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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U08500-1
Contig ID Contig-U08500-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U08500-1 (Contig-U08500-1Q) /CSM_Contig/Contig-U08500-1Q.Seq.d
NNNNNNNNNNACCCACCACCACCACCACAAATTTGCGGTAGTGTAAATTG
TGGTCCAGGTTACATTTGTACAATTATCAATGGTCATCCAACTTGTATTC
GTGGTGATGGTTATTTATGTAATCAAACCCGTTGTCCACACGATTATCAA
TGTGAAACCATTAGCACCAATATCGTGAAATGTTCACCAAAGAATGACGA
ATGTAAATGGCATCGTTGTCCACCAGGTTCCAGCATGTAATCACGTTGGA
ATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCATG
TTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAA
CCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACT
GCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAG
TAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAA
GCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCT
TCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCTC
AAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTG
CAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTC
Gap no gap
Contig length 687
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 415398
End point 416075
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U08500
List of clone(s) 

est1=CFH866Z,1,678
Translated Amino Acid sequence 
xxxthhhhhkfavv*ivvqvtfvqlsmviqlvfvvmviyvikpvvhtiinvkplapis*N
VHQRMTNVNGIVVHQVPACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAASTIAT
TASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAASSAASSEPSS
SAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSAT
Translated Amino Acid sequence (All Frames) 
Frame A:
xxxxpppppqicgsvncgpgyictiinghptcirgdgylcnqtrcphdyqcetistnivk
cspkndeckwhrcppgssm*srwndlysstkqlyqy*ipmlpissnlyssinyccfnhcn
ncincrnynlcnycrynnwwnynwwfnf***ccflsr**rcllitikqccfkcrfk*tik
*rrfk*csilcfk*rsilclk*csiiisfkfsciiscklrklrklishf


Frame B:
xxxthhhhhkfavv*ivvqvtfvqlsmviqlvfvvmviyvikpvvhtiinvkplapis*N
VHQRMTNVNGIVVHQVPACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAASTIAT
TASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAASSAASSEPSS
SAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSAT


Frame C:
xxxptttttnlr*cklwsrlhlynyqwssnlysw*wlfm*snplstrlsm*nh*hqyrem
ftke*rm*maslstrfqhvitle*fvfkyqaivpildshvahliqfvfihqllllqplqq
lhqlsqlqplqllqvqqlvelqlvvqllivvllpqpivalpphhhqavllqvplqvnhqv
aplqvvlhpllqvalhplpqvvlhhhqlqvqlhhqlqaqkaqkahqpl
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U08500-1 (Contig-U08500-1Q)
/CSM_Contig/Contig-U08500-1Q.Seq.d
         (687 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08500-1 (Contig-U08500-1Q) /CSM_Contig/Conti...  1342  0.0
Contig-U00750-1 (Contig-U00750-1Q) /CSM_Contig/Conti...   878  0.0
Contig-U04204-1 (Contig-U04204-1Q) /CSM_Contig/Conti...   652  0.0
Contig-U02514-1 (Contig-U02514-1Q) /CSM_Contig/Conti...   638  0.0
Contig-U00301-1 (Contig-U00301-1Q) /CSM_Contig/Conti...    54  2e-07
Contig-U12585-1 (Contig-U12585-1Q) /CSM_Contig/Conti...    40  0.003
Contig-U11110-1 (Contig-U11110-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U01028-1 (Contig-U01028-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U14742-1 (Contig-U14742-1Q) /CSM_Contig/Conti...    36  0.048
Contig-U13509-1 (Contig-U13509-1Q) /CSM_Contig/Conti...    36  0.048

>Contig-U08500-1 (Contig-U08500-1Q) /CSM_Contig/Contig-U08500-1Q.Seq.d
          Length = 687

 Score = 1342 bits (677), Expect = 0.0
 Identities = 677/677 (100%)
 Strand = Plus / Plus

                                                                       
Query: 11  acccaccaccaccaccacaaatttgcggtagtgtaaattgtggtccaggttacatttgta 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 11  acccaccaccaccaccacaaatttgcggtagtgtaaattgtggtccaggttacatttgta 70

                                                                       
Query: 71  caattatcaatggtcatccaacttgtattcgtggtgatggttatttatgtaatcaaaccc 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71  caattatcaatggtcatccaacttgtattcgtggtgatggttatttatgtaatcaaaccc 130

