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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U08712-1
Contig ID Contig-U08712-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U08712-1 (Contig-U08712-1Q) /CSM_Contig/Contig-U08712-1Q.Seq.d
TTAAGTGATAGTGATGATGAAGAAATTGTAATGAAGAAACCAAGAAGAGT
TTCAACACCAAGAAAGAAGAGAACTAGAGGTCAATTCTTTATGGATAGAG
ATAAAAGTGATGAGGAAAGAGGTGATGATACCTATCAAATTGATGATTTT
GTAGTTGAGAATGGTTCAGATGTTGATGGTTCAAGTGAAGAAGAGGAATT
AAAAAGAAAATCGAGAAGAAGAGATAGAAAAATAATAATAAATGATGAAA
AGGATAATGAGGAGGGAGAAGAAGAAGAAGAAGAGGAAGAAGTTTCAATT
CTTTATGAACCTAAAATTAAAGAAAGAGAAAAAATTAAAGAAGGACCAAT
TGATATTGATACAAATGAACCAATTTATTATACTCCACAATCTGGTTCAA
CTAAAAGATCATCAAGTATTTTAGATTATTTCAAAAGAGATGATAAAGAT
TATCATGATCATGATGATAATAAACCAACCACTACAACTACAACAACATC
ATCATCATTATTATCACCATCATCATTTGATGAGGATGATTTTTGAAAAT
AAGAAAAAAATGAAAGAATTTCAAAAACAACAACAAATGAAACAACAACC
AAACTCATCA----------CTCATCAAGATCCAAATTTTATTAAAGAAA
AAGTATTAAAAGAGTTTTTCTAAAAAGTTTACCATTAAAGAATCATTTTC
AATCTTTATTCAAATATGTAATTAGTTGTATTGTTGATGAAGAGTTTTCA
AGAGCAGTGATTGAAGATAAGGATGAATATTTCTTGGATGCAATTAATAG
AGTTAAAAGTTTTATCGTTAGTAAAAAGGAAGCATTGGTATTGGGTAATA
TGTGGCATGATGAATATTTACATGATATCACACATAGACCATTTCTTCAT
TCTGCCGAAGTTTCAGAATATGATTATGAAGGTAGAAAATGTAAATCATG
TAATAAAGCCAAACATAATGTTACCATCATTATCTATTTCTCTGGTAGAT
CCTATGATGTCGATGATTTTTGGAGTGGTTATTTTGAAAATCCAAAACGT
TTAAAAGGTACCACCAAAGATGAACCAAAATCAATCGAATATGAATTGGG
TTCATTCTGTTTCAAACGTTCACAACTTTTTCATCAACTCCATCATTTTC
CTTATAAATTCTTTAGAGATGTTAAACAAGATGTTAGAGATTTACAACAA
CTTCATTCGGATTGGACAAATCAAGATATTCTTTTTCATATGACCTCTCC
AGATAATAGTTATGTTTCAAGCTATTTTAACTTTTTAGAAAATTTA
Gap gap included
Contig length 1286
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 987321
End point 986106
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 2
Link to clone list U08712
List of clone(s) 

est1=AFD734F,1,611
est2=AFD734Z,612,1288
Translated Amino Acid sequence 
*vivmmkkl**rnqeefqhqerrelevnslwieikvmrkevmipiklmil*lrmvqmlmv
qvkkrn*kenreeeiek***mmkrimrrekkkkkrkkfqffmnlklkkekklkkdqlili
qmnqfiilhnlvqlkdhqvf*IISKEMIKIIMIMMIINQPLQLQQHHHHYYHHHHLMRMI
FENKKKMKEFQKQQQMKQQPNSS---

---HQDPNFIKEKVLKEFF*kvyh*riifnlysnm*lvvllmksfqeq*lkirmniswmq
lielkvlslvkrkhwywvicgmmniymishidhffilpkfqnmimkvenvnhvikpniml
pslsislvdpmmsmifgvvilkiqnv*kvppkmnqnqsnmnwvhsvsnvhnffinsiifl
inslemlnkmleiynnfirigqikifffi*plqiivmfqailtf*ki
Translated Amino Acid sequence (All Frames) 
Frame A:
lsdsddeeivmkkprrvstprkkrtrgqffmdrdksdeergddtyqiddfvvengsdvdg
sseeeelkrksrrrdrkiiindekdneegeeeeeeeevsilyepkikerekikegpidid
tnepiyytpqsgstkrsssildyfkrddkdyhdhddnkptttttttsssllspssfdedd
f*k*eknerisktttnetttkli---

