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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10424-1
Contig ID Contig-U10424-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10424-1 (Contig-U10424-1Q) /CSM_Contig/Contig-U10424-1Q.Seq.d
ACTGTTGGCCTACTGGAGGTTTGGTTTTATCAACTATTCAAAACAAATAT
AATAAAATGCAAGCTGCAGTAGCATCTAAAACAAATATTTCATTAAAAGG
TTCAACAGAGATTGTAACTGAATTTTTTTCATATAGTATAAATACAATTC
TATTTCAAAGAGGATTATATCCACCAGAATCATTTACAAGAGTTGCAAAA
TATGGATTACCAATATTGGTAACTAATGATCAATCATTAAAAGATTATTT
AGATAATGTATTAAAACAATTATCAGAATGGTTATTAAGTGGTGATGTTC
AAAAATTGGTTTTAGTAATCACTGATATCGTTACAAAAGAGGTTTTAGAA
AGATGGGTATTCGATGTTACAACTGATATTCCAAAAGAAGGAGAAGCACC
AAGACAAAAACCAGAAAAAGAGATAATGAATGAAATTCAAGCAATTATTA
GACAAATTACAGCAAGTGTTACATTTTTACCACTTTTACCAAATGCATGT
ACATTTGATTTATTAGTATATACAAGTAAAGATTTAGCAGTACCTCAAAA
ATGGGAAGAATCTGATCCAAAATTCATAACAAATTCACAACAAGTTAAAC
TTAGATCTTTTACAACAACAATTCATAAAGTTGAAAGTATGGTTGCCTAT
AAAATTTCAAAGATTAAAATAATAAAATAAATAAATAT
Gap no gap
Contig length 688
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 3310280
End point 3309609
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U10424
List of clone(s) 

est1=VFI627E,1,689
Translated Amino Acid sequence 
CWPTGGLVLSTIQNKYNKMQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPES
FTRVAKYGLPILVTNDQSLKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVF
DVTTDIPKEGEAPRQKPEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAV
PQKWEESDPKFITNSQQVKLRSFTTTIHKVESMVAYKISKIKIIK*in
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllevwfyqlfktniikcklq*hlkqifh*kvqqrl*lnffhiv*iqfyfkedyihqn
hlqelqnmdyqyw*lminh*kii*imy*nnyqngy*vvmfknwf**slislqkrf*kdgy
smlqlifqkkekhqdknqkkr**mkfkqlldklqqvlhfyhfyqmhvhliy*yiqvki*q
ylkngknliqns*qihnklnldllqqqfiklkvwlpikfqrlk**nk*i


Frame B:
llaywrfgfinyskqi**nascssi*nkyfikrfnrdcn*iffi*ykynsiskriistri
iyksckiwitnign**siikrlfr*ciktiirmvikw*cskigfsnh*yrykrgfrkmgi
rcyn*yskrrrstktktrkrdne*nssny*tnyskcyifttftkcmyi*fisiyk*rfss
tskmgri*skihnkftts*t*ifynnns*s*kygcl*nfkd*nnkinky


Frame C:
CWPTGGLVLSTIQNKYNKMQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPES
FTRVAKYGLPILVTNDQSLKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVF
DVTTDIPKEGEAPRQKPEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAV
PQKWEESDPKFITNSQQVKLRSFTTTIHKVESMVAYKISKIKIIK*in
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10424-1 (Contig-U10424-1Q)
/CSM_Contig/Contig-U10424-1Q.Seq.d
         (688 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10424-1 (Contig-U10424-1Q) /CSM_Contig/Conti...  1241  0.0
Contig-U11233-1 (Contig-U11233-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U13504-1 (Contig-U13504-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U13023-1 (Contig-U13023-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U11908-1 (Contig-U11908-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U11133-1 (Contig-U11133-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U10847-1 (Contig-U10847-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U09420-1 (Contig-U09420-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U01375-1 (Contig-U01375-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U00742-1 (Contig-U00742-1Q) /CSM_Contig/Conti...    36  0.048

