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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U01737-1
Contig ID Contig-U01737-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U01737-1 (Contig-U01737-1Q) /CSM_Contig/Contig-U01737-1Q.Seq.d
ATTAAAAATAAATAAAAAAAAAAGTAATAATATAAAATAAAATATATATA
TATATATATATTTTAAATAAAAATGTTTTCACATTGTGAAGGATATAGAA
TTGAAGGTGGTGTAACTGATGGAGAAACAAATTGGAATAATAATTTCAAT
CAAAATAACAATAATAATGATAATAATAAAGTTATGGCATCAATATATAA
ANNNNNTTCTATGGTAAACCTCATAAAACGTTTATGAGTTTAAGTTCACC
TAATGGTCCAATTTTAGTTTGTATTAAAAAGGTTGGTAATTCATCAGATG
ATTTCTATTTATTAGTTTGTACTGAAAAAGGATTTGAAGAGGTAAGAGCA
AATAAAGATTCATTACAAAAATCAAAAACAACAAGATCATTCCTTAAAAT
GAGACCAACATCATTGAATGTTATTACAAGTGTCCGTCCAGAATTAACAA
AGGTACCTCTATGTAAAGTTACAGATAAATCAATTCAAGAAGAGTTAGTA
AAAGTTTGTGAACCAGAATTTAATAAAGTTATTAAATCTGCTCTACTCTA
TTGCAAAGAGTCTCAACGTGATGATAATGATATGCTTAAAAATATCAGCT
CAAATGTTTCTCAAGAATATAATGATTTTTTA
Gap no gap
Contig length 632
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 5565784
End point 5565205
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U01737
List of clone(s) 

est1=SSG137F,1,633
Translated Amino Acid sequence 
*k*ikkkvii*nkiyiyiyilnknvftl*ri*n*rwcn*wrnkle**fqsk*q******s
ygini*XXFYGKPHKTFMSLSSPNGPILVCIKKVGNSSDDFYLLVCTEKGFEEVRANKDS
LQKSKTTRSFLKMRPTSLNVITSVRPELTKVPLCKVTDKSIQEELVKVCEPEFNKVIKSA
LLYCKESQRDDNDMLKNISSNVSQEYNDFL
Translated Amino Acid sequence (All Frames) 
Frame A:
iknk*kkk**ykikyiyiyif*ikmfshcegyrieggvtdgetnwnnnfnqnnnnndnnk
vmasiykxxsmvnlikrl*v*vhlmvqf*fvlkrlvihqmisiy*fvlkkdlkr*eqiki
hyknqkqqdhslk*dqhh*mllqvsvqn*qrylyvklqinqfkks**kfvnqnlikllnl
lysiakslnvmimiclkisaqmflknimif


Frame B:
lkinkkksnnik*niyiyiyfk*kcfhivkdielkvv*lmekqigiiisikitiimiiik
lwhqyixxxlw*ts*nvyefkft*wsnfsly*kgw*fir*flfisly*kri*rgksk*rf
itkiknnkiip*netniiecyykcpsrinkgtsm*syr*insrrvsksl*tri**sy*ic
stllqrvst****ya*kyqlkcfsri**ff


Frame C:
*k*ikkkvii*nkiyiyiyilnknvftl*ri*n*rwcn*wrnkle**fqsk*q******s
ygini*XXFYGKPHKTFMSLSSPNGPILVCIKKVGNSSDDFYLLVCTEKGFEEVRANKDS
LQKSKTTRSFLKMRPTSLNVITSVRPELTKVPLCKVTDKSIQEELVKVCEPEFNKVIKSA
LLYCKESQRDDNDMLKNISSNVSQEYNDFL
own update 2004. 6. 7
Homology vs CSM-cDNA 
Query= Contig-U01737-1 (Contig-U01737-1Q)
/CSM_Contig/Contig-U01737-1Q.Seq.d
         (632 letters)

Database: CSM
           8361 sequences; 7,895,291 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U01737-1 (Contig-U01737-1Q) /CSM_Contig/Conti...   866  0.0
Contig-U11220-1 (Contig-U11220-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U09770-1 (Contig-U09770-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U13043-1 (Contig-U13043-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U10788-1 (Contig-U10788-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U10775-1 (Contig-U10775-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U07588-1 (Contig-U07588-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U03269-1 (Contig-U03269-1Q) /CSM_Contig/Conti...    36  0.062
Contig-U14380-1 (Contig-U14380-1Q) /CSM_Contig/Conti...    34  0.24
Contig-U14247-1 (Contig-U14247-1Q) /CSM_Contig/Conti...    34  0.24

