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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10327-1
Contig ID Contig-U10327-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10327-1 (Contig-U10327-1Q) /CSM_Contig/Contig-U10327-1Q.Seq.d
AATTTTGTAAAACCACCCGAAAGTAACAGTAATATTAAGATGGAAGTAGG
TATCGAAGATTGTCTTCACATTGAATTCGAATACAACAAATCAAAATACC
ATTTGAAGGATGTTATCATTGGTAAAATTTATTTCCTTTTAGTTAGAATT
AAAATCAAATATATGGAAATTGCACTCATTAAAAGAGAATCAACTGGTTC
AGGTCCAAACGTTTTTAATGAAAGTGAAACTTTAACTAAATTTGAAATTA
TGGATGGTGCACCCAGTTAGAGGTGAATCTATTCCTGTTAGACTTTTCTT
ATCAGTATTTGATCTCACACCAACCTATAGAAGTGTACATAATAAGTTTT
CTGTTAAATATTTCCTCAATCTTGCACTTGTTGATGAAGATGATAAAAAA
TACTTTAAACAACAAGAAATTACACTTTGGAGAAGAGGTGTAACTAAACC
AGGCTTACCTGGCTCAACTAACAACAACAATAACAACAATACTTCAAACA
CCAACAATACCAATACACCACAACAAAAAGTAACCGAATCTCAAAATGAA
GTACCAGAACCAGAAAATAACTCTTCCGATGAAAGTGATTAAAATTATAA
ATAATAATAATAATAAAAATAATAATAATAATAATAATAAATAAAATCTA
AAATAATATCATTATACTACACTTACATTCGCCAATCGTTTTCTCTTCCT
CTTCTCTCTCATTATCCTAATAAATAAAAAAAATAAAATAAAAAAAATTA
AAATAATAATAATAATTAGAAATAAAAAATAAATAAAGTAAATGTTTTTT
TTTTTTTTATAAATAAAA
Gap no gap
Contig length 818
Chromosome number (1..6, M) 1
Chromosome length 4919822
Start point 4315425
End point 4314607
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 3
Link to clone list U10327
List of clone(s) 

est1=VFM149Z,1,699
est2=SSH595F,117,427
est3=VSA752E,252,819
Translated Amino Acid sequence 
il*nhpkvtvilrwk*vskivftlnsnttnqnti*rmlslvkfisf*lelksniwklhsl
kenqlvqvqtflmkvkl*LNLKLWMVHPVRGESIPVRLFLSVFDLTPTYRSVHNKFSVKY
FLNLALVDEDDKKYFKQQEITLWRRGVTKPGLPGSTNNNNNNNTSNTNNTNTPQQKVTES
QNEVPEPENNSSDESD*nyk*****k*******ikskiislyytyirqsfslpllshypn
k*kk*nkkn*nnnnn*k*kinkvnvffffink
Translated Amino Acid sequence (All Frames) 
Frame A:
nfvkppesnsnikmevgiedclhiefeynkskyhlkdviigkiyfllvrikikymeiali
krestgsgpnvfnesetltkfeimdgaps*r*iysc*tflisi*shtnl*kct**vfc*i
fpqsctc**r**kil*ttrnytlekrcn*trltwln*qqq*qqyfkhqqyqytttksnri
sk*strtrk*lfr*k*lkl*iiiiikiiiiiiinki*nniiilhlhspivfsssslsls*
*ikkik*kklk****leiknk*skcffffyk*


Frame B:
il*nhpkvtvilrwk*vskivftlnsnttnqnti*rmlslvkfisf*lelksniwklhsl
kenqlvqvqtflmkvkl*LNLKLWMVHPVRGESIPVRLFLSVFDLTPTYRSVHNKFSVKY
FLNLALVDEDDKKYFKQQEITLWRRGVTKPGLPGSTNNNNNNNTSNTNNTNTPQQKVTES
QNEVPEPENNSSDESD*nyk*****k*******ikskiislyytyirqsfslpllshypn
k*kk*nkkn*nnnnn*k*kinkvnvffffink


