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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U08144-1
Contig ID Contig-U08144-1
Contig update 2002. 5. 9
Contig sequence 
>Contig-U08144-1 (Contig-U08144-1Q) /CSM_Contig/Contig-U08144-1Q.Seq.d
ATTTTTATTTTTATCATAATATCAAGCAAATTAAAAAAAAAGCACAATGT
CATCTTCAAACAATATCTATCATATTTTAGATGATCTTGTAAAGATGGTA
ATTCGTGCTTTTTACCCCGATGAATATGCTGTTATTATTGATGGTCTTTT
AAGAGAAAAGAAACGTATTAAAGATGAAGATTTAGCATTAAGACTTCGTA
TTCAACAAAAATATGTAAGAAAGATATTAATGGATCTTAAAGGTGATTCA
ATGGTAAAGAGTTCAGATGTAAAAGTAGAAGCAAAAGGACCAAATGAAAG
AGGTAGTACCCATCTACTTTGGTATATAGATTATAAACATATCATTGATA
TTGTAAAATATAAATTATACATGTTTAGAAAGAAGATGGAATCTGTAAAA
GTTCAAAAAATTGACGTTCAAACTTATAAATGTCAAACATGTCATAAAGT
ATATACAGCATTAGATATTCCAAAATTATTAAATATGGACACTGGTGCAT
TGGCATGCGAAATTTGCGATGGCGAATTAGAAGAGGAATTAAATAATGAA
TCATTAACACAAACTGCTAAACATCAATCTGATTTGTTTAGTCAATTAAG
AAAAATTATTGAACAATTAAAGAAAACTGAAGGTCATAATATACCTTTAT
TTGCAAGAGATTTAGCAGATTTATCAGCAGATCAAGGTCCATCTTATACA
ATTAATACCAATTCATCATTAGGTATGGGTCCAAAACCATCTGCATTTCC
TGTTGCACAAGGTGCTGCAACTTCTCATCATATAGATCCAACAAATGAAA
ATATAGAATTTCATGTTGATATTTTAGATACTGATGGTATAGAAATTAAT
AAAGCAGTAGTTAAAAAAGAAAATAAAAAAACAGGATTAGCATCACTTCC
ACCTTGGTTATTGCCAAGTAATTCATTTAAAAATAGAAATGTTAAGTCGA
ATTCAATCTTGAACAATAATCAACAAACTCAAACCTCAACCAACGAACAA
CCTACTGCTGTTAAAGAACAAATTAAAATTGATCAAGATTTTTATATAAA
TTATATTAAAACTCATTATCAAGAATGGGAGAGTGCTCCTGATAGTGGTG
ATGCTGAGGTAAGGTAGTAATAGGGTAGGGGGTTCAACCATNAAGGTA
Gap no gap
Contig length 1148
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 3031668
End point 3030514
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 3
Link to clone list U08144
List of clone(s) 

est1=AFG589F,1,617
est2=CFJ806E,1,1149
est3=CFB864F,2,598
Translated Amino Acid sequence 
flfls*yqan*KKSTMSSSNNIYHILDDLVKMVIRAFYPDEYAVIIDGLLREKKRIKDED
LALRLRIQQKYVRKILMDLKGDSMVKSSDVKVEAKGPNERGSTHLLWYIDYKHIIDIVKY
KLYMFRKKMESVKVQKIDVQTYKCQTCHKVYTALDIPKLLNMDTGALACEICDGELEEEL
NNESLTQTAKHQSDLFSQLRKIIEQLKKTEGHNIPLFARDLADLSADQGPSYTINTNSSL
GMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDILDTDGIEINKAVVKKENKKTGLASLP
PWLLPSNSFKNRNVKSNSILNNNQQTQTSTNEQPTAVKEQIKIDQDFYINYIKTHYQEWE
SAPDSGDAEVR***grgfnhxg
Translated Amino Acid sequence (All Frames) 
Frame A:
ififiiissklkkkhnvifkqylsyfr*sckdgnscflpr*iccyy*wsfkrkety*r*r
fsiktsystkickkdings*r*fngkefrcksrskrtk*kr*ypstlvyrl*tyh*ycki
*iihv*kedgicksskn*rsnl*msnms*siysiryskiikyghwcigmrnlrwrirrgi
k**iintnc*tsi*fv*sikkny*tiken*rs*ytfickrfsrfisrsrsilyn*yqfii
rygskticiscctrccnfssyrsnk*kyrisc*yfry*wyrn**sss*krk*knrisits
tlviak*fi*k*kc*vefnleq*stnsnlnqrttycc*rtn*n*srflykly*nslsrmg
ecs**w*c*gkvvig*gvqpxr