>Contig-U01737-1 (Contig-U01737-1Q) /CSM_Contig/Contig-U01737-1Q.Seq.d
          Length = 632

 Score =  866 bits (437), Expect = 0.0
 Identities = 452/452 (100%)
 Strand = Plus / Plus

                                                                       
Query: 181 gttatggcatcaatatataaannnnnttctatggtaaacctcataaaacgtttatgagtt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 gttatggcatcaatatataaannnnnttctatggtaaacctcataaaacgtttatgagtt 240

                                                                       
Query: 241 taagttcacctaatggtccaattttagtttgtattaaaaaggttggtaattcatcagatg 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 taagttcacctaatggtccaattttagtttgtattaaaaaggttggtaattcatcagatg 300

                                                                       
Query: 301 atttctatttattagtttgtactgaaaaaggatttgaagaggtaagagcaaataaagatt 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 atttctatttattagtttgtactgaaaaaggatttgaagaggtaagagcaaataaagatt 360

                                                                       
Query: 361 cattacaaaaatcaaaaacaacaagatcattccttaaaatgagaccaacatcattgaatg 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 cattacaaaaatcaaaaacaacaagatcattccttaaaatgagaccaacatcattgaatg 420

                                                                       
Query: 421 ttattacaagtgtccgtccagaattaacaaaggtacctctatgtaaagttacagataaat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 ttattacaagtgtccgtccagaattaacaaaggtacctctatgtaaagttacagataaat 480

                                                                       
Query: 481 caattcaagaagagttagtaaaagtttgtgaaccagaatttaataaagttattaaatctg 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 caattcaagaagagttagtaaaagtttgtgaaccagaatttaataaagttattaaatctg 540

                                                                       
Query: 541 ctctactctattgcaaagagtctcaacgtgatgataatgatatgcttaaaaatatcagct 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ctctactctattgcaaagagtctcaacgtgatgataatgatatgcttaaaaatatcagct 600

                                           
Query: 601 caaatgtttctcaagaatataatgatttttta 632
           ||||||||||||||||||||||||||||||||
Sbjct: 601 caaatgtttctcaagaatataatgatttttta 632


 Score =  131 bits (66), Expect = 1e-30
 Identities = 66/66 (100%)
 Strand = Plus / Plus

                                                                       
Query: 62  tttaaataaaaatgttttcacattgtgaaggatatagaattgaaggtggtgtaactgatg 121
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62  tttaaataaaaatgttttcacattgtgaaggatatagaattgaaggtggtgtaactgatg 121

                 
Query: 122 gagaaa 127
           ||||||
Sbjct: 122 gagaaa 127


>Contig-U11220-1 (Contig-U11220-1Q) /CSM_Contig/Contig-U11220-1Q.Seq.d
          Length = 3459

 Score = 38.2 bits (19), Expect = 0.016
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                               
Query: 334  ttgaagaggtaagagcaaa 352
            |||||||||||||||||||
Sbjct: 1438 ttgaagaggtaagagcaaa 1456


>Contig-U09770-1 (Contig-U09770-1Q) /CSM_Contig/Contig-U09770-1Q.Seq.d
          Length = 1166

 Score = 38.2 bits (19), Expect = 0.016
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 297 gatgatttctatttattag 315
           |||||||||||||||||||
Sbjct: 402 gatgatttctatttattag 420


