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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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Contig-U09621-1
Contig ID Contig-U09621-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U09621-1 (Contig-U09621-1Q) /CSM_Contig/Contig-U09621-1Q.Seq.d
AGACGTGACATCATAATTGATTTCGATAAATTTGTTTTAAATACATACAA
ATCTTTTATTTATTTTCTTAATTCAACACTGTAAATAACAAAAAAAAAAA
AAAANTAAAANTANTATTTGGGAANTAAAAAAAAA----------TATCT
TTTGGTGGTGTTAGTTTAGATCAAGCTTCACCAGTTGATTCAAATGTTTC
AATTAGATATGATGGTGTTGTATTATTTGTTTTCATTACATATTCAAATA
CATATACATATTCAACATCAGATTTTAAATATGTTTATTCAGTTCANCAA
ATTGCAAATACAATTTATNATGTACCAGAAACAATCATTTTAGAAAGTAT
TCANTCAAGATTACTTTATAAACGTCATGGTATTCGTGTTATTTTCATTC
AAACTGGNACAATTGGTTCTTTCCATTTCCAAACTTTATTATTAACTCTT
GTTTCAGGTCTTGGTTTATTAGCTGTAGCAACAACTGTTGTA
Gap gap included
Contig length 482
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 1145331
End point 1144848
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 2
Link to clone list U09621
List of clone(s) 

est1=VFG527F,1,136
est2=VFG527Z,137,484
Translated Amino Acid sequence 
dvts*lisinlf*ihtnllfifliqhck*QKKKKXKXXIWEXKK---

---SFGGVSLDQASPVDSNVSIRYDGVVLFVFITYSNTYTYSTSDFKYVYSVXQIANTIY
XVPETIILESIXSRLLYKRHGIRVIFIQTGTIGSFHFQTLLLTLVSGLGLLAVATTVV
Translated Amino Acid sequence (All Frames) 
Frame A:
rrdiiidfdkfvlntyksfiyflnstl*itkkkkx*xxylgxkkk---

---yllvvlv*iklhqliqmfqldmmvlyylfslhiqihihiqhqilnmfiqfxklqiqf
xmyqkqsf*kvfxqdyfinvmvfvlfsfklxqlvlsisklyy*llfqvlvy*l*qqll

Frame B:
dvts*lisinlf*ihtnllfifliqhck*QKKKKXKXXIWEXKK---

---ifwwc*frssfts*fkcfn*i*wcciicfhyifkyiyifnirf*iclfssxnckynl
xctrnnhfrkysxkitl*tswyscyfhsnwxnwffpfpnfiinscfrswfiscsnncc

Frame C:
t*hhn*fr*icfkyiqifylfs*fntvnnkkkkkxkxxfgx*kk---

---SFGGVSLDQASPVDSNVSIRYDGVVLFVFITYSNTYTYSTSDFKYVYSVXQIANTIY
XVPETIILESIXSRLLYKRHGIRVIFIQTGTIGSFHFQTLLLTLVSGLGLLAVATTVV
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U09621-1 (Contig-U09621-1Q)
/CSM_Contig/Contig-U09621-1Q.Seq.d
         (492 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U09621-1 (Contig-U09621-1Q) /CSM_Contig/Conti...   664  0.0
Contig-U12675-1 (Contig-U12675-1Q) /CSM_Contig/Conti...    36  0.034
Contig-U11414-1 (Contig-U11414-1Q) /CSM_Contig/Conti...    36  0.034
Contig-U14734-1 (Contig-U14734-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U11367-1 (Contig-U11367-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U11213-1 (Contig-U11213-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U11129-1 (Contig-U11129-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U08964-1 (Contig-U08964-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U05345-1 (Contig-U05345-1Q) /CSM_Contig/Conti...    34  0.13
Contig-U10467-1 (Contig-U10467-1Q) /CSM_Contig/Conti...    28  8.3

>Contig-U09621-1 (Contig-U09621-1Q) /CSM_Contig/Contig-U09621-1Q.Seq.d
          Length = 492

 Score =  664 bits (335), Expect = 0.0
 Identities = 347/347 (100%)
 Strand = Plus / Plus

                                                                       
Query: 146 tatcttttggtggtgttagtttagatcaagcttcaccagttgattcaaatgtttcaatta 205
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 146 tatcttttggtggtgttagtttagatcaagcttcaccagttgattcaaatgtttcaatta 205

