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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10137-1
Contig ID Contig-U10137-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10137-1 (Contig-U10137-1Q) /CSM_Contig/Contig-U10137-1Q.Seq.d
AAATTTATTTTAATTTTTTCTTTTATTTTTTAAAATATAAAAAAAAAAGA
AACAAAAATATTTTATAGATGGCAGAATTAACAAATCATTTTAATAAAGT
TAATGAACTTTTAAATGATTGGGATGGAGTTGGATTATCACATGAATTAT
CAATGACAGAATTAATTACCTCAATTTTAAATTCAATAAATCATAATAGT
CATAGTGGTATGAGTAATCAAAAGAAACAACAAATACAAGCAAAAGTTAA
TCAAATTAAAAAGACAGATTTAGTTGTTAAAAGACAACAAGATATAGATA
CATTATGTGCAAATAAAATTCAACACAACTATAATGAAATCGTTGCATAT
CGTTTAAGATCAATTTCATCATTATTAGATTCAAAATATTATGATGCATT
TAGATATTTAATTGAAGCTATAAATTCATTTGTTAAAGTATTTGAATTAT
GGTCACAAAATATATTATGGAGATTATCATTAGATTTACGTTTAATGGCA
GAATTGGCAACTTTAAATGTTGGTAGTAATGATAGTAATAATAGTAATAA
TAATGTTACGATTGATTATTTTGAAGAAGCATCAAGAACATTATTAAGTA
AATGTTTTCAAGCAGCAAATGCTGATAGAACTCCCAAATTTAGCAGAATC
CAAAAAAGAATGCAGCATTAGGAGTTGTAAACCAATTATTTCAAATTTAT
TTTAAAATTAATAATCTTAAACTTTGTAAAAATTTAATTAAAACTATGGA
ATCACCTGGTTTCCCTACATTAGAGAGTTATCCTTTAAATCAAGTTATCA
CATATCGTTTCTTTAATGGTCGTTTATCAGTTTTCAATGGTCAATATAAA
AAAGCACAAGAAGAATTATTATATGCATTTAATAAATGTCCAAATGATTC
AATTAAAAATAAAAGATTAATATTATTATTTTTAGTACCAATGCAATTAG
AACAATGTAAATTCCCAAAGAAATCATTATTAGAGAAATTTAAGTTAACT
CAATTCATTGATATTGTTCAATCTATAAAAAGTGGTAATATTAAACAATT
TAATGAATGTTTGTCAACTCATCAAAACTTTTTCATTTCAAAAGGTATCT
ATTTGATTTTAGAAAAATTGAAAATTATTGTCTATAGAAATCTATTTAAA
AAGGTACATTTAATTACAACTGGTCAAAGAATTCCAATTGGTAATTTCGT
TTCCGCTTTGAAATGGATGGAAAATGATGCTATCGATATCGTATGAAACT
GAATGTATTCTATCAAATTTAATTTATAATGGTTATCTTAAAGGTTATAT
CTCTCATAAAGTTGCTCTTGTTGTCTCTCCAACAAATCCTTTCCCAAAAT
TACCTTTAAATTAAAAACTAAAAAAAATAAAAAAAATAAAAAAAAATAAA
AATAAAAATAATAACAACAAAAGATTAAA
Gap no gap
Contig length 1429
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 1737693
End point 1739123
Strand (PLUS/MINUS) PLUS
Number of clones 6
Number of EST 10
Link to clone list U10137
List of clone(s) 

est1=VFC636F,1,630
est2=VFK513F,28,569
est3=SFG590F,32,643
est4=VFG804E,45,1376
est5=SFD416F,300,555
est6=SFG590Z,637,1365
est7=VFC636Z,638,1411
est8=VFK513Z,657,1375
est9=SFD416Z,715,1429
est10=SFA833Z,1249,1392
Translated Amino Acid sequence 
nlf*ffllffki*kkkkqkyfidgrinksf**s**tfk*lgwswiit*iindrinylnfk
fnks**s*wye*skettntsks*sn*kdrfsc*kttryryimck*nstql**nrcisfki
nfiiirfkil*ci*ifn*sykfic*si*imvtkyimeiiirftfngrignfkcw******
****cyd*lf*rsikniik*mfssskc**nsqi*QNPKKNAALGVVNQLFQIYFKINNLK
LCKNLIKTMESPGFPTLESYPLNQVITYRFFNGRLSVFNGQYKKAQEELLYAFNKCPNDS
IKNKRLILLFLVPMQLEQCKFPKKSLLEKFKLTQFIDIVQSIKSGNIKQFNECLSTHQNF
FISKGIYLILEKLKIIVYRNLFKKVHLITTGQRIPIGNFVSALKWMENDAIDIV*n*mys
ikfnl*wls*rlyls*scscclsnksfpkitfklktkknkknkkk*k*k**qqkik
Translated Amino Acid sequence (All Frames) 
Frame A:
kfilifsfif*nikkketkifyrwqn*qiiliklmnf*migmeldyhmnyq*qn*lpqf*
iq*iiivivv*vikrnnkykqkliklkrqi*llkdnki*ihyvqikfnttimkslhiv*d
qfhhy*iqnimmhldi*lkl*ihllkylnyghkiyygdyh*iyv*wqnwql*mlvvmivi
iviimlrliilkkhqehy*vnvfkqqmlielpnlaeskkecsirsckpiisnlf*n**s*
tl*kfn*nygitwfpyirelsfkssyhisfl*wsfisfqwsi*kstrriiici**msk*f
n*k*kiniiifstnairtm*ipkeiiirei*vnsih*ycsiykkw*y*ti**mfvnsskl
fhfkrylfdfrkienycl*ksi*kgtfnynwsknsnw*frfrfemdgk*cyryrmklnvf
yqi*fimvilkvislikllllslqqilsqnyl*ikn*kk*kk*kkikikiittkd*


