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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U09220-1
Contig ID Contig-U09220-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U09220-1 (Contig-U09220-1Q) /CSM_Contig/Contig-U09220-1Q.Seq.d
AATCCATTAAAATGGTAATTACCTCTAGCACACCACCATCCTCAACAACA
GAACAATTAATTTCTTCAATAAAGCCTTCAAATACACCTTGTTCACAATC
CCCTAAAAAATCTGTCGAAAACGATAGTAGTGGTAGTAGTAGTAACAGCA
GTAATAATAATAATAATAATAATAACAATAATAATATTTATAANAANAAT
NTTTTCAATAATAATAATAATAATAGTGACAGCAGTAGTAATAATAATAC
TTGCAATATAAGTAGAGAGAATTCGTTTCCTTTCATGATGGATTTAACAT
TAGAAGAAGCAGTTGCTGTAGAAGAGAATAATGAAACCTATAATAATAGT
AATAATAATAATAGTTGTGACAATAATAATAACAACAGCAGAAATGAAGA
GATATTAAATTCTAAATCCGAAATTTTAATAAATGAAAGCTATAATTGTA
CTAATAACAATAACAATAATAATAA----------ATAATCAAAATAATC
CAATTACTAATAGTATGATTGAAGATTCAATTGATATTGATAGATTAAAT
AAAAATGATAGTACAATGACTTCTTCAGTTATTAATCAACATTATCAACA
TCATGATTCACATGTTGAAGGCCAACATGATCAACAACAAGCACAACAAC
AGTCAACTCAAATACGACAACAACAGGCACAACAACAATTAGATATGGAA
CATCTTAGAAATAATGATATTCTAGTTATTAAACATTTAAATATTAGATA
CATTTGTGATTTAATGGTTGAAAATAAAAAGAAAAAGTAAGTAATAAATA
ATAATAATAATTATAAT
Gap gap included
Contig length 807
Chromosome number (1..6, M) 5
Chromosome length 5062330
Start point 3237404
End point 3236596
Strand (PLUS/MINUS) MINUS
Number of clones 2
Number of EST 3
Link to clone list U09220
List of clone(s) 

est1=SLH313F,1,191
est2=SLH316F,1,476
est3=SLH316Z,477,809
Translated Amino Acid sequence 
SIKMVITSSTPPSSTTEQLISSIKPSNTPCSQSPKKSVENDSSGSSSNSSNNNNNNNNNN
NIYXXNXFNNNNNNSDSSSNNNTCNISRENSFPFMMDLTLEEAVAVEENNETYNNSNNNN
SCDNNNNNSRNEEILNSKSEILINESYNCTNNNNNNN---

---NQNNPITNSMIEDSIDIDRLNKNDSTMTSSVINQHYQHHDSHVEGQHDQQQAQQQST
QIRQQQAQQQLDMEHLRNNDILVIKHLNIRYICDLMVENKKKK*vinnnnnyn
Translated Amino Acid sequence (All Frames) 
Frame A:
nplkw*lplahhhpqqqnn*flq*slqihlvhnplknlsktivvvvvvtaviiiiiiiti
iifixxxfsiiiiiivtavviiilai*verirfls*wi*h*kkqll*krimkpiiiviii
ivvtiiittaemkry*ilnpkf**mkaiivlititiii---

---iikiiqlliv*lkiqlilid*ikmivq*llqlliniinimihmlkanminnkhnnsq
lkydnnrhnnn*iwnileimif*llni*ildtfvi*wlkikrksk**iiiiii

Frame B:
ih*ngnyl*httilnnrtinffnkafkytlftip*kicrkr**w****qq********q*
*yl*xxxfq*******qq****ylqyk*refvsfhdgfnirrssccrre**nl*******
*l*q***qqqk*rdikf*irnfnk*kl*ly**q*q***---