                                                                       
Query: 206 gatatgatggtgttgtattatttgttttcattacatattcaaatacatatacatattcaa 265
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 206 gatatgatggtgttgtattatttgttttcattacatattcaaatacatatacatattcaa 265

                                                                       
Query: 266 catcagattttaaatatgtttattcagttcancaaattgcaaatacaatttatnatgtac 325
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 266 catcagattttaaatatgtttattcagttcancaaattgcaaatacaatttatnatgtac 325

                                                                       
Query: 326 cagaaacaatcattttagaaagtattcantcaagattactttataaacgtcatggtattc 385
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 326 cagaaacaatcattttagaaagtattcantcaagattactttataaacgtcatggtattc 385

                                                                       
Query: 386 gtgttattttcattcaaactggnacaattggttctttccatttccaaactttattattaa 445
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 386 gtgttattttcattcaaactggnacaattggttctttccatttccaaactttattattaa 445

                                                          
Query: 446 ctcttgtttcaggtcttggtttattagctgtagcaacaactgttgta 492
           |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 446 ctcttgtttcaggtcttggtttattagctgtagcaacaactgttgta 492


 Score =  176 bits (89), Expect = 1e-44
 Identities = 89/89 (100%)
 Strand = Plus / Plus

                                                                      
Query: 1  agacgtgacatcataattgatttcgataaatttgttttaaatacatacaaatcttttatt 60
          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  agacgtgacatcataattgatttcgataaatttgttttaaatacatacaaatcttttatt 60

                                       
Query: 61 tattttcttaattcaacactgtaaataac 89
          |||||||||||||||||||||||||||||
Sbjct: 61 tattttcttaattcaacactgtaaataac 89


>Contig-U12675-1 (Contig-U12675-1Q) /CSM_Contig/Contig-U12675-1Q.Seq.d
          Length = 830

 Score = 36.2 bits (18), Expect = 0.034
 Identities = 18/18 (100%)
 Strand = Plus / Minus

                             
Query: 54  ttttatttattttcttaa 71
           ||||||||||||||||||
Sbjct: 732 ttttatttattttcttaa 715


>Contig-U11414-1 (Contig-U11414-1Q) /CSM_Contig/Contig-U11414-1Q.Seq.d
          Length = 1931

 Score = 36.2 bits (18), Expect = 0.034
 Identities = 18/18 (100%)
 Strand = Plus / Minus

                              
Query: 210  tgatggtgttgtattatt 227
            ||||||||||||||||||
Sbjct: 1406 tgatggtgttgtattatt 1389


