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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10817-1
Contig ID Contig-U10817-1
Contig update 2002.12.18
Contig sequence 
>Contig-U10817-1 (Contig-U10817-1Q) /CSM_Contig/Contig-U10817-1Q.Seq.d
ATTTTTTGTAGCACTATATTAACACCACACATATATATATATATATATAA
ATTAACACAATTTGTACCATATTTAGAATTTAAATAAAAAAGAAGAAAAA
AAATGCAATCTTCTATGTGGAGTTTCTTACAAAAGAAAGAAGGTGGAGAA
ATTGAAGATATAGAAAAGAAAATTAGTAATGCACAAGAATTAAATAAACT
TAAAACTTCCCCTAAAAAGAAAAGAGAAGCAGTGGTTAAAGAAAAAGTTG
AAAAGAAAGAAAAAAAAGAAACAAAACCAAAGAGAAAGAGTTCAAAAAAA
AATAGGGAAGAAGAAGAAGAAGAAGAACAAGAAGAACAAGATGGTGAAGA
AGAACAAGAAGAAGAAGAAGAATATCAACAACAAGATGAAGAAATTGAAG
AAGATATAAATGGTGAAGAAGAAATGGAATTAGATGAAAATGAAAAAGGA
AAGAATAAAAAGAAAAAACAATCATTAAAAACTAAAGAAAATAAAGAATC
AAAATCATCATCTTCTTCAAAGAAAACAATAGAAAATAAAGAAACTAAAA
AACCTGAAAAACAATCTTCAAAACAATCAAATAATTTGAAAAGATTAAAA
AGAAAGAAAATGGATGACGATGAAGAAGACGAAGAAGATGAAAATAAAAC
TGATGATAATGATTTAGATGATATGCTCGATGATGATAGTGATAATGAAA
AAGATTCAATTTCTTC----------AGCAAAGCAAAGCACCACAACCAC
CACCACCACAACTACTACCTCTACCGCCACAACTATTTCTTCAAAATCAA
TTTCAAGTCCATCTAAAAAAGAAGAAAAAGAAGTAATAACTTCAAAAAAA
CAAGTTGAAGCAACAAAAGTAGAAGTTAAAAAAGAAAAAGAAAAAGAAAA
AGAAAAAGAAAAAGAATCAAAGGAAAAAGAAAAGAAAGAAAAAGAAAAAA
AAGAAAAAGAAGAAAAAGATAAAAAAGAAAAGGAATTAAAAGAAAAGGAA
TTAAAAGAAAAGGAATTAAAAGATAAAAAAGAAAAGGAGCTAAAAGAAAA
AGAAAAAGAACTCAAAGCATAAAGAGAAAAAAAGAAAAAGAGTTAAAAGA
AAAAGAGAAAAAAGAAAAGGAAGAAAAGGAAAAGGAGAAAAAAAGAGAAA
AAAGAAAAAGAATTGAAAGAAAAAGAAGAAAAAGAGAAAAAAGAAAAGGA
ATTAAAAGAAAAGGAATTAAAAGAAAAGGAATTAAAAGAAAAGGAATTAA
AAGAAAAGGAATTAACTTCACCAAAAAAAGAAACTATTGATATTTCAGAT
TTATTTAAAAGAGCAAATGCTGAAGCTAAATCGTCAGTACCAACCTCAAC
ATCTAAAAACAGTAAAACTAATAAAAAACAAAAAGTTGATCATAAACCAA
CAGCAACAACAAAAAAAACCATCACCTGTACTTGAAGCAAAGCAAAGCAC
CACAACCACCACCACCACAACTACTACCTCTACCGCCACAACTATTTCTT
CAAAATCAATTTCAAGTCCATCTAAAAAAGAAGAAAAAGAAGTAATAACT
TCAAAAAAACAAGTTGAAGCAACAAAAGTAGAAGTTAAAAAAGAAAAAGA
AAAAGAAAAAGAAAAAGAAAAAGAAGATGATGAGGAAGAAGAGGAAGAAG
AAGAAGATGATGATGAAAAATTAGAATATATTGATGAGGAAGAATATGAA
GAAGAAGAAGAAGAAGATGAAGAGGGAATTAGTGAAAATGAAGAAGAAGA
AGAAAAGAAAAGTACACAAATTAAAAGTAAATTTATTAAAAAAGTACCAA
TTTCAAAAAAGAAAGGTAATGCAAAAACTATTCAAGCTGATTTAAAGGTT
ATTGGAAAATATAGACCAATTGAGGATGCACAATGGAAAAAAGGTGAAGC
TGTACCATATATGGTATTAGCAAAAACCTTTGAAATGATGGAATCAACAT
CATCAAGATTAATTATTAT
Gap gap included
Contig length 1959
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 13
Number of EST 17
Link to clone list U10817
List of clone(s) 