Frame B:
nlf*ffllffki*kkkkqkyfidgrinksf**s**tfk*lgwswiit*iindrinylnfk
fnks**s*wye*skettntsks*sn*kdrfsc*kttryryimck*nstql**nrcisfki
nfiiirfkil*ci*ifn*sykfic*si*imvtkyimeiiirftfngrignfkcw******
****cyd*lf*rsikniik*mfssskc**nsqi*QNPKKNAALGVVNQLFQIYFKINNLK
LCKNLIKTMESPGFPTLESYPLNQVITYRFFNGRLSVFNGQYKKAQEELLYAFNKCPNDS
IKNKRLILLFLVPMQLEQCKFPKKSLLEKFKLTQFIDIVQSIKSGNIKQFNECLSTHQNF
FISKGIYLILEKLKIIVYRNLFKKVHLITTGQRIPIGNFVSALKWMENDAIDIV*n*mys
ikfnl*wls*rlyls*scscclsnksfpkitfklktkknkknkkk*k*k**qqkik


Frame C:
iyfnfffyflkykkkrnknil*maeltnhfnkvnellndwdgvglshelsmtelitsiln
sinhnshsgmsnqkkqqiqakvnqikktdlvvkrqqdidtlcankiqhnyneivayrlrs
isslldskyydafrylieainsfvkvfelwsqnilwrlsldlrlmaelatlnvgsndsnn
snnnvtidyfeeasrtllskcfqaanadrtpkfsriqkrmqh*el*tnyfkfilkliiln
fvki*lklwnhlvslh*rvil*iklshivslmvvyqfsmvnikkhkknyymhlinvqmiq
lkikd*yyyf*yqcn*nnvnsqrnhy*rnls*lnslilfnl*kvvilnnlmnvcqliktf
sfqkvsi*f*kn*kllsieiylkryi*lqlvkefqlvisfpl*ngwkmmlsisyetecil
snliyngylkgyishkvalvvsptnpfpklpln*klkkikkikknknknnnnkrl
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10137-1 (Contig-U10137-1Q)
/CSM_Contig/Contig-U10137-1Q.Seq.d
         (1429 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10137-1 (Contig-U10137-1Q) /CSM_Contig/Conti...  1267  0.0
Contig-U06929-1 (Contig-U06929-1Q) /CSM_Contig/Conti...    56  1e-07
Contig-U09397-1 (Contig-U09397-1Q) /CSM_Contig/Conti...    54  4e-07
Contig-U09161-1 (Contig-U09161-1Q) /CSM_Contig/Conti...    54  4e-07
Contig-U12098-1 (Contig-U12098-1Q) /CSM_Contig/Conti...    52  2e-06
Contig-U11842-1 (Contig-U11842-1Q) /CSM_Contig/Conti...    50  7e-06
Contig-U07113-1 (Contig-U07113-1Q) /CSM_Contig/Conti...    50  7e-06
Contig-U14003-1 (Contig-U14003-1Q) /CSM_Contig/Conti...    48  3e-05
Contig-U13980-1 (Contig-U13980-1Q) /CSM_Contig/Conti...    48  3e-05
Contig-U09112-1 (Contig-U09112-1Q) /CSM_Contig/Conti...    48  3e-05

>Contig-U10137-1 (Contig-U10137-1Q) /CSM_Contig/Contig-U10137-1Q.Seq.d
          Length = 1429

 Score = 1267 bits (639), Expect = 0.0
 Identities = 639/639 (100%)
 Strand = Plus / Plus

                                                                       
Query: 49  gaaacaaaaatattttatagatggcagaattaacaaatcattttaataaagttaatgaac 108
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 49  gaaacaaaaatattttatagatggcagaattaacaaatcattttaataaagttaatgaac 108

                                                                       
Query: 109 ttttaaatgattgggatggagttggattatcacatgaattatcaatgacagaattaatta 168
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 109 ttttaaatgattgggatggagttggattatcacatgaattatcaatgacagaattaatta 168

                                                                       
Query: 169 cctcaattttaaattcaataaatcataatagtcatagtggtatgagtaatcaaaagaaac 228
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 169 cctcaattttaaattcaataaatcataatagtcatagtggtatgagtaatcaaaagaaac 228

                                                                       
Query: 229 aacaaatacaagcaaaagttaatcaaattaaaaagacagatttagttgttaaaagacaac 288
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 229 aacaaatacaagcaaaagttaatcaaattaaaaagacagatttagttgttaaaagacaac 288

                                                                       
Query: 289 aagatatagatacattatgtgcaaataaaattcaacacaactataatgaaatcgttgcat 348
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 289 aagatatagatacattatgtgcaaataaaattcaacacaactataatgaaatcgttgcat 348

                                                                       
Query: 349 atcgtttaagatcaatttcatcattattagattcaaaatattatgatgcatttagatatt 408
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 349 atcgtttaagatcaatttcatcattattagattcaaaatattatgatgcatttagatatt 408

