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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U01123-1
Contig ID Contig-U01123-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U01123-1 (Contig-U01123-1Q) /CSM_Contig/Contig-U01123-1Q.Seq.d
AAACTCAATTGAAAAAATTAATTGAAAAACTTCCAATTTCTTCAGCTTCT
TCTTCAACTTCAACATCTTCACCTTCTTCTTTGTTAGAATCAAATTTACT
AATTCATCAAAATGATACCCACAATAATAATCATCATCATCAAGATGAAA
TAACAAAATCTGAAATCAAAGTTGCTTCACCTTTAATAAAACAAGAATTA
AGACAATCTCCAAAACAACTTGAAAGAAATTCTCCACAAAATGATAAAGA
ATTAACAATTGATGAAAGTAATGATAGTATTGATACTACCAATATTGGTA
GAAAAAGTAATAATAATAATATTAAAAATAATAATAATAATAATAATATA
AATAATAGTAATAATAATAATAATAATAGCAATCAAGTAATTGTCAAAAA
AGATAATAAAAAACTCATTGATGAAGATGAAGATAATGATCAACCTACAA
CAATTTCAACATCAATAATAAAAAATGATGATGATAAGAATGACCAAATG
AAATTGAAAATAACATTCTCAAAGAAATCATCTGATCTTAACTTAACAAC
AAGTAGTGACTCATCCAATCCAACAGTTGTATCTCCGATGGCGAACGATG
ATGCTGAGACTGGAAGGAAGGAAATTATTAAAAAAAAAAAGAAAAAAAAA
AAAAAAAAAAAGAATAAAGTAATAAAAAAAATATTTATTTGAA-------
---GAGAATTCGATAGAGTAATTACCATTCAATCAAACAACTATGAAAAA
CTATTATCAAAGAAATTTCATGATATGAAAAAAGATTTAAATGAAAACAC
TGATGCCCTATCAACTTCATTTGATATTCTTGATAATATTTTAATTAGTC
AAACTTTACAACATCAACAAATGCAACAACATTATAAACAACAACAACAA
TTTCAACAACAACAACAGCAACATCACAACCAACAACAACCATTTCAACC
AATCAACCTCCCTCCTGTTTACAATAACTCTTCAAATTTTAATTCCAATA
ATTCAATGAATCAATACTCATATAATAATTATCATCACCACCAACAACAA
CAACAACAACAACAGCAAAACAATCAACAAAATCAAAAGAAAACCAAAAT
ATTTCAATAATTAATAAAAAGAGAAAAAA
Gap gap included
Contig length 1119
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 2002594
End point 2001462
Strand (PLUS/MINUS) MINUS
Number of clones 2
Number of EST 3
Link to clone list U01123
List of clone(s) 

est1=SLB332F,1,350
est2=SLI342E,201,694
est3=SLB332Z,695,1121
Translated Amino Acid sequence 
nsiekin*ktsnffsfffnfniftfffvrikftnssk*ypq**ssssr*nnki*nqscft
fnktriktisktt*kkfstk**rinn**k***y*yyqyw*kk****y*k******yk***
******qssncqkr**kth**r*r**stynnfninnkk****e*pneiennilkeii*s*
lnnk**liqsnscisdger*c*dwkegny*kkkekkkkkke*SNKKNIYL---

---EFDRVITIQSNNYEKLLSKKFHDMKKDLNENTDALSTSFDILDNILISQTLQHQQMQ
QHYKQQQQFQQQQQQHHNQQQPFQPINLPPVYNNSSNFNSNNSMNQYSYNNYHHHQQQQQ
QQQQNNQQNQKKTKIFQ*likrek
Translated Amino Acid sequence (All Frames) 
Frame A:
kln*kn*lknfqflqlllqlqhlhlllc*nqiy*fikmiptiiiiiikmk*qnlkskllh
l**nkn*dnlqnnlkeilhkmikn*qlmkvmivlilpilvekviiiilkiiiiiii*iiv
iiiiiiaik*lskkiiknslmkmkiminlqqfqhq**kmmmirmtk*n*k*hsqrnhlil
t*qqvvthpiqqlylrwrtmmlrlegrkllkkkrkkkkkkrik**kkylfe---

---ensie*lpfnqttmknyyqrnfmi*kki*mktlmpyqlhlifliif*lvklyninkc
nniinnnnnfnnnnsnittnnnhfnqstsllftitlqilipiiq*inthiiiiittnnnn
nnnsktinkikrkpkyfnn**kekk