est1=CHR561F,1,324
est2=AHM362F,2,309
est3=AFD754F,8,370
est4=AFL413F,8,606
est5=CHM678F,9,337
est6=SHB161F,35,627
est7=CHM131F,61,339
est8=SLG880F,284,717
est9=AFD754Z,718,1083
est10=AFL413Z,739,1532
est11=AHD760F,797,1419
est12=CHM131Z,818,1133
est13=SHB326F,1061,1583
est14=SLG880Z,1173,1583
est15=AHA838F,1174,1745
est16=SLC554Z,1293,1819
est17=AHM654F,1360,1959
Translated Amino Acid sequence 
ifcstiltphiyiyiykltqfvpylefk*KRRKKMQSSMWSFLQKKEGGEIEDIEKKISN
AQELNKLKTSPKKKREAVVKEKVEKKEKKETKPKRKSSKKNREEEEEEEQEEQDGEEEQE
EEEEYQQQDEEIEEDINGEEEMELDENEKGKNKKKKQSLKTKENKESKSSSSSKKTIENK
ETKKPEKQSSKQSNNLKRLKRKKMDDDEEDEEDENKTDDNDLDDMLDDDSDNEKDSIS--
-

---AKQSTTTTTTTTTTSTATTISSKSISSPSKKEEKEVITSKKQVEATKVEVKKEKEKE
KEKEKESKEKEKKEKEKKEKEEKDKKEKELKEKELKEKELKDKKEKELKEKEKELKA*re
kkkks*kkkrkkkrkkrkrrkkekkekelkekeekekkekelkekelkekelkekelkek
eltspkketidisdlfkranaeakssvptstsknsktnkkqkvdhkptattkktitct*s
kakhhnhhhhnyylyrhnyffkinfksi*krrkrsnnfkkts*snksrs*krkrkrkrkr
krr**grrgrrrr***kiriy**gri*rrrrrr*rgn**k*rrrrkekytn*k*iy*kst
nfkker*cknyss*fkgywki*tn*gctmekr*sctiygisknl*ndginiikinyy
Translated Amino Acid sequence (All Frames) 
Frame A:
ifcstiltphiyiyiykltqfvpylefk*KRRKKMQSSMWSFLQKKEGGEIEDIEKKISN
AQELNKLKTSPKKKREAVVKEKVEKKEKKETKPKRKSSKKNREEEEEEEQEEQDGEEEQE
EEEEYQQQDEEIEEDINGEEEMELDENEKGKNKKKKQSLKTKENKESKSSSSSKKTIENK
ETKKPEKQSSKQSNNLKRLKRKKMDDDEEDEEDENKTDDNDLDDMLDDDSDNEKDSIS--
-

---skakhhnhhhhnyylyrhnyffkinfksi*krrkrsnnfkkts*snksrs*krkrkr
krkrkrikgkrkerkrkkrkrrkr*krkgikrkgikrkgikr*krkgakrkrkrtqsikr
kkekelkekekkekeekekekkrekrkrierkrrkrekrkgikrkgikrkgikrkgikrk
ginftkkrny*yfrfi*kskc*s*ivstnlni*kq*n**ktks*s*tnsnnkknhhlylk
qskapqppppqllplppqlflqnqfqvhlkkkkkk**lqknklkqqk*klkkkkkkkkkk
kkkmmrkkrkkkkmmmkn*nilmrknmkkkkkkmkrelvkmkkkkkrkvhklkvnllkky
qfqkrkvmqklfkli*rllenidqlrmhngkkvklyhiwy*qkplk*wnqhhqd*ll

Frame B:
ffvaly*hhtyiyiyin*hnlyhi*nlnkkeekkcnllcgvsykrkkveklki*krklvm
hkn*inlklplkrkekqwlkkklkrkkkkkqnqrervqkkigkkkkkknkknkmvkknkk
kkkninnkmkklkki*mvkkkwn*mkmkkerikrknnh*klkkiknqnhhllqrkq*kik
klknlknnlqnnqii*kd*kerkwmtmkktkkmkiklmimi*micsmmivimkkiqfl--
-

---AKQSTTTTTTTTTTSTATTISSKSISSPSKKEEKEVITSKKQVEATKVEVKKEKEKE
KEKEKESKEKEKKEKEKKEKEEKDKKEKELKEKELKEKELKDKKEKELKEKEKELKA*re
kkkks*kkkrkkkrkkrkrrkkekkekelkekeekekkekelkekelkekelkekelkek
eltspkketidisdlfkranaeakssvptstsknsktnkkqkvdhkptattkktitct*s
kakhhnhhhhnyylyrhnyffkinfksi*krrkrsnnfkkts*snksrs*krkrkrkrkr
krr**grrgrrrr***kiriy**gri*rrrrrr*rgn**k*rrrrkekytn*k*iy*kst
nfkker*cknyss*fkgywki*tn*gctmekr*sctiygisknl*ndginiikinyy

