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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U08124-1
Contig ID Contig-U08124-1
Contig update 2002. 5. 9
Contig sequence 
>Contig-U08124-1 (Contig-U08124-1Q) /CSM_Contig/Contig-U08124-1Q.Seq.d
ATGTTACATCATNAACTTCCACTGGTCCATCAAATACTCATACTGTATCA
TCAACTTCCATTGATTCACCAACTACTCATGTTACATCATCAACTTCCAC
TGGTCCATCAACTACTCATGCAACACCATCAACTTCTATTGGTCCATTAA
CTTCTCATTTTGCATCATCAACCACTACTGTTCCATCAGCTACTCATGGT
GCACCATAAACTACTTGCGGCAAATCAAGTATTGGTGGTGGTAAAGAAGA
GATAAAACGGATCAACAATCTGGTTTAATATTTTCTATCAGTACCAAACA
ACAATAGAAACCCAAGTGATCAATTTAATCTTGGTACAATTTTGATACAT
AGCAGAAGATAGAAATGAAAAGCAGGTCACCTATTTCCTCGATAATTAAT
TCAACATTGGATGTAAAAAGGGATAAATTTTAGCATATGGTTTTATTTTA
AGGGGATGGGCCCCATTTTCAAAAAATGACGGGGGAACCTATGATCCTGT
GTTCACCAAAGAAGAAGATCATCCAATATCCATCAGCAAAATCAAAAANC
CTAAAATTATCATGAACTTCATGTAATTGTAATAGTAAAAGTGGATAGGT
ATCACACTAAATCCAGAAGTAGTGTTAATAGTAGAAAGCATCAGGTTCGA
TAATAAAACAAATTAATTTCCAACTCAATTGACCCTCACACCACAATGTA
TTATTAGTATGGCTTTGCTCTATACAAAATCAAATGAACCCTCACACCAC
AAGGTCACTATGTCAACGATNTNTTTTACNCAATGTCACAAAGTTCAATT
ATTACGCCNCTTACCAAATGTACAGCTTACACTGATTTAGTAGTTTACAA
TAGCATCGTTCAAACTAATANAGTATTCGCATCATTTGATATACACAACT
TTGACAAAGGCTTCAACTCGTTTTGCATCAACAACCACTCATACTGNATC
ATCCTTTACACGATGCCATCAAGTTTTACCNCCNCTACCTACTAACTTAA
ACTACAGGNCCNCNCTNTTTATCAACTAAGAAGTATAGAATCAAATAAGA
AGAAACAGGTTCNCCNCCGTGACATTTNTTTCNCAAAAATTAAATAATAA
TAATATCAATATCTGTAGTAGATTCAATGTTGATANAAATGAAAAAGCAG
GNATCTGTTGGTTAGATTAGCATTCNCAGATTTCCTCGATAATATTAATT
CGAAATTAGATATAAAAATAGATAACNTTTAGCATTTAGTTTTTTATTTT
AAGGGGATGNGCCCCNTTTTCAAAAAANGACGGGGGAAC
Gap no gap
Contig length 1289
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 280067
End point 278778
Strand (PLUS/MINUS) MINUS
Number of clones 3
Number of EST 4
Link to clone list U08124
List of clone(s) 

est1=AFD810E,1,1290
est2=VSD830Z,249,912
est3=VSD830F,251,893
est4=VSE331Z,297,929
Translated Amino Acid sequence 
vtsxtstgpsnthtvsstsidsptthvtsststgpstthatpstsigpltshfasstttv
psathgap*ttcgkssigggkeeikrinnlv*yflsvpnnnrnpsdqfnlgtilihsrr*
k*kaghlfpr*liqhwm*kginfsiwfyfkgmgpifkk*rgnl*scvhqrrrssnihqqn
qkx*nyhelhvivivkvdryhtksrssvnsrkhqvr**nklisnsidphttmyy*ygfal
ykik*TLTPQGHYVNDXFYXMSQSSIITPLTKCTAYTDLVVYNSIVQTNXVFASFDIHNF
DKGFNSFCINNHSYXIILYTMPSSFTXXTY*lklqxxxxyqlrsiesnkkkqvxxrdixf
xkik***yqyl**iqc*xk*ksrxllvrlafxdfldninskldikidnx*hlvfyfkgmx
pxfkkxrgn
Translated Amino Acid sequence (All Frames) 
Frame A:
mlhhxlplvhqililyhqlplihqllmlhhqlplvhqllmqhhqlllvh*llilhhqpll
fhqllmvhhkllaanqvlvvvkkr*ngstiwfnifyqyqttietqvinlilvqf*yiaed
rnekqvtyfldn*fnigckkg*ilaygfilrgwapfskndggtydpvftkeedhpisisk
ikxpkiimnfm*l***kwigitlnpevvlivesirfdnktn*fptqltltpqciismall
ytksnepshhkvtmstxxftqchkvqllrxlpnvqltli**ftiasfklixyshhliytt
ltkastrfasttthtxssftrchqvlpplptnlnyrxxlfin*ev*nqirrnrfxxvtfx
sqklnnnninicsrfnvdxnekagicwld*hsqissiilirn*i*k*itfsi*ffilrgx
apfskxdgg