                                                                       
Query: 131 gttgtccacacgattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaa 190
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 131 gttgtccacacgattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaa 190

                                                                       
Query: 191 agaatgacgaatgtaaatggcatcgttgtccaccaggttccagcatgtaatcacgttgga 250
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 191 agaatgacgaatgtaaatggcatcgttgtccaccaggttccagcatgtaatcacgttgga 250

                                                                       
Query: 251 atgatttgtattcaagtaccaagcaattgtaccaatactagattcccatgttgcccatct 310
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 251 atgatttgtattcaagtaccaagcaattgtaccaatactagattcccatgttgcccatct 310

                                                                       
Query: 311 catccaatttgtattcatccatcaactactgctgcttcaaccattgcaacaactgcatca 370
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 311 catccaatttgtattcatccatcaactactgctgcttcaaccattgcaacaactgcatca 370

                                                                       
Query: 371 actgtcgcaactacaacctctgcaactactgcaggtacaacaactggtggaactacaact 430
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 371 actgtcgcaactacaacctctgcaactactgcaggtacaacaactggtggaactacaact 430

                                                                       
Query: 431 ggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagcgctgcctcctca 490
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 431 ggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagcgctgcctcctca 490

                                                                       
Query: 491 tcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatcaagtagcgccgct 550
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 491 tcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatcaagtagcgccgct 550

                                                                       
Query: 551 tcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcctcaagtagtgct 610
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 551 tcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcctcaagtagtgct 610

                                                                       
Query: 611 ccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctcagaaagctcagaa 670
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 611 ccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctcagaaagctcagaa 670

                            
Query: 671 agctcatcagccacttc 687
           |||||||||||||||||
Sbjct: 671 agctcatcagccacttc 687


>Contig-U00750-1 (Contig-U00750-1Q) /CSM_Contig/Contig-U00750-1Q.Seq.d
          Length = 761

 Score =  878 bits (443), Expect = 0.0
 Identities = 457/459 (99%), Gaps = 2/459 (0%)
 Strand = Plus / Plus

                                                                       
Query: 231 cagcatgtaatcacgttggaatgatttgtattcaagtaccaagcaattgtaccaatacta 290
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 302 cagcatgtaatcacgttggaatgatttgtattcaagtaccaagcaattgtaccaatacta 361

                                                                       
Query: 291 gattcccatgttgcccatctcatccaatttgtattcatccatcaactactgctgcttcaa 350
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 362 gattcccatgttgcccatctcatccaatttgtattcatccatcaactactgctgcttcaa 421

                                                                       
Query: 351 ccattgcaacaactgcatcaactgtcgcaactacaacctctgcaactactgcaggtacaa 410
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 422 ccattgcaacaactgcatcaactgtcgcaactacaacctctgcaactactgcaggtacaa 481

                                                                       
Query: 411 caactggtggaactacaactggtggttcaacttctgatagtagtgctgcttcctcagccg 470
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 482 caactggtggaactacaactggtggttcaacttctgatagtagtgctgcttcctcagccg 541

                                                                       
Query: 471 atagtagcgctgcctcctcatcaccatcaagcagtgctgcttcaagtgccgcttcaagtg 530
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 542 atagtagcgctgcctcctcatcaccatcaagcagtgctgcttcaagtgccgcttcaagtg 601

                                                                       
Query: 531 aaccatcaagtagcgccgcttcaagtagtgctccatcctctgcttcaagtagcgctccat 590
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 602 aaccatcaagtagcgccgcttcaagtagtgctccatcctctgcttcaagtagcgctccat 661

                                                                       
Query: 591 cctctgcctcaagtagtgctccatcatcatcagcttcaagttcagctgcatcatcagctg 650
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 662 cctctgcctcaagtagtgctccatcatcatcagcttcaagttcagctgcatcatcagctg 721

                                                  
Query: 651 caag-ctcagaaag-ctcagaaagctcatcagccacttc 687
           |||| ||||||||| ||||||||||||||||||||||||
Sbjct: 722 caagnctcagaaagnctcagaaagctcatcagccacttc 760


 Score =  295 bits (149), Expect = 3e-80
 Identities = 152/153 (99%)
 Strand = Plus / Plus

                                                                       
Query: 82  ggtcatccaacttgtattcgtggtgatggttatttatgtaatcaaacccgttgtccacac 141
           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct: 1   ggtcatccaacttgtattcgtggtgatggttatttatgtgatcaaacccgttgtccacac 60

