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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U12069-1
Contig ID Contig-U12069-1
Contig update 2002.12.18
Contig sequence 
>Contig-U12069-1 (Contig-U12069-1Q) /CSM_Contig/Contig-U12069-1Q.Seq.d
AAATTATTAATATATATATTAAAATGAAAATAAATAAAATTATTTTATTT
TTTTTCTTATCATGTTTATATTTATTTTCCTCTAGTGTATCAGCACAACA
ATTATTGGGAGAAATCGAAATTACTGAAAAAAGTTTTACAATCGATGCAT
CTGTTATTAATACAAAAATTAATTATTTAATAGATAATACAATAACAAAA
CCAAAATTATCAGTTACATCAATATTTGGTGAAGCGACAAGTATTGATGA
TATTCTTCGAATAAGTGATGATAAAAAGTCTTTAACATTTGGTGACTATA
CAAGTAGTACACTTAGTTCAAGTAATAAACTAGTTTCAAAAATAAATTTA
AATTCTTTAATTTTAATTTCATTTTCAACAATAATCACATTTGGATTATT
TACAAAAAACAATCCAAGATCTTCATCAAAACCATTATTATTTATTGTAT
TATTAATTTGTATTAGTATTTTTAATTTATCAAATTCAATTAAAATATCA
GAGGGTTTCAGTTCGTATTGATATTAAAGTACCATCAACATTTAAATTTG
ACTCATTGATATTGACATTGGNNNNNNNNN----------AACCAGCACT
CGTTGGTTCACATGAACTATGGTATAAATTTGATAACACTTGTGAATATA
ATTCCGATTGGACTTGTCATATTTGTGAAAAAGGTAATAAAGAAATTGCT
AATATTCAGTTTATAATTCCAGGTTTAATTGATGATGGTGTTTTAGTTTA
TGATTCATTTGAAAATCTTAATGTTGGTAACATTAGTTTATTTGGAAGTG
GTATCACTGAAAGAAGAAGTACTATTGTAACAAAAAATCAAGGTGTAACT
GGTGTTAGTAATATGGGTTGGTATCTATATTTAAAGGCTGGTAGCCCAAA
ATATATGAAAATTTGGTTATCACAAGTTGTATATGGTCAACATATATTTT
TAGCTCTTCCTTACCCAACACAAACAACTTTTATTATTAAATCAAATTAC
ATTTGGGGTAGTGCAATTAATTATAACCATGTTTTTTCACTTGCAAGTAG
TGCAGCTGATGTCAGATCTGGTAATGGTACAAAATACTATTTTGATGGTA
ATTTCCTATATGTTAAACTTGTTAATTTGGTGCTAACGGGTGATCCATCA
GAATACTATGAGAGAGATGGTGTTAAAGTATATTGGGTTAGAAATAGTTT
CTCAGTTTCTATTGAAGCTACAAATACTGTTACTCCACCAACATCTGACG
GTTTCTTTAAAAATCTTCCAGATAATTTACCTTCCAGTTCTTTATAAACC
TATTTTTAATAAAAAAAAACCTATGTATTTTTAAC
Gap gap included
Contig length 1325
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 1423030
End point 1421714
Strand (PLUS/MINUS) MINUS
Number of clones 7
Number of EST 8
Link to clone list U12069
List of clone(s) 

est1=SHE203F,1,571
est2=VHL419Z,572,1273
est3=VHJ279Z,574,1301
est4=VHF723Z,599,1297
est5=VHF677Z,603,1317
est6=VHH101Z,683,1307
est7=CHM751Z,703,1306
est8=SHE203Z,944,1273
Translated Amino Acid sequence 
klliyilk*k*iklfyffsyhvyiyfplvyqhnnywekskllkkvlqsmhllliqklii*
*iiq*qnqnyqlhqylvkrqvlmiffe*vmiksl*hlvtiqvvhlvqvin*fqk*i*il*
f*fhfqq*shldylqktiqdlhqnhyyllyy*FVLVFLIYQIQLKYQRVSVRIDIKVPST
FKFDSLILTLXXX---