---likiqillkkky*ksfskkftikesfsifiqicn*lyc**rvfkssd*r*g*iflgc
n**s*kfyr**kgsigig*yva**ift*yht*tissfcrsfri*l*r*km*im**sqt*c
yhhylflw*il*cr*flewlf*ksktfkryhqr*tkinri*igfilfqtfttfsstpsfs
l*il*rc*trc*rftttsfgldksrysfsydlsr**lcfklf*lfrkf

Frame B:
*vivmmkkl**rnqeefqhqerrelevnslwieikvmrkevmipiklmil*lrmvqmlmv
qvkkrn*kenreeeiek***mmkrimrrekkkkkrkkfqffmnlklkkekklkkdqlili
qmnqfiilhnlvqlkdhqvf*IISKEMIKIIMIMMIINQPLQLQQHHHHYYHHHHLMRMI
FENKKKMKEFQKQQQMKQQPNSS---

---ssrskfy*rksikrvflkslplknhfqslfkyviscivdeefsraviedkdeyflda
inrvksfivskkealvlgnmwhdeylhdithrpflhsaevseydyegrkckscnkakhnv
tiiiyfsgrsydvddfwsgyfenpkrlkgttkdepksieyelgsfcfkrsqlfhqlhhfp
ykffrdvkqdvrdlqqlhsdwtnqdilfhmtspdnsyvssyfnflenl

Frame C:
k*****rncneetkksfntkkeen*rsilyg*r*k**gkr**ylsn**fcs*ewfrc*wf
k*rrgikkkiekkr*knnnk**kg**ggrrrrrrgrsfnsl*t*n*rkrkn*rrtn*y*y
k*tnllystiwfn*kiikyfrlfqkr**rls*s****tnhynynniiiiiitiii**g*f
lkirkk*knfknnnk*nnnqth---

---HQDPNFIKEKVLKEFF*kvyh*riifnlysnm*lvvllmksfqeq*lkirmniswmq
lielkvlslvkrkhwywvicgmmniymishidhffilpkfqnmimkvenvnhvikpniml
pslsislvdpmmsmifgvvilkiqnv*kvppkmnqnqsnmnwvhsvsnvhnffinsiifl
inslemlnkmleiynnfirigqikifffi*plqiivmfqailtf*ki
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U08712-1 (Contig-U08712-1Q)
/CSM_Contig/Contig-U08712-1Q.Seq.d
         (1296 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08712-1 (Contig-U08712-1Q) /CSM_Contig/Conti...  1340  0.0
Contig-U01481-1 (Contig-U01481-1Q) /CSM_Contig/Conti...    48  2e-05
Contig-U01120-1 (Contig-U01120-1Q) /CSM_Contig/Conti...    48  2e-05
Contig-U14691-1 (Contig-U14691-1Q) /CSM_Contig/Conti...    46  1e-04
Contig-U13531-1 (Contig-U13531-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U12887-1 (Contig-U12887-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U11980-1 (Contig-U11980-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U11113-1 (Contig-U11113-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U09615-1 (Contig-U09615-1Q) /CSM_Contig/Conti...    44  4e-04
Contig-U06318-1 (Contig-U06318-1Q) /CSM_Contig/Conti...    44  4e-04

>Contig-U08712-1 (Contig-U08712-1Q) /CSM_Contig/Contig-U08712-1Q.Seq.d
          Length = 1296

 Score = 1340 bits (676), Expect = 0.0
 Identities = 676/676 (100%)
 Strand = Plus / Plus

                                                                        
Query: 621  ctcatcaagatccaaattttattaaagaaaaagtattaaaagagtttttctaaaaagttt 680
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 621  ctcatcaagatccaaattttattaaagaaaaagtattaaaagagtttttctaaaaagttt 680

                                                                        
Query: 681  accattaaagaatcattttcaatctttattcaaatatgtaattagttgtattgttgatga 740
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 681  accattaaagaatcattttcaatctttattcaaatatgtaattagttgtattgttgatga 740