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 11,182
Number of Sequences: 6905
Number of extensions: 11182
Number of successful extensions: 1265
Number of sequences better than 10.0: 300
length of query: 492
length of database: 5,674,871
effective HSP length: 16
effective length of query: 476
effective length of database: 5,564,391
effective search space: 2648650116
effective search space used: 2648650116
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.11.16
Homology vs DNA 
Query= Contig-U09621-1 (Contig-U09621-1Q) /CSM_Contig/Contig-U09621-1Q.Seq.d
         (492 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...   599   0.0    3
(EU047552) Dictyostelium discoideum P2X receptor ATP-gated i...   664   0.0    1
(BJ431472) Dictyostelium discoideum cDNA clone:ddv14e07, 3' ...   664   0.0    1
(BJ413130) Dictyostelium discoideum cDNA clone:ddv14e07, 5' ...   176   5e-40  1
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    48   2e-26  6
(AC126783) Medicago truncatula clone mth2-15k17, complete se...    50   0.075  1
(AC111852) Rattus norvegicus clone CH230-12H7, *** SEQUENCIN...    50   0.075  1
(CW858582) shei4-61.g_055.ab1 Whole-genome shotgun library o...    50   0.075  1
(CU468572) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   0.14   4
(CU928530) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    40   0.22   3
(AG035415) Pan troglodytes DNA, clone: PTB-011A22.F.               36   0.22   2
(CU210932) Mouse DNA sequence from clone DN-106G2 on chromos...    48   0.30   1
(AL928632) Mouse DNA sequence from clone RP23-303M15 on chro...    48   0.30   1
(AC167164) Mus musculus chromosome 7, clone RP24-276M8, comp...    48   0.30   1
(AC110911) Mus musculus chromosome 7, clone RP24-357A15, com...    48   0.30   1
(CU655913) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.30   1
(CU606941) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.30   1
(AL772189) Zebrafish DNA sequence from clone CH211-135B6 in ...    38   0.61   5
(AC012061) Homo sapiens BAC clone CTD-2052D1 from 7, complet...    40   0.79   3
(AC189489) Brassica rapa subsp. pekinensis clone KBrB084K02,...    40   0.79   5
(AC130449) Homo sapiens chromosome 16 clone CTA-233A7, compl...    38   0.84   3
(AC002399) Homo sapiens clone A-481B3, *** SEQUENCING IN PRO...    38   0.94   4
(AC009030) Homo sapiens chromosome 16 clone RP11-13G6, compl...    38   0.96   3
(AL928901) Zebrafish DNA sequence from clone CH211-155M12 in...    36   1.0    6
(AC138611) Mus musculus BAC clone RP23-41P19 from chromosome...    46   1.2    1
(AC122118) Mus musculus chromosome 18, clone RP24-225P17, co...    46   1.2    1
(AC189734) Trichinella spiralis BAC clone TS195-20H12 from c...    46   1.2    1
(AL391383) Human DNA sequence from clone RP11-532O21 on chro...    46   1.2    1
(AC068112) Homo sapiens chromosome 18, clone RP11-526H11, co...    46   1.2    1
(AC162480) Bos taurus clone CH240-119E24, WORKING DRAFT SEQU...    46   1.2    1
(AC157172) Bos taurus clone CH240-67L12, WORKING DRAFT SEQUE...    46   1.2    1
(AC145017) Felis catus clone RP86-269K3, WORKING DRAFT SEQUE...    46   1.2    1
(AC136938) Homo sapiens chromosome 16 clone RP11-1152A8, WOR...    46   1.2    1
(CU914339) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.2    1
(CU896597) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.2    1
(AC090212) Homo sapiens chromosome 18 clone RP11-760C16 map ...    46   1.2    1
(CU074395) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.2    1
(AC230302) Bos taurus clone CH240-513G12, WORKING DRAFT SEQU...    46   1.2    1
(AC222893) Bos taurus clone CH240-436A11, WORKING DRAFT SEQU...    46   1.2    1
(AC170956) Bos taurus clone CH240-262P21, WORKING DRAFT SEQU...    46   1.2    1
(AC165740) Bos taurus clone CH240-161B17, WORKING DRAFT SEQU...    46   1.2    1
(AC021509) Homo sapiens chromosome 18 clone RP11-255L11 map ...    46   1.2    1
(ET692328) CHO_OF332xm22r1.ab1 CHO_OF Nicotiana tabacum geno...    46   1.2    1
(DE399907) Bombyx mori genomic DNA, BAC clone:1B2D.                46   1.2    1
(CC480433) CH240_307F12.T7 CHORI-240 Bos taurus genomic clon...    46   1.2    1
(CO638301) USDA-FP_107038 Adult Glassy-winged Sharpshooter H...    46   1.2    1
(CO130657) GR__Eb33I08.r GR__Eb Gossypium raimondii cDNA clo...    46   1.2    1