Frame C:
fckttrk*q*y*dgsryrrlssh*iriqqikipfegcyhw*nlfpfs*n*nqiygncth*
krinwfrskrf**k*nfn*i*nygwctqlevnlflldfsyqylishqpievyiisfllni
ssilhllmkmikntlnnkklhfgeev*lnqaylaqltttittilqtptipihhnkk*pnl
kmkyqnqkitlpmkvikiinnnnnknnnnnnnk*nlk*yhyttltfanrflflfsliili
nkknkikkikiiiiirnkk*ik*mffffl*ik
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10327-1 (Contig-U10327-1Q)
/CSM_Contig/Contig-U10327-1Q.Seq.d
         (818 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10327-1 (Contig-U10327-1Q) /CSM_Contig/Conti...   920  0.0
Contig-U09417-1 (Contig-U09417-1Q) /CSM_Contig/Conti...    38  0.015
Contig-U09368-1 (Contig-U09368-1Q) /CSM_Contig/Conti...    38  0.015
Contig-U14020-1 (Contig-U14020-1Q) /CSM_Contig/Conti...    36  0.058
Contig-U04570-1 (Contig-U04570-1Q) /CSM_Contig/Conti...    36  0.058
Contig-U13418-1 (Contig-U13418-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U11899-1 (Contig-U11899-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U11227-1 (Contig-U11227-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U11000-1 (Contig-U11000-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U08357-1 (Contig-U08357-1Q) /CSM_Contig/Conti...    34  0.23

>Contig-U10327-1 (Contig-U10327-1Q) /CSM_Contig/Contig-U10327-1Q.Seq.d
          Length = 818

 Score =  920 bits (464), Expect = 0.0
 Identities = 464/464 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   aattttgtaaaaccacccgaaagtaacagtaatattaagatggaagtaggtatcgaagat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aattttgtaaaaccacccgaaagtaacagtaatattaagatggaagtaggtatcgaagat 60

                                                                       
Query: 61  tgtcttcacattgaattcgaatacaacaaatcaaaataccatttgaaggatgttatcatt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tgtcttcacattgaattcgaatacaacaaatcaaaataccatttgaaggatgttatcatt 120

                                                                       
Query: 121 ggtaaaatttatttccttttagttagaattaaaatcaaatatatggaaattgcactcatt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 ggtaaaatttatttccttttagttagaattaaaatcaaatatatggaaattgcactcatt 180

                                                                       
Query: 181 aaaagagaatcaactggttcaggtccaaacgtttttaatgaaagtgaaactttaactaaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 aaaagagaatcaactggttcaggtccaaacgtttttaatgaaagtgaaactttaactaaa 240

                                                                       
Query: 241 tttgaaattatggatggtgcacccagttagaggtgaatctattcctgttagacttttctt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tttgaaattatggatggtgcacccagttagaggtgaatctattcctgttagacttttctt 300

                                                                       
Query: 301 atcagtatttgatctcacaccaacctatagaagtgtacataataagttttctgttaaata 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 atcagtatttgatctcacaccaacctatagaagtgtacataataagttttctgttaaata 360

                                                                       
Query: 361 tttcctcaatcttgcacttgttgatgaagatgataaaaaatactttaaacaacaagaaat 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 tttcctcaatcttgcacttgttgatgaagatgataaaaaatactttaaacaacaagaaat 420

                                                       
Query: 421 tacactttggagaagaggtgtaactaaaccaggcttacctggct 464
           ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 tacactttggagaagaggtgtaactaaaccaggcttacctggct 464


 Score =  176 bits (89), Expect = 2e-44
 Identities = 89/89 (100%)
 Strand = Plus / Plus

                                                                       
Query: 508 taccaatacaccacaacaaaaagtaaccgaatctcaaaatgaagtaccagaaccagaaaa 567
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 508 taccaatacaccacaacaaaaagtaaccgaatctcaaaatgaagtaccagaaccagaaaa 567

                                        
Query: 568 taactcttccgatgaaagtgattaaaatt 596
           |||||||||||||||||||||||||||||
Sbjct: 568 taactcttccgatgaaagtgattaaaatt 596


 Score =  117 bits (59), Expect = 2e-26
 Identities = 59/59 (100%)
 Strand = Plus / Plus

