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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U01226-1
Contig ID Contig-U01226-1
Contig update 2002. 9.13
Contig sequence 
>Contig-U01226-1 (Contig-U01226-1Q) /CSM_Contig/Contig-U01226-1Q.Seq.d
AGACATCTCTCGTTACATTCCAGTAATTCGTATCAATTGGAGTGAATTTG
TTGACCCTGAACAATTGGCTCAAATGATTAAAGCTGAATATGAATCAATG
AGATTTATGAATCAAATTAATCCTCCAACTTTTGGTAATGGTCCAACCAC
TAATAAAATTATATCAACTCCAAAAGATTTA
Gap no gap
Contig length 181
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 418208
End point 418389
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U01226
List of clone(s) 

est1=SLD559F,1,182
Translated Amino Acid sequence 
DISRYIPVIRINWSEFVDPEQLAQMIKAEYESMRFMNQINPPTFGNGPTTNKIISTPKDL
Translated Amino Acid sequence (All Frames) 
Frame A:
rhlslhssnsyqle*ic*p*tigsnd*s*i*ineiyesn*ssnfw*wsnh**nyinskrf


Frame B:
DISRYIPVIRINWSEFVDPEQLAQMIKAEYESMRFMNQINPPTFGNGPTTNKIISTPKDL


Frame C:
tslvtfq*fvsigvnlltlnnwlk*lklnmnq*dl*iklilqllvmvqpliklyqlqki
own update 2004. 6. 7
Homology vs CSM-cDNA 
Query= Contig-U01226-1 (Contig-U01226-1Q)
/CSM_Contig/Contig-U01226-1Q.Seq.d
         (181 letters)

Database: CSM
           8361 sequences; 7,895,291 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U01226-1 (Contig-U01226-1Q) /CSM_Contig/Conti...   359  e-100
Contig-U12546-1 (Contig-U12546-1Q) /CSM_Contig/Conti...    36  0.017
Contig-U06985-1 (Contig-U06985-1Q) /CSM_Contig/Conti...    36  0.017
Contig-U12246-1 (Contig-U12246-1Q) /CSM_Contig/Conti...    34  0.066
Contig-U11155-1 (Contig-U11155-1Q) /CSM_Contig/Conti...    34  0.066
Contig-U10397-1 (Contig-U10397-1Q) /CSM_Contig/Conti...    34  0.066
Contig-U02713-1 (Contig-U02713-1Q) /CSM_Contig/Conti...    34  0.066
Contig-U11258-1 (Contig-U11258-1Q) /CSM_Contig/Conti...    32  0.26
Contig-U06617-1 (Contig-U06617-1Q) /CSM_Contig/Conti...    32  0.26
Contig-U06500-1 (Contig-U06500-1Q) /CSM_Contig/Conti...    32  0.26

>Contig-U01226-1 (Contig-U01226-1Q) /CSM_Contig/Contig-U01226-1Q.Seq.d
          Length = 181

 Score =  359 bits (181), Expect = e-100
 Identities = 181/181 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   agacatctctcgttacattccagtaattcgtatcaattggagtgaatttgttgaccctga 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   agacatctctcgttacattccagtaattcgtatcaattggagtgaatttgttgaccctga 60

                                                                       
Query: 61  acaattggctcaaatgattaaagctgaatatgaatcaatgagatttatgaatcaaattaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  acaattggctcaaatgattaaagctgaatatgaatcaatgagatttatgaatcaaattaa 120

                                                                       
Query: 121 tcctccaacttttggtaatggtccaaccactaataaaattatatcaactccaaaagattt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tcctccaacttttggtaatggtccaaccactaataaaattatatcaactccaaaagattt 180

            
Query: 181 a 181
           |
Sbjct: 181 a 181


>Contig-U12546-1 (Contig-U12546-1Q) /CSM_Contig/Contig-U12546-1Q.Seq.d
          Length = 1365

