strand = Plus / Minus

                                       
Query: 477  accaccaccaccacctctaccaccata 503
            ||||||||||||||| |||||||||||
Sbjct: 1288 accaccaccaccaccactaccaccata 1262


 Score = 32.2 bits (16), Expect = 1.9
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                            
Query: 476  taccaccaccaccacc 491
            ||||||||||||||||
Sbjct: 1292 taccaccaccaccacc 1277


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 43,795
Number of Sequences: 6905
Number of extensions: 43795
Number of successful extensions: 6950
Number of sequences better than 10.0: 719
length of query: 1704
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1688
effective length of database: 5,564,391
effective search space: 9392692008
effective search space used: 9392692008
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.29
Homology vs DNA 
Query= Contig-U12257-1 (Contig-U12257-1Q) /CSM_Contig/Contig-U12257-1Q.Seq.d
         (1704 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ372805) Dictyostelium discoideum cDNA clone:ddc14a09, 3' ...  1150   0.0    2
(BJ374839) Dictyostelium discoideum cDNA clone:ddc16a18, 3' ...  1080   0.0    2
(BJ399000) Dictyostelium discoideum cDNA clone:dds3e17, 3' e...  1021   0.0    2
(BJ361268) Dictyostelium discoideum cDNA clone:ddc16a18, 5' ...   599   0.0    3
(AU060717) Dictyostelium discoideum slug cDNA, clone SLB218.      777   0.0    3
(BJ387587) Dictyostelium discoideum cDNA clone:dds3e17, 5' e...   470   0.0    3
(BJ359389) Dictyostelium discoideum cDNA clone:ddc14a09, 5' ...   458   0.0    4
(BJ363794) Dictyostelium discoideum cDNA clone:ddc28f13, 5' ...   313   0.0    4
(AU270429) Dictyostelium discoideum vegetative cDNA clone:VS...   311   e-163  3
(AU270428) Dictyostelium discoideum vegetative cDNA clone:VS...   287   e-161  4
(AU033601) Dictyostelium discoideum slug cDNA, clone SLB218.      311   e-134  2
(C90680) Dictyostelium discoideum slug cDNA, clone SSJ613.        295   9e-86  2
(BJ376444) Dictyostelium discoideum cDNA clone:ddc28f13, 3' ...   196   2e-45  1
(AU074139) Dictyostelium discoideum slug cDNA, clone SSJ613.      176   6e-43  2
(BJ377317) Dictyostelium discoideum cDNA clone:ddc23d21, 3' ...   159   3e-41  2
(AU266375) Dictyostelium discoideum vegetative cDNA clone:VS...   100   2e-27  3
(BJ381417) Dictyostelium discoideum cDNA clone:ddc41o12, 3' ...    68   3e-09  2
(AP009268) Solanum lycopersicum genomic DNA, chromosome 8, c...    42   0.012  2
(AX458634) Sequence 180 from Patent WO0246454.                     52   0.071  1
(AX346461) Sequence 1532 from Patent WO0200928.                    52   0.071  1
(AC116924) Dictyostelium discoideum chromosome 2 map 6357117...    36   0.15   10
(CL599223) OB__Ba0064J02.r OB__Ba Oryza brachyantha genomic ...    48   0.36   2
(AL929399) Mouse DNA sequence from clone RP23-59E24 on chrom...    42   0.84   4
(CU137665) Medicago truncatula chromosome 5 clone mth2-152c1...    36   1.0    7
(AM443459) Vitis vinifera contig VV78X278801.3, whole genome...    48   1.1    1
(CW870796) she3h2-63.b_045.ab1 Whole-genome shotgun library ...    48   1.1    1
(ER709934) SB_BBc105-J14.F SB_BBc Sorghum bicolor genomic 5'...    42   1.8    2
(AC103124) Rattus norvegicus clone CH230-226J15, WORKING DRA...    38   1.8    6
(AC149428) Populus trichocarpa clone Pop1-080J15, complete s...    46   1.8    5
(CU571336) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    44   2.5    5
(EI199907) ZMMBBc0490I24.fv ZMMBB_VAL Zea mays genomic clone...    36   3.1    2
(EA700736) Sequence 79615 from patent US 7365185.                  38   4.2    2
(CR854881) Zebrafish DNA sequence from clone CH211-198O8 in ...    46   4.4    1
(CR759744) Zebrafish DNA sequence from clone CH211-200N5 in ...    46   4.4    1
