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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U09386-1
Contig ID Contig-U09386-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U09386-1 (Contig-U09386-1Q) /CSM_Contig/Contig-U09386-1Q.Seq.d
AAATTCAACACCACAACAAAAAAAAAATTAAATTCTAAAAATATAACACA
TTAAAAAATTTATTTTTATATATATAAATTATGGCAAATTCAAAATATGA
ATATGTTAAATCATTTGAACAACCAGATATATTATTACAAAATGTTTGGA
TTGTAGTACGTATTGATGGTAGATCATTTCACAAATTTACAACTAAACAT
GATTATGCAAAACCAAATGATGATAGAGGTTTATCATTAATGAATAGAGC
CGCTCTTGAAGTTTGTAAAGAATTTCCTGATATCGTTATAGCATTTGGTG
AAAGTGATGAATATAGTTTTGTTTTAAAAAAGAGTTGTAATTTATTTGAA
AGAAGATCTAGTAAAATTTCATCATCAATTGTATCATATTTTACAAGCCA
ATTTGTTTATAGATGGAAGGAATATTTTGGAGAACATGAATTAAAATATC
CTCCAACTTTTGATTCAAGATGTGTTCTTTATCCAACTGATGAAAATATA
AAAGATTATCTTAGTTGGAGACAAGCAGATACACATATAAA---------
-TTTAATATAAATTATAATAATTTACCAGCAGAATATAGGAAGGGTTCTG
TTATCTTTAAGAAACCAGTACAAGAAACTAATAAAGAAACTGGCTTAACA
AAATCAAAAAAGGGATTAGTTATTGAACATGTTGACATTATTTCTGAAAA
ATTTTGGAAAGAATACCCTGATATTTTAGCTTCAAAATAAAATAAAAAAA
AAATGGAACTCCATTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA
Gap gap included
Contig length 812
Chromosome number (1..6, M) 6
Chromosome length 3595308
Start point 821563
End point 822378
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 2
Link to clone list U09386
List of clone(s) 

est1=SSF801F,1,542
est2=SSF801Z,543,814
Translated Amino Acid sequence 
iqhhnkkkikf*kyntlknlflyi*IMANSKYEYVKSFEQPDILLQNVWIVVRIDGRSFH
KFTTKHDYAKPNDDRGLSLMNRAALEVCKEFPDIVIAFGESDEYSFVLKKSCNLFERRSS
KISSSIVSYFTSQFVYRWKEYFGEHELKYPPTFDSRCVLYPTDENIKDYLSWRQADTHI-
--

---FNINYNNLPAEYRKGSVIFKKPVQETNKETGLTKSKKGLVIEHVDIISEKFWKEYPD
ILASK*nkkkmelhffkkkkkkkkkkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
kfntttkkklnsknith*kiyfyiyklwqiqnmnmlnhlnnqiyyykmfgl*yvlmvdhf
tnlqlnmimqnqmmievyh**iepllkfvknflisl*hlvkvmnivlf*krvviylkedl
vkfhhqlyhilqanlfidgrnilenmn*nilqlliqdvffiqlmki*kiilvgdkqihi*
---

---FNINYNNLPAEYRKGSVIFKKPVQETNKETGLTKSKKGLVIEHVDIISEKFWKEYPD
ILASK*nkkkmelhffkkkkkkkkkkkkkkkkk

Frame B:
nstpqqkkn*ilki*hikkfifiyinygkfki*ic*ii*ttryiitkcldcsty*w*iis
qiyn*t*lcktk***rfiine*srs*sl*ris*yrysiw*k**i*fcfkkel*fi*kki*
*nfiinciifykpicl*megifwrt*ikissnf*fkmcslsn**kykrls*letsrytyk
---

---li*iiiiyqqnigrvllslrnqykklikkla*qnqkrd*llnmltlflknfgkntli
f*lqnkikkkwnsiflkkkkkkkkkkkkkkkkk

Frame C:
iqhhnkkkikf*kyntlknlflyi*IMANSKYEYVKSFEQPDILLQNVWIVVRIDGRSFH
KFTTKHDYAKPNDDRGLSLMNRAALEVCKEFPDIVIAFGESDEYSFVLKKSCNLFERRSS
KISSSIVSYFTSQFVYRWKEYFGEHELKYPPTFDSRCVLYPTDENIKDYLSWRQADTHI-
--

