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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U09393-1
Contig ID Contig-U09393-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U09393-1 (Contig-U09393-1Q) /CSM_Contig/Contig-U09393-1Q.Seq.d
AATAATATAAAAAAAAAAAAAAAAAAAAATTAAAAAAAATTAAAGAAACA
AATATTAAAAAAAATAAAAAAAAAATTTAATAATGGGTAAATGGTTAATG
TTAATTGATCCAGCATCATTTTTGATAATAGGGATACCATTATTTGCCAT
ATGGTATGGTAGTTTTAGATCATATAAATTTAATATGGAGAATCAACTTT
TAATAATCGATAACAATGATATGCCAAACAAATGGTTATTAATACTACCT
TGTATTGGTTCATTATCACTCCTCACCTTTTTTTATTATATGGATGCAAT
GTATAATATATTAATTTGTGTTATAGTTATAACATCACTATTAGCCATAT
CTTATGTTACATATCCATTTTTTCAATATTTATTACCAAAGTTTTCAATT
CATGATTCTTCTAAAAAATTAAAAATGTTAGATGATGAAGTTACAATAAC
GTTATCATCTGTAATTGCATTTGTTTGTGGTGTAATATTAACTTTATTTT
GGTATTACTCAAATCATTTTTTATTTGTTAATT----------CATTATT
CGCTGTTTTAATTACAGAGTATGGTCAACCTGCCTTATTATACCTTGTCC
CAACTGTAACTTTACCAACAATTTTCTGTGCTCATAAACGTGGTCATCGA
TCAATTTTAATGAAACCAATACCAAAACCAAAACAAGATCAAGAAAGTTT
AATAAATTCTTGAAAAAAAAAAAAATAAAAAAAAAAAAAAATAGTGATTA
GAA
Gap gap included
Contig length 743
Chromosome number (1..6, M) 5
Chromosome length 5062330
Start point 367271
End point 366522
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 2
Link to clone list U09393
List of clone(s) 

est1=SSG469F,1,534
est2=SSG469Z,535,745
Translated Amino Acid sequence 
ii*kkkkkkikkn*rnky*kk*KKNLIMGKWLMLIDPASFLIIGIPLFAIWYGSFRSYKF
NMENQLLIIDNNDMPNKWLLILPCIGSLSLLTFFYYMDAMYNILICVIVITSLLAISYVT
YPFFQYLLPKFSIHDSSKKLKMLDDEVTITLSSVIAFVCGVILTLFWYYSNHFLFVN---

---LFAVLITEYGQPALLYLVPTVTLPTIFCAHKRGHRSILMKPIPKPKQDQESLINS*k
kkk*kkkkivir
Translated Amino Acid sequence (All Frames) 
Frame A:
nnikkkkkkn*kklkkqilkkikkkfnng*mvnvn*ssiifdnrdtiichmvw*f*ii*i
*ygestfnnr*q*yakqmvinttlywfiitphlfllygcnv*yinlcysynitishilcy
isifsifitkvfns*ff*kiknvr**synnviicnciclwcninfilvllksffic*---

---hyslf*lqsmvnlpyytlsql*lyqqfsvlinvvidqf**nqyqnqnkikkv**ile
kkknkkkkk**le

Frame B:
ii*kkkkkkikkn*rnky*kk*KKNLIMGKWLMLIDPASFLIIGIPLFAIWYGSFRSYKF
NMENQLLIIDNNDMPNKWLLILPCIGSLSLLTFFYYMDAMYNILICVIVITSLLAISYVT
YPFFQYLLPKFSIHDSSKKLKMLDDEVTITLSSVIAFVCGVILTLFWYYSNHFLFVN---

---iircfnyrvwstcliipcpncnftnnflcs*twssinfnetntktktrsrkfnkflk
kkkikkkknsd*

Frame C:
*ykkkkkkklkkiketnikknkkki**wvng*c*liqhhf***gyhylpygmvvldhinl
iwrinf**sitmicqtngy*yylvlvhyhsspffiiwmqciiy*fvl*l*hhy*pylmlh
ihffniyyqsfqfmillkn*kc*mmklq*ryhl*lhlfvv*y*lyfgitqiifylli---