Frame B:
nsiekin*ktsnffsfffnfniftfffvrikftnssk*ypq**ssssr*nnki*nqscft
fnktriktisktt*kkfstk**rinn**k***y*yyqyw*kk****y*k******yk***
******qssncqkr**kth**r*r**stynnfninnkk****e*pneiennilkeii*s*
lnnk**liqsnscisdger*c*dwkegny*kkkekkkkkke*SNKKNIYL---

---rir*snyhsikql*ktiikeis*yekrfk*kh*cpinfi*ys**yfn*snfttstna
ttl*ttttisttttatsqptttistnqppsclq*lfkf*fq*fnesili**lsspptttt
tttakqstkskenqnisiinkkrk

Frame C:
tqlkklieklpissassststsspssllesnllihqndthnnnhhhqdeitkseikvasp
likqelrqspkqlernspqndkeltidesndsidttnigrksnnnniknnnnnnninnsn
nnnnnsnqvivkkdnkklidededndqpttistsiikndddkndqmklkitfskkssdln
lttssdssnptvvspmanddaetgrkeiikkkkkkkkkkknkvikkifi*---

---EFDRVITIQSNNYEKLLSKKFHDMKKDLNENTDALSTSFDILDNILISQTLQHQQMQ
QHYKQQQQFQQQQQQHHNQQQPFQPINLPPVYNNSSNFNSNNSMNQYSYNNYHHHQQQQQ
QQQQNNQQNQKKTKIFQ*likrek
own update 2004. 6. 7
Homology vs CSM-cDNA 
Query= Contig-U01123-1 (Contig-U01123-1Q)
/CSM_Contig/Contig-U01123-1Q.Seq.d
         (1129 letters)

Database: CSM
           8361 sequences; 7,895,291 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U01123-1 (Contig-U01123-1Q) /CSM_Contig/Conti...   498  e-140
Contig-U12907-1 (Contig-U12907-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U11017-1 (Contig-U11017-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U02881-1 (Contig-U02881-1Q) /CSM_Contig/Conti...    44  5e-04
Contig-U11491-1 (Contig-U11491-1Q) /CSM_Contig/Conti...    40  0.007
Contig-U10787-1 (Contig-U10787-1Q) /CSM_Contig/Conti...    40  0.007
Contig-U08850-1 (Contig-U08850-1Q) /CSM_Contig/Conti...    40  0.007
Contig-U06780-1 (Contig-U06780-1Q) /CSM_Contig/Conti...    40  0.007
Contig-U06747-1 (Contig-U06747-1Q) /CSM_Contig/Conti...    40  0.007
Contig-U12156-1 (Contig-U12156-1Q) /CSM_Contig/Conti...    38  0.028

>Contig-U01123-1 (Contig-U01123-1Q) /CSM_Contig/Contig-U01123-1Q.Seq.d
          Length = 1129

 Score =  498 bits (251), Expect = e-140
 Identities = 251/251 (100%)
 Strand = Plus / Plus

                                                                       
Query: 379 gcaatcaagtaattgtcaaaaaagataataaaaaactcattgatgaagatgaagataatg 438
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 379 gcaatcaagtaattgtcaaaaaagataataaaaaactcattgatgaagatgaagataatg 438

                                                                       
Query: 439 atcaacctacaacaatttcaacatcaataataaaaaatgatgatgataagaatgaccaaa 498
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 439 atcaacctacaacaatttcaacatcaataataaaaaatgatgatgataagaatgaccaaa 498

                                                                       
Query: 499 tgaaattgaaaataacattctcaaagaaatcatctgatcttaacttaacaacaagtagtg 558
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 499 tgaaattgaaaataacattctcaaagaaatcatctgatcttaacttaacaacaagtagtg 558

                                                                       
Query: 559 actcatccaatccaacagttgtatctccgatggcgaacgatgatgctgagactggaagga 618
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 559 actcatccaatccaacagttgtatctccgatggcgaacgatgatgctgagactggaagga 618

                      
Query: 619 aggaaattatt 629
           |||||||||||
Sbjct: 619 aggaaattatt 629


 Score =  448 bits (226), Expect = e-126
 Identities = 226/226 (100%)
 Strand = Plus / Plus

                                                                       
Query: 82  tgttagaatcaaatttactaattcatcaaaatgatacccacaataataatcatcatcatc 141
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 82  tgttagaatcaaatttactaattcatcaaaatgatacccacaataataatcatcatcatc 141

                                                                       
Query: 142 aagatgaaataacaaaatctgaaatcaaagttgcttcacctttaataaaacaagaattaa 201
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142 aagatgaaataacaaaatctgaaatcaaagttgcttcacctttaataaaacaagaattaa 201