                                                                       
Query: 142 gattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaaagaatgacgaa 201
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  gattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaaagaatgacgaa 120

                                            
Query: 202 tgtaaatggcatcgttgtccaccaggttccagc 234
           |||||||||||||||||||||||||||||||||
Sbjct: 121 tgtaaatggcatcgttgtccaccaggttccagc 153


>Contig-U04204-1 (Contig-U04204-1Q) /CSM_Contig/Contig-U04204-1Q.Seq.d
          Length = 448

 Score =  652 bits (329), Expect = 0.0
 Identities = 329/329 (100%)
 Strand = Plus / Plus

                                                                       
Query: 359 acaactgcatcaactgtcgcaactacaacctctgcaactactgcaggtacaacaactggt 418
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 91  acaactgcatcaactgtcgcaactacaacctctgcaactactgcaggtacaacaactggt 150

                                                                       
Query: 419 ggaactacaactggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagc 478
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 151 ggaactacaactggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagc 210

                                                                       
Query: 479 gctgcctcctcatcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatca 538
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 211 gctgcctcctcatcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatca 270

                                                                       
Query: 539 agtagcgccgcttcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcc 598
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 271 agtagcgccgcttcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcc 330

                                                                       
Query: 599 tcaagtagtgctccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctca 658
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 331 tcaagtagtgctccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctca 390

                                        
Query: 659 gaaagctcagaaagctcatcagccacttc 687
           |||||||||||||||||||||||||||||
Sbjct: 391 gaaagctcagaaagctcatcagccacttc 419


 Score = 75.8 bits (38), Expect = 6e-14
 Identities = 38/38 (100%)
 Strand = Plus / Minus

                                                 
Query: 277 ttgtaccaatactagattcccatgttgcccatctcatc 314
           ||||||||||||||||||||||||||||||||||||||
Sbjct: 94  ttgtaccaatactagattcccatgttgcccatctcatc 57


 Score = 69.9 bits (35), Expect = 3e-12
 Identities = 35/35 (100%)
 Strand = Plus / Minus

                                              
Query: 366 catcaactgtcgcaactacaacctctgcaactact 400
           |||||||||||||||||||||||||||||||||||
Sbjct: 60  catcaactgtcgcaactacaacctctgcaactact 26


 Score = 61.9 bits (31), Expect = 8e-10
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 577 aagtagcgctccatcctctgcctcaagtagt 607
           |||||||||||||||||||||||||||||||
Sbjct: 1   aagtagcgctccatcctctgcctcaagtagt 31


 Score = 44.1 bits (22), Expect = 2e-04
 Identities = 28/30 (93%)
 Strand = Plus / Plus

                                         
Query: 553 aagtagtgctccatcctctgcttcaagtag 582
           |||||| |||||||||||||| ||||||||
Sbjct: 1   aagtagcgctccatcctctgcctcaagtag 30