                                                                       
Query: 409 taattgaagctataaattcatttgttaaagtatttgaattatggtcacaaaatatattat 468
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 409 taattgaagctataaattcatttgttaaagtatttgaattatggtcacaaaatatattat 468

                                                                       
Query: 469 ggagattatcattagatttacgtttaatggcagaattggcaactttaaatgttggtagta 528
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 469 ggagattatcattagatttacgtttaatggcagaattggcaactttaaatgttggtagta 528

                                                                       
Query: 529 atgatagtaataatagtaataataatgttacgattgattattttgaagaagcatcaagaa 588
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 529 atgatagtaataatagtaataataatgttacgattgattattttgaagaagcatcaagaa 588

                                                                       
Query: 589 cattattaagtaaatgttttcaagcagcaaatgctgatagaactcccaaatttagcagaa 648
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 589 cattattaagtaaatgttttcaagcagcaaatgctgatagaactcccaaatttagcagaa 648

                                                  
Query: 649 tccaaaaaagaatgcagcattaggagttgtaaaccaatt 687
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 649 tccaaaaaagaatgcagcattaggagttgtaaaccaatt 687


 Score = 1227 bits (619), Expect = 0.0
 Identities = 619/619 (100%)
 Strand = Plus / Plus

                                                                        
Query: 744  ctatggaatcacctggtttccctacattagagagttatcctttaaatcaagttatcacat 803
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 744  ctatggaatcacctggtttccctacattagagagttatcctttaaatcaagttatcacat 803

                                                                        
Query: 804  atcgtttctttaatggtcgtttatcagttttcaatggtcaatataaaaaagcacaagaag 863
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 804  atcgtttctttaatggtcgtttatcagttttcaatggtcaatataaaaaagcacaagaag 863

                                                                        
Query: 864  aattattatatgcatttaataaatgtccaaatgattcaattaaaaataaaagattaatat 923
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 864  aattattatatgcatttaataaatgtccaaatgattcaattaaaaataaaagattaatat 923

                                                                        
Query: 924  tattatttttagtaccaatgcaattagaacaatgtaaattcccaaagaaatcattattag 983
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 924  tattatttttagtaccaatgcaattagaacaatgtaaattcccaaagaaatcattattag 983

                                                                        
Query: 984  agaaatttaagttaactcaattcattgatattgttcaatctataaaaagtggtaatatta 1043
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 984  agaaatttaagttaactcaattcattgatattgttcaatctataaaaagtggtaatatta 1043

                                                                        
Query: 1044 aacaatttaatgaatgtttgtcaactcatcaaaactttttcatttcaaaaggtatctatt 1103
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1044 aacaatttaatgaatgtttgtcaactcatcaaaactttttcatttcaaaaggtatctatt 1103

                                                                        
Query: 1104 tgattttagaaaaattgaaaattattgtctatagaaatctatttaaaaaggtacatttaa 1163
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1104 tgattttagaaaaattgaaaattattgtctatagaaatctatttaaaaaggtacatttaa 1163

                                                                        
Query: 1164 ttacaactggtcaaagaattccaattggtaatttcgtttccgctttgaaatggatggaaa 1223
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1164 ttacaactggtcaaagaattccaattggtaatttcgtttccgctttgaaatggatggaaa 1223

                                                                        
Query: 1224 atgatgctatcgatatcgtatgaaactgaatgtattctatcaaatttaatttataatggt 1283
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1224 atgatgctatcgatatcgtatgaaactgaatgtattctatcaaatttaatttataatggt 1283

                                                                        
Query: 1284 tatcttaaaggttatatctctcataaagttgctcttgttgtctctccaacaaatcctttc 1343
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1284 tatcttaaaggttatatctctcataaagttgctcttgttgtctctccaacaaatcctttc 1343

                               
Query: 1344 ccaaaattacctttaaatt 1362
            |||||||||||||||||||
Sbjct: 1344 ccaaaattacctttaaatt 1362


 Score = 42.1 bits (21), Expect = 0.002
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                 
Query: 1409 taataacaacaaaagattaaa 1429
            |||||||||||||||||||||
Sbjct: 1409 taataacaacaaaagattaaa 1429


>Contig-U06929-1 (Contig-U06929-1Q) /CSM_Contig/Contig-U06929-1Q.Seq.d
          Length = 726

 Score = 56.0 bits (28), Expect = 1e-07
 Identities = 31/32 (96%)
 Strand = Plus / Plus

                                           
Query: 524 tagtaatgatagtaataatagtaataataatg 555
           ||||||| ||||||||||||||||||||||||
Sbjct: 341 tagtaataatagtaataatagtaataataatg 372


>Contig-U09397-1 (Contig-U09397-1Q) /CSM_Contig/Contig-U09397-1Q.Seq.d
          Length = 1061

 Score = 54.0 bits (27), Expect = 4e-07
 Identities = 39/43 (90%)
 Strand = Plus / Plus

                                                      
Query: 512 tttaaatgttggtagtaatgatagtaataatagtaataataat 554
           |||||||| | |||||||| ||| |||||||||||||||||||
Sbjct: 797 tttaaatgatagtagtaataataataataatagtaataataat 839


 Score = 34.2 bits (17), Expect = 0.40
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 538 ataatagtaataataat 554
           |||||||||||||||||
Sbjct: 754 ataatagtaataataat 770


