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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10165-1
Contig ID Contig-U10165-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10165-1 (Contig-U10165-1Q) /CSM_Contig/Contig-U10165-1Q.Seq.d
TGTTGGCCTACTGGAATTAGCTTTTTTAAAAAAAAAAATAAACAAACAAC
AAATAACAAAGATATAAATAAATAATTTTTCAATTTAGTTTAAAAAAAAA
GAAAAAACAACATAATGCCTCCACACGGTCATCATCATCATCACCATGGT
CACCATGGTCACCATGAACATATTACAATCACTCCAGTTTATGCACCACC
ACAAGTTGTTCAACCAATTTATGCACCACCACCAGTTGTCCAACCAGTTT
ATGTTCAACCACCAGTAGTTTATCCACAAGCTACAATTGTCTTAGAAACT
GGTCACCACCATGGACATCATCATCACCATGGCCATCATGGTCATCATGA
TCATCACCACCACGGTCATCATGGTCACCACTAAATTATTCAAATAACAA
AAGTTTTCAACAAAAACAAAAATAAAAAAAAAAAACTTTGTATTATAATT
AATTATTACCTAAAATAAAAAAATAATAATAAAAAAAACCTATATTTATT
TT
Gap no gap
Contig length 502
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 1868194
End point 1867690
Strand (PLUS/MINUS) MINUS
Number of clones 6
Number of EST 8
Link to clone list U10165
List of clone(s) 

est1=SFG533Z,1,503
est2=VFN330Z,1,439
est3=SFG533F,15,433
est4=VFN330F,15,436
est5=VSH438Z,18,349
est6=VSC431E,31,280
est7=SSF224E,32,472
est8=SFG526Z,50,492
Translated Amino Acid sequence 
cwptgisffkkknkqttnnkdink*ffnlv*KKRKNNIMPPHGHHHHHHGHHGHHEHITI
TPVYAPPQVVQPIYAPPPVVQPVYVQPPVVYPQATIVLETGHHHGHHHHHGHHGHHDHHH
HGHHGHH*iiqitkvfnknknkkkklciiinyylk*knnnkknlylf
Translated Amino Acid sequence (All Frames) 
Frame A:
cwptgisffkkknkqttnnkdink*ffnlv*KKRKNNIMPPHGHHHHHHGHHGHHEHITI
TPVYAPPQVVQPIYAPPPVVQPVYVQPPVVYPQATIVLETGHHHGHHHHHGHHGHHDHHH
HGHHGHH*iiqitkvfnknknkkkklciiinyylk*knnnkknlylf


Frame B:
vgllelaflkkkinkqqitki*innfsi*fkkkektt*clhtviiiitmvtmvtmnilqs
lqfmhhhklfnqfmhhhqlsnqfmfnhq*fihklqls*klvttmdiiitmaimvimiitt
tvimvttklfk*qkfstktkikkknfvl*liit*nkkiiikktyiyf


Frame C:
laywn*lf*kkk*tnnk*qryk*iifqfslkkkkkqhnastrssssspwspwsp*tyynh
sslctttscstnlctttscptslcsttsslstsynclrnwsppwtssspwpswss*sspp
rsswsplnysnnksfqqkqk*kkktlyyn*llpkikk***kkpifi
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10165-1 (Contig-U10165-1Q)
/CSM_Contig/Contig-U10165-1Q.Seq.d
         (502 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10165-1 (Contig-U10165-1Q) /CSM_Contig/Conti...   422  e-118
Contig-U09353-1 (Contig-U09353-1Q) /CSM_Contig/Conti...    40  0.002
Contig-U13892-1 (Contig-U13892-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U13408-1 (Contig-U13408-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U13284-1 (Contig-U13284-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U12516-1 (Contig-U12516-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U12361-1 (Contig-U12361-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U11908-1 (Contig-U11908-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U11487-1 (Contig-U11487-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U13115-1 (Contig-U13115-1Q) /CSM_Contig/Conti...    32  0.54