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8036
Number of Sequences: 6905
Number of extensions: 8036
Number of successful extensions: 1369
Number of sequences better than 10.0: 216
length of query: 687
length of database: 5,674,871
effective HSP length: 16
effective length of query: 671
effective length of database: 5,564,391
effective search space: 3733706361
effective search space used: 3733706361
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.13
Homology vs DNA 
Query= Contig-U08500-1 (Contig-U08500-1Q) /CSM_Contig/Contig-U08500-1Q.Seq.d
         (687 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ374854) Dictyostelium discoideum cDNA clone:ddc16d18, 3' ...  1342   0.0    1
(D13973) Dictyostelium discoideum gene for Dp87 protein, com...   906   0.0    2
(AU052205) Dictyostelium discoideum slug cDNA, clone SLA236.      906   0.0    2
(AU033627) Dictyostelium discoideum slug cDNA, clone SLB244.      906   0.0    2
(AU033525) Dictyostelium discoideum slug cDNA, clone SLB132.      906   0.0    2
(BJ403598) Dictyostelium discoideum cDNA clone:dds25l16, 3' ...   906   0.0    2
(BJ402864) Dictyostelium discoideum cDNA clone:dds18c13, 3' ...   906   0.0    2
(BJ402820) Dictyostelium discoideum cDNA clone:dds18j08, 3' ...   906   0.0    2
(BJ402817) Dictyostelium discoideum cDNA clone:dds18i11, 3' ...   906   0.0    2
(BJ402470) Dictyostelium discoideum cDNA clone:dds17e02, 3' ...   906   0.0    2
(BJ401618) Dictyostelium discoideum cDNA clone:dds23i21, 3' ...   906   0.0    2
(BJ401532) Dictyostelium discoideum cDNA clone:dds23h14, 3' ...   906   0.0    3
(BJ401236) Dictyostelium discoideum cDNA clone:dds22h13, 3' ...   906   0.0    2
(BJ401106) Dictyostelium discoideum cDNA clone:dds22l05, 3' ...   906   0.0    2
(BJ400069) Dictyostelium discoideum cDNA clone:dds8o18, 3' e...   906   0.0    2
(BJ399871) Dictyostelium discoideum cDNA clone:dds7g23, 3' e...   906   0.0    2
(BJ399313) Dictyostelium discoideum cDNA clone:dds5k12, 3' e...   906   0.0    2
(BJ398428) Dictyostelium discoideum cDNA clone:dds12m08, 3' ...   906   0.0    2
(BJ398316) Dictyostelium discoideum cDNA clone:dds11p21, 3' ...   906   0.0    3
(BJ398027) Dictyostelium discoideum cDNA clone:dds10m16, 3' ...   906   0.0    2
(BJ397889) Dictyostelium discoideum cDNA clone:dds10e02, 3' ...   906   0.0    2
(BJ385512) Dictyostelium discoideum cDNA clone:ddc55o03, 3' ...   906   0.0    2
(BJ384625) Dictyostelium discoideum cDNA clone:ddc50l01, 3' ...   906   0.0    2
(BJ376434) Dictyostelium discoideum cDNA clone:ddc28c13, 3' ...   906   0.0    2
(BJ376161) Dictyostelium discoideum cDNA clone:ddc21n11, 3' ...   906   0.0    2
(BJ376122) Dictyostelium discoideum cDNA clone:ddc21c11, 3' ...   906   0.0    2
(BJ375408) Dictyostelium discoideum cDNA clone:ddc18j13, 3' ...   906   0.0    2
(BJ375386) Dictyostelium discoideum cDNA clone:ddc18d15, 3' ...   906   0.0    2
(BJ375328) Dictyostelium discoideum cDNA clone:ddc18e12, 3' ...   906   0.0    2
(BJ375314) Dictyostelium discoideum cDNA clone:ddc18c07, 3' ...   906   0.0    2
(BJ375275) Dictyostelium discoideum cDNA clone:ddc18i04, 3' ...   906   0.0    2
(BJ374251) Dictyostelium discoideum cDNA clone:ddc6l05, 3' e...   906   0.0    2
(BJ374022) Dictyostelium discoideum cDNA clone:ddc5l24, 3' e...   906   0.0    2
(BJ372604) Dictyostelium discoideum cDNA clone:ddc13f10, 3' ...   906   0.0    2
(BJ372534) Dictyostelium discoideum cDNA clone:ddc13d04, 3' ...   906   0.0    2
(BJ372396) Dictyostelium discoideum cDNA clone:ddc12p10, 3' ...   906   0.0    2
(BJ372248) Dictyostelium discoideum cDNA clone:ddc11h22, 3' ...   906   0.0    2
(BJ372231) Dictyostelium discoideum cDNA clone:ddc11d22, 3' ...   906   0.0    2
(BJ372130) Dictyostelium discoideum cDNA clone:ddc11l11, 3' ...   906   0.0    3
(BJ372028) Dictyostelium discoideum cDNA clone:ddc11a04, 3' ...   906   0.0    2
(BJ356372) Dictyostelium discoideum cDNA clone:dda60i05, 3' ...   906   0.0    2
(BJ352274) Dictyostelium discoideum cDNA clone:dda46o06, 3' ...   906   0.0    3
(BJ351081) Dictyostelium discoideum cDNA clone:dda42a06, 3' ...   906   0.0    2
(BJ347613) Dictyostelium discoideum cDNA clone:dda27j15, 3' ...   906   0.0    2
(BJ347551) Dictyostelium discoideum cDNA clone:dda27n08, 3' ...   906   0.0    2
(BJ347379) Dictyostelium discoideum cDNA clone:dda26n19, 3' ...   906   0.0    2
