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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U01586-1
Contig ID Contig-U01586-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U01586-1 (Contig-U01586-1Q) /CSM_Contig/Contig-U01586-1Q.Seq.d
ATTCAATAACCATTATTATTATTTTTATTATAATTATTTTAAAATTTTTA
TTTTATTAATTTATTTATTTATTCAATTATTAATTTTTTGGTTGCCACTG
GTTGCCGACTAAAACCAACTAAAAAAAAAAAAAAAAAAAACCAAAATAAT
TTATCCCCATTAAATAATTATAGAGATATTGGAAAAAAAATGAAAACTAT
ATTATCATTATTATTTATTTTAAGTATTTTTTCAAATTTATATTTTGTTA
AATCGCAACAATCGGTTACAGTACTTTCAGATTCAATTGAATCATTTGGA
ACAATTGAAACTGTTAAATCAGGTGCTTCAATTTACTCTTTCACATTGAC
CTCCAAATTACCAACCAACATTACTGTAACACCATGTTTTGGAACTTTCG
ATATTTATGTTAGTCTAAACAATATTATAACAAATAAGAGTGCAACCTAT
TATGGTGAATGGGATCCATCTGTAAAAGCACTTGGAACAAATTTAAAAGC
ATTACCTGAAAATACTATTATCTATATTGCTGTTATGCCATTAGAAGAAT
ATGATTTCAAAAACAAAACATATGCCGCATCCTTCTCTGTTTTAATCACT
ACAAATGAACAAGATCTTGTTGTTCCATATTTACCAATACCAGCAAGA--
--------GTTCCATATTTACCAATACCAGCAAGAGATGGTGGTGTTGAT
ATTAAATTAACAAATGGTGGTAAATCAGGTACAATTAGTTTTTATAAAAC
TGGAAATTCTACTGATAATTATACAATTTACACTTTTTATGGTACTAGAC
CAAAAGGAAGTTTCCCATTCTCTGCATGTGGTGTTAAATTTTATGCAACT
GAAAACAATCAAAAGATTGAAGATAATGGTGACAATGAAGCATCTGCTAC
TTTCACTGGTTTAAATACTAAAGAACCAACTGGTTTAACTGTTGTTGTTA
CTAGAAAGACAAAGAATTTTTCAAGTGCCTATGATTATGCTGTTTTTAAT
GCTCCAAATTCTTCATCTTCTTTAAAATCAACTTTCTTAAATTTAATTCC
AGCTTTCTTTATTTGTATTTTTATTTCATTTTTATTATTGTAAAACCACA
ATAATCTTCACATATTTCATTTGTTTTTTTTTTTTCTGGAAAAAAAAAAA
AAAAGCTTAACAAAATAAAATAAATATTTAATAACAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Gap gap included
Contig length 1227
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 2361589
End point 2362818
Strand (PLUS/MINUS) PLUS
Number of clones 4
Number of EST 5
Link to clone list U01586
List of clone(s) 

est1=SSC180F,1,607
est2=VSG740F,181,649
est3=SSC180Z,650,1178
est4=SSJ552F,725,1229
est5=SSK272Z,731,1204
Translated Amino Acid sequence 
iq*plllfll*lf*NFYFINLFIYSIINFLVATGCRLKPTKKKKKKNQNNLSPLNNYRDI
GKKMKTILSLLFILSIFSNLYFVKSQQSVTVLSDSIESFGTIETVKSGASIYSFTLTSKL
PTNITVTPCFGTFDIYVSLNNIITNKSATYYGEWDPSVKALGTNLKALPENTIIYIAVMP
LEEYDFKNKTYAASFSVLITTNEQDLVVPYLPIPAR---

---VPYLPIPARDGGVDIKLTNGGKSGTISFYKTGNSTDNYTIYTFYGTRPKGSFPFSAC
GVKFYATENNQKIEDNGDNEASATFTGLNTKEPTGLTVVVTRKTKNFSSAYDYAVFNAPN
SSSSLKSTFLNLIPAFFICIFISFLLL*nhnnlhifhlfffflekkkksltk*nkylitk
kkkkkkkkkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
iq*plllfll*lf*NFYFINLFIYSIINFLVATGCRLKPTKKKKKKNQNNLSPLNNYRDI
GKKMKTILSLLFILSIFSNLYFVKSQQSVTVLSDSIESFGTIETVKSGASIYSFTLTSKL
PTNITVTPCFGTFDIYVSLNNIITNKSATYYGEWDPSVKALGTNLKALPENTIIYIAVMP
LEEYDFKNKTYAASFSVLITTNEQDLVVPYLPIPAR---

