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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U11943-1
Contig ID Contig-U11943-1
Contig update 2002.12.18
Contig sequence 
>Contig-U11943-1 (Contig-U11943-1Q) /CSM_Contig/Contig-U11943-1Q.Seq.d
ATTTTTGTTTTTTTTATTTGTATTTATTTTTTATATAGGGAAATAATTTT
TTGTCCTTATTTTAATTTAATTACCATTATATATATATTTATTTAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNAAAAAAAAAAATTTT
TTTTTTTTTNAAAAAAAANTTTTNAAAAAAAAAAAAAAAAAANNNAAAAA
NTTTTAAAAAAAANNNNNAATTTTTTTTAAAAAAAAAAAAAAAAANTGGG
GGGGGGNANNNAAANTTTTTNAAAAAANNNCCCCCNNCNGGGGTTTTTTT
TTTTTTTTTTNAAAANCNTTTTCNNNNCNNNNAAAAAANNNNNNNNN---
-------NNNNNNNNNNCAATTCAGAGTATAGTTTCANCANCAANTNCAN
CAACCCCAACAACCACAAGTACAGCTGGCACAAATAATAGTAATACAACC
AATAATAGTATAAAACCACCAGCAACTCAATTTAGTATTTATGATTTTGT
AAAACCACAATCACAACCAGAATTAAGTGGTAATAAAAAACAATCATACC
ATCAAAAGATACTATTTACATCATCACCAGCAGCTAATTCAATATCTCAA
AATATTTCACCAATGATAACTCCAATATCTTCACCAATCGCTCCAATTTT
AAAAAATAGAAATAATTTTAATAATATGGGTAATGGAAATAAAATTTCAA
AATTATTAATATCTGAAAATAATAATAATTCACAAAATGTTACACCAAAA
TTGAAATCATTTAAAAATAAATTGGATAGTGAATTATTACAATCATTAGA
AGCATTAATTGATTCACCACAAAATGTATCAAACTCTAATATTGATAATT
GTTTTGGTACTGATTCTCCTAGATTATTATTA
Gap gap included
Contig length 872
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 1
Number of EST 2
Link to clone list U11943
List of clone(s) 

est1=CHR811F,1,338
est2=CHR811Z,339,854
Translated Amino Acid sequence 
flfflfvfifyigk*ffvlili*LPLYIYLFKKKKKKKKKKKKKXKKKIFFFXKKXFXKK
KKKXXKXFKKXXXFFLKKKKKXGGGXXXFXKKXPPXGVFFFFFXXXFXXXXKXXX---

---XXXXIQSIVSXXXXXTPTTTSTAGTNNSNTTNNSIKPPATQFSIYDFVKPQSQPELS
GNKKQSYHQKILFTSSPAANSISQNISPMITPISSPIAPILKNRNNFNNMGNGNKISKLL
ISENNNNSQNVTPKLKSFKNKLDSELLQSLEALIDSPQNVSNSNIDNCFGTDSPRLLL
Translated Amino Acid sequence (All Frames) 
Frame A:
ifvfficiyflyreiifcpyfnlitiiyifi*kkkkkkkkkkkkxkkknffffxkkxxkk
kkkkxkxf*kkxxiffkkkkkxwggxxkxfxkxxpxxgfffffxkxfsxxxkxxx---

---XXXXIQSIVSXXXXXTPTTTSTAGTNNSNTTNNSIKPPATQFSIYDFVKPQSQPELS
GNKKQSYHQKILFTSSPAANSISQNISPMITPISSPIAPILKNRNNFNNMGNGNKISKLL
ISENNNNSQNVTPKLKSFKNKLDSELLQSLEALIDSPQNVSNSNIDNCFGTDSPRLLL

Frame B:
flfflfvfifyigk*ffvlili*LPLYIYLFKKKKKKKKKKKKKXKKKIFFFXKKXFXKK
KKKXXKXFKKXXXFFLKKKKKXGGGXXXFXKKXPPXGVFFFFFXXXFXXXXKXXX---

