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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10396-1
Contig ID Contig-U10396-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Contig-U10396-1Q.Seq.d
GTTGGCCTACTGGNAATTTTAATATAACTCTAATATGTATTAAAATGAAA
TATTTAATAACATTACTATTTATATTCATTTTATTCAATTTTATAAATTC
AATAAATGCCTACAAAGATAGAGAAATAGTTAGAACTCATTTACAAACTA
TTATTGGTACTTCTGGTACAGTTGACAATCATGATATATCAACTAAATTA
GCACCAAGGATTTAAAAGGGATTTATCAAAAAGTTTATTAGCTGTGGATT
CAAAAATTAATGGTAAAAAGGTTGATATATTACCACATACACTCTATAGA
ATTTTATTTTCTTTTGATAATGATAAATTTAAAACTGGTTGGATTACAAT
TTGTGATGGAAATGGAACATATTTAAATCAAATCGATTTTTCATTCACTT
ATTCAAATGATAAAATTTTAGATTTAAAATGGCATTTAGATTATAATACA
GAAGAAGTTCATCATAAAAAGAAACCAAATAATTTTTATATAAATTATAA
ATGGAAAGAGATTAAGGATAAAGATATTTCAAGTGGATTGTTTATTTTAT
TTTTATTTGGAATTTTAATNNNNNNNNN
Gap no gap
Contig length 578
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 840899
End point 841469
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U10396
List of clone(s) 

est1=VFH657F,1,569
Translated Amino Acid sequence 
vgllxili*l*yvlk*ni**hyylysfysil*iq*mptkiek*leliyklllvllvqlti
miyqln*HQGFKRDLSKSLLAVDSKINGKKVDILPHTLYRILFSFDNDKFKTGWITICDG
NGTYLNQIDFSFTYSNDKILDLKWHLDYNTEEVHHKKKPNNFYINYKWKEIKDKDISSGL
FILFLFGILXXX
Translated Amino Acid sequence (All Frames) 
Frame A:
vgllxili*l*yvlk*ni**hyylysfysil*iq*mptkiek*leliyklllvllvqlti
miyqln*HQGFKRDLSKSLLAVDSKINGKKVDILPHTLYRILFSFDNDKFKTGWITICDG
NGTYLNQIDFSFTYSNDKILDLKWHLDYNTEEVHHKKKPNNFYINYKWKEIKDKDISSGL
FILFLFGILXXX


Frame B:
laywxf*ynsnmy*neifnnitiyihfiqfykfnkclqr*rns*nsftnyywyfwys*qs
*yin*istkdlkgiyqkvy*lwiqklmvkrliyyhihsiefyflliminlklvglqfvme
mehi*iksifhsliqmikf*i*ngi*iiiqkkfiikrnqiifi*iingkrlrikifqvdc
lfyfylef*xxx


Frame C:
wptgnfnitlicikmkylitllfifilfnfinsinaykdreivrthlqtiigtsgtvdnh
distklapri*kgfikkfiscgfkn*w*kg*yittytl*nfiff****i*nwldynl*wk
wnifksnrffihlfk**nfrfkmafrl*yrrsss*ketk*flykl*merd*g*ryfkwiv
yfifiwnfnxxx
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10396-1 (Contig-U10396-1Q)
/CSM_Contig/Contig-U10396-1Q.Seq.d
         (578 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Conti...  1065  0.0
Contig-U04416-1 (Contig-U04416-1Q) /CSM_Contig/Conti...    40  0.003
Contig-U11267-1 (Contig-U11267-1Q) /CSM_Contig/Conti...    38  0.010
Contig-U05695-1 (Contig-U05695-1Q) /CSM_Contig/Conti...    38  0.010
Contig-U14458-1 (Contig-U14458-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U13873-1 (Contig-U13873-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U12194-1 (Contig-U12194-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U11983-1 (Contig-U11983-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U10406-1 (Contig-U10406-1Q) /CSM_Contig/Conti...    36  0.040
Contig-U09402-1 (Contig-U09402-1Q) /CSM_Contig/Conti...    36  0.040

>Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Contig-U10396-1Q.Seq.d
          Length = 578

 Score = 1065 bits (537), Expect = 0.0
 Identities = 540/540 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   gttggcctactggnaattttaatataactctaatatgtattaaaatgaaatatttaataa 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   gttggcctactggnaattttaatataactctaatatgtattaaaatgaaatatttaataa 60