                                                                      
Query: 659 tcattatactacacttacattcgccaatcgttttctcttcctcttctctctcattatcc 717
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 659 tcattatactacacttacattcgccaatcgttttctcttcctcttctctctcattatcc 717


>Contig-U09417-1 (Contig-U09417-1Q) /CSM_Contig/Contig-U09417-1Q.Seq.d
          Length = 621

 Score = 38.2 bits (19), Expect = 0.015
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 341 aataagttttctgttaaatattt 363
           ||||| |||||||||||||||||
Sbjct: 437 aataaattttctgttaaatattt 459


>Contig-U09368-1 (Contig-U09368-1Q) /CSM_Contig/Contig-U09368-1Q.Seq.d
          Length = 851

 Score = 38.2 bits (19), Expect = 0.015
 Identities = 25/27 (92%)
 Strand = Plus / Plus

                                      
Query: 202 ggtccaaacgtttttaatgaaagtgaa 228
           |||| |||| |||||||||||||||||
Sbjct: 54  ggtcaaaacttttttaatgaaagtgaa 80


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8306
Number of Sequences: 6905
Number of extensions: 8306
Number of successful extensions: 985
Number of sequences better than 10.0: 113
length of query: 818
length of database: 5,674,871
effective HSP length: 16
effective length of query: 802
effective length of database: 5,564,391
effective search space: 4462641582
effective search space used: 4462641582
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.19
Homology vs DNA 
Query= Contig-U10327-1 (Contig-U10327-1Q) /CSM_Contig/Contig-U10327-1Q.Seq.d
         (818 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ434933) Dictyostelium discoideum cDNA clone:ddv25a13, 3' ...   521   0.0    4
(U61985) Dictyostelium discoideum PepA (pepA) mRNA, complete...   505   0.0    4
(AU261710) Dictyostelium discoideum vegetative cDNA clone:VS...   422   e-171  3
(AU073583) Dictyostelium discoideum slug cDNA, clone SSH595.      291   e-125  2
(CO765899) spleen_EST01625 Spleen cDNA Library Gallus gallus...   100   5e-25  2
(FE035674) C0009483N24.P1KM13F KN511 Ovis aries peyer's patc...   101   2e-22  3
(FE035675) C0009483N24.Q1KM13R KN511 Ovis aries peyer's patc...   101   3e-22  3
(FE027701) C0009263A09.Q1KAM13R KN511 Ovis aries abomasal mu...   101   4e-22  3
(BE238897) MD0553 Meloidogyne incognita J2 (#MD99-1) Meloido...    92   9e-22  3
(DY885448) CeleSEQ695 Cunninghamella elegans pBluescript (Ec...    76   8e-21  3
(DY892651) CeleSEQ11240 Cunninghamella elegans pBluescript (...    76   9e-21  3
(FE027700) C0009263A09.P1KAM13F KN511 Ovis aries abomasal mu...   101   2e-20  2
(EE834327) 021030OOCX031021HT OOCX Ovis aries cDNA, mRNA seq...   101   2e-20  2
(CO773357) testis_EST04454 Testis cDNA Library Gallus gallus...   100   1e-19  2
(CO505776) GGEZEB1007G07.g embryo breast muscle - EB1 Gallus...   100   2e-19  2
(BU443039) 604142376F1 CSEQRBN11 Gallus gallus cDNA clone Ch...   100   3e-19  2
(AJ958138) Sus scrofa EST, clone C0007756n20, 5' direction.        94   3e-19  2
(BU143956) 603228425F1 CSEQCHL26 Gallus gallus cDNA clone Ch...   100   3e-19  2
(AJ955403) Sus scrofa EST, clone C0007744n15, 5' direction.        94   3e-19  2
(AJ954772) Sus scrofa EST, clone C0007744n15, 3' direction.        94   3e-19  2
(CF253761) mdvn107_f12 Marek's disease virus-infected spleen...   100   4e-19  2