>Contig-U10165-1 (Contig-U10165-1Q) /CSM_Contig/Contig-U10165-1Q.Seq.d
          Length = 502

 Score =  422 bits (213), Expect = e-118
 Identities = 213/213 (100%)
 Strand = Plus / Plus

                                                                       
Query: 108 caacataatgcctccacacggtcatcatcatcatcaccatggtcaccatggtcaccatga 167
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 108 caacataatgcctccacacggtcatcatcatcatcaccatggtcaccatggtcaccatga 167

                                                                       
Query: 168 acatattacaatcactccagtttatgcaccaccacaagttgttcaaccaatttatgcacc 227
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 168 acatattacaatcactccagtttatgcaccaccacaagttgttcaaccaatttatgcacc 227

                                                                       
Query: 228 accaccagttgtccaaccagtttatgttcaaccaccagtagtttatccacaagctacaat 287
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 228 accaccagttgtccaaccagtttatgttcaaccaccagtagtttatccacaagctacaat 287

                                            
Query: 288 tgtcttagaaactggtcaccaccatggacatca 320
           |||||||||||||||||||||||||||||||||
Sbjct: 288 tgtcttagaaactggtcaccaccatggacatca 320


 Score = 61.9 bits (31), Expect = 6e-10
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 381 ctaaattattcaaataacaaaagttttcaac 411
           |||||||||||||||||||||||||||||||
Sbjct: 381 ctaaattattcaaataacaaaagttttcaac 411


 Score = 54.0 bits (27), Expect = 1e-07
 Identities = 27/27 (100%)
 Strand = Plus / Plus

                                     
Query: 1  tgttggcctactggaattagctttttt 27
          |||||||||||||||||||||||||||
Sbjct: 1  tgttggcctactggaattagctttttt 27


 Score = 50.1 bits (25), Expect = 2e-06
 Identities = 25/25 (100%)
 Strand = Plus / Plus

                                    
Query: 436 ctttgtattataattaattattacc 460
           |||||||||||||||||||||||||
Sbjct: 436 ctttgtattataattaattattacc 460


 Score = 46.1 bits (23), Expect = 4e-05
 Identities = 32/35 (91%)
 Strand = Plus / Plus

                                              
Query: 133 catcatcatcaccatggtcaccatggtcaccatga 167
           ||||||||||||||||| || |||||||| |||||
Sbjct: 316 catcatcatcaccatggccatcatggtcatcatga 350


 Score = 40.1 bits (20), Expect = 0.002
 Identities = 32/36 (88%)
 Strand = Plus / Plus

                                               
Query: 129 tcatcatcatcatcaccatggtcaccatggtcacca 164
           |||| ||||||| ||||| ||||| |||||||||||
Sbjct: 345 tcatgatcatcaccaccacggtcatcatggtcacca 380


 Score = 28.2 bits (14), Expect = 8.5
 Identities = 14/14 (100%)
 Strand = Plus / Plus

                         
Query: 489 cctatatttatttt 502
           ||||||||||||||
Sbjct: 489 cctatatttatttt 502


>Contig-U09353-1 (Contig-U09353-1Q) /CSM_Contig/Contig-U09353-1Q.Seq.d
          Length = 2520

 Score = 40.1 bits (20), Expect = 0.002
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                
Query: 437  tttgtattataattaattat 456
            ||||||||||||||||||||
Sbjct: 1757 tttgtattataattaattat 1738


>Contig-U13892-1 (Contig-U13892-1Q) /CSM_Contig/Contig-U13892-1Q.Seq.d
          Length = 1339

 Score = 38.2 bits (19), Expect = 0.009
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 129 tcatcatcatcatcaccat 147
           |||||||||||||||||||
Sbjct: 311 tcatcatcatcatcaccat 329


 Score = 28.2 bits (14), Expect = 8.5
 Identities = 14/14 (100%)
 Strand = Plus / Plus

                         
Query: 130 catcatcatcatca 143
           ||||||||||||||
Sbjct: 309 catcatcatcatca 322