                                                                        
Query: 741  agagttttcaagagcagtgattgaagataaggatgaatatttcttggatgcaattaatag 800
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 741  agagttttcaagagcagtgattgaagataaggatgaatatttcttggatgcaattaatag 800

                                                                        
Query: 801  agttaaaagttttatcgttagtaaaaaggaagcattggtattgggtaatatgtggcatga 860
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 801  agttaaaagttttatcgttagtaaaaaggaagcattggtattgggtaatatgtggcatga 860

                                                                        
Query: 861  tgaatatttacatgatatcacacatagaccatttcttcattctgccgaagtttcagaata 920
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 861  tgaatatttacatgatatcacacatagaccatttcttcattctgccgaagtttcagaata 920

                                                                        
Query: 921  tgattatgaaggtagaaaatgtaaatcatgtaataaagccaaacataatgttaccatcat 980
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 921  tgattatgaaggtagaaaatgtaaatcatgtaataaagccaaacataatgttaccatcat 980

                                                                        
Query: 981  tatctatttctctggtagatcctatgatgtcgatgatttttggagtggttattttgaaaa 1040
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 981  tatctatttctctggtagatcctatgatgtcgatgatttttggagtggttattttgaaaa 1040

                                                                        
Query: 1041 tccaaaacgtttaaaaggtaccaccaaagatgaaccaaaatcaatcgaatatgaattggg 1100
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1041 tccaaaacgtttaaaaggtaccaccaaagatgaaccaaaatcaatcgaatatgaattggg 1100

                                                                        
Query: 1101 ttcattctgtttcaaacgttcacaactttttcatcaactccatcattttccttataaatt 1160
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1101 ttcattctgtttcaaacgttcacaactttttcatcaactccatcattttccttataaatt 1160

                                                                        
Query: 1161 ctttagagatgttaaacaagatgttagagatttacaacaacttcattcggattggacaaa 1220
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1161 ctttagagatgttaaacaagatgttagagatttacaacaacttcattcggattggacaaa 1220

                                                                        
Query: 1221 tcaagatattctttttcatatgacctctccagataatagttatgtttcaagctattttaa 1280
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1221 tcaagatattctttttcatatgacctctccagataatagttatgtttcaagctattttaa 1280

                            
Query: 1281 ctttttagaaaattta 1296
            ||||||||||||||||
Sbjct: 1281 ctttttagaaaattta 1296


 Score =  500 bits (252), Expect = e-141
 Identities = 252/252 (100%)
 Strand = Plus / Plus

                                                                       
Query: 293 tttcaattctttatgaacctaaaattaaagaaagagaaaaaattaaagaaggaccaattg 352
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 293 tttcaattctttatgaacctaaaattaaagaaagagaaaaaattaaagaaggaccaattg 352

                                                                       
Query: 353 atattgatacaaatgaaccaatttattatactccacaatctggttcaactaaaagatcat 412
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 353 atattgatacaaatgaaccaatttattatactccacaatctggttcaactaaaagatcat 412

                                                                       
Query: 413 caagtattttagattatttcaaaagagatgataaagattatcatgatcatgatgataata 472
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 413 caagtattttagattatttcaaaagagatgataaagattatcatgatcatgatgataata 472

                                                                       
Query: 473 aaccaaccactacaactacaacaacatcatcatcattattatcaccatcatcatttgatg 532
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 473 aaccaaccactacaactacaacaacatcatcatcattattatcaccatcatcatttgatg 532

                       
Query: 533 aggatgattttt 544
           ||||||||||||
Sbjct: 533 aggatgattttt 544


 Score =  385 bits (194), Expect = e-107
 Identities = 194/194 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   ttaagtgatagtgatgatgaagaaattgtaatgaagaaaccaagaagagtttcaacacca 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   ttaagtgatagtgatgatgaagaaattgtaatgaagaaaccaagaagagtttcaacacca 60

                                                                       
Query: 61  agaaagaagagaactagaggtcaattctttatggatagagataaaagtgatgaggaaaga 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  agaaagaagagaactagaggtcaattctttatggatagagataaaagtgatgaggaaaga 120

                                                                       
Query: 121 ggtgatgatacctatcaaattgatgattttgtagttgagaatggttcagatgttgatggt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 ggtgatgatacctatcaaattgatgattttgtagttgagaatggttcagatgttgatggt 180

