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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U08265-1
Contig ID Contig-U08265-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U08265-1 (Contig-U08265-1Q) /CSM_Contig/Contig-U08265-1Q.Seq.d
TTTTTTTTTTTTTTTCCCTACTGTATATAAATATTTCTTTTTTTTTTTAT
TTTTTTTTATTTAATAAATTGGTCAATAATGATTAGAAATTGTACACACG
CAGGTAGTTGGTATTTAGACAATGCAAGGAAATTAGAGAAACAATTATCG
GATTGGTTAAGCGAACTTCTTCGTCAAAATCAAAATGTCAAATCAATTAT
AGCACCACATGCAGGTTATTCTTACAGTGGTAGAGCAGCAGCTTATGCAT
ATATTAATTTAATTCCAGAGAATTATAAGAGAGTATTTATTTTAGGGCCA
TCACATCATGTATATATGAAAACTTGTGGTTTAACTAAATTAGATACATG
GGAAACACCAATTGGTAATTTAAAGGTTGATAAAGATACCACAAATAAAC
TCTTTGATACTGGTAGTTTCATTTGGAATACAAAAAGTGTAGATGAAGAT
GAACATNNTTTNGAATTGCAATTCNCCATNTATTGGCCAAAAGTTGCCNG
AAANGNGATTATTNGAATTAAGTATNGTNCCNATTNTGGGTTGGGAANGT
TTATCCAATTGATTTGGGGANGAGTTNTATGGAAAGGATNTTNGGCCNCC
ATATTTTGATGANCCNGAAANCTTTTTTGTAATTTCATCAGATTNCNGNC
ATTGGGGTGAAGATTTGGTTATACCTAGGATTTGCGAATAAGAGGTCCCA
ATCTATAAATTCCATTGAGGAATTGGATCGAAAAGGAATGGAAATCATTG
AAAGTGGTGCCCCTGTTCAATTCCCAAAATATTTAAAAGAAACAAAGAAT
CCAATTTGTGGTAGATGTCCAATTGCTGTAATGTTATGGATCACAAAAAA
TAGTCAATTTAAATATAATATCTCATCACTCTATTATGAACAATCAAGTA
AAGTTATTAAATCTTCTGATTCTTCTGTTAGTTATGGTGTTTTATCTTTT
ACAAAAGAAAATTAAAAAAAAAAAAAAAACATTATAAATAAAAAAATAAA
TAAATAAATGTGGGGGAGATTATTTTATTTTATTTTATTTTGCAATACAG
AATAAATGTGGGATTTGAGTTGGTGATGCAATTGATGGTTTTGTTAAAGG
TACAATATTTGGATCTTTTTTANATTTCATTAATAATANCAACACCCGGN
TAAAAATTNCAATTAAAATAGNGAGACAAAATACAATTTTAACATTANAT
NANCCAGAATCTAAACTCCATGAATATCCTCAACTAAATAAATAAATAAA
TAAATAAATAAATAAATA
Gap no gap
Contig length 1268
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 2635072
End point 2633797
Strand (PLUS/MINUS) MINUS
Number of clones 4
Number of EST 4
Link to clone list U08265
List of clone(s) 

est1=SSJ265E,1,1051
est2=VSH526F,315,459
est3=VSF736F,657,1015
est4=CFI808Z,958,1269
Translated Amino Acid sequence 
fffffpyci*IFLFFFIFFYLINWSIMIRNCTHAGSWYLDNARKLEKQLSDWLSELLRQN
QNVKSIIAPHAGYSYSGRAAAYAYINLIPENYKRVFILGPSHHVYMKTCGLTKLDTWETP
IGNLKVDKDTTNKLFDTGSFIWNTKSVDEDEHXXELQFXXYWPKVAXXXIIXIKYXXXXG
LGXFIQLIWGXVXWKGXXAXIF**xxxlfcnfirxxxlg*rfgyt*dlrirgpnl*iplr
nwiekewkslkvvplfnsqni*kkqriqfvvdvqll*cygsqkivnlniishhsimnnqv
kllnllilllvmvfyllqkkikkkkktl*ikk*inkcggdyfilfyfaiqnkcgi*vgda
idgfvkgtifgsflxfinnxntrxkixikixrqntiltlxxpesklheypqlnk*ink*i
nk
Translated Amino Acid sequence (All Frames) 
Frame A:
fffffpyci*IFLFFFIFFYLINWSIMIRNCTHAGSWYLDNARKLEKQLSDWLSELLRQN
QNVKSIIAPHAGYSYSGRAAAYAYINLIPENYKRVFILGPSHHVYMKTCGLTKLDTWETP
IGNLKVDKDTTNKLFDTGSFIWNTKSVDEDEHXXELQFXXYWPKVAXXXIIXIKYXXXXG
LGXFIQLIWGXVXWKGXXAXIF**xxxlfcnfirxxxlg*rfgyt*dlrirgpnl*iplr
nwiekewkslkvvplfnsqni*kkqriqfvvdvqll*cygsqkivnlniishhsimnnqv
kllnllilllvmvfyllqkkikkkkktl*ikk*inkcggdyfilfyfaiqnkcgi*vgda
idgfvkgtifgsflxfinnxntrxkixikixrqntiltlxxpesklheypqlnk*ink*i
nk