Frame B:
cyixnfhwsikysyciinfh*ftnyscyiinfhwsinyscntinfywsinfsfciinhyc
sisyswctinylrqikywww*rrdktdqqsglifsistkqq*kpk*si*swynfdt*qki
emksrspissiinstldvkrdkf*hmvlf*gdgphfqkmtgepmilcspkkkiiqypsak
skxlkls*tscncnsksg*vsh*iqk*c***kasgsiikqinfqln*pshhnvllvwlcs
iqnqmnphttrslcqrxxlxnvtkfnyyaxyqmyslh*fsslq*hrsn*xsirii*ytql
*qrlqlvlhqqplilxhplhdaikfyxxyllt*ttgpxxlstkkyrik*eetgspp*hxf
xkn*iiiisisvvdsmlixmkkqxsvg*isixrfpr*y*feiryknr*xlafsflf*gdx
pxfqkxtge


Frame C:
vtsxtstgpsnthtvsstsidsptthvtsststgpstthatpstsigpltshfasstttv
psathgap*ttcgkssigggkeeikrinnlv*yflsvpnnnrnpsdqfnlgtilihsrr*
k*kaghlfpr*liqhwm*kginfsiwfyfkgmgpifkk*rgnl*scvhqrrrssnihqqn
qkx*nyhelhvivivkvdryhtksrssvnsrkhqvr**nklisnsidphttmyy*ygfal
ykik*TLTPQGHYVNDXFYXMSQSSIITPLTKCTAYTDLVVYNSIVQTNXVFASFDIHNF
DKGFNSFCINNHSYXIILYTMPSSFTXXTY*lklqxxxxyqlrsiesnkkkqvxxrdixf
xkik***yqyl**iqc*xk*ksrxllvrlafxdfldninskldikidnx*hlvfyfkgmx
pxfkkxrgn
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U08124-1 (Contig-U08124-1Q)
/CSM_Contig/Contig-U08124-1Q.Seq.d
         (1289 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U08124-1 (Contig-U08124-1Q) /CSM_Contig/Conti...  2371  0.0
Contig-U14739-1 (Contig-U14739-1Q) /CSM_Contig/Conti...    70  7e-12
Contig-U13437-1 (Contig-U13437-1Q) /CSM_Contig/Conti...    50  6e-06
Contig-U12244-1 (Contig-U12244-1Q) /CSM_Contig/Conti...    50  6e-06
Contig-U14392-1 (Contig-U14392-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U12941-1 (Contig-U12941-1Q) /CSM_Contig/Conti...    42  0.001
Contig-U10237-1 (Contig-U10237-1Q) /CSM_Contig/Conti...    40  0.006
Contig-U13750-1 (Contig-U13750-1Q) /CSM_Contig/Conti...    34  0.36
Contig-U13621-1 (Contig-U13621-1Q) /CSM_Contig/Conti...    34  0.36
Contig-U12800-1 (Contig-U12800-1Q) /CSM_Contig/Conti...    34  0.36

>Contig-U08124-1 (Contig-U08124-1Q) /CSM_Contig/Contig-U08124-1Q.Seq.d
          Length = 1289

 Score = 2371 bits (1196), Expect = 0.0
 Identities = 1283/1289 (99%)
 Strand = Plus / Plus

                                                                        
Query: 1    atgttacatcatnaacttccactggtccatcaaatactcatactgtatcatcaacttcca 60
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1    atgttacatcatnaacttccactggtccatcaaatactcatactgtatcatcaacttcca 60

                                                                        
Query: 61   ttgattcaccaactactcatgttacatcatcaacttccactggtccatcaactactcatg 120
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61   ttgattcaccaactactcatgttacatcatcaacttccactggtccatcaactactcatg 120

                                                                        
Query: 121  caacaccatcaacttctattggtccattaacttctcattttgcatcatcaaccactactg 180
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121  caacaccatcaacttctattggtccattaacttctcattttgcatcatcaaccactactg 180