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8204
Number of Sequences: 6905
Number of extensions: 8204
Number of successful extensions: 2407
Number of sequences better than 10.0: 960
length of query: 502
length of database: 5,674,871
effective HSP length: 16
effective length of query: 486
effective length of database: 5,564,391
effective search space: 2704294026
effective search space used: 2704294026
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.11.18
Homology vs DNA 
Query= Contig-U10165-1 (Contig-U10165-1Q) /CSM_Contig/Contig-U10165-1Q.Seq.d
         (502 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C84749) Dictyostelium discoideum slug cDNA, clone SSF224.        387   e-113  2
(BJ400665) Dictyostelium discoideum cDNA clone:dds14a09, 3' ...   357   e-113  4
(BJ390912) Dictyostelium discoideum cDNA clone:dds14a09, 5' ...   381   e-113  3
(BJ435522) Dictyostelium discoideum cDNA clone:ddv27l07, 3' ...   381   e-112  2
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...   387   e-109  3
(BJ400672) Dictyostelium discoideum cDNA clone:dds14c07, 3' ...   357   e-103  2
(BJ416810) Dictyostelium discoideum cDNA clone:ddv27l07, 5' ...   345   e-102  3
(AU267451) Dictyostelium discoideum vegetative cDNA clone:VS...   375   1e-99  1
(AU262560) Dictyostelium discoideum vegetative cDNA clone:VS...   188   2e-54  2
(AU262580) Dictyostelium discoideum vegetative cDNA clone:VS...    54   4e-07  2
(AU269142) Dictyostelium discoideum vegetative cDNA clone:VS...    60   5e-07  2
(AU267450) Dictyostelium discoideum vegetative cDNA clone:VS...    60   8e-05  1
(AC181942) Strongylocentrotus purpuratus clone R3-1009C18, W...    58   3e-04  1
(AC180561) Strongylocentrotus purpuratus clone R3-3094K11, W...    58   3e-04  1
(AC179529) Strongylocentrotus purpuratus clone R3-1035B18, W...    58   3e-04  1
(AC133759) Rattus norvegicus clone CH230-5C14, WORKING DRAFT...    56   0.001  1
(AC120819) Rattus norvegicus clone CH230-74L16, *** SEQUENCI...    56   0.001  1
(AC097177) Rattus norvegicus clone CH230-5M14, WORKING DRAFT...    56   0.001  1
(AC146997) Arbacia punctulata clone BAP-144G2, WORKING DRAFT...    54   0.005  1
(FK712712) pl_002-H8 Ilyanassa (snail) 0-48h Ilyanassa obsol...    54   0.005  1
(FC607889) CAXS3571.fwd CAXS Lottia gigantea from head, foot...    50   0.007  2
(AC097024) Rattus norvegicus clone CH230-84J9, WORKING DRAFT...    52   0.013  2
(AC128415) Rattus norvegicus clone CH230-145K4, WORKING DRAF...    52   0.013  2
(AC141921) Rattus norvegicus clone CH230-414K2, WORKING DRAF...    52   0.013  2
(AC098006) Rattus norvegicus clone CH230-104O1, *** SEQUENCI...    52   0.019  1
(EV099625) 0179621 Brassica napus Leaf library Brassica napu...    52   0.019  1
(EV099547) 0179475 Brassica napus Leaf library Brassica napu...    52   0.019  1
(EV016550) BNSCS1CT_UP_095_C11_20APR2007_091 Brassica napus ...    52   0.019  1