Frame B:
flfls*yqan*KKSTMSSSNNIYHILDDLVKMVIRAFYPDEYAVIIDGLLREKKRIKDED
LALRLRIQQKYVRKILMDLKGDSMVKSSDVKVEAKGPNERGSTHLLWYIDYKHIIDIVKY
KLYMFRKKMESVKVQKIDVQTYKCQTCHKVYTALDIPKLLNMDTGALACEICDGELEEEL
NNESLTQTAKHQSDLFSQLRKIIEQLKKTEGHNIPLFARDLADLSADQGPSYTINTNSSL
GMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDILDTDGIEINKAVVKKENKKTGLASLP
PWLLPSNSFKNRNVKSNSILNNNQQTQTSTNEQPTAVKEQIKIDQDFYINYIKTHYQEWE
SAPDSGDAEVR***grgfnhxg


Frame C:
fyfyhnikqikkkaqchlqtisiif*mil*rw*fvlftpmnmllllmvf*ekrnvlkmki
*h*dfvfnknm*ery*wilkviqw*rvqm*k*kqkdqmkevvpiyfgi*iinislil*ni
nytclerrwnl*kfkkltfklinvkhvikyiqh*ifqny*iwtlvhwhakfaman*krn*
imnh*hkllninliclvn*ekllnn*rklkviiylylqei*qiyqqikvhliqlipihh*
vwvqnhlhfllhkvlqllii*iqqmki*nfmlif*ilmv*klikq*lkkkikkqd*hhfh
lgycqvihlkiemlsriqs*tiinklkpqptnnllllknklklikifi*iilkliikngr
vllivvmlr*gsnrvggstxkv
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U08144-1 (Contig-U08144-1Q)
/CSM_Contig/Contig-U08144-1Q.Seq.d
         (1148 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08144-1 (Contig-U08144-1Q) /CSM_Contig/Conti...  1655  0.0
Contig-U09374-1 (Contig-U09374-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U13241-1 (Contig-U13241-1Q) /CSM_Contig/Conti...    38  0.021
Contig-U13739-1 (Contig-U13739-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U12928-1 (Contig-U12928-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U11931-1 (Contig-U11931-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U11236-1 (Contig-U11236-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U11084-1 (Contig-U11084-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U10396-1 (Contig-U10396-1Q) /CSM_Contig/Conti...    36  0.081
Contig-U09440-1 (Contig-U09440-1Q) /CSM_Contig/Conti...    36  0.081

>Contig-U08144-1 (Contig-U08144-1Q) /CSM_Contig/Contig-U08144-1Q.Seq.d
          Length = 1148

 Score = 1655 bits (835), Expect = 0.0
 Identities = 853/862 (98%)
 Strand = Plus / Plus

                                                                       
Query: 1   atttttatttttatcataatatcaagcaaattnnnnnnnnngcacaatgtcatcttcaaa 60
           ||||||||||||||||||||||||||||||||         |||||||||||||||||||
Sbjct: 1   atttttatttttatcataatatcaagcaaattaaaaaaaaagcacaatgtcatcttcaaa 60

                                                                       
Query: 61  caatatctatcatattttagatgatcttgtaaagatggtaattcgtgctttttaccccga 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  caatatctatcatattttagatgatcttgtaaagatggtaattcgtgctttttaccccga 120

                                                                       
Query: 121 tgaatatgctgttattattgatggtcttttaagagaaaagaaacgtattaaagatgaaga 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tgaatatgctgttattattgatggtcttttaagagaaaagaaacgtattaaagatgaaga 180

                                                                       
Query: 181 tttagcattaagacttcgtattcaacaaaaatatgtaagaaagatattaatggatcttaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 tttagcattaagacttcgtattcaacaaaaatatgtaagaaagatattaatggatcttaa 240

                                                                       
Query: 241 aggtgattcaatggtaaagagttcagatgtaaaagtagaagcaaaaggaccaaatgaaag 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 aggtgattcaatggtaaagagttcagatgtaaaagtagaagcaaaaggaccaaatgaaag 300