                                                                        
Query: 181  ttccatcagctactcatggtgcaccataaactacttgcggcaaatcaagtattggtggtg 240
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181  ttccatcagctactcatggtgcaccataaactacttgcggcaaatcaagtattggtggtg 240

                                                                        
Query: 241  gtaaagaagagataaaacggatcaacaatctggtttaatattttctatcagtaccaaaca 300
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241  gtaaagaagagataaaacggatcaacaatctggtttaatattttctatcagtaccaaaca 300

                                                                        
Query: 301  acaatagaaacccaagtgatcaatttaatcttggtacaattttgatacatagcagaagat 360
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301  acaatagaaacccaagtgatcaatttaatcttggtacaattttgatacatagcagaagat 360

                                                                        
Query: 361  agaaatgaaaagcaggtcacctatttcctcgataattaattcaacattggatgtaaaaag 420
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361  agaaatgaaaagcaggtcacctatttcctcgataattaattcaacattggatgtaaaaag 420

                                                                        
Query: 421  ggataaattttagcatatggttttattttaaggggatgggccccattttcaaaaaatgac 480
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421  ggataaattttagcatatggttttattttaaggggatgggccccattttcaaaaaatgac 480

                                                                        
Query: 481  gggggaacctatgatcctgtgttcaccaaagaagaagatcatccaatatccatcagcaaa 540
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481  gggggaacctatgatcctgtgttcaccaaagaagaagatcatccaatatccatcagcaaa 540

                                                                        
Query: 541  atcaaaaancctaaaattatcatgaacttcatgtaattgtaatagtaaaagtggataggt 600
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541  atcaaaaancctaaaattatcatgaacttcatgtaattgtaatagtaaaagtggataggt 600

                                                                        
Query: 601  atcacactaaatccagaagtagtgttaatagtagaaagcatcaggttcgataataaaaca 660
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601  atcacactaaatccagaagtagtgttaatagtagaaagcatcaggttcgataataaaaca 660

                                                                        
Query: 661  aattaatttccaactcaattgaccctcacaccacaatgtattattagtatggctttgctc 720
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661  aattaatttccaactcaattgaccctcacaccacaatgtattattagtatggctttgctc 720

                                                                        
Query: 721  tatacaaaatcaaatgaaccctcacaccacaaggtcactatgtcaacgannnnnnnnacn 780
            ||||||||||||||||||||||||||||||||||||||||||||||||| | |    |||
Sbjct: 721  tatacaaaatcaaatgaaccctcacaccacaaggtcactatgtcaacgatntnttttacn 780

                                                                        
Query: 781  caatgtcacaaagttcaattattacgccncttaccaaatgtacagcttacactgatttag 840
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781  caatgtcacaaagttcaattattacgccncttaccaaatgtacagcttacactgatttag 840

                                                                        
Query: 841  tagtttacaatagcatcgttcaaactaatanagtattcgcatcatttgatatacacaact 900
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 841  tagtttacaatagcatcgttcaaactaatanagtattcgcatcatttgatatacacaact 900

                                                                        
Query: 901  ttgacaaaggcttcaactcgttttgcatcaacaaccactcatactgnatcatcctttaca 960
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 901  ttgacaaaggcttcaactcgttttgcatcaacaaccactcatactgnatcatcctttaca 960

                                                                        
Query: 961  cgatgccatcaagttttaccnccnctacctactaacttaaactacaggnccncnctnttt 1020
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 961  cgatgccatcaagttttaccnccnctacctactaacttaaactacaggnccncnctnttt 1020

                                                                        
Query: 1021 atcaactaagaagtatagaatcaaataagaagaaacaggttcnccnccgtgacatttntt 1080
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1021 atcaactaagaagtatagaatcaaataagaagaaacaggttcnccnccgtgacatttntt 1080

                                                                        
Query: 1081 tcncaaaaattaaataataataatatcaatatctgtagtagattcaatgttgatanaaat 1140
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1081 tcncaaaaattaaataataataatatcaatatctgtagtagattcaatgttgatanaaat 1140

                                                                        
Query: 1141 gaaaaagcaggnatctgttggttagattagcattcncagatttcctcgataatattaatt 1200
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1141 gaaaaagcaggnatctgttggttagattagcattcncagatttcctcgataatattaatt 1200

                                                                        
Query: 1201 cgaaattagatataaaaatagataacntttagcatttagttttttattttaaggggatgn 1260
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1201 cgaaattagatataaaaatagataacntttagcatttagttttttattttaaggggatgn 1260

