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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U09091-1
Contig ID Contig-U09091-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U09091-1 (Contig-U09091-1Q) /CSM_Contig/Contig-U09091-1Q.Seq.d
ATTAATAAATTTATTACAACGAATATTTTTGTGAGACTTAATTTGGTATT
AATTTGTAAATATATAAAAAAAACCCCTCTCTCCCCCTCTCTCAAAAAAA
AAATATAAAAAAAAATTATAATAAATCCCCACAAANGTAGTAATAATAAA
AAAAAAANCAANTAANGAAAAANGATAANGAAANTTTANTNTNTNTNTNT
AANGAANGAAANGTCNTTAAAAA----------TCCCNATNTANACTACC
NCTGGNCAAANTAAAACTGGTTTNGCNCCNANTGCAANCGGNTTCCCCTT
ANGNTTCCNTTGNTTCTTGGGNGGNTCANNCTCTGGNGGNGCNTTCAANC
CNGCTAAANNTTTCGGGACNGCNTTANTTGGNAANNATTGGACNCGNCNT
TGGANGNATTGGAAANCNGNCCTTTTAGGGGCNGGNTTANCNGGNTTTGC
CCNAAAANTCNTTTCNNCNACNCANAAATAAATTTCNAAAAAANAAAAAA
AAANAATTCCAANAAAAAAAATAAATAATAATAATAATAATAAAAACTAA
ATA
Gap gap included
Contig length 543
Chromosome number (1..6, M) -
Chromosome length -
Start point -
End point -
Strand (PLUS/MINUS) -
Number of clones 1
Number of EST 2
Link to clone list U09091
List of clone(s) 

est1=SFJ471F,1,224
est2=SFJ471Z,225,545
Translated Amino Acid sequence 
**iyyneyfcet*fginl*iykknpslplsqkknikknynkspqx****KKXQXXKXDXE
XLXXXXXEXXVXK---

---PXXTTXGQXKTGXAPXAXGFPLXFXXFLGGSXSGGAFXPAKXFGTALXGXXWTRXWX
XWKXXLLGAGLXGFAXKXXSXTXK*iskkxkkxipxkkinnnnnnkn*i
Translated Amino Acid sequence (All Frames) 
Frame A:
inkfittnifvrlnlvlickyikktplspslkkki*kkiiinphkxsnnkkkxx*xkxix
kxxxxxxxxexslk---

---sxxxlplxkxklvxxxxqxxspxxsxxswxxxxlxxxsxxlxxsgxxxlxxigxxxg
xigxxxf*gxxxxxlpxxsfxxxxnkfxkxkkkxfqxkk*iiiiiiktk

Frame B:
linllqrifl*dliwy*fvni*kkplspplskkkykkkl**iptxvviikkkxxxekx*x
xfxxxx*xxkxx*k---

---pxxxyxwxx*nwfxxxcxrxplxxplxlgxxxlwxxxqxx*xfrdxxxwxxldxxlx
xlexxpfrgxxxxxcpkxxfxxxxinfxkxkkxnsxkknk******kln

Frame C:
**iyyneyfcet*fginl*iykknpslplsqkknikknynkspqx****KKXQXXKXDXE
XLXXXXXEXXVXK---

---PXXTTXGQXKTGXAPXAXGFPLXFXXFLGGSXSGGAFXPAKXFGTALXGXXWTRXWX
XWKXXLLGAGLXGFAXKXXSXTXK*iskkxkkxipxkkinnnnnnkn*i
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U09091-1 (Contig-U09091-1Q)
/CSM_Contig/Contig-U09091-1Q.Seq.d
         (553 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U09091-1 (Contig-U09091-1Q) /CSM_Contig/Conti...   180  1e-45
Contig-U11766-1 (Contig-U11766-1Q) /CSM_Contig/Conti...    36  0.039
Contig-U10030-1 (Contig-U10030-1Q) /CSM_Contig/Conti...    32  0.60
Contig-U09402-1 (Contig-U09402-1Q) /CSM_Contig/Conti...    32  0.60
Contig-U04680-1 (Contig-U04680-1Q) /CSM_Contig/Conti...    32  0.60
Contig-U13764-1 (Contig-U13764-1Q) /CSM_Contig/Conti...    30  2.4
Contig-U13191-1 (Contig-U13191-1Q) /CSM_Contig/Conti...    30  2.4
Contig-U12633-1 (Contig-U12633-1Q) /CSM_Contig/Conti...    30  2.4
Contig-U12435-1 (Contig-U12435-1Q) /CSM_Contig/Conti...    30  2.4
Contig-U11341-1 (Contig-U11341-1Q) /CSM_Contig/Conti...    30  2.4

