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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U01623-1
Contig ID Contig-U01623-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U01623-1 (Contig-U01623-1Q) /CSM_Contig/Contig-U01623-1Q.Seq.d
GATTTAACGACCATTTATATTTTGGGTATTACATTTAAAAATGGTAACCA
ATCAAGTGGTTCAGTAATTTTAAATCAAGGTAGTTGGGTAGATATTACAA
TCGATCAATCATCATTTATTAATAATGGTGCAAGTCAAGTTGGTGGTAGT
TTTGCAATGATTAATGGTATTGGTAATAGTGGTGGTGGTTCTGGTGCAAT
TAATAGTACTTTAACTATAATTAATTCAAGTTTTATTAATACAACCACAA
CAACAACAACAACAATATTAAATAATAATAATCAAAATCAATCAAATGAA
ATTAAAAAAGAATTAACAACAATTGGTGGTATAATTTTTTCAAATTCAAC
AACACTAATAACAATTAATGATTGTAGATTTTTTAATAATAGTGGTAATA
ATGGTATTGGTTATATTTGGTCAGGTTATTTAGTGATGAATAATTCATTG
ATTGAAGGTAGTAATTGTTCACCAGCATTCTTTTTCAATTCAATAAGTGG
TATGGGTACAGGTTCACCATTGTTCTTGATATCAAATACAACATTTACAA
ATGCAATCGGTGGTGTAGGGTTTGTTGTTTTTGGTTCTGATTCAACTACA
ACCTTTCAACAATGTACCTTTAGTGATAATGTTAATACCATACCATTATC
TCTAAGTAATT----------TTTCAAATAATAACAATAGTAAATCATTG
ACAGAGTCGGCAGGTGCAATTAATATCATTGATTCTTCAATTATAATAGA
GTCTTCCACATTCATCAATAATAGAGCAGCCATTGGTGGTGCAATTCAAA
TGAATGGTTCATTACCTGAACAGTATGTAAAAATCTATAATTCAGTATTT
AATGGTAATAATGCAACCGATATTGGTGGTTCTATTTTCTCTCAAGAGGG
TCAATTATATCTCTACAGTTGTCAATTCTTAAATAATGAAGCGGTTGCTG
GTTCATCAGTTTATTGTTTAAATTCAAATATAAACTTTAGTAATATGACA
TTTAACAATAATACCGATTCATCAATACCAACTCCAAATGGTATTGGTTG
TGGTTCTGGTAAAGTTTGTTCCATTCAAGGTGATCAACAATTTCAAAATA
GTTGTTCCTATGATTATCAACCTGATAAACCATCATTACTATCTCCTGGT
GAAGTGGCTGCAATCGTAATTTGTGTTATAATTGGTGCTGCTATCAATAC
AACTGTTTTGATTTTAATCATTCGTAAAATTAGTAGAGGAAGAAATGGTT
ATTCCAAAATTGTTAATGGTTAAACATTTACTACTCGACTTTTTATAAAA
AAAAAAAAAAAAAATTAAAAAAAATTATAATATTATATAAATATTTTATA
TGAAAAAGGTCTTTTTTTTTTCCATCACTAGATATTAAATAAATAAAAAA
AATATTAAAAAAAAAC
Gap gap included
Contig length 1406
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 5937080
End point 5935673
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 4
Link to clone list U01623
List of clone(s) 

est1=SSC874F,1,662
est2=SSC874Z,663,1307
est3=VSE381Z,676,1287
est4=SSB148Z,863,1408
Translated Amino Acid sequence 
DLTTIYILGITFKNGNQSSGSVILNQGSWVDITIDQSSFINNGASQVGGSFAMINGIGNS
GGGSGAINSTLTIINSSFINTTTTTTTTILNNNNQNQSNEIKKELTTIGGIIFSNSTTLI
TINDCRFFNNSGNNGIGYIWSGYLVMNNSLIEGSNCSPAFFFNSISGMGTGSPLFLISNT
TFTNAIGGVGFVVFGSDSTTTFQQCTFSDNVNTIPLSLSN---

---SNNNNSKSLTESAGAINIIDSSIIIESSTFINNRAAIGGAIQMNGSLPEQYVKIYNS
VFNGNNATDIGGSIFSQEGQLYLYSCQFLNNEAVAGSSVYCLNSNINFSNMTFNNNTDSS
IPTPNGIGCGSGKVCSIQGDQQFQNSCSYDYQPDKPSLLSPGEVAAIVICVIIGAAINTT
VLILIIRKISRGRNGYSKIVNG*tfttrlfikkkkkn*kkl*yyinilyekglfffhh*i
lnk*kky*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
DLTTIYILGITFKNGNQSSGSVILNQGSWVDITIDQSSFINNGASQVGGSFAMINGIGNS
GGGSGAINSTLTIINSSFINTTTTTTTTILNNNNQNQSNEIKKELTTIGGIIFSNSTTLI
TINDCRFFNNSGNNGIGYIWSGYLVMNNSLIEGSNCSPAFFFNSISGMGTGSPLFLISNT
TFTNAIGGVGFVVFGSDSTTTFQQCTFSDNVNTIPLSLSN---