---*sk*sny**yd*rfn*y**ik*k**yndffsy*stlsts*ftc*rpt*sttstttvn
snttttgtttirygts*k**yssy*tfky*ihl*fng*k*kekvsnk****l*

Frame C:
SIKMVITSSTPPSSTTEQLISSIKPSNTPCSQSPKKSVENDSSGSSSNSSNNNNNNNNNN
NIYXXNXFNNNNNNSDSSSNNNTCNISRENSFPFMMDLTLEEAVAVEENNETYNNSNNNN
SCDNNNNNSRNEEILNSKSEILINESYNCTNNNNNNN---

---NQNNPITNSMIEDSIDIDRLNKNDSTMTSSVINQHYQHHDSHVEGQHDQQQAQQQST
QIRQQQAQQQLDMEHLRNNDILVIKHLNIRYICDLMVENKKKK*vinnnnnyn
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U09220-1 (Contig-U09220-1Q)
/CSM_Contig/Contig-U09220-1Q.Seq.d
         (817 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U09220-1 (Contig-U09220-1Q) /CSM_Contig/Conti...   299  2e-81
Contig-U06294-1 (Contig-U06294-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U05819-1 (Contig-U05819-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U14519-1 (Contig-U14519-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U11270-1 (Contig-U11270-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U11258-1 (Contig-U11258-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U10898-1 (Contig-U10898-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U07909-1 (Contig-U07909-1Q) /CSM_Contig/Conti...    40  0.004
Contig-U12838-1 (Contig-U12838-1Q) /CSM_Contig/Conti...    38  0.015
Contig-U11408-1 (Contig-U11408-1Q) /CSM_Contig/Conti...    38  0.015

>Contig-U09220-1 (Contig-U09220-1Q) /CSM_Contig/Contig-U09220-1Q.Seq.d
          Length = 817

 Score =  299 bits (151), Expect = 2e-81
 Identities = 151/151 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   aatccattaaaatggtaattacctctagcacaccaccatcctcaacaacagaacaattaa 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aatccattaaaatggtaattacctctagcacaccaccatcctcaacaacagaacaattaa 60

                                                                       
Query: 61  tttcttcaataaagccttcaaatacaccttgttcacaatcccctaaaaaatctgtcgaaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tttcttcaataaagccttcaaatacaccttgttcacaatcccctaaaaaatctgtcgaaa 120

                                          
Query: 121 acgatagtagtggtagtagtagtaacagcag 151
           |||||||||||||||||||||||||||||||
Sbjct: 121 acgatagtagtggtagtagtagtaacagcag 151


 Score =  258 bits (130), Expect = 8e-69
 Identities = 130/130 (100%)
 Strand = Plus / Plus

                                                                       
Query: 513 gtatgattgaagattcaattgatattgatagattaaataaaaatgatagtacaatgactt 572
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 513 gtatgattgaagattcaattgatattgatagattaaataaaaatgatagtacaatgactt 572

                                                                       
Query: 573 cttcagttattaatcaacattatcaacatcatgattcacatgttgaaggccaacatgatc 632
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 573 cttcagttattaatcaacattatcaacatcatgattcacatgttgaaggccaacatgatc 632

                     
Query: 633 aacaacaagc 642
           ||||||||||
Sbjct: 633 aacaacaagc 642


 Score =  161 bits (81), Expect = 1e-39
 Identities = 81/81 (100%)
 Strand = Plus / Plus

                                                                       
Query: 689 ttagatatggaacatcttagaaataatgatattctagttattaaacatttaaatattaga 748
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 689 ttagatatggaacatcttagaaataatgatattctagttattaaacatttaaatattaga 748

                                
Query: 749 tacatttgtgatttaatggtt 769
           |||||||||||||||||||||
Sbjct: 749 tacatttgtgatttaatggtt 769


 Score =  151 bits (76), Expect = 1e-36
 Identities = 76/76 (100%)
 Strand = Plus / Plus