Frame B:
fffffptvykyfffflfffi**igq**leivhtqvvgi*tmqgn*rnnyrig*anffvki
kmsnql*hhmqviltvveqqlmhili*fqriireylf*ghhimyi*klvv*ln*ihgkhq
lvi*rlikipqinslilvvsfgiqkv*mkmnxxxncnspxigqklpexxlxelsxvpixg
wexlsn*fgxexygkdxxppyfdxpexffvissdxxhwgedlviprice*evpiykfh*g
igskrngnh*kwcpcsipkifkrnkesnlw*msnccnvmdhkk*si*i*ylitll*tik*
sy*if*ffc*lwcfifykrklkkkkkhyk*knk*invgeiilfyfilqyrinvgfelvmq
lmvllkvqyldlfxisliixtpg*kxqlk*xdkiqf*hxxxqnlnsmniln*ink*ink*
in


Frame C:
ffffsllyinisfffyfflfnklvnnd*klytrr*lvfrqckeiretiiglvkrtsssks
kcqinysttcrlflqw*ssslciy*fnsrel*esiyfraitsciyenlwfn*irymgntn
w*fkg**ryhk*tl*yw*fhleykkcr*r*txfxiaixhxlakscxkxdyxn*vxxxxwv
gxvypidlgxsxmerxxgxhilmxxkxfl*fhqixxigvkiwlylgfankrsqsinsiee
ldrkgmeiiesgapvqfpkylketknpicgrcpiavmlwitknsqfkynisslyyeqssk
vikssdssvsygvlsftken*kkkkniinkkink*mwgrlfyfilfcnte*mwdlsw*cn
*wfc*ryniwiffxfh**xqhpxknxn*nxetkynfnixxxri*tp*isstk*ink*ink
*i
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U08265-1 (Contig-U08265-1Q)
/CSM_Contig/Contig-U08265-1Q.Seq.d
         (1268 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08265-1 (Contig-U08265-1Q) /CSM_Contig/Conti...  1633  0.0
Contig-U14510-1 (Contig-U14510-1Q) /CSM_Contig/Conti...    36  0.090
Contig-U14697-1 (Contig-U14697-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U12962-1 (Contig-U12962-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U11797-1 (Contig-U11797-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U11342-1 (Contig-U11342-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U05220-1 (Contig-U05220-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U02458-1 (Contig-U02458-1Q) /CSM_Contig/Conti...    34  0.35
Contig-U14737-1 (Contig-U14737-1Q) /CSM_Contig/Conti...    32  1.4

>Contig-U08265-1 (Contig-U08265-1Q) /CSM_Contig/Contig-U08265-1Q.Seq.d
          Length = 1268

 Score = 1633 bits (824), Expect = 0.0
 Identities = 899/899 (100%)
 Strand = Plus / Plus

                                                                       
Query: 59  atttaataaattggtcaataatgattagaaattgtacacacgcaggtagttggtatttag 118
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 59  atttaataaattggtcaataatgattagaaattgtacacacgcaggtagttggtatttag 118

                                                                       
Query: 119 acaatgcaaggaaattagagaaacaattatcggattggttaagcgaacttcttcgtcaaa 178
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 119 acaatgcaaggaaattagagaaacaattatcggattggttaagcgaacttcttcgtcaaa 178

                                                                       
Query: 179 atcaaaatgtcaaatcaattatagcaccacatgcaggttattcttacagtggtagagcag 238
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 179 atcaaaatgtcaaatcaattatagcaccacatgcaggttattcttacagtggtagagcag 238

                                                                       
Query: 239 cagcttatgcatatattaatttaattccagagaattataagagagtatttattttagggc 298
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 239 cagcttatgcatatattaatttaattccagagaattataagagagtatttattttagggc 298

                                                                       
Query: 299 catcacatcatgtatatatgaaaacttgtggtttaactaaattagatacatgggaaacac 358
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 299 catcacatcatgtatatatgaaaacttgtggtttaactaaattagatacatgggaaacac 358

                                                                       
Query: 359 caattggtaatttaaaggttgataaagataccacaaataaactctttgatactggtagtt 418
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 359 caattggtaatttaaaggttgataaagataccacaaataaactctttgatactggtagtt 418

