>Contig-U06712-1 (Contig-U06712-1Q) /CSM_Contig/Contig-U06712-1Q.Seq.d
AACAACAACAACAACAACAACAACAACAAAATCAACAACAAAATAATTCT
TCAGAACTCTCTTCACCTTCTTCTTCATTCTCATCATTAAATAATTCGAT
GATTAATAACAGAAATGATGATTGTGGAAATTTGGAATTTTCATTCAATA
AAATGTTTAATCATCACCATCAACAAAATACAGTCATAAATACAAACTAA
AAAAAATATATCACTAATTAAATAATAGTTCAACTTGTACAAAGCCTTTG
TAGATTCAATTCCAAATAAAAAAAAA

est1=VSF507F,1,277
est2=VSF507Z,1,245

QQQQQQQQQNQQQNNSSELSSPSSSFSSLNNSMINNRNDDCGNLEFSFNKMFNHHHQQNT
VINTN*kkyitn*iivqlvqslcrfnsk*kk

Frame A:
nnnnnnnnnkinnkiilqnslhlllhshh*iir*litemmiveiwnfhsikcliitinki
qs*iqtkknislik**fnlykafvdsipnkkk


Frame B:
tttttttttksttk*ffrtlftfffiliik*fdd**qk**lwkfgifiq*nv*sspstky
shkyklkkiyh*lnnsstctkpl*iqfqikk


Frame C:
QQQQQQQQQNQQQNNSSELSSPSSSFSSLNNSMINNRNDDCGNLEFSFNKMFNHHHQQNT
VINTN*kkyitn*iivqlvqslcrfnsk*kk

Query= Contig-U06712-1 (Contig-U06712-1Q)
/CSM_Contig/Contig-U06712-1Q.Seq.d
         (276 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U06712-1 (Contig-U06712-1Q) /CSM_Contig/Conti...   400  e-112
Contig-U09229-1 (Contig-U09229-1Q) /CSM_Contig/Conti...    38  0.005
Contig-U08357-1 (Contig-U08357-1Q) /CSM_Contig/Conti...    38  0.005
Contig-U12507-1 (Contig-U12507-1Q) /CSM_Contig/Conti...    36  0.019
Contig-U07113-1 (Contig-U07113-1Q) /CSM_Contig/Conti...    36  0.019
Contig-U06747-1 (Contig-U06747-1Q) /CSM_Contig/Conti...    36  0.019
Contig-U01641-1 (Contig-U01641-1Q) /CSM_Contig/Conti...    36  0.019
Contig-U13865-1 (Contig-U13865-1Q) /CSM_Contig/Conti...    34  0.074
Contig-U12548-1 (Contig-U12548-1Q) /CSM_Contig/Conti...    34  0.074
Contig-U11921-1 (Contig-U11921-1Q) /CSM_Contig/Conti...    34  0.074

>Contig-U06712-1 (Contig-U06712-1Q) /CSM_Contig/Contig-U06712-1Q.Seq.d
          Length = 276

 Score =  400 bits (202), Expect = e-112
 Identities = 218/226 (96%)
 Strand = Plus / Plus

                                                                       
Query: 42  aataattcttcagaactctcttcaccttcttcttcattctcatcattaaataattcgatg 101
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42  aataattcttcagaactctcttcaccttcttcttcattctcatcattaaataattcgatg 101

                                                                       
Query: 102 attaataacagaaatgatgattgtggaaatttggaattttcattcaataaaatgtttaat 161
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 102 attaataacagaaatgatgattgtggaaatttggaattttcattcaataaaatgtttaat 161

                                                                       
Query: 162 catcaccatcaacaaaatacagtcataaatacaaactnnnnnnnntatatcactaattaa 221
           |||||||||||||||||||||||||||||||||||||        |||||||||||||||
Sbjct: 162 catcaccatcaacaaaatacagtcataaatacaaactaaaaaaaatatatcactaattaa 221

                                                         
Query: 222 ataatagttcaacttgtacaaagcctttgtagattcaattccaaat 267
           ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 222 ataatagttcaacttgtacaaagcctttgtagattcaattccaaat 267


>Contig-U09229-1 (Contig-U09229-1Q) /CSM_Contig/Contig-U09229-1Q.Seq.d
          Length = 1383

 Score = 38.2 bits (19), Expect = 0.005
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 71  ttcttcattctcatcatta 89
           |||||||||||||||||||
Sbjct: 765 ttcttcattctcatcatta 783


>Contig-U08357-1 (Contig-U08357-1Q) /CSM_Contig/Contig-U08357-1Q.Seq.d
          Length = 290

 Score = 38.2 bits (19), Expect = 0.005
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 153 atgtttaatcatcaccatcaaca 175
           |||||| ||||||||||||||||
Sbjct: 180 atgttttatcatcaccatcaaca 202