---xxxqfrv*fxxqxxqpqqpqvqlaqiiviqpiiv*nhqqlnlvfmil*nhnhnqn*v
viknnhtikryylhhhqqliqylkifhq**lqylhqslqf*kieiiliiwvmeikfqny*
ylkiiiihkmlhqn*nhlkinwivnyynh*kh*lihhkmyqtliliivlvlilldyy

Frame C:
fcffylylffi*gnnflslf*fnyhyiyiylkkkkkkkkkkkkxxkkkffffxkkxfxkk
kkkxxkxlkkxxnff*kkkkkxgggxxxfxkxxpxxgfffffxkxxfxxxkkxxx---

---xxxnseysfxxxxxnpnnhkyswhk***ynq**ykttsnsi*yl*fckttittrikw
**ktiipskdtiyiitss*fniskyftndnsniftnrsnfkk*k*f**yg*wk*nfkiin
i*k***ftkcytkieii*k*ig**iitiirsin*fttkcikl*y**lfwy*fs*iii
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U11943-1 (Contig-U11943-1Q)
/CSM_Contig/Contig-U11943-1Q.Seq.d
         (882 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U11943-1 (Contig-U11943-1Q) /CSM_Contig/Conti...   547  e-156
Contig-U13400-1 (Contig-U13400-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U13314-1 (Contig-U13314-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U09157-1 (Contig-U09157-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U13844-1 (Contig-U13844-1Q) /CSM_Contig/Conti...    34  0.25
Contig-U12090-1 (Contig-U12090-1Q) /CSM_Contig/Conti...    34  0.25
Contig-U11332-1 (Contig-U11332-1Q) /CSM_Contig/Conti...    34  0.25
Contig-U11251-1 (Contig-U11251-1Q) /CSM_Contig/Conti...    34  0.25
Contig-U10968-1 (Contig-U10968-1Q) /CSM_Contig/Conti...    34  0.25
Contig-U08778-1 (Contig-U08778-1Q) /CSM_Contig/Conti...    34  0.25

>Contig-U11943-1 (Contig-U11943-1Q) /CSM_Contig/Contig-U11943-1Q.Seq.d
          Length = 882

 Score =  547 bits (276), Expect = e-156
 Identities = 291/291 (100%)
 Strand = Plus / Plus

                                                                       
Query: 368 caattcagagtatagtttcancancaantncancaaccccaacaaccacaagtacagctg 427
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 368 caattcagagtatagtttcancancaantncancaaccccaacaaccacaagtacagctg 427

                                                                       
Query: 428 gcacaaataatagtaatacaaccaataatagtataaaaccaccagcaactcaatttagta 487
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 428 gcacaaataatagtaatacaaccaataatagtataaaaccaccagcaactcaatttagta 487

                                                                       
Query: 488 tttatgattttgtaaaaccacaatcacaaccagaattaagtggtaataaaaaacaatcat 547
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 488 tttatgattttgtaaaaccacaatcacaaccagaattaagtggtaataaaaaacaatcat 547

                                                                       
Query: 548 accatcaaaagatactatttacatcatcaccagcagctaattcaatatctcaaaatattt 607
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 548 accatcaaaagatactatttacatcatcaccagcagctaattcaatatctcaaaatattt 607

                                                              
Query: 608 caccaatgataactccaatatcttcaccaatcgctccaattttaaaaaata 658
           |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 608 caccaatgataactccaatatcttcaccaatcgctccaattttaaaaaata 658


 Score =  317 bits (160), Expect = 1e-86
 Identities = 160/160 (100%)
 Strand = Plus / Plus

                                                                       
Query: 723 taataattcacaaaatgttacaccaaaattgaaatcatttaaaaataaattggatagtga 782
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 723 taataattcacaaaatgttacaccaaaattgaaatcatttaaaaataaattggatagtga 782

                                                                       
Query: 783 attattacaatcattagaagcattaattgattcaccacaaaatgtatcaaactctaatat 842
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 783 attattacaatcattagaagcattaattgattcaccacaaaatgtatcaaactctaatat 842

                                                   
Query: 843 tgataattgttttggtactgattctcctagattattatta 882
           ||||||||||||||||||||||||||||||||||||||||
Sbjct: 843 tgataattgttttggtactgattctcctagattattatta 882