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 33,471
Number of Sequences: 6905
Number of extensions: 33471
Number of successful extensions: 5907
Number of sequences better than 10.0: 1108
length of query: 1429
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1413
effective length of database: 5,564,391
effective search space: 7862484483
effective search space used: 7862484483
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.18
Homology vs DNA 
Query= Contig-U10137-1 (Contig-U10137-1Q) /CSM_Contig/Contig-U10137-1Q.Seq.d
         (1429 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ400781) Dictyostelium discoideum cDNA clone:dds14c23, 3' ...  1219   0.0    1
(BJ413072) Dictyostelium discoideum cDNA clone:ddv14h02, 5' ...  1130   0.0    3
(BJ391037) Dictyostelium discoideum cDNA clone:dds14c23, 5' ...  1122   0.0    1
(BJ411923) Dictyostelium discoideum cDNA clone:ddv6g10, 5' e...  1110   0.0    1
(BJ433510) Dictyostelium discoideum cDNA clone:ddv22i03, 3' ...   987   0.0    2
(BJ431410) Dictyostelium discoideum cDNA clone:ddv14h02, 3' ...   987   0.0    2
(BJ399729) Dictyostelium discoideum cDNA clone:dds7p04, 3' e...   987   0.0    2
(BJ430173) Dictyostelium discoideum cDNA clone:ddv6g10, 3' e...   979   0.0    2
(BJ347213) Dictyostelium discoideum cDNA clone:dda26n09, 3' ...   979   0.0    2
(BJ415296) Dictyostelium discoideum cDNA clone:ddv22i03, 5' ...   995   0.0    1
(BJ329830) Dictyostelium discoideum cDNA clone:dda26n09, 5' ...   878   0.0    3
(BJ387975) Dictyostelium discoideum cDNA clone:dds7p04, 5' e...   496   e-135  1
(BJ398787) Dictyostelium discoideum cDNA clone:dds2b10, 3' e...   182   2e-41  1
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    56   5e-07  12
(AL844509) Plasmodium falciparum chromosome 13.                    48   4e-06  9
(AE001384) Plasmodium falciparum 3D7 chromosome 2 section 21...    46   7e-06  4
(CR382401) Plasmodium falciparum chromosome 6, complete sequ...    42   2e-05  19
(AC178448) Strongylocentrotus purpuratus clone R3-1118G21, W...    46   5e-05  7
(AE014841) Plasmodium falciparum 3D7 chromosome 11 section 6...    46   6e-05  15
(AE014849) Plasmodium falciparum 3D7 chromosome 12, section ...    36   2e-04  14
(AC115581) Dictyostelium discoideum chromosome 2 map complem...    48   2e-04  7
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    52   4e-04  9
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    56   9e-04  9
(EX835152) CBNB4940.fwd CBNB Phycomyces blakesleeanus NRRL15...    54   0.001  2
(BM294064) jh_50671.z1 Penaeus chinensis' cephalothorax cDNA...    58   0.001  1
(AE014850) Plasmodium falciparum 3D7 chromosome 12, section ...    50   0.001  15
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    50   0.001  11
(CU861474) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    48   0.002  9
(AC213924) Populus trichocarpa clone POP042-A21, complete se...    38   0.002  9
(AC115605) Dictyostelium discoideum chromosome 2 map 5817431...    50   0.002  3
(CR762436) Zebrafish DNA sequence from clone DKEY-261M9.           40   0.002  10
(AC182671) Populus trichocarpa clone Pop1-24F1, complete seq...    38   0.003  8
(BX950859) Zebrafish DNA sequence from clone CH211-134M1 in ...    48   0.003  4
(AC168386) Strongylocentrotus purpuratus clone R3-4013A3, WO...    42   0.003  5
(CR847928) Zebrafish DNA sequence from clone DKEYP-67E7 in l...    56   0.004  1
(CQ595565) Sequence 23323 from Patent WO0171042.                   56   0.004  1
(AR524020) Sequence 28980 from patent US 6703491.                  56   0.004  1
(AR508127) Sequence 13087 from patent US 6703491.                  56   0.004  1
(AE013599) Drosophila melanogaster chromosome 2R, complete s...    56   0.004  1
(AC115604) Dictyostelium discoideum chromosome 2 map 4354771...    56   0.004  1
(AC007054) Drosophila melanogaster, chromosome 2R, region 41...    56   0.004  1
(AC141791) Apis mellifera clone CH224-59E3, WORKING DRAFT SE...    56   0.004  1
(AC120208) Canis familiaris clone RP81-61N24, WORKING DRAFT ...    56   0.004  1
(AC120206) Canis familiaris clone RP81-219C14, WORKING DRAFT...    56   0.004  1
(AC188537) Canis lupus familiaris chromosome 31 clone XX-364...    56   0.004  1
(AC176748) Strongylocentrotus purpuratus clone R3-3097G9, WO...    56   0.004  1