                                                                       
Query: 301 aggtagtacccatctactttggtatatagattataaacatatcattgatattgtaaaata 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 aggtagtacccatctactttggtatatagattataaacatatcattgatattgtaaaata 360

                                                                       
Query: 361 taaattatacatgtttagaaagaagatggaatctgtaaaagttcaaaaaattgacgttca 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 taaattatacatgtttagaaagaagatggaatctgtaaaagttcaaaaaattgacgttca 420

                                                                       
Query: 421 aacttataaatgtcaaacatgtcataaagtatatacagcattagatattccaaaattatt 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 aacttataaatgtcaaacatgtcataaagtatatacagcattagatattccaaaattatt 480

                                                                       
Query: 481 aaatatggacactggtgcattggcatgcgaaatttgcgatggcgaattagaagaggaatt 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 aaatatggacactggtgcattggcatgcgaaatttgcgatggcgaattagaagaggaatt 540

                                                                       
Query: 541 aaataatgaatcattaacacaaactgctaaacatcaatctgatttgtttagtcaattaag 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 aaataatgaatcattaacacaaactgctaaacatcaatctgatttgtttagtcaattaag 600

                                                                       
Query: 601 aaaaattattgaacaattaaagaaaactgaaggtcataatatacctttatttgcaagaga 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 aaaaattattgaacaattaaagaaaactgaaggtcataatatacctttatttgcaagaga 660

                                                                       
Query: 661 tttagcagatttatcagcagatcaaggtccatcttatacaattaataccaattcatcatt 720
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661 tttagcagatttatcagcagatcaaggtccatcttatacaattaataccaattcatcatt 720

                                                                       
Query: 721 aggtatgggtccaaaaccatctgcatttcctgttgcacaaggtgctgcaacttctcatca 780
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 721 aggtatgggtccaaaaccatctgcatttcctgttgcacaaggtgctgcaacttctcatca 780

                                                                       
Query: 781 tatagatccaacaaatgaaaatatagaatttcatgttgatattttagatactgatggtat 840
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781 tatagatccaacaaatgaaaatatagaatttcatgttgatattttagatactgatggtat 840

                                 
Query: 841 agaaattaataaagcagtagtt 862
           ||||||||||||||||||||||
Sbjct: 841 agaaattaataaagcagtagtt 862


 Score =  523 bits (264), Expect = e-148
 Identities = 267/267 (100%)
 Strand = Plus / Plus

                                                                        
Query: 882  caggattagcatcacttccaccttggttattgccaagtaattcatttaaaaatagaaatg 941
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 882  caggattagcatcacttccaccttggttattgccaagtaattcatttaaaaatagaaatg 941

                                                                        
Query: 942  ttaagtcgaattcaatcttgaacaataatcaacaaactcaaacctcaaccaacgaacaac 1001
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 942  ttaagtcgaattcaatcttgaacaataatcaacaaactcaaacctcaaccaacgaacaac 1001

                                                                        
Query: 1002 ctactgctgttaaagaacaaattaaaattgatcaagatttttatataaattatattaaaa 1061
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1002 ctactgctgttaaagaacaaattaaaattgatcaagatttttatataaattatattaaaa 1061

                                                                        
Query: 1062 ctcattatcaagaatgggagagtgctcctgatagtggtgatgctgaggtaaggtagtaat 1121
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1062 ctcattatcaagaatgggagagtgctcctgatagtggtgatgctgaggtaaggtagtaat 1121

                                       
Query: 1122 agggtagggggttcaaccatnaaggta 1148
            |||||||||||||||||||||||||||
Sbjct: 1122 agggtagggggttcaaccatnaaggta 1148


>Contig-U09374-1 (Contig-U09374-1Q) /CSM_Contig/Contig-U09374-1Q.Seq.d
          Length = 1095

 Score = 40.1 bits (20), Expect = 0.005
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 792 caaatgaaaatatagaattt 811
           ||||||||||||||||||||
Sbjct: 523 caaatgaaaatatagaattt 542


>Contig-U13241-1 (Contig-U13241-1Q) /CSM_Contig/Contig-U13241-1Q.Seq.d
          Length = 1435

 Score = 38.2 bits (19), Expect = 0.021
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                 
Query: 1  atttttatttttatcataatatc 23
          |||||||||||||| ||||||||
Sbjct: 35 atttttatttttataataatatc 57