---fqiitivnh*qsrqvqlislilql**slphssiieqplvvqfk*mvhylnsm*ksii
qylmvimqpilvvlfslkrvnyistvvns*imkrllvhqfiv*iqi*tlvi*hltiipih
qyqlqmvlvvvlvkfvpfkvinnfkivvpmiinlinhhyyllvkwlqs*fvl*lvllsiq
lf*f*sfvklveeemvipkllmvkhllldfl*kkkkkikknynii*ifymkkvfffsitr
y*inkknikkk

Frame B:
i*rpfifwvlhlkmvtnqvvq*f*ikvvg*ilqsinhhllimvqvklvvvlq*lmvlviv
vvvlvqlivl*l*liqvlliqpqqqqqqy*iiiikinqmklkkn*qqlvv*ffqiqqh**
qlmivdfliivvimvlvifgqvi***iih*lkvvivhqhsfsiq*vvwvqvhhcs*yqiq
hlqmqsvv*gllflvliqlqpfnnvplvimlipyhyl*vi---

---fk**q**iidrvgrcn*yh*ffnynrvfhihq**sshwwcnsnewfit*tvcknl*f
si*w**cnrywwfyflsrgsiislqlsilk**sgcwfisllfkfkykl**ydi*q*yrfi
ntnskwywlwfw*slfhsr*stisk*lfl*lst**tiitisw*sgcnrnlcynwccyqyn
cfdfnhs*n**rkkwlfqnc*wlniyystfykkkkkklkkiiilykyfi*krsfffpsld
ik*ikkilkkn

Frame C:
fndhlyfgyyi*kw*pikwfsnfksr*lgryynrsiiiy**wckssww*fcnd*wyw**w
wwfwcn**yfnyn*fkfy*ynhnnnnnnik***sksik*n*krinnnwwynffkfnntnn
n**l*if***w**wywlylvrlfsde*fid*r**lftsilfqfnkwygyrftivldikyn
iykcnrwcrvccfwf*fnynlstmyl***c*yhtiisk*---

---SNNNNSKSLTESAGAINIIDSSIIIESSTFINNRAAIGGAIQMNGSLPEQYVKIYNS
VFNGNNATDIGGSIFSQEGQLYLYSCQFLNNEAVAGSSVYCLNSNINFSNMTFNNNTDSS
IPTPNGIGCGSGKVCSIQGDQQFQNSCSYDYQPDKPSLLSPGEVAAIVICVIIGAAINTT
VLILIIRKISRGRNGYSKIVNG*tfttrlfikkkkkn*kkl*yyinilyekglfffhh*i
lnk*kky*kk
own update 2004. 6. 7
Homology vs CSM-cDNA 
Query= Contig-U01623-1 (Contig-U01623-1Q)
/CSM_Contig/Contig-U01623-1Q.Seq.d
         (1416 letters)

Database: CSM
           8361 sequences; 7,895,291 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U01623-1 (Contig-U01623-1Q) /CSM_Contig/Conti...  1883  0.0
Contig-U00712-1 (Contig-U00712-1Q) /CSM_Contig/Conti...    44  6e-04
Contig-U12540-1 (Contig-U12540-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U13643-1 (Contig-U13643-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U13206-1 (Contig-U13206-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U12453-1 (Contig-U12453-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U12199-1 (Contig-U12199-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U11406-1 (Contig-U11406-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U07866-1 (Contig-U07866-1Q) /CSM_Contig/Conti...    40  0.009
Contig-U06349-1 (Contig-U06349-1Q) /CSM_Contig/Conti...    34  0.55

>Contig-U01623-1 (Contig-U01623-1Q) /CSM_Contig/Contig-U01623-1Q.Seq.d
          Length = 1416

 Score = 1883 bits (950), Expect = 0.0
 Identities = 980/980 (100%)
 Strand = Plus / Plus

                                                                        
Query: 317  caacaattggtggtataattttttcaaattcaacaacactaataacaattaatgattgta 376
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 317  caacaattggtggtataattttttcaaattcaacaacactaataacaattaatgattgta 376

                                                                        
Query: 377  gattttttaataatagtggtaataatggtattggttatatttggtcaggttatttagtga 436
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 377  gattttttaataatagtggtaataatggtattggttatatttggtcaggttatttagtga 436

                                                                        
Query: 437  tgaataattcattgattgaaggtagtaattgttcaccagcattctttttcaattcaataa 496
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 437  tgaataattcattgattgaaggtagtaattgttcaccagcattctttttcaattcaataa 496