---LFAVLITEYGQPALLYLVPTVTLPTIFCAHKRGHRSILMKPIPKPKQDQESLINS*k
kkk*kkkkivir
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U09393-1 (Contig-U09393-1Q)
/CSM_Contig/Contig-U09393-1Q.Seq.d
         (753 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U09393-1 (Contig-U09393-1Q) /CSM_Contig/Conti...   626  e-180
Contig-U06976-1 (Contig-U06976-1Q) /CSM_Contig/Conti...    38  0.013
Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Conti...    38  0.013
Contig-U14345-1 (Contig-U14345-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U13208-1 (Contig-U13208-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U12288-1 (Contig-U12288-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U12053-1 (Contig-U12053-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U10509-1 (Contig-U10509-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U05468-1 (Contig-U05468-1Q) /CSM_Contig/Conti...    36  0.053
Contig-U07065-1 (Contig-U07065-1Q) /CSM_Contig/Conti...    32  0.83

>Contig-U09393-1 (Contig-U09393-1Q) /CSM_Contig/Contig-U09393-1Q.Seq.d
          Length = 753

 Score =  626 bits (316), Expect = e-180
 Identities = 330/337 (97%)
 Strand = Plus / Plus

                                                                       
Query: 76  tttaataatgggtaaatggttaatgttaattgatccagcatcatttttgataatagggat 135
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 76  tttaataatgggtaaatggttaatgttaattgatccagcatcatttttgataatagggat 135

                                                                       
Query: 136 accattatttgccatatggtatggtagttttagatcatataaatttaatatggagaatca 195
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 136 accattatttgccatatggtatggtagttttagatcatataaatttaatatggagaatca 195

                                                                       
Query: 196 acttttaataatcgataacaatgatatgccaaacaaatggttattaatactaccttgtat 255
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 196 acttttaataatcgataacaatgatatgccaaacaaatggttattaatactaccttgtat 255

                                                                       
Query: 256 tggttcattatcactcctcaccnnnnnnnattatatggatgcaatgtataatatattaat 315
           ||||||||||||||||||||||       |||||||||||||||||||||||||||||||
Sbjct: 256 tggttcattatcactcctcacctttttttattatatggatgcaatgtataatatattaat 315

                                                                       
Query: 316 ttgtgttatagttataacatcactattagccatatcttatgttacatatccattttttca 375
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 316 ttgtgttatagttataacatcactattagccatatcttatgttacatatccattttttca 375

                                                
Query: 376 atatttattaccaaagttttcaattcatgattcttct 412
           |||||||||||||||||||||||||||||||||||||
Sbjct: 376 atatttattaccaaagttttcaattcatgattcttct 412


 Score =  509 bits (257), Expect = e-144
 Identities = 287/287 (100%)
 Strand = Plus / Plus

                                                                       
Query: 426 tgttagatgatgaagttacaataacgttatcatctgtaattgcatttgtttgtggtgtaa 485
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 426 tgttagatgatgaagttacaataacgttatcatctgtaattgcatttgtttgtggtgtaa 485

                                                                       
Query: 486 tattaactttattttggtattactcaaatcattttttatttgttaattnnnnnnnnnnca 545
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 486 tattaactttattttggtattactcaaatcattttttatttgttaattnnnnnnnnnnca 545

                                                                       
Query: 546 ttattcgctgttttaattacagagtatggtcaacctgccttattataccttgtcccaact 605
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 546 ttattcgctgttttaattacagagtatggtcaacctgccttattataccttgtcccaact 605

                                                                       
Query: 606 gtaactttaccaacaattttctgtgctcataaacgtggtcatcgatcaattttaatgaaa 665
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 606 gtaactttaccaacaattttctgtgctcataaacgtggtcatcgatcaattttaatgaaa 665

                                                          
Query: 666 ccaataccaaaaccaaaacaagatcaagaaagtttaataaattcttg 712
           |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 666 ccaataccaaaaccaaaacaagatcaagaaagtttaataaattcttg 712


>Contig-U06976-1 (Contig-U06976-1Q) /CSM_Contig/Contig-U06976-1Q.Seq.d
          Length = 317

 Score = 38.2 bits (19), Expect = 0.013
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 663 aaaccaataccaaaaccaaaaca 685
           ||||||| |||||||||||||||
Sbjct: 131 aaaccaaaaccaaaaccaaaaca 153


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 125 aaaccaaaaccaaaaccaaaac 146


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 95  aaaccaaaaccaaaaccaaaac 116


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 101 aaaccaaaaccaaaaccaaaac 122


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 107 aaaccaaaaccaaaaccaaaac 128