                                                                       
Query: 250 cttgcaatataagtagagagaattcgtttcctttcatgatggatttaacattagaagaag 309
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 250 cttgcaatataagtagagagaattcgtttcctttcatgatggatttaacattagaagaag 309

                           
Query: 310 cagttgctgtagaaga 325
           ||||||||||||||||
Sbjct: 310 cagttgctgtagaaga 325


 Score = 73.8 bits (37), Expect = 3e-13
 Identities = 37/37 (100%)
 Strand = Plus / Plus

                                                
Query: 383 caacagcagaaatgaagagatattaaattctaaatcc 419
           |||||||||||||||||||||||||||||||||||||
Sbjct: 383 caacagcagaaatgaagagatattaaattctaaatcc 419


>Contig-U06294-1 (Contig-U06294-1Q) /CSM_Contig/Contig-U06294-1Q.Seq.d
          Length = 613

 Score = 42.1 bits (21), Expect = 0.001
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 124 atagtagtggtagtagtagta 144
           |||||||||||||||||||||
Sbjct: 352 atagtagtggtagtagtagta 372


>Contig-U05819-1 (Contig-U05819-1Q) /CSM_Contig/Contig-U05819-1Q.Seq.d
          Length = 790

 Score = 42.1 bits (21), Expect = 0.001
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                    
Query: 518 attgaagattcaattgatattgata 542
           ||||||||||||||| |||||||||
Sbjct: 265 attgaagattcaattaatattgata 289