Frame C:
fl*hyintthiyiyi*intictifri*ikkkkknaifyvefltkerrwrn*ryrken**c
trik*t*nfp*kekrssg*rks*kerkkrnktkekefkkk*grrrrrrtrrtrw*rrtrr
rrristtr*rn*rrykw*rrngir*k*krke*kektiikn*rk*rikiiiffkennrk*r
n*kt*ktifktik*fekikkkeng*r*rrrrr*k*n****fr*yar******krfnff--
-

---qskapqppppqllplppqlflqnqfqvhlkkkkkk**lqknklkqqk*klkkkkkkk
kkkkknqrkkkrkkkkkkkkkkkikkkrn*kkrn*kkrn*kikkkrs*kkkkknskhkek
krkrvkrkrekrkgrkgkgekkrkkkkn*kkkkkkrkkkrn*kkrn*kkrn*kkrn*kkr
n*lhqkkkllifqiylkeqmlklnrqyqpqhlktvkliknkkliinqqqqqkkpspvlea
kqsttttttttttstattissksisspskkeekevitskkqveatkvevkkekekekeke
keddeeeeeeeedddekleyideeeyeeeeeedeegiseneeeeekkstqikskfikkvp
iskkkgnaktiqadlkvigkyrpiedaqwkkgeavpymvlaktfemmestssrlii
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10817-1 (Contig-U10817-1Q)
/CSM_Contig/Contig-U10817-1Q.Seq.d
         (1969 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10817-1 (Contig-U10817-1Q) /CSM_Contig/Conti...   283  4e-76
Contig-U14742-1 (Contig-U14742-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U11146-1 (Contig-U11146-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U08927-1 (Contig-U08927-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U14746-1 (Contig-U14746-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U12197-1 (Contig-U12197-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U11302-1 (Contig-U11302-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U14907-1 (Contig-U14907-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U14159-1 (Contig-U14159-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U12505-1 (Contig-U12505-1Q) /CSM_Contig/Conti...    40  0.009

>Contig-U10817-1 (Contig-U10817-1Q) /CSM_Contig/Contig-U10817-1Q.Seq.d
          Length = 1969

 Score =  283 bits (143), Expect = 4e-76
 Identities = 159/167 (95%)
 Strand = Plus / Plus

                                                                        
Query: 1385 gttgatcataaaccaacagcaacaacnnnnnnnnccatcacctgtacttgaagcaaagca 1444
            ||||||||||||||||||||||||||        ||||||||||||||||||||||||||
Sbjct: 1385 gttgatcataaaccaacagcaacaacaaaaaaaaccatcacctgtacttgaagcaaagca 1444

                                                                        
Query: 1445 aagcaccacaaccaccaccaccacaactactacctctaccgccacaactatttcttcaaa 1504
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1445 aagcaccacaaccaccaccaccacaactactacctctaccgccacaactatttcttcaaa 1504

                                                           
Query: 1505 atcaatttcaagtccatctaaaaaagaagaaaaagaagtaataactt 1551
            |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1505 atcaatttcaagtccatctaaaaaagaagaaaaagaagtaataactt 1551


 Score =  281 bits (142), Expect = 1e-75
 Identities = 142/142 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1828 ctattcaagctgatttaaaggttattggaaaatatagaccaattgaggatgcacaatgga 1887
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1828 ctattcaagctgatttaaaggttattggaaaatatagaccaattgaggatgcacaatgga 1887

                                                                        
Query: 1888 aaaaaggtgaagctgtaccatatatggtattagcaaaaacctttgaaatgatggaatcaa 1947
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1888 aaaaaggtgaagctgtaccatatatggtattagcaaaaacctttgaaatgatggaatcaa 1947

                                  
Query: 1948 catcatcaagattaattattat 1969
            ||||||||||||||||||||||
Sbjct: 1948 catcatcaagattaattattat 1969


 Score =  274 bits (138), Expect = 3e-73
 Identities = 168/168 (100%)
 Strand = Plus / Plus

                                                                       
Query: 645 taaaactgatgataatgatttagatgatatgctcgatgatgatagtgataatgaaaaaga 704
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 645 taaaactgatgataatgatttagatgatatgctcgatgatgatagtgataatgaaaaaga 704

                                                                       
Query: 705 ttcaatttcttcnnnnnnnnnnagcaaagcaaagcaccacaaccaccaccaccacaacta 764
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 705 ttcaatttcttcnnnnnnnnnnagcaaagcaaagcaccacaaccaccaccaccacaacta 764

                                                           
Query: 765 ctacctctaccgccacaactatttcttcaaaatcaatttcaagtccat 812
           ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 765 ctacctctaccgccacaactatttcttcaaaatcaatttcaagtccat 812


 Score =  230 bits (116), Expect = 5e-60
 Identities = 116/116 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1436 agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 1495
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 727  agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 786

                                                                    
Query: 1496 ttcttcaaaatcaatttcaagtccatctaaaaaagaagaaaaagaagtaataactt 1551
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 787  ttcttcaaaatcaatttcaagtccatctaaaaaagaagaaaaagaagtaataactt 842


