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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U06660-1
Contig ID Contig-U06660-1
Contig update 2001. 8.30
Contig sequence 
>Contig-U06660-1 (Contig-U06660-1Q) /CSM_Contig/Contig-U06660-1Q.Seq.d
ATGCATGTTATTTTGGTAATGTTTTTACATGTAGAGATGATGGTAAAATT
ATATATGATATGTATGAAGGATATAATTGTAATGGTTCAATAGTGTTTAG
TATGGTAATTGAGAATGATGTTTGTGATACAAATTCATCTCAATCTTATC
CAACAGTATATTCATGTGTTGATAGTATTGAAATTCCAACAAGTGGTTTC
CTAGTTTATACATCACAAGACGATGATTGTGGTAGTAAAGAGGATTTAGT
CAATTTAGAAATCACTCCATTAAATACATGTATCAATGAAGATCAAACTT
ATTCATTCATTCAAACTTGTAATTCAACAAATTATTCAACTACAAAATAT
TTTGGTACTTCTTGTAAAGGTGGTCCAATTGGTCATGCTCAAGGTGAAAT
AACTTGTCGTCTAAATAATCCAAATCAATATTCTTCGCAATATGTTTGTC
TTTGAAAAAAAAAAAAAAAAAAAAAAAAAATAAACCAATAAAATAAAATA
CAAATTTAAAATATAA
Gap no gap
Contig length 516
Chromosome number (1..6, M) 1
Chromosome length 4919822
Start point 403647
End point 403221
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U06660
List of clone(s) 

est1=VSE774Z,1,517
Translated Amino Acid sequence 
ACYFGNVFTCRDDGKIIYDMYEGYNCNGSIVFSMVIENDVCDTNSSQSYPTVYSCVDSIE
IPTSGFLVYTSQDDDCGSKEDLVNLEITPLNTCINEDQTYSFIQTCNSTNYSTTKYFGTS
CKGGPIGHAQGEITCRLNNPNQYSSQYVCL*kkkkkkkkinq*nkiqi*ni
Translated Amino Acid sequence (All Frames) 
Frame A:
mhvilvmflhvemmvklymicmkdiivmvq*clvw*lrmmfviqihlnliqqyihvlivl
kfqqvvs*fihhktmivvvkri*si*kslh*ihvsmkiklihsfklviqqiiqlqnilvl
lvkvvqlvmlkvk*lvv*iiqinilrnmfvfekkkkkkkk*tnkikykfki*


Frame B:
cmlfw*cfym*r*w*nyi*yv*ri*l*wfnsv*ygn*e*cl*ykfisilsnsifmc**y*
nsnkwfpslyitrr*lw**rgfsqfrnhsikymyq*rsnlfihsnl*fnklfnykifwyf
l*rwsnwscsr*nnlssk*sksiffaiclslkkkkkkkknkpik*ntnlky


Frame C:
ACYFGNVFTCRDDGKIIYDMYEGYNCNGSIVFSMVIENDVCDTNSSQSYPTVYSCVDSIE
IPTSGFLVYTSQDDDCGSKEDLVNLEITPLNTCINEDQTYSFIQTCNSTNYSTTKYFGTS
CKGGPIGHAQGEITCRLNNPNQYSSQYVCL*kkkkkkkkinq*nkiqi*ni
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U06660-1 (Contig-U06660-1Q)
/CSM_Contig/Contig-U06660-1Q.Seq.d
         (516 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U06660-1 (Contig-U06660-1Q) /CSM_Contig/Conti...   900  0.0
Contig-U14814-1 (Contig-U14814-1Q) /CSM_Contig/Conti...    44  1e-04
Contig-U13949-1 (Contig-U13949-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U11850-1 (Contig-U11850-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U09219-1 (Contig-U09219-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U06537-1 (Contig-U06537-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U04763-1 (Contig-U04763-1Q) /CSM_Contig/Conti...    38  0.009
Contig-U14514-1 (Contig-U14514-1Q) /CSM_Contig/Conti...    36  0.036
Contig-U05027-1 (Contig-U05027-1Q) /CSM_Contig/Conti...    36  0.036
Contig-U00906-1 (Contig-U00906-1Q) /CSM_Contig/Conti...    36  0.036