(EV015071) BNSCS1CT_UP_068_G11_26MAR2007_083 Brassica napus ...    52   0.019  1
(ES996540) BNAEN3GH_UP_053_H09_02SEP2006_065 Brassica napus ...    52   0.019  1
(ES948185) 49RDOAT_UP_060_B01_27JAN2006_013 Brassica olerace...    52   0.019  1
(ES946698) 49RDOATR_UP_042_B02_25OCT2004_014 Brassica olerac...    52   0.019  1
(ES910003) BNARO6GH_T3_014_C07_07DEC2006_059 Brassica napus ...    52   0.019  1
(EE553835) BNQA_UP_097_C06_02JUN2005_044 Brassica napus BNQA...    52   0.019  1
(EE507553) 24RDBNH_UP_046_B09_28FEB2004_077 Brassica napus 2...    52   0.019  1
(EE485061) DHBN8DCT_UP_010_F03_23FEB2005_021 BRASSICA NAPUS ...    52   0.019  1
(EE463662) BNSCS2CT_UP_014_A09_05JAN2005_079 Brassica napus ...    52   0.019  1
(EE453694) BNBS4DCT_UP_002_H09_24MAR2005_065 Brassica napus ...    52   0.019  1
(EE443604) 72ETGS24_UP_009_B07_19MAY2005_061 72ETGS24 Brassi...    52   0.019  1
(EE441185) 6ETGS6H_UP_025_C05_04FEB2005_043 Brassica napus 6...    52   0.019  1
(EE424599) 31ETGS12_UP_013_H03_07FEB2005_017 Brassica napus ...    52   0.019  1
(DY024886) 53COT2_T3_009_H09_03SEP2004_065 Brassica napus 36...    52   0.019  1
(FG574605) BN24DYSC_UP_045_E11_5FEB2008_087 BN24DYSC Brassic...    52   0.019  1
(FG571728) BN24DYSC_UP_011_A03_31JAN2008_031 BN24DYSC Brassi...    52   0.019  1
(AC140111) Mus musculus BAC clone RP23-16F4 from 12, complet...    38   0.048  2
(AC098769) Rattus norvegicus clone CH230-186P10, *** SEQUENC...    50   0.076  1
(AP007395) Lotus japonicus genomic DNA, chromosome 4, clone:...    50   0.076  1
(AC181624) Strongylocentrotus purpuratus clone R3-1105J9, WO...    50   0.076  1
(AC180890) Strongylocentrotus purpuratus clone R3-3066N13, W...    50   0.076  1
(AC178277) Strongylocentrotus purpuratus clone R3-3093E13, W...    50   0.076  1
(FH741568) CHO_OF5158xb15f1.ab1 CHO_OF5 Nicotiana tabacum ge...    50   0.076  1
(ER434337) 1092963791167 Global-Ocean-Sampling_GS-35-01-01-1...    50   0.076  1
(DT585596) ltu01-30ms1-g20 Ltu01 Liriodendron tulipifera cDN...    50   0.076  1
(FG310574) CG_plate_121H09_CMagister5'Seq Cancer magister Wh...    50   0.076  1
(FG310017) CG_plate_061C03_CMagister5'Seq Cancer magister Wh...    50   0.076  1
(FG309863) CG_plate_045E08_CMagister5'Seq Cancer magister Wh...    50   0.076  1
(FF135954) CJAH-aae62b06.b1 Caenorhabditis_japonica_CJAH1_ES...    50   0.076  1
(FF116391) CJAH-aaa91e09.b1 Caenorhabditis_japonica_CJAH1_ES...    50   0.076  1
(FD496898) Ltu01b_159_F11_C012.g1 Liriodendron Flower Bud Li...    50   0.076  1
(BA000035) Corynebacterium efficiens YS-314 DNA, complete ge...    50   0.076  1
(BU984038) HA31M23r HA Hordeum vulgare subsp. vulgare cDNA c...    44   0.092  2
(AC207294) Macaca mulatta chromosome UNKNOWN clone CH250-513...    48   0.18   2
(EY299335) CAWX14410.rev CAWX Helobdella robusta Primary Ear...    34   0.23   2
(DN135956) tam62h02.y2 Hydra_EST_HyEch_JUMY_T2 Hydractinia e...    36   0.23   2