 Score =  216 bits (109), Expect = 7e-56
 Identities = 109/109 (100%)
 Strand = Plus / Plus

                                                                       
Query: 104 tgcaatcttctatgtggagtttcttacaaaagaaagaaggtggagaaattgaagatatag 163
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 104 tgcaatcttctatgtggagtttcttacaaaagaaagaaggtggagaaattgaagatatag 163

                                                            
Query: 164 aaaagaaaattagtaatgcacaagaattaaataaacttaaaacttcccc 212
           |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 164 aaaagaaaattagtaatgcacaagaattaaataaacttaaaacttcccc 212


 Score =  170 bits (86), Expect = 4e-42
 Identities = 86/86 (100%)
 Strand = Plus / Plus

                                                                        
Query: 727  agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 786
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1436 agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 1495

                                      
Query: 787  ttcttcaaaatcaatttcaagtccat 812
            ||||||||||||||||||||||||||
Sbjct: 1496 ttcttcaaaatcaatttcaagtccat 1521


 Score =  147 bits (74), Expect = 5e-35
 Identities = 74/74 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1280 gaaactattgatatttcagatttatttaaaagagcaaatgctgaagctaaatcgtcagta 1339
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1280 gaaactattgatatttcagatttatttaaaagagcaaatgctgaagctaaatcgtcagta 1339

                          
Query: 1340 ccaacctcaacatc 1353
            ||||||||||||||
Sbjct: 1340 ccaacctcaacatc 1353


 Score = 71.9 bits (36), Expect = 3e-12
 Identities = 36/36 (100%)
 Strand = Plus / Plus

                                              
Query: 50 aattaacacaatttgtaccatatttagaatttaaat 85
          ||||||||||||||||||||||||||||||||||||
Sbjct: 50 aattaacacaatttgtaccatatttagaatttaaat 85


 Score = 60.0 bits (30), Expect = 1e-08
 Identities = 30/30 (100%)
 Strand = Plus / Plus

                                        
Query: 1  attttttgtagcactatattaacaccacac 30
          ||||||||||||||||||||||||||||||
Sbjct: 1  attttttgtagcactatattaacaccacac 30


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                            
Query: 1523 taaaaaagaagaaaaa 1538
            ||||||||||||||||
Sbjct: 85   taaaaaagaagaaaaa 100


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                            
Query: 1525 aaaaagaagaaaaaga 1540
            ||||||||||||||||
Sbjct: 1170 aaaaagaagaaaaaga 1185


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                            
Query: 1525 aaaaagaagaaaaaga 1540
            ||||||||||||||||
Sbjct: 954  aaaaagaagaaaaaga 969


>Contig-U14742-1 (Contig-U14742-1Q) /CSM_Contig/Contig-U14742-1Q.Seq.d
          Length = 1266

 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 28/30 (93%)
 Strand = Plus / Plus

                                          
Query: 739  caccacaaccaccaccaccacaactactac 768
            |||||| ||||||||||| |||||||||||
Sbjct: 1072 caccaccaccaccaccacaacaactactac 1101


 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 28/30 (93%)
 Strand = Plus / Plus

                                          
Query: 1448 caccacaaccaccaccaccacaactactac 1477
            |||||| ||||||||||| |||||||||||
Sbjct: 1072 caccaccaccaccaccacaacaactactac 1101


>Contig-U11146-1 (Contig-U11146-1Q) /CSM_Contig/Contig-U11146-1Q.Seq.d
          Length = 2547

 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 31/34 (91%)
 Strand = Plus / Minus

                                              
Query: 1456 ccaccaccaccacaactactacctctaccgccac 1489
            ||||||||||||| ||||||||| ||||| ||||
Sbjct: 197  ccaccaccaccactactactaccactaccaccac 164


 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 31/34 (91%)
 Strand = Plus / Minus

                                             
Query: 747 ccaccaccaccacaactactacctctaccgccac 780
           ||||||||||||| ||||||||| ||||| ||||
Sbjct: 197 ccaccaccaccactactactaccactaccaccac 164


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 28/32 (87%)
 Strand = Plus / Minus

                                            
Query: 1458 accaccaccacaactactacctctaccgccac 1489
            ||||||||||| ||||| ||| ||||| ||||
Sbjct: 1913 accaccaccaccactaccaccactaccaccac 1882


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                                
Query: 739  caccacaaccaccaccacca 758
            |||||| |||||||||||||
Sbjct: 1896 caccactaccaccaccacca 1877


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 28/32 (87%)
 Strand = Plus / Minus

                                            
Query: 749  accaccaccacaactactacctctaccgccac 780
            ||||||||||| ||||| ||| ||||| ||||
Sbjct: 1913 accaccaccaccactaccaccactaccaccac 1882