                         
Query: 181 tcaagtgaagaaga 194
           ||||||||||||||
Sbjct: 181 tcaagtgaagaaga 194


 Score = 30.2 bits (15), Expect = 5.7
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                          
Query: 252 ggataatgaggaggg 266
           |||||||||||||||
Sbjct: 252 ggataatgaggaggg 266


>Contig-U01481-1 (Contig-U01481-1Q) /CSM_Contig/Contig-U01481-1Q.Seq.d
          Length = 1101

 Score = 48.1 bits (24), Expect = 2e-05
 Identities = 33/36 (91%)
 Strand = Plus / Plus

                                               
Query: 484 acaactacaacaacatcatcatcattattatcacca 519
           ||||| ||||||||||||||||||| || |||||||
Sbjct: 64  acaacaacaacaacatcatcatcatcatcatcacca 99


>Contig-U01120-1 (Contig-U01120-1Q) /CSM_Contig/Contig-U01120-1Q.Seq.d
          Length = 1718

 Score = 48.1 bits (24), Expect = 2e-05
 Identities = 27/28 (96%)
 Strand = Plus / Plus

                                       
Query: 481 actacaactacaacaacatcatcatcat 508
           ||||||||||||||| ||||||||||||
Sbjct: 550 actacaactacaacatcatcatcatcat 577


 Score = 44.1 bits (22), Expect = 4e-04
 Identities = 37/42 (88%)
 Strand = Plus / Plus

                                                     
Query: 481 actacaactacaacaacatcatcatcattattatcaccatca 522
           |||||||||||||| ||| ||||||||| || |||| |||||
Sbjct: 544 actacaactacaactacaacatcatcatcatcatcatcatca 585