                                                                       
Query: 202 gacaatctccaaaacaacttgaaagaaattctccacaaaatgataaagaattaacaattg 261
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 202 gacaatctccaaaacaacttgaaagaaattctccacaaaatgataaagaattaacaattg 261

                                                         
Query: 262 atgaaagtaatgatagtattgatactaccaatattggtagaaaaag 307
           ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 262 atgaaagtaatgatagtattgatactaccaatattggtagaaaaag 307


 Score =  287 bits (145), Expect = 2e-77
 Identities = 145/145 (100%)
 Strand = Plus / Plus

                                                                       
Query: 704 gagaattcgatagagtaattaccattcaatcaaacaactatgaaaaactattatcaaaga 763
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 704 gagaattcgatagagtaattaccattcaatcaaacaactatgaaaaactattatcaaaga 763

                                                                       
Query: 764 aatttcatgatatgaaaaaagatttaaatgaaaacactgatgccctatcaacttcatttg 823
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 764 aatttcatgatatgaaaaaagatttaaatgaaaacactgatgccctatcaacttcatttg 823

                                    
Query: 824 atattcttgataatattttaattag 848
           |||||||||||||||||||||||||
Sbjct: 824 atattcttgataatattttaattag 848


 Score =  167 bits (84), Expect = 5e-41
 Identities = 84/84 (100%)
 Strand = Plus / Plus

                                                                        
Query: 958  ctccctcctgtttacaataactcttcaaattttaattccaataattcaatgaatcaatac 1017
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 958  ctccctcctgtttacaataactcttcaaattttaattccaataattcaatgaatcaatac 1017

                                    
Query: 1018 tcatataataattatcatcaccac 1041
            ||||||||||||||||||||||||
Sbjct: 1018 tcatataataattatcatcaccac 1041


 Score = 73.8 bits (37), Expect = 5e-13
 Identities = 37/37 (100%)
 Strand = Plus / Plus

                                               
Query: 1  aaactcaattgaaaaaattaattgaaaaacttccaat 37
          |||||||||||||||||||||||||||||||||||||
Sbjct: 1  aaactcaattgaaaaaattaattgaaaaacttccaat 37


 Score = 32.2 bits (16), Expect = 1.7
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 286 ctaccaatattggtag 301
           ||||||||||||||||
Sbjct: 301 ctaccaatattggtag 286


 Score = 30.2 bits (15), Expect = 6.9
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                          
Query: 447 acaacaatttcaaca 461
           |||||||||||||||
Sbjct: 894 acaacaatttcaaca 908


>Contig-U12907-1 (Contig-U12907-1Q) /CSM_Contig/Contig-U12907-1Q.Seq.d
          Length = 2454

 Score = 44.1 bits (22), Expect = 5e-04
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                  
Query: 121  acaataataatcatcatcatca 142
            ||||||||||||||||||||||
Sbjct: 979  acaataataatcatcatcatca 1000


>Contig-U11017-1 (Contig-U11017-1Q) /CSM_Contig/Contig-U11017-1Q.Seq.d
          Length = 10637

 Score = 44.1 bits (22), Expect = 5e-04
 Identities = 25/26 (96%)
 Strand = Plus / Plus

                                       
Query: 420   gatgaagatgaagataatgatcaacc 445
             ||||| ||||||||||||||||||||
Sbjct: 10390 gatgatgatgaagataatgatcaacc 10415