>Contig-U13400-1 (Contig-U13400-1Q) /CSM_Contig/Contig-U13400-1Q.Seq.d
          Length = 617

 Score = 40.1 bits (20), Expect = 0.004
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 754 aaatcatttaaaaataaatt 773
           ||||||||||||||||||||
Sbjct: 512 aaatcatttaaaaataaatt 493


>Contig-U13314-1 (Contig-U13314-1Q) /CSM_Contig/Contig-U13314-1Q.Seq.d
          Length = 627

 Score = 38.2 bits (19), Expect = 0.016
 Identities = 28/31 (90%)
 Strand = Plus / Plus

                                          
Query: 430 acaaataatagtaatacaaccaataatagta 460
           ||||||||||||||||| ||||  |||||||
Sbjct: 113 acaaataatagtaataccaccaccaatagta 143


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 11,712
Number of Sequences: 6905
Number of extensions: 11712
Number of successful extensions: 1675
Number of sequences better than 10.0: 142
length of query: 882
length of database: 5,674,871
effective HSP length: 16
effective length of query: 866
effective length of database: 5,564,391
effective search space: 4818762606
effective search space used: 4818762606
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.27
Homology vs DNA 
Query= Contig-U11943-1 (Contig-U11943-1Q) /CSM_Contig/Contig-U11943-1Q.Seq.d
         (882 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ386070) Dictyostelium discoideum cDNA clone:ddc57f04, 3' ...   519   0.0    2
(DU877236) 220188 Tomato HindIII BAC Library Solanum lycoper...    50   0.14   1
(AC115598) Dictyostelium discoideum chromosome 2 map 581427-...    32   1.1    9
(AL445649) Human DNA sequence from clone RP11-381O6 on chrom...    46   2.2    1
(AC183254) Bos taurus clone CH240-97D22, WORKING DRAFT SEQUE...    46   2.2    1
(AC023433) Homo sapiens clone RP11-114K22, WORKING DRAFT SEQ...    38   4.9    3
(BH599041) BOHET69TR BOHE Brassica oleracea genomic clone BO...    32   5.7    2
(BH686830) BOHTV73TF BO_2_3_KB Brassica oleracea genomic clo...    32   5.8    2
(AF111942) Dictyostelium discoideum AX4 development protein ...    36   6.1    2
(U84865) HIV-1 strain M15 from USA, envelope glycoprotein (e...    44   8.8    1
(CU041392) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   8.8    1
(BH432989) BOHHQ83TF BOHH Brassica oleracea genomic clone BO...    44   8.8    1
(FH968017) CHO_OF6785xf05f1.ab1 CHO_OF6 Nicotiana tabacum ge...    44   8.8    1
(FH446441) CHO_OF4702xg01f1.ab1 CHO_OF4 Nicotiana tabacum ge...    44   8.8    1
(FH426314) CHO_OF4905xh17r1.ab1 CHO_OF4 Nicotiana tabacum ge...    44   8.8    1
(ER314394) 1092344126372 Global-Ocean-Sampling_GS-34-01-01-1...    44   8.8    1
(EJ934909) 1093018831288 Global-Ocean-Sampling_GS-30-02-01-1...    44   8.8    1
(EJ930132) 1093018801960 Global-Ocean-Sampling_GS-30-02-01-1...    44   8.8    1
(EJ695550) 1092956017306 Global-Ocean-Sampling_GS-30-02-01-1...    44   8.8    1
(EJ675230) 1092955075760 Global-Ocean-Sampling_GS-30-02-01-1...    44   8.8    1
(EJ142205) 1092343661704 Global-Ocean-Sampling_GS-27-01-01-1...    44   8.8    1
(EJ119855) 1092343367916 Global-Ocean-Sampling_GS-27-01-01-1...    44   8.8    1
(CG824470) SOYFC42TV LargeInsertSoybeanGenLib Glycine max ge...    44   8.8    1
(ES392145) MUS10-G12.y1d-s SHGC-MUS Mytilus californianus cD...    44   8.8    1
(ES388411) MUS09-P11.y1d-s SHGC-MUS Mytilus californianus cD...    44   8.8    1
(BJ328249) Dictyostelium discoideum cDNA clone:dda23b13, 5' ...    44   8.8    1
(FK259668) USDA-FP_195631 Asian citrus psyllid midgut (Hemip...    44   8.8    1
(EY903393) RR2DW16JQ RR2(MS) Raphanus raphanistrum subsp. ra...    44   8.8    1