(CO130656) GR__Eb33I08.f GR__Eb Gossypium raimondii cDNA clo...    46   1.2    1
(CO104240) GR__Eb0033I08.f GR__Eb Gossypium raimondii cDNA c...    46   1.2    1
(BW642990) Dugesia ryukyuensis mRNA, clone: Dr_sW_026_A21, 5...    46   1.2    1
(BW641387) Dugesia ryukyuensis mRNA, clone: Dr_sW_021_C07, 5...    46   1.2    1
(BX950199) Zebrafish DNA sequence from clone CH211-215A11 in...    40   1.2    3
(AC106815) Homo sapiens chromosome 16 clone RP11-626G21, WOR...    40   1.3    2
(AC168454) Strongylocentrotus purpuratus clone R3-35M20, WOR...    32   1.3    7
(AC013718) Homo sapiens BAC clone RP11-116I17 from 2, comple...    40   1.3    2
(AC098527) Rattus norvegicus clone CH230-82N17, WORKING DRAF...    40   1.3    5
(AZ887478) RPCI-24-167O24.TV RPCI-24 Mus musculus genomic cl...    40   1.4    2
(CR384075) Zebrafish DNA sequence from clone DKEY-231E3 in l...    36   1.5    5
(AC133062) Rattus norvegicus clone CH230-4H17, WORKING DRAFT...    40   1.7    2
(ED880734) OA_CBa0034O09.f OA_CBa Oryza australiensis genomi...    40   1.8    2
(AP003403) Homo sapiens genomic DNA, chromosome 11 clone:RP1...    34   1.9    5
(AC109197) Mus musculus chromosome 1, clone RP23-309A8, comp...    32   2.3    2
(AC150616) Callithrix jacchus clone CH259-471J6, WORKING DRA...    34   2.7    4
(AC179014) Strongylocentrotus purpuratus clone R3-32I23, WOR...    34   3.1    6
(CP000312) Clostridium perfringens SM101, complete genome.         40   3.6    15
(AC230428) Bos taurus clone CH240-509B20, WORKING DRAFT SEQU...    38   4.6    5
(AC161572) Mus musculus chromosome 15, clone RP23-358J10, co...    44   4.6    1
(AC159631) Mus musculus BAC clone RP24-177F21 from chromosom...    44   4.6    1
(AC158382) Mus musculus BAC clone RP24-105N17 from chromosom...    44   4.6    1
(AC158365) Mus musculus BAC clone RP23-182N12 from chromosom...    44   4.6    1
(AC134468) Mus musculus BAC clone RP23-97J21 from chromosome...    44   4.6    1
(AC122293) Mus musculus BAC clone RP23-257O9 from 10, comple...    44   4.6    1
(AC102647) Mus musculus chromosome 10, clone RP23-269A24, co...    44   4.6    1
(AC100738) Mus musculus chromosome 15, clone RP24-362K21, co...    44   4.6    1
(EF461010) Helianthus annuus clone ZVG17_ZENA17-F ZVG17 locu...    44   4.6    1
(DQ336955) Glycine max cultivar Williams 82 clone BAC gmw1-5...    44   4.6    1
(AM437607) Vitis vinifera contig VV78X235706.4, whole genome...    44   4.6    1
(AM430366) Vitis vinifera contig VV78X201612.3, whole genome...    44   4.6    1
(AM423271) Vitis vinifera contig VV78X118542.7, whole genome...    44   4.6    1
(CT737383) Pig DNA sequence from clone CH242-215D15 on chrom...    44   4.6    1
(AE013599) Drosophila melanogaster chromosome 2R, complete s...    44   4.6    1
(AC005713) Drosophila melanogaster, chromosome 2R, region 54...    44   4.6    1
(AC145047) Sus scrofa clone RP44-44B5, WORKING DRAFT SEQUENC...    44   4.6    1
(AC133878) Mus musculus chromosome 10 clone RP24-219D21 map ...    44   4.6    1
(AC107465) Rattus norvegicus clone CH230-86H10, *** SEQUENCI...    44   4.6    1
(AC183079) Bos taurus clone CH240-75A22, WORKING DRAFT SEQUE...    44   4.6    1
(AC183076) Bos taurus clone CH240-77J8, WORKING DRAFT SEQUEN...    44   4.6    1
(AC169446) Bos taurus clone CH240-196H18, WORKING DRAFT SEQU...    44   4.6    1
(AC165568) Bos taurus clone CH240-161H6, WORKING DRAFT SEQUE...    44   4.6    1
(AC014268) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    44   4.6    1
(AC007835) Drosophila melanogaster strain y chromosome 2 clo...    44   4.6    1
(EI080053) hgy0002ad11.b1 hgy Oryza officinalis genomic clon...    44   4.6    1
(ED801426) MG__Ba0018E16f MG__Ba Mimulus guttatus genomic, g...    44   4.6    1
(DU521707) 1098609853651 CHORI-243 Ovis aries genomic clone ...    44   4.6    1
(DE417863) Bombyx mori genomic DNA, BAC clone:18C1B.               44   4.6    1
(DE394301) Bombyx mori genomic DNA, BAC clone:15J10F.              44   4.6    1
(DE113304) Oryzias latipes DNA, forward end of BAC clone: Md...    44   4.6    1
(CU764119) A BAC library has been constructed from PN40024 g...    44   4.6    1
(AG280087) Mus musculus molossinus DNA, clone:MSMg01-051E06....    44   4.6    1
(EG668883) RCRC955TP Castor bean cDNA library from roots, 1....    44   4.6    1