---*ykl**ftsri*egfcyl*etstrn**rnwlnkikkgisy*tc*hyf*kilerip*y
fsfkik*kkngtpff*kkkkkkkkkkkkkkkk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U09386-1 (Contig-U09386-1Q)
/CSM_Contig/Contig-U09386-1Q.Seq.d
         (822 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U09386-1 (Contig-U09386-1Q) /CSM_Contig/Conti...  1231  0.0
Contig-U03923-1 (Contig-U03923-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U09404-1 (Contig-U09404-1Q) /CSM_Contig/Conti...    38  0.015
Contig-U11741-1 (Contig-U11741-1Q) /CSM_Contig/Conti...    36  0.058
Contig-U09649-1 (Contig-U09649-1Q) /CSM_Contig/Conti...    36  0.058
Contig-U14498-1 (Contig-U14498-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U14428-1 (Contig-U14428-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U14271-1 (Contig-U14271-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U13909-1 (Contig-U13909-1Q) /CSM_Contig/Conti...    34  0.23
Contig-U12453-1 (Contig-U12453-1Q) /CSM_Contig/Conti...    34  0.23

>Contig-U09386-1 (Contig-U09386-1Q) /CSM_Contig/Contig-U09386-1Q.Seq.d
          Length = 822

 Score = 1231 bits (621), Expect = 0.0
 Identities = 651/651 (100%)
 Strand = Plus / Plus

                                                                       
Query: 83  ggcaaattcaaaatatgaatatgttaaatcatttgaacaaccagatatattattacaaaa 142
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 83  ggcaaattcaaaatatgaatatgttaaatcatttgaacaaccagatatattattacaaaa 142

                                                                       
Query: 143 tgtttggattgtagtacgtattgatggtagatcatttcacaaatttacaactaaacatga 202
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 143 tgtttggattgtagtacgtattgatggtagatcatttcacaaatttacaactaaacatga 202

                                                                       
Query: 203 ttatgcaaaaccaaatgatgatagaggtttatcattaatgaatagagccgctcttgaagt 262
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 203 ttatgcaaaaccaaatgatgatagaggtttatcattaatgaatagagccgctcttgaagt 262

                                                                       
Query: 263 ttgtaaagaatttcctgatatcgttatagcatttggtgaaagtgatgaatatagttttgt 322
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 263 ttgtaaagaatttcctgatatcgttatagcatttggtgaaagtgatgaatatagttttgt 322

                                                                       
Query: 323 tttaaaaaagagttgtaatttatttgaaagaagatctagtaaaatttcatcatcaattgt 382
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 323 tttaaaaaagagttgtaatttatttgaaagaagatctagtaaaatttcatcatcaattgt 382

                                                                       
Query: 383 atcatattttacaagccaatttgtttatagatggaaggaatattttggagaacatgaatt 442
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 383 atcatattttacaagccaatttgtttatagatggaaggaatattttggagaacatgaatt 442

                                                                       
Query: 443 aaaatatcctccaacttttgattcaagatgtgttctttatccaactgatgaaaatataaa 502
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 443 aaaatatcctccaacttttgattcaagatgtgttctttatccaactgatgaaaatataaa 502

                                                                       
Query: 503 agattatcttagttggagacaagcagatacacatataaannnnnnnnnntttaatataaa 562
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 503 agattatcttagttggagacaagcagatacacatataaannnnnnnnnntttaatataaa 562

                                                                       
Query: 563 ttataataatttaccagcagaatataggaagggttctgttatctttaagaaaccagtaca 622
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 563 ttataataatttaccagcagaatataggaagggttctgttatctttaagaaaccagtaca 622

                                                                       
Query: 623 agaaactaataaagaaactggcttaacaaaatcaaaaaagggattagttattgaacatgt 682
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 623 agaaactaataaagaaactggcttaacaaaatcaaaaaagggattagttattgaacatgt 682

                                                              
Query: 683 tgacattatttctgaaaaattttggaaagaataccctgatattttagcttc 733
           |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 683 tgacattatttctgaaaaattttggaaagaataccctgatattttagcttc 733


 Score = 34.2 bits (17), Expect = 0.23
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                           
Query: 1  aaattcaacaccacaac 17
          |||||||||||||||||
Sbjct: 1  aaattcaacaccacaac 17


 Score = 34.2 bits (17), Expect = 0.23
 Identities = 17/17 (100%)
 Strand = Plus / Plus