(BU111838) 603128172F1 CSEQCHL13 Gallus gallus cDNA clone Ch...   100   4e-19  2
(DR417880) gPGC_EST07722 Embryonic gonadal PGC cDNA Library ...   100   6e-19  2
(EE834731) 020527OOCX003033HT OOCX Ovis aries cDNA, mRNA seq...   101   8e-19  2
(AK231106) Sus scrofa mRNA, clone:AMP010094C02, expressed in...    94   9e-19  2
(CB532756) 757235 MARC 6BOV Bos taurus cDNA 5', mRNA sequence.    105   3e-18  1
(BQ750303) SnEST4a70h12.y1 cSn 1 3 S neurona subtracted cDNA...   100   1e-17  2
(CK966542) 4081910 BARC 9BOV Bos taurus cDNA clone 9BOV1_N15...   100   1e-17  2
(CR551296) Normalized and Subtracted bovine embryonic and ex...   100   2e-17  2
(DY082023) 001018BUTA001144HT BUTA Bos taurus cDNA 5', mRNA ...   100   2e-17  2
(CB533105) 757683 MARC 6BOV Bos taurus cDNA 3', mRNA sequence.    100   2e-17  2
(DN279540) 1160581 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.   100   2e-17  2
(BJ928029) Daphnia magna cDNA, clone:IGU001_0028_G04.f, 5' e...    72   2e-17  2
(EE858741) 010914OSTA118047HT OSTA Ovis aries cDNA, mRNA seq...   101   4e-17  1
(EY263519) BF01025B2G06.f1 Normalized subtracted keck librar...   101   4e-17  1
(CV037253) 4140295 BARC_3GAL chicken mixed tissue Gallus gal...    92   5e-17  2
(BT030523) Bos taurus vacuolar protein sorting 26 homolog A ...   100   6e-17  2
(BC119831) Bos taurus vacuolar protein sorting 26 homolog A ...   100   6e-17  2
(CN163118) 952586 MARC 4PIG Sus scrofa cDNA 3', mRNA sequence.     94   7e-17  2
(CN161732) 951050 MARC 4PIG Sus scrofa cDNA 5', mRNA sequence.     94   7e-17  2
(BU135298) 603122349F1 CSEQCHL23 Gallus gallus cDNA clone Ch...    92   7e-17  2
(EX828606) KMR3635_B06_004_12-15-05-3130-1 Meleagris gallopa...    92   8e-17  2
(CV291184) aof01-15ms2-b12 Aof01 Asparagus officinalis cDNA ...   100   8e-17  2
(ES346146) u3c23ca.r1 cattle subcutaneous adipose tissue cDN...   100   2e-16  1
(EL600564) He_pwd_0198B07_M13R Heliconius erato pooled wing ...   100   2e-16  1
(EH171493) LB01643.CR_A02 GC_BGC-16 Bos taurus cDNA clone IM...   100   2e-16  1
(EE371450) LB03030.CR_O05 GC_BGC-30 Bos taurus cDNA clone IM...   100   2e-16  1
(EE334292) LB01736.CR_C02 GC_BGC-17 Bos taurus cDNA clone IM...   100   2e-16  1
(DV817084) LB01721.CR_L10 GC_BGC-17 Bos taurus cDNA clone IM...   100   2e-16  1
(DT890858) 1471926 MARC 7BOV Bos taurus cDNA 5', mRNA sequence.   100   2e-16  1
(DN281321) 1162501 MARC 7BOV Bos taurus cDNA 5', mRNA sequence.   100   2e-16  1
(AJ455688) Gallus gallus EST, clone library riken1, clone 6d...   100   2e-16  1
(AJ453234) Gallus gallus EST, clone library riken1, clone 33...   100   2e-16  1
(BU467714) 603373029F1 CSEQRBN20 Gallus gallus cDNA clone Ch...    72   2e-16  3
(DN441311) LIB5338-102-A1-K2-E5 LIB5338 Canis lupus familiar...    86   2e-16  2
(BU107260) 603111117F1 CSEQCHL12 Gallus gallus cDNA clone Ch...    72   3e-16  3
(CX984520) DOG_EST_P05G2 Canis familiaris testis cDNA Canis ...    86   3e-16  2
(CO103893) GR__Eb0032L09.r GR__Eb Gossypium raimondii cDNA c...    58   6e-16  3
(AM910996) Plasmodium knowlesi strain H chromosome 14, compl...    84   1e-15  2
(DN315284) PL06020B1E05 cDNA from juvenile hermaphodites Sch...    96   2e-15  1