(BX465198) Zebrafish DNA sequence from clone DKEYP-84E12 in ...    46   4.4    1
(AC149479) Populus trichocarpa clone Pop1-017N13, complete s...    46   4.4    1
(AX458543) Sequence 89 from Patent WO0246454.                      46   4.4    1
(AC022884) Homo sapiens chromosome 18, clone RP11-449D8, com...    46   4.4    1
(CU463232) Zebrafish DNA sequence *** SEQUENCING CANCELLED *...    46   4.4    1
(AC225523) Medicago truncatula clone mth2-144h15, WORKING DR...    46   4.4    1
(AC222867) Bos taurus clone CH240-436D24, WORKING DRAFT SEQU...    46   4.4    1
(AC221906) Bos taurus clone CH240-378O24, WORKING DRAFT SEQU...    46   4.4    1
(EK398410) 1095469529085 Global-Ocean-Sampling_GS-31-01-01-1...    46   4.4    1
(EK294119) 1095462341037 Global-Ocean-Sampling_GS-31-01-01-1...    46   4.4    1
(EJ475598) 1095403441243 Global-Ocean-Sampling_GS-28-01-01-1...    46   4.4    1
(ED192528) AUAC-aac02g12.b1 Ascaris suum whole genome shotgu...    46   4.4    1
(CW126090) 104_506_11112703_116_34722_022 Sorghum methylatio...    46   4.4    1
(CL702407) SP__Ba0084E24.f SP__Ba Sorghum propinquum genomic...    46   4.4    1
(CG952788) MBEML09TF mth2 Medicago truncatula genomic clone ...    46   4.4    1
(BJ413265) Dictyostelium discoideum cDNA clone:ddv14k20, 5' ...    46   4.4    1
(BJ324779) Dictyostelium discoideum cDNA clone:dda7p21, 5' e...    46   4.4    1
(FC816280) Sr_pAMT7_05k22_T7 S. ratti mixed stage pAMP Stron...    46   4.4    1
(FC810832) Sr_pASP6_05k22_SP6 S. ratti mixed stage pAMP Stro...    46   4.4    1
(AE017261) Picrophilus torridus DSM 9790, complete genome.         46   4.4    1
(AP010034) Lotus japonicus genomic DNA, clone: LjT47I02, TM0...    36   4.5    4
(AM440258) Vitis vinifera contig VV78X164601.9, whole genome...    32   4.8    4
(CL601819) OB__Ba0071L21.r OB__Ba Oryza brachyantha genomic ...    44   5.1    2
(AC151571) Latimeria menadoensis clone VMRC4-140H19, complet...    42   5.3    6
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    32   5.7    13
(EK183691) 1095458134416 Global-Ocean-Sampling_GS-31-01-01-1...    42   5.7    2
(AC005536) Homo sapiens BAC clone GS1-115E6 from 7, complete...    40   5.9    4
(AC221639) Bos taurus clone CH240-389K19, WORKING DRAFT SEQU...    36   6.0    6
(DU053517) 141481 Tomato HindIII BAC Library Solanum lycoper...    36   6.1    2
(AC022186) Homo sapiens chromosome 11 clone RP11-157E8 map 1...    40   6.1    3
(ER389913) 1094428800132 Global-Ocean-Sampling_GS-34-01-01-1...    38   6.4    2
(EJ305118) 1095390072887 Global-Ocean-Sampling_GS-27-01-01-1...    36   6.4    2
(AR690418) Sequence 24 from patent US 6906240.                     38   6.8    2
(AR633163) Sequence 24 from patent US 6846677.                     38   6.8    2
(AR630864) Sequence 24 from patent US 6841721.                     38   6.8    2
(AR568227) Sequence 24 from patent US 6781036.                     38   6.8    2
(AR566957) Sequence 24 from patent US 6768042.                     38   6.8    2
(EA027506) Sequence 1 from patent US 7135621.                      38   6.8    2
(AX320925) Sequence 1 from Patent WO0179517.                       38   6.8    2
(AR794682) Sequence 1 from patent US 6998517.                      38   6.8    2
(AC127239) Mus musculus chromosome 8 clone RP24-381A23, WORK...    40   6.8    9
(AM054752) Isotricha prostoma EST, clone Ip05_A05.                 34   8.5    3
(AM054691) Isotricha prostoma EST, clone Ip03_D03.                 34   8.5    3
(AP000162) Homo sapiens genomic DNA, chromosome 21q22.2, DSC...    40   9.2    2
(AC172293) Bos taurus clone CH240-177O10, WORKING DRAFT SEQU...    40   9.4    3
(AM447292) Vitis vinifera contig VV78X208004.3, whole genome...    34   9.5    2
(AC124741) Mus musculus BAC clone RP23-357D17 from chromosom...    42   9.8    6

