NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10335-1
Contig ID Contig-U10335-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10335-1 (Contig-U10335-1Q) /CSM_Contig/Contig-U10335-1Q.Seq.d
ATTTTTTTTCTAAATATATAAAAAATAATAATAATAATAATAATATAATA
ATAATATAATAATATATAAAGAAATATAAACAATAATATACATTAAAAAA
ACAATATATAAACAATAAACAATTAAAATAAAAAAAAAAAATTAAAATGA
CAAATACCAATAACTACGATGAAGAAAAAGATGAAGATGGAGAAAATGAT
GACTTATTAAATTATATTATAAATGGATTAATATTTGATATTGATAAATT
AGAAAAAGAGATTATTGAATTACAAGAAACTAAAACATCCACAACTCCAG
CTTCAACAACACCAACTTCAACAACACCAAGATCAAAATCCTCATCGGAC
TTACAACAATCAGAATCCCACCAACAACAACAACAACAACCACAAAATAA
ATCACAAACACCAAACTATAAAGAAGAATTAAATAATGTTACAAATTTAA
TAATACAAGAATTTGAACAATCTAAAATTTCATCATTAGCAACCAATAAT
AATATTAATAATAATAAGAGGATAACAATACCTGATAATCATTCAAATAA
TCCTGATAAACTATTAGAAATTCAATTAATTAATAAAATATTTGATATAA
GTAAAGCATTTGATGGTAAATCAAATAATTTAGTCTCCTCTTTTCAAAAT
TGTACAAATAATAATAATAATAATAATAATAACACTGACAACAACAACAA
TAACAATATTTCAAATAATAATAATAATAACAATGTTCCGACATTACAAC
CACTATCTTTTAATAATAGAAATAATTTAGTAAATGGTAATATTAGTAGC
AGCAGTAGTAGTAATAGTAGTAATAATAATATTGGTAGTAGTAATAGTAA
CAATGTAACAATAGGTAGTTTAAGGGATTTGAATAAAATTCAACCACAAA
GTTTAAATGAATTTGGTGAATGGAAATCATTAATTGATGAAGAATTATCA
GATCTCTATACATTGGTTAAAGAATTAAAACGTAAAATACCTTCAAATAC
TAGAACAACAAGTTCTAATAATATAACTGATTCTCCTGTGTCTAAAGGTA
GAAATAAAGATTTATATTTGTATAATAATACTTTTACAAACATTTTACAA
TTAAAAAATGATTTAGTATCAATGATCCAAAGTCCAATGTTCAAAGATAA
TGAATATAATAAACATAATATTGGTAGTGATTCATATAGTAGTAATAATA
ATAGTAATAATAATTTATCAAATCTTAAAGATGATGGAACCTCAACAAAT
ACAGCGGGTGGTGGTTCACCAAATAAAAAGAGTGTAAAACAATTAGTATA
TATTTTCCCCATAAAATGTAAACATAATAAAACAAAAGATAAAAATAAAA
ACA
Gap no gap
Contig length 1353
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 2769724
End point 2768368
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 6
Link to clone list U10335
List of clone(s) 

est1=VFK226F,1,139
est2=SSI342F,21,586
est3=VFH111F,152,745
est4=VFK226Z,571,1315
est5=VFH111Z,699,1303
est6=SSI342Z,794,1354
Translated Amino Acid sequence 
ffskyiknnnnnnniiii**yikkykq*ytlkkqyinnkqlk*KKKIKMTNTNNYDEEKD
EDGENDDLLNYIINGLIFDIDKLEKEIIELQETKTSTTPASTTPTSTTPRSKSSSDLQQS
ESHQQQQQQPQNKSQTPNYKEELNNVTNLIIQEFEQSKISSLATNNNINNNKRITIPDNH
SNNPDKLLEIQLINKIFDISKAFDGKSNNLVSSFQNCTNNNNNNNNNTDNNNNNNISNNN
NNNNVPTLQPLSFNNRNNLVNGNISSSSSSNSSNNNIGSSNSNNVTIGSLRDLNKIQPQS
LNEFGEWKSLIDEELSDLYTLVKELKRKIPSNTRTTSSNNITDSPVSKGRNKDLYLYNNT
FTNILQLKNDLVSMIQSPMFKDNEYNKHNIGSDSYSSNNNSNNNLSNLKDDGTSTNTAGG
GSPNKKSVKQLVYIFPIKCKHNKTKDKNKN
Translated Amino Acid sequence (All Frames) 
Frame A:
ifflni*kiiiiiii***ynni*rninnnih*knni*tinn*nkkkklk*qipittmkkk
mkmekmmty*iil*md*ylilin*kkrllnykklkhpqlqlqqhqlqqhqdqnphrtynn
qnptnnnnnnhkinhkhqtikkn*imlqi**yknlnnlkfhh*qpiiiliiirg*qylii
iqiiliny*kfn*likyli*vkhlmvnqii*splfkivqiiiiiiiitlttttitifqii
iiitmfrhynhylliieii**mvilvaavvvivviiilvvvivtm*q*vv*gi*ikfnhk
v*mnlvngnh*lmknyqisihwlkn*nvkylqileqqvlii*lillclkveikiyiciii
llqtfyn*kmi*yq*skvqcskimniiniilvvihivviiiviiiyqilkmmepqqiqrv
vvhqikrv*nn*yifsp*nvniikqkikikt