(CB034035) EFCP040A03 flower bud Lambda ZapII cDNA Library P...    80   3e-15  3
(CK856148) EFCPL1-01F2 Flower bud of Phalaenopsis equestris ...    80   4e-15  3
(DN119494) 1119426 MARC 4PIG Sus scrofa cDNA 5', mRNA sequence.    94   4e-15  2
(AJ957336) Sus scrofa EST, clone C0007756n20, 3' direction.        86   4e-15  2
(EH172458) LB01645.CR.1_P04 GC_BGC-16 Bos taurus cDNA clone ...    94   1e-14  1
(DB798255) Sus scrofa mRNA, clone:DCI010098D01, 5' end, expr...    94   1e-14  1
(CV871917) PDUts1096G12 Porcine testis cDNA library I Sus sc...    94   1e-14  1
(EW635287) rtes37_d20r1.y1 tes Sus scrofa cDNA 5', mRNA sequ...    94   1e-14  1
(EW296793) ruio05_d24.y1 kid Sus scrofa cDNA 5', mRNA sequence.    94   1e-14  1
(EW032233) rcte14_l6.y1 cte Sus scrofa cDNA 5', mRNA sequence.     94   1e-14  1
(BC046056) Danio rerio vacuolar protein sorting 26 homolog B...    92   4e-14  1
(EH593180) FDR306-P00040-DEPE-F_N21 FDR306 Danio rerio cDNA ...    92   4e-14  1
(EG582344) AGENCOURT_88163030 NIH_ZGC_35 Danio rerio cDNA cl...    92   4e-14  1
(EE691942) AGENCOURT_90033939 NIH_ZGC_28 Danio rerio cDNA cl...    92   4e-14  1
(EB983687) 15506412 ZF40 Danio rerio cDNA clone 4224664, mRN...    92   4e-14  1
(DB919512) Idiosepius paradoxus cDNA, clone:Ip_aB_035_G04, 5...    92   4e-14  1
(CK237700) AGENCOURT_16972903 NCI_CGAP_ZKid1 Danio rerio cDN...    92   4e-14  1
(AJ445551) Gallus gallus EST, clone library riken1, clone 10...    92   4e-14  1
(CD760293) AGENCOURT_14627415 NCI_CGAP_ZKid1 Danio rerio cDN...    92   4e-14  1
(CD758456) AGENCOURT_14621717 NCI_CGAP_ZKid1 Danio rerio cDN...    92   4e-14  1
(CD758444) AGENCOURT_14621719 NCI_CGAP_ZKid1 Danio rerio cDN...    92   4e-14  1
(CD284020) G39172.41 NCI_CGAP_ZKid1 Danio rerio cDNA clone I...    92   4e-14  1
(CB353380) ZF001-P00006-DPE-F2-D_H06 GISZF001 Danio rerio cD...    92   4e-14  1
(CA472320) AGENCOURT_10739409 NCI_CGAP_ZKid1 Danio rerio cDN...    92   4e-14  1
(BW961925) Sus scrofa mRNA, clone:AMP010094C02, 5' end, expr...    92   4e-14  1
(EE008439) ROE00003596 Rhizopus oryzae Company Rhizopus oryz...    80   6e-14  2
(CX988467) DOG_EST_P55B09 Canis familiaris testis cDNA Canis...    78   6e-14  2
(FG710994) G1144P324FC5.T0 Anolis carolinensis pooled normal...    86   6e-14  2
(AM095664) Lutzomyia longipalpis EST clone NSFM-138g06, sequ...    90   1e-13  2
(AA282859) zs91a05.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone ...    78   1e-13  2
(ES833112) UFL_323_47 Cotton fiber 0-10 day post anthesis Go...    50   1e-13  3
(ES799843) UFL_539_30 Cotton fiber 0-10 day post anthesis Go...    50   1e-13  3
(DA792277) Homo sapiens cDNA clone OCBBF2031673, 5' end, mRN...    78   2e-13  2
(DB211649) Homo sapiens cDNA clone TRACH2025135, 5' end, mRN...    78   2e-13  2
(DA938078) Homo sapiens cDNA clone SPLEN2011147, 5' end, mRN...    78   2e-13  2
(DA602697) Homo sapiens cDNA clone HSYRA2008454, 5' end, mRN...    78   2e-13  2
(DA509954) Homo sapiens cDNA clone FCBBF3026623, 5' end, mRN...    78   2e-13  2
(DA839771) Homo sapiens cDNA clone PLACE6006408, 5' end, mRN...    78   2e-13  2
(DA998231) Homo sapiens cDNA clone T1ESE2002402, 5' end, mRN...    78   2e-13  2
(BP244052) Homo sapiens cDNA clone: HEP22687, Sugano cDNA li...    78   2e-13  2