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 24,015
Number of Sequences: 6905
Number of extensions: 24015
Number of successful extensions: 3704
Number of sequences better than 10.0: 607
length of query: 1296
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1280
effective length of database: 5,564,391
effective search space: 7122420480
effective search space used: 7122420480
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.13
Homology vs DNA 
Query= Contig-U08712-1 (Contig-U08712-1Q) /CSM_Contig/Contig-U08712-1Q.Seq.d
         (1296 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ341813) Dictyostelium discoideum cDNA clone:dda8d09, 3' e...  1340   0.0    1
(AC116925) Dictyostelium discoideum chromosome 2 map 4189423...  1158   0.0    3
(BJ324831) Dictyostelium discoideum cDNA clone:dda8d09, 5' e...   500   0.0    2
(AC116982) Dictyostelium discoideum chromosome 2 map 3622643...    64   1e-05  1
(BJ365925) Dictyostelium discoideum cDNA clone:ddc37g04, 5' ...    64   1e-05  1
(BI515818) BB160020B10D07.5 Bee Brain Normalized Library, BB...    64   1e-05  1
(BI515326) BB160019A10D10.5 Bee Brain Normalized Library, BB...    64   1e-05  1
(BI515312) BB160019A10C06.5 Bee Brain Normalized Library, BB...    64   1e-05  1
(BI510450) BB170023A20B05.5 Bee Brain Normalized/Subtracted ...    64   1e-05  1
(BI508266) BB170023A10C07.5 Bee Brain Normalized/Subtracted ...    64   1e-05  1
(BI505787) BB170020B10H07.5 Bee Brain Normalized/Subtracted ...    64   1e-05  1
(CR382399) Plasmodium falciparum chromosome 6, complete sequ...    62   6e-05  1
(AC113682) Rattus norvegicus clone CH230-235A15, WORKING DRA...    62   6e-05  1
(AC007190) Genomic sequence for Arabidopsis thaliana BAC F23...    60   2e-04  1
(AL157813) Human DNA sequence from clone RP11-165D7 on chrom...    60   2e-04  1
(EI780304) CHORI518002E17TF BAC library from the breast cell...    60   2e-04  1
(EB356504) MCCN24-F-014525-501 Normalized MCC Processes 2 Ap...    60   2e-04  1
(EB355581) R20017-F-024841-501 Non-Normalized CNS library Ap...    60   2e-04  1
(EB349680) CNSN01-F-109957-501 Normalized CNS library (juven...    60   2e-04  1
(EB346221) CNSN01-F-062962-501 Normalized CNS library (juven...    60   2e-04  1
(EB334597) CNSN01-F-141502-501 Normalized CNS library (juven...    60   2e-04  1
(EB328727) CNSN01-F-108429-501 Normalized CNS library (juven...    60   2e-04  1
(EB254332) CNSN01-C-006585-501 Normalized CNS library (juven...    60   2e-04  1
(EB251121) CNSN01-C-010484-501 Normalized CNS library (juven...    60   2e-04  1
(EB243483) PEG003-C-220766-501 Normalized Pedal-Pleural Gang...    60   2e-04  1
(EB194962) PEG001-C-002142-301 Non-Normalized Pedal-Pleural ...    60   2e-04  1
(AC115604) Dictyostelium discoideum chromosome 2 map 4354771...    36   5e-04  7
(AC178032) Strongylocentrotus purpuratus clone R3-1117F2, WO...    52   5e-04  5
(AC168415) Strongylocentrotus purpuratus clone R3-4002B23, W...    44   7e-04  2
(AC184423) Strongylocentrotus purpuratus clone R3-3114C2, WO...    40   7e-04  2
(AC178687) Strongylocentrotus purpuratus clone R3-12D6, WORK...    40   7e-04  2
(AC157973) Strongylocentrotus purpuratus clone R3-4002B23, W...    44   7e-04  2
(BJ401228) Dictyostelium discoideum cDNA clone:dds22f15, 3' ...    56   8e-04  2
(AC158625) Mus musculus 6 BAC RP23-78J15 (Roswell Park Cance...    58   9e-04  1
(AF083690) Arabidopsis thaliana clone sps139 unknown mRNA.         58   9e-04  1
(AX571885) Sequence 3 from Patent WO02066644.                      58   9e-04  1
(AY428796) Dictyostelium discoideum DIF insensitive mutant A...    58   9e-04  1
(AY160102) Dictyostelium discoideum nucleotide exchange fact...    58   9e-04  1
(AF057019) Dictyostelium discoideum interaptin (abpD) gene, ...    58   9e-04  1
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    58   9e-04  1
(DQ217942) Homo sapiens EGF-like module containing mucin-lik...    58   9e-04  1
(AC020895) Homo sapiens chromosome 19 clone CTC-312O10, comp...    58   9e-04  1
(AC160867) Mus musculus clone RP24-93A14, WORKING DRAFT SEQU...    58   9e-04  1
(AC114201) Rattus norvegicus clone CH230-215N8, *** SEQUENCI...    58   9e-04  1
(AC068358) Homo sapiens chromosome 8 clone RP11-113I18 map 8...    58   9e-04  1
(AC027726) Homo sapiens clone RP11-815E8, WORKING DRAFT SEQU...    58   9e-04  1
