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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U10099-1
Contig ID Contig-U10099-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U10099-1 (Contig-U10099-1Q) /CSM_Contig/Contig-U10099-1Q.Seq.d
TAATCGATAATTTAATTTATTTATACTCTAACACTTAAAGTTCTTTGTTA
CATGGACAGTAAGTACCATAGCATTTATCAACTTTAACTGCCATGCCACT
ACCTGAACCGCTACCACCACCACTTCCAGAACTGCCACCACTTCCAGAAC
TACCACCGCTACTTCCACTACTTCCACTGCTTCCACTACTTCCACTGCTA
CCACTTGAAGCAGTTGATTGACCAGTTTGGGCACCAGTTTGACCTGAGGT
TTGACCTGAGGTTTGGGTACCTGATTGTGAACCTGATTGTTGTCCTGATT
GTGAACCTGATTGTGAACTTGTTTGTTGTCCTGATTGTGAACCTGATTGC
GAACCTGTTTGTTGTCCTGATTGTGAACCCGATGTTTGTGAACCTGATGT
TTGTGAACCTGAGGTTTGGGAACCTGAAGTTTGGGTGCTTGAAGATGAAG
TTTTACCAGTTGATGATGGACCAAGATGATGAAGCTTTACCAGTTGATGA
TGGACCTGAAGATGAAGCTTTACCAGTTGATGATGGACCACTATGATGAA
GCTCTACCAGTTGTTGATGGACCTGATGATGAAGCTCTACCAGTTGTTGA
TGGACCTGATGATGAAGCTCTACCGGGTTGTTGATGGGACCAAGATGATG
AGGCTTTACCAGTTGTTGTTGAACGCTGAGGTAGTTGATGTTGTTGTTGG
AGTTGGTTGACAACCGAAAATAGTTCCATTTGTACATTTACAAGTGCAAG
TTTGTAAATCACATGGAATAGTATAAACTTCTTGAATAATGGAATTACAT
CTTTCCATACAATGAGATTTATCTTTTACAGCAATTAAACGAGTTTCACC
ATGTGAGAAATTACATTCACAGGTATATTTTGCAGTGGCTGAAGCAACAA
CAAATAATAATAAACCTAAAAT
Gap no gap
Contig length 922
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 2329560
End point 2328634
Strand (PLUS/MINUS) MINUS
Number of clones 44
Number of EST 24
Link to clone list U10099
List of clone(s) 

est1=VSF490E,4,923
est2=VSF485E,11,923
est3=FC-IC1643E,64,269
est4=FC-IC1660E,64,269
est5=FC-IC0322E,65,269
est6=FC-IC0339E,65,269
est7=FC-IC0470E,65,269
est8=FC-IC0930E,65,269
est9=FC-IC1479E,65,269
est10=FC-IC0773F,271,751
est11=FC-IC0288E,287,751
est12=FC-IC0302Z,289,751
est13=FC-IC0921E,289,751
est14=FC-IC0951E,289,751
est15=FC-IC0952Z,289,751
est16=FC-IC1036E,289,751
est17=FC-IC1329E,289,751
est18=FC-IC1339F,289,751
est19=FC-IC1339Z,289,751
est20=FC-IC1365E,289,751
est21=FC-IC1612E,289,751
est22=FC-IC1629E,289,751
est23=FC-IC1694E,289,752
est24=FC-IC0162F,393,751
Translated Amino Acid sequence 
*sii*fiytltlkvlcymdskyhsiyql*LPCHYLNRYHHHFQNCHHFQNYHRYFHYFHC
FHYFHCYHLKQLIDQFGHQFDLRFDLRFGYLIVNLIVVLIVNLIVNLFVVLIVNLIANLF
VVLIVNPMFVNLMFVNLRFGNLKFGCLKMKFYQLMMDQDDEALPVDDGPEDEALPVDDGP
L**sstsc*wt***sstsc*wt***sstgllmgpr**gftscc*tlr*lmlllelvdnrk
*fhlyiykckfvnhme*yklle*wnyifpyneiyllqqlnefhhvrnyihryilqwlkqq
qiiinlk
Translated Amino Acid sequence (All Frames) 
Frame A:
*sii*fiytltlkvlcymdskyhsiyql*LPCHYLNRYHHHFQNCHHFQNYHRYFHYFHC
FHYFHCYHLKQLIDQFGHQFDLRFDLRFGYLIVNLIVVLIVNLIVNLFVVLIVNLIANLF
VVLIVNPMFVNLMFVNLRFGNLKFGCLKMKFYQLMMDQDDEALPVDDGPEDEALPVDDGP
L**sstsc*wt***sstsc*wt***sstgllmgpr**gftscc*tlr*lmlllelvdnrk
*fhlyiykckfvnhme*yklle*wnyifpyneiyllqqlnefhhvrnyihryilqwlkqq
qiiinlk