                                         
Query: 1261 gccccnttttcaaaaaangacgggggaac 1289
            |||||||||||||||||||||||||||||
Sbjct: 1261 gccccnttttcaaaaaangacgggggaac 1289


>Contig-U14739-1 (Contig-U14739-1Q) /CSM_Contig/Contig-U14739-1Q.Seq.d
          Length = 1871

 Score = 69.9 bits (35), Expect = 7e-12
 Identities = 114/140 (81%), Gaps = 2/140 (1%)
 Strand = Plus / Plus

                                                                       
Query: 781 caatgtcacaaagttcaattattacgccncttaccaaatgtacagcttacactgatttag 840
           ||||||||| | |||||| | |||| || ||||| ||| ||||| |||| |||| |||||
Sbjct: 717 caatgtcaccatgttcaactgttacaccacttactaaaggtacaacttatactgttttag 776

                                                                       
Query: 841 tagtttacaatagcatcgttcaaactaatanagtattcgcatcatttg--atatacacaa 898
            |||||||||||  |  ||||||| ||||   ||||||||||||||||  || || ||||
Sbjct: 777 cagtttacaataatactgttcaaattaatggtgtattcgcatcatttggtatctatacaa 836

                               
Query: 899 ctttgacaaaggcttcaact 918
           ||||  ||||||||||||||
Sbjct: 837 ctttcccaaaggcttcaact 856


 Score = 61.9 bits (31), Expect = 2e-09
 Identities = 57/65 (87%), Gaps = 3/65 (4%)
 Strand = Plus / Plus

                                                                       
Query: 70  caactactcatgttacatcatcaacttccactggtccatcaactactcatgcaacaccat 129
           |||||||||||| | ||||||||||   ||||||||||||||||||||||||  || |||
Sbjct: 879 caactactcatgctgcatcatcaac---cactggtccatcaactactcatgctgcatcat 935

                
Query: 130 caact 134
           |||||
Sbjct: 936 caact 940


 Score = 60.0 bits (30), Expect = 6e-09
 Identities = 82/98 (83%), Gaps = 6/98 (6%)
 Strand = Plus / Plus

                                                                       
Query: 21  actggtccatcaaatactcatactgtatcatcaacttccattgattcaccaactactcat 80
           ||||| ||||||| ||||||| ||| |||||||||   || || | || |||||||||||
Sbjct: 869 actggcccatcaactactcatgctgcatcatcaac---cactggtccatcaactactcat 925

                                                 
Query: 81  gttacatcatcaacttccactggtccatcaactactca 118
           | | |||||||||||   ||||||||||||||||||||
Sbjct: 926 gctgcatcatcaact---actggtccatcaactactca 960


 Score = 52.0 bits (26), Expect = 2e-06
 Identities = 35/38 (92%)
 Strand = Plus / Plus

                                                 
Query: 19  ccactggtccatcaaatactcatactgtatcatcaact 56
           ||||||||||||||| ||||||| ||| ||||||||||
Sbjct: 903 ccactggtccatcaactactcatgctgcatcatcaact 940


 Score = 44.1 bits (22), Expect = 4e-04
 Identities = 80/98 (81%), Gaps = 6/98 (6%)
 Strand = Plus / Plus

                                                                       
Query: 99  actggtccatcaactactcatgcaacaccatcaacttctattggtccattaacttctcat 158
           ||||| |||||||||||||||||  || ||||||   | | |||||||| |||| |||||
Sbjct: 869 actggcccatcaactactcatgctgcatcatcaa---ccactggtccatcaactactcat 925

                                                 
Query: 159 tttgcatcatcaaccactactgttccatcagctactca 196
             ||||||||||   ||||||| ||||||| |||||||
Sbjct: 926 gctgcatcatca---actactggtccatcaactactca 960


 Score = 42.1 bits (21), Expect = 0.001
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                    
Query: 692 cacaatgtattattagtatggcttt 716
           ||||| |||||||||||||||||||
Sbjct: 624 cacaaggtattattagtatggcttt 648


 Score = 40.1 bits (20), Expect = 0.006
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 99   actggtccatcaactactca 118
            ||||||||||||||||||||
Sbjct: 1064 actggtccatcaactactca 1083


 Score = 40.1 bits (20), Expect = 0.006
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 99   actggtccatcaactactca 118
            ||||||||||||||||||||
Sbjct: 1034 actggtccatcaactactca 1053


 Score = 36.2 bits (18), Expect = 0.091
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                              
Query: 99   actggtccatcaactact 116
            ||||||||||||||||||
Sbjct: 1097 actggtccatcaactact 1114