---PALVGSHELWYKFDNTCEYNSDWTCHICEKGNKEIANIQFIIPGLIDDGVLVYDSFE
NLNVGNISLFGSGITERRSTIVTKNQGVTGVSNMGWYLYLKAGSPKYMKIWLSQVVYGQH
IFLALPYPTQTTFIIKSNYIWGSAINYNHVFSLASSAADVRSGNGTKYYFDGNFLYVKLV
NLVLTGDPSEYYERDGVKVYWVRNSFSVSIEATNTVTPPTSDGFFKNLPDNLPSSSL*ty
f**kktyvfl
Translated Amino Acid sequence (All Frames) 
Frame A:
klliyilk*k*iklfyffsyhvyiyfplvyqhnnywekskllkkvlqsmhllliqklii*
*iiq*qnqnyqlhqylvkrqvlmiffe*vmiksl*hlvtiqvvhlvqvin*fqk*i*il*
f*fhfqq*shldylqktiqdlhqnhyyllyy*FVLVFLIYQIQLKYQRVSVRIDIKVPST
FKFDSLILTLXXX---

---nqhslvhmnyginlitlvniipiglvifvkkvikkllifsl*fqv*lmmvf*fmihl
kilmlvtlvylevvslkeevll*qkikv*lvlviwvgiyi*rlvaqni*kfgyhklymvn
iyf*lfltqhkqllllnqitfgvvqliitmffhlqvvqlmsdlvmvqntilmvisymlnl
liwc*rvihqntmremvlkyigleivsqfllklqilllhqhltvslkifqiiylpvlykp
ifnkkkpmyf*

Frame B:
ny*yiy*nenk*nyfifflimfififl*cisttiigrnrny*kkfynrcicy*ykn*lfn
r*ynnktkiisyiniw*sdky**yssnk***kvfniw*lyk*yt*fk**tsfknkfkffn
fnfifnnnhiwiiykkqskifiktiiiyciinly*yf*fikfn*nirgfqfvlilkyhqh
lnlth*y*hwxxx---

---tstrwft*tmv*i**hl*i*frldlsyl*kr**rnc*ysvynsrfn**wcfsl*fi*
ks*cw*h*fiwkwyh*kkkyycnkksrcnwc**yglvsifkgw*pkiyenlvitsciwst
yifssslpntnnfyy*iklhlg*cn*l*pcfftck*cs*cqiw*wykilf*w*fpic*tc
*fgang*siril*erwc*silg*k*flsfy*sykycystni*rfl*kssr*ftfqffinl
flikknlcifn

Frame C:
iiniyikmkinkiilffflsclylfsssvsaqqllgeieiteksftidasvintkinyli
dntitkpklsvtsifgeatsiddilrisddkksltfgdytsstlsssnklvskinlnsli
lisfstiitfglftknnprssskpllfivllicisifnlsnsikisegfssy*y*stini
*i*lididigxx---

---PALVGSHELWYKFDNTCEYNSDWTCHICEKGNKEIANIQFIIPGLIDDGVLVYDSFE
NLNVGNISLFGSGITERRSTIVTKNQGVTGVSNMGWYLYLKAGSPKYMKIWLSQVVYGQH
IFLALPYPTQTTFIIKSNYIWGSAINYNHVFSLASSAADVRSGNGTKYYFDGNFLYVKLV
NLVLTGDPSEYYERDGVKVYWVRNSFSVSIEATNTVTPPTSDGFFKNLPDNLPSSSL*ty
f**kktyvfl
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U12069-1 (Contig-U12069-1Q)
/CSM_Contig/Contig-U12069-1Q.Seq.d
         (1335 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U12069-1 (Contig-U12069-1Q) /CSM_Contig/Conti...  1427  0.0
Contig-U10908-1 (Contig-U10908-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U12543-1 (Contig-U12543-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U02469-1 (Contig-U02469-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U14053-1 (Contig-U14053-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U12079-1 (Contig-U12079-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U11896-1 (Contig-U11896-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U11245-1 (Contig-U11245-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U09796-1 (Contig-U09796-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U08154-1 (Contig-U08154-1Q) /CSM_Contig/Conti...    38  0.024