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 113 aaaccaaaaccaaaaccaaaac 134


 Score = 36.2 bits (18), Expect = 0.053
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 663 aaaccaataccaaaaccaaaac 684
           ||||||| ||||||||||||||
Sbjct: 119 aaaccaaaaccaaaaccaaaac 140


>Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Contig-U05086-1Q.Seq.d
          Length = 1025

 Score = 38.2 bits (19), Expect = 0.013
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 671 accaaaaccaaaacaagat 689
           |||||||||||||||||||
Sbjct: 195 accaaaaccaaaacaagat 213


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 10,780
Number of Sequences: 6905
Number of extensions: 10780
Number of successful extensions: 1188
Number of sequences better than 10.0: 134
length of query: 753
length of database: 5,674,871
effective HSP length: 16
effective length of query: 737
effective length of database: 5,564,391
effective search space: 4100956167
effective search space used: 4100956167
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.15
Homology vs DNA 
Query= Contig-U09393-1 (Contig-U09393-1Q) /CSM_Contig/Contig-U09393-1Q.Seq.d
         (753 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C94152) Dictyostelium discoideum slug cDNA, clone SSG469.        335   1e-87  1
(AU073241) Dictyostelium discoideum slug cDNA, clone SSG469.      327   3e-85  1
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...    40   0.040  7
(CR352232) Zebrafish DNA sequence from clone DKEY-3L8 in lin...    50   0.12   1
(AC111443) Rattus norvegicus clone CH230-192H9, WORKING DRAF...    50   0.12   1
(AC161498) Mus musculus chromosome 1 clone RP24-249L3 map 1,...    42   0.18   3
(AL807392) Mouse DNA sequence from clone RP23-104D24 on chro...    48   0.48   1
(AL731790) Mouse DNA sequence from clone RP23-104P2 on chrom...    48   0.48   1
(AC074337) Mus musculus strain C57BL6/J chromosome 2 clone R...    48   0.48   1
(AC185025) Bos taurus clone CH240-271C3, WORKING DRAFT SEQUE...    48   0.48   1
(AC172151) Bos taurus clone CH240-251M4, WORKING DRAFT SEQUE...    48   0.48   1
(AC170830) Bos taurus clone CH240-251M23, WORKING DRAFT SEQU...    48   0.48   1
(BH044906) RPCI-24-375K18.TJ RPCI-24 Mus musculus genomic cl...    48   0.48   1
(AZ498071) 1M0335K10F Mouse 10kb plasmid UUGC1M library Mus ...    48   0.48   1
(ER332453) 1092344275207 Global-Ocean-Sampling_GS-34-01-01-1...    36   1.2    2
(EJ791836) 1093017381100 Global-Ocean-Sampling_GS-30-02-01-1...    36   1.4    2
(AC015459) Homo sapiens clone RP11-115G11, LOW-PASS SEQUENCE...    40   1.5    4
(ER487951) 1093015309499 Global-Ocean-Sampling_GS-35-01-01-1...    44   1.5    2
(AC175048) Medicago truncatula chromosome 2 BAC clone mth2-7...    38   1.5    5
(CR855360) Zebrafish DNA sequence from clone DKEY-178C13 in ...    46   1.9    1
(AL954312) Zebrafish DNA sequence from clone CH211-220P9 in ...    46   1.9    1
(AC163297) Mus musculus BAC clone RP23-106G24 from chromosom...    46   1.9    1
(AC152159) Mus musculus chromosome 15, clone RP23-10O8, comp...    46   1.9    1