>(BJ434933) Dictyostelium discoideum cDNA clone:ddv25a13, 3' end,
           single read.
          Length = 698

 Score =  521 bits (263), Expect(4) = 0.0
 Identities = 263/263 (100%)
 Strand = Plus / Minus


Query: 1   aattttgtaaaaccacccgaaagtaacagtaatattaagatggaagtaggtatcgaagat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 698 aattttgtaaaaccacccgaaagtaacagtaatattaagatggaagtaggtatcgaagat 639


Query: 61  tgtcttcacattgaattcgaatacaacaaatcaaaataccatttgaaggatgttatcatt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 638 tgtcttcacattgaattcgaatacaacaaatcaaaataccatttgaaggatgttatcatt 579


Query: 121 ggtaaaatttatttccttttagttagaattaaaatcaaatatatggaaattgcactcatt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 578 ggtaaaatttatttccttttagttagaattaaaatcaaatatatggaaattgcactcatt 519


Query: 181 aaaagagaatcaactggttcaggtccaaacgtttttaatgaaagtgaaactttaactaaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 518 aaaagagaatcaactggttcaggtccaaacgtttttaatgaaagtgaaactttaactaaa 459


Query: 241 tttgaaattatggatggtgcacc 263
           |||||||||||||||||||||||
Sbjct: 458 tttgaaattatggatggtgcacc 436

 Score =  400 bits (202), Expect(4) = 0.0
 Identities = 202/202 (100%)
 Strand = Plus / Minus


Query: 263 ccagttagaggtgaatctattcctgttagacttttcttatcagtatttgatctcacacca 322
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 437 ccagttagaggtgaatctattcctgttagacttttcttatcagtatttgatctcacacca 378


Query: 323 acctatagaagtgtacataataagttttctgttaaatatttcctcaatcttgcacttgtt 382
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 377 acctatagaagtgtacataataagttttctgttaaatatttcctcaatcttgcacttgtt 318


Query: 383 gatgaagatgataaaaaatactttaaacaacaagaaattacactttggagaagaggtgta 442
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 317 gatgaagatgataaaaaatactttaaacaacaagaaattacactttggagaagaggtgta 258


Query: 443 actaaaccaggcttacctggct 464
           ||||||||||||||||||||||
Sbjct: 257 actaaaccaggcttacctggct 236

 Score =  125 bits (63), Expect(4) = 0.0
 Identities = 63/63 (100%)
 Strand = Plus / Minus


Query: 527 aaagtaaccgaatctcaaaatgaagtaccagaaccagaaaataactcttccgatgaaagt 586
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 173 aaagtaaccgaatctcaaaatgaagtaccagaaccagaaaataactcttccgatgaaagt 114


Query: 587 gat 589
           |||
Sbjct: 113 gat 111

 Score = 81.8 bits (41), Expect(4) = 0.0
 Identities = 41/41 (100%)
 Strand = Plus / Minus


Query: 659 tcattatactacacttacattcgccaatcgttttctcttcc 699
           |||||||||||||||||||||||||||||||||||||||||
Sbjct: 41  tcattatactacacttacattcgccaatcgttttctcttcc 1