(AC017137) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    56   0.004  1
(FH612397) CHO_OF4937xk19f1.ab1 CHO_OF4 Nicotiana tabacum ge...    56   0.004  1
(EJ463357) 1095383000298 Global-Ocean-Sampling_GS-28-01-01-1...    56   0.004  1
(ED121292) AUAC-aal24h11.b1 Ascaris suum whole genome shotgu...    56   0.004  1
(EC198638) 185740 CK01 Drosophila melanogaster cDNA clone 17...    56   0.004  1
(DW282483) UI-S-HH0-adj-j-11-0-UI.s1 UI-S-HH0 Euprymna scolo...    56   0.004  1
(AU268347) Dictyostelium discoideum vegetative cDNA clone:VS...    56   0.004  1
(AU268346) Dictyostelium discoideum vegetative cDNA clone:VS...    56   0.004  1
(CO321119) EK290339.5prime Exelixis FlyTag CK01 pCDNA-SK+ Dr...    56   0.004  1
(CO278195) EK129313.5prime Exelixis FlyTag CK01 pCDNA-SK+ Dr...    56   0.004  1
(AC114258) Dictyostelium discoideum chromosome 2 map complem...    40   0.004  7
(BZ316872) ia77f09.b1 WGS-ZmaysF (JM107 adapted methyl filte...    54   0.005  2
(AG601304) Mus musculus molossinus DNA, clone:MSMg01-533F20....    54   0.005  2
(CG324073) OGVCT01TV ZM_0.7_1.5_KB Zea mays genomic clone ZM...    54   0.005  2
(AJ011856) Saccharomyces cerevisiae complete mitochondrial g...    44   0.006  8
(CP000084) Candidatus Pelagibacter ubique HTCC1062, complete...    36   0.006  22
(AC161241) Medicago truncatula clone mth2-193c3, complete se...    38   0.006  9
(EY324829) CAWY10532.rev CAWY Helobdella robusta Subtracted ...    54   0.006  2
(AX345916) Sequence 987 from Patent WO0200928.                     36   0.006  6
(AM434438) Vitis vinifera contig VV78X055195.11, whole genom...    36   0.008  5
(AC115598) Dictyostelium discoideum chromosome 2 map 581427-...    40   0.008  10
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...    46   0.012  8
(AC006279) Plasmodium falciparum chromosome 12 clone 3D7, **...    46   0.012  12
(DH139579) Oryzias latipes Fosmid clone:GOLWFno401_o07, forw...    48   0.013  2
(DX990333) CHORI103-11E14.TV CHORI103 Schistosoma mansoni ge...    44   0.013  2
(AL929352) Plasmodium falciparum strain 3D7, chromosome 5, s...    50   0.013  14
(AC116919) Dictyostelium discoideum chromosome 2 map complem...    50   0.014  6
(CP000102) Methanosphaera stadtmanae DSM 3091, complete genome.    38   0.014  21
(AE014830) Plasmodium falciparum 3D7 chromosome 10 section 2...    38   0.014  12
(AC005505) Plasmodium falciparum chromosome 12 clone 3D7, **...    50   0.014  11
(AE014848) Plasmodium falciparum 3D7 chromosome 12, section ...    44   0.014  12
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    54   0.015  13
(AL844506) Plasmodium falciparum chromosome  7.                    48   0.015  12
(CR385035) Zebrafish DNA sequence from clone DKEY-182I1 in l...    54   0.015  1
(BX927112) Zebrafish DNA sequence from clone DKEY-109M11 in ...    54   0.015  1
(AL954383) Zebrafish DNA sequence from clone CH211-226M11 in...    54   0.015  1
(AL935289) Zebrafish DNA sequence from clone CH211-232C12.         54   0.015  1
(AL928986) Mouse DNA sequence from clone RP23-477P12 on chro...    54   0.015  1
(AL844139) Mouse DNA sequence from clone RP23-21G2 on chromo...    54   0.015  1
(AP009604) Solanum lycopersicum DNA, chromosome 8, clone: C0...    54   0.015  1
(AP006130) Lotus japonicus genomic DNA, chromosome 3, clone:...    54   0.015  1
(AC182680) Populus trichocarpa clone Pop1-30O8, complete seq...    54   0.015  1
(FM162572) Trypanosoma brucei Lister 427 surface glycoprotei...    54   0.015  1
(AY170439) Dictyostelium discoideum mitochondrial DNA polyme...    54   0.015  1
(AY160094) Dictyostelium discoideum nucleotide exchange fact...    54   0.015  1
(AF088979) Dictyostelium discoideum beige protein homolog (l...    54   0.015  1
(AE001425) Plasmodium falciparum 3D7 chromosome 2 section 62...    54   0.015  1
(AC115612) Dictyostelium discoideum chromosome 2 map 6245135...    54   0.015  1
(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...    54   0.015  1
(AC141794) Apis mellifera clone CH224-60D2, WORKING DRAFT SE...    54   0.015  1
(AC136579) Rattus norvegicus clone CH230-11I20, WORKING DRAF...    54   0.015  1
(AC131125) Rattus norvegicus clone CH230-4G22, WORKING DRAFT...    54   0.015  1
(AC128506) Rattus norvegicus clone CH230-33B7, *** SEQUENCIN...    54   0.015  1
(AC126636) Rattus norvegicus clone CH230-50A17, WORKING DRAF...    54   0.015  1