                                                                        
Query: 497  gtggtatgggtacaggttcaccattgttcttgatatcaaatacaacatttacaaatgcaa 556
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 497  gtggtatgggtacaggttcaccattgttcttgatatcaaatacaacatttacaaatgcaa 556

                                                                        
Query: 557  tcggtggtgtagggtttgttgtttttggttctgattcaactacaacctttcaacaatgta 616
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 557  tcggtggtgtagggtttgttgtttttggttctgattcaactacaacctttcaacaatgta 616

                                                                        
Query: 617  cctttagtgataatgttaataccataccattatctctaagtaattnnnnnnnnnntttca 676
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 617  cctttagtgataatgttaataccataccattatctctaagtaattnnnnnnnnnntttca 676

                                                                        
Query: 677  aataataacaatagtaaatcattgacagagtcggcaggtgcaattaatatcattgattct 736
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 677  aataataacaatagtaaatcattgacagagtcggcaggtgcaattaatatcattgattct 736

                                                                        
Query: 737  tcaattataatagagtcttccacattcatcaataatagagcagccattggtggtgcaatt 796
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 737  tcaattataatagagtcttccacattcatcaataatagagcagccattggtggtgcaatt 796

                                                                        
Query: 797  caaatgaatggttcattacctgaacagtatgtaaaaatctataattcagtatttaatggt 856
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 797  caaatgaatggttcattacctgaacagtatgtaaaaatctataattcagtatttaatggt 856

                                                                        
Query: 857  aataatgcaaccgatattggtggttctattttctctcaagagggtcaattatatctctac 916
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 857  aataatgcaaccgatattggtggttctattttctctcaagagggtcaattatatctctac 916

                                                                        
Query: 917  agttgtcaattcttaaataatgaagcggttgctggttcatcagtttattgtttaaattca 976
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 917  agttgtcaattcttaaataatgaagcggttgctggttcatcagtttattgtttaaattca 976

                                                                        
Query: 977  aatataaactttagtaatatgacatttaacaataataccgattcatcaataccaactcca 1036
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 977  aatataaactttagtaatatgacatttaacaataataccgattcatcaataccaactcca 1036

                                                                        
Query: 1037 aatggtattggttgtggttctggtaaagtttgttccattcaaggtgatcaacaatttcaa 1096
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1037 aatggtattggttgtggttctggtaaagtttgttccattcaaggtgatcaacaatttcaa 1096

                                                                        
Query: 1097 aatagttgttcctatgattatcaacctgataaaccatcattactatctcctggtgaagtg 1156
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1097 aatagttgttcctatgattatcaacctgataaaccatcattactatctcctggtgaagtg 1156

                                                                        
Query: 1157 gctgcaatcgtaatttgtgttataattggtgctgctatcaatacaactgttttgatttta 1216
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1157 gctgcaatcgtaatttgtgttataattggtgctgctatcaatacaactgttttgatttta 1216

                                                                        
Query: 1217 atcattcgtaaaattagtagaggaagaaatggttattccaaaattgttaatggttaaaca 1276
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1217 atcattcgtaaaattagtagaggaagaaatggttattccaaaattgttaatggttaaaca 1276

                                
Query: 1277 tttactactcgactttttat 1296
            ||||||||||||||||||||
Sbjct: 1277 tttactactcgactttttat 1296


 Score =  476 bits (240), Expect = e-134
 Identities = 240/240 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   gatttaacgaccatttatattttgggtattacatttaaaaatggtaaccaatcaagtggt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   gatttaacgaccatttatattttgggtattacatttaaaaatggtaaccaatcaagtggt 60

                                                                       
Query: 61  tcagtaattttaaatcaaggtagttgggtagatattacaatcgatcaatcatcatttatt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tcagtaattttaaatcaaggtagttgggtagatattacaatcgatcaatcatcatttatt 120

                                                                       
Query: 121 aataatggtgcaagtcaagttggtggtagttttgcaatgattaatggtattggtaatagt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aataatggtgcaagtcaagttggtggtagttttgcaatgattaatggtattggtaatagt 180

                                                                       
Query: 181 ggtggtggttctggtgcaattaatagtactttaactataattaattcaagttttattaat 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ggtggtggttctggtgcaattaatagtactttaactataattaattcaagttttattaat 240


 Score = 73.8 bits (37), Expect = 6e-13
 Identities = 37/37 (100%)
 Strand = Plus / Plus

                                                 
Query: 1325 ttataatattatataaatattttatatgaaaaaggtc 1361
            |||||||||||||||||||||||||||||||||||||
Sbjct: 1325 ttataatattatataaatattttatatgaaaaaggtc 1361


>Contig-U00712-1 (Contig-U00712-1Q) /CSM_Contig/Contig-U00712-1Q.Seq.d
          Length = 1711

 Score = 44.1 bits (22), Expect = 6e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                  
Query: 677  aataataacaatagtaaatcat 698
            ||||||||||||||||||||||
Sbjct: 1099 aataataacaatagtaaatcat 1120