>Contig-U12069-1 (Contig-U12069-1Q) /CSM_Contig/Contig-U12069-1Q.Seq.d
          Length = 1335

 Score = 1427 bits (720), Expect = 0.0
 Identities = 720/720 (100%)
 Strand = Plus / Plus

                                                                        
Query: 591  aaccagcactcgttggttcacatgaactatggtataaatttgataacacttgtgaatata 650
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 591  aaccagcactcgttggttcacatgaactatggtataaatttgataacacttgtgaatata 650

                                                                        
Query: 651  attccgattggacttgtcatatttgtgaaaaaggtaataaagaaattgctaatattcagt 710
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 651  attccgattggacttgtcatatttgtgaaaaaggtaataaagaaattgctaatattcagt 710

                                                                        
Query: 711  ttataattccaggtttaattgatgatggtgttttagtttatgattcatttgaaaatctta 770
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 711  ttataattccaggtttaattgatgatggtgttttagtttatgattcatttgaaaatctta 770

                                                                        
Query: 771  atgttggtaacattagtttatttggaagtggtatcactgaaagaagaagtactattgtaa 830
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 771  atgttggtaacattagtttatttggaagtggtatcactgaaagaagaagtactattgtaa 830

                                                                        
Query: 831  caaaaaatcaaggtgtaactggtgttagtaatatgggttggtatctatatttaaaggctg 890
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 831  caaaaaatcaaggtgtaactggtgttagtaatatgggttggtatctatatttaaaggctg 890

                                                                        
Query: 891  gtagcccaaaatatatgaaaatttggttatcacaagttgtatatggtcaacatatatttt 950
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 891  gtagcccaaaatatatgaaaatttggttatcacaagttgtatatggtcaacatatatttt 950

                                                                        
Query: 951  tagctcttccttacccaacacaaacaacttttattattaaatcaaattacatttggggta 1010
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 951  tagctcttccttacccaacacaaacaacttttattattaaatcaaattacatttggggta 1010

                                                                        
Query: 1011 gtgcaattaattataaccatgttttttcacttgcaagtagtgcagctgatgtcagatctg 1070
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1011 gtgcaattaattataaccatgttttttcacttgcaagtagtgcagctgatgtcagatctg 1070

                                                                        
Query: 1071 gtaatggtacaaaatactattttgatggtaatttcctatatgttaaacttgttaatttgg 1130
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1071 gtaatggtacaaaatactattttgatggtaatttcctatatgttaaacttgttaatttgg 1130

                                                                        
Query: 1131 tgctaacgggtgatccatcagaatactatgagagagatggtgttaaagtatattgggtta 1190
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1131 tgctaacgggtgatccatcagaatactatgagagagatggtgttaaagtatattgggtta 1190

                                                                        
Query: 1191 gaaatagtttctcagtttctattgaagctacaaatactgttactccaccaacatctgacg 1250
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1191 gaaatagtttctcagtttctattgaagctacaaatactgttactccaccaacatctgacg 1250

                                                                        
Query: 1251 gtttctttaaaaatcttccagataatttaccttccagttctttataaacctatttttaat 1310
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1251 gtttctttaaaaatcttccagataatttaccttccagttctttataaacctatttttaat 1310


 Score =  809 bits (408), Expect = 0.0
 Identities = 424/432 (98%)
 Strand = Plus / Plus

                                                                       
Query: 1   aaattattaatatatatattaaaatgaaaataaataaaattattttannnnnnnncttat 60
           |||||||||||||||||||||||||||||||||||||||||||||||        |||||
Sbjct: 1   aaattattaatatatatattaaaatgaaaataaataaaattattttattttttttcttat 60