                                                                       
Query: 419 tcatttggaatacaaaaagtgtagatgaagatgaacatnntttngaattgcaattcncca 478
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 419 tcatttggaatacaaaaagtgtagatgaagatgaacatnntttngaattgcaattcncca 478

                                                                       
Query: 479 tntattggccaaaagttgccngaaangngattattngaattaagtatngtnccnattntg 538
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 479 tntattggccaaaagttgccngaaangngattattngaattaagtatngtnccnattntg 538

                                                                       
Query: 539 ggttgggaangtttatccaattgatttggggangagttntatggaaaggatnttnggccn 598
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 539 ggttgggaangtttatccaattgatttggggangagttntatggaaaggatnttnggccn 598

                                                                       
Query: 599 ccatattttgatganccngaaancttttttgtaatttcatcagattncngncattggggt 658
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 599 ccatattttgatganccngaaancttttttgtaatttcatcagattncngncattggggt 658

                                                                       
Query: 659 gaagatttggttatacctaggatttgcgaataagaggtcccaatctataaattccattga 718
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 659 gaagatttggttatacctaggatttgcgaataagaggtcccaatctataaattccattga 718

                                                                       
Query: 719 ggaattggatcgaaaaggaatggaaatcattgaaagtggtgcccctgttcaattcccaaa 778
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 719 ggaattggatcgaaaaggaatggaaatcattgaaagtggtgcccctgttcaattcccaaa 778

                                                                       
Query: 779 atatttaaaagaaacaaagaatccaatttgtggtagatgtccaattgctgtaatgttatg 838
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 779 atatttaaaagaaacaaagaatccaatttgtggtagatgtccaattgctgtaatgttatg 838

                                                                       
Query: 839 gatcacaaaaaatagtcaatttaaatataatatctcatcactctattatgaacaatcaag 898
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 839 gatcacaaaaaatagtcaatttaaatataatatctcatcactctattatgaacaatcaag 898

                                                                      
Query: 899 taaagttattaaatcttctgattcttctgttagttatggtgttttatcttttacaaaag 957
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 899 taaagttattaaatcttctgattcttctgttagttatggtgttttatcttttacaaaag 957


 Score =  335 bits (169), Expect = 7e-92
 Identities = 193/193 (100%)
 Strand = Plus / Plus

                                                                        
Query: 1042 gcaatacagaataaatgtgggatttgagttggtgatgcaattgatggttttgttaaaggt 1101
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1042 gcaatacagaataaatgtgggatttgagttggtgatgcaattgatggttttgttaaaggt 1101

                                                                        
Query: 1102 acaatatttggatcttttttanatttcattaataatancaacacccggntaaaaattnca 1161
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1102 acaatatttggatcttttttanatttcattaataatancaacacccggntaaaaattnca 1161

                                                                        
Query: 1162 attaaaatagngagacaaaatacaattttaacattanatnanccagaatctaaactccat 1221
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1162 attaaaatagngagacaaaatacaattttaacattanatnanccagaatctaaactccat 1221

                         
Query: 1222 gaatatcctcaac 1234
            |||||||||||||
Sbjct: 1222 gaatatcctcaac 1234


>Contig-U14510-1 (Contig-U14510-1Q) /CSM_Contig/Contig-U14510-1Q.Seq.d
          Length = 1389

 Score = 36.2 bits (18), Expect = 0.090
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                  
Query: 431  caaaaagtgtagatgaagatga 452
            |||||| |||||||||||||||
Sbjct: 1055 caaaaactgtagatgaagatga 1076


>Contig-U14697-1 (Contig-U14697-1Q) /CSM_Contig/Contig-U14697-1Q.Seq.d
          Length = 577

 Score = 34.2 bits (17), Expect = 0.35
 Identities = 24/25 (96%), Gaps = 1/25 (4%)
 Strand = Plus / Plus

                                    
Query: 782 tttaaaagaaa-caaagaatccaat 805
           ||||||||||| |||||||||||||
Sbjct: 474 tttaaaagaaatcaaagaatccaat 498