>Contig-U09091-1 (Contig-U09091-1Q) /CSM_Contig/Contig-U09091-1Q.Seq.d
          Length = 553

 Score =  180 bits (91), Expect = 1e-45
 Identities = 253/253 (100%)
 Strand = Plus / Plus

                                                                       
Query: 234 tcccnatntanactaccnctggncaaantaaaactggtttngcnccnantgcaancggnt 293
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 234 tcccnatntanactaccnctggncaaantaaaactggtttngcnccnantgcaancggnt 293

                                                                       
Query: 294 tccccttangnttccnttgnttcttgggnggntcannctctggnggngcnttcaanccng 353
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 294 tccccttangnttccnttgnttcttgggnggntcannctctggnggngcnttcaanccng 353

                                                                       
Query: 354 ctaaanntttcgggacngcnttanttggnaannattggacncgncnttggangnattgga 413
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 354 ctaaanntttcgggacngcnttanttggnaannattggacncgncnttggangnattgga 413

                                                                       
Query: 414 aancngnccttttaggggcnggnttancnggntttgcccnaaaantcntttcnncnacnc 473
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 414 aancngnccttttaggggcnggnttancnggntttgcccnaaaantcntttcnncnacnc 473

                        
Query: 474 anaaataaatttc 486
           |||||||||||||
Sbjct: 474 anaaataaatttc 486


 Score =  137 bits (69), Expect = 1e-32
 Identities = 85/93 (91%)
 Strand = Plus / Plus

                                                                      
Query: 1  attaataaatttattacaacgaatatttttgtgagacttaatttggtattaatttgtaaa 60
          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  attaataaatttattacaacgaatatttttgtgagacttaatttggtattaatttgtaaa 60

                                           
Query: 61 tatatnnnnnnnncccctctctccccctctctc 93
          |||||        ||||||||||||||||||||
Sbjct: 61 tatataaaaaaaacccctctctccccctctctc 93


 Score = 28.2 bits (14), Expect = 9.4
 Identities = 14/14 (100%)
 Strand = Plus / Minus

                        
Query: 3  taataaatttatta 16
          ||||||||||||||
Sbjct: 16 taataaatttatta 3


>Contig-U11766-1 (Contig-U11766-1Q) /CSM_Contig/Contig-U11766-1Q.Seq.d
          Length = 923

 Score = 36.2 bits (18), Expect = 0.039
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 47  tattaatttgtaaatata 64
           ||||||||||||||||||
Sbjct: 96  tattaatttgtaaatata 113


>Contig-U10030-1 (Contig-U10030-1Q) /CSM_Contig/Contig-U10030-1Q.Seq.d
          Length = 899

 Score = 32.2 bits (16), Expect = 0.60
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 50  taatttgtaaatatat 65
           ||||||||||||||||
Sbjct: 834 taatttgtaaatatat 819