>Contig-U12540-1 (Contig-U12540-1Q) /CSM_Contig/Contig-U12540-1Q.Seq.d
          Length = 1281

 Score = 42.1 bits (21), Expect = 0.002
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 673 ttcaaataataacaatagtaa 693
           |||||||||||||||||||||
Sbjct: 922 ttcaaataataacaatagtaa 942


  Database: CSM
    Posted date:  Jun 7, 2004  8:33 AM
  Number of letters in database: 7,895,291
  Number of sequences in database:  8361
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 34,291
Number of Sequences: 8361
Number of extensions: 34291
Number of successful extensions: 4476
Number of sequences better than 10.0: 515
length of query: 1416
length of database: 7,895,291
effective HSP length: 16
effective length of query: 1400
effective length of database: 7,761,515
effective search space: 10866121000
effective search space used: 10866121000
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 5. 3
Homology vs DNA 
Query= Contig-U01623-1 (Contig-U01623-1Q) /CSM_Contig/Contig-U01623-1Q.Seq.d
         (1416 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C93972) Dictyostelium discoideum slug cDNA, clone SSC874.       1227   0.0    1
(AU263067) Dictyostelium discoideum vegetative cDNA clone:VS...  1197   0.0    1
(AU051840) Dictyostelium discoideum slug cDNA, clone SSB148.      729   0.0    4
(AU071973) Dictyostelium discoideum slug cDNA, clone SSC874.      476   e-129  1
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...    38   2e-04  14
(CP001184) Ureaplasma urealyticum serovar 10 str. ATCC 33699...    42   6e-04  20
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    36   0.001  16
(AC115605) Dictyostelium discoideum chromosome 2 map 5817431...    34   0.007  6
(CP000942) Ureaplasma parvum serovar 3 str. ATCC 27815, comp...    42   0.010  21
(AF222894) Ureaplasma parvum serovar 3 str. ATCC 700970, com...    42   0.010  21
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...    38   0.042  16
(AE014845) Plasmodium falciparum 3D7 chromosome 12, section ...    38   0.043  9
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    32   0.044  16
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    38   0.048  12
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    42   0.049  16
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...    38   0.099  11
(AC084846) Homo sapiens chromosome 11 clone CTD-2322L7 map 1...    36   0.22   8
(ET794646) CHO_OF658xa23r1.ab1 CHO_OF Nicotiana tabacum geno...    50   0.24   1
(ET794590) CHO_OF658xa23f1.ab1 CHO_OF Nicotiana tabacum geno...    50   0.24   1
(FD860154) CBHA8460.fwd CBHA Volvox carteri f. nagariensis i...    50   0.24   1
(FD860153) CBHA8460.rev CBHA Volvox carteri f. nagariensis i...    50   0.24   1
(CP000551) Prochlorococcus marinus str. AS9601, complete gen...    50   0.24   1
(AL844509) Plasmodium falciparum chromosome 13.                    38   0.31   13
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    38   0.36   16
(AE002104) Ureaplasma parvum serovar 3 str. ATCC 700970 sect...    42   0.37   6
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    34   0.41   14
(EF710659) Glyptapanteles indiensis clone BAC 9I20, complete...    40   0.51   8
(AC024197) Homo sapiens chromosome 8 clone RP11-711G22, WORK...    42   0.62   5
(GE722881) CBUP3718.b1 B.anynana_wing.4-6d_2-10kb Bicyclus a...    36   0.67   2
(AC115684) Dictyostelium discoideum chromosome 2 map 3108975...    34   0.79   9
(AC116989) Dictyostelium discoideum chromosome 2 map 4545040...    36   0.82   6
(AC121245) Medicago truncatula clone mth2-33g3, complete seq...    36   0.83   7
(M58295) P.yoelii circumsporozoite protein (CS) gene, comple...    44   0.84   3
(AL844506) Plasmodium falciparum chromosome  7.                    34   0.88   13
(CR847930) Zebrafish DNA sequence from clone DKEY-148H19 in ...    48   0.94   1
(BX537287) Zebrafish DNA sequence from clone CH211-206O8 in ...    48   0.94   1
(AY522493) Chiropterotriton sp. K clone E28.25 16S ribosomal...    48   0.94   1
(AY522492) Chiropterotriton sp. K clone E28.23 16S ribosomal...    48   0.94   1
(AY522491) Chiropterotriton sp. K clone E28.22 16S ribosomal...    48   0.94   1
(AB006745) Mus musculus gene for interleukin 15, exon 1 and ...    48   0.94   1
(AC087431) Homo sapiens chromosome 3 clone RP11-775C23 map 3...    48   0.94   1