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 13,475
Number of Sequences: 6905
Number of extensions: 13475
Number of successful extensions: 1443
Number of sequences better than 10.0: 146
length of query: 1268
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1252
effective length of database: 5,564,391
effective search space: 6966617532
effective search space used: 6966617532
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.12
Homology vs DNA 
Query= Contig-U08265-1 (Contig-U08265-1Q) /CSM_Contig/Contig-U08265-1Q.Seq.d
         (1268 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C90882) Dictyostelium discoideum slug cDNA, clone SSJ265.        500   0.0    2
(AF020286) Dictyostelium discoideum 2034 gene, partial cds.       375   0.0    5
(AU265705) Dictyostelium discoideum vegetative cDNA clone:VS...   436   e-127  3
(AU074015) Dictyostelium discoideum slug cDNA, clone SSJ265.      353   1e-92  1
(BJ375301) Dictyostelium discoideum cDNA clone:ddc18p02, 3' ...   335   2e-87  1
(AU267576) Dictyostelium discoideum vegetative cDNA clone:VS...   262   3e-65  1
(FG228291) pvreaac010001_C8_M13rev_phred13_vc root hairs of ...    52   0.053  1
(FG228243) pvreaac010001_A4_M13rev_phred13_vc root hairs of ...    52   0.053  1
(DY895733) CeleSEQ15632 Cunninghamella elegans pBluescript (...    34   1.9    3
(EE115768) G708P5140RH3.T0 Acorn worm normalized gastrula pE...    40   2.7    2
(AC173482) Mus musculus BAC clone RP23-383F20 from chromosom...    46   3.2    1
(AC158595) Mus musculus 10 BAC RP24-216B14 (Roswell Park Can...    46   3.2    1
(AC102312) Mus musculus chromosome 10, clone RP24-428L4, com...    46   3.2    1
(DU946953) 401683 Tomato EcoRI BAC Library Solanum lycopersi...    46   3.2    1
(CZ980669) 190849 Tomato MboI BAC Library Solanum lycopersic...    46   3.2    1
(AV423030) Lotus japonicus cDNA clone:MWM021g06_r, 5' end.         46   3.2    1
(AV419519) Lotus japonicus cDNA clone:MWM170d04_r, 5' end.         46   3.2    1
(AV408135) Lotus japonicus cDNA clone:MWL036a06_r, 5' end.         46   3.2    1
(CO514873) s13dSG56D0300030_328406 Glandular trichomes Medic...    46   3.2    1
(AL376557) Medicago truncatula EST MtBB24F05F1 : T3 end of c...    46   3.2    1
(AL372017) Medicago truncatula EST MtBA48A12F1 : T3 end of c...    46   3.2    1
(AL368038) Medicago truncatula EST MtBA21G11F1 : T3 end of c...    46   3.2    1
(AJ804685) Antirrhinum majus EST, clone 018_5_10_k17.              46   3.2    1
(AJ802406) Antirrhinum majus EST, clone 018_5_03_p13.              46   3.2    1
(AJ801876) Antirrhinum majus EST, clone 018_5_02_h16.              46   3.2    1
(AJ801687) Antirrhinum majus EST, clone 018_5_01_p05.              46   3.2    1
(AJ501206) Medicago truncatula EST, clone mtgmadc120004a08.        46   3.2    1
(CF068193) EST668914 MTUS Medicago truncatula cDNA clone MTU...    46   3.2    1
(CA410988) E270_F -P normal and proteoid roots 7 and 10 DAE ...    46   3.2    1
(BW598490) Lotus japonicus cDNA, clone: LjCa-014-CF08, 5' en...    46   3.2    1
(BQ148058) NF058C09FL1F1070 Developing flower Medicago trunc...    46   3.2    1
(BQ123839) EST609415 GLSD Medicago truncatula cDNA clone pGL...    46   3.2    1
(BI271266) NF012D04FL1F1041 Developing flower Medicago trunc...    46   3.2    1
(BI265497) NF083D12IN1F1102 Insect herbivory Medicago trunca...    46   3.2    1
(BG662306) Ljirnpest42-357-a5 Ljirnp Lambda HybriZap two-hyb...    46   3.2    1
(BF520911) EST458384 DSIL Medicago truncatula cDNA clone pDS...    46   3.2    1
(BF519616) EST457080 DSIL Medicago truncatula cDNA clone pDS...    46   3.2    1
(BF519183) EST456644 DSIL Medicago truncatula cDNA clone pDS...    46   3.2    1
(BE998105) EST429828 GVSN Medicago truncatula cDNA clone pGV...    46   3.2    1
(FK715747) pl_014-G9 Ilyanassa (snail) 0-48h Ilyanassa obsol...    46   3.2    1
(FG899516) UCRVU08_CCNS1594_b1 Cowpea IT97K-461-4 Mixed Tiss...    46   3.2    1
(FG883785) UCRVU07_CCNP15555_b1 Cowpea UCR 779 Mixed Tissue ...    46   3.2    1
(FG875011) UCRVU07_CCNP10461_b1 Cowpea UCR 779 Mixed Tissue ...    46   3.2    1
(FG228511) pvreaac010001_O12_M13rev_phred13_vc root hairs of...    46   3.2    1
(FG228509) pvreaac010001_O10_M13rev_phred13_vc root hairs of...    46   3.2    1
(FG228327) pvreaac010001_E6_M13rev_phred13_vc root hairs of ...    46   3.2    1
(FG228252) pvreaac010001_A9_M13rev_phred13_vc root hairs of ...    46   3.2    1
(FG228251) pvreaac010001_A9_M1321_phred13_vc root hairs of P...    46   3.2    1
(FG228250) pvreaac010001_A8_M13rev_phred13_vc root hairs of ...    46   3.2    1
(FG228249) pvreaac010001_A8_M1321_phred13_vc root hairs of P...    46   3.2    1
(FG228246) pvreaac010001_A6_M13rev_phred13_vc root hairs of ...    46   3.2    1
(FG228242) pvreaac010001_A4_M1321_phred13_vc root hairs of P...    46   3.2    1
(FG091648) s13dLA38C01RT006_526067 Lupinus albus L. (white l...    46   3.2    1
(FF395620) MOOFY10TR MOO Vigna unguiculata cDNA 3', mRNA seq...    46   3.2    1
(FF394022) MOODS73TF MOO Vigna unguiculata cDNA 5', mRNA seq...    46   3.2    1
(FE898589) PvEST4444 Bean pod tissue cDNA Entry Library Phas...    46   3.2    1
(EY457910) Pv-RHS24-04 Pv-RHS24 Phaseolus vulgaris cDNA simi...    46   3.2    1
(AC229735) Medicago truncatula clone mth2-36n12, WORKING DRA...    40   3.3    3
(DW089933) CLPY13989.b1_J17.ab1 CLP(XYZ) lettuce perennis La...    40   3.6    2
(AM439389) Vitis vinifera contig VV78X097443.2, whole genome...    38   3.8    2
(DW089917) CLPY13974.b1_L13.ab1 CLP(XYZ) lettuce perennis La...    40   4.0    2
(AZ965109) 2M0235L06F Mouse 10kb plasmid UUGC2M library Mus ...    32   5.1    3
(EH643167) EST14275 LK04 Laupala kohalensis cDNA clone 10610...    36   7.5    3
(BG227371) kq05h07.y1 TBN95TM-SSR Strongyloides stercoralis ...    32   8.3    2
(CT010513) Mouse DNA sequence *** SEQUENCING CANCELLED *** f...    34   9.1    7
(AE014819) Plasmodium falciparum 3D7 chromosome 14 section 4...    38   9.7    7