                                                                       
Query: 61  cattactatttatattcattttattcaattttataaattcaataaatgcctacaaagata 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  cattactatttatattcattttattcaattttataaattcaataaatgcctacaaagata 120

                                                                       
Query: 121 gagaaatagttagaactcatttacaaactattattggtacttctggtacagttgacaatc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gagaaatagttagaactcatttacaaactattattggtacttctggtacagttgacaatc 180

                                                                       
Query: 181 atgatatatcaactaaattagcaccaaggatttaaaagggatttatcaaaaagtttatta 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atgatatatcaactaaattagcaccaaggatttaaaagggatttatcaaaaagtttatta 240

                                                                       
Query: 241 gctgtggattcaaaaattaatggtaaaaaggttgatatattaccacatacactctataga 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gctgtggattcaaaaattaatggtaaaaaggttgatatattaccacatacactctataga 300

                                                                       
Query: 301 attttattttcttttgataatgataaatttaaaactggttggattacaatttgtgatgga 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attttattttcttttgataatgataaatttaaaactggttggattacaatttgtgatgga 360

                                                                       
Query: 361 aatggaacatatttaaatcaaatcgatttttcattcacttattcaaatgataaaatttta 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 aatggaacatatttaaatcaaatcgatttttcattcacttattcaaatgataaaatttta 420

                                                                       
Query: 421 gatttaaaatggcatttagattataatacagaagaagttcatcataaaaagaaaccaaat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 gatttaaaatggcatttagattataatacagaagaagttcatcataaaaagaaaccaaat 480

                                                                       
Query: 481 aatttttatataaattataaatggaaagagattaaggataaagatatttcaagtggattg 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 aatttttatataaattataaatggaaagagattaaggataaagatatttcaagtggattg 540


>Contig-U04416-1 (Contig-U04416-1Q) /CSM_Contig/Contig-U04416-1Q.Seq.d
          Length = 837

 Score = 40.1 bits (20), Expect = 0.003
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                   
Query: 42  aaaatgaaatatttaataacatta 65
           |||||||||| |||||||||||||
Sbjct: 118 aaaatgaaatttttaataacatta 141


>Contig-U11267-1 (Contig-U11267-1Q) /CSM_Contig/Contig-U11267-1Q.Seq.d
          Length = 1604

 Score = 38.2 bits (19), Expect = 0.010
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 1  gttggcctactggnaattttaa 22
          ||||||||||||||||||||||
Sbjct: 4  gttggcctactggnaattttaa 25