---VPYLPIPARDGGVDIKLTNGGKSGTISFYKTGNSTDNYTIYTFYGTRPKGSFPFSAC
GVKFYATENNQKIEDNGDNEASATFTGLNTKEPTGLTVVVTRKTKNFSSAYDYAVFNAPN
SSSSLKSTFLNLIPAFFICIFISFLLL*nhnnlhifhlfffflekkkksltk*nkylitk
kkkkkkkkkkkkkkkk

Frame B:
fnnhyyyfyynyfkifilliylfiqllifwlplvad*nqlkkkkkktkiiyph*iiieil
ekk*klyyhyylf*vffqiyillnrnnrlqyfqiqlnhleqlkllnqvlqftlsh*ppny
qptll*hhvlelsifmlv*til*qirvqpimvngihl*khleqi*khylkillsilllch
*knmisktkhmphpslf*slqmnkillfhiyqyqq---

---fhiyqyqqemvvliln*qmvvnqvqlvfikleilliiiqftlfmvldqkevshslhv
vlnfmqlktikrlkimvtmkhlllslv*ilknqlv*lllllerqrifqvpmimlflmlqi
lhll*nqls*i*fqlslfvflfhfyyckttiiftyficffffwkkkkka*qnkini**qk
kkkkkkkkkkkkkkk

Frame C:
sitiiiifiiiilkflfy*fiylfny*ffgchwlptktn*kkkkkkpk*fipik*l*ryw
kknenyiiiiiyfkyffkfifc*iatigystfrfn*iiwnn*nc*ircfnllfhidlqit
nqhycntmfwnfrylc*skqyynk*ecnllw*mgsickstwnkfksit*kyyylyccyai
rri*fqkqnicrillcfnhyk*trsccsiftntsk---

---siftntskrwwc*y*inkww*iryn*fl*nwkfy**lynlhflwy*tkrkfpilcmw
c*ilcn*kqskd*r*w*q*sicyfhwfky*rtnwfncccy*kdkeffkcl*lccf*cskf
fiffkinflkfnssflylyfyfifiivkpq*sshisfvfffsgkkkkklnkik*ifnnkk
kkkkkkkkkkkkkkk
own update 2004. 6. 7
Homology vs CSM-cDNA 
Query= Contig-U01586-1 (Contig-U01586-1Q)
/CSM_Contig/Contig-U01586-1Q.Seq.d
         (1237 letters)

Database: CSM
           8361 sequences; 7,895,291 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U01586-1 (Contig-U01586-1Q) /CSM_Contig/Conti...  1493  0.0
Contig-U14497-1 (Contig-U14497-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U10755-1 (Contig-U10755-1Q) /CSM_Contig/Conti...    42  0.002
Contig-U12913-1 (Contig-U12913-1Q) /CSM_Contig/Conti...    40  0.008
Contig-U11600-1 (Contig-U11600-1Q) /CSM_Contig/Conti...    38  0.031
Contig-U09080-1 (Contig-U09080-1Q) /CSM_Contig/Conti...    38  0.031
Contig-U05167-1 (Contig-U05167-1Q) /CSM_Contig/Conti...    38  0.031
Contig-U02677-1 (Contig-U02677-1Q) /CSM_Contig/Conti...    38  0.031
Contig-U14745-1 (Contig-U14745-1Q) /CSM_Contig/Conti...    36  0.12
Contig-U14734-1 (Contig-U14734-1Q) /CSM_Contig/Conti...    36  0.12

>Contig-U01586-1 (Contig-U01586-1Q) /CSM_Contig/Contig-U01586-1Q.Seq.d
          Length = 1237

 Score = 1493 bits (753), Expect = 0.0
 Identities = 783/783 (100%)
 Strand = Plus / Plus

                                                                        
Query: 247  gttaaatcgcaacaatcggttacagtactttcagattcaattgaatcatttggaacaatt 306
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 247  gttaaatcgcaacaatcggttacagtactttcagattcaattgaatcatttggaacaatt 306

                                                                        
Query: 307  gaaactgttaaatcaggtgcttcaatttactctttcacattgacctccaaattaccaacc 366
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 307  gaaactgttaaatcaggtgcttcaatttactctttcacattgacctccaaattaccaacc 366

                                                                        
Query: 367  aacattactgtaacaccatgttttggaactttcgatatttatgttagtctaaacaatatt 426
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 367  aacattactgtaacaccatgttttggaactttcgatatttatgttagtctaaacaatatt 426

                                                                        
Query: 427  ataacaaataagagtgcaacctattatggtgaatgggatccatctgtaaaagcacttgga 486
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 427  ataacaaataagagtgcaacctattatggtgaatgggatccatctgtaaaagcacttgga 486