 Score = 36.2 bits (18), Expect = 0.091
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 99  actggtccatcaactact 116
           ||||||||||||||||||
Sbjct: 974 actggtccatcaactact 991


 Score = 32.2 bits (16), Expect = 1.4
 Identities = 19/20 (95%)
 Strand = Plus / Plus

                                
Query: 21   actggtccatcaaatactca 40
            ||||||||||||| ||||||
Sbjct: 1064 actggtccatcaactactca 1083


 Score = 32.2 bits (16), Expect = 1.4
 Identities = 19/20 (95%)
 Strand = Plus / Plus

                                
Query: 21   actggtccatcaaatactca 40
            ||||||||||||| ||||||
Sbjct: 1034 actggtccatcaactactca 1053


>Contig-U13437-1 (Contig-U13437-1Q) /CSM_Contig/Contig-U13437-1Q.Seq.d
          Length = 637

 Score = 50.1 bits (25), Expect = 6e-06
 Identities = 88/109 (80%)
 Strand = Plus / Plus

                                                                       
Query: 99  actggtccatcaactactcatgcaacaccatcaacttctattggtccattaacttctcat 158
           |||| |||||| ||||||||||| | |||||||||  ||| ||| |||  ||||  ||||
Sbjct: 411 actgttccatcgactactcatgctaaaccatcaaccactactggcccaccaactaatcat 470

                                                            
Query: 159 tttgcatcatcaaccactactgttccatcagctactcatggtgcaccat 207
             |||| || ||||||||||||  |||||| ||||||||| | ||||||
Sbjct: 471 gctgcaccaccaaccactactggcccatcaactactcatgcttcaccat 519


 Score = 42.1 bits (21), Expect = 0.001
 Identities = 30/33 (90%)
 Strand = Plus / Plus

                                            
Query: 161 tgcatcatcaaccactactgttccatcagctac 193
           |||| ||||||||||||||||| ||||| ||||
Sbjct: 358 tgcaccatcaaccactactgttgcatcaactac 390


 Score = 38.2 bits (19), Expect = 0.023
 Identities = 25/27 (92%)
 Strand = Plus / Plus

                                      
Query: 107 atcaactactcatgcaacaccatcaac 133
           ||||||||||||||| | |||||||||
Sbjct: 228 atcaactactcatgctaaaccatcaac 254


 Score = 32.2 bits (16), Expect = 1.4
 Identities = 25/28 (89%)
 Strand = Plus / Plus

                                       
Query: 166 catcaaccactactgttccatcagctac 193
           |||||||||  |||||||||||| ||||
Sbjct: 248 catcaaccataactgttccatcaactac 275


 Score = 30.2 bits (15), Expect = 5.6
 Identities = 18/19 (94%)
 Strand = Plus / Plus

                               
Query: 1093 aataataataatatcaata 1111
            |||||||||||||| ||||
Sbjct: 526  aataataataatattaata 544