(AC122219) Mus musculus BAC clone RP23-119F18 from 3, comple...    46   1.9    1
(CU915286) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   1.9    1
(CU861578) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.9    1
(CU633672) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.9    1
(CU467736) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   1.9    1
(CU467650) Zebrafish DNA sequence *** SEQUENCING CANCELLED *...    46   1.9    1
(CR854852) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   1.9    1
(ED566397) AUAC-aaz52h03.b1 Ascaris suum whole genome shotgu...    46   1.9    1
(CK947222) 4071830 BARC 10BOV Bos taurus cDNA clone 10BOV1_B...    46   1.9    1
(CK002446) AGENCOURT_16385473 NIH_MGC_227 Homo sapiens cDNA ...    46   1.9    1
(AL356858) Human DNA sequence from clone RP11-357K9 on chrom...    40   1.9    4
(BP136701) Nicotiana tabacum cDNA, clone: BY9314, primer: M1...    36   3.0    2
(AC131072) Mus musculus chromosome 1, clone RP23-396F21, com...    42   3.4    2
(AL741951) Danio rerio genomic clone DKEY-22G4, genomic surv...    40   3.5    2
(AC026337) Homo sapiens 2 BAC RP11-105E24 (Roswell Park Canc...    32   3.7    2
(AC018678) Homo sapiens BAC clone RP11-279E17 from 2, comple...    32   3.7    2
(AC152871) Medicago truncatula clone mth2-83e5, complete seq...    42   3.7    4
(AC189552) Brassica rapa subsp. pekinensis clone KBrH005P10,...    38   3.7    5
(AC095614) Rattus norvegicus clone CH230-8C15, *** SEQUENCIN...    40   4.7    3
(ER411899) 1095375004220 Global-Ocean-Sampling_GS-34-01-01-1...    32   4.9    2
(CX117998) EI112I11 A normalized whole-life-cycle cDNA libra...    40   5.0    2
(EJ224500) 1092351355253 Global-Ocean-Sampling_GS-27-01-01-1...    40   5.0    2
(EJ168013) 1092344072910 Global-Ocean-Sampling_GS-27-01-01-1...    40   5.3    2
(AE017243) Mycoplasma hyopneumoniae J, complete genome.            44   5.4    10
(AC151425) Medicago truncatula chromosome 8 clone mth2-4l6, ...    42   5.4    4
(BM807491) AGENCOURT_6581883 NIH_MGC_41 Homo sapiens cDNA cl...    36   5.6    3
(U23511) Caenorhabditis elegans cosmid C32D5, complete seque...    36   5.9    4
(AC004688) Plasmodium falciparum chromosome 12 clone 3D7, **...    32   6.5    8
(AJ969242) SVTS2 plectrovirus, complete genome.                    38   7.1    3
(AC168553) Strongylocentrotus purpuratus clone R3-3091G11, W...    44   7.3    3
(DE643444) Bombyx mori genomic DNA, Fosmid clone:GBMFno392_n...    36   7.3    2
(CD451310) USDA-FP_103416 Adult Alate Brown Citrus Aphid Tox...    34   7.4    3
(CU469339) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    42   7.4    4
(BX000438) Zebrafish DNA sequence from clone CH211-209M20 in...    44   7.5    1
(AL645743) Zebrafish DNA sequence from clone RP71-1N7 in lin...    44   7.5    1
(AL672047) Mouse DNA sequence from clone RP23-316B4 on chrom...    44   7.5    1
(AC134867) Mus musculus BAC clone RP23-18L24 from 10, comple...    44   7.5    1
(AC130711) Mus musculus BAC clone RP23-22M24 from chromosome...    44   7.5    1
(AC064793) Mus musculus strain C57BL6/J chromosome 5 clone R...    44   7.5    1