(BJ347353) Dictyostelium discoideum cDNA clone:dda26i21, 3' ...   906   0.0    2
(BJ347337) Dictyostelium discoideum cDNA clone:dda26f22, 3' ...   906   0.0    2
(BJ347061) Dictyostelium discoideum cDNA clone:dda26b01, 3' ...   906   0.0    2
(BJ346760) Dictyostelium discoideum cDNA clone:dda24o19, 3' ...   906   0.0    2
(BJ346725) Dictyostelium discoideum cDNA clone:dda24g22, 3' ...   906   0.0    2
(BJ346602) Dictyostelium discoideum cDNA clone:dda24o08, 3' ...   906   0.0    3
(BJ346451) Dictyostelium discoideum cDNA clone:dda30p20, 3' ...   906   0.0    2
(BJ346443) Dictyostelium discoideum cDNA clone:dda30m24, 3' ...   906   0.0    2
(BJ346434) Dictyostelium discoideum cDNA clone:dda30k23, 3' ...   906   0.0    2
(BJ346360) Dictyostelium discoideum cDNA clone:dda30m16, 3' ...   906   0.0    2
(BJ346293) Dictyostelium discoideum cDNA clone:dda30p12, 3' ...   906   0.0    2
(BJ346278) Dictyostelium discoideum cDNA clone:dda30n07, 3' ...   906   0.0    2
(BJ346243) Dictyostelium discoideum cDNA clone:dda30g11, 3' ...   906   0.0    2
(BJ346189) Dictyostelium discoideum cDNA clone:dda30k04, 3' ...   906   0.0    2
(BJ346159) Dictyostelium discoideum cDNA clone:dda30e02, 3' ...   906   0.0    3
(BJ346157) Dictyostelium discoideum cDNA clone:dda30d06, 3' ...   906   0.0    2
(BJ345945) Dictyostelium discoideum cDNA clone:dda29g18, 3' ...   906   0.0    2
(BJ345741) Dictyostelium discoideum cDNA clone:dda28m17, 3' ...   906   0.0    2
(BJ345712) Dictyostelium discoideum cDNA clone:dda28g16, 3' ...   906   0.0    3
(BJ345570) Dictyostelium discoideum cDNA clone:dda28i03, 3' ...   906   0.0    2
(BJ345404) Dictyostelium discoideum cDNA clone:dda23h14, 3' ...   906   0.0    2
(BJ345142) Dictyostelium discoideum cDNA clone:dda21b22, 3' ...   906   0.0    2
(BJ345026) Dictyostelium discoideum cDNA clone:dda21i10, 3' ...   906   0.0    2
(BJ344910) Dictyostelium discoideum cDNA clone:dda20n23, 3' ...   906   0.0    2
(BJ344765) Dictyostelium discoideum cDNA clone:dda20m11, 3' ...   906   0.0    2
(BJ344726) Dictyostelium discoideum cDNA clone:dda20e11, 3' ...   906   0.0    2
(BJ344675) Dictyostelium discoideum cDNA clone:dda20j04, 3' ...   906   0.0    2
(BJ344671) Dictyostelium discoideum cDNA clone:dda20i05, 3' ...   906   0.0    2
(BJ344655) Dictyostelium discoideum cDNA clone:dda20e06, 3' ...   906   0.0    2
(BJ344600) Dictyostelium discoideum cDNA clone:dda19j23, 3' ...   906   0.0    3
(BJ344584) Dictyostelium discoideum cDNA clone:dda19g21, 3' ...   906   0.0    2
(BJ344573) Dictyostelium discoideum cDNA clone:dda19d24, 3' ...   906   0.0    2
(BJ344557) Dictyostelium discoideum cDNA clone:dda19a23, 3' ...   906   0.0    3
(BJ344548) Dictyostelium discoideum cDNA clone:dda19o17, 3' ...   906   0.0    2
(BJ344488) Dictyostelium discoideum cDNA clone:dda19b17, 3' ...   906   0.0    3
(BJ344138) Dictyostelium discoideum cDNA clone:dda18e15, 3' ...   906   0.0    2
(BJ344119) Dictyostelium discoideum cDNA clone:dda18a18, 3' ...   906   0.0    3
(BJ343147) Dictyostelium discoideum cDNA clone:dda22n01, 3' ...   906   0.0    2
(BJ342586) Dictyostelium discoideum cDNA clone:dda14p14, 3' ...   906   0.0    3
(BJ342555) Dictyostelium discoideum cDNA clone:dda14g17, 3' ...   906   0.0    2
(BJ341899) Dictyostelium discoideum cDNA clone:dda8k18, 3' e...   906   0.0    2
(BJ341848) Dictyostelium discoideum cDNA clone:dda8n11, 3' e...   906   0.0    2
(BJ341784) Dictyostelium discoideum cDNA clone:dda8l04, 3' e...   906   0.0    2
(BJ341342) Dictyostelium discoideum cDNA clone:dda6b08, 3' e...   906   0.0    2
(BJ339880) Dictyostelium discoideum cDNA clone:dda10c22, 3' ...   906   0.0    2
(BJ346326) Dictyostelium discoideum cDNA clone:dda30g18, 3' ...   904   0.0    2
(BJ371943) Dictyostelium discoideum cDNA clone:ddc10o12, 3' ...   902   0.0    2
(BJ371769) Dictyostelium discoideum cDNA clone:ddc1m08, 3' e...   902   0.0    2
(BJ353763) Dictyostelium discoideum cDNA clone:dda51h12, 3' ...   902   0.0    2
(BJ352602) Dictyostelium discoideum cDNA clone:dda47d10, 3' ...   900   0.0    2
(BJ344689) Dictyostelium discoideum cDNA clone:dda20m05, 3' ...   900   0.0    2
(BJ402643) Dictyostelium discoideum cDNA clone:dds17g16, 3' ...   898   0.0    2
(BJ401154) Dictyostelium discoideum cDNA clone:dds22f12, 3' ...   898   0.0    2
(BJ377440) Dictyostelium discoideum cDNA clone:ddc24g14, 3' ...   898   0.0    3