  Database: CSM
    Posted date:  Jun 7, 2004  8:33 AM
  Number of letters in database: 7,895,291
  Number of sequences in database:  8361
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 31,935
Number of Sequences: 8361
Number of extensions: 31935
Number of successful extensions: 5202
Number of sequences better than 10.0: 499
length of query: 1129
length of database: 7,895,291
effective HSP length: 16
effective length of query: 1113
effective length of database: 7,761,515
effective search space: 8638566195
effective search space used: 8638566195
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 4.23
Homology vs DNA 
Query= Contig-U01123-1 (Contig-U01123-1Q) /CSM_Contig/Contig-U01123-1Q.Seq.d
         (1129 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU053299) Dictyostelium discoideum slug cDNA, clone SLI342.      428   e-145  2
(AU060746) Dictyostelium discoideum slug cDNA, clone SLB332.      448   e-131  2
(AU033700) Dictyostelium discoideum slug cDNA, clone SLB332.      287   e-114  2
(AU062207) Dictyostelium discoideum slug cDNA, clone SLI342.      200   8e-48  2
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    44   2e-04  9
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    36   0.002  14
(AC116985) Dictyostelium discoideum chromosome 2 map 6135249...    42   0.004  3
(AC005140) Plasmodium falciparum chromosome 12 clone PFYACB8...    44   0.008  2
(AC005139) Plasmodium falciparum chromosome 12, *** SEQUENCI...    44   0.008  2
(AE014851) Plasmodium falciparum 3D7 chromosome 12, section ...    44   0.008  2
(AC179680) Strongylocentrotus purpuratus clone R3-1021M22, W...    44   0.008  2
(AC181895) Strongylocentrotus purpuratus clone R3-1016C20, W...    46   0.036  4
(CI998177) Marsupenaeus japonicus cDNA, clone:YAQ01A02NGRM00...    38   0.060  3
(AC116960) Dictyostelium discoideum chromosome 2 map complem...    32   0.063  12
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...    38   0.098  10
(CP001184) Ureaplasma urealyticum serovar 10 str. ATCC 33699...    34   0.15   15
(AY575617) Aquilegia chrysantha clone A17 microsatellite seq...    36   0.15   2
(AF063866) Melanoplus sanguinipes entomopoxvirus, complete g...    32   0.18   10
(AC115598) Dictyostelium discoideum chromosome 2 map 581427-...    34   0.19   11
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    34   0.19   16
(CW917855) EDCA207TF A. castellanii, 6-8 kb library from tot...    50   0.19   1
(AF072715) Mycoplasma mycoides capri lipoprotein A precursor...    50   0.19   1
(AC179836) Strongylocentrotus purpuratus clone R3-1012D6, WO...    34   0.41   2
(GN007707) Sequence 148 from Patent EP2014669.                     36   0.41   2
(AC177955) Strongylocentrotus purpuratus clone R3-3049C24, W...    34   0.42   2
(AC178176) Strongylocentrotus purpuratus clone R3-1116L2, WO...    36   0.42   2
(CR925702) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    40   0.45   4
(AC092305) Homo sapiens chromosome 19 clone LLNLR-229B1, com...    36   0.45   2
(AC004710) Plasmodium falciparum chromosome 12, *** SEQUENCI...    34   0.47   8
(AL034392) Caenorhabditis elegans YAC Y40B1A.                      38   0.58   5
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    32   0.64   14
(AC123513) Dictyostelium discoideum strain AX4 chromosome 2 ...    36   0.67   7
(AU270341) Dictyostelium discoideum vegetative cDNA clone:VS...    34   0.68   3
(BD392540) A new promoter.                                         42   0.71   2
(AC095275) Rattus norvegicus clone CH230-11J20, *** SEQUENCI...    48   0.74   1
(AC174167) Strongylocentrotus purpuratus clone R3-30I1, WORK...    48   0.74   1
(AC168814) Strongylocentrotus purpuratus clone R3-13G18, WOR...    48   0.74   1
(EJ547642) 1092959301433 Global-Ocean-Sampling_GS-29-01-01-1...    48   0.74   1
(EJ529649) 1092955212455 Global-Ocean-Sampling_GS-29-01-01-1...    48   0.74   1
(EJ117266) 1092343358133 Global-Ocean-Sampling_GS-27-01-01-1...    48   0.74   1
(DX463328) Cpl05115 Papaya Genomic DNA BAC Library Carica pa...    48   0.74   1
(AC117082) Dictyostelium discoideum chromosome 2 map complem...    36   0.79   5
(AZ614134) 1M0442N17R Mouse 10kb plasmid UUGC1M library Mus ...    32   0.91   3