                            
Query: 754 tggaactccattttttt 770
           |||||||||||||||||
Sbjct: 754 tggaactccattttttt 770


>Contig-U03923-1 (Contig-U03923-1Q) /CSM_Contig/Contig-U03923-1Q.Seq.d
          Length = 1770

 Score = 40.1 bits (20), Expect = 0.004
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 556 atataaattataataattta 575
           ||||||||||||||||||||
Sbjct: 289 atataaattataataattta 270


>Contig-U09404-1 (Contig-U09404-1Q) /CSM_Contig/Contig-U09404-1Q.Seq.d
          Length = 1014

 Score = 38.2 bits (19), Expect = 0.015
 Identities = 25/27 (92%)
 Strand = Plus / Plus

                                      
Query: 93  aaatatgaatatgttaaatcatttgaa 119
           ||||||||||||| ||||||| |||||
Sbjct: 718 aaatatgaatatggtaaatcaattgaa 744


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 12,757
Number of Sequences: 6905
Number of extensions: 12757
Number of successful extensions: 1427
Number of sequences better than 10.0: 166
length of query: 822
length of database: 5,674,871
effective HSP length: 16
effective length of query: 806
effective length of database: 5,564,391
effective search space: 4484899146
effective search space used: 4484899146
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.15
Homology vs DNA 
Query= Contig-U09386-1 (Contig-U09386-1Q) /CSM_Contig/Contig-U09386-1Q.Seq.d
         (822 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C84782) Dictyostelium discoideum slug cDNA, clone SSF801.        361   3e-95  1
(AU073050) Dictyostelium discoideum slug cDNA, clone SSF801.      256   2e-66  2
(FC766618) CBBN3253.fwd CBBN Lottia gigantea 3,4,5,6.5d Larv...    46   3e-07  3
(FC733552) CBBG7986.fwd CBBG Lottia gigantea 12,15,18h embry...    46   4e-07  3
(FC802812) CBGC536.fwd CBGC Lottia gigantea 15h 18h embryos ...    46   5e-07  3
(EX609030) 255368529 Pea aphid whole body normalized full le...    52   2e-06  2
(FC802811) CBGC536.rev CBGC Lottia gigantea 15h 18h embryos ...    46   6e-06  3
(EX636879) 256611920 Pea aphid whole body normalized full le...    52   8e-06  2
(EX636130) 256606640 Pea aphid whole body normalized full le...    52   8e-06  2
(FF330634) 280443228 Pea aphid whole body normalized full le...    52   9e-06  2
(FF331528) 280446876 Pea aphid whole body normalized full le...    52   9e-06  2
(AL844506) Plasmodium falciparum chromosome  7.                    56   9e-05  12
(EX608113) 255361175 Pea aphid whole body normalized full le...    44   0.002  2
(FF326576) 280430253 Pea aphid whole body normalized full le...    52   0.006  2
(EC390402) C12_C12nb2j23_pDNR-f_449312 Myzus persicae, tobac...    34   0.010  4
(BU465388) 603775513F1 CSEQRBN19 Gallus gallus cDNA clone Ch...    46   0.024  2
(AJ450063) Gallus gallus EST, clone library riken1, clone 24...    46   0.025  2
(BW535244) Ciona savignyi cDNA, clone:csma084j19, 5'end, sin...    34   0.025  3
(CF251573) hdm001_g04 LPS-activated macrophage cell line Gal...    46   0.030  2
(DR411222) gPGC_EST01064 Embryonic gonadal PGC cDNA Library ...    46   0.044  2
(DR411226) gPGC_EST01068 Embryonic gonadal PGC cDNA Library ...    46   0.045  2
(DR415360) gPGC_EST05202 Embryonic gonadal PGC cDNA Library ...    46   0.046  2