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 744,761,378
Number of extensions: 46178736
Number of successful extensions: 4074534
Number of sequences better than 10.0: 999
Length of query: 818
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 794
Effective length of database: 97,308,875,965
Effective search space: 77263247516210
Effective search space used: 77263247516210
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10327-1 (Contig-U10327-1Q) /CSM_Contig/Contig-U10327-1Q.Seq.d
         (818 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U61985_1(U61985|pid:none) Dictyostelium discoideum PepA (pepA) m...   181   9e-91
(Q6DH23) RecName: Full=Vacuolar protein sorting-associated prote...   147   5e-56
(Q6DFB9) RecName: Full=Vacuolar protein sorting-associated prote...   147   6e-56
BC098386_1(BC098386|pid:none) Homo sapiens vacuolar protein sort...   147   8e-56
FJ217219_1(FJ217219|pid:none) Sus scrofa vacuolar protein sortin...   147   8e-56
BC029758_1(BC029758|pid:none) Mus musculus vacuolar protein sort...   147   8e-56
EF087923_1(EF087923|pid:none) Picea sitchensis clone WS02743_E16...   140   1e-55
(Q5R436) RecName: Full=Vacuolar protein sorting-associated prote...   146   2e-55
(Q7ZV03) RecName: Full=Vacuolar protein sorting-associated prote...   144   2e-55
AE017344_532(AE017344|pid:none) Cryptococcus neoformans var. neo...   151   4e-55
DQ200367_1(DQ200367|pid:none) Solanum tuberosum clone 056D03 vac...   140   5e-55
AL021106_6(AL021106|pid:none) Drosophila melanogaster cosmid clo...   146   6e-55
(Q9W552) RecName: Full=Vacuolar protein sorting-associated prote...   146   6e-55
Z68220_9(Z68220|pid:none) Caenorhabditis elegans Cosmid T20D3, c...   143   6e-55
BT040254_1(BT040254|pid:none) Zea mays full-length cDNA clone ZM...   135   2e-53
BT035714_1(BT035714|pid:none) Zea mays full-length cDNA clone ZM...   139   2e-53
EU975813_1(EU975813|pid:none) Zea mays clone 501783 vacuolar pro...   139   3e-53
(Q6TNP8) RecName: Full=Vacuolar protein sorting-associated prote...   136   3e-53
AB015476_6(AB015476|pid:none) Arabidopsis thaliana genomic DNA, ...   136   3e-53
(Q9T091) RecName: Full=Vacuolar protein sorting-associated prote...   133   6e-53
BC049342_1(BC049342|pid:none) Danio rerio vacuolar protein sorti...   134   1e-52
(Q6AY86) RecName: Full=Vacuolar protein sorting-associated prote...   137   1e-52
AK073408_1(AK073408|pid:none) Oryza sativa Japonica Group cDNA c...   134   1e-52
AY803784_1(AY803784|pid:none) Hartmannella vermiformis vacuolar ...   133   1e-52
AK168213_1(AK168213|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...   136   3e-52
(P40336) RecName: Full=Vacuolar protein sorting-associated prote...   136   3e-52
(O75436) RecName: Full=Vacuolar protein sorting-associated prote...   136   3e-52
(Q0VD53) RecName: Full=Vacuolar protein sorting-associated prote...   136   3e-52
(Q28HT6) RecName: Full=Vacuolar protein sorting-associated prote...   135   4e-52
(Q6IRD0) RecName: Full=Vacuolar protein sorting-associated prote...   135   5e-52
AK168537_1(AK168537|pid:none) Mus musculus 17 days pregnant adul...   135   6e-52
(Q6DFU4) RecName: Full=Vacuolar protein sorting-associated prote...   135   6e-52
AM920437_520(AM920437|pid:none) Penicillium chrysogenum Wisconsi...   145   8e-52
AP007155_870(AP007155|pid:none) Aspergillus oryzae RIB40 genomic...   145   8e-52
AM910996_672(AM910996|pid:none) Plasmodium knowlesi strain H chr...   134   2e-51
AK297142_1(AK297142|pid:none) Homo sapiens cDNA FLJ59716 complet...   133   2e-51
T25029(T25029)hypothetical protein T20D3.7 - Caenorhabditis eleg...   143   9e-51
(Q8I4T1) RecName: Full=Vacuolar protein sorting-associated prote...   133   1e-50
CP000584_63(CP000584|pid:none) Ostreococcus lucimarinus CCE9901 ...   124   4e-50
AF114171_13(AF114171|pid:none) Sorghum bicolor BAC clone 25.M18,...   140   1e-48
CR382130_955(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   135   4e-48
(Q10243) RecName: Full=Vacuolar protein sorting-associated prote...   128   4e-48
CP000502_109(CP000502|pid:none) Pichia stipitis CBS 6054 chromos...   123   9e-45
CR940353_715(CR940353|pid:none) Theileria annulata strain Ankara...   117   4e-44
CP001159_288(CP001159|pid:none) Thalassiosira pseudonana CCMP133...   116   9e-44
EU974982_1(EU974982|pid:none) Zea mays clone 462190 unknown mRNA.     139   2e-37
AE016818_169(AE016818|pid:none) Ashbya gossypii (= Eremothecium ...   107   2e-36
AY193839_1(AY193839|pid:none) Entamoeba histolytica vacuolar sor...    90   1e-34
AK165539_1(AK165539|pid:none) Mus musculus brain CRL-1443 BC3H1 ...   147   3e-34
AY811581_1(AY811581|pid:none) Schistosoma japonicum SJCHGC05566 ...   128   3e-28
CT005272_353(CT005272|pid:none) Leishmania major strain Friedlin...    71   3e-26
AM502254_336(AM502254|pid:none) Leishmania infantum chromosome 36.     71   5e-26
AY193840_1(AY193840|pid:none) Giardia intestinalis vacuolar prot...    44   7e-10
AY193846_1(AY193846|pid:none) Spironucleus barkhanus vacuolar pr...    42   0.018
AE016877_2174(AE016877|pid:none) Bacillus cereus ATCC 14579, com...    33   8.5