>(BJ386070) Dictyostelium discoideum cDNA clone:ddc57f04, 3' end,
           single read.
          Length = 516

 Score =  519 bits (262), Expect(2) = 0.0
 Identities = 277/277 (100%)
 Strand = Plus / Minus


Query: 368 caattcagagtatagtttcancancaantncancaaccccaacaaccacaagtacagctg 427
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 516 caattcagagtatagtttcancancaantncancaaccccaacaaccacaagtacagctg 457


Query: 428 gcacaaataatagtaatacaaccaataatagtataaaaccaccagcaactcaatttagta 487
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 456 gcacaaataatagtaatacaaccaataatagtataaaaccaccagcaactcaatttagta 397


Query: 488 tttatgattttgtaaaaccacaatcacaaccagaattaagtggtaataaaaaacaatcat 547
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 396 tttatgattttgtaaaaccacaatcacaaccagaattaagtggtaataaaaaacaatcat 337


Query: 548 accatcaaaagatactatttacatcatcaccagcagctaattcaatatctcaaaatattt 607
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 336 accatcaaaagatactatttacatcatcaccagcagctaattcaatatctcaaaatattt 277


Query: 608 caccaatgataactccaatatcttcaccaatcgctcc 644
           |||||||||||||||||||||||||||||||||||||
Sbjct: 276 caccaatgataactccaatatcttcaccaatcgctcc 240

 Score =  216 bits (109), Expect(2) = 0.0
 Identities = 109/109 (100%)
 Strand = Plus / Minus


Query: 774 ggatagtgaattattacaatcattagaagcattaattgattcaccacaaaatgtatcaaa 833
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 110 ggatagtgaattattacaatcattagaagcattaattgattcaccacaaaatgtatcaaa 51


Query: 834 ctctaatattgataattgttttggtactgattctcctagattattatta 882
           |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 50  ctctaatattgataattgttttggtactgattctcctagattattatta 2

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 450,998,883
Number of extensions: 28654067
Number of successful extensions: 2172990
Number of sequences better than 10.0: 28
Length of query: 882
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 858
Effective length of database: 97,308,875,965
Effective search space: 83491015577970
Effective search space used: 83491015577970
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7. 1
Homology vs Protein 
Query= Contig-U11943-1 (Contig-U11943-1Q) /CSM_Contig/Contig-U11943-1Q.Seq.d
         (882 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54673) RecName: Full=Phosphatidylinositol 3-kinase 1;         ...    35   2.6
AF091239_1(AF091239|pid:none) Plasmodium falciparum c-13 antigen...    29   9.2

>(P54673) RecName: Full=Phosphatidylinositol 3-kinase 1;
           Short=PtdIns-3-kinase;          Short=PI3-kinase;
             Short=PI3K;          EC=2.7.1.137;
          Length = 1571

 Score = 35.4 bits (80), Expect = 2.6
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = +1

Query: 712 SENNNNSQNVTPKLKSFKNKLDSELLQSLEALIDSPQNVSNSNIDNC 852
           ++NNNN+ N+  K K  KNK + E+ Q +       + + NSN  NC
Sbjct: 65  NDNNNNNNNIDKKRKDSKNKQNQEINQEM----SENKKIYNSNDSNC 107

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 713,301,845
Number of extensions: 15400503
Number of successful extensions: 25686
Number of sequences better than 10.0: 2
Number of HSP's gapped: 25554
Number of HSP's successfully gapped: 3
Length of query: 294
Length of database: 1,051,180,864
Length adjustment: 127
Effective length of query: 167
Effective length of database: 640,137,871
Effective search space: 106903024457
Effective search space used: 106903024457
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.03  gvh: 0.19  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 1.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for Contig-U11943-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 1
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0