(AC027653) Mus musculus strain C57BL6/J chromosome 5 clone R...    44   7.5    1
(AM438758) Vitis vinifera contig VV78X225009.9, whole genome...    44   7.5    1
(AL354861) Human DNA sequence from clone RP11-180I4 on chrom...    44   7.5    1
(AC130004) Homo sapiens 3 BAC RP11-237K2 (Roswell Park Cance...    44   7.5    1
(AC117515) Homo sapiens 3 BAC RP11-797J6 (Roswell Park Cance...    44   7.5    1
(AC159727) Bos taurus clone CH240-73D13, WORKING DRAFT SEQUE...    44   7.5    1
(AC135301) Rattus norvegicus clone CH230-45E14, *** SEQUENCI...    44   7.5    1
(AC132531) Rattus norvegicus clone CH230-212C6, *** SEQUENCI...    44   7.5    1
(AC125916) Rattus norvegicus clone CH230-193L7, *** SEQUENCI...    44   7.5    1
(AC115376) Rattus norvegicus clone CH230-38C15, *** SEQUENCI...    44   7.5    1
(AC107547) Rattus norvegicus clone CH230-38L23, *** SEQUENCI...    44   7.5    1
(CU856296) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   7.5    1
(AC093976) Rattus norvegicus clone CH230-59P13, WORKING DRAF...    44   7.5    1
(AC093354) Mus musculus clone RP23-20D11, WORKING DRAFT SEQU...    44   7.5    1
(AC091323) Mus musculus chromosome 17 clone RP23-333E5 map 1...    44   7.5    1
(CU466546) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   7.5    1
(BX936294) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    44   7.5    1
(BX248126) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    44   7.5    1
(AC207515) Spermophilus tridecemlineatus clone VMRC20-212N6,...    44   7.5    1
(AC198579) Spermophilus tridecemlineatus clone VMRC20-604D19...    44   7.5    1
(AC172917) Bos taurus clone CH240-308J2, WORKING DRAFT SEQUE...    44   7.5    1
(AC163781) Bos taurus clone CH240-148M4, WORKING DRAFT SEQUE...    44   7.5    1
(AC011845) Homo sapiens chromosome 9 clone RP11-311O2 map 9,...    44   7.5    1
(AK036995) Mus musculus adult female vagina cDNA, RIKEN full...    44   7.5    1
(AQ615679) HS_5145_B2_G01_SP6E RPCI-11 Human Male BAC Librar...    44   7.5    1
(EK436192) 1095521046494 Global-Ocean-Sampling_GS-31-01-01-1...    44   7.5    1
(DU853670) 57378 Tomato HindIII BAC Library Solanum lycopers...    44   7.5    1
(DU035911) 18302 Tomato HindIII BAC Library Solanum lycopers...    44   7.5    1
(CW932296) EDCCC59TR A. castellanii, 6-8 kb library from tot...    44   7.5    1
(BX168652) Danio rerio genomic clone DKEY-179J6, genomic sur...    44   7.5    1
(DW514565) GH_TMIRS_183_E09_F Cotton Normalized Library dT p...    44   7.5    1
(DW505193) GH_TMIRS_091_F04_F Cotton Normalized Library dT p...    44   7.5    1
(DB878666) Populus nigra mRNA, clone: PnFL2-023_J24, 5'end.        44   7.5    1
(DQ386466) Mycoplasma hyopneumoniae strain PMS VNTAR-contain...    44   7.5    1
(CP001047) Mycoplasma arthritidis 158L3-1, complete genome.        44   7.5    1
(AC231674) Solanum tuberosum chromosome 11 clone RH195J04, c...    36   8.0    4
(AC115594) Dictyostelium discoideum chromosome 2 map 4071862...    42   8.6    2
(AL773528) Human DNA sequence *** SEQUENCING IN PROGRESS ***...    36   8.7    4