                                                                        
Query: 487  acaaatttaaaagcattacctgaaaatactattatctatattgctgttatgccattagaa 546
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 487  acaaatttaaaagcattacctgaaaatactattatctatattgctgttatgccattagaa 546

                                                                        
Query: 547  gaatatgatttcaaaaacaaaacatatgccgcatccttctctgttttaatcactacaaat 606
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 547  gaatatgatttcaaaaacaaaacatatgccgcatccttctctgttttaatcactacaaat 606

                                                                        
Query: 607  gaacaagatcttgttgttccatatttaccaataccagcaagannnnnnnnnngttccata 666
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 607  gaacaagatcttgttgttccatatttaccaataccagcaagannnnnnnnnngttccata 666

                                                                        
Query: 667  tttaccaataccagcaagagatggtggtgttgatattaaattaacaaatggtggtaaatc 726
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 667  tttaccaataccagcaagagatggtggtgttgatattaaattaacaaatggtggtaaatc 726

                                                                        
Query: 727  aggtacaattagtttttataaaactggaaattctactgataattatacaatttacacttt 786
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 727  aggtacaattagtttttataaaactggaaattctactgataattatacaatttacacttt 786

                                                                        
Query: 787  ttatggtactagaccaaaaggaagtttcccattctctgcatgtggtgttaaattttatgc 846
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 787  ttatggtactagaccaaaaggaagtttcccattctctgcatgtggtgttaaattttatgc 846

                                                                        
Query: 847  aactgaaaacaatcaaaagattgaagataatggtgacaatgaagcatctgctactttcac 906
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 847  aactgaaaacaatcaaaagattgaagataatggtgacaatgaagcatctgctactttcac 906

                                                                        
Query: 907  tggtttaaatactaaagaaccaactggtttaactgttgttgttactagaaagacaaagaa 966
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 907  tggtttaaatactaaagaaccaactggtttaactgttgttgttactagaaagacaaagaa 966

                                                                        
Query: 967  tttttcaagtgcctatgattatgctgtttttaatgctccaaattcttcatcttctttaaa 1026
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 967  tttttcaagtgcctatgattatgctgtttttaatgctccaaattcttcatcttctttaaa 1026

               
Query: 1027 atc 1029
            |||
Sbjct: 1027 atc 1029


 Score = 83.8 bits (42), Expect = 6e-16
 Identities = 42/42 (100%)
 Strand = Plus / Plus

                                                     
Query: 141 ccaaaataatttatccccattaaataattatagagatattgg 182
           ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 141 ccaaaataatttatccccattaaataattatagagatattgg 182


 Score = 67.9 bits (34), Expect = 3e-11
 Identities = 34/34 (100%)
 Strand = Plus / Plus

                                              
Query: 1090 gtaaaaccacaataatcttcacatatttcatttg 1123
            ||||||||||||||||||||||||||||||||||
Sbjct: 1090 gtaaaaccacaataatcttcacatatttcatttg 1123


 Score = 61.9 bits (31), Expect = 2e-09
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 90  ggttgccactggttgccgactaaaaccaact 120
           |||||||||||||||||||||||||||||||
Sbjct: 90  ggttgccactggttgccgactaaaaccaact 120


 Score = 61.9 bits (31), Expect = 2e-09
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                           
Query: 1155 gcttaacaaaataaaataaatatttaataac 1185
            |||||||||||||||||||||||||||||||
Sbjct: 1155 gcttaacaaaataaaataaatatttaataac 1185


>Contig-U14497-1 (Contig-U14497-1Q) /CSM_Contig/Contig-U14497-1Q.Seq.d
          Length = 1748

 Score = 44.1 bits (22), Expect = 5e-04
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                  
Query: 1163 aaataaaataaatatttaataa 1184
            ||||||||||||||||||||||
Sbjct: 25   aaataaaataaatatttaataa 4


>Contig-U10755-1 (Contig-U10755-1Q) /CSM_Contig/Contig-U10755-1Q.Seq.d
          Length = 1532

 Score = 42.1 bits (21), Expect = 0.002
 Identities = 33/37 (89%)
 Strand = Plus / Plus

                                                
Query: 688 tggtggtgttgatattaaattaacaaatggtggtaaa 724
           |||||||| ||||||||||||||| || | |||||||
Sbjct: 247 tggtggtggtgatattaaattaactaaagatggtaaa 283