(AC135822) Rattus norvegicus clone CH230-14E4, WORKING DRAFT...    48   0.94   1
(AC118397) Rattus norvegicus clone CH230-116A13, *** SEQUENC...    48   0.94   1
(AC106290) Rattus norvegicus clone CH230-70K10, WORKING DRAF...    48   0.94   1
(CT030007) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    48   0.94   1
(AC023353) Homo sapiens chromosome 20 clone RP11-775C23, WOR...    48   0.94   1
(DX561878) GH_MBb0070A06r GH_MBb Gossypium hirsutum genomic ...    48   0.94   1
(BZ175628) CH230-348M8.TJ CHORI-230 Segment 2 Rattus norvegi...    48   0.94   1
(AC115594) Dictyostelium discoideum chromosome 2 map 4071862...    40   0.94   8
(AC171130) Helobdella robusta clone CH306-1A12, complete seq...    38   1.0    6
(EX816322) CBNA4982.fwd CBNA Phycomyces blakesleeanus NRRL15...    40   1.0    2
(EX852098) CBNC9394.fwd CBNC Phycomyces blakesleeanus NRRL15...    40   1.0    2
(EX849716) CBNC8078.fwd CBNC Phycomyces blakesleeanus NRRL15...    40   1.1    2
(AE014848) Plasmodium falciparum 3D7 chromosome 12, section ...    30   1.2    15
(AC174344) Medicago truncatula clone mth2-167b8, complete se...    36   1.4    7
(AM462803) Vitis vinifera contig VV78X050124.4, whole genome...    42   1.6    2
(BG601807) EST500897 Plasmodium yoelii sporozoite cDNA Plasm...    44   2.1    2
(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...    34   2.2    11
(EJ272241) 1095353022197 Global-Ocean-Sampling_GS-27-01-01-1...    38   2.4    3
(BG603229) EST502311 Plasmodium yoelii sporozoite cDNA Plasm...    44   2.4    2
(CJ453170) Macaca fascicularis mRNA, clone: QflA-18857, 5' e...    34   2.5    2
(BG603835) EST502932 Plasmodium yoelii sporozoite cDNA Plasm...    44   2.7    2
(AF063866) Melanoplus sanguinipes entomopoxvirus, complete g...    36   3.1    13
(BG601087) EST500177 Plasmodium yoelii sporozoite cDNA Plasm...    44   3.1    2
(BG602513) EST501603 Plasmodium yoelii sporozoite cDNA Plasm...    44   3.1    2
(AE017308) Mycoplasma mobile 163K complete genome.                 32   3.3    18
(BG603505) EST502595 Plasmodium yoelii sporozoite cDNA Plasm...    44   3.5    2
(CF469234) P18C06 Plasmodium yoelli 17X axenic hepatic stage...    44   3.5    2
(CR936442) Zebrafish DNA sequence from clone DKEY-266F7 in l...    46   3.7    1
(CU207379) Mouse DNA sequence from clone CH29-505C15 on chro...    46   3.7    1
(BX855594) Mouse DNA sequence from clone RP24-322M2 on chrom...    46   3.7    1
(AC167021) Mus musculus BAC clone RP23-152D8 from chromosome...    46   3.7    1
(AC131738) Mus musculus BAC clone RP23-94E15 from 16, comple...    46   3.7    1
(AC115900) Mus musculus chromosome 6, clone RP24-464A7, comp...    46   3.7    1
(CU468239) S.lycopersicum DNA sequence from clone SL_MboI-12...    46   3.7    1
(AP009321) Solanum lycopersicum genomic DNA, chromosome 8, c...    46   3.7    1
(AM471981) Vitis vinifera, whole genome shotgun sequence, co...    46   3.7    1
(AC186753) Musa acuminata clone MA4_54B05, complete sequence.      46   3.7    1
(AC084297) Homo sapiens BAC clone RP11-44H9 from 2, complete...    46   3.7    1
(AC143315) Macaca mulatta clone CH250-270D18, *** SEQUENCING...    46   3.7    1
(AC128892) Rattus norvegicus clone CH230-54D1, *** SEQUENCIN...    46   3.7    1
(AC115648) Rattus norvegicus clone CH230-269G20, *** SEQUENC...    46   3.7    1
(AC115079) Mus musculus clone RP24-459D2, LOW-PASS SEQUENCE ...    46   3.7    1
(AC111464) Rattus norvegicus clone CH230-74E2, *** SEQUENCIN...    46   3.7    1
(AC107002) Rattus norvegicus clone CH230-110O3, *** SEQUENCI...    46   3.7    1
(CR385062) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    46   3.7    1
(AP003674) Mus musculus genomic DNA, chromosome 16q clone:RP...    46   3.7    1
(AP003663) Mus musculus genomic DNA, chromosome 16q clone:RP...    46   3.7    1
(AP003662) Mus musculus genomic DNA, chromosome 16q clone:RP...    46   3.7    1
(AC232506) Brassica rapa subsp. pekinensis clone KBrB059K16,...    46   3.7    1
(AC191917) Spermophilus tridecemlineatus clone VMRC20-379J4,...    46   3.7    1
(AC184621) Strongylocentrotus purpuratus clone R3-3074E13, W...    46   3.7    1
(AC181182) Strongylocentrotus purpuratus clone R3-14O14, WOR...    46   3.7    1
(AC175938) Strongylocentrotus purpuratus clone R3-17A3, WORK...    46   3.7    1
(AC166911) Glycine tomentella clone gtd1-36p21, WORKING DRAF...    46   3.7    1