>Contig-U06660-1 (Contig-U06660-1Q) /CSM_Contig/Contig-U06660-1Q.Seq.d
          Length = 516

 Score =  900 bits (454), Expect = 0.0
 Identities = 454/454 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   atgcatgttattttggtaatgtttttacatgtagagatgatggtaaaattatatatgata 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   atgcatgttattttggtaatgtttttacatgtagagatgatggtaaaattatatatgata 60

                                                                       
Query: 61  tgtatgaaggatataattgtaatggttcaatagtgtttagtatggtaattgagaatgatg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tgtatgaaggatataattgtaatggttcaatagtgtttagtatggtaattgagaatgatg 120

                                                                       
Query: 121 tttgtgatacaaattcatctcaatcttatccaacagtatattcatgtgttgatagtattg 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tttgtgatacaaattcatctcaatcttatccaacagtatattcatgtgttgatagtattg 180

                                                                       
Query: 181 aaattccaacaagtggtttcctagtttatacatcacaagacgatgattgtggtagtaaag 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 aaattccaacaagtggtttcctagtttatacatcacaagacgatgattgtggtagtaaag 240

                                                                       
Query: 241 aggatttagtcaatttagaaatcactccattaaatacatgtatcaatgaagatcaaactt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 aggatttagtcaatttagaaatcactccattaaatacatgtatcaatgaagatcaaactt 300

                                                                       
Query: 301 attcattcattcaaacttgtaattcaacaaattattcaactacaaaatattttggtactt 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attcattcattcaaacttgtaattcaacaaattattcaactacaaaatattttggtactt 360

                                                                       
Query: 361 cttgtaaaggtggtccaattggtcatgctcaaggtgaaataacttgtcgtctaaataatc 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 cttgtaaaggtggtccaattggtcatgctcaaggtgaaataacttgtcgtctaaataatc 420

                                             
Query: 421 caaatcaatattcttcgcaatatgtttgtctttg 454
           ||||||||||||||||||||||||||||||||||
Sbjct: 421 caaatcaatattcttcgcaatatgtttgtctttg 454


 Score = 71.9 bits (36), Expect = 7e-13
 Identities = 36/36 (100%)
 Strand = Plus / Plus

                                               
Query: 481 taaaccaataaaataaaatacaaatttaaaatataa 516
           ||||||||||||||||||||||||||||||||||||
Sbjct: 481 taaaccaataaaataaaatacaaatttaaaatataa 516


>Contig-U14814-1 (Contig-U14814-1Q) /CSM_Contig/Contig-U14814-1Q.Seq.d
          Length = 185

 Score = 44.1 bits (22), Expect = 1e-04
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 490 aaaataaaatacaaatttaaaa 511
           ||||||||||||||||||||||
Sbjct: 151 aaaataaaatacaaatttaaaa 130


>Contig-U13949-1 (Contig-U13949-1Q) /CSM_Contig/Contig-U13949-1Q.Seq.d
          Length = 1211

 Score = 38.2 bits (19), Expect = 0.009
 Identities = 19/19 (100%)
 Strand = Plus / Minus

                              
Query: 486 caataaaataaaatacaaa 504
           |||||||||||||||||||
Sbjct: 479 caataaaataaaatacaaa 461


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 10,150
Number of Sequences: 6905
Number of extensions: 10150
Number of successful extensions: 1881
Number of sequences better than 10.0: 652
length of query: 516
length of database: 5,674,871
effective HSP length: 16
effective length of query: 500
effective length of database: 5,564,391
effective search space: 2782195500
effective search space used: 2782195500
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2008.11. 9
Homology vs DNA 
Query= Contig-U06660-1 (Contig-U06660-1Q) /CSM_Contig/Contig-U06660-1Q.Seq.d
         (516 letters)