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 14,600
Number of Sequences: 6905
Number of extensions: 14600
Number of successful extensions: 2231
Number of sequences better than 10.0: 646
length of query: 578
length of database: 5,674,871
effective HSP length: 16
effective length of query: 562
effective length of database: 5,564,391
effective search space: 3127187742
effective search space used: 3127187742
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.11.19
Homology vs DNA 
Query= Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Contig-U10396-1Q.Seq.d
         (578 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ413643) Dictyostelium discoideum cDNA clone:ddv16a16, 5' ...  1065   0.0    1
(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...    38   2e-04  11
(AP009180) Candidatus Carsonella ruddii PV DNA, complete gen...    38   3e-04  9
(AL360354) Plasmodium vivax telomeric YAC clone, complete fi...    34   0.001  8
(AC005139) Plasmodium falciparum chromosome 12, *** SEQUENCI...    36   0.002  9
(CP000013) Borrelia garinii PBi, complete genome.                  36   0.002  15
(AL844509) Plasmodium falciparum chromosome 13.                    38   0.003  14
(CP000263) Buchnera aphidicola str. Cc (Cinara cedri), compl...    34   0.005  11
(AE014817) Plasmodium falciparum 3D7 chromosome 14 section 2...    36   0.005  12
(AC098858) Homo sapiens BAC clone RP11-149A7 from 4, complet...    40   0.005  6
(CP000361) Arcobacter butzleri RM4018, complete genome.            38   0.006  16
(AE000783) Borrelia burgdorferi B31, complete genome.              36   0.006  14
(AE014829) Plasmodium falciparum 3D7 chromosome 10 section 1...    34   0.007  11
(AL929356) Plasmodium falciparum strain 3D7, chromosome 9; s...    44   0.007  11
(CR382398) Plasmodium falciparum chromosome 6, complete sequ...    36   0.008  12
(AE001407) Plasmodium falciparum 3D7 chromosome 2 section 44...    40   0.008  4
(AF250284) Amsacta moorei entomopoxvirus, complete genome.         36   0.009  9
(AE014832) Plasmodium falciparum 3D7 chromosome 10 section 4...    32   0.009  11
(DB932347) Manihot esculenta mRNA, clone: CAS01_034_P23, 5'end.    50   0.009  2
(AL139295) Human chromosome 14 DNA sequence BAC R-61A17 of l...    42   0.010  6
(EK428579) 1095516061487 Global-Ocean-Sampling_GS-31-01-01-1...    50   0.015  2
(CU469464) Candidatus Phytoplasma mali strain AT complete ch...    34   0.016  15
(CU915525) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   0.018  5
(AE014816) Plasmodium falciparum 3D7 chromosome 14 section 1...    34   0.020  11
(AC218349) Bos taurus clone CH240-286E3, WORKING DRAFT SEQUE...    52   0.022  1
(ER538789) 1093015892092 Global-Ocean-Sampling_GS-35-01-01-1...    52   0.022  1
(AC025157) Homo sapiens 12 BAC RP11-401I10 (Roswell Park Can...    38   0.024  6
(AM465042) Vitis vinifera, whole genome shotgun sequence, co...    40   0.024  4
(AC068575) Homo sapiens chromosome 2 clone RP11-337J16 map 2...    38   0.024  8
(AL929353) Plasmodium falciparum strain 3D7, chromosome 5, s...    38   0.025  11
(AC214254) Heliconius erato clone BBAM-19O6, WORKING DRAFT S...    36   0.032  8
(CR382400) Plasmodium falciparum chromosome 6, complete sequ...    36   0.033  10
(AE014850) Plasmodium falciparum 3D7 chromosome 12, section ...    36   0.040  9
(AM638641) Entamoeba dispar GSS, clone dispar54e04.p1k.            48   0.041  2
(AC189492) Brassica rapa subsp. pekinensis clone KBrB084M08,...    34   0.046  7
(CP001056) Clostridium botulinum B str. Eklund 17B, complete...    38   0.047  16
(AC199750) Heliconius erato clone BBAM-27D18, WORKING DRAFT ...    36   0.052  8
(AE014824) Plasmodium falciparum 3D7 chromosome 14 section 9...    30   0.056  11
(AE014831) Plasmodium falciparum 3D7 chromosome 10 section 3...    34   0.056  11
(AP006852) Candida albicans genomic DNA, chromosome 7, compl...    38   0.068  14
(CP001078) Clostridium botulinum E3 str. Alaska E43, complet...    32   0.069  17
(EV431093) 210802367 NVP Nasonia vitripennis cDNA clone 195C...    32   0.075  3
(AM477892) Vitis vinifera contig VV78X273642.3, whole genome...    40   0.076  2
(BX537288) Zebrafish DNA sequence from clone DKEY-264B2 in l...    50   0.089  1
(AM426565) Vitis vinifera contig VV78X260215.10, whole genom...    50   0.089  1
(AC141812) Apis mellifera clone CH224-61C5, WORKING DRAFT SE...    50   0.089  1