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 22,403
Number of Sequences: 6905
Number of extensions: 22403
Number of successful extensions: 2909
Number of sequences better than 10.0: 223
length of query: 1148
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1132
effective length of database: 5,564,391
effective search space: 6298890612
effective search space used: 6298890612
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.12
Homology vs DNA 
Query= Contig-U08144-1 (Contig-U08144-1Q) /CSM_Contig/Contig-U08144-1Q.Seq.d
         (1148 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ325481) Dictyostelium discoideum cDNA clone:dda14a23, 5' ...  1142   0.0    2
(BJ360168) Dictyostelium discoideum cDNA clone:ddc4p16, 5' e...  1104   0.0    2
(BJ362092) Dictyostelium discoideum cDNA clone:ddc20l02, 5' ...  1074   0.0    2
(BJ375828) Dictyostelium discoideum cDNA clone:ddc20l02, 3' ...   852   0.0    2
(AC116330) Dictyostelium discoideum chromosome 2 map 3191214...   567   0.0    6
(CP000942) Ureaplasma parvum serovar 3 str. ATCC 27815, comp...    36   0.001  19
(AE014845) Plasmodium falciparum 3D7 chromosome 12, section ...    44   0.002  10
(BA000021) Wigglesworthia glossinidia endosymbiont of Glossi...    42   0.003  16
(AC005139) Plasmodium falciparum chromosome 12, *** SEQUENCI...    40   0.015  10
(AE017263) Mesoplasma florum L1 complete genome.                   42   0.019  19
(AC005308) Plasmodium falciparum chromosome 12 clone 3D7, **...    44   0.030  9
(AL844509) Plasmodium falciparum chromosome 13.                    38   0.032  15
(AE017308) Mycoplasma mobile 163K complete genome.                 38   0.033  17
(CU469464) Candidatus Phytoplasma mali strain AT complete ch...    38   0.037  17
(AL929352) Plasmodium falciparum strain 3D7, chromosome 5, s...    36   0.074  11
(CP000939) Clostridium botulinum B1 str. Okra, complete genome.    34   0.078  19
(AE014836) Plasmodium falciparum 3D7 chromosome 11 section 1...    34   0.091  13
(AC137701) Medicago truncatula clone mth2-35h1, complete seq...    42   0.11   4
(CP000263) Buchnera aphidicola str. Cc (Cinara cedri), compl...    32   0.13   14
(BD061520) Genome DNA of symbiotic bacteria of aphid.              36   0.18   16
(AR409405) Sequence 1 from patent US 6632935.                      36   0.18   16
(BA000003) Buchnera aphidicola str. APS (Acyrthosiphon pisum...    36   0.18   16
(AE013218) Buchnera aphidicola str. Sg (Schizaphis graminum)...    38   0.18   16
(AM655467) Entamoeba moshkovskii FIC GSS, clone mosh025c01.q1k.    50   0.19   1
(AL844506) Plasmodium falciparum chromosome  7.                    34   0.29   14
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    34   0.30   15
(AE014839) Plasmodium falciparum 3D7 chromosome 11 section 4...    34   0.32   11
(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...    36   0.35   10
(AE015927) Clostridium tetani E88, complete genome.                40   0.36   18
(M34947) P.chabaudi major merozoite surface antigen mRNA, co...    42   0.38   3
(AC215379) Solanum lycopersicum chromosome 2 clone C02HBa007...    44   0.38   6
(EJ472199) 1095403417024 Global-Ocean-Sampling_GS-28-01-01-1...    38   0.53   2
(CK489549) rswab0_007357.y1 swa Bombyx mori cDNA, mRNA seque...    34   0.54   2
(AC106456) Rattus norvegicus clone CH230-155P20, *** SEQUENC...    42   0.55   3
(AF489474) Disogmus areolator NADH dehydrogenase 3 gene, par...    34   0.69   4
(AY334354) Percus corsicus isolate P49 NADH dehydrogenase su...    48   0.74   1
(AY334353) Percus corsicus isolate P48 NADH dehydrogenase su...    48   0.74   1
(AY334352) Percus corsicus isolate P39 NADH dehydrogenase su...    48   0.74   1
(AY334351) Percus corsicus isolate P29 NADH dehydrogenase su...    48   0.74   1
(AY334350) Percus corsicus isolate P46 NADH dehydrogenase su...    48   0.74   1
(AY334349) Percus corsicus isolate P32 NADH dehydrogenase su...    48   0.74   1
(AY334348) Percus corsicus isolate P26 NADH dehydrogenase su...    48   0.74   1
(AY334347) Percus corsicus isolate P45 NADH dehydrogenase su...    48   0.74   1
(AY334346) Percus corsicus isolate P38 NADH dehydrogenase su...    48   0.74   1
(AF537186) Percus corsicus isolate P11 NADH dehydrogenase su...    48   0.74   1
(AL117380) Human DNA sequence from clone RP4-749H19 on chrom...    48   0.74   1