Frame B:
fff*iykk******ynnniiiykei*tiiyikktiykq*tikikkkn*ndkyq*lr*rkr
*rwrk**liklyykwini*y**irkrdy*itrn*nihnssfnntnfnntkikiligltti
ripptttttttk*itntkl*rrik*cykfnntri*ti*nfiisnq**y****ednnt**s
fk*s**tirnsin**ni*yk*si*w*ik*fsllfsklyk********h*qqqq*qyfk**
***qcsditttif***k*fskw*y**qq********yw*****qcnnr*fkgfe*nsttk
fk*iw*meiin**riirslyig*rikt*ntfky*nnkf**yn*fscv*r*k*rfifv**y
fykhftikk*fsindpksnvqr**i**t*yw**fi*********fiks*r*wnlnkysgw
wftk*kecktisiyfphkm*t**nkr*k*k


Frame C:
ffskyiknnnnnnniiii**yikkykq*ytlkkqyinnkqlk*KKKIKMTNTNNYDEEKD
EDGENDDLLNYIINGLIFDIDKLEKEIIELQETKTSTTPASTTPTSTTPRSKSSSDLQQS
ESHQQQQQQPQNKSQTPNYKEELNNVTNLIIQEFEQSKISSLATNNNINNNKRITIPDNH
SNNPDKLLEIQLINKIFDISKAFDGKSNNLVSSFQNCTNNNNNNNNNTDNNNNNNISNNN
NNNNVPTLQPLSFNNRNNLVNGNISSSSSSNSSNNNIGSSNSNNVTIGSLRDLNKIQPQS
LNEFGEWKSLIDEELSDLYTLVKELKRKIPSNTRTTSSNNITDSPVSKGRNKDLYLYNNT
FTNILQLKNDLVSMIQSPMFKDNEYNKHNIGSDSYSSNNNSNNNLSNLKDDGTSTNTAGG
GSPNKKSVKQLVYIFPIKCKHNKTKDKNKN
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10335-1 (Contig-U10335-1Q)
/CSM_Contig/Contig-U10335-1Q.Seq.d
         (1353 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10335-1 (Contig-U10335-1Q) /CSM_Contig/Conti...   587  e-167
Contig-U00621-1 (Contig-U00621-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U00425-1 (Contig-U00425-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U12028-1 (Contig-U12028-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U10739-1 (Contig-U10739-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U08437-1 (Contig-U08437-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U08110-1 (Contig-U08110-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U06696-1 (Contig-U06696-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U14885-1 (Contig-U14885-1Q) /CSM_Contig/Conti...    38  0.024
Contig-U12645-1 (Contig-U12645-1Q) /CSM_Contig/Conti...    38  0.024

>Contig-U10335-1 (Contig-U10335-1Q) /CSM_Contig/Contig-U10335-1Q.Seq.d
          Length = 1353

 Score =  587 bits (296), Expect = e-167
 Identities = 296/296 (100%)
 Strand = Plus / Plus

                                                                        
Query: 850  acaatgtaacaataggtagtttaagggatttgaataaaattcaaccacaaagtttaaatg 909
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 850  acaatgtaacaataggtagtttaagggatttgaataaaattcaaccacaaagtttaaatg 909

                                                                        
Query: 910  aatttggtgaatggaaatcattaattgatgaagaattatcagatctctatacattggtta 969
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 910  aatttggtgaatggaaatcattaattgatgaagaattatcagatctctatacattggtta 969

                                                                        
Query: 970  aagaattaaaacgtaaaataccttcaaatactagaacaacaagttctaataatataactg 1029
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 970  aagaattaaaacgtaaaataccttcaaatactagaacaacaagttctaataatataactg 1029