>Contig-U10424-1 (Contig-U10424-1Q) /CSM_Contig/Contig-U10424-1Q.Seq.d
          Length = 688

 Score = 1241 bits (626), Expect = 0.0
 Identities = 640/647 (98%)
 Strand = Plus / Plus

                                                                       
Query: 1   actgttggcctactggaggtttggttttatcaactattcaaaacaaatataataaaatgc 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   actgttggcctactggaggtttggttttatcaactattcaaaacaaatataataaaatgc 60

                                                                       
Query: 61  aagctgcagtagcatctaaaacaaatatttcattaaaaggttcaacagagattgtaactg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aagctgcagtagcatctaaaacaaatatttcattaaaaggttcaacagagattgtaactg 120

                                                                       
Query: 121 aannnnnnncatatagtataaatacaattctatttcaaagaggattatatccaccagaat 180
           ||       |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aatttttttcatatagtataaatacaattctatttcaaagaggattatatccaccagaat 180

                                                                       
Query: 181 catttacaagagttgcaaaatatggattaccaatattggtaactaatgatcaatcattaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 catttacaagagttgcaaaatatggattaccaatattggtaactaatgatcaatcattaa 240

                                                                       
Query: 241 aagattatttagataatgtattaaaacaattatcagaatggttattaagtggtgatgttc 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 aagattatttagataatgtattaaaacaattatcagaatggttattaagtggtgatgttc 300

                                                                       
Query: 301 aaaaattggttttagtaatcactgatatcgttacaaaagaggttttagaaagatgggtat 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 aaaaattggttttagtaatcactgatatcgttacaaaagaggttttagaaagatgggtat 360

                                                                       
Query: 361 tcgatgttacaactgatattccaaaagaaggagaagcaccaagacaaaaaccagaaaaag 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 tcgatgttacaactgatattccaaaagaaggagaagcaccaagacaaaaaccagaaaaag 420

                                                                       
Query: 421 agataatgaatgaaattcaagcaattattagacaaattacagcaagtgttacatttttac 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 agataatgaatgaaattcaagcaattattagacaaattacagcaagtgttacatttttac 480

                                                                       
Query: 481 cacttttaccaaatgcatgtacatttgatttattagtatatacaagtaaagatttagcag 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 cacttttaccaaatgcatgtacatttgatttattagtatatacaagtaaagatttagcag 540

                                                                       
Query: 541 tacctcaaaaatgggaagaatctgatccaaaattcataacaaattcacaacaagttaaac 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 tacctcaaaaatgggaagaatctgatccaaaattcataacaaattcacaacaagttaaac 600

                                                          
Query: 601 ttagatcttttacaacaacaattcataaagttgaaagtatggttgcc 647
           |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 ttagatcttttacaacaacaattcataaagttgaaagtatggttgcc 647


 Score = 32.2 bits (16), Expect = 0.75
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 207 ttaccaatattggtaa 222
           ||||||||||||||||
Sbjct: 222 ttaccaatattggtaa 207


>Contig-U11233-1 (Contig-U11233-1Q) /CSM_Contig/Contig-U11233-1Q.Seq.d
          Length = 2032

 Score = 38.2 bits (19), Expect = 0.012
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                               
Query: 522  acaagtaaagatttagcag 540
            |||||||||||||||||||
Sbjct: 1131 acaagtaaagatttagcag 1149


>Contig-U13504-1 (Contig-U13504-1Q) /CSM_Contig/Contig-U13504-1Q.Seq.d
          Length = 1197

 Score = 36.2 bits (18), Expect = 0.048
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                            
Query: 1  actgttggcctactggag 18
          ||||||||||||||||||
Sbjct: 2  actgttggcctactggag 19