(CU856626) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.74   1
(AL133212) Human DNA sequence *** SEQUENCING CANCELLED *** f...    48   0.74   1
(BQ476837) meladema2g04.b Meladema coriacea cDNA Meladema co...    48   0.74   1
(AE016826) Buchnera aphidicola str. Bp (Baizongia pistaciae)...    38   0.77   12
(AC116960) Dictyostelium discoideum chromosome 2 map complem...    44   0.85   9
(AC178909) Strongylocentrotus purpuratus clone R3-3114P4, WO...    42   0.94   5
(AC226001) Solanum lycopersicum chromosome 2 clone C02HBa011...    34   1.0    9
(AL929356) Plasmodium falciparum strain 3D7, chromosome 9; s...    32   1.1    13
(EK376688) 1095469443920 Global-Ocean-Sampling_GS-31-01-01-1...    34   1.1    3
(AY531391) Schizaphis graminum mitochondrion, complete genome.     32   1.2    6
(AE005174) Escherichia coli O157:H7 EDL933, complete genome.       34   1.2    2
(BA000007) Escherichia coli O157:H7 str. Sakai DNA, complete...    34   1.2    2
(CP001078) Clostridium botulinum E3 str. Alaska E43, complet...    38   1.2    21
(AC175190) Bos taurus clone CH240-174L14, WORKING DRAFT SEQU...    38   1.3    9
(CK816725) UMC-bend_0A01-014-g06 Day 5 Uterus bend Bos tauru...    38   1.3    2
(CK729405) UMC-bend_0B02-006-h08 Day 5 Uterus bend Bos tauru...    38   1.4    2
(CK973091) 4103680 BARC 9BOV Bos taurus cDNA clone 9BOV26_I1...    34   1.4    3
(AC215479) Solanum lycopersicum chromosome 2 clone C02SLm004...    44   1.5    7
(CU633420) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    36   1.5    2
(AG370919) Mus musculus molossinus DNA, clone:MSMg01-176C03....    36   1.5    3
(EJ876175) 1093018370896 Global-Ocean-Sampling_GS-30-02-01-1...    36   1.6    3
(AL845312) Zebrafish DNA sequence from clone DKEY-287F10 in ...    38   1.7    4
(CR944665) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    38   1.8    4
(CU855991) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    38   1.8    5
(AE014849) Plasmodium falciparum 3D7 chromosome 12, section ...    34   1.9    9
(AF121898) Homo sapiens chromosome 8 clone XX-55B12 map q21....    34   1.9    8
(AM483078) Vitis vinifera contig VV78X224272.11, whole genom...    32   1.9    4
(BD184861) Nucleic acid molecule and polypeptide specific to...    34   1.9    2
(AR637596) Sequence 143 from patent US 6855814.                    34   1.9    2
(AR204247) Sequence 143 from patent US 6365723.                    34   1.9    2
(AC109279) Mus musculus chromosome 1, clone RP23-333O12, com...    34   2.0    7
(EJ830872) 1093017580724 Global-Ocean-Sampling_GS-30-02-01-1...    36   2.0    3
(CQ873159) Sequence 772 from Patent WO2004078949.                  38   2.1    4
(EV782822) LB004148.C21_A23 GC_BGC-04 Bos taurus cDNA clone ...    38   2.1    2
(EV701657) LB01957.C21_O22 GC_BGC-19 Bos taurus cDNA clone I...    38   2.3    2
(BX004877) Zebrafish DNA sequence from clone DKEY-7L19 in li...    38   2.3    11
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    34   2.3    14
(DR146743) 49123921 Drosophila pseudoobscura embryonic cDNA ...    38   2.3    3
(AM412317) Clostridium botulinum A str. ATCC 3502 complete g...    34   2.4    19
(AE014818) Plasmodium falciparum 3D7 chromosome 14 section 3...    34   2.4    10
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...    34   2.4    9
(AM456273) Vitis vinifera contig VV79X000559.3, whole genome...    34   2.5    4
(AC163360) Bos taurus clone CH240-138E11, *** SEQUENCING IN ...    38   2.5    7
(AX344566) Sequence 17 from Patent WO0200932.                      34   2.5    10
(AM604684) Entamoeba invadens IP1 GSS, clone inv041c04.p1k.        42   2.6    2
(AE014846) Plasmodium falciparum 3D7 chromosome 12, section ...    34   2.6    11
(AJ813336) Bos sp. EST, clone C0005201k8.                          38   2.6    2
(AM618514) Entamoeba invadens IP1 GSS, clone inv112e11.p1k.        42   2.7    2
(CQ869591) Sequence 12 from Patent WO2004074320.                   46   2.9    1
(AF537183) Percus lineatus isolate P12 NADH dehydrogenase su...    46   2.9    1
(AF190044) Abax parallelepipedus inferior NADH dehydrogenase...    46   2.9    1
(AF190041) Molops piceus NADH dehydrogenase subunit 5 (ND5) ...    46   2.9    1
(AB109863) Nebria lewisi mitochondrial ND5 gene for NADH deh...    46   2.9    1
(AL607023) Human DNA sequence from clone RP11-65E10 on chrom...    46   2.9    1