 Score = 36.2 bits (18), Expect = 0.017
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 144 caaccactaataaaatta 161
           ||||||||||||||||||
Sbjct: 928 caaccactaataaaatta 945


>Contig-U06985-1 (Contig-U06985-1Q) /CSM_Contig/Contig-U06985-1Q.Seq.d
          Length = 561

 Score = 36.2 bits (18), Expect = 0.017
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                             
Query: 83  gctgaatatgaatcaatg 100
           ||||||||||||||||||
Sbjct: 368 gctgaatatgaatcaatg 385


  Database: CSM
    Posted date:  Jun 7, 2004  8:33 AM
  Number of letters in database: 7,895,291
  Number of sequences in database:  8361
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 3474
Number of Sequences: 8361
Number of extensions: 3474
Number of successful extensions: 1038
Number of sequences better than 10.0: 89
length of query: 181
length of database: 7,895,291
effective HSP length: 15
effective length of query: 166
effective length of database: 7,769,876
effective search space: 1289799416
effective search space used: 1289799416
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2009. 4.25
Homology vs DNA 
Query= Contig-U01226-1 (Contig-U01226-1Q) /CSM_Contig/Contig-U01226-1Q.Seq.d
         (181 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AY192983) Dictyostelium discoideum deoxyadenosine kinase (D...   359   2e-95  1
(AU061221) Dictyostelium discoideum slug cDNA, clone SLD559.      359   2e-95  1
(CR380956) Candida glabrata strain CBS138 chromosome J compl...    56   4e-04  1
(EJ556457) 1092959503465 Global-Ocean-Sampling_GS-29-01-01-1...    40   0.074  2
(ES525922) BIG_AF_33742 Brine Shrimp embryos, 15 hours after...    42   0.16   2
(AM426216) Vitis vinifera contig VV78X104889.4, whole genome...    44   1.7    1
(EK429303) 1095516108060 Global-Ocean-Sampling_GS-31-01-01-1...    44   1.7    1
(CA138633) SCEQRT2028H12.g RT2 Saccharum officinarum cDNA cl...    44   1.7    1
(BY464729) Mus musculus adult male liver cDNA, RIKEN full-le...    36   1.9    2
(AC064841) Homo sapiens BAC clone CTD-2015A10 from 2, comple...    32   3.4    2
(EJ117131) 1092343357792 Global-Ocean-Sampling_GS-27-01-01-1...    40   3.7    2
(EJ205047) 1092344334688 Global-Ocean-Sampling_GS-27-01-01-1...    40   4.1    2
(FF898222) CBWU31736.g1 Yutaka Satou unpublished cDNA librar...    34   4.4    2
(BW080322) Ciona intestinalis cDNA, clone:rcieg081o04, 3' en...    34   4.4    2
(AG107078) Pan troglodytes DNA, clone: PTB-111M15.R.               32   4.4    2
(FF759365) XABT65784.rev Gateway compatible cien cDNA librar...    34   4.4    2
(FF717348) XABT35715.rev Gateway compatible cien cDNA librar...    34   4.5    2
(CR354564) Zebrafish DNA sequence from clone CH211-51A12 in ...    42   6.6    1
(BX942812) Zebrafish DNA sequence from clone DKEY-7N6 in lin...    42   6.6    1
(AC012310) Homo sapiens chromosome 19 clone CTC-518O7, compl...    42   6.6    1
(AC126896) Rattus norvegicus clone CH230-300E3, *** SEQUENCI...    42   6.6    1
(AC118968) Rattus norvegicus clone CH230-184P18, *** SEQUENC...    42   6.6    1
(AC095607) Rattus norvegicus clone CH230-8N21, WORKING DRAFT...    42   6.6    1
(AC176048) Strongylocentrotus purpuratus clone R3-3024F6, WO...    42   6.6    1
(ER547091) 1093016187703 Global-Ocean-Sampling_GS-35-01-01-1...    42   6.6    1
(DN321571) LIB3171-065-Q1-K1-D7 LIB3171 Canis lupus familiar...    42   6.6    1
(CU071818) AGENAE Rainbow trout multi-tissues library (tcce)...    42   6.6    1
(BU333943) 603499117F1 CSEQCHN65 Gallus gallus cDNA clone Ch...    42   6.6    1
(ES523275) BIG_AF_31095 Brine Shrimp embryos, 15 hours after...    42   6.6    1
(ES510763) BIG_AF_18580 Brine Shrimp embryos, 10 hours after...    42   6.6    1
(ES509147) BIG_AF_16964 Brine Shrimp embryos, 10 hours after...    42   6.6    1
(AP004746) Oryza sativa Japonica Group genomic DNA, chromoso...    38   7.2    4
(AC178855) Strongylocentrotus purpuratus clone R3-26H8, WORK...    40   9.8    2