(AJ720720) Gallus gallus mRNA for hypothetical protein, clon...    46   0.051  2
(CJ392787) Molgula tectiformis cDNA, gonad clone:mtgd007i12,...    46   0.079  2
(CJ354105) Molgula tectiformis cDNA, cleaving embryo clone:m...    46   0.093  2
(CJ415104) Molgula tectiformis cDNA, larva clone:mtlv020j15,...    46   0.098  2
(CJ424293) Molgula tectiformis cDNA, larva clone:mtlv020j15,...    46   0.11   2
(CJ403410) Molgula tectiformis cDNA, gonad clone:mtgd007i12,...    46   0.11   2
(CJ364569) Molgula tectiformis cDNA, cleaving embryo clone:m...    46   0.11   2
(DV619989) EST1222985 Glossina morsitans morsitans Fat body ...    38   0.40   2
(AC186278) Spermophilus tridecemlineatus clone VMRC20-243P12...    42   0.44   5
(EC825719) SME00003353 esmbsro2 Sawyeria marylandensis cDNA,...    34   0.48   3
(EC825493) SME00008324 esmbsro2 Sawyeria marylandensis cDNA,...    34   0.50   3
(DX520035) GH_MBb0042A08r GH_MBb Gossypium hirsutum genomic ...    48   0.53   1
(FF294863) 279303623 Pea aphid whole body normalized full le...    48   0.53   1
(EX623959) 255426877 Pea aphid whole body normalized full le...    48   0.53   1
(CN629103) taf46e04.y1 Hydra EST Darmstadt I Hydra magnipapi...    42   0.97   2
(AB231328) Plasmodium berghei lsp2 gene for liver stage prot...    40   1.1    2
(AC184184) Strongylocentrotus purpuratus clone R3-3021D6, WO...    46   1.1    3
(DN243534) ACAE-aaa36j07.g1 Hydra EST UCI 5 Hydra magnipapil...    42   1.2    2
(EX857897) CBNF4222.rev CBNF Phycomyces blakesleeanus NRRL15...    38   1.2    2
(EX857898) CBNF4222.fwd CBNF Phycomyces blakesleeanus NRRL15...    38   1.2    2
(DQ274179) Solanum tuberosum patatin-rich BAC 14K07, complet...    34   1.9    6
(Z98547) Plasmodium falciparum MAL3P3.                             38   2.1    4
(AJ507411) Elaeis guineensis partial pol gene for reverse tr...    46   2.1    1
(AJ507409) Elaeis guineensis partial pol gene for reverse tr...    46   2.1    1
(AJ507408) Elaeis guineensis partial pol gene for reverse tr...    46   2.1    1
(U97407) Caenorhabditis elegans cosmid C34G6, complete seque...    46   2.1    1
(FI134055) CsB10STC_04C22R_864 ends of BAC library of B10 li...    46   2.1    1
(FH045501) CHO_OF3194xp18r1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   2.1    1
(AJ722425) Gallus gallus EST, clone 24a8s3.                        46   2.1    1
(FH701336) CHO_OF5159xk20f1.ab1 CHO_OF5 Nicotiana tabacum ge...    36   2.1    3
(AC173907) Microcebus murinus clone CH257-213E6, WORKING DRA...    42   2.5    5
(CP000770) Candidatus Sulcia muelleri GWSS, complete genome.       36   3.2    8
(AX652579) Sequence 2449 from Patent WO03000898.                   36   3.4    2
(AX509492) Sequence 4187 from Patent WO0216655.                    36   3.4    2
(AC115366) Rattus norvegicus clone CH230-59K8, *** SEQUENCIN...    34   4.1    7
(CN628899) taf46e04.x1 Hydra EST Darmstadt I Hydra magnipapi...    42   4.5    2
(CX023939) Mdlv4-4049a13.y1 Mdlv4 Malus x domestica cDNA clo...    36   4.8    2
(EC334070) GUTF089509K2 POSSUM_01-POSSUM-GUT-2KB Trichosurus...    36   4.8    2
(EC303055) OVARYF088534K23 POSSUM_01-POSSUM-C-OVARY-2KB Tric...    36   4.9    2