Frame B:
nr*fnlfil*hlkffvtwtvstiafinfnchatt*tattttsrtattsrtttatsttsta
sttstatt*ss*ltslgtslt*glt*glgt*l*t*lls*l*t*l*tclls*l*t*lrtcl
ls*l*trcl*t*cl*t*glgt*slga*r*sfts**wtkmmklyqlmmdlkmklyqlmmdh
ydealpvvdgpddealpvvdgpddealpgc*wdqddealpvvver*gs*cccwswltten
ssictftsasl*itwnsinflnngitsfhtmrfifysn*tsftm*eitftgifcsg*snn
k***t*n


Frame C:
idnliylysnt*ssllhgq*vp*hlstltamplpeplppplpelpplpelppllpllpll
pllpllpleavd*pvwapv*pev*pevwvpdcepdccpdcepdcelvccpdcepdcepvc
cpdcepdvcepdvcepevwepevwvledevlpvddgpr**sfts**wt*r*sfts**wtt
mmklyqllmdlmmklyqllmdlmmklyrvvdgtkmmrlyqlllnaevvdvvvgvg*qpki
vpfvhlqvqvckshgiv*ts*imelhlsiq*dlsftaikrvspceklhsqvyfavaeatt
nnnkpk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U10099-1 (Contig-U10099-1Q)
/CSM_Contig/Contig-U10099-1Q.Seq.d
         (922 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U10099-1 (Contig-U10099-1Q) /CSM_Contig/Conti...  1828  0.0
Contig-U14739-1 (Contig-U14739-1Q) /CSM_Contig/Conti...    44  3e-04
Contig-U12014-1 (Contig-U12014-1Q) /CSM_Contig/Conti...    44  3e-04
Contig-U14745-1 (Contig-U14745-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U11835-1 (Contig-U11835-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U10089-1 (Contig-U10089-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U11177-1 (Contig-U11177-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U14885-1 (Contig-U14885-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U12521-1 (Contig-U12521-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U11910-1 (Contig-U11910-1Q) /CSM_Contig/Conti...    38  0.016

>Contig-U10099-1 (Contig-U10099-1Q) /CSM_Contig/Contig-U10099-1Q.Seq.d
          Length = 922

 Score = 1828 bits (922), Expect = 0.0
 Identities = 922/922 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   taatcgataatttaatttatttatactctaacacttaaagttctttgttacatggacagt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   taatcgataatttaatttatttatactctaacacttaaagttctttgttacatggacagt 60

                                                                       
Query: 61  aagtaccatagcatttatcaactttaactgccatgccactacctgaaccgctaccaccac 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aagtaccatagcatttatcaactttaactgccatgccactacctgaaccgctaccaccac 120

                                                                       
Query: 121 cacttccagaactgccaccacttccagaactaccaccgctacttccactacttccactgc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 cacttccagaactgccaccacttccagaactaccaccgctacttccactacttccactgc 180

                                                                       
Query: 181 ttccactacttccactgctaccacttgaagcagttgattgaccagtttgggcaccagttt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttccactacttccactgctaccacttgaagcagttgattgaccagtttgggcaccagttt 240

                                                                       
Query: 241 gacctgaggtttgacctgaggtttgggtacctgattgtgaacctgattgttgtcctgatt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 gacctgaggtttgacctgaggtttgggtacctgattgtgaacctgattgttgtcctgatt 300

                                                                       
Query: 301 gtgaacctgattgtgaacttgtttgttgtcctgattgtgaacctgattgcgaacctgttt 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 gtgaacctgattgtgaacttgtttgttgtcctgattgtgaacctgattgcgaacctgttt 360

                                                                       
Query: 361 gttgtcctgattgtgaacccgatgtttgtgaacctgatgtttgtgaacctgaggtttggg 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 gttgtcctgattgtgaacccgatgtttgtgaacctgatgtttgtgaacctgaggtttggg 420

                                                                       
Query: 421 aacctgaagtttgggtgcttgaagatgaagttttaccagttgatgatggaccaagatgat 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 aacctgaagtttgggtgcttgaagatgaagttttaccagttgatgatggaccaagatgat 480

                                                                       
Query: 481 gaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgatggacca 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 gaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgatggacca 540

                                                                       
Query: 541 ctatgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttga 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 ctatgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttga 600

                                                                       
Query: 601 tggacctgatgatgaagctctaccgggttgttgatgggaccaagatgatgaggctttacc 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 tggacctgatgatgaagctctaccgggttgttgatgggaccaagatgatgaggctttacc 660

                                                                       
Query: 661 agttgttgttgaacgctgaggtagttgatgttgttgttggagttggttgacaaccgaaaa 720
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661 agttgttgttgaacgctgaggtagttgatgttgttgttggagttggttgacaaccgaaaa 720