(AC181533) Strongylocentrotus purpuratus clone R3-7C11, WORK...    58   9e-04  1
(AC180349) Strongylocentrotus purpuratus clone R3-3116O19, W...    58   9e-04  1
(AC168625) Strongylocentrotus purpuratus clone R3-3046C3, WO...    58   9e-04  1
(EB270452) MCC013-F-016981-501 Non-Normalized MCC Processes ...    58   9e-04  1
(BJ392227) Dictyostelium discoideum cDNA clone:dds26j20, 5' ...    58   9e-04  1
(BJ366181) Dictyostelium discoideum cDNA clone:ddc38f03, 5' ...    58   9e-04  1
(EY490151) CBBP17479.rev CBBP Hirudo medicinalis hermaphrodi...    58   9e-04  1
(BJ345428) Dictyostelium discoideum cDNA clone:dda23m13, 3' ...    56   0.001  2
(BJ389363) Dictyostelium discoideum cDNA clone:dds18p10, 5' ...    56   0.001  2
(AC178780) Strongylocentrotus purpuratus clone R3-21D19, WOR...    44   0.001  7
(DY827635) CTOY3373.b1_J04.ab1 CTO(XYZ) dandelion Taraxacum ...    52   0.001  2
(AC181251) Strongylocentrotus purpuratus clone R3-4010O01, W...    46   0.002  6
(AC115606) Dictyostelium discoideum chromosome 2 map 159760-...    40   0.002  6
(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...    34   0.002  13
(AC178846) Strongylocentrotus purpuratus clone R3-26F6, WORK...    40   0.002  2
(AC176576) Strongylocentrotus purpuratus clone R3-3077E11, W...    40   0.002  2
(AC160127) Mus musculus BAC clone RP23-295O2 from chromosome...    38   0.002  2
(AC176159) Strongylocentrotus purpuratus clone R3-3019G08, W...    40   0.002  2
(AC184509) Strongylocentrotus purpuratus clone R3-1105H10, W...    46   0.002  2
(AC180777) Strongylocentrotus purpuratus clone R3-3075I13, W...    38   0.002  2
(AC201016) Strongylocentrotus purpuratus clone R3-12H10, WOR...    40   0.003  2
(EH772209) CNSM4754.b1_D13.ab1 CNS(LMS) yellow starthistle C...    42   0.003  2
(DQ889019) Paralichthys olivaceus microsatellite Poli1455TUF...    56   0.003  1
(AC142071) Rattus norvegicus clone CH230-82F20, *** SEQUENCI...    56   0.003  1
(AC122657) Rattus norvegicus clone CH230-23G10, *** SEQUENCI...    56   0.003  1
(AC094421) Rattus norvegicus clone CH230-3K13, *** SEQUENCIN...    56   0.003  1
(AC178993) Strongylocentrotus purpuratus clone R3-3122L9, WO...    56   0.003  1
(AC178213) Strongylocentrotus purpuratus clone R3-3085F20, W...    56   0.003  1
(AC176172) Strongylocentrotus purpuratus clone R3-3066I12, W...    56   0.003  1
(EF750587) Mus musculus C57BL/6N IST10523A2HMF1 gene trap em...    56   0.003  1
(AU034157) Dictyostelium discoideum slug cDNA, clone SLC113.       56   0.003  1
(BJ409354) Dictyostelium discoideum cDNA clone:dds41f03, 3' ...    56   0.003  1
(AE014820) Plasmodium falciparum 3D7 chromosome 14 section 5...    54   0.006  12
(AC178556) Strongylocentrotus purpuratus clone R3-3027K04, W...    48   0.006  7
(AC146745) Medicago truncatula clone mth2-53p19, complete se...    36   0.006  10
(AC177110) Strongylocentrotus purpuratus clone R3-4014C19, W...    48   0.006  7
(AC178183) Strongylocentrotus purpuratus clone R3-3082D3, WO...    50   0.007  5
(AC112873) Rattus norvegicus clone CH230-127E20, *** SEQUENC...    52   0.007  5
(AC178593) Strongylocentrotus purpuratus clone R3-3021M6, WO...    50   0.007  5
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    46   0.008  16
(AC133858) Rattus norvegicus clone CH230-3F10, *** SEQUENCIN...    38   0.009  2
(AC114472) Rattus norvegicus clone CH230-16P2, WORKING DRAFT...    38   0.009  2
(AC139831) Homo sapiens chromosome 5 clone RP11-770A9, WORKI...    38   0.009  2
(AC180902) Strongylocentrotus purpuratus clone R3-3064G16, W...    44   0.009  2
(AC091579) Homo sapiens clone RP11-962O23, WORKING DRAFT SEQ...    36   0.009  2
(AC139817) Homo sapiens chromosome 5 clone RP11-1400O12, WOR...    38   0.009  2
(AC184647) Strongylocentrotus purpuratus clone R3-3043F14, W...    44   0.009  2
(AC176814) Strongylocentrotus purpuratus clone R3-3111B06, W...    44   0.009  2
(AC178854) Strongylocentrotus purpuratus clone R3-28N2, WORK...    52   0.009  2
(AC139813) Homo sapiens chromosome 5 clone RP11-1382L11, WOR...    38   0.009  2
(AC177725) Strongylocentrotus purpuratus clone R3-1008P11, W...    44   0.009  2
(AC140523) Homo sapiens chromosome 5 clone RP11-840B15, WORK...    38   0.009  2
(AC141608) Homo sapiens chromosome 5 clone RP11-690D5, WORKI...    38   0.009  2
(AC149995) Strongylocentrotus purpuratus clone R3-60J17, WOR...    44   0.009  2