(DY608031) IMMUNEF045813I1 POSSUM_01-C-POSSUM-IMMUNE-2KB Tri...    36   5.0    2
(FG875115) UCRVU07_CCNP10522_g1 Cowpea UCR 779 Mixed Tissue ...    36   5.0    2
(EC308828) OVARYF089642D5 POSSUM_01-POSSUM-C-OVARY-2KB Trich...    36   5.0    2
(DY591185) GUTF074985F24 POSSUM_01-POSSUM-GUT-2KB Trichosuru...    36   5.1    2
(DY603797) LIVERF031286C10 POSSUM_01-POSSUM-LIVER-2KB Tricho...    36   5.2    2
(EC308220) OVARYF091729K5 POSSUM_01-POSSUM-C-OVARY-2KB Trich...    36   5.3    2
(DY612143) IMMUNEF045808O17 POSSUM_01-C-POSSUM-IMMUNE-2KB Tr...    36   5.4    2
(EJ648573) 1092953002618 Global-Ocean-Sampling_GS-30-02-01-1...    38   5.5    2
(AP008971) Finegoldia magna ATCC 29328 DNA, complete genome.       34   5.5    14
(EC348946) LIVERF089506J4 POSSUM_01-POSSUM-LIVER-2KB Trichos...    36   5.6    2
(EC341839) KIDNEYF089708G3 POSSUM_01-POSSUM-KIDNEY-2KB Trich...    36   5.7    2
(EC289941) BRAINF089693C11 POSSUM_01-POSSUM-C-BRAIN-2KB Tric...    36   5.7    2
(DY611283) IMMUNEF045817O13 POSSUM_01-C-POSSUM-IMMUNE-2KB Tr...    36   5.7    2
(AC019133) Homo sapiens BAC clone RP11-577G20 from 4, comple...    38   5.9    5
(EJ709254) 1092956067299 Global-Ocean-Sampling_GS-30-02-01-1...    38   6.0    2
(AC144642) Canis familiaris clone RP81-352C6, WORKING DRAFT ...    36   6.7    6
(AC153271) Bos taurus clone CH240-20N1, WORKING DRAFT SEQUEN...    40   7.2    4
(ER535806) 1093015839236 Global-Ocean-Sampling_GS-35-01-01-1...    34   7.3    3
(CU326389) Medicago truncatula chromosome 5 clone mth2-179e2...    38   7.5    4
(AL592306) Zebrafish DNA sequence from clone BUSM1-67D8 in l...    36   7.5    4
(CU499387) Zebrafish DNA sequence from clone CH73-30N17 in l...    44   8.2    1
(CR936322) Zebrafish DNA sequence from clone DKEY-91I17 in l...    44   8.2    1
(CR925821) Zebrafish DNA sequence from clone DKEY-27D1 in li...    44   8.2    1
(CR735130) Zebrafish DNA sequence from clone DKEY-206F21 in ...    44   8.2    1
(BX470199) Zebrafish DNA sequence from clone CH211-226M7 in ...    44   8.2    1
(BX324111) Zebrafish DNA sequence from clone CH211-119C17 in...    44   8.2    1
(BX323799) Zebrafish DNA sequence from clone CH211-159B19 in...    44   8.2    1
(AL954313) Zebrafish DNA sequence from clone CH211-257J20 in...    44   8.2    1
(AC188078) Macaca mulatta chromosome UNK clone CH250-50H12, ...    44   8.2    1
(AF001339) Hydnophytum formicarum ribosomal protein S16 (rps...    44   8.2    1
(EF419161) Spartaeus jianfengensis NADH dehydrogenase subuni...    44   8.2    1
(AY449462) Oikopleura dioica clone BACOIKO006 3xn11, complet...    44   8.2    1
(AC126750) Homo sapiens chromosome 5 clone CTD-2055H2, compl...    44   8.2    1
(AC122715) Homo sapiens chromosome 5 clone RP11-52C13, compl...    44   8.2    1
(AC004063) Homo sapiens chromosome 4 clone B32I8, complete s...    44   8.2    1
(AC111233) Rattus norvegicus clone CH230-94N11, *** SEQUENCI...    44   8.2    1
(CU861934) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   8.2    1
(CU467878) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    44   8.2    1
(CR559928) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    44   8.2    1