(CL596469) OB__Ba0058A05.f OB__Ba Oryza brachyantha genomic ...    50   0.089  1
(CL542744) OB__Ba0067M21.f OB__Ba Oryza brachyantha genomic ...    50   0.089  1
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...    34   0.090  10
(CR548612) Paramecium tetraurelia macronuclear largest chrom...    34   0.098  15
(CP000942) Ureaplasma parvum serovar 3 str. ATCC 27815, comp...    30   0.10   16
(AE014845) Plasmodium falciparum 3D7 chromosome 12, section ...    42   0.11   9
(CP000123) Mycoplasma capricolum subsp. capricolum ATCC 2734...    32   0.12   16
(AM436652) Vitis vinifera contig VV78X145382.2, whole genome...    38   0.12   3
(AL031745) Plasmodium falciparum DNA from MAL1P2.                  32   0.12   12
(AL049183) Plasmodium falciparum DNA *** SEQUENCING IN PROGR...    38   0.13   8
(BY911375) Cryptomeria japonica cDNA clone: CMFL045_A24, 3'e...    32   0.13   3
(AC073229) Homo sapiens chromosome 4 clone RP11-660M4, *** S...    38   0.13   6
(CU179680) Mycoplasma agalactiae PG2 chromosome, complete se...    40   0.13   11
(DW281363) UI-S-GU0-adr-i-10-0-UI.s1 UI-S-GU0 Euprymna scolo...    42   0.14   2
(EK525221) 1095515639440 Global-Ocean-Sampling_GS-32-01-01-1...    34   0.15   3
(CT956007) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    36   0.16   8
(AF063866) Melanoplus sanguinipes entomopoxvirus, complete g...    34   0.17   10
(BX293980) Mycoplasma mycoides subsp. mycoides SC str. PG1, ...    34   0.17   13
(AP006628) Onion yellows phytoplasma OY-M DNA, complete genome.    36   0.18   13
(AY653733) Acanthamoeba polyphaga mimivirus, complete genome.      34   0.18   14
(CZ535170) SRAA-aac95f06.g1 Strongyloides ratti whole genome...    46   0.19   2
(CP000776) Campylobacter hominis ATCC BAA-381, complete genome.    34   0.21   16
(CU655857) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    36   0.22   7
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...    40   0.24   7
(CU928194) S.lycopersicum DNA sequence *** SEQUENCING IN PRO...    34   0.24   7
(AZ672256) ENTLL56TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(BH147331) ENTPW45TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(AZ532575) ENTCF66TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(AZ549737) ENTDE09TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(AZ534859) ENTBZ91TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(AZ535333) ENTCS42TR Entamoeba histolytica Sheared DNA Entam...    36   0.26   2
(BX927399) Zebrafish DNA sequence from clone DKEY-35A22 in l...    42   0.27   5
(AF160864) Tetrahymena pyriformis mitochondrial DNA, complet...    36   0.27   6
(AC006283) Arabidopsis thaliana chromosome 2 clone T1B3 map ...    34   0.28   7
(CR382399) Plasmodium falciparum chromosome 6, complete sequ...    38   0.28   9
(AC173250) Bos taurus clone CH240-527D7, WORKING DRAFT SEQUE...    36   0.29   8
(BA000021) Wigglesworthia glossinidia endosymbiont of Glossi...    36   0.33   16
(AL603754) Human DNA sequence from clone RP13-9P16 on chromo...    36   0.33   4
(BH149223) ENTPF92TR Entamoeba histolytica Sheared DNA Entam...    48   0.35   1
(AZ680829) ENTHE54TR Entamoeba histolytica Sheared DNA Entam...    48   0.35   1
(AZ679632) ENTJV56TF Entamoeba histolytica Sheared DNA Entam...    48   0.35   1
(FH254546) CHO_OF3221xk14r1.ab1 CHO_OF3 Nicotiana tabacum ge...    48   0.35   1
(AM665861) Entamoeba terrapinae GSS, clone terra019h01.p1k.        48   0.35   1
(DU558695) OO__Ba0039N20.r OO__Ba Oryza officinalis genomic ...    48   0.35   1
(CU135722) Equus caballus GSS, BAC clone CH241-258E4, T7 end...    48   0.35   1
(CU024416) Equus caballus GSS, BAC clone CH241-95F20, T7 end...    48   0.35   1
(BM415521) OP20597 Mixed Stage EST's from Globodera pallida,...    48   0.35   1
(AL160273) Human DNA sequence from clone RP11-366I5 on chrom...    40   0.36   6
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    32   0.37   8
(AM454860) Vitis vinifera contig VV78X113134.13, whole genom...    42   0.38   3
(AE014837) Plasmodium falciparum 3D7 chromosome 11 section 2...    36   0.39   9
(AC010908) Homo sapiens BAC clone CTD-3073A19 from 7, comple...    38   0.39   5
(L77117) Methanocaldococcus jannaschii DSM 2661, complete ge...    34   0.40   16
(BX927126) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    46   0.44   3