>(BJ372805) Dictyostelium discoideum cDNA clone:ddc14a09, 3' end,
            single read.
          Length = 656

 Score = 1150 bits (580), Expect(2) = 0.0
 Identities = 580/580 (100%)
 Strand = Plus / Minus


Query: 918  gattaataaagcaccatcgttctcaatgattgaaggtttacaaggatcaccatccatcat 977
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 656  gattaataaagcaccatcgttctcaatgattgaaggtttacaaggatcaccatccatcat 597


Query: 978  acaagaccaaacctcgtatccgaatccaatttgaacctttaggaattgtttttgagtatt 1037
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 596  acaagaccaaacctcgtatccgaatccaatttgaacctttaggaattgtttttgagtatt 537


Query: 1038 tttaatatttaaaatcatttggtggatcatttttccactataactcactgttgttaccca 1097
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 536  tttaatatttaaaatcatttggtggatcatttttccactataactcactgttgttaccca 477


Query: 1098 actcatttcctcaacgatcgatacgagtacagcacaatctaaatttcttttaactcttaa 1157
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 476  actcatttcctcaacgatcgatacgagtacagcacaatctaaatttcttttaactcttaa 417


Query: 1158 ttttagctggaaatgtggttccaagaatttataacttaggagaattggatgatttgccat 1217
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 416  ttttagctggaaatgtggttccaagaatttataacttaggagaattggatgatttgccat 357


Query: 1218 tttacataattcaattggtaattctctaacatcaactacacttagtacatcattaccaat 1277
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 356  tttacataattcaattggtaattctctaacatcaactacacttagtacatcattaccaat 297


Query: 1278 atctgaaatctcaacattggtacgtgcttcaacaccaagaaaaccacgttgtcttgaaat 1337
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 296  atctgaaatctcaacattggtacgtgcttcaacaccaagaaaaccacgttgtcttgaaat 237


Query: 1338 ttcatcacctttacaaatcattgatggtatattcaattcaccacttgtatccttcattga 1397
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 236  ttcatcacctttacaaatcattgatggtatattcaattcaccacttgtatccttcattga 177


Query: 1398 atattcaccattaatgtcaatactataatttgtatcaaatccataatctaattgaatttg 1457
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 176  atattcaccattaatgtcaatactataatttgtatcaaatccataatctaattgaatttg 117


Query: 1458 tatcactcttttatgttttggtgattcaattctctccaat 1497
            ||||||||||||||||||||||||||||||||||||||||
Sbjct: 116  tatcactcttttatgttttggtgattcaattctctccaat 77

 Score =  135 bits (68), Expect(2) = 0.0
 Identities = 68/68 (100%)
 Strand = Plus / Minus


Query: 1499 tcaacaacttcccatcttttaattgctttaccattaacacttataatattaacattatct 1558
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 76   tcaacaacttcccatcttttaattgctttaccattaacacttataatattaacattatct 17


Query: 1559 tcaatttc 1566
            ||||||||
Sbjct: 16   tcaatttc 9

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,761,142,754
Number of extensions: 112535972
Number of successful extensions: 11798892
Number of sequences better than 10.0: 83
Length of query: 1704
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1680
Effective length of database: 97,308,875,965
Effective search space: 163478911621200
Effective search space used: 163478911621200
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 2
Homology vs Protein 
Query= Contig-U12257-1 (Contig-U12257-1Q) /CSM_Contig/Contig-U12257-1Q.Seq.d
         (1704 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP007157_835(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...    35   8.4
AY689436_161(AY689436|pid:none) Deerpox virus W-848-83, complete...    35   8.4

>AP007157_835(AP007157|pid:none) Aspergillus oryzae RIB40 genomic DNA,
            SC023.
          Length = 368

 Score = 35.0 bits (79), Expect = 8.4
 Identities = 25/93 (26%), Positives = 43/93 (46%), Gaps = 4/93 (4%)
 Frame = -2

Query: 1496 LERIESPKHKRVIQIQLDYGFDTNYSIDINGEYSMKDTSGELNIPSMICKGDEISRQRGF 1317
            L  I SP  + V Q Q  Y  D + S+  N E+   D  G+LN+P       +  ++
Sbjct: 19   LNAIPSPYDQAVQQQQGSYNLDADLSLFTNAEFFDFDNFGDLNLPGFDSVESDRMKKENN 78

Query: 1316 LGVEARTNVEISDI-GNDVLSVV---DVRELPI 1230
                   ++E  D+ G+  +S+V   ++ ELP+
Sbjct: 79   QATGQNPDMEFLDLFGDYTVSIVFGPELEELPV 111

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 2,119,103,527
Number of extensions: 38124576
Number of successful extensions: 89176
Number of sequences better than 10.0: 2
Number of HSP's gapped: 89110
Number of HSP's successfully gapped: 2
Length of query: 568
Length of database: 1,051,180,864
Length adjustment: 134
Effective length of query: 434
Effective length of database: 617,481,958
Effective search space: 267987169772
Effective search space used: 267987169772
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.69  gvh: 0.32  alm: 0.18  top: 0.53  tms: 0.13  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 28.0 %: plasma membrane
	 24.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U12257-1 is end
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 1
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 1
CH (FL, L) 2
CF (FL, S) 2
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0