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8584
Number of Sequences: 6905
Number of extensions: 8584
Number of successful extensions: 1226
Number of sequences better than 10.0: 188
length of query: 817
length of database: 5,674,871
effective HSP length: 16
effective length of query: 801
effective length of database: 5,564,391
effective search space: 4457077191
effective search space used: 4457077191
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.15
Homology vs DNA 
Query= Contig-U09220-1 (Contig-U09220-1Q) /CSM_Contig/Contig-U09220-1Q.Seq.d
         (817 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU062041) Dictyostelium discoideum slug cDNA, clone SLH316.      295   e-117  3
(AU039268) Dictyostelium discoideum slug cDNA, clone SLH316.      272   e-109  2
(AU062040) Dictyostelium discoideum slug cDNA, clone SLH313.      299   9e-77  1
(AC180825) Strongylocentrotus purpuratus clone R3-3071D01, W...    54   0.008  1
(AC180398) Strongylocentrotus purpuratus clone R3-3107G20, W...    54   0.008  1
(AC179105) Strongylocentrotus purpuratus clone R3-4011E19, W...    54   0.008  1
(AC079319) Homo sapiens 12q BAC RP11-118A3 (Roswell Park Can...    52   0.033  1
(AF182340) Xenopus laevis clathrin assembly protein AP180 (A...    40   0.086  2
(AF079445) Dictyostelium discoideum TipC (tipC) gene, comple...    46   0.088  4
(AP007920) Lotus japonicus genomic DNA, chromosome 5, clone:...    40   0.099  3
(EK195209) 1095460035987 Global-Ocean-Sampling_GS-31-01-01-1...    50   0.13   1
(EH617303) CHO_SL006xc06f1.ab1 CHO_SL Nicotiana tabacum cDNA...    50   0.13   1
(EE007427) ROE00002308 Rhizopus oryzae Company Rhizopus oryz...    50   0.13   1
(EE002130) ROE00011291 Rhizopus oryzae Company Rhizopus oryz...    50   0.13   1
(EC999572) ROE00011195 Rhizopus oryzae Company Rhizopus oryz...    50   0.13   1
(AE013599) Drosophila melanogaster chromosome 2R, complete s...    36   0.22   2
(AC007418) Drosophila melanogaster clone BACR16P22, complete...    36   0.30   2
(AC009208) Drosophila melanogaster clone BACR31I21, complete...    36   0.30   2
(AC015232) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    36   0.33   2
(CZ481728) e03969-5prime Exelixis piggyBac RB insertions Dro...    36   0.39   2
(AC226683) Pongo abelii chromosome UNKNOWN clone CH276-456D2...    48   0.39   3
(CR391951) Zebrafish DNA sequence from clone DKEY-16N5 in li...    48   0.52   1
(AL974314) Zebrafish DNA sequence from clone DKEY-89P3 in li...    48   0.52   1
(BS000055) Pan troglodytes chromosome 22 clone:RP43-136G21, ...    48   0.52   1
(AP001677) Homo sapiens genomic DNA, chromosome 21q, section...    48   0.52   1
(AP000568) Homo sapiens genomic DNA, chromosome 21q21.1-q21....    48   0.52   1
(AC068736) Homo sapiens clone RP11-228P15, LOW-PASS SEQUENCE...    48   0.52   1
(AC068132) Homo sapiens chromosome 21 clone RP11-753B2, WORK...    48   0.52   1
(AC211385) Pongo abelii chromosome UNKNOWN clone CH276-119C5...    48   0.52   1
(AC201562) Strongylocentrotus purpuratus clone R3-3122O7, WO...    48   0.52   1
(AC181793) Strongylocentrotus purpuratus clone R3-1029G23, W...    48   0.52   1
(AC180512) Strongylocentrotus purpuratus clone R3-3016D16, W...    48   0.52   1
(AC180284) Strongylocentrotus purpuratus clone R3-3121A18, W...    48   0.52   1
(AC179835) Strongylocentrotus purpuratus clone R3-1012D21, W...    48   0.52   1
(AC179204) Strongylocentrotus purpuratus clone R3-4019M1, WO...    48   0.52   1
(AC178730) Strongylocentrotus purpuratus clone R3-13D8, WORK...    48   0.52   1
(AC178117) Strongylocentrotus purpuratus clone R3-3074G14, W...    48   0.52   1
(AZ192856) SP_1022_A2_F07_T7A Strongylocentrotus purpuratus,...    48   0.52   1
(EK222881) 1095460146662 Global-Ocean-Sampling_GS-31-01-01-1...    48   0.52   1
(CE732596) tigr-gss-dog-17000329845185 Dog Library Canis lup...    48   0.52   1
(AG018494) Homo sapiens genomic DNA, 21q region, clone: B335...    48   0.52   1
(AG018492) Homo sapiens genomic DNA, 21q region, clone: B335...    48   0.52   1
(AG018470) Homo sapiens genomic DNA, 21q region, clone: B335...    48   0.52   1
(AG018469) Homo sapiens genomic DNA, 21q region, clone: B335...    48   0.52   1
(AC025907) Oryza sativa Japonica Group chromosome 10 clone n...    44   0.65   4
(AC176717) Strongylocentrotus purpuratus clone R3-3095K14, W...    40   1.3    4