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 4718
Number of Sequences: 6905
Number of extensions: 4718
Number of successful extensions: 1651
Number of sequences better than 10.0: 209
length of query: 276
length of database: 5,674,871
effective HSP length: 15
effective length of query: 261
effective length of database: 5,571,296
effective search space: 1454108256
effective search space used: 1454108256
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)

Query= Contig-U06712-1 (Contig-U06712-1Q) /CSM_Contig/Contig-U06712-1Q.Seq.d
         (276 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU265337) Dictyostelium discoideum vegetative cDNA clone:VS...   307   e-110  2
(AU265338) Dictyostelium discoideum vegetative cDNA clone:VS...   307   1e-96  2
(AM475906) Vitis vinifera, whole genome shotgun sequence, co...    50   0.040  1
(AC212302) Solanum lycopersicum chromosome 05 clone C05HBa01...    50   0.040  1
(CU463987) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    42   0.12   5
(DY893561) CeleSEQ12934 Cunninghamella elegans pBluescript (...    36   0.14   2
(ED273826) AUAC-aau79d05.b1 Ascaris suum whole genome shotgu...    48   0.16   1
(BZ429732) BONIY55TF BO_1.6_2_KB_tot Brassica oleracea genom...    48   0.16   1
(FF614739) G825P5181FH3.T0 Acorn worm normalized gastrula pE...    48   0.16   1
(FF510960) G709P537RD12.T0 Acorn worm normalized neurula pEx...    48   0.16   1
(FF440772) G142P60272RF8.T0 Acorn worm juvenile pCMVSport6 l...    48   0.16   1
(FF437793) G142P60222RG12.T0 Acorn worm juvenile pCMVSport6 ...    48   0.16   1
(FF419006) G125P60000RB3.T0 Acorn worm blastula/gastrula pCM...    48   0.16   1
(DY450676) PCAA-aaa25e02.g2 UCI-12 Podocoryne carnea cDNA 5'...    36   0.48   2
(EJ260947) 1095349038327 Global-Ocean-Sampling_GS-27-01-01-1...    46   0.63   1
(EL438944) CHTM14138.b1_D08.ab1 CHT(LMS) Jerusalem artichoke...    46   0.63   1
(DY909862) CHAX4830.b1_K08.ab1 CHA(XYZ) common wild sunflowe...    46   0.63   1
(CX580235) TTE00029523 Amplicon Express - Conjugative Form T...    46   0.63   1
(C22922) Dictyostelium discoideum gamete cDNA, clone FC-AM03.      46   0.63   1
(FD732870) WS03818.C21_O20 SS-B-23 Picea sitchensis cDNA clo...    46   0.63   1
(EV845662) FTSCC92TF Tetrahymena thermophila SB210 cDNA libr...    46   0.63   1
(BX942845) Zebrafish DNA sequence *** SEQUENCING CANCELLED *...    40   1.2    3
(AC023581) Homo sapiens chromosome 5 clone RP11-214O8 map 5,...    38   1.2    2
(L22219) Plasmodium falciparum transmission-blocking target ...    38   1.5    2
(AF269242) Plasmodium falciparum transmission-blocking targe...    38   1.5    2
(I95887) Sequence 1 from patent US 5733772.                        38   1.5    2
(AR178096) Sequence 1 from patent US 6316000.                      38   1.5    2
(L08135) Plasmodium falciparum transmission blocking target ...    38   1.5    2
(L04162) Plasmodium falciparum (clone sY) s230 mRNA, complet...    38   1.5    2
(BX510301) Zebrafish DNA sequence from clone CH211-215A10 in...    40   1.7    2
(CR382139) Debaryomyces hansenii chromosome G of strain CBS7...    36   2.1    8
(AE001393) Plasmodium falciparum 3D7 chromosome 2 section 30...    38   2.3    2
(BX950218) Zebrafish DNA sequence from clone DKEY-276J7 in l...    44   2.5    1
(AC132358) Mus musculus BAC clone RP24-133O24 from chromosom...    44   2.5    1
(U95973) Arabidopsis thaliana chromosome 1 BAC T19D16 genomi...    44   2.5    1
(AY093130) Arabidopsis thaliana Ser/Thr kinase (At1g10940) m...    44   2.5    1
(CS541767) Sequence 3 from Patent WO2006008271.                    44   2.5    1
(AX461583) Sequence 512 from Patent WO0198480.                     44   2.5    1
(AX461229) Sequence 158 from Patent WO0198480.                     44   2.5    1
(AC159744) Mus musculus clone RP23-329A24, WORKING DRAFT SEQ...    44   2.5    1
(AC156402) Bos taurus clone CH240-59P15, WORKING DRAFT SEQUE...    44   2.5    1
(CU640375) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   2.5    1
(CU062475) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   2.5    1
(AC221974) Bos taurus clone CH240-377C22, WORKING DRAFT SEQU...    44   2.5    1
(AC020825) Mus musculus chromosome 18 clone RG-MBAC_340N18, ...    44   2.5    1
(AZ534164) ENTBR19TF Entamoeba histolytica Sheared DNA Entam...    44   2.5    1