(AC021009) Homo sapiens clone RP11-279H15, WORKING DRAFT SEQ...    46   3.7    1
(BH346715) CH230-54D1.TV CHORI-230 Segment 1 Rattus norvegic...    46   3.7    1
(ER545378) 1093016166513 Global-Ocean-Sampling_GS-35-01-01-1...    46   3.7    1
(EK233653) 1095460191018 Global-Ocean-Sampling_GS-31-01-01-1...    46   3.7    1
(DU025921) 6471 Tomato HindIII BAC Library Solanum lycopersi...    46   3.7    1
(DH075363) Oryzias latipes Fosmid clone:GOLWFno138_o19, forw...    46   3.7    1
(DE390636) Bombyx mori genomic DNA, BAC clone:12H8C.               46   3.7    1
(CZ961627) 308375 Tomato EcoRI BAC Library Solanum lycopersi...    46   3.7    1
(B20402) T22N9-T7 TAMU Arabidopsis thaliana genomic clone T2...    46   3.7    1
(AG318764) Mus musculus molossinus DNA, clone:MSMg01-104A20....    46   3.7    1
(AG280355) Mus musculus molossinus DNA, clone:MSMg01-051K14....    46   3.7    1
(EL912049) INIT2_49_D11.g1_A006 G5 trophont cDNA (INIT2) Ich...    46   3.7    1
(EL911967) INIT2_49_D11.b1_A006 G5 trophont cDNA (INIT2) Ich...    46   3.7    1
(BE850668) uw20h10.y1 Soares mouse 3NbMS Mus musculus cDNA c...    46   3.7    1
(EF189345) Plagiomnium arbuscula isolate CHI.1129 ribosomal ...    40   3.9    2
(EF189340) Plagiomnium arbuscula isolate CHI.1122 ribosomal ...    40   4.0    2
(DT233515) JGI_CAAT7665.rev CAAT Pimephales promelas brain 7...    44   4.2    2
(EB558334) AGENCOURT_51101791 D. virilis EST Drosophila viri...    42   4.2    2
(M22698) Plasmodium yoelii circumsporozoite protein gene (CS...    44   4.3    2
(CF469194) P17D1 Plasmodium yoelli 17X axenic hepatic stages...    44   4.3    2
(BJ334014) Dictyostelium discoideum cDNA clone:dda44g19, 5' ...    32   4.4    3
(BJ371575) Dictyostelium discoideum cDNA clone:ddc58i18, 5' ...    32   4.4    3
(CF469324) P2-H1 Plasmodium yoelli 17X axenic hepatic stages...    44   4.6    2
(CF468771) P10-B2 Plasmodium yoelli 17X axenic hepatic stage...    44   4.8    2
(ER446130) 1092963830021 Global-Ocean-Sampling_GS-35-01-01-1...    42   5.0    2
(CF469392) P20F12 Plasmodium yoelli 17X axenic hepatic stage...    44   5.3    2
(AE014842) Plasmodium falciparum 3D7 chromosome 11 section 7...    36   5.3    11
(CF469633) P24G04 Plasmodium yoelli 17X axenic hepatic stage...    44   5.4    2
(EU308518) Silene atocioides ribulose-1,5-bisphosphate carbo...    40   5.4    2
(FB571359) Sequence 226 from Patent EP1865317.                     32   5.8    9
(FB571303) Sequence 170 from Patent EP1865317.                     32   5.8    9
(CL081973) CH216-165E7_Sp5.1 CH216 Xenopus (Silurana) tropic...    36   5.9    2
(AC178731) Strongylocentrotus purpuratus clone R3-3002M6, WO...    36   6.1    6
(AC004157) Plasmodium falciparum chromosome 12 clone PFYAC29...    34   6.5    11
(AC004709) Plasmodium falciparum chromosome 12, *** SEQUENCI...    32   6.5    10
(AC150695) Bos taurus clone CH240-137A22, WORKING DRAFT SEQU...    32   6.7    2
(AJ277590) Dictyostelium discoideum iplA gene for inositol 1...    34   6.7    5
(AX344570) Sequence 21 from Patent WO0200932.                      34   6.9    11
(DQ158858) Bigelowiella natans nucleomorph chromosome 3, com...    38   7.0    7
(R68658) yi14c06.s1 Soares placenta Nb2HP Homo sapiens cDNA ...    40   7.3    2
(CR762469) Zebrafish DNA sequence from clone CH211-119N15 in...    32   7.3    8
(CT573399) Zebrafish DNA sequence from clone DKEYP-51B9 in l...    32   7.4    2
(AC168321) Strongylocentrotus purpuratus clone R3-64M23, WOR...    38   7.6    8
(AC117076) Dictyostelium discoideum chromosome 2 map 3323568...    32   7.7    10
(AC114263) Dictyostelium discoideum chromosome 2 map 215673-...    36   8.1    11
(AE014843) Plasmodium falciparum 3D7 chromosome 11 section 8...    38   8.7    11
(CR382400) Plasmodium falciparum chromosome 6, complete sequ...    34   8.7    12
(DX547430) GH_MBb0051G04r GH_MBb Gossypium hirsutum genomic ...    34   9.0    2
(EK265747) 1095462223992 Global-Ocean-Sampling_GS-31-01-01-1...    32   9.2    2
(CZ528347) SRAA-aac54h05.b1 Strongyloides ratti whole genome...    34   9.2    2
(EK294810) 1095462343690 Global-Ocean-Sampling_GS-31-01-01-1...    32   9.2    2
(AG390924) Mus musculus molossinus DNA, clone:MSMg01-207N09....    40   9.7    2
(ED374316) AUAC-aat62e12.b1 Ascaris suum whole genome shotgu...    32   9.9    3