(CR382398) Plasmodium falciparum chromosome 6, complete sequ...    38   0.92   7
(AC117076) Dictyostelium discoideum chromosome 2 map 3323568...    34   1.00   11
(AB018112) Arabidopsis thaliana genomic DNA, chromosome 5, P...    38   1.1    5
(AC178989) Strongylocentrotus purpuratus clone R3-3122J2, WO...    42   1.2    9
(AC133388) Rattus norvegicus clone CH230-32A19, *** SEQUENCI...    40   1.3    3
(BJ376728) Dictyostelium discoideum cDNA clone:ddc29o13, 3' ...    38   1.3    3
(AR547390) Sequence 2521 from patent US 6747137.                   40   1.3    3
(AE014833) Plasmodium falciparum 3D7 chromosome 10 section 5...    36   1.5    2
(AC172324) Strongylocentrotus purpuratus clone R3-14I24, WOR...    36   1.5    2
(AC180912) Strongylocentrotus purpuratus clone R3-3026G14, W...    36   1.5    2
(AC175905) Strongylocentrotus purpuratus clone R3-14F6, WORK...    36   1.5    2
(AC102616) Mus musculus chromosome 18, clone RP23-425L21, co...    36   1.5    2
(AC157901) Mus musculus chromosome 18, clone RP23-189P21, co...    36   1.5    2
(AC195417) Pan troglodytes BAC clone CH251-141L7 from chromo...    36   1.5    2
(AC177660) Strongylocentrotus purpuratus clone R3-1017O13, W...    36   1.5    2
(AC176870) Strongylocentrotus purpuratus clone R3-1110G19, W...    34   1.5    2
(AC101638) Mus musculus clone RP23-199P13, LOW-PASS SEQUENCE...    36   1.6    2
(AE014823) Plasmodium falciparum 3D7 chromosome 14 section 8...    34   1.7    9
(AE014849) Plasmodium falciparum 3D7 chromosome 12, section ...    34   1.7    9
(Y13975) Candida albicans PLC1 gene.                               40   1.8    3
(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...    34   2.0    9
(CB284735) DF1526 Dermatophagoides farinae cDNA library Derm...    40   2.0    2
(FH152807) CHO_OF3064xd21r1.ab1 CHO_OF3 Nicotiana tabacum ge...    34   2.0    2
(EX927161) lk97a06.g7 Cycas ovule - normalized (NYBG) Cycas ...    36   2.0    2
(BM493033) NXRV_037_A06_F NXRV (Nsf Xylem Root wood Vertical...    38   2.0    2
(BM492733) NXRV_030_D08_F NXRV (Nsf Xylem Root wood Vertical...    38   2.0    2
(EY305899) CAWX19627.rev CAWX Helobdella robusta Primary Ear...    34   2.0    2
(EY318318) CAWX6319.rev CAWX Helobdella robusta Primary Earl...    34   2.0    2
(EY379806) CAXA17993.rev CAXA Helobdella robusta Subtracted ...    36   2.0    2
(EY301941) CAWX15865.rev CAWX Helobdella robusta Primary Ear...    34   2.0    2
(EY351029) CAWZ17902.rev CAWZ Helobdella robusta Primary Lat...    34   2.0    2
(EY310702) CAWX23065.rev CAWX Helobdella robusta Primary Ear...    34   2.0    2
(EY343135) CAWZ12319.rev CAWZ Helobdella robusta Primary Lat...    34   2.0    2
(FC659516) CAXW17234.fwd CAXW Lottia gigantea from female go...    36   2.0    2
(EY316050) CAWX26398.rev CAWX Helobdella robusta Primary Ear...    34   2.0    2
(DV827725) LB0209.CR_G20 GC_BGC-20 Bos taurus cDNA clone IMA...    36   2.0    2
(DR767495) ILLUMIGEN_MCQ_59993 Katze_MMOV Macaca mulatta cDN...    36   2.0    2
(EK289328) 1095462324460 Global-Ocean-Sampling_GS-31-01-01-1...    36   2.0    2
(EL508366) E11_PF-SAU3A-T3-M-P18_D.AB1 Blood stage Plasmodiu...    36   2.0    2
(EL508365) E11_PF-SAU3A-T3-M-P18.AB1 Blood stage Plasmodium ...    36   2.0    2
(AZ148912) SP_0048_A2_F05_T7 Strongylocentrotus purpuratus, ...    36   2.0    2
(BM274068) PfESToaa63d08.y1 Plasmodium falciparum 3D7 gameto...    36   2.0    2
(CR402610) Arabidopsis thaliana T-DNA flanking sequence GK-8...    34   2.1    2
(AL954709) Zebrafish DNA sequence from clone CH211-142N21 in...    34   2.2    7
(AL954331) Zebrafish DNA sequence from clone CH211-79E4 in l...    40   2.5    8
(EF710650) Glyptapanteles flavicoxis clone BAC 8G10, complet...    34   2.6    5
(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...    36   2.6    11
(AC157829) Strongylocentrotus purpuratus clone R3-13A6, WORK...    40   2.6    4
(BX897657) Zebrafish DNA sequence from clone DKEYP-84C2.           40   2.8    5
(AC123653) Mus musculus clone RP23-152M23, WORKING DRAFT SEQ...    42   2.9    4
(AC141069) Homo sapiens chromosome 16 clone RP11-155B7, WORK...    42   2.9    4
(AC005507) Plasmodium falciparum chromosome 12 clone PFYACB8...    32   2.9    11