                                                                       
Query: 61  catgtttatatttattttcctctagtgtatcagcacaacaattattgggagaaatcgaaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  catgtttatatttattttcctctagtgtatcagcacaacaattattgggagaaatcgaaa 120

                                                                       
Query: 121 ttactgaaaaaagttttacaatcgatgcatctgttattaatacaaaaattaattatttaa 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 ttactgaaaaaagttttacaatcgatgcatctgttattaatacaaaaattaattatttaa 180

                                                                       
Query: 181 tagataatacaataacaaaaccaaaattatcagttacatcaatatttggtgaagcgacaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 tagataatacaataacaaaaccaaaattatcagttacatcaatatttggtgaagcgacaa 240

                                                                       
Query: 241 gtattgatgatattcttcgaataagtgatgataaaaagtctttaacatttggtgactata 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gtattgatgatattcttcgaataagtgatgataaaaagtctttaacatttggtgactata 300

                                                                       
Query: 301 caagtagtacacttagttcaagtaataaactagtttcaaaaataaatttaaattctttaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 caagtagtacacttagttcaagtaataaactagtttcaaaaataaatttaaattctttaa 360

                                                                       
Query: 361 ttttaatttcattttcaacaataatcacatttggattatttacaaaaaacaatccaagat 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 ttttaatttcattttcaacaataatcacatttggattatttacaaaaaacaatccaagat 420

                       
Query: 421 cttcatcaaaac 432
           ||||||||||||
Sbjct: 421 cttcatcaaaac 432


 Score =  145 bits (73), Expect = 1e-34
 Identities = 73/73 (100%)
 Strand = Plus / Plus

                                                                       
Query: 499 cagagggtttcagttcgtattgatattaaagtaccatcaacatttaaatttgactcattg 558
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 499 cagagggtttcagttcgtattgatattaaagtaccatcaacatttaaatttgactcattg 558

                        
Query: 559 atattgacattgg 571
           |||||||||||||
Sbjct: 559 atattgacattgg 571


 Score = 32.2 bits (16), Expect = 1.5
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                          
Query: 7  ttaatatatatattaa 22
          ||||||||||||||||
Sbjct: 22 ttaatatatatattaa 7


>Contig-U10908-1 (Contig-U10908-1Q) /CSM_Contig/Contig-U10908-1Q.Seq.d
          Length = 1907

 Score = 42.1 bits (21), Expect = 0.002
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                 
Query: 359  aattttaatttcattttcaac 379
            |||||||||||||||||||||
Sbjct: 1848 aattttaatttcattttcaac 1868


>Contig-U12543-1 (Contig-U12543-1Q) /CSM_Contig/Contig-U12543-1Q.Seq.d
          Length = 1426

 Score = 40.1 bits (20), Expect = 0.006
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 524 ttaaagtaccatcaacattt 543
           ||||||||||||||||||||
Sbjct: 62  ttaaagtaccatcaacattt 43