>(AC115599) Dictyostelium discoideum chromosome 2 map 4229098-4354721
             strain AX4, complete sequence.
          Length = 125623

 Score =  599 bits (302), Expect(3) = 0.0
 Identities = 310/314 (98%)
 Strand = Plus / Minus


Query: 179   caccagttgattcaaatgtttcaattagatatgatggtgttgtattatttgttttcatta 238
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 95671 caccagttgattcaaatgtttcaattagatatgatggtgttgtattatttgttttcatta 95612


Query: 239   catattcaaatacatatacatattcaacatcagattttaaatatgtttattcagttcanc 298
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct: 95611 catattcaaatacatatacatattcaacatcagattttaaatatgtttattcagttcaac 95552


Query: 299   aaattgcaaatacaatttatnatgtaccagaaacaatcattttagaaagtattcantcaa 358
             |||||||||||||||||||| |||||||||||||||||||||||||||||||||| ||||
Sbjct: 95551 aaattgcaaatacaatttatgatgtaccagaaacaatcattttagaaagtattcattcaa 95492


Query: 359   gattactttataaacgtcatggtattcgtgttattttcattcaaactggnacaattggtt 418
             ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 95491 gattactttataaacgtcatggtattcgtgttattttcattcaaactggtacaattggtt 95432


Query: 419   ctttccatttccaaactttattattaactcttgtttcaggtcttggtttattagctgtag 478
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 95431 ctttccatttccaaactttattattaactcttgtttcaggtcttggtttattagctgtag 95372


Query: 479   caacaactgttgta 492
             ||||||||||||||
Sbjct: 95371 caacaactgttgta 95358

 Score =  176 bits (89), Expect(3) = 0.0
 Identities = 89/89 (100%)
 Strand = Plus / Minus


Query: 1     agacgtgacatcataattgatttcgataaatttgttttaaatacatacaaatcttttatt 60
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96809 agacgtgacatcataattgatttcgataaatttgttttaaatacatacaaatcttttatt 96750


Query: 61    tattttcttaattcaacactgtaaataac 89
             |||||||||||||||||||||||||||||
Sbjct: 96749 tattttcttaattcaacactgtaaataac 96721

 Score = 65.9 bits (33), Expect(3) = 0.0
 Identities = 33/33 (100%)
 Strand = Plus / Minus


Query: 146   tatcttttggtggtgttagtttagatcaagctt 178
             |||||||||||||||||||||||||||||||||
Sbjct: 95827 tatcttttggtggtgttagtttagatcaagctt 95795

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 603,261,875
Number of extensions: 45446294
Number of successful extensions: 4265179
Number of sequences better than 10.0: 164
Length of query: 492
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 469
Effective length of database: 93,106,754,628
Effective search space: 43667067920532
Effective search space used: 43667067920532
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 6.26
Homology vs Protein 
Query= Contig-U09621-1 (Contig-U09621-1Q) /CSM_Contig/Contig-U09621-1Q.Seq.d
         (492 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115599_40(AC115599|pid:none) Dictyostelium discoideum chromoso...   156   2e-37
AC116305_39(AC116305|pid:none) Dictyostelium discoideum chromoso...   102   5e-21

>AC115599_40(AC115599|pid:none) Dictyostelium discoideum chromosome
           2 map 4229098-4354721 strain AX4, complete sequence.
           &EU047552_1(EU047552|pid:none)
          Length = 378

 Score =  156 bits (395), Expect = 2e-37
 Identities = 85/115 (73%), Positives = 85/115 (73%)
 Frame = +1

Query: 148 SFGGVSLDQASPVDSNVSIRYDGVVLFVFIXXXXXXXXXXXDFKYVYSVXQIANTIYXVP 327
           SFGGVSLDQASPVDSNVSIRYDGVVLFVFI           DFKYVYSV QIANTIY VP
Sbjct: 215 SFGGVSLDQASPVDSNVSIRYDGVVLFVFITYSNTYTYSTSDFKYVYSVQQIANTIYDVP 274

Query: 328 ETIILESIXSRLLYKRHGIRVIFIQTGTIGSFHFQXXXXXXXXXXXXXXXXTTVV 492
           ETIILESI SRLLYKRHGIRVIFIQTGTIGSFHFQ                TTVV
Sbjct: 275 ETIILESIHSRLLYKRHGIRVIFIQTGTIGSFHFQTLLLTLVSGLGLLAVATTVV 329

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 506,128,651
Number of extensions: 6833745
Number of successful extensions: 9780
Number of sequences better than 10.0: 2
Number of HSP's gapped: 9779
Number of HSP's successfully gapped: 2
Length of query: 164
Length of database: 1,040,966,779
Length adjustment: 118
Effective length of query: 46
Effective length of database: 662,861,149
Effective search space: 30491612854
Effective search space used: 30491612854
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.54  gvh: 0.50  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.15  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.09  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 16.0 %: vesicles of secretory system
	 16.0 %: nuclear
	 12.0 %: plasma membrane
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for Contig-U09621-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 1
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0