>(C84782) Dictyostelium discoideum slug cDNA, clone SSF801.
          Length = 272

 Score =  361 bits (182), Expect = 3e-95
 Identities = 182/182 (100%)
 Strand = Plus / Plus


Query: 552 tttaatataaattataataatttaccagcagaatataggaagggttctgttatctttaag 611
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tttaatataaattataataatttaccagcagaatataggaagggttctgttatctttaag 60


Query: 612 aaaccagtacaagaaactaataaagaaactggcttaacaaaatcaaaaaagggattagtt 671
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aaaccagtacaagaaactaataaagaaactggcttaacaaaatcaaaaaagggattagtt 120


Query: 672 attgaacatgttgacattatttctgaaaaattttggaaagaataccctgatattttagct 731
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 attgaacatgttgacattatttctgaaaaattttggaaagaataccctgatattttagct 180


Query: 732 tc 733
           ||
Sbjct: 181 tc 182

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 913,818,051
Number of extensions: 57625209
Number of successful extensions: 4843548
Number of sequences better than 10.0: 135
Length of query: 822
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 798
Effective length of database: 97,308,875,965
Effective search space: 77652483020070
Effective search space used: 77652483020070
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.25
Homology vs Protein 
Query= Contig-U09386-1 (Contig-U09386-1Q) /CSM_Contig/Contig-U09386-1Q.Seq.d
         (822 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54E29) RecName: Full=Probable tRNA(His) guanylyltransferase;  ...   402   e-111
AJ720720_1(AJ720720|pid:none) Gallus gallus mRNA for hypothetica...   223   8e-57
BC085384_1(BC085384|pid:none) Danio rerio zgc:101609, mRNA (cDNA...   219   1e-55
AK000553_1(AK000553|pid:none) Homo sapiens cDNA FLJ20546 fis, cl...   212   1e-53
BT079599_1(BT079599|pid:none) Esox lucius clone eluc-evq-524-292...   211   3e-53
BT077177_1(BT077177|pid:none) Caligus rogercresseyi clone crog-e...   210   5e-53
AK223119_1(AK223119|pid:none) Homo sapiens mRNA for interphase c...   210   5e-53
BC001523_1(BC001523|pid:none) Homo sapiens tRNA-histidine guanyl...   208   2e-52
(Q9V3N8) RecName: Full=Probable tRNA(His) guanylyltransferase;  ...   207   3e-52
(Q9CY52) RecName: Full=Probable tRNA(His) guanylyltransferase;  ...   207   4e-52
(Q5M965) RecName: Full=Probable tRNA(His) guanylyltransferase;  ...   207   4e-52
BT082287_1(BT082287|pid:none) Anoplopoma fimbria clone afim-evh-...   206   1e-51
BT046440_1(BT046440|pid:none) Salmo salar clone ssal-evf-542-345...   205   2e-51
BT074325_1(BT074325|pid:none) Oncorhynchus mykiss clone omyk-evo...   204   4e-51
AM269985_4(AM269985|pid:none) Aspergillus niger contig An01c0380...   202   1e-50
DQ311278_1(DQ311278|pid:none) Bombyx mori interphase cyctoplasmi...   201   2e-50
BC085067_1(BC085067|pid:none) Xenopus laevis hypothetical LOC495...   198   2e-49
BT077855_1(BT077855|pid:none) Lepeophtheirus salmonis clone lsal...   196   8e-49
BT077537_1(BT077537|pid:none) Lepeophtheirus salmonis clone lsal...   194   2e-48
AE017343_282(AE017343|pid:none) Cryptococcus neoformans var. neo...   188   2e-46
AC097112_4(AC097112|pid:none) Oryza sativa (japonica cultivar-gr...   181   2e-44
AL844506_225(AL844506|pid:none) Plasmodium falciparum 3D7 chromo...   181   3e-44
AC007071_10(AC007071|pid:none) Arabidopsis thaliana chromosome 2...   170   4e-41
AP006852_54(AP006852|pid:none) Candida albicans genomic DNA, chr...   165   1e-39
(P53215) RecName: Full=tRNA(His) guanylyltransferase;          E...   163   5e-39
FM992694_10(FM992694|pid:none) Candida dubliniensis CD36 chromos...   163   5e-39
AK015467_1(AK015467|pid:none) Mus musculus adult male testis cDN...   162   1e-38
E84722(E84722)hypothetical protein At2g31580 [imported] - Arabid...   159   1e-37
(Q75DJ3) RecName: Full=tRNA(His) guanylyltransferase;          E...   156   9e-37
CU928171_726(CU928171|pid:none) Kluyveromyces thermotolerans str...   154   3e-36
CU928173_509(CU928173|pid:none) Zygosaccharomyces rouxii strain ...   154   3e-36
CR382131_401(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   153   7e-36
(Q6CW75) RecName: Full=tRNA(His) guanylyltransferase;          E...   152   2e-35
AK007233_1(AK007233|pid:none) Mus musculus adult male testis cDN...   136   7e-31
AK227925_1(AK227925|pid:none) Arabidopsis thaliana mRNA for hypo...   119   9e-26
BT014750_1(BT014750|pid:none) Arabidopsis thaliana At2g32320 mRN...   106   1e-21
FN357545_17(FN357545|pid:none) Schistosoma mansoni genome sequen...   102   1e-20
AK023355_1(AK023355|pid:none) Homo sapiens cDNA FLJ13293 fis, cl...   100   1e-19
AC097111_12(AC097111|pid:none) Oryza sativa (japonica cultivar-g...    94   7e-18
AK304590_1(AK304590|pid:none) Homo sapiens cDNA FLJ57781 complet...    79   2e-13
CP000113_5817(CP000113|pid:none) Myxococcus xanthus DK 1622, com...    60   7e-08
CP000102_1108(CP000102|pid:none) Methanosphaera stadtmanae DSM 3...    55   4e-06
AE009439_93(AE009439|pid:none) Methanopyrus kandleri AV19, compl...    52   2e-05
CP000254_585(CP000254|pid:none) Methanospirillum hungatei JF-1, ...    49   2e-04
CP000968_1190(CP000968|pid:none) Candidatus Korarchaeum cryptofi...    47   7e-04
CR937009_8(CR937009|pid:none) uncultured archaeon fos0644c1.           45   0.002
DQ443198_1(DQ443198|pid:none) Bombyx mori interphase cyctoplasmi...    45   0.003
EU197055_314(EU197055|pid:none) Pseudomonas phage 201phi2-1, com...    45   0.003
CP000559_752(CP000559|pid:none) Methanocorpusculum labreanum Z, ...    42   0.018
CP000780_2260(CP000780|pid:none) Candidatus Methanoregula boonei...    41   0.054
AP006618_3961(AP006618|pid:none) Nocardia farcinica IFM 10152 DN...    40   0.092
CP000395_113(CP000395|pid:none) Borrelia afzelii PKo, complete g...    35   2.9
(O51141) RecName: Full=Single-stranded DNA-binding protein;     ...    35   3.9
CP000828_3665(CP000828|pid:none) Acaryochloris marina MBIC11017,...    34   6.6