(EK130121) 1093006403901 Global-Ocean-Sampling_GS-31-01-01-1...    44   2.5    1
(CL607514) CH240_173D23.TJ CHORI-240 Bos taurus genomic clon...    44   2.5    1
(CC275871) CH261-37L14_Sp6.1 CH261 Gallus gallus genomic clo...    44   2.5    1
(AF005793) AF005793 Arabidopsis thaliana 094-1 Arabidopsis t...    44   2.5    1
(EL400945) CFFS12223.b1_N08.ab1 CFF(LMS) safflower Carthamus...    44   2.5    1
(EL358276) CCEM3106.b1_C09.ab1 CCE(LMS) endive Cichorium end...    44   2.5    1
(EL357660) CCEM2477.b1_J19.ab1 CCE(LMS) endive Cichorium end...    44   2.5    1
(EL354952) CCEM11610.b1_C23.ab1 CCE(LMS) endive Cichorium en...    44   2.5    1
(EH686211) CCIM11005.b1_I16.ab1 CCI(LMS) chicory Cichorium i...    44   2.5    1
(EH685974) CCIM10766.b1_K03.ab1 CCI(LMS) chicory Cichorium i...    44   2.5    1
(EH685293) CCIM10084.b1_H01.ab1 CCI(LMS) chicory Cichorium i...    44   2.5    1
(DW575871) EST_ssal_rgb2_40290 rgb2 Salmo salar cDNA clone s...    44   2.5    1
(DR286214) 6643291 CERES-AL46 Arabidopsis thaliana cDNA clon...    44   2.5    1
(DR286209) 11074785 CERES-AN65 Arabidopsis thaliana cDNA clo...    44   2.5    1
(CU070239) AGENAE Rainbow trout multi-tissues library (tcce)...    44   2.5    1
(BX300823) AGENAE Rainbow trout multi-tissues - normalized +...    44   2.5    1
(EW818651) G893P544FA24.T0 Ixodes scapularis Pooled Non-infe...    44   2.5    1
(EV239141) VV_PEc11H03.r.ab1 Vitis vinifera cv. perlette Lib...    44   2.5    1
(EV239140) VV_PEc11H03.f.ab1 Vitis vinifera cv. perlette Lib...    44   2.5    1
(AV828537) Arabidopsis thaliana cDNA clone:RAFL09-29-G18, 5'...    44   2.5    1
(DJ025884) Genome-wide DNA marker of Saccharomyces cerevisiae.     36   2.6    5
(AC044901) Homo sapiens chromosome 3 clone RP11-771O11 map 3...    36   3.0    5
(CG751678) P046-1-G01.za Ppa EcoRI BAC Library Pristionchus ...    36   3.1    2
(AC116919) Dictyostelium discoideum chromosome 2 map complem...    36   3.1    4
(CG745755) P038-4-B03.za Ppa EcoRI BAC Library Pristionchus ...    36   3.2    2
(AP010009) Lotus japonicus genomic DNA, chromosome 4, clone:...    36   3.4    3
(AP005269) Pan troglodytes DNA, chromosome 7 clone:PTB-102E09.     36   3.7    5
(AC021948) Homo sapiens clone RP11-276D8, WORKING DRAFT SEQU...    38   5.3    3
(EB443948) KR2B.102B06F.051227T7 KR2B Nicotiana tabacum cDNA...    32   6.3    2
(DT749130) EST1182979 Aquilegia cDNA library Aquilegia formo...    32   7.4    2
(AC223341) Bos taurus clone CH240-422A12, WORKING DRAFT SEQU...    40   7.8    2
(DR939007) EST1130546 Aquilegia cDNA library Aquilegia formo...    32   8.1    2
(AM462711) Vitis vinifera contig VV78X026811.3, whole genome...    40   9.0    2
(CU468966) Zebrafish DNA sequence from clone CH73-85C11 in l...    42   9.8    1
(CU207343) Zebrafish DNA sequence from clone CH211-285E16.         42   9.8    1
(BX927410) Zebrafish DNA sequence from clone CH211-51O12 in ...    42   9.8    1
(BX927396) Zebrafish DNA sequence from clone DKEY-216H18 in ...    42   9.8    1
(BX784038) Zebrafish DNA sequence from clone CH211-217K23 in...    42   9.8    1
(BX569792) Zebrafish DNA sequence from clone CH211-197I17 in...    42   9.8    1
(BX510654) Zebrafish DNA sequence from clone DKEY-271N12 in ...    42   9.8    1
(BX470263) Zebrafish DNA sequence from clone DKEY-15P9 in li...    42   9.8    1
(BX470256) Zebrafish DNA sequence from clone CH211-279A3 in ...    42   9.8    1
(BX322550) Zebrafish DNA sequence from clone DKEY-202N14 in ...    42   9.8    1
(BX248118) Zebrafish DNA sequence from clone DKEY-100H21 in ...    42   9.8    1
(AL953867) Zebrafish DNA sequence from clone CH211-258L4 in ...    42   9.8    1
(AL929243) Zebrafish DNA sequence from clone DKEYP-15G8.           42   9.8    1
(BV603348) S217P6658RD11.T0 Noemie Pan troglodytes troglodyt...    42   9.8    1
(AL833800) Mouse DNA sequence from clone RP23-147P20 on chro...    42   9.8    1
(AC155639) Mus musculus 10 BAC RP24-531I22 (Roswell Park Can...    42   9.8    1
(AC153924) Mus musculus 10 BAC RP23-74P12 (Roswell Park Canc...    42   9.8    1
(AC141647) Mus musculus BAC clone RP23-64E12 from chromosome...    42   9.8    1
(BT004973) Arabidopsis thaliana clone U50495 unknown protein...    42   9.8    1
(AK117689) Arabidopsis thaliana At2g38300 mRNA for unknown p...    42   9.8    1
(AJ884919) Plantago major suc1 gene for sucrose transporter,...    42   9.8    1