                                                                        
Query: 1030 attctcctgtgtctaaaggtagaaataaagatttatatttgtataataatacttttacaa 1089
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1030 attctcctgtgtctaaaggtagaaataaagatttatatttgtataataatacttttacaa 1089

                                                                    
Query: 1090 acattttacaattaaaaaatgatttagtatcaatgatccaaagtccaatgttcaaa 1145
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1090 acattttacaattaaaaaatgatttagtatcaatgatccaaagtccaatgttcaaa 1145


 Score =  446 bits (225), Expect = e-125
 Identities = 225/225 (100%)
 Strand = Plus / Plus

                                                                       
Query: 142 ttaaaatgacaaataccaataactacgatgaagaaaaagatgaagatggagaaaatgatg 201
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142 ttaaaatgacaaataccaataactacgatgaagaaaaagatgaagatggagaaaatgatg 201

                                                                       
Query: 202 acttattaaattatattataaatggattaatatttgatattgataaattagaaaaagaga 261
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 202 acttattaaattatattataaatggattaatatttgatattgataaattagaaaaagaga 261

                                                                       
Query: 262 ttattgaattacaagaaactaaaacatccacaactccagcttcaacaacaccaacttcaa 321
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 262 ttattgaattacaagaaactaaaacatccacaactccagcttcaacaacaccaacttcaa 321

                                                        
Query: 322 caacaccaagatcaaaatcctcatcggacttacaacaatcagaat 366
           |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 322 caacaccaagatcaaaatcctcatcggacttacaacaatcagaat 366


 Score =  208 bits (105), Expect = 1e-53
 Identities = 105/105 (100%)
 Strand = Plus / Plus

                                                                       
Query: 552 cctgataaactattagaaattcaattaattaataaaatatttgatataagtaaagcattt 611
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 552 cctgataaactattagaaattcaattaattaataaaatatttgatataagtaaagcattt 611

                                                        
Query: 612 gatggtaaatcaaataatttagtctcctcttttcaaaattgtaca 656
           |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 612 gatggtaaatcaaataatttagtctcctcttttcaaaattgtaca 656


 Score =  190 bits (96), Expect = 3e-48
 Identities = 96/96 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1215 ttatcaaatcttaaagatgatggaacctcaacaaatacagcgggtggtggttcaccaaat 1274
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1215 ttatcaaatcttaaagatgatggaacctcaacaaatacagcgggtggtggttcaccaaat 1274

                                                
Query: 1275 aaaaagagtgtaaaacaattagtatatattttcccc 1310
            ||||||||||||||||||||||||||||||||||||
Sbjct: 1275 aaaaagagtgtaaaacaattagtatatattttcccc 1310


 Score =  153 bits (77), Expect = 6e-37
 Identities = 77/77 (100%)
 Strand = Plus / Plus

                                                                       
Query: 417 tataaagaagaattaaataatgttacaaatttaataatacaagaatttgaacaatctaaa 476
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 417 tataaagaagaattaaataatgttacaaatttaataatacaagaatttgaacaatctaaa 476

                            
Query: 477 atttcatcattagcaac 493
           |||||||||||||||||
Sbjct: 477 atttcatcattagcaac 493


 Score = 61.9 bits (31), Expect = 2e-09
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 731 caatgttccgacattacaaccactatctttt 761
           |||||||||||||||||||||||||||||||
Sbjct: 731 caatgttccgacattacaaccactatctttt 761


>Contig-U00621-1 (Contig-U00621-1Q) /CSM_Contig/Contig-U00621-1Q.Seq.d
          Length = 605

 Score = 44.1 bits (22), Expect = 4e-04
 Identities = 31/34 (91%)
 Strand = Plus / Plus

                                             
Query: 297 ccagcttcaacaacaccaacttcaacaacaccaa 330
           ||||||||||   |||||||||||||||||||||
Sbjct: 333 ccagcttcaaatgcaccaacttcaacaacaccaa 366


>Contig-U00425-1 (Contig-U00425-1Q) /CSM_Contig/Contig-U00425-1Q.Seq.d
          Length = 664

 Score = 44.1 bits (22), Expect = 4e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                     
Query: 301 cttcaacaacaccaacttcaacaaca 326
           ||||||||||| ||||||||||||||
Sbjct: 469 cttcaacaacaacaacttcaacaaca 494