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 11,062
Number of Sequences: 6905
Number of extensions: 11062
Number of successful extensions: 1633
Number of sequences better than 10.0: 541
length of query: 688
length of database: 5,674,871
effective HSP length: 16
effective length of query: 672
effective length of database: 5,564,391
effective search space: 3739270752
effective search space used: 3739270752
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.20
Homology vs DNA 
Query= Contig-U10424-1 (Contig-U10424-1Q) /CSM_Contig/Contig-U10424-1Q.Seq.d
         (688 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432301) Dictyostelium discoideum cDNA clone:ddv18e08, 3' ...  1029   0.0    2
(BJ414169) Dictyostelium discoideum cDNA clone:ddv18e08, 5' ...   874   0.0    2
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...   525   0.0    3
(FE413761) CBTU414.fwd CBTU_Daphnia_pulex_Chosen_One_Library...    58   5e-04  1
(FE413760) CBTU414.rev CBTU_Daphnia_pulex_Chosen_One_Library...    58   5e-04  1
(FE389514) CBTO2060.fwd CBTO_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE389513) CBTO2060.rev CBTO_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE372737) CBNP3311.fwd CBNP_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE372736) CBNP3311.rev CBNP_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE364082) CBNO3074.fwd CBNO_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE364081) CBNO3074.rev CBNO_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE346356) CBIB6575.fwd CBIB_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(FE346355) CBIB6575.rev CBIB_Daphnia_pulex_Chosen_One_Librar...    58   5e-04  1
(BU582709) rc67h08.y2 Meloidogyne hapla egg SL1 TOPO v1 Melo...    56   0.002  1
(BQ125410) rc67h08.y1 Meloidogyne hapla egg SL1 TOPO v1 Melo...    56   0.002  1
(BM884158) rc19h10.y1 Meloidogyne hapla egg pAMP1 v1 Meloido...    56   0.002  1
(BM883910) rc24b12.y1 Meloidogyne hapla egg pAMP1 v1 Meloido...    56   0.002  1
(BM883905) rc24b07.y1 Meloidogyne hapla egg pAMP1 v1 Meloido...    56   0.002  1
(AC181827) Strongylocentrotus purpuratus clone R3-1023B17, W...    40   0.004  5
(CP000361) Arcobacter butzleri RM4018, complete genome.            38   0.11   20
(CK426622) rx18a08.y1 Meloidogyne paranaensis egg SMART pGEM...    50   0.11   1
(EK365892) 1095469396744 Global-Ocean-Sampling_GS-31-01-01-1...    34   0.32   2
(DE979704) Macropus eugenii DNA, BAC clone: MEB1-187P15_R, 3...    34   0.35   3
(CP000382) Clostridium novyi NT, complete genome.                  40   0.35   18
(AC122785) Mus musculus chromosome 3, clone RP24-247G11, com...    48   0.44   1
(AC120055) Homo sapiens chromosome 18, clone CTD-2362J14, co...    48   0.44   1
(AC110014) Homo sapiens chromosome 18, clone CTC-782O7, comp...    48   0.44   1
(AC121078) Mus musculus clone RP24-373P24, LOW-PASS SEQUENCE...    48   0.44   1
(AC120883) Homo sapiens chromosome 18 clone CTD-2146H24 map ...    48   0.44   1
(AC020722) Homo sapiens chromosome 18 clone RP11-486C18, WOR...    48   0.44   1
(AZ446205) 1M0242I05R Mouse 10kb plasmid UUGC1M library Mus ...    48   0.44   1
(AZ095488) RPCI-23-474A9.TV RPCI-23 Mus musculus genomic clo...    48   0.44   1
(AG344360) Mus musculus molossinus DNA, clone:MSMg01-138M10....    48   0.44   1
(EH012744) USDA-FP_185614 Lysiphlebus testaceipes adult whol...    48   0.44   1
(CN573654) rc19h10.x1 Meloidogyne hapla egg pAMP1 v1 Meloido...    48   0.44   1
(CJ489536) Macaca fascicularis mRNA, clone: QtsA-17289, 5' e...    48   0.44   1
(AC116982) Dictyostelium discoideum chromosome 2 map 3622643...    38   0.48   9
(AL844185) Zebrafish DNA sequence from clone DKEY-30H22 in l...    38   0.50   9
(CR956392) M.truncatula DNA sequence from clone MTH2-52P12 o...    40   0.62   5
(AC108838) Mus musculus chromosome 18, clone RP23-326N14, co...    36   0.92   2
(BX537129) Zebrafish DNA sequence from clone CH211-236I20 in...    32   0.92   2
(CR759776) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    32   0.93   2
(CJ489087) Macaca fascicularis mRNA, clone: QtsA-16824, 5' e...    36   1.0    2
(CJ471514) Macaca fascicularis mRNA, clone: QorA-11190, 5' e...    32   1.3    3
(AL844507) Plasmodium falciparum chromosome 8.                     42   1.4    11
(AC004739) Homo sapiens PAC clone RP4-531G15 from 7p21, comp...    44   1.6    2