>(AY192983) Dictyostelium discoideum deoxyadenosine kinase (DAK)
           mRNA, complete cds.
          Length = 738

 Score =  359 bits (181), Expect = 2e-95
 Identities = 181/181 (100%)
 Strand = Plus / Plus


Query: 1   agacatctctcgttacattccagtaattcgtatcaattggagtgaatttgttgaccctga 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 555 agacatctctcgttacattccagtaattcgtatcaattggagtgaatttgttgaccctga 614


Query: 61  acaattggctcaaatgattaaagctgaatatgaatcaatgagatttatgaatcaaattaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 615 acaattggctcaaatgattaaagctgaatatgaatcaatgagatttatgaatcaaattaa 674


Query: 121 tcctccaacttttggtaatggtccaaccactaataaaattatatcaactccaaaagattt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 675 tcctccaacttttggtaatggtccaaccactaataaaattatatcaactccaaaagattt 734


Query: 181 a 181
           |
Sbjct: 735 a 735

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 211,662,569
Number of extensions: 12088619
Number of successful extensions: 3288884
Number of sequences better than 10.0: 33
Length of query: 181
Length of database: 101,790,757,118
Length adjustment: 22
Effective length of query: 159
Effective length of database: 99,544,435,898
Effective search space: 15827565307782
Effective search space used: 15827565307782
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)
protein update 2009. 7.14
Homology vs Protein 
Query= Contig-U01226-1 (Contig-U01226-1Q) /CSM_Contig/Contig-U01226-1Q.Seq.d
         (181 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YL2) RecName: Full=Deoxyadenosine kinase;          EC=2.7.1....   126   2e-28

>(Q54YL2) RecName: Full=Deoxyadenosine kinase;          EC=2.7.1.76;
           AltName: Full=DddDAK;          Short=DAK;
           &AY192983_1(AY192983|pid:none)
          Length = 245

 Score =  126 bits (317), Expect = 2e-28
 Identities = 60/60 (100%), Positives = 60/60 (100%)
 Frame = +2

Query: 2   DISRYIPVIRINWSEFVDPEQLAQMIKAEYESMRFMNQINPPTFGNGPTTNKIISTPKDL 181
           DISRYIPVIRINWSEFVDPEQLAQMIKAEYESMRFMNQINPPTFGNGPTTNKIISTPKDL
Sbjct: 186 DISRYIPVIRINWSEFVDPEQLAQMIKAEYESMRFMNQINPPTFGNGPTTNKIISTPKDL 245

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 282,514,342
Number of extensions: 5205659
Number of successful extensions: 8508
Number of sequences better than 10.0: 1
Number of HSP's gapped: 8507
Number of HSP's successfully gapped: 1
Length of query: 60
Length of database: 1,051,180,864
Length adjustment: 32
Effective length of query: 28
Effective length of database: 947,610,976
Effective search space: 26533107328
Effective search space used: 26533107328
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 26 (14.6 bits)
PSORT 

 psg: 0.65  gvh: 0.34  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	 12.0 %: mitochondrial

>> prediction for Contig-U01226-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 1
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0