                                                                       
Query: 721 tagttccatttgtacatttacaagtgcaagtttgtaaatcacatggaatagtataaactt 780
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 721 tagttccatttgtacatttacaagtgcaagtttgtaaatcacatggaatagtataaactt 780

                                                                       
Query: 781 cttgaataatggaattacatctttccatacaatgagatttatcttttacagcaattaaac 840
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781 cttgaataatggaattacatctttccatacaatgagatttatcttttacagcaattaaac 840

                                                                       
Query: 841 gagtttcaccatgtgagaaattacattcacaggtatattttgcagtggctgaagcaacaa 900
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 841 gagtttcaccatgtgagaaattacattcacaggtatattttgcagtggctgaagcaacaa 900

                                 
Query: 901 caaataataataaacctaaaat 922
           ||||||||||||||||||||||
Sbjct: 901 caaataataataaacctaaaat 922


>Contig-U14739-1 (Contig-U14739-1Q) /CSM_Contig/Contig-U14739-1Q.Seq.d
          Length = 1871

 Score = 44.1 bits (22), Expect = 3e-04
 Identities = 28/30 (93%)
 Strand = Plus / Minus

                                          
Query: 236  agtttgacctgaggtttgacctgaggtttg 265
            ||||||||| |||||||||||||| |||||
Sbjct: 1684 agtttgaccagaggtttgacctgaagtttg 1655


 Score = 32.2 bits (16), Expect = 1.0
 Identities = 19/20 (95%)
 Strand = Plus / Minus

                                
Query: 237  gtttgacctgaggtttgacc 256
            ||||||||||| ||||||||
Sbjct: 1671 gtttgacctgaagtttgacc 1652


 Score = 30.2 bits (15), Expect = 4.0
 Identities = 15/15 (100%)
 Strand = Plus / Minus

                           
Query: 211  cagttgattgaccag 225
            |||||||||||||||
Sbjct: 1595 cagttgattgaccag 1581


 Score = 30.2 bits (15), Expect = 4.0
 Identities = 15/15 (100%)
 Strand = Plus / Minus

                           
Query: 211  cagttgattgaccag 225
            |||||||||||||||
Sbjct: 1622 cagttgattgaccag 1608


 Score = 30.2 bits (15), Expect = 4.0
 Identities = 15/15 (100%)
 Strand = Plus / Minus

                           
Query: 111  ctaccaccaccactt 125
            |||||||||||||||
Sbjct: 1140 ctaccaccaccactt 1126


 Score = 30.2 bits (15), Expect = 4.0
 Identities = 15/15 (100%)
 Strand = Plus / Minus

                           
Query: 420  gaacctgaagtttgg 434
            |||||||||||||||
Sbjct: 1509 gaacctgaagtttgg 1495


>Contig-U12014-1 (Contig-U12014-1Q) /CSM_Contig/Contig-U12014-1Q.Seq.d
          Length = 1817

 Score = 44.1 bits (22), Expect = 3e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                      
Query: 682  tagttgatgttgttgttggagttggt 707
            ||||||||| ||||||||||||||||
Sbjct: 1430 tagttgatgatgttgttggagttggt 1455