(AK049289) Mus musculus ES cells cDNA, RIKEN full-length enr...    38   1.7    2
(CR376827) Zebrafish DNA sequence from clone CH211-154C21 in...    40   1.8    4
(AC000112) Human PAC clone DJ149P21, complete sequence.            40   1.8    4
(AC181501) Strongylocentrotus purpuratus clone R3-1111D3, WO...    40   2.0    3
(AC115683) Dictyostelium discoideum chromosome 2 map complem...    38   2.0    3
(CT826380) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    36   2.0    5
(BX255950) Zebrafish DNA sequence from clone CH211-250K6 in ...    46   2.1    1
(AL935049) Zebrafish DNA sequence from clone CH211-185K2 in ...    46   2.1    1
(AL928716) Zebrafish DNA sequence from clone DKEY-23K6 in li...    46   2.1    1
(AL683845) Mouse DNA sequence from clone RP23-423B1 on chrom...    46   2.1    1
(AJ421479) Mus musculus X-inactivation center region; segmen...    46   2.1    1
(AC132351) Mus musculus BAC clone RP24-95F24 from chromosome...    46   2.1    1
(AC130025) Rattus norvegicus  BAC CH230-44M13 (Children's Ho...    46   2.1    1
(AC126450) Mus musculus BAC clone RP23-379J7 from 3, complet...    46   2.1    1
(AC121971) Mus musculus BAC clone RP24-275H22 from chromosom...    46   2.1    1
(AC105968) Mus musculus chromosome 18, clone RP23-265P4, com...    46   2.1    1
(EU888845) Artemia parthenogenetica clone Apdq02TAIL microsa...    46   2.1    1
(AM910995) Plasmodium knowlesi strain H chromosome 13, compl...    46   2.1    1
(AC157958) Strongylocentrotus purpuratus clone R3-4002H9, WO...    46   2.1    1
(AC132176) Rattus norvegicus clone CH230-142C4, WORKING DRAF...    46   2.1    1
(AC131146) Rattus norvegicus clone CH230-62A9, WORKING DRAFT...    46   2.1    1
(AC127010) Rattus norvegicus clone CH230-478D15, *** SEQUENC...    46   2.1    1
(AC118791) Rattus norvegicus clone CH230-179K2, *** SEQUENCI...    46   2.1    1
(AC114504) Rattus norvegicus clone CH230-236J11, WORKING DRA...    46   2.1    1
(AC111776) Rattus norvegicus clone CH230-153O13, *** SEQUENC...    46   2.1    1
(AC107265) Rattus norvegicus clone CH230-26A18, *** SEQUENCI...    46   2.1    1
(AC098758) Rattus norvegicus clone CH230-131E1, WORKING DRAF...    46   2.1    1
(AC095944) Rattus norvegicus clone CH230-10P24, WORKING DRAF...    46   2.1    1
(AC094584) Rattus norvegicus clone CH230-4B8, *** SEQUENCING...    46   2.1    1
(CU915265) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    46   2.1    1
(CU012037) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   2.1    1
(AC184601) Strongylocentrotus purpuratus clone R3-3100G10, W...    46   2.1    1
(AC184370) Strongylocentrotus purpuratus clone R3-1011K1, WO...    46   2.1    1
(AC181859) Strongylocentrotus purpuratus clone R3-1020A24, W...    46   2.1    1
(AC181478) Strongylocentrotus purpuratus clone R3-51K1, WORK...    46   2.1    1
(AC179577) Strongylocentrotus purpuratus clone R3-8P21, WORK...    46   2.1    1
(AC178650) Strongylocentrotus purpuratus clone R3-3007L22, W...    46   2.1    1
(AC177656) Strongylocentrotus purpuratus clone R3-1020B11, W...    46   2.1    1
(AC177222) Strongylocentrotus purpuratus clone R3-40I7, WORK...    46   2.1    1
(AC177047) Strongylocentrotus purpuratus clone R3-38P16, WOR...    46   2.1    1
(AC176918) Strongylocentrotus purpuratus clone R3-33D5, WORK...    46   2.1    1
(AC176665) Strongylocentrotus purpuratus clone R3-3084J24, W...    46   2.1    1
(AC176331) Strongylocentrotus purpuratus clone R3-3053I3, WO...    46   2.1    1
(AC176137) Strongylocentrotus purpuratus clone R3-28G13, WOR...    46   2.1    1
(AC174554) Strongylocentrotus purpuratus clone R3-1008C22, W...    46   2.1    1
(AC173849) Strongylocentrotus purpuratus clone R3-3022J22, W...    46   2.1    1
(AC168790) Strongylocentrotus purpuratus clone R3-13K10, WOR...    46   2.1    1
(BH371981) AG-ND-165P4.TF ND-TAM Anopheles gambiae genomic c...    46   2.1    1
(DX485313) Cpl27034 Papaya Genomic DNA BAC Library Carica pa...    46   2.1    1
(DH708796) Rattus norvegicus DNA, BAC clone: RNB1-271M01, 3'...    46   2.1    1
(CT219160) Sus scrofa genomic clone PigE-235A11, genomic sur...    46   2.1    1
(CT121733) Sus scrofa genomic clone PigE-235A11, genomic sur...    46   2.1    1
(CL697824) SP__Ba0017L16.f SP__Ba Sorghum propinquum genomic...    46   2.1    1
(AM955468) Sparus aurata EST, clone cDN03P0002L24, 5'-end se...    46   2.1    1