>(BJ341813) Dictyostelium discoideum cDNA clone:dda8d09, 3' end,
            single read.
          Length = 677

 Score = 1340 bits (676), Expect = 0.0
 Identities = 676/676 (100%)
 Strand = Plus / Minus


Query: 621  ctcatcaagatccaaattttattaaagaaaaagtattaaaagagtttttctaaaaagttt 680
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 677  ctcatcaagatccaaattttattaaagaaaaagtattaaaagagtttttctaaaaagttt 618


Query: 681  accattaaagaatcattttcaatctttattcaaatatgtaattagttgtattgttgatga 740
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 617  accattaaagaatcattttcaatctttattcaaatatgtaattagttgtattgttgatga 558


Query: 741  agagttttcaagagcagtgattgaagataaggatgaatatttcttggatgcaattaatag 800
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 557  agagttttcaagagcagtgattgaagataaggatgaatatttcttggatgcaattaatag 498


Query: 801  agttaaaagttttatcgttagtaaaaaggaagcattggtattgggtaatatgtggcatga 860
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 497  agttaaaagttttatcgttagtaaaaaggaagcattggtattgggtaatatgtggcatga 438


Query: 861  tgaatatttacatgatatcacacatagaccatttcttcattctgccgaagtttcagaata 920
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 437  tgaatatttacatgatatcacacatagaccatttcttcattctgccgaagtttcagaata 378


Query: 921  tgattatgaaggtagaaaatgtaaatcatgtaataaagccaaacataatgttaccatcat 980
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 377  tgattatgaaggtagaaaatgtaaatcatgtaataaagccaaacataatgttaccatcat 318


Query: 981  tatctatttctctggtagatcctatgatgtcgatgatttttggagtggttattttgaaaa 1040
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 317  tatctatttctctggtagatcctatgatgtcgatgatttttggagtggttattttgaaaa 258


Query: 1041 tccaaaacgtttaaaaggtaccaccaaagatgaaccaaaatcaatcgaatatgaattggg 1100
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 257  tccaaaacgtttaaaaggtaccaccaaagatgaaccaaaatcaatcgaatatgaattggg 198


Query: 1101 ttcattctgtttcaaacgttcacaactttttcatcaactccatcattttccttataaatt 1160
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 197  ttcattctgtttcaaacgttcacaactttttcatcaactccatcattttccttataaatt 138


Query: 1161 ctttagagatgttaaacaagatgttagagatttacaacaacttcattcggattggacaaa 1220
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 137  ctttagagatgttaaacaagatgttagagatttacaacaacttcattcggattggacaaa 78


Query: 1221 tcaagatattctttttcatatgacctctccagataatagttatgtttcaagctattttaa 1280
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 77   tcaagatattctttttcatatgacctctccagataatagttatgtttcaagctattttaa 18


Query: 1281 ctttttagaaaattta 1296
            ||||||||||||||||
Sbjct: 17   ctttttagaaaattta 2

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,559,110,278
Number of extensions: 101961975
Number of successful extensions: 9434199
Number of sequences better than 10.0: 2444
Length of query: 1296
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1272
Effective length of database: 97,308,875,965
Effective search space: 123776890227480
Effective search space used: 123776890227480
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.24
Homology vs Protein 
Query= Contig-U08712-1 (Contig-U08712-1Q) /CSM_Contig/Contig-U08712-1Q.Seq.d
         (1296 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116925_14(AC116925|pid:none) Dictyostelium discoideum chromoso...   418   e-115
(O13803) Uncharacterized protein C17H9.06c.  &CU329670_824(CU329...    59   4e-07
AE014296_3499(AE014296|pid:none) Drosophila melanogaster chromos...    55   5e-06
(Q2KHR3) RecName: Full=Glutamine and serine-rich protein 1;            44   0.013
BX908741_3(BX908741|pid:none) Mouse DNA sequence from clone RP23...    43   0.022
BC054711_1(BC054711|pid:none) Danio rerio zgc:66477, mRNA (cDNA ...    41   0.082
BC112935_1(BC112935|pid:none) Homo sapiens glutamine and serine ...    40   0.18
AK314669_1(AK314669|pid:none) Homo sapiens cDNA, FLJ95516.             40   0.24
(Q8N4S0) RecName: Full=Coiled-coil domain-containing protein 82;...    40   0.24
AK027171_1(AK027171|pid:none) Homo sapiens cDNA: FLJ23518 fis, c...    40   0.24
AK056790_1(AK056790|pid:none) Homo sapiens cDNA FLJ32228 fis, cl...    40   0.24
BC158914_1(BC158914|pid:none) Xenopus tropicalis hypothetical pr...    39   0.41
(Q9STN3) RecName: Full=Putative transcription elongation factor ...    39   0.53
BC081280_1(BC081280|pid:none) Xenopus laevis Glutamine and serin...    39   0.53
(Q66H73) RecName: Full=Coiled-coil domain-containing protein 82;...    38   0.70
AM946015_317(AM946015|pid:none) Streptococcus uberis 0140J compl...    36   2.6
DQ077553_25(DQ077553|pid:none) Uncultured bacterium MedeBAC35C06...    35   5.9
CP000932_347(CP000932|pid:none) Campylobacter lari RM2100, compl...    35   7.7

