>Contig-U08421-1 (Contig-U08421-1Q) /CSM_Contig/Contig-U08421-1Q.Seq.d
NNNNNNNNNNTGATGAATGNGNTCNATCANCAAANAATTTTCCNGAACCC
CNAAAAAGNTTATTAGATTGGNTAANGCNTTTATTATTAANGGNTTCNAG
NTTCTCTGATGNCNATAAAACGNCAGCNCAAAATTTAGCCATCGNTGNTG
CNCCNAATTTAN

est1=SFI172Z,1,153

XXXDEXXXSXXNFPEPXKXLLDWXXXLLLXXSXFSDXXKTXAQNLAIXXAPNL

Frame A:
xxxxmnxxxxqxifxnpxkxy*ig*xxyy*xxxxslmxikrqxki*psxxxxix


Frame B:
xxx**mxsixkxfsxtpkkxirlxxafiixgfxxl*xx*nxsxkfshrxcxxf


Frame C:
XXXDEXXXSXXNFPEPXKXLLDWXXXLLLXXSXFSDXXKTXAQNLAIXXAPNL

Query= Contig-U08421-1 (Contig-U08421-1Q)
/CSM_Contig/Contig-U08421-1Q.Seq.d
         (162 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08421-1 (Contig-U08421-1Q) /CSM_Contig/Conti...   163  7e-41
Contig-U13983-1 (Contig-U13983-1Q) /CSM_Contig/Conti...   131  2e-31

>Contig-U08421-1 (Contig-U08421-1Q) /CSM_Contig/Contig-U08421-1Q.Seq.d
          Length = 162

 Score =  163 bits (82), Expect = 7e-41
 Identities = 151/151 (100%)
 Strand = Plus / Plus

                                                                       
Query: 11  tgatgaatgngntcnatcancaaanaattttccngaaccccnaaaaagnttattagattg 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 11  tgatgaatgngntcnatcancaaanaattttccngaaccccnaaaaagnttattagattg 70

                                                                       
Query: 71  gntaangcntttattattaanggnttcnagnttctctgatgncnataaaacgncagcnca 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71  gntaangcntttattattaanggnttcnagnttctctgatgncnataaaacgncagcnca 130

                                          
Query: 131 aaatttagccatcgntgntgcnccnaattta 161
           |||||||||||||||||||||||||||||||
Sbjct: 131 aaatttagccatcgntgntgcnccnaattta 161


>Contig-U13983-1 (Contig-U13983-1Q) /CSM_Contig/Contig-U13983-1Q.Seq.d
          Length = 1480

 Score =  131 bits (66), Expect = 2e-31
 Identities = 124/151 (82%)
 Strand = Plus / Plus

                                                                        
Query: 11   tgatgaatgngntcnatcancaaanaattttccngaaccccnaaaaagnttattagattg 70
            ||||||||| | || |||| |||| ||||| || ||||| | |||||| |||||||||||
Sbjct: 969  tgatgaatgcgttcaatcagcaaagaatttaccagaaccacaaaaaagtttattagattg 1028

                                                                        
Query: 71   gntaangcntttattattaanggnttcnagnttctctgatgncnataaaacgncagcnca 130
            | ||| || ||||||||||| || ||| || |||||||||| | |||||| | |||| ||
Sbjct: 1029 gttaatgcatttattattaatggtttcaagtttctctgatgtcaataaaatgacagctca 1088

                                           
Query: 131  aaatttagccatcgntgntgcnccnaattta 161
            ||||||||| |||| || ||| || ||||||
Sbjct: 1089 aaatttagcaatcgttgttgctccaaattta 1119


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 378
Number of Sequences: 6905
Number of extensions: 378
Number of successful extensions: 10
Number of sequences better than 10.0: 2
length of query: 162
length of database: 5,674,871
effective HSP length: 15
effective length of query: 147
effective length of database: 5,571,296
effective search space: 818980512
effective search space used: 818980512
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)

Query= Contig-U08421-1 (Contig-U08421-1Q) /CSM_Contig/Contig-U08421-1Q.Seq.d
         (162 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ402688) Dictyostelium discoideum cDNA clone:dds17o17, 3' ...   163   2e-36  1
(AU039340) Dictyostelium discoideum slug cDNA, clone SLH586.      131   8e-27  1
(AU036979) Dictyostelium discoideum slug cDNA, clone SSA341.      131   8e-27  1
(C94494) Dictyostelium discoideum slug cDNA, clone SSL196.        131   8e-27  1
(C92835) Dictyostelium discoideum slug cDNA, clone SSF457.        131   8e-27  1
(BJ346563) Dictyostelium discoideum cDNA clone:dda24g12, 3' ...   131   8e-27  1
(BJ346314) Dictyostelium discoideum cDNA clone:dda30e17, 3' ...   131   8e-27  1
(BJ346192) Dictyostelium discoideum cDNA clone:dda30l02, 3' ...   131   8e-27  1
(BJ336005) Dictyostelium discoideum cDNA clone:dda52j07, 5' ...   131   8e-27  1
(BJ402113) Dictyostelium discoideum cDNA clone:dds20d16, 3' ...   125   5e-25  1
(BJ402867) Dictyostelium discoideum cDNA clone:dds18c16, 3' ...   119   3e-23  1
(C90062) Dictyostelium discoideum slug cDNA, clone SSG284.        107   1e-19  1
(AU073733) Dictyostelium discoideum slug cDNA, clone SSI185.       60   2e-05  1

>(BJ402688) Dictyostelium discoideum cDNA clone:dds17o17, 3' end,
           single read.
          Length = 153

 Score =  163 bits (82), Expect = 2e-36
 Identities = 151/151 (100%)
 Strand = Plus / Minus


Query: 11  tgatgaatgngntcnatcancaaanaattttccngaaccccnaaaaagnttattagattg 70
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 153 tgatgaatgngntcnatcancaaanaattttccngaaccccnaaaaagnttattagattg 94


Query: 71  gntaangcntttattattaanggnttcnagnttctctgatgncnataaaacgncagcnca 130
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 93  gntaangcntttattattaanggnttcnagnttctctgatgncnataaaacgncagcnca 34


Query: 131 aaatttagccatcgntgntgcnccnaattta 161
           |||||||||||||||||||||||||||||||
Sbjct: 33  aaatttagccatcgntgntgcnccnaattta 3

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 38,985,497
Number of extensions: 1803201
Number of successful extensions: 89431
Number of sequences better than 10.0: 13
Length of query: 162
Length of database: 95,242,211,685
Length adjustment: 22
Effective length of query: 140
Effective length of database: 93,199,600,587
Effective search space: 13047944082180
Effective search space used: 13047944082180
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

Query= Contig-U08421-1 (Contig-U08421-1Q) /CSM_Contig/Contig-U08421-1Q.Seq.d
         (162 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 77,670,865
Number of extensions: 4521619
Number of successful extensions: 149
Number of sequences better than 10.0: 0
Number of HSP's gapped: 149
Number of HSP's successfully gapped: 0
Length of query: 54
Length of database: 1,040,966,779
Length adjustment: 27
Effective length of query: 27
Effective length of database: 954,451,084
Effective search space: 25770179268
Effective search space used: 25770179268
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 26 (14.6 bits)

 psg: 0.75  gvh: 0.70  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U08421-1 is cyt