>(C93972) Dictyostelium discoideum slug cDNA, clone SSC874.
          Length = 645

 Score = 1227 bits (619), Expect = 0.0
 Identities = 623/625 (99%)
 Strand = Plus / Plus


Query: 672  tttcaaataataacaatagtaaatcattgacagagtcggcaggtgcaattaatatcattg 731
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1    tttcaaataataacaatagtaaatcattgacagagtcggcaggtgcaattaatatcattg 60


Query: 732  attcttcaattataatagagtcttccacattcatcaataatagagcagccattggtggtg 791
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61   attcttcaattataatagagtcttccacattcatcaataatagagcagccattggtggtg 120


Query: 792  caattcaaatgaatggttcattacctgaacagtatgtaaaaatctataattcagtattta 851
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121  caattcaaatgaatggttcattacctgaacagtatgtaaaaatctataattcagtattta 180


Query: 852  atggtaataatgcaaccgatattggtggttctattttctctcaagagggtcaattatatc 911
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181  atggtaataatgcaaccgatattggtggttctattttctctcaagagggtcaattatatc 240


Query: 912  tctacagttgtcaattcttaaataatgaagcggttgctggttcatcagtttattgtttaa 971
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241  tctacagttgtcaattcttaaataatgaagcggttgctggttcatcagtttattgtttaa 300


Query: 972  attcaaatataaactttagtaatatgacatttaacaataataccgattcatcaataccaa 1031
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301  attcaaatataaactttagtaatatgacatttaacaataataccgattcatcaataccaa 360


Query: 1032 ctccaaatggtattggttgtggttctggtaaagtttgttccattcaaggtgatcaacaat 1091
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361  ctccaaatggtattggttgtggttctggtaaagtttgttccattcaaggtgatcaacaat 420


Query: 1092 ttcaaaatagttgttcctatgattatcaacctgataaaccatcattactatctcctggtg 1151
            ||||||||||||||||||||||||||||  ||||||||||||||||||||||||||||||
Sbjct: 421  ttcaaaatagttgttcctatgattatcannctgataaaccatcattactatctcctggtg 480


Query: 1152 aagtggctgcaatcgtaatttgtgttataattggtgctgctatcaatacaactgttttga 1211
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481  aagtggctgcaatcgtaatttgtgttataattggtgctgctatcaatacaactgttttga 540


Query: 1212 ttttaatcattcgtaaaattagtagaggaagaaatggttattccaaaattgttaatggtt 1271
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541  ttttaatcattcgtaaaattagtagaggaagaaatggttattccaaaattgttaatggtt 600


Query: 1272 aaacatttactactcgactttttat 1296
            |||||||||||||||||||||||||
Sbjct: 601  aaacatttactactcgactttttat 625