>(AU265337) Dictyostelium discoideum vegetative cDNA clone:VSF507,
           5' end single read.
          Length = 277

 Score =  307 bits (155), Expect(2) = e-110
 Identities = 155/155 (100%)
 Strand = Plus / Plus


Query: 44  taattcttcagaactctcttcaccttcttcttcattctcatcattaaataattcgatgat 103
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 44  taattcttcagaactctcttcaccttcttcttcattctcatcattaaataattcgatgat 103


Query: 104 taataacagaaatgatgattgtggaaatttggaattttcattcaataaaatgtttaatca 163
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 104 taataacagaaatgatgattgtggaaatttggaattttcattcaataaaatgtttaatca 163


Query: 164 tcaccatcaacaaaatacagtcataaatacaaact 198
           |||||||||||||||||||||||||||||||||||
Sbjct: 164 tcaccatcaacaaaatacagtcataaatacaaact 198

 Score =  121 bits (61), Expect(2) = e-110
 Identities = 61/61 (100%)
 Strand = Plus / Plus


Query: 207 tatatcactaattaaataatagttcaacttgtacaaagcctttgtagattcaattccaaa 266
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 207 tatatcactaattaaataatagttcaacttgtacaaagcctttgtagattcaattccaaa 266


Query: 267 t 267
           |
Sbjct: 267 t 267

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 318,789,497
Number of extensions: 21627863
Number of successful extensions: 1984909
Number of sequences better than 10.0: 175
Length of query: 276
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 253
Effective length of database: 93,106,754,628
Effective search space: 23556008920884
Effective search space used: 23556008920884
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

Query= Contig-U06712-1 (Contig-U06712-1Q) /CSM_Contig/Contig-U06712-1Q.Seq.d
         (276 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR626927_336(CR626927|pid:none) Bacteroides fragilis NCTC 9343, ...    32   7.0
AP006841_398(AP006841|pid:none) Bacteroides fragilis YCH46 DNA, ...    32   9.2

>CR626927_336(CR626927|pid:none) Bacteroides fragilis NCTC 9343,
           complete genome.
          Length = 94

 Score = 32.0 bits (71), Expect = 7.0
 Identities = 16/41 (39%), Positives = 25/41 (60%)
 Frame = -2

Query: 272 FLFGIESTKALYKLNYYLISDIFFLVCIYDCILLMVMIKHF 150
           +L  +EST  LY++   L SDIF + C +D   L+V++  F
Sbjct: 25  YLKHMESTDGLYEMRITLGSDIFCVFCFFDKGRLVVLLSGF 65

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 241,965,955
Number of extensions: 2794250
Number of successful extensions: 7130
Number of sequences better than 10.0: 2
Number of HSP's gapped: 7085
Number of HSP's successfully gapped: 2
Length of query: 92
Length of database: 1,040,966,779
Length adjustment: 61
Effective length of query: 31
Effective length of database: 845,505,394
Effective search space: 26210667214
Effective search space used: 26210667214
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 28 (15.4 bits)

 psg: 0.49  gvh: 0.38  alm: 0.62  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 32.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U06712-1 is mit