(EJ097904) 1095462277444 Global-Ocean-Sampling_GS-26-01-01-1...    42   2.9    2
(CU457792) Zebrafish DNA sequence from clone DKEY-180K7 in l...    46   2.9    1
(BX548071) Zebrafish DNA sequence from clone DKEY-25L23 in l...    46   2.9    1
(EZ035299) TSA: Acropora millepora SeqIndex67122, mRNA seque...    46   2.9    1
(DL209306) Full length cDNA.                                       46   2.9    1
(DD036014) Full length cDNA.                                       46   2.9    1
(CQ843097) Sequence 1744 from Patent EP1440981.                    46   2.9    1
(DQ321784) Trichomonas vaginalis strain NIH-C1 MLH1-like pro...    46   2.9    1
(AF081829) Rhipicephalus sanguineus mitochondrial DNA, compl...    46   2.9    1
(AL049833) Human chromosome 14 DNA sequence BAC R-907D1 of l...    46   2.9    1
(AK126062) Homo sapiens cDNA FLJ44074 fis, clone TESTI4038258.     46   2.9    1
(AC151362) Ictalurus punctatus clone CH212-90E18, WORKING DR...    46   2.9    1
(BX511220) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    46   2.9    1
(AC023507) Homo sapiens chromosome 3 clone RP11-208K17, WORK...    46   2.9    1
(FH370064) CHO_OF4516xl11f1.ab1 CHO_OF4 Nicotiana tabacum ge...    46   2.9    1
(FH246210) CHO_OF3007xn12f1.ab1 CHO_OF3 Nicotiana tabacum ge...    46   2.9    1
(EJ593561) 1092961064862 Global-Ocean-Sampling_GS-29-01-01-1...    46   2.9    1
(EJ573935) 1092960079641 Global-Ocean-Sampling_GS-29-01-01-1...    46   2.9    1
(EI774062) PM1007G01TR BAC library from the prostate metasta...    46   2.9    1
(CE312028) tigr-gss-dog-17000360854478 Dog Library Canis lup...    46   2.9    1
(BZ508536) BONDK21TF BO_1.6_2_KB_tot Brassica oleracea genom...    46   2.9    1
(AV411685) Lotus japonicus cDNA clone:MWM209h08_r, 5' end.         46   2.9    1
(AU284044) Molgula tectiformis mRNA, clone:MT08B2F07T, 3' end.     46   2.9    1
(DN160357) GPN002C22u GPN Hordeum vulgare cDNA clone GPN002C...    46   2.9    1
(CJ451350) Macaca fascicularis mRNA, clone: QflA-16953, 5' e...    46   2.9    1
(CB284403) DF1148 Dermatophagoides farinae cDNA library Derm...    46   2.9    1
(GO192862) CAGB13789.fwd CAGB Alvinella pompejana Normalized...    46   2.9    1
(GO192861) CAGB13789.rev CAGB Alvinella pompejana Normalized...    46   2.9    1
(FG656277) G1142P323RP21.T0 Anolis carolinensis pooled norma...    46   2.9    1
(FG345328) SF_05-a04 Bv_T50 Beta vulgaris subsp. vulgaris cD...    46   2.9    1
(AC135272) Rattus norvegicus clone CH230-269J8, *** SEQUENCI...    40   3.0    3
(AC181143) Strongylocentrotus purpuratus clone R3-16E2, WORK...    40   3.1    3
(AL844509) Plasmodium falciparum chromosome 13.                    36   3.1    15
(AC177300) Strongylocentrotus purpuratus clone R3-45A14, WOR...    40   3.6    4
(CT967319) M.truncatula DNA sequence from clone MTH2-59J17 o...    36   4.0    6
(CP000942) Ureaplasma parvum serovar 3 str. ATCC 27815, comp...    34   4.1    15
(AF222894) Ureaplasma parvum serovar 3 str. ATCC 700970, com...    34   4.1    15
(AC171728) Solanum lycopersicum chromosome 1 clone C01HBa021...    42   4.2    7
(AC068631) Homo sapiens 3 BAC RP11-134F2 (Roswell Park Cance...    38   4.7    4
(AB030033) Dictyostelium discoideum gene for AmiB, complete ...    36   4.8    4
(AL844501) Plasmodium falciparum strain 3D7, chromosome 1.         36   5.2    10
(AP006103) Lotus japonicus genomic DNA, chromosome 1, clone:...    36   5.3    8
(AC074161) Mus musculus clone RP23-32A10, WORKING DRAFT SEQU...    32   5.4    2
(AC209577) Mus musculus BAC clone RP23-206M14 from chromosom...    32   5.5    2
(AC168800) Strongylocentrotus purpuratus clone R3-13I15, WOR...    32   5.5    2
(AC125624) Homo sapiens 3 BAC RP11-42O1 (Roswell Park Cancer...    34   5.5    2
(AC026852) Homo sapiens chromosome 3 clone RP11-42O1, *** SE...    34   5.5    2
(AC176017) Strongylocentrotus purpuratus clone R3-17O4, WORK...    32   5.5    2
(AC154425) Mus musculus BAC clone RP24-196O13 from chromosom...    32   5.5    2
(AC175894) Strongylocentrotus purpuratus clone R3-15A19, WOR...    32   5.6    2
(AC201554) Strongylocentrotus purpuratus clone R3-3118H22, W...    32   5.6    2