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 30,556
Number of Sequences: 6905
Number of extensions: 30556
Number of successful extensions: 4115
Number of sequences better than 10.0: 534
length of query: 1335
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1319
effective length of database: 5,564,391
effective search space: 7339431729
effective search space used: 7339431729
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.29
Homology vs DNA 
Query= Contig-U12069-1 (Contig-U12069-1Q) /CSM_Contig/Contig-U12069-1Q.Seq.d
         (1335 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442452) Dictyostelium discoideum cDNA clone:ddv49m20, 3' ...  1417   0.0    1
(BJ440168) Dictyostelium discoideum cDNA clone:ddv42i20, 3' ...  1360   0.0    2
(BJ443486) Dictyostelium discoideum cDNA clone:ddv53f06, 3' ...  1356   0.0    2
(BJ439958) Dictyostelium discoideum cDNA clone:ddv42n05, 3' ...  1344   0.0    1
(BJ440867) Dictyostelium discoideum cDNA clone:ddv45a01, 3' ...  1201   0.0    1
(BJ383882) Dictyostelium discoideum cDNA clone:ddc47f13, 3' ...  1168   0.0    1
(BJ393631) Dictyostelium discoideum cDNA clone:dds32e02, 5' ...   529   0.0    3
(BJ405542) Dictyostelium discoideum cDNA clone:dds32e02, 3' ...   274   e-140  6
(ET762940) CHO_OF123xp22f1.ab1 CHO_OF Nicotiana tabacum geno...    52   0.055  1
(CX587320) TTE00035409 Amplicon Express - Conjugative Form T...    52   0.055  1
(CX573229) TTE00020841 Amplicon Express - Conjugative Form T...    52   0.055  1
(FF573816) TTEG409THB Tetrahymena thermophila conjugation cD...    52   0.055  1
(FF571188) TTEBK78THC Tetrahymena thermophila conjugation cD...    52   0.055  1
(U23453) Caenorhabditis elegans cosmid B0252, complete seque...    42   0.081  4
(CR940353) Theileria annulata genomic DNA chromosome 4.            38   0.42   2
(BJ441920) Dictyostelium discoideum cDNA clone:ddv48c09, 3' ...    38   0.45   3
(BJ442521) Dictyostelium discoideum cDNA clone:ddv50k05, 3' ...    38   0.55   3
(CW719110) MARC_1072973 CHORI-240-65I1 Bos taurus genomic cl...    40   0.71   2
(CW729533) MARC_763745 CHORI-240-65I1 Bos taurus genomic clo...    40   0.86   2
(AF063866) Melanoplus sanguinipes entomopoxvirus, complete g...    48   0.86   1
(AC094784) Rattus norvegicus 11 BAC CH230-4O6 (Children's Ho...    48   0.86   1
(AL157705) Human DNA sequence from clone RP11-148C9 on chrom...    48   0.86   1
(AC134718) Rattus norvegicus clone CH230-318O18, WORKING DRA...    48   0.86   1
(AC121712) Rattus norvegicus clone CH230-166K5, *** SEQUENCI...    48   0.86   1
(EK396375) 1095469522585 Global-Ocean-Sampling_GS-31-01-01-1...    46   0.91   2
(CW718970) MARC_1072736 CHORI-240-99I17 Bos taurus genomic c...    40   0.97   2
(FF847501) EST_tthy_evl_878742 tthy_evl Thymallus thymallus ...    36   1.00   2
(AC178097) Strongylocentrotus purpuratus clone R3-1117D16, W...    46   1.0    4
(FF847500) EST_tthy_evl_877206 tthy_evl Thymallus thymallus ...    36   1.0    2
(CW719019) MARC_1072812 CHORI-240-65I1 Bos taurus genomic cl...    40   1.1    2
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    38   1.1    15
(CP000743) Methanococcus aeolicus Nankai-3, complete genome.       32   1.7    19
(EJ705906) 1092956053523 Global-Ocean-Sampling_GS-30-02-01-1...    34   1.8    3
(AM285311) Spiroplasma citri GII3-3X chromosome, contig Cont...    32   1.9    6
(CP000123) Mycoplasma capricolum subsp. capricolum ATCC 2734...    32   2.9    20
(AX458660) Sequence 206 from Patent WO0246454.                     36   3.1    2
(CW729602) MARC_763821 CHORI-240-65I1 Bos taurus genomic clo...    40   3.4    2
(BX255955) Zebrafish DNA sequence from clone CH211-249G22 in...    46   3.4    1
(BC095806) Danio rerio zgc:112407, mRNA (cDNA clone MGC:1124...    46   3.4    1
(AL929264) Zebrafish DNA sequence from clone CH211-59C24 in ...    46   3.4    1
(BX883048) Rattus norvegicus chromosome 20, major histocompa...    46   3.4    1
(AL773518) Mouse DNA sequence from clone RP24-231F12 on chro...    46   3.4    1
(AE001396) Plasmodium falciparum 3D7 chromosome 2 section 33...    46   3.4    1
(AC124146) Rattus norvegicus clone CH230-122E10, WORKING DRA...    46   3.4    1
(AC104401) Rattus norvegicus clone CH230-132A21, *** SEQUENC...    46   3.4    1
(AC099458) Rattus norvegicus clone CH230-216O20, WORKING DRA...    46   3.4    1