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 29,613
Number of Sequences: 6905
Number of extensions: 29613
Number of successful extensions: 2704
Number of sequences better than 10.0: 356
length of query: 922
length of database: 5,674,871
effective HSP length: 16
effective length of query: 906
effective length of database: 5,564,391
effective search space: 5041338246
effective search space used: 5041338246
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.18
Homology vs DNA 
Query= Contig-U10099-1 (Contig-U10099-1Q) /CSM_Contig/Contig-U10099-1Q.Seq.d
         (922 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU265308) Dictyostelium discoideum vegetative cDNA clone:VS...   492   0.0    5
(AU265316) Dictyostelium discoideum vegetative cDNA clone:VS...   492   0.0    5
(AU265317) Dictyostelium discoideum vegetative cDNA clone:VS...   246   0.0    6
(AU265309) Dictyostelium discoideum vegetative cDNA clone:VS...   246   0.0    6
(AU271815) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   0.0    5
(AU272000) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   313   0.0    4
(AU275198) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU275199) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU275139) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU275138) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272440) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272439) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272423) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272422) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272415) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272075) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU272074) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-180  5
(AU271999) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   e-180  5
(AU271998) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   e-180  5
(AU275127) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   244   e-179  5
(AU275126) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   244   e-179  5
(AU272414) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   246   e-179  5
(AU271974) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   e-178  5
(AU271973) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   e-178  5
(AU271484) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   246   e-174  5
(AU271495) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   238   e-174  5
(AU271384) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   240   e-128  3
(AU275151) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   123   2e-44  2
(AU275152) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   123   6e-44  2
(AU272526) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   123   6e-44  2
(AU272525) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   123   6e-44  2
(AU271978) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271977) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271622) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271621) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271529) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271528) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU271515) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   123   6e-44  2
(AU275172) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   123   4e-42  2
(AU275171) Dictyostelium discoideum gamete cDNA clone:FC-IC1...    94   5e-35  2
(AU271485) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   153   1e-32  1
(BJ433456) Dictyostelium discoideum cDNA clone:ddv21n24, 3' ...    40   9e-10  5
(BJ431231) Dictyostelium discoideum cDNA clone:ddv13h07, 3' ...    40   1e-09  5
(BJ429984) Dictyostelium discoideum cDNA clone:ddv5k17, 3' e...    40   1e-09  5
(BJ428363) Dictyostelium discoideum cDNA clone:ddv11m15, 3' ...    40   1e-09  5
(BJ431755) Dictyostelium discoideum cDNA clone:ddv15m12, 3' ...    40   1e-09  5
(BJ428152) Dictyostelium discoideum cDNA clone:ddv10n19, 3' ...    40   2e-09  5
(BJ436003) Dictyostelium discoideum cDNA clone:ddv29m07, 3' ...    40   2e-09  5
(BJ435961) Dictyostelium discoideum cDNA clone:ddv29l01, 3' ...    40   2e-09  5
(BJ428922) Dictyostelium discoideum cDNA clone:ddv1a20, 3' e...    40   2e-09  5
(BJ431760) Dictyostelium discoideum cDNA clone:ddv15p07, 3' ...    40   2e-09  5
(BJ431878) Dictyostelium discoideum cDNA clone:ddv15l20, 3' ...    40   2e-09  5
(BJ431322) Dictyostelium discoideum cDNA clone:ddv13b20, 3' ...    40   3e-09  5
(BJ428495) Dictyostelium discoideum cDNA clone:ddv12m03, 3' ...    40   3e-09  5
(BJ442546) Dictyostelium discoideum cDNA clone:ddv50a09, 3' ...    40   3e-09  5
(AU039848) Dictyostelium discoideum slug cDNA, clone SLG572.       40   3e-09  5
(BJ431144) Dictyostelium discoideum cDNA clone:ddv13c03, 3' ...    40   3e-09  5
(BJ434983) Dictyostelium discoideum cDNA clone:ddv25m16, 3' ...    40   3e-09  5
(BJ436120) Dictyostelium discoideum cDNA clone:ddv30d05, 3' ...    40   3e-09  5
(BJ436196) Dictyostelium discoideum cDNA clone:ddv30h08, 3' ...    40   3e-09  5
(AU285037) Dictyostelium discoideum gamete cDNA clone:FCL-AB...    40   3e-09  5
(BJ433455) Dictyostelium discoideum cDNA clone:ddv21n23, 3' ...    40   3e-09  5
(BJ433057) Dictyostelium discoideum cDNA clone:ddv20l13, 3' ...    40   3e-09  5
(BJ428208) Dictyostelium discoideum cDNA clone:ddv11i06, 3' ...    40   3e-09  5
(BJ435330) Dictyostelium discoideum cDNA clone:ddv26e24, 3' ...    40   3e-09  5
(BJ434570) Dictyostelium discoideum cDNA clone:ddv24d07, 3' ...    40   3e-09  5
(BJ429237) Dictyostelium discoideum cDNA clone:ddv2e23, 3' e...    40   3e-09  5
(BJ433009) Dictyostelium discoideum cDNA clone:ddv20b16, 3' ...    40   3e-09  5
(BJ435755) Dictyostelium discoideum cDNA clone:ddv28p04, 3' ...    40   3e-09  5
(BJ429534) Dictyostelium discoideum cDNA clone:ddv3i24, 3' e...    40   3e-09  5
(BJ427936) Dictyostelium discoideum cDNA clone:ddv10j05, 3' ...    40   3e-09  5
(BJ431441) Dictyostelium discoideum cDNA clone:ddv14n05, 3' ...    40   3e-09  5
(BJ430071) Dictyostelium discoideum cDNA clone:ddv6a04, 3' e...    40   3e-09  5
(BJ429665) Dictyostelium discoideum cDNA clone:ddv4p10, 3' e...    40   3e-09  5
(BJ434909) Dictyostelium discoideum cDNA clone:ddv25l08, 3' ...    40   3e-09  5
(BJ433227) Dictyostelium discoideum cDNA clone:ddv21l02, 3' ...    40   3e-09  5
(BJ436016) Dictyostelium discoideum cDNA clone:ddv29p10, 3' ...    40   3e-09  5
(BJ435304) Dictyostelium discoideum cDNA clone:ddv26p18, 3' ...    40   3e-09  5
(BJ434728) Dictyostelium discoideum cDNA clone:ddv24d23, 3' ...    40   3e-09  5
(BJ433549) Dictyostelium discoideum cDNA clone:ddv22a09, 3' ...    40   3e-09  5
(BJ434373) Dictyostelium discoideum cDNA clone:ddv16n15, 3' ...    40   4e-09  5
(BJ433524) Dictyostelium discoideum cDNA clone:ddv22l02, 3' ...    40   4e-09  5
(BJ433491) Dictyostelium discoideum cDNA clone:ddv22e06, 3' ...    40   4e-09  5
(BJ435177) Dictyostelium discoideum cDNA clone:ddv26g11, 3' ...    40   4e-09  5
(BJ430772) Dictyostelium discoideum cDNA clone:ddv8n14, 3' e...    40   4e-09  5
(AU284347) Dictyostelium discoideum gamete cDNA clone:FC-AN1...    40   4e-09  5
(BJ431260) Dictyostelium discoideum cDNA clone:ddv13b16, 3' ...    40   4e-09  5
(BJ435670) Dictyostelium discoideum cDNA clone:ddv27h23, 3' ...    40   4e-09  5
(BJ434215) Dictyostelium discoideum cDNA clone:ddv16p05, 3' ...    40   4e-09  5
(BJ435977) Dictyostelium discoideum cDNA clone:ddv29p04, 3' ...    40   4e-09  5
(BJ434343) Dictyostelium discoideum cDNA clone:ddv16h17, 3' ...    40   4e-09  5
(BJ432695) Dictyostelium discoideum cDNA clone:ddv19p08, 3' ...    40   4e-09  5
(AU034293) Dictyostelium discoideum slug cDNA, clone SLC532.       36   4e-09  5
(BJ430070) Dictyostelium discoideum cDNA clone:ddv6a03, 3' e...    40   4e-09  5
(C93148) Dictyostelium discoideum slug cDNA, clone SSM682.         40   4e-09  5
(BJ434046) Dictyostelium discoideum cDNA clone:ddv23a24, 3' ...    40   4e-09  5
(BJ428936) Dictyostelium discoideum cDNA clone:ddv1c23, 3' e...    40   4e-09  5
(BJ428258) Dictyostelium discoideum cDNA clone:ddv11e09, 3' ...    40   4e-09  5
(BJ434246) Dictyostelium discoideum cDNA clone:ddv16f08, 3' ...    40   4e-09  5
(BJ430169) Dictyostelium discoideum cDNA clone:ddv6f10, 3' e...    40   4e-09  5