>(Q54E29) RecName: Full=Probable tRNA(His) guanylyltransferase;
               EC=2.7.7.-; AltName: Full=tRNA-histidine
           guanylyltransferase;
          Length = 256

 Score =  402 bits (1033), Expect = e-111
 Identities = 204/256 (79%), Positives = 204/256 (79%), Gaps = 37/256 (14%)
 Frame = +3

Query: 81  MANSKYEYVKSFEQPDILLQNVWIVVRIDGRSFHKFTTKHDYAKPNDDRGLSLMNRAALE 260
           MANSKYEYVKSFEQPDILLQNVWIVVRIDGRSFHKFTTKHDYAKPNDDRGLSLMNRAALE
Sbjct: 1   MANSKYEYVKSFEQPDILLQNVWIVVRIDGRSFHKFTTKHDYAKPNDDRGLSLMNRAALE 60

Query: 261 VCKEFPDIVIAFGESDEYSFVLKKSCNLFERRXXXXXXXXXXYFTSQFVYRWKEYFGEHE 440
           VCKEFPDIVIAFGESDEYSFVLKKSCNLFERR          YFTSQFVYRWKEYFGEHE
Sbjct: 61  VCKEFPDIVIAFGESDEYSFVLKKSCNLFERRSSKISSSIVSYFTSQFVYRWKEYFGEHE 120

Query: 441 LKYPPTFDSRCVLYPTDENIKDYLSWRQADTHI--------------------------- 539
           LKYPPTFDSRCVLYPTDENIKDYLSWRQADTHI
Sbjct: 121 LKYPPTFDSRCVLYPTDENIKDYLSWRQADTHINNLYNTCYWALVLKAGKTPIEAENELR 180

Query: 540 ----------XXXXFNINYNNLPAEYRKGSVIFKKPVQETNKETGLTKSKKGLVIEHVDI 689
                         FNINYNNLPAEYRKGSVIFKKPVQETNKETGLTKSKK LVIEHVDI
Sbjct: 181 GTFSDGKNEMLFSRFNINYNNLPAEYRKGSVIFKKPVQETNKETGLTKSKKRLVIEHVDI 240

Query: 690 ISEKFWKEYPDILASK 737
           ISEKFWKEYPDILASK
Sbjct: 241 ISEKFWKEYPDILASK 256

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,108,415,538
Number of extensions: 20614148
Number of successful extensions: 77667
Number of sequences better than 10.0: 54
Number of HSP's gapped: 77531
Number of HSP's successfully gapped: 94
Length of query: 274
Length of database: 1,040,966,779
Length adjustment: 127
Effective length of query: 147
Effective length of database: 634,022,584
Effective search space: 93201319848
Effective search space used: 93201319848
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.64  gvh: 0.39  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for Contig-U09386-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0