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 32,555
Number of Sequences: 6905
Number of extensions: 32555
Number of successful extensions: 10097
Number of sequences better than 10.0: 716
length of query: 1289
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1273
effective length of database: 5,564,391
effective search space: 7083469743
effective search space used: 7083469743
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2008.11.12
Homology vs DNA 
Query= Contig-U08124-1 (Contig-U08124-1Q) /CSM_Contig/Contig-U08124-1Q.Seq.d
         (1289 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ341750) Dictyostelium discoideum cDNA clone:dda8d04, 3' e...  1475   0.0    1
(BJ324787) Dictyostelium discoideum cDNA clone:dda8d04, 5' e...   914   0.0    3
(AU264620) Dictyostelium discoideum vegetative cDNA clone:VS...   121   7e-23  1
(BJ411337) Dictyostelium discoideum cDNA clone:ddv3p24, 5' e...    84   1e-22  3
(AU264621) Dictyostelium discoideum vegetative cDNA clone:VS...   111   7e-20  1
(C91299) Dictyostelium discoideum slug cDNA, clone SSK221.         42   2e-08  4
(AF298624) Dictyostelium discoideum chromosome 2 repeat region.    48   2e-04  2
(AF298201) Dictyostelium discoideum putative transposon DDT-...    48   2e-04  2
(C94263) Dictyostelium discoideum slug cDNA, clone SSK639.         50   0.003  2
(EJ016119) 1095433018229 Global-Ocean-Sampling_GS-26-01-01-1...    44   0.045  3
(BM357111) 15III-D10 Triphysaria versicolor root-tip, early ...    44   0.10   2
(DR170985) 25_H17:A Triphysaria versicolor root-tip, early D...    44   0.12   2
(AP007497) Lotus japonicus genomic DNA, chromosome 2, clone:...    42   0.13   5
(BJ429563) Dictyostelium discoideum cDNA clone:ddv3p24, 3' e...    40   0.18   3
(AM643518) Entamoeba dispar GSS, clone dispar84f04.p1k.            38   0.55   3
(BJ436675) Dictyostelium discoideum cDNA clone:ddv31l22, 3' ...    36   0.56   3
(BX223752) Danio rerio genomic clone DKEY-242L15, genomic su...    36   0.82   2
(CR855129) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    36   0.82   5
(EU385502) Ursinia speciosa RNA polymerase beta subunit (rpo...    48   0.83   1
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...    48   0.83   1
(CU914563) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    48   0.83   1
(CU856178) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    48   0.83   1
(AC171376) Microcebus murinus clone CH257-12K14, WORKING DRA...    48   0.83   1
(ED337846) AUAC-aap85a10.b1 Ascaris suum whole genome shotgu...    48   0.83   1
(ED073680) AUAC-aat13c11.b1 Ascaris suum whole genome shotgu...    48   0.83   1
(CW952366) TcB38.1_E10_SP6 Tribolium BAC library Tribolium c...    48   0.83   1
(CW594020) OA_ABa0129E04.r OA_ABa Oryza australiensis genomi...    38   0.87   2
(AM488252) Vitis vinifera contig VV78X049503.9, whole genome...    32   0.95   5
(CZ538072) SRAA-aad17d05.g1 Strongyloides ratti whole genome...    40   0.96   2
(AC116049) Homo sapiens BAC clone RP11-396M11 from 4, comple...    46   1.1    3
(AC151331) Xenopus tropicalis clone ISB-302M5, complete sequ...    42   1.3    2
(AL844506) Plasmodium falciparum chromosome  7.                    36   1.5    2
(AM641588) Entamoeba dispar GSS, clone dispar84b04.p1k.            38   1.9    3
(BJ363663) Dictyostelium discoideum cDNA clone:ddc27f21, 5' ...    36   2.3    2
(CB930649) ri57c12.y1 Meloidogyne chitwoodi J2 pDNR LIB Melo...    44   2.4    2
(EY161556) CBTN8529.g1_A21.ab1:P Triphysaria pusilla root ti...    44   2.6    2
(EY183921) CBTI9303.g1_M21.ab1:P Triphysaria pusilla root ti...    44   3.2    2
(CX174782) G11_69-67_13.ab1 leaf inoculated with Marssonia p...    44   3.3    2
(EY168598) CBTI1773.g1_I12.ab1:P Triphysaria pusilla root ti...    44   3.3    2
(CR759811) Zebrafish DNA sequence from clone CH211-190M18 in...    46   3.3    1