>(C90882) Dictyostelium discoideum slug cDNA, clone SSJ265.
          Length = 594

 Score =  500 bits (252), Expect(2) = 0.0
 Identities = 252/252 (100%)
 Strand = Plus / Plus


Query: 701 atctataaattccattgaggaattggatcgaaaaggaatggaaatcattgaaagtggtgc 760
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 244 atctataaattccattgaggaattggatcgaaaaggaatggaaatcattgaaagtggtgc 303


Query: 761 ccctgttcaattcccaaaatatttaaaagaaacaaagaatccaatttgtggtagatgtcc 820
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 304 ccctgttcaattcccaaaatatttaaaagaaacaaagaatccaatttgtggtagatgtcc 363


Query: 821 aattgctgtaatgttatggatcacaaaaaatagtcaatttaaatataatatctcatcact 880
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 364 aattgctgtaatgttatggatcacaaaaaatagtcaatttaaatataatatctcatcact 423


Query: 881 ctattatgaacaatcaagtaaagttattaaatcttctgattcttctgttagttatggtgt 940
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 424 ctattatgaacaatcaagtaaagttattaaatcttctgattcttctgttagttatggtgt 483


Query: 941 tttatcttttac 952
           ||||||||||||
Sbjct: 484 tttatcttttac 495

 Score =  337 bits (170), Expect(2) = 0.0
 Identities = 218/242 (90%)
 Strand = Plus / Plus


Query: 459 tttngaattgcaattcnccatntattggccaaaagttgccngaaangngattattngaat 518
           ||| |||||||||||| |||| |||||||||||||||||| |||| | ||||||| ||||
Sbjct: 3   tttggaattgcaattccccatttattggccaaaagttgccggaaaggggattattcgaat 62


Query: 519 taagtatngtnccnattntgggttgggaangtttatccaattgatttggggangagttnt 578
           ||||||| || || ||| ||||||||||| ||||| |||||||||||||||| ||||| |
Sbjct: 63  taagtatggtccccattttgggttgggaaggtttanccaattgatttggggaggagtttt 122


Query: 579 atggaaaggatnttnggccnccatattttgatganccngaaancttttttgtaatttcat 638
           ||||||||||| || |||| |||||||||||||| || |||| |||||||||||||||||
Sbjct: 123 atggaaaggatttttggcccccatattttgatgaccccgaaaccttttttgtaatttcat 182