 Score = 32.2 bits (16), Expect = 2.2
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                                
Query: 1448 caccacaaccaccaccacca 1467
            |||||| |||||||||||||
Sbjct: 1896 caccactaccaccaccacca 1877


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 17,106
Number of Sequences: 6905
Number of extensions: 17106
Number of successful extensions: 3864
Number of sequences better than 10.0: 417
length of query: 1969
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1953
effective length of database: 5,564,391
effective search space: 10867255623
effective search space used: 10867255623
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.22
Homology vs DNA 
Query= Contig-U10817-1 (Contig-U10817-1Q) /CSM_Contig/Contig-U10817-1Q.Seq.d
         (1969 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   281   0.0    8
(BJ337207) Dictyostelium discoideum cDNA clone:dda56k14, 5' ...   281   e-124  2
(AU039977) Dictyostelium discoideum slug cDNA, clone SLG880.      206   4e-82  2
(BJ392071) Dictyostelium discoideum cDNA clone:dds26d07, 5' ...   206   6e-82  2
(BJ330687) Dictyostelium discoideum cDNA clone:dda32l10, 5' ...   206   6e-82  2
(BJ409106) Dictyostelium discoideum cDNA clone:dds39o05, 3' ...   200   5e-80  2
(BJ345253) Dictyostelium discoideum cDNA clone:dda23j04, 3' ...   147   3e-75  4
(BJ347381) Dictyostelium discoideum cDNA clone:dda26n22, 3' ...   135   5e-57  2
(AU034306) Dictyostelium discoideum slug cDNA, clone SLC554.      206   2e-48  1
(BJ341900) Dictyostelium discoideum cDNA clone:dda8l13, 3' e...   200   1e-46  1
(BJ371342) Dictyostelium discoideum cDNA clone:ddc57i15, 5' ...    98   1e-43  3
(BJ328147) Dictyostelium discoideum cDNA clone:dda23j04, 5' ...   109   5e-43  3
(BJ324906) Dictyostelium discoideum cDNA clone:dda8l13, 5' e...   101   1e-40  3
(BJ368731) Dictyostelium discoideum cDNA clone:ddc47k20, 5' ...    98   4e-37  3
(BJ392157) Dictyostelium discoideum cDNA clone:dds26i15, 5' ...   115   2e-36  2
(BJ336925) Dictyostelium discoideum cDNA clone:dda55l15, 5' ...   103   6e-33  2
(BJ332286) Dictyostelium discoideum cDNA clone:dda38h15, 5' ...   139   1e-28  2
(BJ329979) Dictyostelium discoideum cDNA clone:dda26n22, 5' ...   115   2e-23  2
(BJ368367) Dictyostelium discoideum cDNA clone:ddc46m07, 5' ...   107   9e-22  2
(EU094832) Humulus lupulus clone ACC4-C22-9 microsatellite s...    62   2e-09  2
(EA497047) Sequence 16 from patent US 7357928.                     48   1e-08  2
(EA374751) Sequence 13 from patent US 7314926.                     48   1e-08  2
(DL075987) A METHOD FOR THE TREATMENT OF MALIGNANT DISEASES ...    48   1e-08  2
(DD251101) A METHOD FOR THE DETECTION OF APOPTOSIS.                48   1e-08  2
(DD240944) A NEW METHOD FOR THE DIAGNOSIS AND PROGNOSIS OF M...    48   1e-08  2
(CS097979) Sequence 17 from Patent WO2005037323.                   48   1e-08  2
(CS077898) Sequence 16 from Patent WO2005035579.                   48   1e-08  2
(BD252109) Antiproliferative activity of G-rich oligonucleot...    48   1e-08  2
(AC114113) Rattus norvegicus clone CH230-186O3, WORKING DRAF...    54   2e-08  2
(AZ800828) 2M0059K04F Mouse 10kb plasmid UUGC1M library Mus ...    48   2e-07  2
(AJ403997) Hyla arborea microsatellite DNA, clone WHA5-29.         48   2e-07  2
(AC112456) Rattus norvegicus clone CH230-110N23, *** SEQUENC...    54   4e-07  3
(AY298907) Trypanosoma cruzi cosmid C5 mucin-like protein ge...    52   8e-07  2
(AC117045) Rattus norvegicus clone CH230-384C18, WORKING DRA...    54   9e-07  2
(DH495319) Monosiga ovata DNA, fosmid clone: MOF-059D18, gen...    50   1e-06  2
(DH479879) Monosiga ovata DNA, fosmid clone: MOF-032E11, gen...    48   3e-06  2
(DH503802) Monosiga ovata DNA, fosmid clone: MOF-073N16, gen...    48   3e-06  2
(DH477855) Monosiga ovata DNA, fosmid clone: MOF-028L23, gen...    48   3e-06  2
(AC126091) Rattus norvegicus clone CH230-159D12, WORKING DRA...    54   3e-06  2
(DH478663) Monosiga ovata DNA, fosmid clone: MOF-030D09, gen...    52   4e-06  2
(DH497552) Monosiga ovata DNA, fosmid clone: MOF-063C09, gen...    46   4e-06  2
(AC122162) Medicago truncatula clone mth2-16g15, complete se...    54   7e-06  2
(AY145463) Bemisia tabaci microsatellite Bem31 sequence.           48   8e-06  2
(CD342650) EtESTee54g04.y1 Eimeria tenella M5-6 Excised cDNA...    50   9e-06  2
(DE236563) Trifolium pratense DNA, clone:RCG32026.                 46   9e-06  2