(L35321) Dictyostelium discoideum class VII unconventional m...    46   1.7    1
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    46   1.7    1
(AC128774) Rattus norvegicus clone CH230-440J1, WORKING DRAF...    46   1.7    1
(AC097399) Rattus norvegicus clone CH230-27I16, *** SEQUENCI...    46   1.7    1
(ER608008) 1093016289628 Global-Ocean-Sampling_GS-36-01-01-2...    46   1.7    1
(EJ552574) 1092959466101 Global-Ocean-Sampling_GS-29-01-01-1...    46   1.7    1
(EJ520202) 1092955132830 Global-Ocean-Sampling_GS-29-01-01-1...    46   1.7    1
(DU566451) OO__Ba0050H20.f OO__Ba Oryza officinalis genomic ...    46   1.7    1
(DH827321) Rattus norvegicus DNA, BAC clone: RNB1-433F13, 5'...    46   1.7    1
(CE649920) tigr-gss-dog-17000367355454 Dog Library Canis lup...    46   1.7    1
(CE265513) tigr-gss-dog-17000336940820 Dog Library Canis lup...    46   1.7    1
(EH369584) C07_j001_plate_115 j001 Nicotiana benthamiana cDN...    46   1.7    1
(AM840768) Nicotiana tabacum EST, clone nt002017057.               46   1.7    1
(CV021345) tbt_000690 Normalized Nicotiana tabacum cDNA libr...    46   1.7    1
(CV017227) tbt_000618 Normalized Nicotiana tabacum cDNA libr...    46   1.7    1
(CJ479440) Macaca fascicularis mRNA, clone: QtrA-14742, 5' e...    46   1.7    1
(CJ444660) Macaca fascicularis mRNA, clone: QccE-22268, 5' e...    46   1.7    1
(AW741038) uq94b03.y1 NCI_CGAP_Mam10 Mus musculus cDNA clone...    46   1.7    1
(CP000487) Campylobacter fetus subsp. fetus 82-40, complete ...    46   1.7    1
(DT323969) SusFleck_BF_0145_0148_5_pCNS_E22 SusFleck Backfat...    36   1.7    2
(BJ424664) Dictyostelium discoideum cDNA clone:ddv53l06, 5' ...    34   2.1    3
(BJ326680) Dictyostelium discoideum cDNA clone:dda17h15, 5' ...    36   2.2    2
(CP000942) Ureaplasma parvum serovar 3 str. ATCC 27815, comp...    32   2.4    15
(CP000728) Clostridium botulinum F str. Langeland, complete ...    36   2.6    19
(CI032757) Oryza sativa Japonica Group cDNA, clone: 009-M102...    36   2.6    2
(BJ329122) Dictyostelium discoideum cDNA clone:dda30i23, 5' ...    34   2.6    3
(AY916130) Epidermophyton floccosum mitochondrion, complete ...    32   2.9    5
(BJ326682) Dictyostelium discoideum cDNA clone:dda17i14, 5' ...    36   3.1    2
(FK124554) XABT164566.b1 Gateway compatible cien cDNA librar...    42   3.3    2
(CU468336) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    32   3.3    2
(CU693469) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    32   3.4    2
(AC231726) Monodelphis domestica clone VMRC18-269H24, WORKIN...    34   3.4    2
(FF690681) XABT103397.fwd Gateway compatible cien cDNA libra...    42   3.4    2
(FF719040) XABT36821.fwd Gateway compatible cien cDNA librar...    42   3.5    2
(CU582816) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    40   3.5    4
(CJ440278) Macaca fascicularis mRNA, clone: QccE-17588, 5' e...    42   3.6    2
(AC207864) Callicebus moloch clone LB5-319P22, WORKING DRAFT...    42   3.7    4
(CJ485063) Macaca fascicularis mRNA, clone: QtsA-12632, 5' e...    36   3.7    2
(CJ447254) Macaca fascicularis mRNA, clone: QflA-12576, 5' e...    34   3.8    2
(CJ455770) Macaca fascicularis mRNA, clone: QflA-21575, 5' e...    34   3.9    2
(AC189536) Brassica rapa subsp. pekinensis clone KBrH003D18,...    32   3.9    6
(CJ488419) Macaca fascicularis mRNA, clone: QtsA-16128, 5' e...    40   3.9    2
(FF718266) XABT36308.fwd Gateway compatible cien cDNA librar...    42   4.0    2
(EK216372) 1095460119833 Global-Ocean-Sampling_GS-31-01-01-1...    32   4.0    3
(FD935289) RS1GA21JQ RS1(AR) Raphanus sativus var. oleiformi...    32   4.5    2
(BX914777) Sus Scrofa library (scan) clone scan0028.l.15, 5p...    32   4.5    2
(AJ657779) Sus scrofa EST, clone C0005195_C20.                     32   4.5    2
(BJ415359) Dictyostelium discoideum cDNA clone:ddv22f12, 5' ...    32   4.5    2
(EW533243) rret12_k16.y1 ret Sus scrofa cDNA 5', mRNA sequence.    32   4.5    2
(BJ415695) Dictyostelium discoideum cDNA clone:ddv23l08, 5' ...    32   4.5    2
(FD939082) RS1GA21TF RS1(AR) Raphanus sativus var. oleiformi...    32   4.5    2
(EW619809) rsug26_b5.y1 sug Sus scrofa cDNA 5', mRNA sequence.     32   4.5    2
(EW195349) rpfco0137_c3.y1 fco Sus scrofa cDNA 5', mRNA sequ...    32   4.6    2
(BJ387300) Dictyostelium discoideum cDNA clone:dds1o12, 5' e...    34   4.6    2