>(AU265308) Dictyostelium discoideum vegetative cDNA clone:VSF485,
           5' end single read.
          Length = 539

 Score =  492 bits (248), Expect(5) = 0.0
 Identities = 248/248 (100%)
 Strand = Plus / Minus


Query: 675 gctgaggtagttgatgttgttgttggagttggttgacaaccgaaaatagttccatttgta 734
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 248 gctgaggtagttgatgttgttgttggagttggttgacaaccgaaaatagttccatttgta 189


Query: 735 catttacaagtgcaagtttgtaaatcacatggaatagtataaacttcttgaataatggaa 794
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 188 catttacaagtgcaagtttgtaaatcacatggaatagtataaacttcttgaataatggaa 129


Query: 795 ttacatctttccatacaatgagatttatcttttacagcaattaaacgagtttcaccatgt 854
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 128 ttacatctttccatacaatgagatttatcttttacagcaattaaacgagtttcaccatgt 69


Query: 855 gagaaattacattcacaggtatattttgcagtggctgaagcaacaacaaataataataaa 914
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 68  gagaaattacattcacaggtatattttgcagtggctgaagcaacaacaaataataataaa 9


Query: 915 cctaaaat 922
           ||||||||
Sbjct: 8   cctaaaat 1

 Score =  190 bits (96), Expect(5) = 0.0
 Identities = 96/96 (100%)
 Strand = Plus / Minus


Query: 378 cccgatgtttgtgaacctgatgtttgtgaacctgaggtttgggaacctgaagtttgggtg 437
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 539 cccgatgtttgtgaacctgatgtttgtgaacctgaggtttgggaacctgaagtttgggtg 480


Query: 438 cttgaagatgaagttttaccagttgatgatggacca 473
           ||||||||||||||||||||||||||||||||||||
Sbjct: 479 cttgaagatgaagttttaccagttgatgatggacca 444

 Score =  167 bits (84), Expect(5) = 0.0
 Identities = 84/84 (100%)
 Strand = Plus / Minus


Query: 543 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatg 602
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 376 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatg 317


Query: 603 gacctgatgatgaagctctaccgg 626
           ||||||||||||||||||||||||
Sbjct: 316 gacctgatgatgaagctctaccgg 293

 Score =  133 bits (67), Expect(5) = 0.0
 Identities = 67/67 (100%)
 Strand = Plus / Minus