>(BJ400781) Dictyostelium discoideum cDNA clone:dds14c23, 3' end,
            single read.
          Length = 729

 Score = 1219 bits (615), Expect = 0.0
 Identities = 618/619 (99%)
 Strand = Plus / Minus


Query: 744  ctatggaatcacctggtttccctacattagagagttatcctttaaatcaagttatcacat 803
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 623  ctatggaatcacctggtttccctacattagagagttatcctttaaatcaagttatcacat 564


Query: 804  atcgtttctttaatggtcgtttatcagttttcaatggtcaatataaaaaagcacaagaag 863
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 563  atcgtttctttaatggtcgtttatcagttttcaatggtcaatataaaaaagcacaagaag 504


Query: 864  aattattatatgcatttaataaatgtccaaatgattcaattaaaaataaaagattaatat 923
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 503  aattattatatgcatttaataaatgtccaaatgattcaattaaaaataaaagattaatat 444


Query: 924  tattatttttagtaccaatgcaattagaacaatgtaaattcccaaagaaatcattattag 983
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 443  tattatttttagtaccaatgcaattagaacaatgtaaattcccaaagaaatcattattag 384


Query: 984  agaaatttaagttaactcaattcattgatattgttcaatctataaaaagtggtaatatta 1043
            || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 383  aggaatttaagttaactcaattcattgatattgttcaatctataaaaagtggtaatatta 324


Query: 1044 aacaatttaatgaatgtttgtcaactcatcaaaactttttcatttcaaaaggtatctatt 1103
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 323  aacaatttaatgaatgtttgtcaactcatcaaaactttttcatttcaaaaggtatctatt 264


Query: 1104 tgattttagaaaaattgaaaattattgtctatagaaatctatttaaaaaggtacatttaa 1163
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 263  tgattttagaaaaattgaaaattattgtctatagaaatctatttaaaaaggtacatttaa 204


Query: 1164 ttacaactggtcaaagaattccaattggtaatttcgtttccgctttgaaatggatggaaa 1223
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 203  ttacaactggtcaaagaattccaattggtaatttcgtttccgctttgaaatggatggaaa 144


Query: 1224 atgatgctatcgatatcgtatgaaactgaatgtattctatcaaatttaatttataatggt 1283
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 143  atgatgctatcgatatcgtatgaaactgaatgtattctatcaaatttaatttataatggt 84


Query: 1284 tatcttaaaggttatatctctcataaagttgctcttgttgtctctccaacaaatcctttc 1343
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 83   tatcttaaaggttatatctctcataaagttgctcttgttgtctctccaacaaatcctttc 24


Query: 1344 ccaaaattacctttaaatt 1362
            |||||||||||||||||||
Sbjct: 23   ccaaaattacctttaaatt 5

 Score = 54.0 bits (27), Expect(2) = 1e-06
 Identities = 27/27 (100%)
 Strand = Plus / Minus


Query: 651 caaaaaagaatgcagcattaggagttg 677
           |||||||||||||||||||||||||||
Sbjct: 716 caaaaaagaatgcagcattaggagttg 690

 Score = 32.2 bits (16), Expect(2) = 1e-06
 Identities = 19/20 (95%)
 Strand = Plus / Minus


Query: 637 aatttagcagaatccaaaaa 656
           |||||||||||||| |||||
Sbjct: 729 aatttagcagaatcaaaaaa 710