>(BJ432301) Dictyostelium discoideum cDNA clone:ddv18e08, 3' end,
           single read.
          Length = 672

 Score = 1029 bits (519), Expect(2) = 0.0
 Identities = 519/519 (100%)
 Strand = Plus / Minus


Query: 130 catatagtataaatacaattctatttcaaagaggattatatccaccagaatcatttacaa 189
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 560 catatagtataaatacaattctatttcaaagaggattatatccaccagaatcatttacaa 501


Query: 190 gagttgcaaaatatggattaccaatattggtaactaatgatcaatcattaaaagattatt 249
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 500 gagttgcaaaatatggattaccaatattggtaactaatgatcaatcattaaaagattatt 441


Query: 250 tagataatgtattaaaacaattatcagaatggttattaagtggtgatgttcaaaaattgg 309
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 440 tagataatgtattaaaacaattatcagaatggttattaagtggtgatgttcaaaaattgg 381


Query: 310 ttttagtaatcactgatatcgttacaaaagaggttttagaaagatgggtattcgatgtta 369
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 380 ttttagtaatcactgatatcgttacaaaagaggttttagaaagatgggtattcgatgtta 321


Query: 370 caactgatattccaaaagaaggagaagcaccaagacaaaaaccagaaaaagagataatga 429
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 320 caactgatattccaaaagaaggagaagcaccaagacaaaaaccagaaaaagagataatga 261


Query: 430 atgaaattcaagcaattattagacaaattacagcaagtgttacatttttaccacttttac 489
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 260 atgaaattcaagcaattattagacaaattacagcaagtgttacatttttaccacttttac 201


Query: 490 caaatgcatgtacatttgatttattagtatatacaagtaaagatttagcagtacctcaaa 549
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 200 caaatgcatgtacatttgatttattagtatatacaagtaaagatttagcagtacctcaaa 141