  Database: CSM
    Posted date:  Jun 7, 2004  8:33 AM
  Number of letters in database: 7,895,291
  Number of sequences in database:  8361
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 23,215
Number of Sequences: 8361
Number of extensions: 23215
Number of successful extensions: 4102
Number of sequences better than 10.0: 415
length of query: 1237
length of database: 7,895,291
effective HSP length: 16
effective length of query: 1221
effective length of database: 7,761,515
effective search space: 9476809815
effective search space used: 9476809815
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 5. 2
Homology vs DNA 
Query= Contig-U01586-1 (Contig-U01586-1Q) /CSM_Contig/Contig-U01586-1Q.Seq.d
         (1237 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU266771) Dictyostelium discoideum vegetative cDNA clone:VS...   797   0.0    1
(C92028) Dictyostelium discoideum slug cDNA, clone SSC180.        708   0.0    1
(C91453) Dictyostelium discoideum slug cDNA, clone SSK272.        541   e-149  1
(AU074117) Dictyostelium discoideum slug cDNA, clone SSJ552.      476   e-129  1
(AU071645) Dictyostelium discoideum slug cDNA, clone SSC180.       84   3e-24  2
(X80245) Locusta migratoria mitochondrion complete genome.         46   0.26   2
(AC169562) Bos taurus clone CH240-221A19, WORKING DRAFT SEQU...    48   0.82   1
(AE017332) Mycoplasma hyopneumoniae 232, complete genome.          48   0.82   1
(AE017244) Mycoplasma hyopneumoniae 7448, complete genome.         48   0.82   1
(AE017243) Mycoplasma hyopneumoniae J, complete genome.            48   0.82   1
(AC220477) Bos taurus clone CH240-357E22, WORKING DRAFT SEQU...    46   1.2    5
(CR788251) Zebrafish DNA sequence from clone DKEY-231F24 in ...    34   1.7    2
(CU640419) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    40   2.6    5
(CT025547) Mouse DNA sequence from clone RP24-111B7 on chrom...    46   3.2    1
(EU513374) Oedaleus decorus asiaticus mitochondrion, complet...    46   3.2    1
(EU366091) Locusta migratoria manilensis voucher H5042 cytoc...    46   3.2    1
(EU287446) Locusta migratoria migratoria mitochondrion, comp...    46   3.2    1
(EF151865) Oedaleus decorus asiaticus cytochrome oxidase sub...    46   3.2    1
(EF151834) Oedaleus decorus cytochrome oxidase subunit I (CO...    46   3.2    1
(AY318912) Locusta sp. JD-2003 cytochrome oxidase subunit I ...    46   3.2    1
(AC147958) Mus musculus chromosome 7 clone RP23-205L17 map 7...    46   3.2    1
(CU207363) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    46   3.2    1
(EJ456189) 1093017482155 Global-Ocean-Sampling_GS-28-01-01-1...    46   3.2    1
(AG039436) Pan troglodytes DNA, clone: PTB-016I16.R.               46   3.2    1
(EE233250) LB02117.CR_E18 GC_BGC-21 Bos taurus cDNA clone IM...    46   3.2    1
(DN642218) UMC-bof_0A02-021-b03 Ovarian follicle early selec...    46   3.2    1
(DN640643) UMC-bend_0B01-028-g04 Day 16 Uterus from a pregna...    46   3.2    1
(CV981991) UMC-bmix_0A02-008-b03 Day 3 Corpus Luteum bmix Bo...    46   3.2    1
(CV981523) UMC-bmix_0A01-008-b03 Day 3 Corpus Luteum bmix Bo...    46   3.2    1
(EJ635749) 1092989100571 Global-Ocean-Sampling_GS-29-01-01-1...    42   3.6    2
(EJ602295) 1092961205997 Global-Ocean-Sampling_GS-29-01-01-1...    42   4.0    2
(EK579614) 1095521409258 Global-Ocean-Sampling_GS-32-01-01-1...    32   5.8    3
(AC170300) Bos taurus clone CH240-215I20, WORKING DRAFT SEQU...    34   5.9    2
(DE055145) Oryzias latipes DNA, clone: ola1-025N17.F, genomi...    42   8.0    2
(DE356112) Bombyx mori genomic DNA, BAC clone:BET_081_F08.         34   8.1    2
(DE459604) Bombyx mori genomic DNA, Fosmid clone:GBMFno55_h1...    34   8.3    2
(DE565816) Bombyx mori genomic DNA, Fosmid clone:GBMFno208_p...    34   8.4    2
(DE460243) Bombyx mori genomic DNA, Fosmid clone:GBMFno56_f1...    34   8.4    2
(CC486703) CH240_316J15.TARBAC13P2 CHORI-240 Bos taurus geno...    34   10.0   2