(AC154857) Mus musculus BAC clone RP23-16K21 from chromosome...    46   3.3    1
(AC154799) Mus musculus BAC clone RP23-79K21 from chromosome...    46   3.3    1
(EU918171) Prunus simonii S1-RNase gene, partial cds.              46   3.3    1
(EU376959) Prunus simonii S1-RNase (S1-RNase) gene, partial ...    46   3.3    1
(DQ790374) Prunus salicina cultivar Friar Sh-RNase gene, par...    46   3.3    1
(DQ790373) Prunus salicina cultivar Green Sun Sh-RNase gene,...    46   3.3    1
(AB084148) Prunus salicina PS-Sh gene for Sh-RNase, partial ...    46   3.3    1
(AL033525) Human DNA sequence from clone RP5-1108H3 on chrom...    46   3.3    1
(AC111710) Rattus norvegicus clone CH230-40N19, *** SEQUENCI...    46   3.3    1
(AC111586) Rattus norvegicus clone CH230-97J19, *** SEQUENCI...    46   3.3    1
(AC053474) Homo sapiens chromosome 1 clone RP11-223H9 map 1,...    46   3.3    1
(CR938723) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   3.3    1
(CR855382) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   3.3    1
(AC016193) Homo sapiens clone RP11-24B5, WORKING DRAFT SEQUE...    46   3.3    1
(FH109716) CHO_OF3222xo19r1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   3.3    1
(EK117825) 1092963265164 Global-Ocean-Sampling_GS-31-01-01-1...    46   3.3    1
(ED670964) GM_WBa0103P07.r GM_WBa Glycine max genomic clone ...    46   3.3    1
(CZ235306) AIAA-aac65l18.b1 Ancylostoma caninum whole genome...    46   3.3    1
(CG749440) P043-3-F12.za Ppa EcoRI BAC Library Pristionchus ...    46   3.3    1
(CE606449) tigr-gss-dog-17000327555948 Dog Library Canis lup...    46   3.3    1
(BZ980815) PUFIK79TB ZM_0.6_1.0_KB Zea mays genomic clone ZM...    46   3.3    1
(AG349657) Mus musculus molossinus DNA, clone:MSMg01-146A02....    46   3.3    1
(CT763414) Paramecium tetraurelia 5-PRIME EST from clone LK0...    46   3.3    1
(BJ390841) Dictyostelium discoideum cDNA clone:dds23l24, 5' ...    46   3.3    1
(FC295437) CAGN8167.rev CAGN Nematostella vectensis Nemve mi...    46   3.3    1
(FC272732) CAGN20119.rev CAGN Nematostella vectensis Nemve m...    46   3.3    1
(FC255689) CAGN10067.rev CAGN Nematostella vectensis Nemve m...    46   3.3    1
(DQ813663) Sulfolobus metallicus strain DSM 6482 hypothetica...    46   3.3    1
(EY146992) CBTN10596.g1_G10.ab1:P Triphysaria pusilla root t...    44   3.4    2
(AC179426) Strongylocentrotus purpuratus clone R3-62K16, WOR...    38   3.4    5
(ER340268) 1092344339142 Global-Ocean-Sampling_GS-34-01-01-1...    42   3.5    2
(EK424746) 1095516005154 Global-Ocean-Sampling_GS-31-01-01-1...    36   4.1    2
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    38   4.5    7
(BZ695608) SP__Ba0066F20.f SP__Ba Sorghum propinquum genomic...    42   4.7    2
(CR382398) Plasmodium falciparum chromosome 6, complete sequ...    42   4.9    8
(CU571256) Zebrafish DNA sequence from clone CH73-41K6 in li...    40   5.1    4
(AC178273) Strongylocentrotus purpuratus clone R3-1116D10, W...    44   6.0    5
(AL359682) Human chromosome 14 DNA sequence BAC C-2561F5 of ...    44   6.3    3
(AM778099) Carica papaya Y chromosome sequence, clone PH 01I11.    34   6.3    2
(AC202822) Gossypium hirsutum chromosome UNKNOWN clone ZMMBB...    32   6.4    2
(CG037358) PUJBI83TB ZM_0.6_1.0_KB Zea mays genomic clone ZM...    34   8.2    2
(DY907174) CHAX1899.b1_F20.ab1 CHA(XYZ) common wild sunflowe...    34   8.2    2
(DY907983) CHAX2825.b1_B11.ab1 CHA(XYZ) common wild sunflowe...    34   8.3    2
(EC096718) AAE00000434 Acetabularia acetabulum Non-normalize...    34   8.3    2
(CE130090) tigr-gss-dog-17000326070055 Dog Library Canis lup...    34   8.4    2
(AE013053) Thermoanaerobacter tengcongensis MB4, section 80 ...    44   9.5    2
(AC179888) Strongylocentrotus purpuratus clone R3-1006I05, W...    42   9.5    6
(AX346518) Sequence 1589 from Patent WO0200928.                    34   9.8    3