Query: 550 aatgggaagaatctgatccaaaattcataacaaattcacaacaagttaaacttagatctt 609
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 140 aatgggaagaatctgatccaaaattcataacaaattcacaacaagttaaacttagatctt 81


Query: 610 ttacaacaacaattcataaagttgaaagtatggttgcct 648
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 80  ttacaacaacaattcataaagttgaaagtatggttgcct 42

 Score =  208 bits (105), Expect(2) = 0.0
 Identities = 105/105 (100%)
 Strand = Plus / Minus


Query: 18  ggtttggttttatcaactattcaaaacaaatataataaaatgcaagctgcagtagcatct 77
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 672 ggtttggttttatcaactattcaaaacaaatataataaaatgcaagctgcagtagcatct 613


Query: 78  aaaacaaatatttcattaaaaggttcaacagagattgtaactgaa 122
           |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 612 aaaacaaatatttcattaaaaggttcaacagagattgtaactgaa 568

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 843,874,342
Number of extensions: 51861323
Number of successful extensions: 4513062
Number of sequences better than 10.0: 162
Length of query: 688
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 664
Effective length of database: 97,308,875,965
Effective search space: 64613093640760
Effective search space used: 64613093640760
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10424-1 (Contig-U10424-1Q) /CSM_Contig/Contig-U10424-1Q.Seq.d
         (688 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556Y9) RecName: Full=Mitotic spindle assembly checkpoint prote...   389   e-107
(Q13257) RecName: Full=Mitotic spindle assembly checkpoint prote...   248   2e-64
AY890787_1(AY890787|pid:none) Synthetic construct Homo sapiens c...   248   2e-64
CP001331_306(CP001331|pid:none) Micromonas sp. RCC299 chromosome...   242   7e-63
U83902_1(U83902|pid:none) Mus musculus mitotic checkpoint compon...   242   9e-63
(Q9Z1B5) RecName: Full=Mitotic spindle assembly checkpoint prote...   242   9e-63
BC068714_1(BC068714|pid:none) Xenopus laevis hypothetical protei...   240   3e-62
BC045227_1(BC045227|pid:none) Xenopus laevis MAD2 (mitotic arres...   239   7e-62
BT081560_1(BT081560|pid:none) Rana catesbeiana clone rcat-evr-53...   238   2e-61
BT046714_1(BT046714|pid:none) Salmo salar clone ssal-rgb2-630-19...   237   2e-61
AM270199_61(AM270199|pid:none) Aspergillus niger contig An09c010...   214   2e-54
CP000600_201(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   212   1e-53
AB166871_1(AB166871|pid:none) Triticum aestivum wMAD2-A1 gene fo...   210   3e-53
AB166872_1(AB166872|pid:none) Triticum aestivum wMAD2-B1 gene fo...   210   4e-53
AB166873_1(AB166873|pid:none) Triticum aestivum wMAD2-D1 gene fo...   208   1e-52
AK111976_1(AK111976|pid:none) Oryza sativa Japonica Group cDNA c...   206   5e-52
BT076125_1(BT076125|pid:none) Caligus rogercresseyi clone crog-e...   206   7e-52
AE017343_149(AE017343|pid:none) Cryptococcus neoformans var. neo...   204   2e-51
AP007167_532(AP007167|pid:none) Aspergillus oryzae RIB40 genomic...   203   3e-51
(Q9XFH3) RecName: Full=Mitotic spindle checkpoint protein MAD2; ...   202   8e-51
BT078614_1(BT078614|pid:none) Lepeophtheirus salmonis clone lsal...   200   4e-50
AL606998_14(AL606998|pid:none) Oryza sativa genomic DNA, chromos...   199   8e-50
AY814258_1(AY814258|pid:none) Schistosoma japonicum SJCHGC08975 ...   198   1e-49
AM920428_1519(AM920428|pid:none) Penicillium chrysogenum Wiscons...   198   1e-49
(O14417) RecName: Full=Mitotic spindle checkpoint component mad2...   194   3e-48
AM488489_2(AM488489|pid:none) Vitis vinifera contig VV78X221559....   192   1e-47
AC084158_32(AC084158|pid:none) Caenorhabditis elegans cosmid Y69...   190   4e-47
CU633454_18(CU633454|pid:none) Podospora anserina genomic DNA ch...   189   7e-47
CR382131_564(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   189   7e-47
CR380956_41(CR380956|pid:none) Candida glabrata strain CBS138 ch...   181   2e-44
AE014296_1082(AE014296|pid:none) Drosophila melanogaster chromos...   180   3e-44
CT005264_173(CT005264|pid:none) Leishmania major strain Friedlin...   177   2e-43
AE016816_248(AE016816|pid:none) Ashbya gossypii (= Eremothecium ...   177   3e-43
CU928173_452(CU928173|pid:none) Zygosaccharomyces rouxii strain ...   172   8e-42
AM494962_233(AM494962|pid:none) Leishmania braziliensis chromoso...   171   1e-41
CU928171_318(CU928171|pid:none) Kluyveromyces thermotolerans str...   171   2e-41