Query: 639 cagattncngncattggggtgaagatttggttatacctaggatttgcgaataagaggtcc 698
           |||||| | | |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 183 cagattcccgacattggggtgaagatttggttatacctaggatttgcgaataagaggtcc 242


Query: 699 ca 700
           ||
Sbjct: 243 ca 244

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,166,459,529
Number of extensions: 70213357
Number of successful extensions: 5426369
Number of sequences better than 10.0: 66
Length of query: 1268
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1244
Effective length of database: 97,308,875,965
Effective search space: 121052241700460
Effective search space used: 121052241700460
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.24
Homology vs Protein 
Query= Contig-U08265-1 (Contig-U08265-1Q) /CSM_Contig/Contig-U08265-1Q.Seq.d
         (1268 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF020286_1(AF020286|pid:none) Dictyostelium discoideum 2034 gene...   233   1e-59
BT080615_1(BT080615|pid:none) Caligus clemensi clone ccle-evs-50...    95   8e-29
(Q803S3) RecName: Full=Protein MEMO1; AltName: Full=Mediator of ...    90   4e-28
AY814077_1(AY814077|pid:none) Schistosoma japonicum SJCHGC02434 ...    83   2e-23
AK061379_1(AK061379|pid:none) Oryza sativa Japonica Group cDNA c...   110   1e-22
BT040299_1(BT040299|pid:none) Zea mays full-length cDNA clone ZM...   108   5e-22
CR382138_1072(CR382138|pid:none) Debaryomyces hansenii strain CB...    74   9e-21
CR382121_493(CR382121|pid:none) Kluyveromyces lactis strain NRRL...    81   1e-20
EF145426_1(EF145426|pid:none) Populus trichocarpa clone WS01124_...   103   1e-20
AC007070_13(AC007070|pid:none) Arabidopsis thaliana chromosome 2...   102   4e-20
AE017345_123(AE017345|pid:none) Cryptococcus neoformans var. neo...   101   5e-20
AY231849_1(AY231849|pid:none) Drosophila yakuba clone yak-em_CG8...   101   6e-20
CT025895_1(CT025895|pid:none) Zebrafish DNA sequence from clone ...    90   4e-19
(Q91VH6) RecName: Full=Protein MEMO1; AltName: Full=Mediator of ...    97   2e-18
AK159560_1(AK159560|pid:none) Mus musculus osteoclast-like cell ...    97   2e-18
(Q4QQR9) RecName: Full=Protein MEMO1; AltName: Full=Mediator of ...    97   2e-18
AY223018_1(AY223018|pid:none) Schistosoma japonicum clone ZZD112...    96   2e-18
FN357895_6(FN357895|pid:none) Schistosoma mansoni genome sequenc...    96   3e-18
BC070046_1(BC070046|pid:none) Homo sapiens chromosome 2 open rea...    96   3e-18
(Q6GNT9) RecName: Full=Protein MEMO1; AltName: Full=Mediator of ...    95   5e-18
(Q6DJ03) RecName: Full=Protein MEMO1; AltName: Full=Mediator of ...    95   6e-18
(Q10212) RecName: Full=UPF0103 protein C4H3.04c;  &CU329670_1581...    89   3e-16
DQ120661_1(DQ120661|pid:none) Homo sapiens rcC2orf4 gene, partia...    89   4e-16
CR382129_800(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...    86   2e-15
CP001325_314(CP001325|pid:none) Micromonas sp. RCC299 chromosome...    86   3e-15
AP007151_1310(AP007151|pid:none) Aspergillus oryzae RIB40 genomi...    84   8e-15
AE016820_690(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...    84   1e-14
CP000500_36(CP000500|pid:none) Pichia stipitis CBS 6054 chromoso...    73   2e-14
AM910987_219(AM910987|pid:none) Plasmodium knowlesi strain H chr...    83   2e-14
CR380948_158(CR380948|pid:none) Candida glabrata strain CBS138 c...    82   4e-14
(Q22915) RecName: Full=UPF0103 protein tag-253;  &U64857_8(U6485...    82   5e-14
AM920433_639(AM920433|pid:none) Penicillium chrysogenum Wisconsi...    81   9e-14
AB041019_1(AB041019|pid:none) Homo sapiens C2orf4b mRNA, complet...    79   3e-13
(P47085) RecName: Full=UPF0103 protein YJR008W;  &S55196(S55196;...    79   3e-13
FN357895_2(FN357895|pid:none) Schistosoma mansoni genome sequenc...    77   1e-12
FN357895_3(FN357895|pid:none) Schistosoma mansoni genome sequenc...    77   1e-12
FN357895_4(FN357895|pid:none) Schistosoma mansoni genome sequenc...    77   1e-12
AL844503_165(AL844503|pid:none) Plasmodium falciparum 3D7 chromo...    77   1e-12
AM270145_16(AM270145|pid:none) Aspergillus niger contig An07c028...    70   2e-10
DQ875277_1(DQ875277|pid:none) Helicoverpa armigera CG8031-PA-lik...    65   7e-09
CP000252_284(CP000252|pid:none) Syntrophus aciditrophicus SB, co...    57   1e-06
CT005272_119(CT005272|pid:none) Leishmania major strain Friedlin...    42   4e-06
AM502254_147(AM502254|pid:none) Leishmania infantum chromosome 36.     42   7e-06
CP000478_1286(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    52   3e-05
CP000675_1299(CP000675|pid:none) Legionella pneumophila str. Cor...    52   4e-05
CP000606_929(CP000606|pid:none) Shewanella loihica PV-4, complet...    52   4e-05
AE017354_1147(AE017354|pid:none) Legionella pneumophila subsp. p...    52   4e-05