>(BJ374854) Dictyostelium discoideum cDNA clone:ddc16d18, 3' end,
           single read.
          Length = 678

 Score = 1342 bits (677), Expect = 0.0
 Identities = 677/677 (100%)
 Strand = Plus / Minus


Query: 11  acccaccaccaccaccacaaatttgcggtagtgtaaattgtggtccaggttacatttgta 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 678 acccaccaccaccaccacaaatttgcggtagtgtaaattgtggtccaggttacatttgta 619


Query: 71  caattatcaatggtcatccaacttgtattcgtggtgatggttatttatgtaatcaaaccc 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 618 caattatcaatggtcatccaacttgtattcgtggtgatggttatttatgtaatcaaaccc 559


Query: 131 gttgtccacacgattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaa 190
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 558 gttgtccacacgattatcaatgtgaaaccattagcaccaatatcgtgaaatgttcaccaa 499


Query: 191 agaatgacgaatgtaaatggcatcgttgtccaccaggttccagcatgtaatcacgttgga 250
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 498 agaatgacgaatgtaaatggcatcgttgtccaccaggttccagcatgtaatcacgttgga 439


Query: 251 atgatttgtattcaagtaccaagcaattgtaccaatactagattcccatgttgcccatct 310
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 438 atgatttgtattcaagtaccaagcaattgtaccaatactagattcccatgttgcccatct 379


Query: 311 catccaatttgtattcatccatcaactactgctgcttcaaccattgcaacaactgcatca 370
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 378 catccaatttgtattcatccatcaactactgctgcttcaaccattgcaacaactgcatca 319


Query: 371 actgtcgcaactacaacctctgcaactactgcaggtacaacaactggtggaactacaact 430
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 318 actgtcgcaactacaacctctgcaactactgcaggtacaacaactggtggaactacaact 259


Query: 431 ggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagcgctgcctcctca 490
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 258 ggtggttcaacttctgatagtagtgctgcttcctcagccgatagtagcgctgcctcctca 199


Query: 491 tcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatcaagtagcgccgct 550
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 198 tcaccatcaagcagtgctgcttcaagtgccgcttcaagtgaaccatcaagtagcgccgct 139


Query: 551 tcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcctcaagtagtgct 610
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 138 tcaagtagtgctccatcctctgcttcaagtagcgctccatcctctgcctcaagtagtgct 79


Query: 611 ccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctcagaaagctcagaa 670
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 78  ccatcatcatcagcttcaagttcagctgcatcatcagctgcaagctcagaaagctcagaa 19


Query: 671 agctcatcagccacttc 687
           |||||||||||||||||
Sbjct: 18  agctcatcagccacttc 2

 Score = 63.9 bits (32), Expect(4) = 1e-11
 Identities = 41/44 (93%)
 Strand = Plus / Minus


Query: 548 gcttcaagtagtgctccatcctctgcttcaagtagcgctccatc 591
           ||||||||||| |||||||||||||| |||||||| ||||||||
Sbjct: 117 gcttcaagtagcgctccatcctctgcctcaagtagtgctccatc 74