(AL603726) Rattus norvegicus chromosome 20 clone PAC RPCI-31...    46   3.4    1
(AC179944) Strongylocentrotus purpuratus clone R3-3032O19, W...    46   3.4    1
(AZ526177) 252PbE10 Pb MBN #21 Plasmodium berghei genomic 3'...    46   3.4    1
(FI021501) CHO_OF6929xe13r1.ab1 CHO_OF6 Nicotiana tabacum ge...    46   3.4    1
(FI021423) CHO_OF6929xe13f1.ab1 CHO_OF6 Nicotiana tabacum ge...    46   3.4    1
(EK254703) 1095462005419 Global-Ocean-Sampling_GS-31-01-01-1...    46   3.4    1
(EJ693450) 1092956010904 Global-Ocean-Sampling_GS-30-02-01-1...    46   3.4    1
(EJ666955) 1092955044958 Global-Ocean-Sampling_GS-30-02-01-1...    46   3.4    1
(CZ418881) 1014584 RP42 Bos taurus genomic clone RP42-21B12,...    46   3.4    1
(EL574883) Physarum07764 Physarum polycephalum starvation st...    46   3.4    1
(EL574307) Physarum07765 Physarum polycephalum starvation st...    46   3.4    1
(EL572437) Physarum07762 Physarum polycephalum starvation st...    46   3.4    1
(EL571609) Physarum07761 Physarum polycephalum starvation st...    46   3.4    1
(EL569931) Physarum02028 Physarum polycephalum starvation st...    46   3.4    1
(EL566974) Physarum07763 Physarum polycephalum starvation st...    46   3.4    1
(EL566247) Physarum07760 Physarum polycephalum starvation st...    46   3.4    1
(EG673350) RCRAA54TP Castor bean cDNA library from roots, 1....    46   3.4    1
(EC762906) PPE00008673 Agencourt Biosciences Agen-0020 Non-n...    46   3.4    1
(EC755758) PPE00005407 Agencourt Biosciences Agen-0020 Non-n...    46   3.4    1
(EC753456) PPE00009147 Agencourt Biosciences Agen-0020 Non-n...    46   3.4    1
(DC881595) Ipomoea batatas mRNA, clone: IT1230, 3' end seque...    46   3.4    1
(CD295354) StrPu691.006749 Sea urchin larva cDNA library MPM...    46   3.4    1
(CD294805) StrPu691.006200 Sea urchin larva cDNA library MPM...    46   3.4    1
(AI547730) UI-R-C3-sj-b-02-0-UI.s1 UI-R-C3 Rattus norvegicus...    46   3.4    1
(BQ451498) PfESToab01b06.y1 Plasmodium falciparum 3D7 asexua...    46   3.4    1
(BI814912) PfESToaa07h10.y1 Plasmodium falciparum 3D7 asexua...    46   3.4    1
(BI670796) PfESToaa04d10.y1 Plasmodium falciparum 3D7 asexua...    46   3.4    1
(AL390863) Human DNA sequence from clone RP11-239J11 on chro...    34   4.6    7
(AC136583) Rattus norvegicus clone CH230-4H16, *** SEQUENCIN...    34   6.2    2
(AC176814) Strongylocentrotus purpuratus clone R3-3111B06, W...    38   6.2    5
(AX347077) Sequence 2148 from Patent WO0200928.                    42   6.5    6
(CS374432) Sequence 535 from Patent WO2006034879.                  36   6.6    2
(CS374160) Sequence 263 from Patent WO2006034879.                  36   6.6    2
(CU463336) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    34   6.6    7
(AE017308) Mycoplasma mobile 163K complete genome.                 38   6.6    16
(EA102667) Sequence 50 from patent US 7195870.                     36   7.1    2
(DD209291) test.                                                   36   7.1    2
(DD209163) test.                                                   36   7.1    2
(AX826128) Sequence 380 from Patent WO03072821.                    36   7.1    2
(AX826000) Sequence 252 from Patent WO03072821.                    36   7.1    2
(AX822488) Sequence 380 from Patent EP1340818.                     36   7.1    2
(AX822360) Sequence 252 from Patent EP1340818.                     36   7.1    2
(AX795958) Sequence 301 from Patent WO03052135.                    36   7.1    2
(AX795842) Sequence 185 from Patent WO03052135.                    36   7.1    2
(AX767556) Sequence 204 from Patent WO03044226.                    36   7.1    2
(AX767480) Sequence 128 from Patent WO03044226.                    36   7.1    2
(AX345573) Sequence 644 from Patent WO0200928.                     36   7.1    2
(AX323562) Sequence 50 from Patent WO0192565.                      36   7.1    2
(AX277887) Sequence 50 from Patent WO0177375.                      36   7.1    2
(AC178903) Strongylocentrotus purpuratus clone R3-3114N5, WO...    38   7.2    6
(DJ060330) Method and nucleic acids for the improved treatme...    36   7.3    2
(DJ060208) Method and nucleic acids for the improved treatme...    36   7.3    2
(DD392082) Method and nucleic acids for the improved treatme...    36   7.3    2
(DD391808) Method and nucleic acids for the improved treatme...    36   7.3    2