AL035476_47(AL035476|pid:none) Plasmodium falciparum MAL4P3.           52   6e-05
AP009179_977(AP009179|pid:none) Sulfurovum sp. NBC37-1 genomic D...    51   8e-05
CP000879_1058(CP000879|pid:none) Petrotoga mobilis SJ95, complet...    51   8e-05
CT030148_20(CT030148|pid:none) Kuenenia stuttgartiensis genome f...    49   5e-04
AE017261_958(AE017261|pid:none) Picrophilus torridus DSM 9790, c...    48   6e-04
(A9A2Y3) RecName: Full=UPF0103 protein Nmar_0215;  &CP000866_211...    48   6e-04
CP000478_1975(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    47   0.001
AP007255_3706(AP007255|pid:none) Magnetospirillum magneticum AMB...    47   0.002
CP000102_828(CP000102|pid:none) Methanosphaera stadtmanae DSM 30...    47   0.002
CP000859_2423(CP000859|pid:none) Desulfococcus oleovorans Hxd3, ...    45   0.005
CP001097_171(CP001097|pid:none) Chlorobium limicola DSM 245, com...    45   0.007
CP001338_2262(CP001338|pid:none) Candidatus Methanosphaerula pal...    45   0.007
CP001229_165(CP001229|pid:none) Sulfurihydrogenibium azorense Az...    44   0.009
CP001322_3932(CP001322|pid:none) Desulfatibacillum alkenivorans ...    44   0.009
CP001230_113(CP001230|pid:none) Persephonella marina EX-H1, comp...    44   0.009
CP000931_948(CP000931|pid:none) Shewanella halifaxensis HAW-EB4,...    44   0.012
CP000089_1958(CP000089|pid:none) Dechloromonas aromatica RCB, co...    44   0.016
CP000153_1840(CP000153|pid:none) Sulfurimonas denitrificans DSM ...    44   0.016
CP001147_331(CP001147|pid:none) Thermodesulfovibrio yellowstonii...    44   0.016
(A5UN65) RecName: Full=UPF0103 protein Msm_1438;  &CP000678_1438...    44   0.016
(Q8U0F2) RecName: Full=UPF0103 protein PF1638;  &AE009950_1638(A...    44   0.016
CP000116_1586(CP000116|pid:none) Thiobacillus denitrificans ATCC...    42   0.061
AE017180_1819(AE017180|pid:none) Geobacter sulfurreducens PCA, c...    42   0.061
CP001349_3164(CP001349|pid:none) Methylobacterium nodulans ORS 2...    42   0.061
(Q46CL1) RecName: Full=UPF0103 protein Mbar_A1422;  &CP000099_13...    41   0.079
CP001281_2152(CP001281|pid:none) Thauera sp. MZ1T, complete geno...    41   0.079
CP001099_1863(CP001099|pid:none) Chlorobaculum parvum NCIB 8327,...    41   0.10
CU459003_2963(CU459003|pid:none) Magnetospirillum gryphiswaldens...    41   0.10
CP001108_263(CP001108|pid:none) Prosthecochloris aestuarii DSM 2...    40   0.14
(Q8PW40) RecName: Full=UPF0103 protein MM_1761;  &AE008384_1761(...    40   0.14
CP000716_1699(CP000716|pid:none) Thermosipho melanesiensis BI429...    40   0.18
CP000267_390(CP000267|pid:none) Rhodoferax ferrireducens T118, c...    40   0.18
AP009178_1109(AP009178|pid:none) Nitratiruptor sp. SB155-2 genom...    40   0.18
CP001251_1442(CP001251|pid:none) Dictyoglomus turgidum DSM 6724,...    40   0.18
(O67355) RecName: Full=UPF0103 protein aq_1336;  &AE000657_929(A...    40   0.18
CP001390_2978(CP001390|pid:none) Geobacter sp. FRC-32, complete ...    40   0.23
CP001322_4075(CP001322|pid:none) Desulfatibacillum alkenivorans ...    40   0.23
CP000851_895(CP000851|pid:none) Shewanella pealeana ATCC 700345,...    40   0.23
(Q8TT38) RecName: Full=UPF0103 protein MA_0601;  &AE010299_591(A...    39   0.30
AE017221_1342(AE017221|pid:none) Thermus thermophilus HB27, comp...    39   0.30
AP008226_1711(AP008226|pid:none) Thermus thermophilus HB8 genomi...    39   0.39
CP000780_2204(CP000780|pid:none) Candidatus Methanoregula boonei...    39   0.39
(O67039) RecName: Full=UPF0103 protein aq_890;  &AE000657_613(AE...    38   0.88
CP000230_448(CP000230|pid:none) Rhodospirillum rubrum ATCC 11170...    37   1.1
CP001400_1903(CP001400|pid:none) Sulfolobus islandicus M.14.25, ...    37   2.0
CP001146_1331(CP001146|pid:none) Dictyoglomus thermophilum H-6-1...    37   2.0
CP001403_2135(CP001403|pid:none) Sulfolobus islandicus Y.G.57.14...    37   2.0
CP001404_665(CP001404|pid:none) Sulfolobus islandicus Y.N.15.51,...    37   2.0
CR555306_3744(CR555306|pid:none) Azoarcus sp. EbN1 complete geno...    37   2.0
(O26151) RecName: Full=UPF0103 protein MTH_45;  &AE000666_43(AE0...    36   2.6
(A3MS51) RecName: Full=UPF0103 protein Pcal_0028;  &CP000561_28(...    35   4.4
(P95994) RecName: Full=UPF0103 protein SSO0066;  &AE006641_61(AE...    35   4.4
(A4WH12) RecName: Full=UPF0103 protein Pars_0062;  &CP000660_62(...    35   4.4
(Q8ZYE1) RecName: Full=UPF0103 protein PAE0818;  &AE009441_560(A...    35   5.7
AF063866_47(AF063866|pid:none) Melanoplus sanguinipes entomopoxv...    35   5.7
(Q96YW6) RecName: Full=UPF0103 protein ST2062;  &BA000023_2219(B...    35   7.4
CP001124_2274(CP001124|pid:none) Geobacter bemidjiensis Bem, com...    35   7.4
CP001279_808(CP001279|pid:none) Nautilia profundicola AmH, compl...    34   9.7