Query: 474 agatgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatga 533
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 444 agatgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatga 385


Query: 534 tggacca 540
           |||||||
Sbjct: 384 tggacca 378

 Score = 61.9 bits (31), Expect(5) = 0.0
 Identities = 31/31 (100%)
 Strand = Plus / Minus


Query: 643 agatgatgaggctttaccagttgttgttgaa 673
           |||||||||||||||||||||||||||||||
Sbjct: 279 agatgatgaggctttaccagttgttgttgaa 249

 Score =  131 bits (66), Expect(4) = 1e-67
 Identities = 78/82 (95%)
 Strand = Plus / Minus


Query: 543 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatg 602
           ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 343 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccggttgttgatg 284


Query: 603 gacctgatgatgaagctctacc 624
           |||| |||||||| ||| ||||
Sbjct: 283 gaccagatgatgaggctttacc 262

 Score = 91.7 bits (46), Expect(4) = 1e-67
 Identities = 58/62 (93%)
 Strand = Plus / Minus


Query: 478 gatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgatgga 537
           ||||||||||||||||||||||||||||| || ||||||||| ||||||||| |||||||
Sbjct: 407 gatgaagctttaccagttgatgatggaccagatgatgaagctctaccagttgttgatgga 348


Query: 538 cc 539
           ||
Sbjct: 347 cc 346

 Score = 67.9 bits (34), Expect(4) = 1e-67
 Identities = 46/50 (92%)
 Strand = Plus / Minus


Query: 371 ttgtgaacccgatgtttgtgaacctgatgtttgtgaacctgaggtttggg 420
           ||||||||| ||||||||||||||||| ||||| |||||||| |||||||
Sbjct: 531 ttgtgaacctgatgtttgtgaacctgaggtttgggaacctgaagtttggg 482

 Score = 50.1 bits (25), Expect(4) = 1e-67
 Identities = 28/29 (96%)
 Strand = Plus / Minus


Query: 444 gatgaagttttaccagttgatgatggacc 472
           ||||||| |||||||||||||||||||||
Sbjct: 440 gatgaagctttaccagttgatgatggacc 412

 Score =  135 bits (68), Expect(3) = 3e-59
 Identities = 77/80 (96%)
 Strand = Plus / Minus


Query: 545 gatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatgga 604
           ||||||||| ||||||||| ||||||||| ||||||||||||||||||||||||||||||
Sbjct: 407 gatgaagctttaccagttgatgatggaccagatgatgaagctctaccagttgttgatgga 348


Query: 605 cctgatgatgaagctctacc 624
           ||||||||||||||||||||
Sbjct: 347 cctgatgatgaagctctacc 328

 Score = 99.6 bits (50), Expect(3) = 3e-59
 Identities = 59/62 (95%)
 Strand = Plus / Minus


Query: 478 gatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgatgga 537
           ||||||| ||||||||||||||||||||| || |||||||||||||||||||||||||||
Sbjct: 473 gatgaagttttaccagttgatgatggaccagatgatgaagctttaccagttgatgatgga 414


Query: 538 cc 539
           ||
Sbjct: 413 cc 412

 Score = 56.0 bits (28), Expect(3) = 3e-59
 Identities = 37/40 (92%)
 Strand = Plus / Minus


Query: 396 gatgtttgtgaacctgaggtttgggaacctgaagtttggg 435
           ||||||||||||||||| ||||| |||||||| |||||||
Sbjct: 536 gatgtttgtgaacctgatgtttgtgaacctgaggtttggg 497

 Score = 91.7 bits (46), Expect(3) = 4e-45
 Identities = 61/66 (92%)
 Strand = Plus / Minus


Query: 474 agatgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatga 533
           ||||||||||||| ||||||||| |||||||||||| ||||||||| ||||||||| |||
Sbjct: 378 agatgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttga 319


Query: 534 tggacc 539
           ||||||
Sbjct: 318 tggacc 313

 Score = 81.8 bits (41), Expect(3) = 4e-45
 Identities = 53/57 (92%)
 Strand = Plus / Minus


Query: 543 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttg 599
           |||||||||||||||| |||||||||||||| |||||||| ||| ||||||||||||
Sbjct: 310 atgatgaagctctaccggttgttgatggaccagatgatgaggctttaccagttgttg 254

 Score = 60.0 bits (30), Expect(3) = 4e-45
 Identities = 33/34 (97%)
 Strand = Plus / Minus


Query: 440 tgaagatgaagttttaccagttgatgatggacca 473
           ||||||||||| ||||||||||||||||||||||
Sbjct: 411 tgaagatgaagctttaccagttgatgatggacca 378

 Score =  115 bits (58), Expect(3) = 5e-38
 Identities = 76/82 (92%)
 Strand = Plus / Minus