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 1,652,891,788
Number of extensions: 104309450
Number of successful extensions: 8684207
Number of sequences better than 10.0: 152
Length of query: 1416
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 1392
Effective length of database: 99,340,224,878
Effective search space: 138281593030176
Effective search space used: 138281593030176
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.18
Homology vs Protein 
Query= Contig-U01623-1 (Contig-U01623-1Q) /CSM_Contig/Contig-U01623-1Q.Seq.d
         (1416 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000678_461(CP000678|pid:none) Methanobrevibacter smithii ATCC ...    52   7e-05
CP000909_3832(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...    41   0.093
CP001337_33(CP001337|pid:none) Chloroflexus aggregans DSM 9485, ...    41   0.12
AE017226_1351(AE017226|pid:none) Treponema denticola ATCC 35405,...    39   0.46
CP000678_1188(CP000678|pid:none) Methanobrevibacter smithii ATCC...    39   0.60
CP000678_1585(CP000678|pid:none) Methanobrevibacter smithii ATCC...    37   1.8
CP000102_1066(CP000102|pid:none) Methanosphaera stadtmanae DSM 3...    37   1.8
AE017180_750(AE017180|pid:none) Geobacter sulfurreducens PCA, co...    37   2.3
CP000592_56(CP000592|pid:none) Ostreococcus lucimarinus CCE9901 ...    37   2.3
CP000678_411(CP000678|pid:none) Methanobrevibacter smithii ATCC ...    36   3.0
AC116979_55(AC116979|pid:none) Dictyostelium discoideum chromoso...    36   3.0
AP009552_100(AP009552|pid:none) Microcystis aeruginosa NIES-843 ...    36   3.0
AL646053_1537(AL646053|pid:none) Ralstonia solanacearum GMI1000 ...    36   3.9
AM889285_1982(AM889285|pid:none) Gluconacetobacter diazotrophicu...    35   6.7
CP000102_143(CP000102|pid:none) Methanosphaera stadtmanae DSM 30...    35   6.7
(Q9Z882) RecName: Full=Probable outer membrane protein pmp16; Al...    35   6.7
CP000850_4219(CP000850|pid:none) Salinispora arenicola CNS-205, ...    35   8.7
CP000806_2538(CP000806|pid:none) Cyanothece sp. ATCC 51142 circu...    35   8.7
CP000117_4696(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    35   8.7

>CP000678_461(CP000678|pid:none) Methanobrevibacter smithii ATCC
           35061, complete genome.
          Length = 1026

 Score = 51.6 bits (122), Expect = 7e-05
 Identities = 37/115 (32%), Positives = 51/115 (44%), Gaps = 4/115 (3%)
 Frame = +1

Query: 301 IKKELTTIGGIIFSNSTTLITINDCRFFNNSGNNGIGYIW---SGYLVMNNSLIEG-SNC 468
           I    T   G    NS + +T+N+C F NN+ NNG G I+   SG  V N S I   +N
Sbjct: 333 INNTATNDWGSAIYNSGSGLTVNNCSFINNTANNGAGAIYNTESGLTVSNCSFINNTANN 392

Query: 469 SPAFFFNSISGMGTGSPLFLISNTTFTNAIXXXXXXXXXSDSTTTFQQCTFSDNV 633
                +NS SG+        +SN +F N           S S +T   C F +N+
Sbjct: 393 GAGAIYNSGSGL-------TVSNCSFINNTANNGGAIHSSCSNSTIVNCNFINNI 440

 Score = 41.2 bits (95), Expect = 0.093
 Identities = 30/108 (27%), Positives = 46/108 (42%), Gaps = 6/108 (5%)
 Frame = +1

Query: 325 GGIIFSNSTTLITINDCRFFNNSGNNGIGYIWSGYLVMNNSLIEGSNCSPAFFFNSISGM 504
           GG    NS + +T+N+C F NN+  N     W   +  + S +  +NCS   F N+ +
Sbjct: 314 GGCAIYNSGSGLTVNNCSFINNTATND----WGSAIYNSGSGLTVNNCS---FINNTANN 366

Query: 505 GTGSPL-----FLISNTTF-TNAIXXXXXXXXXSDSTTTFQQCTFSDN 630
           G G+         +SN +F  N           S S  T   C+F +N
Sbjct: 367 GAGAIYNTESGLTVSNCSFINNTANNGAGAIYNSGSGLTVSNCSFINN 414

 Score = 38.5 bits (88), Expect = 0.60
 Identities = 33/104 (31%), Positives = 43/104 (41%), Gaps = 2/104 (1%)
 Frame = +1

Query: 325 GGIIFSNSTTLITINDCRFFNNSGNNGIGYIWSGYLVMNNSLIEGSNC--SPAFFFNSIS 498
           GG I S+ +    +N C F NN  NNG G I S     +NS I   N   + A    +I
Sbjct: 419 GGAIHSSCSNSTIVN-CNFINNIANNGAGAIHSS---CSNSTISSCNFINNTANHAGAIY 474

Query: 499 GMGTGSPLFLISNTTFTNAIXXXXXXXXXSDSTTTFQQCTFSDN 630
             G+ S +    N  F N I         S S +T   C F +N
Sbjct: 475 NAGSDSTMM---NCNFINNIANNGGAIHSSCSNSTIVNCNFINN 515

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,676,795,429
Number of extensions: 29073056
Number of successful extensions: 80838
Number of sequences better than 10.0: 19
Number of HSP's gapped: 80147
Number of HSP's successfully gapped: 21
Length of query: 472
Length of database: 1,051,180,864
Length adjustment: 132
Effective length of query: 340
Effective length of database: 623,955,076
Effective search space: 212144725840
Effective search space used: 212144725840
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.75  gvh: 0.41  alm: 0.20  top: 1.00  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.01  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for Contig-U01623-1 is cyt
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 2
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0