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 20,765
Number of Sequences: 6905
Number of extensions: 20765
Number of successful extensions: 2754
Number of sequences better than 10.0: 357
length of query: 1353
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1337
effective length of database: 5,564,391
effective search space: 7439590767
effective search space used: 7439590767
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.19
Homology vs DNA 
Query= Contig-U10335-1 (Contig-U10335-1Q) /CSM_Contig/Contig-U10335-1Q.Seq.d
         (1353 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...   339   0.0    9
(BJ433259) Dictyostelium discoideum cDNA clone:ddv21c08, 3' ...   549   0.0    5
(BJ431667) Dictyostelium discoideum cDNA clone:ddv15e03, 3' ...   549   0.0    3
(C90387) Dictyostelium discoideum slug cDNA, clone SSI342.        549   0.0    2
(BJ413311) Dictyostelium discoideum cDNA clone:ddv15e03, 5' ...   192   e-150  4
(AU073776) Dictyostelium discoideum slug cDNA, clone SSI342.      111   7e-20  1
(ET341403) S0283H24_SP6 CHORI-214 Salmo salar genomic clone ...    36   0.045  2
(EU005511) Libnotes perkinsi voucher O'Grady 200981 tRNA-Leu...    36   0.17   2
(Z93275) N.australis mitochondrial 12S rRNA gene (partial).        50   0.22   1
(CP001056) Clostridium botulinum B str. Eklund 17B, complete...    38   0.49   20
(AL929352) Plasmodium falciparum strain 3D7, chromosome 5, s...    38   0.56   10
(AC024600) Homo sapiens chromosome 10 clone RP11-179K3, comp...    38   0.62   4
(EU005510) Libnotes perkinsi voucher O'Grady 200947 tRNA-Leu...    34   0.63   2
(AC013686) Homo sapiens clone RP11-21A12, WORKING DRAFT SEQU...    48   0.74   2
(AC157922) Mus musculus chromosome 1 clone RP23-134J10 map 1...    46   0.83   4
(AB072298) Ipomoea nil transposon Tpn131 DNA, complete seque...    46   0.85   2
(AB132955) Homo sapiens DNA, STS on chromosome 7, D7S0542i, ...    48   0.88   1
(BX682533) Mouse DNA sequence from clone RP23-108L1 on chrom...    48   0.88   1
(DJ460685) Method for analysing genome using microsatellite ...    48   0.88   1
(CQ607976) Sequence 35734 from Patent WO0171042.                   48   0.88   1
(AE014297) Drosophila melanogaster chromosome 3R, complete s...    48   0.88   1
(AC007691) Drosophila melanogaster, chromosome 3R, region 82...    48   0.88   1
(AC007531) Drosophila melanogaster, chromosome 3R, region 82...    48   0.88   1
(AC116362) Homo sapiens chromosome 5 clone RP11-756H20, comp...    48   0.88   1
(AC094102) Homo sapiens chromosome 5 clone RP11-409A17, comp...    48   0.88   1
(AC073934) Human Chromosome 7 clone SCb-354C16, complete seq...    48   0.88   1
(AC010372) Homo sapiens chromosome 5 clone CTD-2049O16, comp...    48   0.88   1
(AC009181) Homo sapiens clone RP11-224A1 from 7q31, complete...    48   0.88   1
(AC008624) Homo sapiens chromosome 5 clone CTB-154E19, compl...    48   0.88   1
(AC091241) Mus musculus clone RP23-108L1, WORKING DRAFT SEQU...    48   0.88   1
(CR940352) Theileria annulata genomic DNA chromosome 3.            48   0.88   1
(AC024622) Homo sapiens clone RP11-756H20, WORKING DRAFT SEQ...    48   0.88   1
(AC014880) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    48   0.88   1
(EK462206) 1095469397124 Global-Ocean-Sampling_GS-32-01-01-1...    48   0.88   1
(AU271976) Dictyostelium discoideum gamete cDNA clone:FC-IC0...    48   0.88   1
(AJ928061) Theileria annulata EST, clone tam011e12_p1k.            48   0.88   1
(BM169017) EST571540 PyBS Plasmodium yoelii yoelii cDNA clon...    48   0.88   1
(BG860809) 1024073A02.y1 C. reinhardtii CC-1690, normalized,...    48   0.88   1
(FE670306) mh2_0026_E04 Chickpea leave library Cicer arietin...    48   0.88   1
(FE668918) mh2_0007_A07 Chickpea leave library Cicer arietin...    48   0.88   1
(EY329891) CAWY3062.rev CAWY Helobdella robusta Subtracted E...    36   0.91   2
(AE014831) Plasmodium falciparum 3D7 chromosome 10 section 3...    34   1.0    10
(AM487384) Vitis vinifera contig VV78X065901.3, whole genome...    38   1.4    6
(AC189566) Brassica rapa subsp. pekinensis clone KBrH009D02,...    38   1.5    6
(AP006628) Onion yellows phytoplasma OY-M DNA, complete genome.    36   1.6    2
(AC203454) Salmo salar clone CH214-70C22, WORKING DRAFT SEQU...    34   1.7    2
(AC204486) Salmo salar clone CH214-69I7, WORKING DRAFT SEQUE...    34   1.7    2