>AF020286_1(AF020286|pid:none) Dictyostelium discoideum 2034 gene,
           partial cds.
          Length = 168

 Score =  233 bits (594), Expect = 1e-59
 Identities = 116/140 (82%), Positives = 116/140 (82%)
 Frame = +1

Query: 79  MIRNCTHAGSWYLDNARKLEKQLSDWLSELLRQNQNVKSIIAPHXXXXXXXXXXXXXXIN 258
           MIRNCTHAGSWYLDNARKLEKQLSDWLSE  R NQNVKSIIAPH              IN
Sbjct: 1   MIRNCTHAGSWYLDNARKLEKQLSDWLSEASRLNQNVKSIIAPHAGYSYSGRAAAYAYIN 60

Query: 259 LIPENYKRVFILGPSHHVYMKTCGLTKLDTWETPIGNLKVDKDTTNKLFDTGSFIWNTKS 438
           LIPENYKRVFILGPSHHVYMKTCGLTKLDTWETPIGNLKVDKDTTNKLFDTGSFIWNTKS
Sbjct: 61  LIPENYKRVFILGPSHHVYMKTCGLTKLDTWETPIGNLKVDKDTTNKLFDTGSFIWNTKS 120

Query: 439 VDEDEHXXELQFXXYWPKVA 498
           VDEDEH  ELQ   Y  KVA
Sbjct: 121 VDEDEHSLELQL-PYIAKVA 139

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,667,338,060
Number of extensions: 29834737
Number of successful extensions: 91172
Number of sequences better than 10.0: 105
Number of HSP's gapped: 90970
Number of HSP's successfully gapped: 166
Length of query: 422
Length of database: 1,040,966,779
Length adjustment: 131
Effective length of query: 291
Effective length of database: 621,205,444
Effective search space: 180770784204
Effective search space used: 180770784204
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 1.05  gvh: 0.46  alm: 0.26  top: 0.57  tms: 0.07  mit: 0.29  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U08265-1 is nuc
VS (DIR, S) 2
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 1
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0