Query: 543 atgatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatg 602
           ||||||||||| ||||||||| |||||||||||| ||||||||| ||||||||| |||||
Sbjct: 442 atgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgatg 383


Query: 603 gacctgatgatgaagctctacc 624
           |||| |||||||||||||||||
Sbjct: 382 gaccagatgatgaagctctacc 361

 Score = 60.0 bits (30), Expect(3) = 5e-38
 Identities = 33/34 (97%)
 Strand = Plus / Minus


Query: 507 tgaagatgaagctttaccagttgatgatggacca 540
           ||||||||||| ||||||||||||||||||||||
Sbjct: 477 tgaagatgaagttttaccagttgatgatggacca 444

 Score = 34.2 bits (17), Expect(3) = 5e-38
 Identities = 23/25 (92%)
 Strand = Plus / Minus


Query: 644 gatgatgaggctttaccagttgttg 668
           |||||||| ||| ||||||||||||
Sbjct: 344 gatgatgaagctctaccagttgttg 320

 Score = 95.6 bits (48), Expect(3) = 1e-20
 Identities = 72/80 (90%)
 Strand = Plus / Minus


Query: 545 gatgaagctctaccagttgttgatggacctgatgatgaagctctaccagttgttgatgga 604
           ||||||| | ||||||||| ||||||||| |||||||||||| ||||||||| |||||||
Sbjct: 473 gatgaagttttaccagttgatgatggaccagatgatgaagctttaccagttgatgatgga 414


Query: 605 cctgatgatgaagctctacc 624
           ||||| ||||||||| ||||
Sbjct: 413 cctgaagatgaagctttacc 394

 Score = 36.2 bits (18), Expect(3) = 1e-20
 Identities = 24/26 (92%)
 Strand = Plus / Minus


Query: 643 agatgatgaggctttaccagttgttg 668
           ||||||||| ||| ||||||||||||
Sbjct: 378 agatgatgaagctctaccagttgttg 353

 Score = 28.2 bits (14), Expect(3) = 1e-20
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 254 acctgaggtttggg 267
           ||||||||||||||
Sbjct: 510 acctgaggtttggg 497

 Score = 83.8 bits (42), Expect(2) = 1e-15
 Identities = 60/66 (90%)
 Strand = Plus / Minus


Query: 475 gatgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttgatgat 534
           |||||||||||| ||||||||| |||||||||||| ||||||||| |||| |||| ||||
Sbjct: 344 gatgatgaagctctaccagttgttgatggacctgatgatgaagctctaccggttgttgat 285


Query: 535 ggacca 540
           ||||||
Sbjct: 284 ggacca 279

 Score = 32.2 bits (16), Expect(2) = 1e-15
 Identities = 22/24 (91%)
 Strand = Plus / Minus


Query: 543 atgatgaagctctaccagttgttg 566
           ||||||| ||| ||||||||||||
Sbjct: 277 atgatgaggctttaccagttgttg 254

 Score = 54.0 bits (27), Expect(2) = 3e-06
 Identities = 42/47 (89%)
 Strand = Plus / Minus


Query: 578 gatgaagctctaccagttgttgatggacctgatgatgaagctctacc 624
           ||||||| | ||||||||| ||||||||| |||||||||||| ||||
Sbjct: 473 gatgaagttttaccagttgatgatggaccagatgatgaagctttacc 427

 Score = 30.2 bits (15), Expect(2) = 3e-06
 Identities = 18/19 (94%)
 Strand = Plus / Minus


Query: 647 gatgaggctttaccagttg 665
           ||||| |||||||||||||
Sbjct: 407 gatgaagctttaccagttg 389

 Score = 61.9 bits (31), Expect = 4e-05
 Identities = 49/55 (89%)
 Strand = Plus / Minus


Query: 475 gatgatgaagctttaccagttgatgatggacctgaagatgaagctttaccagttg 529
           |||||||||||| |||| |||| ||||||||| || ||||| |||||||||||||
Sbjct: 311 gatgatgaagctctaccggttgttgatggaccagatgatgaggctttaccagttg 257

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 840,760,710
Number of extensions: 56331646
Number of successful extensions: 4269372
Number of sequences better than 10.0: 248
Length of query: 922
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 898
Effective length of database: 97,308,875,965
Effective search space: 87383370616570
Effective search space used: 87383370616570
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.27
Homology vs Protein 
Query= Contig-U10099-1 (Contig-U10099-1Q) /CSM_Contig/Contig-U10099-1Q.Seq.d
         (922 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PA8) RecName: Full=Uncharacterized protein DDB_G0284671; Fla...   157   2e-49
CP000850_4884(CP000850|pid:none) Salinispora arenicola CNS-205, ...    38   0.55
AM502236_87(AM502236|pid:none) Leishmania infantum chromosome 18.      35   4.6
BC044075_1(BC044075|pid:none) Xenopus laevis unactive progestero...    35   4.6
AE016853_4002(AE016853|pid:none) Pseudomonas syringae pv. tomato...    34   7.9
A82704(A82704) 1,4-beta-cellobiosidase XF1267 [imported] - Xylel...    34   7.9
EF452764_1(EF452764|pid:none) Danio kyathit NADH dehydrogenase s...    34   7.9