(AC204489) Salmo salar clone CH214-11O13, WORKING DRAFT SEQU...    34   1.8    2
(AC044784) Homo sapiens chromosome 10 clone RP11-428L9, comp...    36   1.8    2
(AL157784) Human DNA sequence *** SEQUENCING CANCELLED *** f...    36   1.8    2
(FF670686) G826P5387FC9.T0 Acorn worm normalized juvenile pE...    40   1.8    3
(AE014848) Plasmodium falciparum 3D7 chromosome 12, section ...    34   2.1    9
(ET420877) S0196G20_T7 CHORI-214 Salmo salar genomic clone S...    34   2.3    2
(ET329551) S0252H07_T7 CHORI-214 Salmo salar genomic clone S...    34   2.3    2
(AY347307) Penicillium marneffei strain MP1 mitochonrion, co...    44   2.4    2
(DY693647) EST_ssal_rgb2_49386 ssalrgb2 mixed_tissue Salmo s...    34   2.4    2
(EJ625846) 1092963164575 Global-Ocean-Sampling_GS-29-01-01-1...    38   2.7    3
(BQ597183) PfESToab35h11.y1 Plasmodium falciparum 3D7 asexua...    36   2.8    2
(EK037807) 1092959484497 Global-Ocean-Sampling_GS-31-01-01-1...    36   2.8    2
(EJ636174) 1092995301465 Global-Ocean-Sampling_GS-29-01-01-1...    38   3.0    3
(AC110897) Mus musculus chromosome 15, clone RP24-108J19, co...    46   3.5    1
(CR382132) Yarrowia lipolytica chromosome F of strain CLIB12...    46   3.5    1
(AX094620) Sequence 744 from Patent WO0118547.                     46   3.5    1
(AL034557) Plasmodium falciparum MAL4P1.                           46   3.5    1
(AJ635260) Phodaga marmorata 5.8S rRNA gene (partial), ITS2 ...    46   3.5    1
(AC010212) Drosophila melanogaster clone BACR32D05, complete...    46   3.5    1
(AC134185) Rattus norvegicus clone CH230-119J16, WORKING DRA...    46   3.5    1
(AC128226) Rattus norvegicus clone CH230-484E4, WORKING DRAF...    46   3.5    1
(AC123224) Rattus norvegicus clone CH230-186M22, *** SEQUENC...    46   3.5    1
(AC100403) Mus musculus clone RP23-134J9, LOW-PASS SEQUENCE ...    46   3.5    1
(AC097903) Rattus norvegicus clone CH230-146J6, *** SEQUENCI...    46   3.5    1
(AC218232) Bos taurus clone CH240-28C2, WORKING DRAFT SEQUEN...    46   3.5    1
(AC217098) Solanum tuberosum chromosome 6 clone RHPOTKEY044B...    46   3.5    1
(AC214515) Zea mays chromosome 1 clone ZMMBBb-228J19; ZMMBBb...    46   3.5    1
(AC205855) Zea mays chromosome 1 clone CH201-137E13; ZMMBBc0...    46   3.5    1
(AC190496) Zea mays chromosome 2 clone CH201-312N7; ZMMBBc03...    46   3.5    1
(AC183436) Asparagus officinalis clone 025-31L4, *** SEQUENC...    46   3.5    1
(AC181096) Strongylocentrotus purpuratus clone R3-3045G22, W...    46   3.5    1
(AC179257) Strongylocentrotus purpuratus clone R3-42M6, WORK...    46   3.5    1
(AC176862) Strongylocentrotus purpuratus clone R3-3120P4, WO...    46   3.5    1
(AC168554) Strongylocentrotus purpuratus clone R3-3091G16, W...    46   3.5    1
(AC168405) Strongylocentrotus purpuratus clone R3-4002F8, WO...    46   3.5    1
(AC014368) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    46   3.5    1
(AK158928) Mus musculus visual cortex cDNA, RIKEN full-lengt...    46   3.5    1
(FI007048) CHO_OF6900xi12f1.ab1 CHO_OF6 Nicotiana tabacum ge...    46   3.5    1
(FH543860) CHO_OF4749xk20r1.ab1 CHO_OF4 Nicotiana tabacum ge...    46   3.5    1
(FH230474) CHO_OF3407xm02r1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   3.5    1
(FH217499) CHO_OF3339xa14r1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   3.5    1
(ET712510) CHO_OF091xc02r1.ab1 CHO_OF Nicotiana tabacum geno...    46   3.5    1
(ET712445) CHO_OF091xc02f1.ab1 CHO_OF Nicotiana tabacum geno...    46   3.5    1
(EK149856) 1095456042950 Global-Ocean-Sampling_GS-31-01-01-1...    46   3.5    1
(DU713775) pyl49h11.b1 HK104 Caenorhabditis briggsae genomic...    46   3.5    1
(CZ481823) e04070-5prime Exelixis piggyBac RB insertions Dro...    46   3.5    1
(CW008131) ZMMBLa0010B24.r ZMMBLa Zea mays genomic clone ZMM...    46   3.5    1
(CC682927) OGUDD13TH ZM_0.7_1.5_KB Zea mays genomic clone ZM...    46   3.5    1
(BX230858) Danio rerio genomic clone DKEY-248D9, genomic sur...    46   3.5    1
(EB572980) AGENCOURT_51796569 D. virilis EST Drosophila viri...    46   3.5    1
(DY274420) IC0AAA36CE12RM1 CitNFL Citrus clementina cDNA 5',...    46   3.5    1
(DR911638) USDA-FP_19766 Citrus sinensis phloem Citrus sinen...    46   3.5    1
(AU186194) Citrus unshiu mRNA, clone:pcMOvA051405, expressed...    46   3.5    1