AC105379_17(AC105379|pid:none) Trypanosoma brucei chromosome 3 c...   170   3e-41
CR382124_387(CR382124|pid:none) Kluyveromyces lactis strain NRRL...   165   1e-39
(P40958) RecName: Full=Mitotic spindle checkpoint component MAD2...   160   3e-38
CR382136_713(CR382136|pid:none) Debaryomyces hansenii strain CBS...   157   3e-37
BC110147_1(BC110147|pid:none) Bos taurus hypothetical LOC780876,...   155   1e-36
FM992688_379(FM992688|pid:none) Candida dubliniensis CD36 chromo...   145   8e-34
AK008638_1(AK008638|pid:none) Mus musculus adult male stomach cD...   127   2e-28
AC084158_33(AC084158|pid:none) Caenorhabditis elegans cosmid Y69...   106   6e-22
EU969714_1(EU969714|pid:none) Zea mays clone 334623 hypothetical...    86   8e-16
(Q0E7J8) RecName: Full=DNA polymerase zeta processivity subunit;...    65   2e-09
AL031731_9(AL031731|pid:none) Human DNA sequence from clone RP3-...    62   2e-08
AY675170_1(AY675170|pid:none) Gallus gallus REV7 (REV7) mRNA, co...    62   2e-08
AK009587_1(AK009587|pid:none) Mus musculus adult male tongue cDN...    61   4e-08
AL606929_10(AL606929|pid:none) Mouse DNA sequence from clone RP2...    61   4e-08
AL606929_11(AL606929|pid:none) Mouse DNA sequence from clone RP2...    61   4e-08
BT080023_1(BT080023|pid:none) Esox lucius clone eluc-evq-540-030...    60   5e-08
AL031731_11(AL031731|pid:none) Human DNA sequence from clone RP3...    60   5e-08
BC112559_1(BC112559|pid:none) Bos taurus MAD2 mitotic arrest def...    60   5e-08
(Q9UI95) RecName: Full=Mitotic spindle assembly checkpoint prote...    60   5e-08
AF080398_1(AF080398|pid:none) Homo sapiens MAD2-like protein mRN...    59   1e-07
BT075419_1(BT075419|pid:none) Osmerus mordax clone omor-eva-502-...    59   2e-07
BT033058_1(BT033058|pid:none) Drosophila melanogaster IP20312 fu...    56   9e-07
AM270347_57(AM270347|pid:none) Aspergillus niger contig An15c020...    56   1e-06
DQ240288_1(DQ240288|pid:none) Neurospora crassa REV7 (mus26) gen...    54   4e-06
BC087687_1(BC087687|pid:none) Rattus norvegicus MAD2 mitotic arr...    53   7e-06
AP007161_189(AP007161|pid:none) Aspergillus oryzae RIB40 genomic...    53   7e-06
AM920436_227(AM920436|pid:none) Penicillium chrysogenum Wisconsi...    53   1e-05
DQ213810_1(DQ213810|pid:none) Taeniopygia guttata clone 0061P002...    53   1e-05
(Q54S00) RecName: Full=Mitotic spindle assembly checkpoint prote...    51   4e-05
AB089496_1(AB089496|pid:none) Neurospora crassa mus-26 gene, com...    51   4e-05
DQ185032_1(DQ185032|pid:none) Emericella nidulans RevA mRNA, com...    49   1e-04
BC074654_1(BC074654|pid:none) Xenopus tropicalis MAD2 mitotic ar...    49   1e-04
CP001577_402(CP001577|pid:none) Micromonas sp. RCC299 chromosome...    44   0.005
AE016818_759(AE016818|pid:none) Ashbya gossypii (= Eremothecium ...    40   0.086
AF372829_1(AF372829|pid:none) Arabidopsis thaliana putative tran...    39   0.19
(P38927) RecName: Full=DNA polymerase zeta processivity subunit;...    37   0.55
CP000814_944(CP000814|pid:none) Campylobacter jejuni subsp. jeju...    37   0.72
CP000682_722(CP000682|pid:none) Metallosphaera sedula DSM 5348, ...    37   0.72
CP000025_1053(CP000025|pid:none) Campylobacter jejuni RM1221, co...    36   1.2
AL031731_12(AL031731|pid:none) Human DNA sequence from clone RP3...    36   1.2
AL111168_946(AL111168|pid:none) Campylobacter jejuni subsp. jeju...    36   1.2
AL031731_13(AL031731|pid:none) Human DNA sequence from clone RP3...    35   2.1
AE005673_1168(AE005673|pid:none) Caulobacter crescentus CB15, co...    35   2.1
CP000768_639(CP000768|pid:none) Campylobacter jejuni subsp. doyl...    35   2.8
AE006641_1993(AE006641|pid:none) Sulfolobus solfataricus P2, com...    35   2.8
CP001131_153(CP001131|pid:none) Anaeromyxobacter sp. K, complete...    34   3.6
CP000628_2065(CP000628|pid:none) Agrobacterium radiobacter K84 c...    34   4.7
CP001400_2593(CP001400|pid:none) Sulfolobus islandicus M.14.25, ...    34   4.7
CP000568_1719(CP000568|pid:none) Clostridium thermocellum ATCC 2...    34   4.7
CR380958_361(CR380958|pid:none) Candida glabrata strain CBS138 c...    34   4.7
CP000153_775(CP000153|pid:none) Sulfurimonas denitrificans DSM 1...    34   4.7
FN357316_45(FN357316|pid:none) Schistosoma mansoni genome sequen...    34   4.7
(Q8PZ69) RecName: Full=Putative adenylate kinase;          EC=2....    33   8.0