>(AU266771) Dictyostelium discoideum vegetative cDNA clone:VSG740,
           5' end single read.
          Length = 469

 Score =  797 bits (402), Expect = 0.0
 Identities = 402/402 (100%)
 Strand = Plus / Plus


Query: 247 gttaaatcgcaacaatcggttacagtactttcagattcaattgaatcatttggaacaatt 306
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 67  gttaaatcgcaacaatcggttacagtactttcagattcaattgaatcatttggaacaatt 126


Query: 307 gaaactgttaaatcaggtgcttcaatttactctttcacattgacctccaaattaccaacc 366
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 127 gaaactgttaaatcaggtgcttcaatttactctttcacattgacctccaaattaccaacc 186


Query: 367 aacattactgtaacaccatgttttggaactttcgatatttatgttagtctaaacaatatt 426
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 187 aacattactgtaacaccatgttttggaactttcgatatttatgttagtctaaacaatatt 246


Query: 427 ataacaaataagagtgcaacctattatggtgaatgggatccatctgtaaaagcacttgga 486
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 247 ataacaaataagagtgcaacctattatggtgaatgggatccatctgtaaaagcacttgga 306


Query: 487 acaaatttaaaagcattacctgaaaatactattatctatattgctgttatgccattagaa 546
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 307 acaaatttaaaagcattacctgaaaatactattatctatattgctgttatgccattagaa 366


Query: 547 gaatatgatttcaaaaacaaaacatatgccgcatccttctctgttttaatcactacaaat 606
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 367 gaatatgatttcaaaaacaaaacatatgccgcatccttctctgttttaatcactacaaat 426


Query: 607 gaacaagatcttgttgttccatatttaccaataccagcaaga 648
           ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 427 gaacaagatcttgttgttccatatttaccaataccagcaaga 468

 Score = 54.0 bits (27), Expect = 0.013
 Identities = 27/27 (100%)
 Strand = Plus / Plus


Query: 660 ttccatatttaccaataccagcaagag 686
           |||||||||||||||||||||||||||
Sbjct: 443 ttccatatttaccaataccagcaagag 469

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 1,098,018,956
Number of extensions: 70577426
Number of successful extensions: 5469023
Number of sequences better than 10.0: 40
Length of query: 1237
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 1213
Effective length of database: 99,340,224,878
Effective search space: 120499692777014
Effective search space used: 120499692777014
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.18
Homology vs Protein 
Query= Contig-U01586-1 (Contig-U01586-1Q) /CSM_Contig/Contig-U01586-1Q.Seq.d
         (1237 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CS272716_1(CS272716|pid:none) Sequence 33 from Patent WO20060129...    37   1.1
CP000263_119(CP000263|pid:none) Buchnera aphidicola str. Cc (Cin...    35   5.5
CP000282_2307(CP000282|pid:none) Saccharophagus degradans 2-40, ...    34   9.5

>CS272716_1(CS272716|pid:none) Sequence 33 from Patent WO2006012904.
          Length = 618

 Score = 37.4 bits (85), Expect = 1.1
 Identities = 30/115 (26%), Positives = 51/115 (44%), Gaps = 3/115 (2%)
 Frame = +1

Query: 292 SFGTIETVKSGASIYSFTLTSKLPTNITVTPCFGTFDIYVSLNNIITNKSATYYGEWDPS 471
           SF   ETV++  S+ S  +T+  P              Y SL++++ + S T +G WDP+
Sbjct: 49  SFTQTETVRTATSVKSAPVTTYAPP-------------YASLSHLVPDLSTTTWGNWDPN 95

Query: 472 VKALGTNLKALPENTIIYIAVMPLEEYDFKNKTYAASFSVLITTN---EQDLVVP 627
             A  T+  A P     + A+   E     N T+   +S  ++       +LV+P
Sbjct: 96  ATATATD-TADPYGQAAWSALW--EHASLANFTFRGLYSTTVSPTPVPTSELVLP 147

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 1,410,174,019
Number of extensions: 25725973
Number of successful extensions: 58015
Number of sequences better than 10.0: 3
Number of HSP's gapped: 57988
Number of HSP's successfully gapped: 3
Length of query: 412
Length of database: 1,051,180,864
Length adjustment: 131
Effective length of query: 281
Effective length of database: 627,191,635
Effective search space: 176240849435
Effective search space used: 176240849435
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.91  gvh: 0.53  alm: 0.21  top: 1.00  tms: 0.20  mit: 0.38  mip: 0.08
 nuc: 0.18  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 32.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for Contig-U01586-1 is pla
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 3
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0