>(AC116984) Dictyostelium discoideum chromosome 2 map 2567470-3108875
              strain AX4, complete sequence.
          Length = 541399

 Score =  339 bits (171), Expect(9) = 0.0
 Identities = 171/171 (100%)
 Strand = Plus / Minus


Query: 870    ttaagggatttgaataaaattcaaccacaaagtttaaatgaatttggtgaatggaaatca 929
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526533 ttaagggatttgaataaaattcaaccacaaagtttaaatgaatttggtgaatggaaatca 526474


Query: 930    ttaattgatgaagaattatcagatctctatacattggttaaagaattaaaacgtaaaata 989
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526473 ttaattgatgaagaattatcagatctctatacattggttaaagaattaaaacgtaaaata 526414


Query: 990    ccttcaaatactagaacaacaagttctaataatataactgattctcctgtg 1040
              |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526413 ccttcaaatactagaacaacaagttctaataatataactgattctcctgtg 526363

 Score =  192 bits (97), Expect(9) = 0.0
 Identities = 97/97 (100%)
 Strand = Plus / Minus


Query: 560    actattagaaattcaattaattaataaaatatttgatataagtaaagcatttgatggtaa 619
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526843 actattagaaattcaattaattaataaaatatttgatataagtaaagcatttgatggtaa 526784


Query: 620    atcaaataatttagtctcctcttttcaaaattgtaca 656
              |||||||||||||||||||||||||||||||||||||
Sbjct: 526783 atcaaataatttagtctcctcttttcaaaattgtaca 526747

 Score =  170 bits (86), Expect(9) = 0.0
 Identities = 86/86 (100%)
 Strand = Plus / Minus


Query: 267    gaattacaagaaactaaaacatccacaactccagcttcaacaacaccaacttcaacaaca 326
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 527136 gaattacaagaaactaaaacatccacaactccagcttcaacaacaccaacttcaacaaca 527077


Query: 327    ccaagatcaaaatcctcatcggactt 352
              ||||||||||||||||||||||||||
Sbjct: 527076 ccaagatcaaaatcctcatcggactt 527051

 Score =  168 bits (85), Expect(9) = 0.0
 Identities = 85/85 (100%)
 Strand = Plus / Minus


Query: 1215   ttatcaaatcttaaagatgatggaacctcaacaaatacagcgggtggtggttcaccaaat 1274
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526009 ttatcaaatcttaaagatgatggaacctcaacaaatacagcgggtggtggttcaccaaat 525950


Query: 1275   aaaaagagtgtaaaacaattagtat 1299
              |||||||||||||||||||||||||
Sbjct: 525949 aaaaagagtgtaaaacaattagtat 525925