>U61985_1(U61985|pid:none) Dictyostelium discoideum PepA (pepA)
           mRNA, complete cds.
          Length = 349

 Score =  181 bits (460), Expect(2) = 9e-91
 Identities = 91/109 (83%), Positives = 91/109 (83%)
 Frame = +2

Query: 263 PVRGESIPVRLFLSVFDLTPTYRSVHNKFSVKYFLNLALVDEDDKKYFKQQEITLWRRGV 442
           PVRGESIPVRLFLSVFDLTPTYRSVHNKFSVKYFLNLALVDEDDKKYFKQQEITLWRRGV
Sbjct: 241 PVRGESIPVRLFLSVFDLTPTYRSVHNKFSVKYFLNLALVDEDDKKYFKQQEITLWRRGV 300

Query: 443 TKPGLPGXXXXXXXXXXXXXXXXXXPQQKVTESQNEVPEPENNSSDESD 589
           TKPGLPG                  PQQKVTESQNEVPEPENNSSDESD
Sbjct: 301 TKPGLPGSTNNNNNNNTSNTNNTNTPQQKVTESQNEVPEPENNSSDESD 349

 Score =  176 bits (446), Expect(2) = 9e-91
 Identities = 86/88 (97%), Positives = 86/88 (97%)
 Frame = +1

Query: 1   NFVKPPESNSNIKMEVGIEDCLHIEFEYNKSKYHLKDVIIGKIYFLLVRIKIKYMEIALI 180
           NFVKPPESNSNIKMEVGIEDCLHIEFEYNKSKYHLKDVIIG  YFLLVRIKIKYMEIALI
Sbjct: 154 NFVKPPESNSNIKMEVGIEDCLHIEFEYNKSKYHLKDVIIGNFYFLLVRIKIKYMEIALI 213

Query: 181 KRESTGSGPNVFNESETLTKFEIMDGAP 264
           KRESTGSGPNVFNESETLTKFEIMDGAP
Sbjct: 214 KRESTGSGPNVFNESETLTKFEIMDGAP 241

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 956,079,753
Number of extensions: 15152519
Number of successful extensions: 30845
Number of sequences better than 10.0: 55
Number of HSP's gapped: 30833
Number of HSP's successfully gapped: 106
Length of query: 272
Length of database: 1,040,966,779
Length adjustment: 126
Effective length of query: 146
Effective length of database: 637,226,869
Effective search space: 93035122874
Effective search space used: 93035122874
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.73  gvh: 0.66  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U10327-1 is cyt
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0