(CU595936) Zebrafish DNA sequence from clone CH73-169K22 in ...    40   5.9    4
(CT573506) Medicago truncatula chromosome 5 clone mth4-42d11...    36   6.6    6
(AC179115) Strongylocentrotus purpuratus clone R3-4011G4, WO...    36   6.7    7
(AC216447) Populus trichocarpa clone ACSB1045-A16, complete ...    34   7.2    3
(AC196104) Zea mays chromosome 8 clone CH201-326A6; ZMMBBc03...    40   7.4    2
(EY388343) CAXA7252.fwd CAXA Helobdella robusta Subtracted L...    32   7.4    2
(EJ107081) 1092343140226 Global-Ocean-Sampling_GS-27-01-01-1...    32   7.4    2
(BH162866) ENTQV64TF Entamoeba histolytica Sheared DNA Entam...    32   7.4    2
(EK412304) 1095505203492 Global-Ocean-Sampling_GS-31-01-01-1...    34   7.4    2
(CX080131) EHAA472TR E. histolytica Normalized cDNA library ...    32   7.4    2
(AZ686732) ENTLI81TR Entamoeba histolytica Sheared DNA Entam...    32   7.4    2
(EY318643) CAWX6481.rev CAWX Helobdella robusta Primary Earl...    34   7.4    2
(EJ883458) 1093018406612 Global-Ocean-Sampling_GS-30-02-01-1...    32   7.4    2
(EY300829) CAWX15237.rev CAWX Helobdella robusta Primary Ear...    34   7.4    2
(EY370432) CAXA12911.fwd CAXA Helobdella robusta Subtracted ...    32   7.4    2
(CX085358) EHAC436TR E. histolytica Normalized cDNA library ...    32   7.4    2
(EY345501) CAWZ13986.fwd CAWZ Helobdella robusta Primary Lat...    32   7.4    2
(CX093455) EHAFL35TR E. histolytica Normalized cDNA library ...    32   7.4    2
(BX950864) Zebrafish DNA sequence from clone DKEY-158B13 in ...    40   7.4    5
(CX097370) EHAH587TR E. histolytica Normalized cDNA library ...    32   7.4    2
(EY359642) CAWZ6602.rev CAWZ Helobdella robusta Primary Late...    32   7.5    2
(CO638878) USDA-FP_107615 Adult Glassy-winged Sharpshooter H...    34   7.5    2
(CR917797) Sus scrofa BES.                                         34   7.5    2
(FM009286) Dicentrarchus labrax EST, clone cDN26P0002K16, 5'...    34   7.5    2
(EJ427265) 1093015233713 Global-Ocean-Sampling_GS-28-01-01-1...    32   7.5    2
(AC009820) Homo sapiens chromosome 11 clone RP11-397P10 map ...    42   7.6    5
(AL929351) Plasmodium falciparum strain 3D7, chromosome 5, s...    44   7.6    11
(EK163645) 1095458042025 Global-Ocean-Sampling_GS-31-01-01-1...    34   7.6    2
(CU459001) Zebrafish DNA sequence from clone CH73-146D21 in ...    40   7.7    6
(CR352217) Zebrafish DNA sequence from clone CH211-129P13 in...    40   7.9    3
(AL935322) Zebrafish DNA sequence from clone CH211-239B20 in...    40   7.9    3
(AL360087) Human DNA sequence from clone RP11-558G9 on chrom...    36   8.0    4
(FE753167) CAYF2792.g1 CAYF Petrolisthes cinctipes heart, wh...    42   8.0    2
(AC180691) Strongylocentrotus purpuratus clone R3-3087A21, W...    40   8.2    5
(FE813527) CCAG31126.g1 CCAG Petrolisthes cinctipes heart, g...    42   8.2    2
(AE017263) Mesoplasma florum L1 complete genome.                   36   8.4    16
(CR382400) Plasmodium falciparum chromosome 6, complete sequ...    34   8.6    12
(FE782040) CCAG12408.g1 CCAG Petrolisthes cinctipes heart, g...    42   8.8    2
(FE811376) CCAG29771.g1 CCAG Petrolisthes cinctipes heart, g...    42   9.0    2
(AC117080) Dictyostelium discoideum chromosome 2 map complem...    32   9.3    7
(AL022576) Human DNA sequence from clone RP1-20J23 on chromo...    44   9.3    4
(AC168374) Strongylocentrotus purpuratus clone R3-4013I8, WO...    38   9.3    4
(FE788167) CCAG15953.b5 CCAG Petrolisthes cinctipes heart, g...    42   9.6    2
(ER446129) 1092963830020 Global-Ocean-Sampling_GS-35-01-01-1...    38   9.6    2
(FE782039) CCAG12408.b3 CCAG Petrolisthes cinctipes heart, g...    42   9.7    2
(FE772228) CCAG6535.g1 CCAG Petrolisthes cinctipes heart, gi...    42   9.7    2
(DX476834) Cpl19069 Papaya Genomic DNA BAC Library Carica pa...    40   9.7    2
(FE717940) SB03066A2A09.f1 Normalized subtracted Keck-Tagu L...    42   9.8    2
(EJ002352) 1091120930604 Global-Ocean-Sampling_GS-26-01-01-1...    36   9.8    2
(FE753166) CAYF2792.b1 CAYF Petrolisthes cinctipes heart, wh...    42   9.9    2
(AC184399) Strongylocentrotus purpuratus clone R3-3112C9, WO...    38   9.9    6
(FE789010) CCAG16434.b3 CCAG Petrolisthes cinctipes heart, g...    42   9.9    2