>(BJ413643) Dictyostelium discoideum cDNA clone:ddv16a16, 5' end,
           single read.
          Length = 569

 Score = 1065 bits (537), Expect = 0.0
 Identities = 540/540 (100%)
 Strand = Plus / Plus


Query: 1   gttggcctactggnaattttaatataactctaatatgtattaaaatgaaatatttaataa 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   gttggcctactggnaattttaatataactctaatatgtattaaaatgaaatatttaataa 60


Query: 61  cattactatttatattcattttattcaattttataaattcaataaatgcctacaaagata 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  cattactatttatattcattttattcaattttataaattcaataaatgcctacaaagata 120


Query: 121 gagaaatagttagaactcatttacaaactattattggtacttctggtacagttgacaatc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gagaaatagttagaactcatttacaaactattattggtacttctggtacagttgacaatc 180


Query: 181 atgatatatcaactaaattagcaccaaggatttaaaagggatttatcaaaaagtttatta 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atgatatatcaactaaattagcaccaaggatttaaaagggatttatcaaaaagtttatta 240


Query: 241 gctgtggattcaaaaattaatggtaaaaaggttgatatattaccacatacactctataga 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gctgtggattcaaaaattaatggtaaaaaggttgatatattaccacatacactctataga 300


Query: 301 attttattttcttttgataatgataaatttaaaactggttggattacaatttgtgatgga 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attttattttcttttgataatgataaatttaaaactggttggattacaatttgtgatgga 360


Query: 361 aatggaacatatttaaatcaaatcgatttttcattcacttattcaaatgataaaatttta 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 aatggaacatatttaaatcaaatcgatttttcattcacttattcaaatgataaaatttta 420


Query: 421 gatttaaaatggcatttagattataatacagaagaagttcatcataaaaagaaaccaaat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 gatttaaaatggcatttagattataatacagaagaagttcatcataaaaagaaaccaaat 480


Query: 481 aatttttatataaattataaatggaaagagattaaggataaagatatttcaagtggattg 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 aatttttatataaattataaatggaaagagattaaggataaagatatttcaagtggattg 540

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 878,710,726
Number of extensions: 60952596
Number of successful extensions: 5896057
Number of sequences better than 10.0: 671
Length of query: 578
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 555
Effective length of database: 93,106,754,628
Effective search space: 51674248818540
Effective search space used: 51674248818540
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Contig-U10396-1Q.Seq.d
         (578 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001324_842(CP001324|pid:none) Micromonas sp. RCC299 chromosome...    38   0.23
AP009484_22(AP009484|pid:none) Macrococcus caseolyticus JCSC5402...    35   1.9
BT053519_1(BT053519|pid:none) Medicago truncatula clone MTYFP_FQ...    35   1.9
AY372822_1(AY372822|pid:none) Magnaporthe grisea linoleate diol ...    34   3.3
CP001205_615(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    34   3.3
AC144741_14(AC144741|pid:none) Oryza sativa (japonica cultivar-g...    34   3.3
EU959807_1(EU959807|pid:none) Zea mays clone 220081 unknown mRNA.      33   4.2
(Q03N65) RecName: Full=tRNA uridine 5-carboxymethylaminomethyl m...    33   5.5
CP000903_5132(CP000903|pid:none) Bacillus weihenstephanensis KBA...    33   7.2
CP000395_658(CP000395|pid:none) Borrelia afzelii PKo, complete g...    33   7.2
CP000557_2164(CP000557|pid:none) Geobacillus thermodenitrificans...    33   7.2
CP001185_282(CP001185|pid:none) Thermosipho africanus TCF52B, co...    32   9.5

>CP001324_842(CP001324|pid:none) Micromonas sp. RCC299 chromosome 3,
           complete sequence.
          Length = 173

 Score = 37.7 bits (86), Expect = 0.23
 Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 12/89 (13%)
 Frame = +1

Query: 295 YRILFSFDNDKFKTGWITICDGNGTYLNQIDFSFTYSNDKILD------------LKWHL 438
           Y+I  SF+ D+  TGW+   D +   +  +D   T+   +I              LK H
Sbjct: 75  YKISLSFEGDRHLTGWLLRGDEDPGVVPMLDIEITHGRGEIRAVKADTVAVGRKYLKTHR 134

Query: 439 DYNTEEVHHKKKPNNFYINYKWKEIKDKD 525
               E  +H   P +  + Y+W E  D D
Sbjct: 135 SLVEEFHNHTVWPKHLLVRYRWTEKTDVD 163

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 760,172,252
Number of extensions: 14454649
Number of successful extensions: 43754
Number of sequences better than 10.0: 12
Number of HSP's gapped: 43741
Number of HSP's successfully gapped: 12
Length of query: 192
Length of database: 1,040,966,779
Length adjustment: 121
Effective length of query: 71
Effective length of database: 653,248,294
Effective search space: 46380628874
Effective search space used: 46380628874
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.68  gvh: 0.45  alm: 0.30  top: 0.47  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for Contig-U10396-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0