 Score = 63.9 bits (32), Expect(4) = 1e-11
 Identities = 41/44 (93%)
 Strand = Plus / Minus


Query: 572 gcttcaagtagcgctccatcctctgcctcaagtagtgctccatc 615
           ||||||||||| |||||||||||||| |||||||| ||||||||
Sbjct: 141 gcttcaagtagtgctccatcctctgcttcaagtagcgctccatc 98

 Score = 32.2 bits (16), Expect(4) = 1e-11
 Identities = 16/16 (100%)
 Strand = Plus / Minus


Query: 652 aagctcagaaagctca 667
           ||||||||||||||||
Sbjct: 28  aagctcagaaagctca 13

 Score = 32.2 bits (16), Expect(4) = 1e-11
 Identities = 16/16 (100%)
 Strand = Plus / Minus


Query: 661 aagctcagaaagctca 676
           ||||||||||||||||
Sbjct: 37  aagctcagaaagctca 22

 Score = 26.3 bits (13), Expect(4) = 1e-11
 Identities = 19/21 (90%)
 Strand = Plus / Minus


Query: 615 catcatcagcttcaagttcag 635
           ||||||||||| |||| ||||
Sbjct: 50  catcatcagctgcaagctcag 30

 Score = 26.3 bits (13), Expect(4) = 1e-11
 Identities = 19/21 (90%)
 Strand = Plus / Minus


Query: 639 catcatcagctgcaagctcag 659
           ||||||||||| |||| ||||
Sbjct: 74  catcatcagcttcaagttcag 54

 Score = 26.3 bits (13), Expect(4) = 1e-11
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 13  ccaccaccaccac 25
           |||||||||||||
Sbjct: 673 ccaccaccaccac 661

 Score = 26.3 bits (13), Expect(4) = 1e-11
 Identities = 13/13 (100%)
 Strand = Plus / Minus


Query: 16  ccaccaccaccac 28
           |||||||||||||
Sbjct: 676 ccaccaccaccac 664

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 664,745,927
Number of extensions: 41253468
Number of successful extensions: 4209150
Number of sequences better than 10.0: 1348
Length of query: 687
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 663
Effective length of database: 97,308,875,965
Effective search space: 64515784764795
Effective search space used: 64515784764795
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.24
Homology vs Protein 
Query= Contig-U08500-1 (Contig-U08500-1Q) /CSM_Contig/Contig-U08500-1Q.Seq.d
         (687 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       165   1e-39
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   165   1e-39
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    52   2e-05
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    51   4e-05
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    49   2e-04
Z81540_13(Z81540|pid:none) Caenorhabditis elegans Cosmid F46B3, ...    42   0.013
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    40   0.086
Z98877_12(Z98877|pid:none) Caenorhabditis elegans YAC Y69H2, com...    39   0.11
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    39   0.15
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    39   0.15
AY775805_1(AY775805|pid:none) Ixodes scapularis clone A10_1_02 p...    38   0.25
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...    38   0.33
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    38   0.33
AE013599_444(AE013599|pid:none) Drosophila melanogaster chromoso...    37   0.43
S13904(S13904)antistasin - Mexican leech                               37   0.73
B28806(B28806)antistasin B - Mexican leech                             36   0.95
A28806(A28806)antistasin A - Mexican leech                             36   0.95
AM910990_169(AM910990|pid:none) Plasmodium knowlesi strain H chr...    36   0.95
(P15358) RecName: Full=Antistasin;          Short=ATS; AltName: ...    36   0.95
BX890618_1(BX890618|pid:none) Zebrafish DNA sequence from clone ...    36   0.95
BX088690_1(BX088690|pid:none) Zebrafish DNA sequence from clone ...    36   0.95
AJ306906_1(AJ306906|pid:none) Homo sapiens partial mRNA for fibu...    36   1.2
(Q96RW7) RecName: Full=Hemicentin-1; AltName: Full=Fibulin-6;   ...    36   1.2
AF156100_1(AF156100|pid:none) Homo sapiens hemicentin mRNA, comp...    36   1.2
AL133553_4(AL133553|pid:none) Human DNA sequence from clone GS1-...    36   1.2
AL833232_1(AL833232|pid:none) Homo sapiens mRNA; cDNA DKFZp762L1...    36   1.2
BC172190_1(BC172190|pid:none) Synthetic construct Homo sapiens c...    36   1.2
AM746676_7055(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    35   2.1
T27318(T27318)hypothetical protein Y69H2.3a - Caenorhabditis ele...    35   2.1
T22273(T22273)hypothetical protein F46B3.14 - Caenorhabditis ele...    35   2.1
T27319(T27319)hypothetical protein Y69H2.3b - Caenorhabditis ele...    35   2.1
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    34   3.6
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    34   3.6
Y12539_1(Y12539|pid:none) Branchiostoma floridae mRNA for putati...    33   6.2
JS0209(JS0209) antistasin precursor - Mexican leech  &M24423_1(M...    33   6.2
EU247178_1(EU247178|pid:none) Lycosa singoriensis clone LSsin992...    33   8.0
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    33   8.1
AC116100_6(AC116100|pid:none) Dictyostelium discoideum chromosom...    33   8.1
AP000581_5(AP000581|pid:none) Vibrio parahaemolyticus plasmid pO...    33   8.1
AB043678_5(AB043678|pid:none) Vibrio phage VfO3K6 DNA, complete ...    33   8.1