(AC115904) Mus musculus chromosome 16, clone RP24-472P22, co...    54   1e-05  3
(AC128087) Rattus norvegicus clone CH230-347E19, *** SEQUENC...    52   1e-05  2
(BQ518799) EST626202 Generation of a set of potato cDNA clon...    48   1e-05  2
(DC490513) Monosiga ovata cDNA, clone: MOC-058L17, 5'end.          50   1e-05  2
(DC515352) Monosiga ovata cDNA, clone: MOC-094K01, 5'end.          50   1e-05  2
(AK278037) Gryllus bimaculatus mRNA, GBcontig24898.                42   2e-05  3
(AC103260) Rattus norvegicus clone CH230-168O17, *** SEQUENC...    52   2e-05  2
(AY383694) Rattus norvegicus LRRGT00039 mRNA, complete cds.        54   2e-05  2
(BM306648) EtESTee34a01.y1 Eimeria tenella M5-6 cDNA Neg Sel...    48   3e-05  2
(AK275793) Gryllus bimaculatus mRNA, GBcontig22654.                44   3e-05  2
(BM305409) EtESTee30g03.y1 Eimeria tenella M5-6 cDNA Neg Sel...    48   3e-05  2
(DH489849) Monosiga ovata DNA, fosmid clone: MOF-049G18, gen...    44   3e-05  2
(AI757534) EtESTea17h06.y1 Eimeria M5-6 Merozoite stage Eime...    48   3e-05  2
(DE238170) Trifolium pratense DNA, clone:RCG34661.                 44   3e-05  2
(CD346804) EtESTee97e04.y1 Eimeria tenella M5-6 Excised cDNA...    48   4e-05  2
(CD659074) EtESTef46c11.y1 Eimeria tenella M5-6 Excised cDNA...    48   4e-05  2
(CD342392) EtESTee51h10.y1 Eimeria tenella M5-6 Excised cDNA...    48   4e-05  2
(DH496923) Monosiga ovata DNA, fosmid clone: MOF-061P18, gen...    44   4e-05  2
(AC064811) Homo sapiens chromosome 4 clone RP11-638N24 map 4...    52   4e-05  2
(DH503003) Monosiga ovata DNA, fosmid clone: MOF-072H20, gen...    44   4e-05  2
(AC121346) Rattus norvegicus chromosome 1 clone RP32-276L6 m...    48   5e-05  2
(CK257824) EST741461 potato callus cDNA library, normalized ...    48   5e-05  2
(DH477779) Monosiga ovata DNA, fosmid clone: MOF-028J10, gen...    44   5e-05  2
(AC133097) Mus musculus BAC clone RP23-287O2 from 1, complet...    48   5e-05  3
(AC127130) Rattus norvegicus clone CH230-118H23, *** SEQUENC...    54   5e-05  2
(DE941155) Macropus eugenii DNA, BAC clone: MEB1-135K03_R, 3...    44   5e-05  2
(AC097248) Rattus norvegicus clone CH230-177C11, *** SEQUENC...    48   5e-05  2
(DE879302) Macropus eugenii DNA, BAC clone: MEB1-051P03_F, 5...    48   6e-05  2
(AC095114) Rattus norvegicus clone CH230-7L7, WORKING DRAFT ...    48   6e-05  2
(AC107254) Rattus norvegicus clone CH230-74B18, *** SEQUENCI...    50   6e-05  2
(AC113647) Rattus norvegicus clone CH230-325A20, *** SEQUENC...    48   6e-05  2
(DE221803) Trifolium pratense DNA, clone:RCG13118.                 44   1e-04  2
(AL445933) Human DNA sequence from clone RP11-486B10 on chro...    52   1e-04  2
(EC714199) MCE00001937 Malawimonas californiana Home made (N...    46   1e-04  2
(DV604649) EST1207645 Glossina morsitans morsitans Fat body ...    58   2e-04  2
(DE242541) Trifolium pratense DNA, clone:RCG48685.                 46   2e-04  2
(DH502270) Monosiga ovata DNA, fosmid clone: MOF-071E23, gen...    44   2e-04  2
(DH464435) Monosiga ovata DNA, fosmid clone: MOF-005B13, gen...    46   2e-04  2
(DH505345) Monosiga ovata DNA, fosmid clone: MOF-076G19, gen...    44   2e-04  2
(CO543628) LyEST11252 Sea lamprey LyEST Petromyzon marinus c...    46   2e-04  2
(DH503426) Monosiga ovata DNA, fosmid clone: MOF-073D10, gen...    44   2e-04  2
(EU267685) Portunus trituberculatus clone ptssr14 microsatel...    42   3e-04  2
(DQ514584) Nasutitermes corniger microsatellite Ncor1 sequence.    42   3e-04  2
(AF192260) Periplaneta fuliginosa densovirus, complete genome.     44   3e-04  2
(AB028936) Periplaneta fuliginosa densovirus ORF-beta, ORF-a...    44   3e-04  2
(AL078584) Human DNA sequence from clone RP1-245M18 on chrom...    46   4e-04  2
(AI755861) EtESTea08c10.y1 Eimeria M5-6 Merozoite stage Eime...    44   4e-04  2
(EI706315) IGBR05F01TR BAC library from the recurrent gliobl...    46   5e-04  2
(DW249894) Cm_mx1_28a04_SP6 Green Shore Crab Multiple Tissue...    44   5e-04  2
(DQ191761) Monosiga ovata Hoglet (hoglet) mRNA, complete cds.      44   5e-04  2
(FE749859) CAYC657.g1 CAYC Petrolisthes cinctipes heart, ner...    46   5e-04  2
(CK253857) EST737494 potato callus cDNA library, normalized ...    44   5e-04  2
(AC177073) Strongylocentrotus purpuratus clone R3-4004K17, W...    46   5e-04  2
(AC146997) Arbacia punctulata clone BAP-144G2, WORKING DRAFT...    46   6e-04  2
(CT476835) Human DNA sequence from clone CH507-286C5 on chro...    46   6e-04  2