>(BJ341750) Dictyostelium discoideum cDNA clone:dda8d04, 3' end,
            single read.
          Length = 849

 Score = 1475 bits (744), Expect = 0.0
 Identities = 831/840 (98%)
 Strand = Plus / Minus


Query: 450  aaggggatgggccccattttcaaaaaatgacgggggaacctatgatcctgtgttcaccaa 509
            ||||| ||||||||||||||||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 841  aagggnatgggccccattttcaaaaaatgacgggggaacctatnatcctgtgttcnccaa 782


Query: 510  agaagaagatcatccaatatccatcagcaaaatcaaaaancctaaaattatcatgaactt 569
            | |||||||||||||||||||| |||||||||||||||| | ||||||||||||||||||
Sbjct: 781  anaagaagatcatccaatatccntcagcaaaatcaaaaaccntaaaattatcatgaactt 722


Query: 570  catgtaattgtaatagtaaaagtggataggtatcacactaaatccagaagtagtgttaat 629
            |||||||||||||||||||||||||||||||||| | |||||||||||||||||||||||
Sbjct: 721  catgtaattgtaatagtaaaagtggataggtatcncnctaaatccagaagtagtgttaat 662


Query: 630  agtagaaagcatcaggttcgataataaaacaaattaatttccaactcaattgaccctcac 689
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661  agtagaaagcatcaggttcgataataaaacaaattaatttccaactcaattgaccctcac 602


Query: 690  accacaatgtattattagtatggctttgctctatacaaaatcaaatgaaccctcacacca 749
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601  accacaatgtattattagtatggctttgctctatacaaaatcaaatgaaccctcacacca 542


Query: 750  caaggtcactatgtcaacgatntnttttacncaatgtcacaaagttcaattattacgccn 809
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541  caaggtcactatgtcaacgatntnttttacncaatgtcacaaagttcaattattacgccn 482


Query: 810  cttaccaaatgtacagcttacactgatttagtagtttacaatagcatcgttcaaactaat 869
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481  cttaccaaatgtacagcttacactgatttagtagtttacaatagcatcgttcaaactaat 422


Query: 870  anagtattcgcatcatttgatatacacaactttgacaaaggcttcaactcgttttgcatc 929
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421  anagtattcgcatcatttgatatacacaactttgacaaaggcttcaactcgttttgcatc 362


Query: 930  aacaaccactcatactgnatcatcctttacacgatgccatcaagttttaccnccnctacc 989
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361  aacaaccactcatactgnatcatcctttacacgatgccatcaagttttaccnccnctacc 302


Query: 990  tactaacttaaactacaggnccncnctntttatcaactaagaagtatagaatcaaataag 1049
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301  tactaacttaaactacaggnccncnctntttatcaactaagaagtatagaatcaaataag 242


Query: 1050 aagaaacaggttcnccnccgtgacatttntttcncaaaaattaaataataataatatcaa 1109
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241  aagaaacaggttcnccnccgtgacatttntttcncaaaaattaaataataataatatcaa 182


Query: 1110 tatctgtagtagattcaatgttgatanaaatgaaaaagcaggnatctgttggttagatta 1169
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181  tatctgtagtagattcaatgttgatanaaatgaaaaagcaggnatctgttggttagatta 122


Query: 1170 gcattcncagatttcctcgataatattaattcgaaattagatataaaaatagataacntt 1229
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121  gcattcncagatttcctcgataatattaattcgaaattagatataaaaatagataacntt 62


Query: 1230 tagcatttagttttttattttaaggggatgngccccnttttcaaaaaangacgggggaac 1289
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61   tagcatttagttttttattttaaggggatgngccccnttttcaaaaaangacgggggaac 2

 Score = 79.8 bits (40), Expect(2) = 4e-11
 Identities = 46/49 (93%)
 Strand = Plus / Minus


Query: 441 ttttattttaaggggatgggccccattttcaaaaaatgacgggggaacc 489
           |||||||||||||||||| ||||| ||||||||||| ||||||||||||
Sbjct: 49  ttttattttaaggggatgngccccnttttcaaaaaangacgggggaacc 1

 Score = 28.2 bits (14), Expect(2) = 4e-11
 Identities = 14/14 (100%)
 Strand = Plus / Minus


Query: 383 atttcctcgataat 396
           ||||||||||||||
Sbjct: 111 atttcctcgataat 98

 Score = 54.0 bits (27), Expect = 0.014
 Identities = 35/39 (89%)
 Strand = Plus / Minus


Query: 1251 aaggggatgngccccnttttcaaaaaangacgggggaac 1289
            ||||| ||| ||||| ||||||||||| |||||||||||
Sbjct: 841  aagggnatgggccccattttcaaaaaatgacgggggaac 803

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 1,396,074,549
Number of extensions: 92268824
Number of successful extensions: 9639232
Number of sequences better than 10.0: 90
Length of query: 1289
Length of database: 95,242,211,685
Length adjustment: 24
Effective length of query: 1265
Effective length of database: 97,308,875,965
Effective search space: 123095728095725
Effective search space used: 123095728095725
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 6.24
Homology vs Protein 
Query= Contig-U08124-1 (Contig-U08124-1Q) /CSM_Contig/Contig-U08124-1Q.Seq.d
         (1289 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 1,709,621,895
Number of extensions: 30397067
Number of successful extensions: 51262
Number of sequences better than 10.0: 0
Number of HSP's gapped: 51228
Number of HSP's successfully gapped: 0
Length of query: 429
Length of database: 1,040,966,779
Length adjustment: 131
Effective length of query: 298
Effective length of database: 621,205,444
Effective search space: 185119222312
Effective search space used: 185119222312
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.75  gvh: 0.36  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for Contig-U08124-1 is cyt
VS (DIR, S) 2
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 1
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0