 Score =  155 bits (78), Expect(9) = 0.0
 Identities = 78/78 (100%)
 Strand = Plus / Minus


Query: 417    tataaagaagaattaaataatgttacaaatttaataatacaagaatttgaacaatctaaa 476
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526986 tataaagaagaattaaataatgttacaaatttaataatacaagaatttgaacaatctaaa 526927


Query: 477    atttcatcattagcaacc 494
              ||||||||||||||||||
Sbjct: 526926 atttcatcattagcaacc 526909

 Score =  141 bits (71), Expect(9) = 0.0
 Identities = 71/71 (100%)
 Strand = Plus / Minus


Query: 1040   gtctaaaggtagaaataaagatttatatttgtataataatacttttacaaacattttaca 1099
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526266 gtctaaaggtagaaataaagatttatatttgtataataatacttttacaaacattttaca 526207


Query: 1100   attaaaaaatg 1110
              |||||||||||
Sbjct: 526206 attaaaaaatg 526196

 Score =  111 bits (56), Expect(9) = 0.0
 Identities = 56/56 (100%)
 Strand = Plus / Minus


Query: 148    tgacaaataccaataactacgatgaagaaaaagatgaagatggagaaaatgatgac 203
              ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 527255 tgacaaataccaataactacgatgaagaaaaagatgaagatggagaaaatgatgac 527200

 Score = 73.8 bits (37), Expect(9) = 0.0
 Identities = 37/37 (100%)
 Strand = Plus / Minus


Query: 1110   gatttagtatcaatgatccaaagtccaatgttcaaag 1146
              |||||||||||||||||||||||||||||||||||||
Sbjct: 526114 gatttagtatcaatgatccaaagtccaatgttcaaag 526078

 Score = 52.0 bits (26), Expect(9) = 0.0
 Identities = 26/26 (100%)
 Strand = Plus / Minus


Query: 735    gttccgacattacaaccactatcttt 760
              ||||||||||||||||||||||||||
Sbjct: 526668 gttccgacattacaaccactatcttt 526643

 Score = 34.2 bits (17), Expect(17) = 0.57
 Identities = 23/25 (92%)
 Strand = Plus / Minus


Query: 291    acaactccagcttcaacaacaccaa 315
              ||||| ||| |||||||||||||||
Sbjct: 527097 acaacaccaacttcaacaacaccaa 527073

 Score = 32.2 bits (16), Expect(17) = 0.57
 Identities = 16/16 (100%)
 Strand = Plus / Minus


Query: 1099  aattaaaaaatgattt 1114
             ||||||||||||||||
Sbjct: 17035 aattaaaaaatgattt 17020

 Score = 30.2 bits (15), Expect(17) = 0.57
 Identities = 15/15 (100%)
 Strand = Plus / Minus


Query: 1065  tatttgtataataat 1079
             |||||||||||||||
Sbjct: 44205 tatttgtataataat 44191

 Score = 30.2 bits (15), Expect(17) = 0.57
 Identities = 18/19 (94%)
 Strand = Plus / Minus


Query: 1047   ggtagaaataaagatttat 1065
              ||||||||||||| |||||
Sbjct: 191669 ggtagaaataaagttttat 191651

 Score = 30.2 bits (15), Expect(17) = 0.57
 Identities = 15/15 (100%)
 Strand = Plus / Minus


Query: 427    aattaaataatgtta 441
              |||||||||||||||
Sbjct: 513865 aattaaataatgtta 513851

 Score = 28.2 bits (14), Expect(17) = 0.57
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 908    tgaatttggtgaat 921
              ||||||||||||||
Sbjct: 215994 tgaatttggtgaat 215981

 Score = 28.2 bits (14), Expect(17) = 0.57
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 582    aataaaatatttga 595
              ||||||||||||||
Sbjct: 322398 aataaaatatttga 322385

 Score = 28.2 bits (14), Expect(17) = 0.57
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 571    ttcaattaattaat 584
              ||||||||||||||
Sbjct: 438860 ttcaattaattaat 438847

 Score = 28.2 bits (14), Expect(17) = 0.57
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 479    ttcatcattagcaa 492
              ||||||||||||||
Sbjct: 489958 ttcatcattagcaa 489945

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 1091  cattttacaatta 1103
             |||||||||||||
Sbjct: 18052 cattttacaatta 18040

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 984    aaaataccttcaa 996
              |||||||||||||
Sbjct: 192520 aaaataccttcaa 192508