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,778,630,342
Number of extensions: 117449129
Number of successful extensions: 10767737
Number of sequences better than 10.0: 2327
Length of query: 1429
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1405
Effective length of database: 97,308,875,965
Effective search space: 136718970730825
Effective search space used: 136718970730825
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10137-1 (Contig-U10137-1Q) /CSM_Contig/Contig-U10137-1Q.Seq.d
         (1429 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PX7) RecName: Full=PCI domain-containing protein 2 homolog; ...   394   0.0
(Q5FWP8) RecName: Full=PCI domain-containing protein 2; AltName:...   174   2e-56
BC006047_1(BC006047|pid:none) Mus musculus PCI domain containing...   172   2e-53
(Q8BFV2) RecName: Full=PCI domain-containing protein 2; AltName:...   172   2e-53
BC031246_1(BC031246|pid:none) Homo sapiens PCI domain containing...   171   2e-53
AL137002_17(AL137002|pid:none) Human DNA sequence from clone RP1...   171   8e-53
BT074722_1(BT074722|pid:none) Osmerus mordax clone omor-rgc-513-...   172   1e-52
AK001302_1(AK001302|pid:none) Homo sapiens cDNA FLJ10440 fis, cl...   169   4e-52
(Q2TBN6) RecName: Full=PCI domain-containing protein 2; AltName:...   168   7e-52
AL136221_3(AL136221|pid:none) Human DNA sequence from clone RP11...   171   2e-49
BT058705_1(BT058705|pid:none) Salmo salar clone Contig3580 PCI d...   176   5e-49
AK002167_1(AK002167|pid:none) Homo sapiens cDNA FLJ11305 fis, cl...   169   8e-49
BT049241_1(BT049241|pid:none) Salmo salar clone ssal-eve-503-255...   174   1e-48
AK000888_1(AK000888|pid:none) Homo sapiens cDNA FLJ10026 fis, cl...   171   3e-48
AL136221_1(AL136221|pid:none) Human DNA sequence from clone RP11...   171   3e-48
CP001327_560(CP001327|pid:none) Micromonas sp. RCC299 chromosome...   180   9e-48
(Q9VTL1) RecName: Full=PCI domain-containing protein 2 homolog; ...   169   3e-47
CP001141_282(CP001141|pid:none) Phaeodactylum tricornutum CCAP 1...   161   2e-45
FN357416_35(FN357416|pid:none) Schistosoma mansoni genome sequen...   163   1e-44
(Q60YJ7) RecName: Full=PCI domain-containing protein 2 homolog; ...   156   1e-40
(Q95QU0) RecName: Full=PCI domain-containing protein 2 homolog; ...   155   5e-40
T15664(T15664)hypothetical protein C27F2.7 - Caenorhabditis eleg...   155   4e-36
(Q5KAS8) RecName: Full=Protein CSN12 homolog;  &AE017350_60(AE01...   113   1e-31
EU960631_1(EU960631|pid:none) Zea mays clone 226763 unknown mRNA.     113   5e-28
(Q4WJX0) RecName: Full=Protein CSN12 homolog;                         108   3e-27
(Q4IMN9) Protein CSN12 homolog.                                       102   4e-24
(O13873) RecName: Full=Protein CSN12 homolog;  &CU329670_2044(CU...   100   4e-23
(Q6C1L4) RecName: Full=Protein CSN12 homolog;  &CR382132_636(CR3...   101   8e-22
CU633438_418(CU633438|pid:none) Podospora anserina genomic DNA c...   102   3e-21
AJ719547_1(AJ719547|pid:none) Gallus gallus mRNA for hypothetica...    79   5e-18
AY813561_1(AY813561|pid:none) Schistosoma japonicum SJCHGC01789 ...    95   5e-18
CR380955_345(CR380955|pid:none) Candida glabrata strain CBS138 c...    89   3e-16
CR382127_321(CR382127|pid:none) Yarrowia lipolytica strain CLIB1...    82   6e-14
CR382139_1153(CR382139|pid:none) Debaryomyces hansenii chromosom...    79   3e-13
CR382124_148(CR382124|pid:none) Kluyveromyces lactis strain NRRL...    77   1e-12
FN392319_535(FN392319|pid:none) Pichia pastoris GS115 chromosome...    77   1e-12
CU928179_716(CU928179|pid:none) Zygosaccharomyces rouxii strain ...    57   1e-11
CU638744_483(CU638744|pid:none) Podospora anserina genomic DNA c...    73   2e-11
CP000496_674(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...    71   1e-10
CR382134_507(CR382134|pid:none) Debaryomyces hansenii strain CBS...    71   1e-10
(Q08231) RecName: Full=Nuclear mRNA export protein THP1; AltName...    70   2e-10
CU928167_352(CU928167|pid:none) Kluyveromyces thermotolerans str...    67   2e-09
(Q6CPB1) RecName: Full=COP9 signalosome complex subunit 12;  &CR...    47   1e-08
CP000499_384(CP000499|pid:none) Pichia stipitis CBS 6054 chromos...    61   9e-08
(Q1ZXD3) RecName: Full=26S proteasome non-ATPase regulatory subu...    56   4e-06
AM494972_285(AM494972|pid:none) Leishmania braziliensis chromoso...    55   5e-06
AC005917_15(AC005917|pid:none) Arabidopsis thaliana chromosome 2...    49   4e-04
FN357663_10(FN357663|pid:none) Schistosoma mansoni genome sequen...    49   6e-04
FN357663_9(FN357663|pid:none) Schistosoma mansoni genome sequenc...    49   6e-04
AL022117_1(AL022117|pid:none) S.pombe chromosome II cosmid c119....    48   0.001
(O42897) RecName: Full=Probable 26S proteasome regulatory subuni...    48   0.001
BC073593_1(BC073593|pid:none) Xenopus laevis MGC82894 protein, m...    47   0.002
BT059067_1(BT059067|pid:none) Salmo salar clone ssal-rgf-523-284...    47   0.002
BT043859_1(BT043859|pid:none) Salmo salar clone HM5_0753 proteas...    47   0.002
BT043858_1(BT043858|pid:none) Salmo salar clone HM4_3606 proteas...    47   0.002
AJ719445_1(AJ719445|pid:none) Gallus gallus mRNA for hypothetica...    46   0.003
CU928170_322(CU928170|pid:none) Kluyveromyces thermotolerans str...    45   0.008
AK094206_1(AK094206|pid:none) Homo sapiens cDNA FLJ36887 fis, cl...    45   0.008
AK300081_1(AK300081|pid:none) Homo sapiens cDNA FLJ54148 complet...    45   0.008
AK145467_1(AK145467|pid:none) Mus musculus cDNA, RIKEN full-leng...    45   0.008
AK145488_1(AK145488|pid:none) Mus musculus blastocyst blastocyst...    45   0.008
D67025_1(D67025|pid:none) Homo sapiens mRNA for proteasome subun...    45   0.008
AB169643_1(AB169643|pid:none) Macaca fascicularis brain cDNA, cl...    45   0.008
AK298411_1(AK298411|pid:none) Homo sapiens cDNA FLJ55739 complet...    45   0.008
AK054611_1(AK054611|pid:none) Homo sapiens cDNA FLJ30049 fis, cl...    45   0.008
I49504(I49504) Tum-P91A antigen - mouse  &M25149_1(M25149|pid:no...    45   0.008
BC085881_1(BC085881|pid:none) Rattus norvegicus proteasome (pros...    45   0.008
AY891635_1(AY891635|pid:none) Synthetic construct Homo sapiens c...    45   0.008
CR456772_1(CR456772|pid:none) Homo sapiens full open reading fra...    45   0.008
AK077252_1(AK077252|pid:none) Mus musculus 11 days pregnant adul...    45   0.008
(O43242) RecName: Full=26S proteasome non-ATPase regulatory subu...    45   0.008
AK293539_1(AK293539|pid:none) Homo sapiens cDNA FLJ56054 complet...    44   0.011
(Q75BU2) RecName: Full=COP9 signalosome complex subunit 12;  &AE...    44   0.019
AK153505_1(AK153505|pid:none) Mus musculus bone marrow macrophag...    44   0.019
BC012302_1(BC012302|pid:none) Homo sapiens proteasome (prosome, ...    44   0.019
AL590445_157(AL590445|pid:none) chromosome V of strain GB-M1 of ...    42   0.055
AY089337_1(AY089337|pid:none) Drosophila melanogaster AT15146 fu...    40   0.21
(Q9U5Z8) RecName: Full=Probable 26S proteasome non-ATPase regula...    40   0.27
AL391737_83(AL391737|pid:none) chromosome I of strain GB-M1 of E...    29   1.1
CP000716_351(CP000716|pid:none) Thermosipho melanesiensis BI429,...    37   1.8
AM502242_56(AM502242|pid:none) Leishmania infantum chromosome 24.      37   2.3
CU633865_198(CU633865|pid:none) Podospora anserina genomic DNA c...    35   5.1
CP001147_671(CP001147|pid:none) Thermodesulfovibrio yellowstonii...    35   5.1
AM910994_54(AM910994|pid:none) Plasmodium knowlesi strain H chro...    35   8.7
AF517882_1(AF517882|pid:none) Griffithsia japonica isolate Gj511...    35   8.7