  Database: CSM
    Posted date:  Jun 7, 2004  8:33 AM
  Number of letters in database: 7,895,291
  Number of sequences in database:  8361
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 11,166
Number of Sequences: 8361
Number of extensions: 11166
Number of successful extensions: 1310
Number of sequences better than 10.0: 149
length of query: 632
length of database: 7,895,291
effective HSP length: 16
effective length of query: 616
effective length of database: 7,761,515
effective search space: 4781093240
effective search space used: 4781093240
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 5. 5
Homology vs DNA 
Query= Contig-U01737-1 (Contig-U01737-1Q) /CSM_Contig/Contig-U01737-1Q.Seq.d
         (632 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU073099) Dictyostelium discoideum slug cDNA, clone SSG137.      121   2e-36  2
(EA102712) Sequence 95 from patent US 7195870.                     44   0.071  2
(AX323619) Sequence 107 from Patent WO0192565.                     44   0.071  2
(AX277932) Sequence 95 from Patent WO0177375.                      44   0.071  2
(GM995967) Sequence 107 from Patent EP2014776.                     44   0.071  2
(CT025843) Medicago truncatula chromosome 5 clone mth2-17e22...    50   0.10   1
(DJ387373) Diagnosis of Diseases Associated with Apoptosis.        38   0.48   2
(AX281281) Sequence 23 from Patent WO0177164.                      38   0.48   2
(DL131170) Bax-responsive genes for drug target identificati...    46   1.6    1
(AX537050) Sequence 651 from Patent WO02064766.                    46   1.6    1
(AR941730) Sequence 651 from patent US 7101990.                    46   1.6    1
(AC160893) Loxodonta africana clone VMRC15-328E9, WORKING DR...    46   1.6    1
(AC116987) Dictyostelium discoideum chromosome 2 map 3527441...    38   2.2    4
(AC201129) Strongylocentrotus purpuratus clone R3-14A21, WOR...    38   3.2    6
(CJ366059) Molgula tectiformis cDNA, cleaving embryo clone:m...    32   3.6    3
(CJ365784) Molgula tectiformis cDNA, cleaving embryo clone:m...    32   4.1    3
(FI259718) CccaBb042-A12JF CccABb Cajanus cajan genomic clon...    36   5.0    2
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...    34   5.3    5
(AC123485) Rattus norvegicus clone CH230-63E14, *** SEQUENCI...    40   5.4    3
(AP009180) Candidatus Carsonella ruddii PV DNA, complete gen...    34   5.7    7
(AY286122) Cyanidioschyzon merolae DBV201 chromosome 1, comp...    44   6.4    1
(AP006483) Cyanidioschyzon merolae DNA, chromosome 1, comple...    44   6.4    1
(AC201790) Zea mays chromosome 6 clone CH201-91O8; ZMMBBc009...    44   6.4    1
(ER831716) POTLK56TF Solanum tuberosum RHPOTKEY BAC ends Sol...    44   6.4    1
(ER566692) 1093015773017 Global-Ocean-Sampling_GS-36-01-01-2...    44   6.4    1
(EJ920757) 1093018631130 Global-Ocean-Sampling_GS-30-02-01-1...    44   6.4    1
(EJ640162) 1093011414351 Global-Ocean-Sampling_GS-29-01-01-1...    44   6.4    1
(EJ629154) 1092963289464 Global-Ocean-Sampling_GS-29-01-01-1...    44   6.4    1
(EC193636) SSE00001816 Streblomastix strix Non-normalized St...    44   6.4    1
(EW977134) EST_sras_evg_784183 srasevg mixed_tissue Sebastes...    44   6.4    1
(CU571197) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    36   8.0    4
(FL498100) Mg_Nor01_64G19 Nor01 Mytilus galloprovincialis cD...    38   9.4    2
(FI248628) CccaBb026-D13JR CccABb Cajanus cajan genomic clon...    36   9.7    2
(AP010899) Solanum lycopersicum DNA, chromosome 8, clone: C0...    38   9.9    4

>(AU073099) Dictyostelium discoideum slug cDNA, clone SSG137.
          Length = 240

 Score =  121 bits (61), Expect(2) = 2e-36
 Identities = 61/61 (100%)
 Strand = Plus / Plus


Query: 75  gttttcacattgtgaaggatatagaattgaaggtggtgtaactgatggagaaacaaattg 134
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75  gttttcacattgtgaaggatatagaattgaaggtggtgtaactgatggagaaacaaattg 134


Query: 135 g 135
           |
Sbjct: 135 g 135

 Score = 67.9 bits (34), Expect(2) = 2e-36
 Identities = 34/34 (100%)
 Strand = Plus / Plus


Query: 207 ttctatggtaaacctcataaaacgtttatgagtt 240
           ||||||||||||||||||||||||||||||||||
Sbjct: 207 ttctatggtaaacctcataaaacgtttatgagtt 240

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 659,353,339
Number of extensions: 39388758
Number of successful extensions: 3191009
Number of sequences better than 10.0: 34
Length of query: 632
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 608
Effective length of database: 99,340,224,878
Effective search space: 60398856725824
Effective search space used: 60398856725824
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.18
Homology vs Protein 
Query= Contig-U01737-1 (Contig-U01737-1Q) /CSM_Contig/Contig-U01737-1Q.Seq.d
         (632 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