>AC116925_14(AC116925|pid:none) Dictyostelium discoideum chromosome 2
            map 4189423-4228998 strain AX4, complete sequence.
          Length = 1404

 Score =  418 bits (1075), Expect = e-115
 Identities = 198/210 (94%), Positives = 200/210 (95%)
 Frame = +1

Query: 667  FLKSLPLKNHFQSLFKYVISCIVDEEFSRAVIEDKDEYFLDAINRVKSFIVSKKEALVLG 846
            F K   +K  F    +YVISCIVDEEFSRAVIEDKDEYFLDAINRVKSFIVSKKEALVLG
Sbjct: 1184 FSKKFTIKESFSIFIQYVISCIVDEEFSRAVIEDKDEYFLDAINRVKSFIVSKKEALVLG 1243

Query: 847  NMWHDEYLHDITHRPFLHSAEVSEYDYEGRKCKSCNKAKHNVTIIIYFSGRSYDVDDFWS 1026
            NMWHDEYLHDITHRPFLHSAEVSEYDYEGRKCKSCNKAKHNVTIIIYFSGRSYDVDDFWS
Sbjct: 1244 NMWHDEYLHDITHRPFLHSAEVSEYDYEGRKCKSCNKAKHNVTIIIYFSGRSYDVDDFWS 1303

Query: 1027 GYFENPKRLKGTTKDEPKSIEYELGSFCFKRSQLFHQLHHFPYKFFRDVKQDVRDLQQLH 1206
            GYFENPKRLKGTTKDEPKSIEYELGSFCFKRSQLFHQLHHFPYKFFRDVKQDVRDLQQLH
Sbjct: 1304 GYFENPKRLKGTTKDEPKSIEYELGSFCFKRSQLFHQLHHFPYKFFRDVKQDVRDLQQLH 1363

Query: 1207 SDWTNQDILFHMTSPDNSYVSSYFNFLENL 1296
            SDWTNQDILFHMTSPDNSYVSSYFNFLENL
Sbjct: 1364 SDWTNQDILFHMTSPDNSYVSSYFNFLENL 1393

 Score =  164 bits (414), Expect = 8e-39
 Identities = 90/141 (63%), Positives = 90/141 (63%)
 Frame = +1

Query: 1    LSDSDDEEIVMKKPRRVSTPRKKRTRGQFFMDRDKSDEERGDDTYQIDDFVVENGSDVDG 180
            LSDSDDEEIVMKKPRRVSTPRKKRTRGQFFMDRDKSDEERGDDTYQIDDFVVENGSDVDG
Sbjct: 969  LSDSDDEEIVMKKPRRVSTPRKKRTRGQFFMDRDKSDEERGDDTYQIDDFVVENGSDVDG 1028

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXNXXXXXXXXXXXVSILYXXXXXXXXXXXXXXXDID 360
                                     N           VSILY               DID
Sbjct: 1029 SSEEEELKRKSRRRDRKIIINDEKDNEEGEEEEEEEEVSILYEPKIKEREKIKEGPIDID 1088

Query: 361  TNEPIYYTPQSGSTKRSSSIL 423
            TNEPIYYTPQSGSTKRSSSIL
Sbjct: 1089 TNEPIYYTPQSGSTKRSSSIL 1109

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,316,529,057
Number of extensions: 23086615
Number of successful extensions: 66508
Number of sequences better than 10.0: 18
Number of HSP's gapped: 66416
Number of HSP's successfully gapped: 19
Length of query: 432
Length of database: 1,040,966,779
Length adjustment: 131
Effective length of query: 301
Effective length of database: 621,205,444
Effective search space: 186982838644
Effective search space used: 186982838644
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 1.02  gvh: 0.38  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for Contig-U08712-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0