Database: ddbj_A
           92,845,959 sequences; 95,242,211,685 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU263367) Dictyostelium discoideum vegetative cDNA clone:VS...   900   0.0    1
(AC225673) Procavia capensis clone CH280-104B22, WORKING DRA...    38   0.013  2
(AM451422) Vitis vinifera, whole genome shotgun sequence, co...    48   0.31   1
(CU928731) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.31   1
(CT418502) Sus scrofa genomic clone CH242-478A12, genomic su...    48   0.31   1
(CZ009671) OA_BBa0082C21.f OA_BBa Oryza alta genomic clone O...    36   0.87   2
(AC015813) Homo sapiens, clone RP11-159D12, complete sequence.     46   1.2    1
(AC005962) Homo sapiens chromosome 17, clone hRPK.506_H_21, ...    46   1.2    1
(AC004757) Homo sapiens chromosome 17, clone CTB-117K16, com...    46   1.2    1
(AC111394) Rattus norvegicus clone CH230-149M18, *** SEQUENC...    46   1.2    1
(AC095336) Rattus norvegicus clone CH230-121C8, *** SEQUENCI...    46   1.2    1
(EK039581) 1092959502896 Global-Ocean-Sampling_GS-31-01-01-1...    46   1.2    1
(EK018317) 1092955254876 Global-Ocean-Sampling_GS-31-01-01-1...    46   1.2    1
(AC118612) Mus musculus chromosome 6 clone RP23-238G8 map 6,...    44   1.4    3
(AC124188) Mus musculus BAC clone RP23-240C17 from 6, comple...    44   1.5    3
(CR456629) Zebrafish DNA sequence from clone DKEY-42B22 in l...    32   2.4    2
(CR392346) Zebrafish DNA sequence from clone DKEY-15G17 in l...    32   2.4    2
(AC206031) Zea mays chromosome 1 clone CH201-237B6; ZMMBBc02...    32   2.4    2
(AC216185) Zea mays chromosome 9 clone CH201-13C8; ZMMBBc001...    32   2.4    2
(AC203307) Zea mays chromosome 6 clone CH201-58L5; ZMMBBc005...    32   2.4    2
(AC200257) Zea mays chromosome 10 clone CH201-3C1; ZMMBBc000...    32   2.4    2
(AC210260) Zea mays chromosome 5 clone CH201-44F4; ZMMBBc004...    32   2.4    2
(AC211173) Zea mays chromosome 1 clone CH201-70O19; ZMMBBc00...    32   2.4    2
(BX928755) Zebrafish DNA sequence from clone DKEY-199C16 in ...    32   2.4    2
(AC211535) Zea mays chromosome 5 clone ZMMBBb-223D21; ZMMBBb...    32   2.4    2
(AC189770) Zea mays chromosome 9 clone CH201-305O17; ZMMBBc0...    32   2.4    2
(AC199117) Zea mays chromosome 10 clone ZMMBBb-215G4; ZMMBBb...    32   2.4    2
(AC217819) Zea mays chromosome 6 clone ZMMBBb-160K10; ZMMBBb...    32   2.5    2
(AB178773) Lingula anatina mitochondrial cox1, cox2, nad2, n...    32   2.7    2
(CC603616) OGPAP76TV ZM_0.7_1.5_KB Zea mays genomic clone ZM...    32   3.2    2
(CG175497) PUFMH83TB ZM_0.6_1.0_KB Zea mays genomic clone ZM...    32   3.2    2
(CG345488) OGWBF67TV ZM_0.7_1.5_KB Zea mays genomic clone ZM...    32   3.2    2
(CC603606) OGPAP76TH ZM_0.7_1.5_KB Zea mays genomic clone ZM...    32   3.2    2
(CT244800) Sus scrofa genomic clone CH242-268P14, genomic su...    32   3.2    2
(AC160204) Bos taurus clone CH240-82N6, WORKING DRAFT SEQUEN...    42   4.4    2
(AC126259) Mus musculus BAC clone RP23-330F19 from chromosom...    44   4.9    1
(AC192739) Pan troglodytes BAC clone CH251-190L8 from chromo...    44   4.9    1
(AC145882) Pan troglodytes BAC clone RP43-7E19 from chromoso...    44   4.9    1
(AC144805) Medicago truncatula clone mth2-72a11, complete se...    44   4.9    1
(AC145350) Homo sapiens chromosome 16 clone RP11-19N8, compl...    44   4.9    1
(AC140873) Homo sapiens chromosome 16 clone RP11-1161D4, com...    44   4.9    1
(AC140862) Homo sapiens chromosome 16 clone RP11-1057D24, co...    44   4.9    1
(AC138916) Homo sapiens chromosome 16 clone RP11-629J1, comp...    44   4.9    1