>(Q54PA8) RecName: Full=Uncharacterized protein DDB_G0284671; Flags:
           Precursor;
          Length = 305

 Score =  157 bits (396), Expect(2) = 2e-49
 Identities = 72/72 (100%), Positives = 72/72 (100%)
 Frame = -1

Query: 922 ILGLLLFVVASATAKYTCECNFSHGETRLIAVKDKSHCMERCNSIIQEVYTIPCDLQTCT 743
           ILGLLLFVVASATAKYTCECNFSHGETRLIAVKDKSHCMERCNSIIQEVYTIPCDLQTCT
Sbjct: 7   ILGLLLFVVASATAKYTCECNFSHGETRLIAVKDKSHCMERCNSIIQEVYTIPCDLQTCT 66

Query: 742 CKCTNGTIFGCQ 707
           CKCTNGTIFGCQ
Sbjct: 67  CKCTNGTIFGCQ 78

 Score = 63.5 bits (153), Expect(2) = 2e-49
 Identities = 35/41 (85%), Positives = 35/41 (85%)
 Frame = -2

Query: 672 STTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTGRA 550
           STTTGKASSS      GRASSSGPSTTGRASSSGPSTTGRA
Sbjct: 90  STTTGKASSS-GPSTTGRASSSGPSTTGRASSSGPSTTGRA 129

 Score = 56.6 bits (135), Expect = 1e-06
 Identities = 30/40 (75%), Positives = 34/40 (85%)
 Frame = -2

Query: 669 TTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTGRA 550
           +TTG+ASSS      GRASSSGPSTTGRASSSGPS+TG+A
Sbjct: 102 STTGRASSS-GPSTTGRASSSGPSTTGRASSSGPSSTGKA 140

 Score = 53.9 bits (128), Expect = 7e-06
 Identities = 28/40 (70%), Positives = 34/40 (85%)
 Frame = -2

Query: 669 TTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTGRA 550
           +TTG+ASSS      GRASSSGPS+TG+ASSSGPS+TG+A
Sbjct: 113 STTGRASSS-GPSTTGRASSSGPSSTGKASSSGPSSTGKA 151

 Score = 53.5 bits (127), Expect = 1e-05
 Identities = 29/40 (72%), Positives = 32/40 (80%)
 Frame = -2

Query: 669 TTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTGRA 550
           TTT   S+S +    G+ASSSGPSTTGRASSSGPSTTGRA
Sbjct: 82  TTTSTTSASTT---TGKASSSGPSTTGRASSSGPSTTGRA 118

 Score = 51.6 bits (122), Expect = 4e-05
 Identities = 26/39 (66%), Positives = 33/39 (84%)
 Frame = -2

Query: 669 TTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTGR 553
           +TTG+ASSS      G+ASSSGPS+TG+ASSSGPS+TG+
Sbjct: 124 STTGRASSS-GPSSTGKASSSGPSSTGKASSSGPSSTGK 161

 Score = 47.8 bits (112), Expect = 5e-04
 Identities = 19/19 (100%), Positives = 19/19 (100%)
 Frame = -1

Query: 94  MAVKVDKCYGTYCPCNKEL 38
           MAVKVDKCYGTYCPCNKEL
Sbjct: 281 MAVKVDKCYGTYCPCNKEL 299

 Score = 42.0 bits (97), Expect = 0.029
 Identities = 22/38 (57%), Positives = 28/38 (73%)
 Frame = -2

Query: 669 TTTGKASSSWSHQQPGRASSSGPSTTGRASSSGPSTTG 556
           ++TGKASSS      G+ASSSGPS+TG+ SSS   T+G
Sbjct: 135 SSTGKASSS-GPSSTGKASSSGPSSTGKTSSSSTQTSG 171

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 950,904,949
Number of extensions: 16197980
Number of successful extensions: 59817
Number of sequences better than 10.0: 7
Number of HSP's gapped: 56563
Number of HSP's successfully gapped: 14
Length of query: 307
Length of database: 1,040,966,779
Length adjustment: 128
Effective length of query: 179
Effective length of database: 630,818,299
Effective search space: 112916475521
Effective search space used: 112916475521
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.67  gvh: 0.23  alm: 0.10  top: 0.83  tms: 0.13  mit: 0.39  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 24.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 16.0 %: vacuolar
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for Contig-U10099-1 is pla
VS (DIR, S) 2
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 21
FC-IC (SUB) 21