>(Q54PX7) RecName: Full=PCI domain-containing protein 2 homolog;
            AltName: Full=CSN12-like protein;
          Length = 430

 Score =  394 bits (1011), Expect(3) = 0.0
 Identities = 196/196 (100%), Positives = 196/196 (100%)
 Frame = +2

Query: 653  KKNAALGVVNQLFQIYFKINNLKLCKNLIKTMESPGFPTLESYPLNQVITYRFFNGRLSV 832
            KKNAALGVVNQLFQIYFKINNLKLCKNLIKTMESPGFPTLESYPLNQVITYRFFNGRLSV
Sbjct: 195  KKNAALGVVNQLFQIYFKINNLKLCKNLIKTMESPGFPTLESYPLNQVITYRFFNGRLSV 254

Query: 833  FNGQYKKAQEELLYAFNKCPNDSIKNKRLILLFLVPMQLEQCKFPKKSLLEKFKLTQFID 1012
            FNGQYKKAQEELLYAFNKCPNDSIKNKRLILLFLVPMQLEQCKFPKKSLLEKFKLTQFID
Sbjct: 255  FNGQYKKAQEELLYAFNKCPNDSIKNKRLILLFLVPMQLEQCKFPKKSLLEKFKLTQFID 314

Query: 1013 IVQSIKSGNIKQFNECLSTHQNFFISKGIYLILEKLKIIVYRNLFKKVHLITTGQRIPIG 1192
            IVQSIKSGNIKQFNECLSTHQNFFISKGIYLILEKLKIIVYRNLFKKVHLITTGQRIPIG
Sbjct: 315  IVQSIKSGNIKQFNECLSTHQNFFISKGIYLILEKLKIIVYRNLFKKVHLITTGQRIPIG 374

Query: 1193 NFVSALKWMENDAIDI 1240
            NFVSALKWMENDAIDI
Sbjct: 375  NFVSALKWMENDAIDI 390

 Score =  319 bits (817), Expect(3) = 0.0
 Identities = 165/196 (84%), Positives = 167/196 (85%)
 Frame = +3

Query: 69  MAELTNHFNKVNELLNDWDGVGLSHELSMTEXXXXXXXXXXXXXXXGMSNQKKQQIQAKV 248
           MAELTNHFNKVNELLNDWDGVGLSHELSMTE               GMSNQKKQQIQAKV
Sbjct: 1   MAELTNHFNKVNELLNDWDGVGLSHELSMTELITSILNSINHNSHSGMSNQKKQQIQAKV 60

Query: 249 NQIKKTDLVVKRQQDIDTLCANKIQHNYNEIVAYRLRSISSLLDSKYYDAFRYLIEAINS 428
           NQIKKTDLVVKRQQDIDTLCANKIQHNYNEIVAYRLRSISSLLDSKYYDAFRYLIEAINS
Sbjct: 61  NQIKKTDLVVKRQQDIDTLCANKIQHNYNEIVAYRLRSISSLLDSKYYDAFRYLIEAINS 120

Query: 429 FVKVFELWSQNILWRLSLDLRLMAELATLNVGXXXXXXXXXXVTIDYFEEASRTLLSKCF 608
           FVKVFELWSQNILWRLSLDLRLMAELATLNVG          VTIDYFEEASRTLLSKCF
Sbjct: 121 FVKVFELWSQNILWRLSLDLRLMAELATLNVGSNDSNNSNNNVTIDYFEEASRTLLSKCF 180

Query: 609 QAANADRTPKFSRIQK 656
           QAANADRTP  +  +K
Sbjct: 181 QAANADRTPNLAESKK 196

 Score = 71.6 bits (174), Expect(3) = 0.0
 Identities = 33/33 (100%), Positives = 33/33 (100%)
 Frame = +3

Query: 1245 ETECILSNLIYNGYLKGYISHKVALVVSPTNPF 1343
            ETECILSNLIYNGYLKGYISHKVALVVSPTNPF
Sbjct: 392  ETECILSNLIYNGYLKGYISHKVALVVSPTNPF 424

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,685,906,080
Number of extensions: 28745591
Number of successful extensions: 79854
Number of sequences better than 10.0: 85
Number of HSP's gapped: 79489
Number of HSP's successfully gapped: 132
Length of query: 476
Length of database: 1,040,966,779
Length adjustment: 132
Effective length of query: 344
Effective length of database: 618,001,159
Effective search space: 212592398696
Effective search space used: 212592398696
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.82  gvh: 0.37  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U10137-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 3
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 3
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0