(AC138869) Homo sapiens chromosome 16 clone RP11-1437A8, com...    44   4.9    1
(AC138815) Homo sapiens chromosome 16 clone RP11-1089D10, co...    44   4.9    1
(AC114794) Homo sapiens BAC clone RP11-680G19 from 2, comple...    44   4.9    1
(AC104072) Homo sapiens BAC clone RP11-320H14 from 4, comple...    44   4.9    1
(AC093745) Homo sapiens BAC clone RP11-25B15 from 4, complet...    44   4.9    1
(AC078815) Homo sapiens 12q BAC RP11-766N7 (Roswell Park Can...    44   4.9    1
(AC145317) Homo sapiens chromosome 16 clone RP11-1222K13, WO...    44   4.9    1
(AC145315) Homo sapiens chromosome 16 clone RP11-822C7, WORK...    44   4.9    1
(AC141610) Homo sapiens chromosome 16 clone RP11-780N8, WORK...    44   4.9    1
(AC140900) Homo sapiens chromosome 16 clone RP11-792N21, WOR...    44   4.9    1
(AC140144) Homo sapiens chromosome 5 clone RP11-1429M18, WOR...    44   4.9    1
(AC140142) Homo sapiens chromosome 5 clone RP11-1425D10, WOR...    44   4.9    1
(AC139826) Homo sapiens chromosome 16 clone RP11-563G9, WORK...    44   4.9    1
(AC138861) Homo sapiens chromosome 16 clone RP11-1378C7, WOR...    44   4.9    1
(AC138797) Homo sapiens chromosome 16 clone CTD-2507K12, WOR...    44   4.9    1
(AC138796) Homo sapiens chromosome 16 clone CTD-2383C17, WOR...    44   4.9    1
(AC104064) Homo sapiens chromosome 2 clone RP11-44F16, WORKI...    44   4.9    1
(AC069245) Homo sapiens chromosome 12 clone RP11-757N13, WOR...    44   4.9    1
(AC027295) Homo sapiens chromosome 12 clone RP11-293C20, WOR...    44   4.9    1
(AC229718) Medicago truncatula clone mth2-80d21, WORKING DRA...    44   4.9    1
(AC222096) Bos taurus clone CH240-371P12, WORKING DRAFT SEQU...    44   4.9    1
(AC185394) Felis catus clone RP86-271A16, WORKING DRAFT SEQU...    44   4.9    1
(AC020623) Homo sapiens chromosome 12 clone RP11-87A24, WORK...    44   4.9    1
(AC016726) Homo sapiens chromosome 12 clone RP11-341K11, WOR...    44   4.9    1
(AC016472) Homo sapiens clone RP11-20P1, WORKING DRAFT SEQUE...    44   4.9    1
(AC009419) Homo sapiens clone RP11-44J2, WORKING DRAFT SEQUE...    44   4.9    1
(EJ466727) 1095403325287 Global-Ocean-Sampling_GS-28-01-01-1...    44   4.9    1
(FF563266) TT1B379TH Tetrahymena thermophila SB210 cDNA libr...    44   4.9    1
(CP000962) Clostridium botulinum A3 str. Loch Maree, complet...    44   4.9    1
(AC223362) Bos taurus clone CH240-420M24, WORKING DRAFT SEQU...    42   5.2    2
(AL732291) Mouse DNA sequence from clone RP23-48I14 on chrom...    36   5.3    6
(AC107988) Homo sapiens chromosome 18, clone CTC-346I21, com...    34   5.6    3
(AC098135) Rattus norvegicus clone CH230-163M15, *** SEQUENC...    34   5.8    6
(AC120049) Homo sapiens chromosome 18, clone RP11-456K23, co...    34   6.4    3
(CR538741) Zebrafish DNA sequence from clone DKEY-97J15 in l...    34   6.5    5
(AC164507) Loxodonta africana clone VMRC15-288H10, WORKING D...    42   6.9    3
(AL353730) Human DNA sequence from clone RP11-55P9 on chromo...    40   7.1    4
(CT573069) Zebrafish DNA sequence from clone CH73-386I5 in l...    38   7.2    3
(CU468498) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    34   7.6    5
(AC225293) Monodelphis domestica clone VMRC18-786C19, WORKIN...    38   8.5    3
(AC193339) Zea mays chromosome 7 clone CH201-177O17; ZMMBBc0...    32   8.8    2
(BX548250) Zebrafish DNA sequence from clone DKEYP-104H9 in ...    30   8.8    2
(AC159145) Medicago truncatula chromosome 2 BAC clone mth2-6...    32   9.0    2
(AP007771) Lotus japonicus genomic DNA, chromosome 4, clone:...    42   9.8    6