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 895    cacaaagtttaaa 907
              |||||||||||||
Sbjct: 217504 cacaaagtttaaa 217492

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 622    caaataatttagt 634
              |||||||||||||
Sbjct: 281193 caaataatttagt 281181

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 610    ttgatggtaaatc 622
              |||||||||||||
Sbjct: 300860 ttgatggtaaatc 300848

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 470    atctaaaatttca 482
              |||||||||||||
Sbjct: 500867 atctaaaatttca 500855

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 314    aacttcaacaaca 326
              |||||||||||||
Sbjct: 518205 aacttcaacaaca 518193

 Score = 26.3 bits (13), Expect(17) = 0.57
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 151    caaataccaataa 163
              |||||||||||||
Sbjct: 531488 caaataccaataa 531476

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,039,236,315
Number of extensions: 69695361
Number of successful extensions: 5944183
Number of sequences better than 10.0: 146
Length of query: 1353
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1329
Effective length of database: 97,308,875,965
Effective search space: 129323496157485
Effective search space used: 129323496157485
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10335-1 (Contig-U10335-1Q) /CSM_Contig/Contig-U10335-1Q.Seq.d
         (1353 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_199(AC116984|pid:none) Dictyostelium discoideum chromos...   348   3e-94

>AC116984_199(AC116984|pid:none) Dictyostelium discoideum chromosome 2
            map 2567470-3108875 strain AX4, complete sequence.
          Length = 366

 Score =  348 bits (893), Expect = 3e-94
 Identities = 208/364 (57%), Positives = 209/364 (57%)
 Frame = +3

Query: 216  IINGLIFDIDKLEKEIIELQEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 395
            IINGLIFDIDKLEKEIIELQE
Sbjct: 24   IINGLIFDIDKLEKEIIELQETKTSTTPASTTPTSTTPRSKSSSDLQQSESHQQQQQQPQ 83

Query: 396  XXXXTPNYKEELNNVTNLIIQEFEQSKISSLATXXXXXXXKRITIPDNHSNNPDKLLEIQ 575
                TPNYKEELNNVTNLIIQEFEQSKISSLAT       KRITIPDNHSNNPDKLLEIQ
Sbjct: 84   NKSQTPNYKEELNNVTNLIIQEFEQSKISSLATNNNINNNKRITIPDNHSNNPDKLLEIQ 143

Query: 576  LINKIFDISKAFDGKSNNLVSSFQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPTLQPL 755
            LINKIFDISKAFDGKSNNLVSSFQ                             VPTLQPL
Sbjct: 144  LINKIFDISKAFDGKSNNLVSSFQNCTNNNNNNNNNTDNNNNNNISNNNNNNNVPTLQPL 203

Query: 756  SFNNRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTIGSLRDLNKIQPQSLNEFGEWKSLI 935
            SFNNR                             TIGSLRDLNKIQPQSLNEFGEWKSLI
Sbjct: 204  SFNNRNNLVNGNISSSSSSNSSNNNIGSSNSNNVTIGSLRDLNKIQPQSLNEFGEWKSLI 263

Query: 936  DEELSDLYTLVKELKRKIPXXXXXXXXXXITDSPVSKGRNKDLYLYNNTFTNILQLKNDL 1115
            DEELSDLYTLVKELKRKIP          ITDSPV                       DL
Sbjct: 264  DEELSDLYTLVKELKRKIPSNTRTTSSNNITDSPV-----------------------DL 300

Query: 1116 VSMIQSPMFKDNEYNKHNIGSDSYXXXXXXXXXXXXXKDDGTSTNTAGGGSPNKKSVKQL 1295
            VSMIQSPMFKDNEYNKHNIGSDSY             KDDGTSTNTAGGGSPNKKSVKQL
Sbjct: 301  VSMIQSPMFKDNEYNKHNIGSDSYSSNNNSNNNLSNLKDDGTSTNTAGGGSPNKKSVKQL 360

Query: 1296 VYIF 1307
            V +F
Sbjct: 361  VLLF 364

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,010,497,516
Number of extensions: 11529976
Number of successful extensions: 24958
Number of sequences better than 10.0: 1
Number of HSP's gapped: 24945
Number of HSP's successfully gapped: 2
Length of query: 451
Length of database: 1,040,966,779
Length adjustment: 132
Effective length of query: 319
Effective length of database: 618,001,159
Effective search space: 197142369721
Effective search space used: 197142369721
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.56  gvh: 0.32  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial

>> prediction for Contig-U10335-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 2
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0