>(Q556Y9) RecName: Full=Mitotic spindle assembly checkpoint protein
           MAD2A; AltName: Full=MAD2-like 1;
           &AC116984_167(AC116984|pid:none)
          Length = 203

 Score =  389 bits (999), Expect = e-107
 Identities = 198/198 (100%), Positives = 198/198 (100%)
 Frame = +3

Query: 57  MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 236
           MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 237 LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIPKEGEAPRQKPE 416
           LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIPKEGEAPRQKPE
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIPKEGEAPRQKPE 120

Query: 417 KEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQV 596
           KEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQV
Sbjct: 121 KEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQV 180

Query: 597 KLRSFTTTIHKVESMVAY 650
           KLRSFTTTIHKVESMVAY
Sbjct: 181 KLRSFTTTIHKVESMVAY 198

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 933,503,959
Number of extensions: 16772012
Number of successful extensions: 44271
Number of sequences better than 10.0: 89
Number of HSP's gapped: 44198
Number of HSP's successfully gapped: 91
Length of query: 229
Length of database: 1,040,966,779
Length adjustment: 124
Effective length of query: 105
Effective length of database: 643,635,439
Effective search space: 67581721095
Effective search space used: 67581721095
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.91  gvh: 0.36  alm: 0.37  top: 0.67  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for Contig-U10424-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0