>(BJ325481) Dictyostelium discoideum cDNA clone:dda14a23, 5' end,
           single read.
          Length = 617

 Score = 1142 bits (576), Expect(2) = 0.0
 Identities = 576/576 (100%)
 Strand = Plus / Plus


Query: 42  gcacaatgtcatcttcaaacaatatctatcatattttagatgatcttgtaaagatggtaa 101
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42  gcacaatgtcatcttcaaacaatatctatcatattttagatgatcttgtaaagatggtaa 101


Query: 102 ttcgtgctttttaccccgatgaatatgctgttattattgatggtcttttaagagaaaaga 161
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 102 ttcgtgctttttaccccgatgaatatgctgttattattgatggtcttttaagagaaaaga 161


Query: 162 aacgtattaaagatgaagatttagcattaagacttcgtattcaacaaaaatatgtaagaa 221
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 162 aacgtattaaagatgaagatttagcattaagacttcgtattcaacaaaaatatgtaagaa 221


Query: 222 agatattaatggatcttaaaggtgattcaatggtaaagagttcagatgtaaaagtagaag 281
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 222 agatattaatggatcttaaaggtgattcaatggtaaagagttcagatgtaaaagtagaag 281


Query: 282 caaaaggaccaaatgaaagaggtagtacccatctactttggtatatagattataaacata 341
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 282 caaaaggaccaaatgaaagaggtagtacccatctactttggtatatagattataaacata 341


Query: 342 tcattgatattgtaaaatataaattatacatgtttagaaagaagatggaatctgtaaaag 401
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 342 tcattgatattgtaaaatataaattatacatgtttagaaagaagatggaatctgtaaaag 401


Query: 402 ttcaaaaaattgacgttcaaacttataaatgtcaaacatgtcataaagtatatacagcat 461
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 402 ttcaaaaaattgacgttcaaacttataaatgtcaaacatgtcataaagtatatacagcat 461


Query: 462 tagatattccaaaattattaaatatggacactggtgcattggcatgcgaaatttgcgatg 521
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 462 tagatattccaaaattattaaatatggacactggtgcattggcatgcgaaatttgcgatg 521


Query: 522 gcgaattagaagaggaattaaataatgaatcattaacacaaactgctaaacatcaatctg 581
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 522 gcgaattagaagaggaattaaataatgaatcattaacacaaactgctaaacatcaatctg 581


Query: 582 atttgtttagtcaattaagaaaaattattgaacaat 617
           ||||||||||||||||||||||||||||||||||||
Sbjct: 582 atttgtttagtcaattaagaaaaattattgaacaat 617

 Score = 63.9 bits (32), Expect(2) = 0.0
 Identities = 32/32 (100%)
 Strand = Plus / Plus


Query: 1  atttttatttttatcataatatcaagcaaatt 32
          ||||||||||||||||||||||||||||||||
Sbjct: 1  atttttatttttatcataatatcaagcaaatt 32