>(AU263367) Dictyostelium discoideum vegetative cDNA clone:VSE774,
           3' end single read.
          Length = 517

 Score =  900 bits (454), Expect = 0.0
 Identities = 454/454 (100%)
 Strand = Plus / Plus


Query: 1   atgcatgttattttggtaatgtttttacatgtagagatgatggtaaaattatatatgata 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   atgcatgttattttggtaatgtttttacatgtagagatgatggtaaaattatatatgata 60


Query: 61  tgtatgaaggatataattgtaatggttcaatagtgtttagtatggtaattgagaatgatg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  tgtatgaaggatataattgtaatggttcaatagtgtttagtatggtaattgagaatgatg 120


Query: 121 tttgtgatacaaattcatctcaatcttatccaacagtatattcatgtgttgatagtattg 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tttgtgatacaaattcatctcaatcttatccaacagtatattcatgtgttgatagtattg 180


Query: 181 aaattccaacaagtggtttcctagtttatacatcacaagacgatgattgtggtagtaaag 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 aaattccaacaagtggtttcctagtttatacatcacaagacgatgattgtggtagtaaag 240


Query: 241 aggatttagtcaatttagaaatcactccattaaatacatgtatcaatgaagatcaaactt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 aggatttagtcaatttagaaatcactccattaaatacatgtatcaatgaagatcaaactt 300


Query: 301 attcattcattcaaacttgtaattcaacaaattattcaactacaaaatattttggtactt 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attcattcattcaaacttgtaattcaacaaattattcaactacaaaatattttggtactt 360


Query: 361 cttgtaaaggtggtccaattggtcatgctcaaggtgaaataacttgtcgtctaaataatc 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 cttgtaaaggtggtccaattggtcatgctcaaggtgaaataacttgtcgtctaaataatc 420


Query: 421 caaatcaatattcttcgcaatatgtttgtctttg 454
           ||||||||||||||||||||||||||||||||||
Sbjct: 421 caaatcaatattcttcgcaatatgtttgtctttg 454

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 92845959
Number of Hits to DB: 537,100,158
Number of extensions: 32424398
Number of successful extensions: 3097982
Number of sequences better than 10.0: 87
Length of query: 516
Length of database: 95,242,211,685
Length adjustment: 23
Effective length of query: 493
Effective length of database: 93,106,754,628
Effective search space: 45901630031604
Effective search space used: 45901630031604
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 6.22
Homology vs Protein 
Query= Contig-U06660-1 (Contig-U06660-1Q) /CSM_Contig/Contig-U06660-1Q.Seq.d
         (516 letters)