>(BJ442452) Dictyostelium discoideum cDNA clone:ddv49m20, 3' end,
            single read.
          Length = 728

 Score = 1417 bits (715), Expect = 0.0
 Identities = 717/718 (99%)
 Strand = Plus / Minus


Query: 593  ccagcactcgttggttcacatgaactatggtataaatttgataacacttgtgaatataat 652
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 728  ccagcactcgttggttcacatgaactatggtataaatttgataacacttgtgaatataat 669


Query: 653  tccgattggacttgtcatatttgtgaaaaaggtaataaagaaattgctaatattcagttt 712
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 668  tccgattggacttgtcatatttgtgaaaaaggtaataaagaaattgctaatattcagttt 609


Query: 713  ataattccaggtttaattgatgatggtgttttagtttatgattcatttgaaaatcttaat 772
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 608  ataattccaggtttaattgatgatggtgttttagtttatgattcatttgaaaatcttaat 549


Query: 773  gttggtaacattagtttatttggaagtggtatcactgaaagaagaagtactattgtaaca 832
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 548  gttggtaacattagtttatttggaagtggtatcactgaaagaagaagtactattgtaaca 489


Query: 833  aaaaatcaaggtgtaactggtgttagtaatatgggttggtatctatatttaaaggctggt 892
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 488  aaaaatcaaggtgtaactggtgttagtaatatgggttggtatctatatttaaaggctggt 429


Query: 893  agcccaaaatatatgaaaatttggttatcacaagttgtatatggtcaacatatattttta 952
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 428  agcccaaaatatatgaaaatttggttatcacaagttgtatatggtcaacatatattttta 369


Query: 953  gctcttccttacccaacacaaacaacttttattattaaatcaaattacatttggggtagt 1012
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 368  gctcttccttacccaacacaaacaacttttattattaaatcaaattacatttggggtagt 309


Query: 1013 gcaattaattataaccatgttttttcacttgcaagtagtgcagctgatgtcagatctggt 1072
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 308  gcaattaattataaccatgttttttcacttgcaagtagtgcagctgatgtcagatctggt 249


Query: 1073 aatggtacaaaatactattttgatggtaatttcctatatgttaaacttgttaatttggtg 1132
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 248  aatggtacaaaatactattttgatggtaatttcctatatgttaaacttgttaatttggtg 189


Query: 1133 ctaacgggtgatccatcagaatactatgagagagatggtgttaaagtatattgggttaga 1192
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 188  ctaacgggtgatccatcagaatactatgagagagatggtgttaaagtatattgggttaga 129