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,480,194,437
Number of extensions: 96591506
Number of successful extensions: 8770627
Number of sequences better than 10.0: 176
Length of query: 1148
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1124
Effective length of database: 97,308,875,965
Effective search space: 109375176584660
Effective search space used: 109375176584660
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.24
Homology vs Protein 
Query= Contig-U08144-1 (Contig-U08144-1Q) /CSM_Contig/Contig-U08144-1Q.Seq.d
         (1148 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116330_27(AC116330|pid:none) Dictyostelium discoideum chromoso...   364   4e-99
AC116330_26(AC116330|pid:none) Dictyostelium discoideum chromoso...   265   2e-69
BC122072_1(BC122072|pid:none) Xenopus tropicalis general transcr...    94   7e-18
FN318307_1(FN318307|pid:none) Schistosoma japonicum isolate Anhu...    91   1e-16
FN318306_1(FN318306|pid:none) Schistosoma japonicum isolate Anhu...    91   1e-16
T27910(T27910) hypothetical protein ZK550.4 - Caenorhabditis ele...    90   2e-16
FN392321_174(FN392321|pid:none) Pichia pastoris GS115 chromosome...    89   2e-16
(P29083) RecName: Full=General transcription factor IIE subunit ...    87   8e-16
AE014296_2125(AE014296|pid:none) Drosophila melanogaster chromos...    87   8e-16
AK223401_1(AK223401|pid:none) Homo sapiens mRNA for general tran...    87   8e-16
(A6QLI8) RecName: Full=General transcription factor IIE subunit ...    87   8e-16
(P36100) RecName: Full=Transcription initiation factor IIE subun...    87   1e-15
BT058806_1(BT058806|pid:none) Salmo salar clone ssal-rgf-504-061...    86   2e-15
CU928174_73(CU928174|pid:none) Zygosaccharomyces rouxii strain C...    86   3e-15
S26646(S26646) transcription factor IIE - African clawed frog  &...    86   3e-15
AE016820_303(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...    85   5e-15
AY809947_1(AY809947|pid:none) Schistosoma japonicum SJCHGC08129 ...    85   5e-15
BC150906_1(BC150906|pid:none) Mus musculus expressed sequence AU...    83   2e-14
CR761226_1(CR761226|pid:none) Xenopus tropicalis finished cDNA, ...    83   2e-14
CR380958_77(CR380958|pid:none) Candida glabrata strain CBS138 ch...    83   2e-14
AY056229_1(AY056229|pid:none) Arabidopsis thaliana unknown prote...    80   1e-13
(Q9P3W1) RecName: Full=Transcription initiation factor IIE subun...    80   2e-13
BT041470_1(BT041470|pid:none) Zea mays full-length cDNA clone ZM...    78   7e-13
BT040123_1(BT040123|pid:none) Zea mays full-length cDNA clone ZM...    77   1e-12
AY463606_1(AY463606|pid:none) Arabidopsis thaliana TFIIE-alpha 2...    74   7e-12
AK067775_1(AK067775|pid:none) Oryza sativa Japonica Group cDNA c...    73   2e-11
AM270267_9(AM270267|pid:none) Aspergillus niger contig An12c0110...    65   4e-09
CR382138_492(CR382138|pid:none) Debaryomyces hansenii strain CBS...    62   4e-08
AL451017_3(AL451017|pid:none) Neurospora crassa DNA linkage grou...    59   4e-07
CU640366_275(CU640366|pid:none) Podospora anserina genomic DNA c...    58   5e-07
AL080254_16(AL080254|pid:none) Arabidopsis thaliana DNA chromoso...    57   2e-06
CP001576_69(CP001576|pid:none) Micromonas sp. RCC299 chromosome ...    56   3e-06
CP000581_556(CP000581|pid:none) Ostreococcus lucimarinus CCE9901...    55   6e-06
FN359059_3(FN359059|pid:none) Schistosoma mansoni genome sequenc...    52   5e-05
CR536605_3(CR536605|pid:none) Zebrafish DNA sequence from clone ...    49   3e-04
CR940347_152(CR940347|pid:none) Theileria annulata strain Ankara...    49   3e-04
CP000882_54(CP000882|pid:none) Hemiselmis andersenii chromosome ...    47   0.002
AX886976_1(AX886976|pid:none) Sequence 2839 from Patent EP1033401.     44   0.011
CP001014_32(CP001014|pid:none) Thermoproteus neutrophilus V24Sta...    38   0.59
(A3MY45) RecName: Full=Transcription factor E;          Short=TF...    38   0.59
AM889285_653(AM889285|pid:none) Gluconacetobacter diazotrophicus...    37   1.00
CP001189_1333(CP001189|pid:none) Gluconacetobacter diazotrophicu...    37   1.00
CP000866_1603(CP000866|pid:none) Nitrosopumilus maritimus SCM1, ...    37   1.7
CP000855_1493(CP000855|pid:none) Thermococcus onnurineus NA1, co...    36   2.2
CP000412_1026(CP000412|pid:none) Lactobacillus delbrueckii subsp...    36   2.9
(P0C0W7) RecName: Full=Trimethylamine methyltransferase mttB;   ...    36   2.9
AE015928_4279(AE015928|pid:none) Bacteroides thetaiotaomicron VP...    35   3.8
(A0RYF4) RecName: Full=Transcription factor E;          Short=TF...    35   3.8
(O93658) RecName: Full=Trimethylamine methyltransferase mttB;   ...    35   5.0
BT067461_1(BT067461|pid:none) Zea mays full-length cDNA clone ZM...    35   5.0
AL844508_136(AL844508|pid:none) Plasmodium falciparum 3D7 chromo...    35   6.5
AB248518_1(AB248518|pid:none) Nicotiana benthamiana mRNA for hyp...    35   6.5
EU016646_27(EU016646|pid:none) Uncultured Group I marine crenarc...    34   8.4
EU159424_1(EU159424|pid:none) Triticum turgidum haplotype B DNA ...    34   8.4
EU016633_24(EU016633|pid:none) Uncultured Group I marine crenarc...    34   8.4
AE010299_911(AE010299|pid:none) Methanosarcina acetivorans str. ...    34   8.4
(Q8TS73) RecName: Full=Trimethylamine methyltransferase mttB2;  ...    34   8.4
EU159422_1(EU159422|pid:none) Aegilops tauschii haplotype D DNA ...    34   8.4
EU159420_1(EU159420|pid:none) Triticum turgidum haplotype B DNA ...    34   8.4
EU159417_1(EU159417|pid:none) Triticum monococcum haplotype A DN...    34   8.4
DQ517337_81(DQ517337|pid:none) Trichoplusia ni ascovirus 2c, com...    34   8.4