HB388609_1(HB388609|pid:none) Sequence 3 from Patent EP2067783.        35   2.3
HB388613_1(HB388613|pid:none) Sequence 7 from Patent EP2067783.        35   2.3
HB388636_1(HB388636|pid:none) Sequence 30 from Patent EP2067783.       35   2.3
HB388646_1(HB388646|pid:none) Sequence 40 from Patent EP2067783.       35   2.3
HB388667_1(HB388667|pid:none) Sequence 61 from Patent EP2067783.       35   2.3
EU839662_1(EU839662|pid:none) Synthetic construct MBP/PPH fusion...    35   2.3
HB388677_1(HB388677|pid:none) Sequence 71 from Patent EP2067783.       35   2.3
HA642537_1(HA642537|pid:none) Sequence 32 from Patent WO20090588...    35   2.3
AX284144_1(AX284144|pid:none) Sequence 1 from Patent WO0178756.        35   2.3
HB388626_1(HB388626|pid:none) Sequence 20 from Patent EP2067783.       35   2.3
HB388616_1(HB388616|pid:none) Sequence 10 from Patent EP2067783.       35   2.3
BC095958_1(BC095958|pid:none) Mus musculus signal-induced prolif...    34   3.1
AB007900_1(AB007900|pid:none) Homo sapiens KIAA0440 mRNA, partia...    34   3.1
AF090989_1(AF090989|pid:none) Homo sapiens high-risk human papil...    34   3.1
AK029889_1(AK029889|pid:none) Mus musculus adult male testis cDN...    34   3.1
BC058681_1(BC058681|pid:none) Mus musculus signal-induced prolif...    34   3.1
AY811540_1(AY811540|pid:none) Schistosoma japonicum SJCHGC02611 ...    34   3.1
FB677256_1(FB677256|pid:none) Sequence 84 from Patent EP1881070.       34   4.0
AX280183_1(AX280183|pid:none) Sequence 29 from Patent WO0177171....    34   4.0
AX478528_1(AX478528|pid:none) Sequence 32 from Patent WO0244209.       34   4.0
FB677260_1(FB677260|pid:none) Sequence 88 from Patent EP1881070.       34   4.0
CU928158_3954(CU928158|pid:none) Escherichia fergusonii ATCC 354...    33   5.2
AY811951_1(AY811951|pid:none) Schistosoma japonicum SJCHGC07988 ...    33   6.8
AF325155_68(AF325155|pid:none) Spodoptera litura nucleopolyhedro...    33   6.8
(Q6CK38) RecName: Full=DNA replication regulator SLD2;                 33   8.9

>HB388609_1(HB388609|pid:none) Sequence 3 from Patent EP2067783.
          Length = 676

 Score = 34.7 bits (78), Expect = 2.3
 Identities = 35/152 (23%), Positives = 65/152 (42%), Gaps = 3/152 (1%)
 Frame = +3

Query: 180 SYGINI*XXFYGKPHKTFMSLSSPNGPILVCIKKVGNSSDDFYLLVCTEKGFEEVRANKD 359
           +YG+ +   F G+P K F+ + S         K++     + YLL  T++G E V  NKD
Sbjct: 242 NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLL--TDEGLEAV--NKD 297

Query: 360 SLQKSKTTRSFLKMRPTSLNVITSVRPELTKVPLCKVTDKSIQEELVKVCEPE---FNKV 530
                         +P     + S   EL K P    T ++ Q+  +    P+   F
Sbjct: 298 --------------KPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYA 343

Query: 531 IKSALLYCKESQRDDNDMLKNISSNVSQEYND 626
           +++A++     ++  ++ LK+  +N S   N+
Sbjct: 344 VRTAVINAASGRQTVDEALKDAQTNSSSNNNN 375

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 669,285,399
Number of extensions: 9957422
Number of successful extensions: 25656
Number of sequences better than 10.0: 25
Number of HSP's gapped: 25647
Number of HSP's successfully gapped: 25
Length of query: 210
Length of database: 1,051,180,864
Length adjustment: 123
Effective length of query: 87
Effective length of database: 653,084,107
Effective search space: 56818317309
Effective search space used: 56818317309
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.89  gvh: 0.38  alm: 0.47  top: 0.37  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U01737-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0