Query: 1193 aatagtttctcagtttctattgaagctacaaatactgttactccaccaacatctgacggt 1252
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 128  aatagtttctcagtttctattgaagctacaaatactgttactccaccaacatctgacggt 69


Query: 1253 ttctttaaaaatcttccagataatttaccttccagttctttataaacctatttttaat 1310
            |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct: 68   ttctttaaaaatcttccagataatttaccttccagttctttataaaccnatttttaat 11

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,471,438,465
Number of extensions: 91961998
Number of successful extensions: 8508990
Number of sequences better than 10.0: 116
Length of query: 1335
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1311
Effective length of database: 97,308,875,965
Effective search space: 127571936390115
Effective search space used: 127571936390115
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 2
Homology vs Protein 
Query= Contig-U12069-1 (Contig-U12069-1Q) /CSM_Contig/Contig-U12069-1Q.Seq.d
         (1335 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_193(AC116984|pid:none) Dictyostelium discoideum chromos...   226   2e-57
CP001576_63(CP001576|pid:none) Micromonas sp. RCC299 chromosome ...    49   3e-04
AB027295_5(AB027295|pid:none) Physarum polycephalum mitochondria...    36   2.8
CP001539_15(CP001539|pid:none) Borrelia burgdorferi 118a plasmid...    35   6.1
AE001437_1066(AE001437|pid:none) Clostridium acetobutylicum ATCC...    35   6.1

>AC116984_193(AC116984|pid:none) Dictyostelium discoideum chromosome 2
            map 2567470-3108875 strain AX4, complete sequence.
          Length = 1096

 Score =  226 bits (575), Expect = 2e-57
 Identities = 106/234 (45%), Positives = 160/234 (68%)
 Frame = +2

Query: 593  PALVGSHELWYKFDNTCEYNSDWTCHICEKGNKEIANIQFIIPGLIDDGVLVYDSFENLN 772
            P +VGSH  W+KFD++C ++++W   +C KG K +ANI+F +PG ++  +   +   +
Sbjct: 869  PQIVGSHVSWWKFDDSCLFSTEWNVWVCNKGTKGLANIEFWVPGFMEREL---EQDPDSY 925

Query: 773  VGNISLFGSGITERRSTIVTKNQGVTGVSNMGWYLYLKAGSPKYMKIWLSQVVYGQHIFL 952
            +G+ISLFGSGI + R T++T+N G+TGVSNMGWY Y   G+P Y++IW +Q+ +G++IFL
Sbjct: 926  IGSISLFGSGINDERKTLLTRNPGITGVSNMGWYAYFTIGTPNYLRIWTAQIAFGEYIFL 985

Query: 953  ALPYPTQTTFIIKSNYIWGSAINYNHVFSLASSAADVRSGNGTKYYFDGNFLYVKLVNLV 1132
            A+PYPT TTF I S Y + +   Y +  +  +SA  V+ GNG +YYFDG  L++KLVN +
Sbjct: 986  AIPYPTGTTFTITSEYDYSN--QYTYTITKTTSALAVKQGNGKQYYFDGTHLFLKLVNFM 1043

Query: 1133 LTGDPSEYYERDGVKVYWVRNSFSVSIEATNTVTPPTSDGFFKNLPDNLPSSSL 1294
             TG   E ++R G+K+  V  ++  +I ATNT   P+ +G+F NLPD  PSS+L
Sbjct: 1044 NTGGSWESFDRVGIKIPDVYWTYIYNIRATNT-AKPSVNGYFLNLPDVRPSSTL 1096

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,732,666,323
Number of extensions: 33679503
Number of successful extensions: 89789
Number of sequences better than 10.0: 5
Number of HSP's gapped: 89766
Number of HSP's successfully gapped: 5
Length of query: 445
Length of database: 1,051,180,864
Length adjustment: 132
Effective length of query: 313
Effective length of database: 623,955,076
Effective search space: 195297938788
Effective search space used: 195297938788
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.97  gvh: 0.68  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for Contig-U12069-1 is exc
VS (DIR, S) 0
VH (FL, L) 5
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 1
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0