>(AU053299) Dictyostelium discoideum slug cDNA, clone SLI342.
          Length = 454

 Score =  428 bits (216), Expect(2) = e-145
 Identities = 216/216 (100%)
 Strand = Plus / Plus


Query: 414 ctcattgatgaagatgaagataatgatcaacctacaacaatttcaacatcaataataaaa 473
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 174 ctcattgatgaagatgaagataatgatcaacctacaacaatttcaacatcaataataaaa 233


Query: 474 aatgatgatgataagaatgaccaaatgaaattgaaaataacattctcaaagaaatcatct 533
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 234 aatgatgatgataagaatgaccaaatgaaattgaaaataacattctcaaagaaatcatct 293


Query: 534 gatcttaacttaacaacaagtagtgactcatccaatccaacagttgtatctccgatggcg 593
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 294 gatcttaacttaacaacaagtagtgactcatccaatccaacagttgtatctccgatggcg 353


Query: 594 aacgatgatgctgagactggaaggaaggaaattatt 629
           ||||||||||||||||||||||||||||||||||||
Sbjct: 354 aacgatgatgctgagactggaaggaaggaaattatt 389

 Score =  125 bits (63), Expect(2) = e-145
 Identities = 66/67 (98%)
 Strand = Plus / Plus


Query: 241 atgataaagaattaacaattgatgaaagtaatgatagtattgatactaccaatattggta 300
           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct: 1   atgataaagaattaacaattgatgaaagtaatgatagtattgctactaccaatattggta 60


Query: 301 gaaaaag 307
           |||||||
Sbjct: 61  gaaaaag 67

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 1,107,286,344
Number of extensions: 78123048
Number of successful extensions: 7691218
Number of sequences better than 10.0: 203
Length of query: 1129
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 1105
Effective length of database: 99,340,224,878
Effective search space: 109770948490190
Effective search space used: 109770948490190
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.14
Homology vs Protein 
Query= Contig-U01123-1 (Contig-U01123-1Q) /CSM_Contig/Contig-U01123-1Q.Seq.d
         (1129 letters)

Database: nrp_B
           3,236,559 sequences; 1,051,180,864 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001056_3217(CP001056|pid:none) Clostridium botulinum B str. Ek...    36   2.9

>CP001056_3217(CP001056|pid:none) Clostridium botulinum B str.
           Eklund 17B, complete genome.
          Length = 339

 Score = 35.8 bits (81), Expect = 2.9
 Identities = 15/43 (34%), Positives = 28/43 (65%)
 Frame = +3

Query: 390 IVKKDNKKLIDEDEDNDQPTTISTSIIKNDDDKNDQMKLKITF 518
           I+KK NKK++   + N++    + S IK D+DK+++++L   F
Sbjct: 195 IMKKSNKKIVIIKDSNNKEILTTKSDIKKDEDKDNEIRLNFNF 237

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 761,585,091
Number of extensions: 7955875
Number of successful extensions: 30070
Number of sequences better than 10.0: 1
Number of HSP's gapped: 29792
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 1,051,180,864
Length adjustment: 130
Effective length of query: 246
Effective length of database: 630,428,194
Effective search space: 155085335724
Effective search space used: 155085335724
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.64  gvh: 0.43  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for Contig-U01123-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 2
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0