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    40   0.26   5
(BV728040) ha2498 Helianthus annuus Ha89 genomic fragments H...    48   0.30   1
(AC152823) Mus musculus BAC clone RP24-302N10 from chromosom...    48   0.30   1
(AC147230) Mus musculus BAC clone RP23-239N12 from 15, compl...    48   0.30   1
(AC121930) Mus musculus BAC clone RP24-205C1 from chromosome...    48   0.30   1
(AC117240) Mus musculus BAC clone RP24-140F3 from 12, comple...    48   0.30   1
(AC115037) Mus musculus chromosome 12, clone RP24-276C17, co...    48   0.30   1
(AC188052) Callithrix jacchus chromosome UNK clone CH259-16H...    48   0.30   1
(AM468132) Vitis vinifera contig VV78X019786.4, whole genome...    48   0.30   1
(CS609865) Sequence 310 from Patent EP1795597.                     48   0.30   1
(BD186958) Nucleic acids isolated from neuroblastoma.              48   0.30   1
(BD100893) Novel genes cloned in humanneuroblastoma and frag...    48   0.30   1
(BD020955) Novel gene and novel gene fragment cloned in huma...    48   0.30   1
(AL589790) Human DNA sequence from clone GS1-118I5 on chromo...    48   0.30   1
(AC128425) Rattus norvegicus clone CH230-110N11, WORKING DRA...    48   0.30   1
(AC123286) Rattus norvegicus clone CH230-358L10, *** SEQUENC...    48   0.30   1
(AC112881) Rattus norvegicus clone CH230-51C15, WORKING DRAF...    48   0.30   1
(AC096987) Rattus norvegicus clone CH230-24O20, WORKING DRAF...    48   0.30   1
(CU655937) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.30   1
(CU467792) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.30   1
(CU463901) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.30   1
(AC208285) Nomascus leucogenys leucogenys clone CH271-477F24...    48   0.30   1
(AC197463) Macaca mulatta clone CH250-206P3, WORKING DRAFT S...    48   0.30   1
(AC181286) Strongylocentrotus purpuratus clone R3-4017A08, W...    48   0.30   1
(AC180405) Strongylocentrotus purpuratus clone R3-3009J5, WO...    48   0.30   1
(AC168712) Strongylocentrotus purpuratus clone R3-3011G10, W...    48   0.30   1
(AY325146) Rattus norvegicus Ab1-353 mRNA, complete cds.           48   0.30   1
(AY310155) Rattus norvegicus Ac1292 mRNA, complete cds.            48   0.30   1
(AZ844503) 2M0143P19R Mouse 10kb plasmid UUGC1M library Mus ...    48   0.30   1
(AZ651834) 1M0522E18R Mouse 10kb plasmid UUGC1M library Mus ...    48   0.30   1
(DE926834) Macropus eugenii DNA, BAC clone: MEB1-116B15_F, 5...    48   0.30   1
(CW964500) AIAA-aab91d09.b1 Ancylostoma caninum whole genome...    48   0.30   1
(AG657470) Macaca fuscata fuscata DNA, clone: MSB2-075P03_F,...    48   0.30   1
(EL487529) CHUS2751.b1_M15.ab1 CHU(LMS) puzzle sunflower Hel...    48   0.30   1
(DR350109) 157944 CERES-148 Arabidopsis thaliana cDNA clone ...    48   0.30   1
(DR350108) 1102413 CERES-148 Arabidopsis thaliana cDNA clone...    48   0.30   1