>(C94152) Dictyostelium discoideum slug cDNA, clone SSG469.
          Length = 211

 Score =  335 bits (169), Expect = 1e-87
 Identities = 169/169 (100%)
 Strand = Plus / Plus


Query: 544 cattattcgctgttttaattacagagtatggtcaacctgccttattataccttgtcccaa 603
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   cattattcgctgttttaattacagagtatggtcaacctgccttattataccttgtcccaa 60


Query: 604 ctgtaactttaccaacaattttctgtgctcataaacgtggtcatcgatcaattttaatga 663
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  ctgtaactttaccaacaattttctgtgctcataaacgtggtcatcgatcaattttaatga 120


Query: 664 aaccaataccaaaaccaaaacaagatcaagaaagtttaataaattcttg 712
           |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aaccaataccaaaaccaaaacaagatcaagaaagtttaataaattcttg 169

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 771,159,667
Number of extensions: 47615343
Number of successful extensions: 4002803
Number of sequences better than 10.0: 102
Length of query: 753
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 729
Effective length of database: 97,308,875,965
Effective search space: 70938170578485
Effective search space used: 70938170578485
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.25
Homology vs Protein 
Query= Contig-U09393-1 (Contig-U09393-1Q) /CSM_Contig/Contig-U09393-1Q.Seq.d
         (753 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK223590_1(AK223590|pid:none) Homo sapiens mRNA for SPPL3 protei...    51   3e-05
BC086646_1(BC086646|pid:none) Mus musculus signal peptide peptid...    51   3e-05
AK014709_1(AK014709|pid:none) Mus musculus 0 day neonate head cD...    51   3e-05
(Q9CUS9) RecName: Full=Signal peptide peptidase-like 3;         ...    51   3e-05
AK291161_1(AK291161|pid:none) Homo sapiens cDNA FLJ75194 complet...    49   2e-04
(Q8TCT6) RecName: Full=Signal peptide peptidase-like 3;         ...    47   5e-04
BT085527_1(BT085527|pid:none) Zea mays full-length cDNA clone ZM...    44   0.005
BC097153_1(BC097153|pid:none) Danio rerio signal peptide peptida...    44   0.005
BC061375_1(BC061375|pid:none) Xenopus tropicalis signal peptide ...    42   0.027
AK074916_1(AK074916|pid:none) Homo sapiens cDNA FLJ90435 fis, cl...    41   0.046
AM465880_1(AM465880|pid:none) Vitis vinifera contig VV78X096674....    39   0.13
AY058181_1(AY058181|pid:none) Arabidopsis thaliana AT4g33410/F17...    39   0.17
AE014297_4031(AE014297|pid:none) Drosophila melanogaster chromos...    38   0.30
BT003288_1(BT003288|pid:none) Drosophila melanogaster GM06145 fu...    38   0.30
AJ493124_8(AJ493124|pid:none) Varroa destructor complete mitocho...    37   0.66
AY163547_8(AY163547|pid:none) Varroa destructor mitochondrion, c...    37   0.66
BA000028_402(BA000028|pid:none) Oceanobacillus iheyensis HTE831 ...    36   1.5
CP000678_982(CP000678|pid:none) Methanobrevibacter smithii ATCC ...    35   1.9
Z49067_4(Z49067|pid:none) Caenorhabditis elegans Cosmid C44F1, c...    35   3.3
AP008971_1337(AP008971|pid:none) Finegoldia magna ATCC 29328 DNA...    35   3.3
AF300563_2(AF300563|pid:none) Diadasia sphaeralcearum affinis sp...    35   3.3
EU255915_4(EU255915|pid:none) Lactococcus lactis subsp. lactis c...    35   3.3
BA000026_283(BA000026|pid:none) Mycoplasma penetrans HF-2 DNA, c...    35   3.3
AL031632_3(AL031632|pid:none) Caenorhabditis elegans YAC Y32B12B...    35   3.3
CP000388_2521(CP000388|pid:none) Pseudoalteromonas atlantica T6c...    34   5.6
AC092388_28(AC092388|pid:none) Oryza sativa chromosome 10 BAC OS...    34   5.6
AM398681_292(AM398681|pid:none) Flavobacterium psychrophilum JIP...    34   5.6
EU746612_8(EU746612|pid:none) Nasonia longicornis strain IV7 NAD...    34   5.6
(Q05FH9) RecName: Full=DNA-directed RNA polymerase subunit beta'...    33   9.6
(P33033) RecName: Full=Melanocortin receptor 3; AltName: Full=MC...    33   9.6
CP000388_261(CP000388|pid:none) Pseudoalteromonas atlantica T6c,...    33   9.6

>AK223590_1(AK223590|pid:none) Homo sapiens mRNA for SPPL3 protein
           variant, clone: FCC130F12.
          Length = 384

 Score = 51.2 bits (121), Expect = 3e-05
 Identities = 38/160 (23%), Positives = 66/160 (41%), Gaps = 16/160 (10%)
 Frame = +2

Query: 101 LIDPASFLIIGIPLFAIWYGSFRSYKFNMENQLLIIDNNDMPNKW--------------- 235
           L+D +      I +  I YGSFRS   + ENQ    D+N     +
Sbjct: 12  LVDSSQVSTFLISILLIVYGSFRSLNMDFENQDKEKDSNSSSGSFNGNSTNNSIQTIDST 71

Query: 236 -LLILPCIGSLSLLTFFYYMDAMYNILICVIVITSLLAISYVTYPFFQYLLPKFSIHDSS 412
             L LP   S+SLL  F++ D++  +      + + +A +++  P  QYL    S  +
Sbjct: 72  QTLFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKI 131

Query: 413 KKLKMLDDEVTITLSSVIAFVCGVILTLFWYYSNHFLFVN 532
                       T + +++F   V+L L W  + H+L ++
Sbjct: 132 S----FGCCGRFTAAELLSFSLSVMLVLIWVLTGHWLLMD 167

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 966,410,638
Number of extensions: 17274099
Number of successful extensions: 41815
Number of sequences better than 10.0: 31
Number of HSP's gapped: 41775
Number of HSP's successfully gapped: 32
Length of query: 251
Length of database: 1,040,966,779
Length adjustment: 125
Effective length of query: 126
Effective length of database: 640,431,154
Effective search space: 80694325404
Effective search space used: 80694325404
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.82  gvh: 0.58  alm: 0.17  top: 0.33  tms: 0.33  mit: 0.21  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 20.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U09393-1 is end
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0