>AC116330_27(AC116330|pid:none) Dictyostelium discoideum chromosome 2
            map 3191214-3323468 strain AX4, complete sequence.
          Length = 310

 Score =  364 bits (934), Expect = 4e-99
 Identities = 183/208 (87%), Positives = 184/208 (88%)
 Frame = +2

Query: 485  MDTGALACEICDGXXXXXXXXXSLTQTAKHQSDLFSQLRKIIEQLKKTEGHNIPLFARDL 664
            MDTGALACEICDG         SLTQTAKHQSDLFSQLRKIIEQLKKTEGHNIPLFARDL
Sbjct: 1    MDTGALACEICDGELEEELNNESLTQTAKHQSDLFSQLRKIIEQLKKTEGHNIPLFARDL 60

Query: 665  ADLSADQGPSYTINTNSSLGMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDILDTDGIE 844
            ADLSADQGPSYTINTNSSLGMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDILDTDGIE
Sbjct: 61   ADLSADQGPSYTINTNSSLGMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDILDTDGIE 120

Query: 845  INKAVVKKENKKTGLASLPPWLLPSNSFKNRNVKSNSILXXXXXXXXXXXXXXXAVKEQI 1024
            INKAVVKKENKKTGLASLPPWLLPSNSFKNRNVKSNSIL               AVKEQI
Sbjct: 121  INKAVVKKENKKTGLASLPPWLLPSNSFKNRNVKSNSILNNNQQTQTSTNEQPTAVKEQI 180

Query: 1025 KIDQDFYINYIKTHYQEWESAPDSGDAE 1108
            KIDQDFYINYIKTHYQEWESAPDSGDA+
Sbjct: 181  KIDQDFYINYIKTHYQEWESAPDSGDAD 208

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,551,636,726
Number of extensions: 28617240
Number of successful extensions: 73861
Number of sequences better than 10.0: 61
Number of HSP's gapped: 73767
Number of HSP's successfully gapped: 61
Length of query: 382
Length of database: 1,040,966,779
Length adjustment: 130
Effective length of query: 252
Effective length of database: 624,409,729
Effective search space: 157351251708
Effective search space used: 157351251708
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for Contig-U08144-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 2
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0