>(C84749) Dictyostelium discoideum slug cDNA, clone SSF224.
          Length = 441

 Score =  387 bits (195), Expect(2) = e-113
 Identities = 195/195 (100%)
 Strand = Plus / Plus


Query: 108 caacataatgcctccacacggtcatcatcatcatcaccatggtcaccatggtcaccatga 167
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 77  caacataatgcctccacacggtcatcatcatcatcaccatggtcaccatggtcaccatga 136


Query: 168 acatattacaatcactccagtttatgcaccaccacaagttgttcaaccaatttatgcacc 227
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 137 acatattacaatcactccagtttatgcaccaccacaagttgttcaaccaatttatgcacc 196


Query: 228 accaccagttgtccaaccagtttatgttcaaccaccagtagtttatccacaagctacaat 287
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 197 accaccagttgtccaaccagtttatgttcaaccaccagtagtttatccacaagctacaat 256


Query: 288 tgtcttagaaactgg 302
           |||||||||||||||
Sbjct: 257 tgtcttagaaactgg 271

 Score = 60.0 bits (30), Expect(2) = e-113
 Identities = 30/30 (100%)
 Strand = Plus / Plus


Query: 382 taaattattcaaataacaaaagttttcaac 411
           ||||||||||||||||||||||||||||||
Sbjct: 351 taaattattcaaataacaaaagttttcaac 380

 Score = 50.1 bits (25), Expect(2) = 3e-05
 Identities = 43/49 (87%)
 Strand = Plus / Plus


Query: 218 tttatgcaccaccaccagttgtccaaccagtttatgttcaaccaccagt 266
           ||||||||||||||| |||||| |||||| ||||||  | |||||||||
Sbjct: 157 tttatgcaccaccacaagttgttcaaccaatttatgcaccaccaccagt 205

 Score = 50.1 bits (25), Expect(2) = 3e-05
 Identities = 43/49 (87%)
 Strand = Plus / Plus


Query: 188 tttatgcaccaccacaagttgttcaaccaatttatgcaccaccaccagt 236
           ||||||||||||||| |||||| |||||| ||||||  | |||||||||
Sbjct: 187 tttatgcaccaccaccagttgtccaaccagtttatgttcaaccaccagt 235

 Score = 34.2 bits (17), Expect(2) = 3e-05
 Identities = 17/17 (100%)
 Strand = Plus / Plus


Query: 150 tcaccatggtcaccatg 166
           |||||||||||||||||
Sbjct: 110 tcaccatggtcaccatg 126

 Score = 34.2 bits (17), Expect(2) = 3e-05
 Identities = 17/17 (100%)
 Strand = Plus / Plus


Query: 141 tcaccatggtcaccatg 157
           |||||||||||||||||
Sbjct: 119 tcaccatggtcaccatg 135

 Score = 46.1 bits (23), Expect = 1.2
 Identities = 32/35 (91%)
 Strand = Plus / Plus


Query: 133 catcatcatcaccatggtcaccatggtcaccatga 167
           ||||||||||||||||| || |||||||| |||||
Sbjct: 285 catcatcatcaccatggccatcatggtcatcatga 319

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 295,434,527
Number of extensions: 22693938
Number of successful extensions: 2768365
Number of sequences better than 10.0: 324
Length of query: 502
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 479
Effective length of database: 93,106,754,628
Effective search space: 44598135466812
Effective search space used: 44598135466812
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10165-1 (Contig-U10165-1Q) /CSM_Contig/Contig-U10165-1Q.Seq.d
         (502 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF009521_1(AF009521|pid:none) Mus spretus sex determining protei...    32   6.5
(Q62563) RecName: Full=Sex-determining region Y protein; AltName...    32   6.5

>AF009521_1(AF009521|pid:none) Mus spretus sex determining protein
           (Sry) gene, complete cds.  &T47235(T47235)
          Length = 355

 Score = 32.3 bits (72), Expect = 6.5
 Identities = 14/30 (46%), Positives = 17/30 (56%)
 Frame = +2

Query: 176 QSLQFMHHHKLFNQFMHHHQLSNQFMFNHQ 265
           Q  QF +HH+   QF  HHQ   QF  +HQ
Sbjct: 146 QQQQFHNHHQQKQQFHDHHQQKQQFHDHHQ 175

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 241,937,655
Number of extensions: 1730476
Number of successful extensions: 3863
Number of sequences better than 10.0: 2
Number of HSP's gapped: 3638
Number of HSP's successfully gapped: 2
Length of query: 167
Length of database: 1,040,966,779
Length adjustment: 119
Effective length of query: 48
Effective length of database: 659,656,864
Effective search space: 31663529472
Effective search space used: 31663529472
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.73  gvh: 0.39  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for Contig-U10165-1 is nuc
VS (DIR, S) 2
VH (FL, L) 0
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 2
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0