Database: nrp_A
           3,204,285 sequences; 1,040,966,779 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116963_41(AC116963|pid:none) Dictyostelium discoideum chromoso...    58   1e-07
AC115612_7(AC115612|pid:none) Dictyostelium discoideum chromosom...    46   5e-04
AC115584_2(AC115584|pid:none) Dictyostelium discoideum chromosom...    42   0.007
AC115584_4(AC115584|pid:none) Dictyostelium discoideum chromosom...    41   0.020
CP000496_129(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...    37   0.37
AF065168_1(AF065168|pid:none) Giardia intestinalis variant-speci...    36   0.48
EF538805_1(EF538805|pid:none) Trypanoplasma borreli cathepsin L ...    35   0.83
AK128348_1(AK128348|pid:none) Homo sapiens cDNA FLJ46490 fis, cl...    33   3.2
FN357669_3(FN357669|pid:none) Schistosoma mansoni genome sequenc...    33   4.1
CR380947_51(CR380947|pid:none) Candida glabrata strain CBS138 ch...    33   5.4
T21442(T21442) hypothetical protein F26H9.6 - Caenorhabditis ele...    32   7.0
U80927_1(U80927|pid:none) Dictyostelium discoideum unknown prote...    32   7.1
AC115607_13(AC115607|pid:none) Dictyostelium discoideum chromoso...    32   9.1
BC155302_1(BC155302|pid:none) Danio rerio zgc:174946, mRNA (cDNA...    32   9.1
BC128867_1(BC128867|pid:none) Danio rerio zgc:158427, mRNA (cDNA...    32   9.1
BC026828_1(BC026828|pid:none) Mus musculus RIKEN cDNA 2210415F13...    32   9.1
(Q58269) RecName: Full=Uncharacterized protein MJ0859;  &C64407(...    32   9.1
CP000609_84(CP000609|pid:none) Methanococcus maripaludis C5, com...    32   9.1
(Q55DH0) RecName: Full=PI-PLC X domain-containing protein DDB_G0...    32   9.3

>AC116963_41(AC116963|pid:none) Dictyostelium discoideum chromosome
           2 map 4657875-4914984 strain AX4, complete sequence.
          Length = 201

 Score = 58.2 bits (139), Expect = 1e-07
 Identities = 39/143 (27%), Positives = 60/143 (41%), Gaps = 6/143 (4%)
 Frame = +3

Query: 21  VFTC-RDDGKIIYDMYEGYNCNGSIVFSMVIENDVCDTNSSQSYPTVYSCVDSIEIPTSG 197
           ++TC   +  I    Y G NC G    S+ +E + C  +   S   +YSC +  ++ T+
Sbjct: 56  MYTCDAANNNITRTQYYGSNCQGGSE-SINLEANKCTNDFGTSNNVIYSCTEDYDLGTNS 114

Query: 198 FLVYTSQ----DDDCGSKEDLVNLEITPLNTCINEDQTYSFIQTCNSTNYSTTKYFGT-S 362
            + Y +     D +   K+D++ +    LN C   D       +CN T+Y    YF T S
Sbjct: 115 LVEYLTNGQCPDSNPAWKDDIIQVASLGLNICTYTDVGSIKTTSCNETSYDENYYFSTDS 174

Query: 363 CKGGPIGHAQGEITCRLNNPNQY 431
           C      HA     C  N P  Y
Sbjct: 175 CTNAIFSHALEFPECSQNTPEIY 197

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 664,947,576
Number of extensions: 12137689
Number of successful extensions: 28136
Number of sequences better than 10.0: 19
Number of HSP's gapped: 28080
Number of HSP's successfully gapped: 19
Length of query: 172
Length of database: 1,040,966,779
Length adjustment: 119
Effective length of query: 53
Effective length of database: 659,656,864
Effective search space: 34961813792
Effective search space used: 34961813792
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)
PSORT 

 psg: 0.44  gvh: 0.34  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U06660-1 is nuc
VS (DIR, S) 1
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0