>(AU062041) Dictyostelium discoideum slug cDNA, clone SLH316.
          Length = 476

 Score =  295 bits (149), Expect(3) = e-117
 Identities = 149/149 (100%)
 Strand = Plus / Plus


Query: 3   tccattaaaatggtaattacctctagcacaccaccatcctcaacaacagaacaattaatt 62
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3   tccattaaaatggtaattacctctagcacaccaccatcctcaacaacagaacaattaatt 62


Query: 63  tcttcaataaagccttcaaatacaccttgttcacaatcccctaaaaaatctgtcgaaaac 122
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 63  tcttcaataaagccttcaaatacaccttgttcacaatcccctaaaaaatctgtcgaaaac 122


Query: 123 gatagtagtggtagtagtagtaacagcag 151
           |||||||||||||||||||||||||||||
Sbjct: 123 gatagtagtggtagtagtagtaacagcag 151

 Score =  129 bits (65), Expect(3) = e-117
 Identities = 65/65 (100%)
 Strand = Plus / Plus


Query: 262 gtagagagaattcgtttcctttcatgatggatttaacattagaagaagcagttgctgtag 321
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 262 gtagagagaattcgtttcctttcatgatggatttaacattagaagaagcagttgctgtag 321


Query: 322 aagag 326
           |||||
Sbjct: 322 aagag 326

 Score = 60.0 bits (30), Expect(3) = e-117
 Identities = 30/30 (100%)
 Strand = Plus / Plus


Query: 383 caacagcagaaatgaagagatattaaattc 412
           ||||||||||||||||||||||||||||||
Sbjct: 383 caacagcagaaatgaagagatattaaattc 412

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 570,193,309
Number of extensions: 35993185
Number of successful extensions: 3273176
Number of sequences better than 10.0: 223
Length of query: 817
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 793
Effective length of database: 97,308,875,965
Effective search space: 77165938640245
Effective search space used: 77165938640245
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.25
Homology vs Protein 
Query= Contig-U09220-1 (Contig-U09220-1Q) /CSM_Contig/Contig-U09220-1Q.Seq.d
         (817 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC108606_1(BC108606|pid:none) Xenopus laevis hypothetical protei...    35   2.2
AY811631_1(AY811631|pid:none) Schistosoma japonicum SJCHGC05866 ...    33   8.5
FN357549_33(FN357549|pid:none) Schistosoma mansoni genome sequen...    33   8.5

>BC108606_1(BC108606|pid:none) Xenopus laevis hypothetical protein
           MGC131146, mRNA (cDNA clone MGC:131146 IMAGE:7207059),
           complete cds.
          Length = 574

 Score = 35.4 bits (80), Expect = 2.2
 Identities = 13/25 (52%), Positives = 22/25 (88%)
 Frame = +2

Query: 698 EHLRNNDILVIKHLNIRYICDLMVE 772
           + +R +DI ++++L++RYICDLMVE
Sbjct: 169 DKVRGHDIKLLRYLSVRYICDLMVE 193

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 478,702,060
Number of extensions: 4283575
Number of successful extensions: 11253
Number of sequences better than 10.0: 3
Number of HSP's gapped: 11224
Number of HSP's successfully gapped: 3
Length of query: 272
Length of database: 1,040,966,779
Length adjustment: 126
Effective length of query: 146
Effective length of database: 637,226,869
Effective search space: 93035122874
Effective search space used: 93035122874
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.88  gvh: 0.46  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.39  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for Contig-U09220-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 2
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0