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1723
Number of Sequences: 6905
Number of extensions: 1723
Number of successful extensions: 189
Number of sequences better than 10.0: 48
length of query: 553
length of database: 5,674,871
effective HSP length: 16
effective length of query: 537
effective length of database: 5,564,391
effective search space: 2988077967
effective search space used: 2988077967
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.11.14
Homology vs DNA 
Query= Contig-U09091-1 (Contig-U09091-1Q) /CSM_Contig/Contig-U09091-1Q.Seq.d
         (553 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ401876) Dictyostelium discoideum cDNA clone:dds19n18, 3' ...   100   6e-33  2
(BJ389685) Dictyostelium discoideum cDNA clone:dds19n18, 5' ...   129   1e-25  1
(AU061825) Dictyostelium discoideum slug cDNA, clone SLG243.       52   0.021  1
(AU039712) Dictyostelium discoideum slug cDNA, clone SLG243.       52   0.021  1
(AL772349) Mouse DNA sequence from clone RP23-466A17 on chro...    48   0.33   1
(AP000609) Homo sapiens genomic DNA, chromosome 11q, clone:C...    46   1.3    1
(ET406572) S0016K22_T7 CHORI-214 Salmo salar genomic clone S...    44   5.2    1
(ET275917) S0097M14_T7 CHORI-214 Salmo salar genomic clone S...    44   5.2    1
(EJ427561) 1093015235461 Global-Ocean-Sampling_GS-28-01-01-1...    44   5.2    1
(ED120744) AUAC-aai29h04.g1 Ascaris suum whole genome shotgu...    44   5.2    1
(CC256470) CH261-49E15_RM1.1 CH261 Gallus gallus genomic clo...    44   5.2    1
(CG818393) SOYAI10TV LargeInsertSoybeanGenLib Glycine max ge...    34   8.9    2

>(BJ401876) Dictyostelium discoideum cDNA clone:dds19n18, 3' end,
           single read.
          Length = 321

 Score = 99.6 bits (50), Expect(2) = 6e-33
 Identities = 143/143 (100%)
 Strand = Plus / Minus


Query: 344 ttcaanccngctaaanntttcgggacngcnttanttggnaannattggacncgncnttgg 403
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 211 ttcaanccngctaaanntttcgggacngcnttanttggnaannattggacncgncnttgg 152


Query: 404 angnattggaaancngnccttttaggggcnggnttancnggntttgcccnaaaantcntt 463
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 151 angnattggaaancngnccttttaggggcnggnttancnggntttgcccnaaaantcntt 92


Query: 464 tcnncnacncanaaataaatttc 486
           |||||||||||||||||||||||
Sbjct: 91  tcnncnacncanaaataaatttc 69

 Score = 73.8 bits (37), Expect(2) = 6e-33
 Identities = 85/85 (100%)
 Strand = Plus / Minus


Query: 234 tcccnatntanactaccnctggncaaantaaaactggtttngcnccnantgcaancggnt 293
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 tcccnatntanactaccnctggncaaantaaaactggtttngcnccnantgcaancggnt 262


Query: 294 tccccttangnttccnttgnttctt 318
           |||||||||||||||||||||||||
Sbjct: 261 tccccttangnttccnttgnttctt 237

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 131,621,864
Number of extensions: 7857273
Number of successful extensions: 539860
Number of sequences better than 10.0: 12
Length of query: 553
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 530
Effective length of database: 93,106,754,628
Effective search space: 49346579952840
Effective search space used: 49346579952840
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.25
Homology vs Protein 
Query= Contig-U09091-1 (Contig-U09091-1Q) /CSM_Contig/Contig-U09091-1Q.Seq.d
         (553 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB458833_1(AB458833|pid:none) Bombyx mori aqp mRNA for aquaporin...    34   2.2

>AB458833_1(AB458833|pid:none) Bombyx mori aqp mRNA for aquaporin
           AQP-Bom3, complete cds.
          Length = 270

 Score = 34.3 bits (77), Expect = 2.2
 Identities = 15/46 (32%), Positives = 19/46 (41%)
 Frame = +2

Query: 275 APXAXGFPLXFXXFLXXXXXXXAFXPAKXFGTALXGXXWTRXWXXW 412
           AP A G  +     L       A  PA+ FG+AL    W+  W  W
Sbjct: 174 APLAIGLTVTLGHLLAVDYTGSAMNPARSFGSALVASNWSHHWIYW 219

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 264,312,586
Number of extensions: 12941616
Number of successful extensions: 2900
Number of sequences better than 10.0: 1
Number of HSP's gapped: 2896
Number of HSP's successfully gapped: 1
Length of query: 184
Length of database: 1,040,966,779
Length adjustment: 121
Effective length of query: 63
Effective length of database: 653,248,294
Effective search space: 41154642522
Effective search space used: 41154642522
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.70  gvh: 0.65  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U09091-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 1
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0