>(AC116957) Dictyostelium discoideum chromosome 2 map 1685067-2090751
              strain AX4, complete sequence.
          Length = 405682

 Score =  281 bits (142), Expect(8) = 0.0
 Identities = 142/142 (100%)
 Strand = Plus / Plus


Query: 1828   ctattcaagctgatttaaaggttattggaaaatatagaccaattgaggatgcacaatgga 1887
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 255403 ctattcaagctgatttaaaggttattggaaaatatagaccaattgaggatgcacaatgga 255462


Query: 1888   aaaaaggtgaagctgtaccatatatggtattagcaaaaacctttgaaatgatggaatcaa 1947
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 255463 aaaaaggtgaagctgtaccatatatggtattagcaaaaacctttgaaatgatggaatcaa 255522


Query: 1948   catcatcaagattaattattat 1969
              ||||||||||||||||||||||
Sbjct: 255523 catcatcaagattaattattat 255544

 Score =  206 bits (104), Expect(8) = 0.0
 Identities = 104/104 (100%)
 Strand = Plus / Plus


Query: 1420   catcacctgtacttgaagcaaagcaaagcaccacaaccaccaccaccacaactactacct 1479
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 254995 catcacctgtacttgaagcaaagcaaagcaccacaaccaccaccaccacaactactacct 255054


Query: 1480   ctaccgccacaactatttcttcaaaatcaatttcaagtccatct 1523
              ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 255055 ctaccgccacaactatttcttcaaaatcaatttcaagtccatct 255098

 Score =  139 bits (70), Expect(8) = 0.0
 Identities = 70/70 (100%)
 Strand = Plus / Plus


Query: 1284   ctattgatatttcagatttatttaaaagagcaaatgctgaagctaaatcgtcagtaccaa 1343
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 254860 ctattgatatttcagatttatttaaaagagcaaatgctgaagctaaatcgtcagtaccaa 254919


Query: 1344   cctcaacatc 1353
              ||||||||||
Sbjct: 254920 cctcaacatc 254929

 Score = 85.7 bits (43), Expect(8) = 0.0
 Identities = 43/43 (100%)
 Strand = Plus / Plus


Query: 119    ggagtttcttacaaaagaaagaaggtggagaaattgaagatat 161
              |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 253810 ggagtttcttacaaaagaaagaaggtggagaaattgaagatat 253852

 Score = 71.9 bits (36), Expect(8) = 0.0
 Identities = 36/36 (100%)
 Strand = Plus / Plus


Query: 50     aattaacacaatttgtaccatatttagaatttaaat 85
              ||||||||||||||||||||||||||||||||||||
Sbjct: 253616 aattaacacaatttgtaccatatttagaatttaaat 253651

 Score = 60.0 bits (30), Expect(8) = 0.0
 Identities = 30/30 (100%)
 Strand = Plus / Plus


Query: 1      attttttgtagcactatattaacaccacac 30
              ||||||||||||||||||||||||||||||
Sbjct: 253569 attttttgtagcactatattaacaccacac 253598

 Score = 34.2 bits (17), Expect(8) = 0.0
 Identities = 17/17 (100%)
 Strand = Plus / Plus


Query: 104    tgcaatcttctatgtgg 120
              |||||||||||||||||
Sbjct: 253670 tgcaatcttctatgtgg 253686