>(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;
          Length = 555

 Score =  165 bits (418), Expect = 1e-39
 Identities = 69/69 (100%), Positives = 69/69 (100%)
 Frame = +1

Query: 28  QICGSVNCGPGYICTIINGHPTCIRGDGYLCNQTRCPHDYQCETISTNIVKCSPKNDECK 207
           QICGSVNCGPGYICTIINGHPTCIRGDGYLCNQTRCPHDYQCETISTNIVKCSPKNDECK
Sbjct: 275 QICGSVNCGPGYICTIINGHPTCIRGDGYLCNQTRCPHDYQCETISTNIVKCSPKNDECK 334

Query: 208 WHRCPPGSS 234
           WHRCPPGSS
Sbjct: 335 WHRCPPGSS 343

 Score = 51.2 bits (121), Expect(2) = 2e-09
 Identities = 21/21 (100%), Positives = 21/21 (100%)
 Frame = +2

Query: 233 ACNHVGMICIQVPSNCTNTRF 295
           ACNHVGMICIQVPSNCTNTRF
Sbjct: 404 ACNHVGMICIQVPSNCTNTRF 424

 Score = 33.9 bits (76), Expect(2) = 2e-09
 Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 2/48 (4%)
 Frame = +1

Query: 34  CGSVNCGPGYICTIINGHPTCIRGDGY--LCNQTRCPHDYQCETISTN 171
           C    C PG  C      P C+  + +  LC  T+CP D+ C+ I  N
Sbjct: 333 CKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMIRGN 380

 Score = 42.0 bits (97), Expect = 0.017
 Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 9/75 (12%)
 Frame = +1

Query: 34  CGSVNCGPGYICTIINGHPTCI------RGDGYLCNQTRCPHDYQCETI---STNIVKCS 186
           CG++ C  G+ C   +G   C+      R     C    CPH Y C  I   +T +
Sbjct: 176 CGNIQCPWGHYCVNEHGKCRCVPHRPPPRPPVDQCRNQHCPHGYSCRVIKGCATCVRDAR 235

Query: 187 PKNDECKWHRCPPGS 231
           P ++ C+   CP GS
Sbjct: 236 PPHNLCRGFGCPEGS 250

 Score = 37.4 bits (85), Expect = 0.43
 Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
 Frame = +1

Query: 34  CGSVNCGPGYICTIINGHPTCI--RGDGYLCNQTRCPHDYQCETISTNIVKCSPKNDECK 207
           C  V   P Y  +     P C+   G G+ C++ RCP  + CE    +   C P +D C
Sbjct: 119 CEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCPEGFYCEEQGGSAC-CVPHHDGCG 177

Query: 208 WHRCPPG 228
             +CP G
Sbjct: 178 NIQCPWG 184

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 692,040,047
Number of extensions: 13304880
Number of successful extensions: 45660
Number of sequences better than 10.0: 40
Number of HSP's gapped: 45494
Number of HSP's successfully gapped: 62
Length of query: 229
Length of database: 1,040,966,779
Length adjustment: 124
Effective length of query: 105
Effective length of database: 643,635,439
Effective search space: 67581721095
Effective search space used: 67581721095
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.90  gvh: 0.40  alm: 0.39  top: 0.43  tms: 0.00  mit: 0.63  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar

>> prediction for Contig-U08500-1 is mit
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 1
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0