 Score = 28.2 bits (14), Expect(8) = 0.0
 Identities = 14/14 (100%)
 Strand = Plus / Plus


Query: 703    gattcaatttcttc 716
              ||||||||||||||
Sbjct: 254394 gattcaatttcttc 254407

 Score =  174 bits (88), Expect = 8e-39
 Identities = 88/88 (100%)
 Strand = Plus / Plus


Query: 727    agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 786
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 255011 agcaaagcaaagcaccacaaccaccaccaccacaactactacctctaccgccacaactat 255070


Query: 787    ttcttcaaaatcaatttcaagtccatct 814
              ||||||||||||||||||||||||||||
Sbjct: 255071 ttcttcaaaatcaatttcaagtccatct 255098

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 674,258,730
Number of extensions: 48876991
Number of successful extensions: 5851362
Number of sequences better than 10.0: 3154
Length of query: 1969
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1945
Effective length of database: 97,308,875,965
Effective search space: 189265763751925
Effective search space used: 189265763751925
X1: 11 (21.8 bits)
S2: 23 (46.1 bits)
protein update 2009. 6.28
Homology vs Protein 
Query= Contig-U10817-1 (Contig-U10817-1Q) /CSM_Contig/Contig-U10817-1Q.Seq.d
         (1969 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FN317154_1(FN317154|pid:none) Schistosoma japonicum isolate Anhu...    54   3e-05
AY809771_1(AY809771|pid:none) Schistosoma japonicum SJCHGC08518 ...    54   3e-05
FN357328_36(FN357328|pid:none) Schistosoma mansoni genome sequen...    50   4e-04
FN357328_37(FN357328|pid:none) Schistosoma mansoni genome sequen...    50   4e-04
AK303644_1(AK303644|pid:none) Homo sapiens cDNA FLJ52385 complet...    48   0.001
AB208791_1(AB208791|pid:none) Homo sapiens mRNA for DNA ligase I...    48   0.001
(P18858) RecName: Full=DNA ligase 1;          EC=6.5.1.1; AltNam...    48   0.001
BC110622_1(BC110622|pid:none) Homo sapiens ligase I, DNA, ATP-de...    48   0.001
(P37913) RecName: Full=DNA ligase 1;          EC=6.5.1.1; AltNam...    47   0.003
AK161793_1(AK161793|pid:none) Mus musculus 13 days embryo male t...    47   0.003
(Q9JHY8) RecName: Full=DNA ligase 1;          EC=6.5.1.1; AltNam...    47   0.003
BX005060_7(BX005060|pid:none) Zebrafish DNA sequence from clone ...    46   0.004
(P51892) RecName: Full=DNA ligase 1;          EC=6.5.1.1; AltNam...    46   0.004
AY051939_1(AY051939|pid:none) Drosophila melanogaster LD41868 fu...    41   0.14
(Q9W1H4) RecName: Full=DNA ligase 1;          EC=6.5.1.1; AltNam...    41   0.14
CP000590_67(CP000590|pid:none) Ostreococcus lucimarinus CCE9901 ...    39   0.69
AP007171_676(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...    39   0.90
AB072813_1(AB072813|pid:none) Coprinopsis cinerea mRNA for DNA l...    38   1.5
CU633900_875(CU633900|pid:none) Podospora anserina genomic DNA c...    37   2.6
CP001323_748(CP001323|pid:none) Micromonas sp. RCC299 chromosome...    37   2.6

>FN317154_1(FN317154|pid:none) Schistosoma japonicum isolate Anhui
            full length mRNA clone SJFCE0674|FSE_044_94_F12, complete
            sequence.
          Length = 840

 Score = 53.5 bits (127), Expect = 3e-05
 Identities = 23/36 (63%), Positives = 29/36 (80%)
 Frame = +3

Query: 1860 YRPIEDAQWKKGEAVPYMVLAKTFEMMESTSSRLII 1967
            Y PI D  WK+GE+VPY+ LAKTFE +E+TSSR+ I
Sbjct: 160  YHPIHDCNWKEGESVPYLSLAKTFECIETTSSRIKI 195

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 848,305,063
Number of extensions: 10329160
Number of successful extensions: 27679
Number of sequences better than 10.0: 20
Number of HSP's gapped: 27481
Number of HSP's successfully gapped: 20
Length of query: 656
Length of database: 1,040,966,779
Length adjustment: 135
Effective length of query: 521
Effective length of database: 608,388,304
Effective search space: 316970306384
Effective search space used: 316970306384
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.86  gvh: 0.23  alm: 0.63  top: 0.07  tms: 0.00  mit: 0.36  mip: 0.02
 nuc: 0.87  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for Contig-U10817-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 4
AF (FL, S) 2
SL (DIR, L) 2
SS (DIR, S) 0
SH (FL, L) 2
SF (FL, S) 0
CH (FL, L) 3
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0